DOCSLIB.ORG

Biophysical and Cellular Mechanisms of Tip-Growth in the Brown Alga Ectocarpus Sp

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Biophysical and Cellular Mechanisms of Tip-Growth in the Brown Alga Ectocarpus Sp Biophysical and cellular mechanisms of tip-growth in the brown alga Ectocarpus sp. Herve Rabille To cite this version: Herve Rabille. Biophysical and cellular mechanisms of tip-growth in the brown alga Ectocarpus sp.. Cellular Biology. Sorbonne Université, 2018. English. NNT : 2018SORUS597. tel-02489811 HAL Id: tel-02489811 https://tel.archives-ouvertes.fr/tel-02489811 Submitted on 24 Feb 2020 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés. Sorbonne Université Ecole doctorale 515 Complexité du Vivant UMR 8227 (SU – CNRS) Laboratoire de Biologie Intégrative des Modèles Marins / Equipe de recherche « Morphogenesis of Macroalgae » Biophysical and cellular mechanisms of tip-growth in the brown alga Ectocarpus sp. Par Hervé Rabillé Thèse de doctorat de Biologie Dirigée par Dr. Bénédicte Charrier Présentée et soutenue publiquement le 3 décembre 2018 Devant un jury composé de : M. Arezki Boudaoud, Professeur de l’ENS de Lyon, France : Rapporteur Mme. Siobhan Braybrook, Project Investigator at the University California Los Angeles, USA : Rapporteuse M. Benedikt Kost, Professeur de l’Université Friedrich Alexander Universität, Erlangen, Allemagne : Examinateur M. Bernard Kloareg, Professeur de Sorbonne Université de Paris, France : Examinateur Mme Bénédicte Charrier, Directrice de Recherche CNRS : Directrice de thèse Rabillé Hervé – Thèse de doctorat – 2018 Biophysical and cellular mechanisms of tip-growth in Ectocarpus Sorbonne Université Ecole doctorale 515 Complexité du Vivant UMR 8227 (SU – CNRS) Laboratoire de Biologie Intégrative des Modèles Marins / Equipe de recherche « Morphogenesis of Macroalgae » Biophysical and cellular mechanisms of tip-growth in the brown alga Ectocarpus sp. Par Hervé Rabillé Thèse de doctorat de Biologie Dirigée par Dr. Bénédicte Charrier Présentée et soutenue publiquement le 3 décembre 2018 Devant un jury composé de : M. Arezki Boudaoud, Professeur de l’ENS de Lyon, France : Rapporteur Mme. Siobhan Braybrook, Project Investigator at the University California Los Angeles, USA : Rapporteuse M. Benedikt Kost, Professeur de l’Université Friedrich Alexander Universität, Erlangen, Allemagne : Examinateur M. Bernard Kloareg, Professeur de Sorbonne Université de Paris, France : Examinateur Mme Bénédicte Charrier, Directrice de Recherche CNRS : Directrice de thèse 1 Biophysical and cellular mechanisms of tip-growth in Ectocarpus 2 Biophysical and cellular mechanisms of tip-growth in Ectocarpus Preamble Contribution This thesis project is by no means the only product of my own work, but is instead the fruit of the collective work of many people. The contribution of each is detailed below. At first, the project has involved the effort of all the members of the Morphogenesis of MacroAlgae research team. Dr Bénédicte Charrier, researcher in plant biology, performed a substantial part of the cytology experiments and data analysis. Dr Bernard Billoud, lecturer in bioinformatics, contributed to the analysis of some quantitative data and carried out all the computational work related to the mechanical modelling of Ectocarpus tip-growth. Élodie Rolland, research technician in tissue culture, performed most of the algae cultivation tasks, especially the preparation of the parthenosporophytes of Ectocarpus grown on glass coverslips, which were essential for most of the experiments. The staining of the actin cytoskeleton using the phalloidin-based probe has been performed in collaboration with Pr. Christos Katsaros and his PhD student Maria Koutalianou, both from the National and Kapodistrian University of Athens (Greece). Actin and tubulin immunolocalisation experiments have been carried out with Dr. Adeel Nasir (Friedrich Alexander Universität, Erlangen-Nurnberg, Germany), who supplied us with an alternative protocol for cytoskeleton staining during a short visit. All the observations of cell ultrastructure by Transmission Electronic Microscopy (TEM) have been conducted by Dr. Sophie Le Panse, from the “MerImage” microscopy plateform at the Roscoff Marine Biology Station. The AFM data that are briefly presented and discussed in this report have been acquired by Benoit Tesson (Scripps Institution of Oceanography, University of California, San Diego, USA). The identification of the causal mutation in the mutant étoile (etl) and the bioinformatic analysis (with the help of B. Billoud) of the candidate ETOILE gene which is briefly discussed in this report, is mostly the work of Zofia Nehr (former PhD student in the team). I performed the final completion of her substantial work with the help of the L2 student Quentin Rochas, whom I supervised. Finally, the results presented on the effect of drugs depolymerizing the cytoskeleton on the growth and morphogenesis of the apical cell were from time-lapse pictures of Ectocarpus filaments grown by Carole Duchêne, a former L3 internship in our team. Articles The Part 1 (Introduction section) of the report contains a large chapter reviewing the biomechanical models of tip-growth across the tree of life. It will be submitted as a review for an annual series or a book (Rabillé & Charrier, in preparation). The Part 1 also comprises an Opinion paper discussing the extent to which the cell wall composition and its intrinsic mechanics impact growth (Charrier, Rabillé & Billoud, in press in Trends in Plant Science). The Part 2 deals with the biomechanics of tip-growth and of the cell wall. It contains two Original Research papers. The first article (Part 2.1) presents a visco-elastic model accounting for the tip growth in Ectocarpus, highlighting the role of the cell wall thickness 3 Biophysical and cellular mechanisms of tip-growth in Ectocarpus (Rabillé et al., in revision in PLoS Biology). The second one (Part 2.2) reports the role of alginates in the mechanics of the cell wall along the filament of Ectocarpus (Rabillé et al., in preparation). 4 Biophysical and cellular mechanisms of tip-growth in Ectocarpus Acknowledgements First I must thank the two organisms that have funded my PhD: The Presidency of the former Université Pierre and Marie Curie (now fused with Sorbonne Université) and the Brittany Region. I am also indebted to the Phycomorph Network (EC funded COST Action FA1406) for having funding my Short-Term Scientific Mission from the 12th to the 29th of October, 2016, in the laboratory of Pr. Christos Katsaros (see below). I thank warmly Dr Bénédicte Charrier, head of the Morphogenesis of MacroAlgae (MMA) team and my PhD supervisor, for having given me the opportunity to do work on such an exciting research project, at the very forefront of the current knowledge in cellular and developmental biology. Thank also you a lot for all the time you have dedicated to supervising my thesis, for all the (often very long) theoretical discussions about the mechanisms of cell growth, and especially on wall expansion mechanisms, the very hot topic that repeatedly came at the center of our thoughts in the course of these three years. Also, thank you for founding the very last weeks of this thesis project. In the same vein, I have to thank you a lot, Dr Bernard Billoud, for your dedication into the project, and for the HUGE work for the numerical modelling of Ectocarpus tip-growth. Thank you also for, again, all the theoretical discussions mentioned above, and for you many informed, enlightened, and often stated loud and clear, points of view on the numerous hot technical and theoretical issues this research project has given rise to. More generally, I would like to tell all my gratitude to both B. Charrier and B. Billoud, for the overwhelming work that was the redaction and formatting of the two original papers which drafts are included in this report, in which I have only played a small part. I also would like to thank Dr Catherine Boyen for having funded one extra month and a half of my thesis, that have allowed me to have time to write my report (extra time that I have largely abused). I would also like to thanks all the member of my thesis committee, for their GREAT patience and indulgence (my apologies, again, for the almost 2 hours’ lateness at the second meeting, and my complete lack of professionalism), for their interest in the project and their kind pieces of advice. So thanks to Pr Bruno De Reviers, Dr Hayat Bouteau, Dr Thierry Comtet. Thank also to Dr Arezki Boudaoud and Dr. B. Billoud for attending the first meeting in February 2017. I thank also all the members of my thesis comitee, that have kindly accepted to attend and evaluate my future thesis defence, that is to take place on the 3rd of December 2018. So thank you Dr Siobhan Braybrook, Dr Arezki Boudaoud, Dr Bernard Kloareg and Dr Benedikt Kost. Many thanks especially for two former for tackling the task of evaluating this huge report. Here and now, I would like to thanks all the other people that have got involved in the thesis project, and without whom nothing, or so few, would have been possible. First I would like to thanks Élodie Rolland, research technician in the team, for preparing loads of Ectocarpus cultures necessary
Load more

Recommended publications
  • Algae & Marine Plants of Point Reyes
    Algae & Marine Plants of Point Reyes Green Algae or Chlorophyta Genus/Species Common Name Acrosiphonia coalita Green rope, Tangled weed Blidingia minima Blidingia minima var. vexata Dwarf sea hair Bryopsis corticulans Cladophora columbiana Green tuft alga Codium fragile subsp. californicum Sea staghorn Codium setchellii Smooth spongy cushion, Green spongy cushion Trentepohlia aurea Ulva californica Ulva fenestrata Sea lettuce Ulva intestinalis Sea hair, Sea lettuce, Gutweed, Grass kelp Ulva linza Ulva taeniata Urospora sp. Brown Algae or Ochrophyta Genus/Species Common Name Alaria marginata Ribbon kelp, Winged kelp Analipus japonicus Fir branch seaweed, Sea fir Coilodesme californica Dactylosiphon bullosus Desmarestia herbacea Desmarestia latifrons Egregia menziesii Feather boa Fucus distichus Bladderwrack, Rockweed Haplogloia andersonii Anderson's gooey brown Laminaria setchellii Southern stiff-stiped kelp Laminaria sinclairii Leathesia marina Sea cauliflower Melanosiphon intestinalis Twisted sea tubes Nereocystis luetkeana Bull kelp, Bullwhip kelp, Bladder wrack, Edible kelp, Ribbon kelp Pelvetiopsis limitata Petalonia fascia False kelp Petrospongium rugosum Phaeostrophion irregulare Sand-scoured false kelp Pterygophora californica Woody-stemmed kelp, Stalked kelp, Walking kelp Ralfsia sp. Silvetia compressa Rockweed Stephanocystis osmundacea Page 1 of 4 Red Algae or Rhodophyta Genus/Species Common Name Ahnfeltia fastigiata Bushy Ahnfelt's seaweed Ahnfeltiopsis linearis Anisocladella pacifica Bangia sp. Bossiella dichotoma Bossiella
    [Show full text]
  • The Marine Life Information Network® for Britain and Ireland (Marlin)
    The Marine Life Information Network® for Britain and Ireland (MarLIN) Description, temporal variation, sensitivity and monitoring of important marine biotopes in Wales. Volume 1. Background to biotope research. Report to Cyngor Cefn Gwlad Cymru / Countryside Council for Wales Contract no. FC 73-023-255G Dr Harvey Tyler-Walters, Charlotte Marshall, & Dr Keith Hiscock With contributions from: Georgina Budd, Jacqueline Hill, Will Rayment and Angus Jackson DRAFT / FINAL REPORT January 2005 Reference: Tyler-Walters, H., Marshall, C., Hiscock, K., Hill, J.M., Budd, G.C., Rayment, W.J. & Jackson, A., 2005. Description, temporal variation, sensitivity and monitoring of important marine biotopes in Wales. Report to Cyngor Cefn Gwlad Cymru / Countryside Council for Wales from the Marine Life Information Network (MarLIN). Marine Biological Association of the UK, Plymouth. [CCW Contract no. FC 73-023-255G] Description, sensitivity and monitoring of important Welsh biotopes Background 2 Description, sensitivity and monitoring of important Welsh biotopes Background The Marine Life Information Network® for Britain and Ireland (MarLIN) Description, temporal variation, sensitivity and monitoring of important marine biotopes in Wales. Contents Executive summary ............................................................................................................................................5 Crynodeb gweithredol ........................................................................................................................................6
    [Show full text]
  • Oxygenated Acyclic Diterpenes with Anticancer Activity from the Irish Brown Seaweed Bifurcaria Bifurcata
    marine drugs Article Oxygenated Acyclic Diterpenes with Anticancer Activity from the Irish Brown Seaweed Bifurcaria bifurcata Vangelis Smyrniotopoulos 1, Christian Merten 2, Daria Firsova 1, Howard Fearnhead 3 and Deniz Tasdemir 1,4,5,* 1 School of Chemistry, National University of Ireland Galway, University Road, H91 TK33 Galway, Ireland; [email protected] (V.S.); dashafi[email protected] (D.F.) 2 Organische Chemie 2, Ruhr-Universität Bochum, Universitätsstraße 150, 44801 Bochum, Germany; [email protected] 3 Pharmacology and Therapeutics, School of Medicine, National University of Ireland Galway, University Road, H91 W2TY Galway, Ireland; [email protected] 4 GEOMAR Centre for Marine Biotechnology (GEOMAR-Biotech), Research Unit Marine Natural Product Chemistry, GEOMAR Helmholtz Centre for Ocean Research Kiel, Am Kiel-Kanal 44, 24106 Kiel, Germany 5 Faculty of Mathematics and Natural Sciences, Kiel University, Christian-Albrechts-Platz 4, 24118 Kiel, Germany * Correspondence: [email protected]; Tel.: +49-431-600-4430 Received: 28 October 2020; Accepted: 19 November 2020; Published: 23 November 2020 Abstract: Brown alga Bifurcaria bifurcata is a prolific source of bioactive acyclic (linear) diterpenes with high structural diversity. In the continuation of our investigations on Irish brown algae, we undertook an in-depth chemical study on the n-hexanes and chloroform subextracts of B. bifurcata that led to isolation of six new (1–6) and two known (7–8) acyclic diterpenes. Chemical structures of the compounds were elucidated by a combination of 1D and 2D NMR, HRMS, FT-IR, [α]D and vibrational circular dichroism (VCD) spectroscopy. Compounds 1–8, as well as three additional linear diterpenes (9–11), which we isolated from the same seaweed before, were tested against the human breast cancer cell line (MDA-MB-231).
    [Show full text]
  • Universidade Federal Do Estado Do Rio De Janeiro
    UNIVERSIDADE FEDERAL DO ESTADO DO RIO DE JANEIRO - UNIRIO CENTRO DE CIÊNCIAS BIOLÓGICAS E DA SAÚDE - CCBS INSTITUTO DE BIOCIÊNCIAS - IBio PROGRAMA DE PÓS-GRADUAÇÃO EM CIÊNCIAS BIOLÓGICAS - PPGBIO (BIODIVERSIDADE NEOTROPICAL) Erick Alves Pereira Lopes Filho Filogenia e filogeografia de espécies de Dictyota Lamouroux (Dictyotales: Phaeophyceae) Rio de Janeiro 2018 Erick Alves Pereira Lopes Filho Filogenia e filogeografia de espécies de Dictyota Lamouroux (Dictyotales: Phaeophyceae) Dissertação apresentada ao Programa de Pós-Graduação em Ciências Biológicas (Biodiversidade Neotropical) da Universidade Federal do Estado do Rio de Janeiro como requisito parcial para obtenção do título de Mestre. Orientador: Prof. Dr. Joel Campos de Paula Co-orientador: Prof. Dr. Fabiano Salgueiro Rio de Janeiro 2018 UNIVERSIDADE FEDERAL DO ESTADO DO RIO DE JANEIRO - UNIRIO CENTRO DE CIÊNCIAS BIOLÓGICAS E DA SAÚDE - CCBS INSTITUTO DE BIOCIÊNCIAS - IBio Erick Alves Pereira Lopes Filho Filogenia e filogeografia de espécies de Dictyota Lamouroux (Dictyotales: Phaeophyceae) Dissertação apresentada ao curso de Mestrado em Ciências Biológicas do Programa de Pós- Graduação em Ciências Biológicas (Biodiversidade Neotropical) da Universidade Federal do Estado do Rio de Janeiro no dia 11 de janeiro de 2018, como requisito parcial para a obtenção do título de Mestre em Ciências Biológicas. A mesma foi avaliada pela banca examinadora composta por Dr.ª Maria Beatriz Barbosa de Barros Barreto, Dr.ª Valéria Cassano e Dr. Joel Campos de Paula, sendo suplentes Dr. Fabiano Salgueiro, Dr. Leandro Pederneiras e Dr.ª Valéria Laneuville Teixeira, e aprovada com o conceito _________________ Dr.ª Maria Beatriz Barbosa de Barros Barreto Universidade Federal do Rio de Janeiro ______ Dr.ª Valéria Cassano Universidade de São Paulo Dr.
    [Show full text]
  • BROWN ALGAE [147 Species] (
    CHECKLIST of the SEAWEEDS OF IRELAND: BROWN ALGAE [147 species] (http://seaweed.ucg.ie/Ireland/Check-listPhIre.html) PHAEOPHYTA: PHAEOPHYCEAE ECTOCARPALES Ectocarpaceae Acinetospora Bornet Acinetospora crinita (Carmichael ex Harvey) Kornmann Dichosporangium Hauck Dichosporangium chordariae Wollny Ectocarpus Lyngbye Ectocarpus fasciculatus Harvey Ectocarpus siliculosus (Dillwyn) Lyngbye Feldmannia Hamel Feldmannia globifera (Kützing) Hamel Feldmannia simplex (P Crouan et H Crouan) Hamel Hincksia J E Gray - Formerly Giffordia; see Silva in Silva et al. (1987) Hincksia granulosa (J E Smith) P C Silva - Synonym: Giffordia granulosa (J E Smith) Hamel Hincksia hincksiae (Harvey) P C Silva - Synonym: Giffordia hincksiae (Harvey) Hamel Hincksia mitchelliae (Harvey) P C Silva - Synonym: Giffordia mitchelliae (Harvey) Hamel Hincksia ovata (Kjellman) P C Silva - Synonym: Giffordia ovata (Kjellman) Kylin - See Morton (1994, p.32) Hincksia sandriana (Zanardini) P C Silva - Synonym: Giffordia sandriana (Zanardini) Hamel - Only known from Co. Down; see Morton (1994, p.32) Hincksia secunda (Kützing) P C Silva - Synonym: Giffordia secunda (Kützing) Batters Herponema J Agardh Herponema solitarium (Sauvageau) Hamel Herponema velutinum (Greville) J Agardh Kuetzingiella Kornmann Kuetzingiella battersii (Bornet) Kornmann Kuetzingiella holmesii (Batters) Russell Laminariocolax Kylin Laminariocolax tomentosoides (Farlow) Kylin Mikrosyphar Kuckuck Mikrosyphar polysiphoniae Kuckuck Mikrosyphar porphyrae Kuckuck Phaeostroma Kuckuck Phaeostroma pustulosum Kuckuck
    [Show full text]
  • New Records of Benthic Brown Algae (Ochrophyta) from Hainan Island (1990 - 2016)
    Titlyanova TV et al. Ochrophyta from Hainan Data Paper New records of benthic brown algae (Ochrophyta) from Hainan Island (1990 - 2016) Tamara V. Titlyanova1, Eduard A. Titlyanov1, Li Xiubao2, Bangmei Xia3, Inka Bartsch4 1National Scientific Centre of Marine Biology, Far Eastern Branch of the Russian Academy of Sciences, Palchevskogo 17, Vladivostok, 690041, Russia; 2Key Laboratory of Tropical Marine Bio-Resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China; 3Institute of Oceanology, Chinese Academy of Sciences, 7 Nanhai Road, 266071 Qingdao, PR China; 4Alfred-Wegener-Institute for Polar and Marine Research, Am Handelshafen 12, 27570 Bremerhaven, Germany Corresponding author: E Titlyanov, e-mail: [email protected] Abstract This study reports on the intertidal and shallow subtidal brown algal flora from Hainan Island in the South China Sea, based on extensive sample collection conducted in 1990, 1992 and 2008−2016. The analysis revealed 27 new records of brown algae for Hainan Island, including 5 species which also constitute new records for China. 21 of these species are de- scribed with photographs and an annotated list of all species with information on life forms, habitat (localities and tidal zones) and their geographical distribution is provided. Keywords: Hainan Island, new records, seaweeds, brown algae Introduction et al. 1994; Hodgson & Yau 1997; Tadashi et al. 2008). Overall, algal species richness also changed. Hainan Island is located on the subtropical northern Partial inventory of the benthic flora of Hainan has periphery of the Pacific Ocean in the South China Sea already been carried out (Titlyanov et al. 2011a, 2015, 2016; (18˚10′-20˚9′ N, 108˚37′-111˚1′ E).
    [Show full text]
  • Ectocarpus: an Evo‑Devo Model for the Brown Algae Susana M
    Coelho et al. EvoDevo (2020) 11:19 https://doi.org/10.1186/s13227-020-00164-9 EvoDevo REVIEW Open Access Ectocarpus: an evo-devo model for the brown algae Susana M. Coelho1* , Akira F. Peters2, Dieter Müller3 and J. Mark Cock1 Abstract Ectocarpus is a genus of flamentous, marine brown algae. Brown algae belong to the stramenopiles, a large super- group of organisms that are only distantly related to animals, land plants and fungi. Brown algae are also one of only a small number of eukaryotic lineages that have evolved complex multicellularity. For many years, little information was available concerning the molecular mechanisms underlying multicellular development in the brown algae, but this situation has changed with the emergence of Ectocarpus as a model brown alga. Here we summarise some of the main questions that are being addressed and areas of study using Ectocarpus as a model organism and discuss how the genomic information, genetic tools and molecular approaches available for this organism are being employed to explore developmental questions in an evolutionary context. Keywords: Ectocarpus, Life-cycle, Sex determination, Gametophyte, Sporophyte, Brown algae, Marine, Complex multicellularity, Phaeoviruses Natural habitat and life cycle Ectocarpus is a cosmopolitan genus, occurring world- Ectocarpus is a genus of small, flamentous, multicellu- wide in temperate and subtropical regions, and has been lar, marine brown algae within the order Ectocarpales. collected on all continents except Antarctica [1]. It is pre- Brown algae belong to the stramenopiles (or Heter- sent mainly on rocky shores where it grows on abiotic okonta) (Fig. 1a), a large eukaryotic supergroup that (rocks, pebbles, dead shells) and biotic (other algae, sea- is only distantly related to animals, plants and fungi.
    [Show full text]
  • Dictyotales, Phaeophyceae)1
    ƒ. Phycol. 46, 1301-1321 (2010) © 2010 Phycological Society of America DOI: 10.1 lll/j.1529-8817.2010.00908.x SPECIES DELIMITATION, TAXONOMY, AND BIOGEOGRAPHY OF D ICTYO TA IN EUROPE (DICTYOTALES, PHAEOPHYCEAE)1 Ana Tronholn? Departamento de Biología Vegetal (Botánica) , Universidad de La Laguna, 38271 La Laguna, Canary Islands, Spain Frederique Steen, Lennert Tyberghein, Frederik Leliaert, Heroen Verbruggen Phycology Research Group and Centre for Molecular Phylogenetics and Evolution, Ghent University, Rrijgslaan 281, Building S8, 9000 Ghent, Belgium M. Antonia Ribera Signan Unitat de Botánica, Facultat de Farmacia, Universität de Barcelona, Joan XXIII s/n, 08032 Barcelona, Spain and Olivier De Clerck Phycology Research Group and Centre for Molecular Phylogenetics and Evolution, Ghent University, Rrijgslaan 281, Building S8, 9000 Ghent, Belgium Taxonomy of the brown algal genus Dictyota has a supports the by-product hypothesis of reproductive long and troubled history. Our inability to distin­ isolation. guish morphological plasticity from fixed diagnostic Key index words: biogeography; Dictyota; Dictyotales; traits that separate the various species has severely diversity; molecular phylogenetics; taxonomy confounded species delineation. From continental Europe, more than 60 species and intraspecific taxa Abbreviations: AIC, Akaike information criterion; have been described over the last two centuries. Bí, Bayesian inference; BIC, Bayesian information Using a molecular approach, we addressed the criterion; GTR, general time reversible; ML, diversity of the genus in European waters and made maximum likelihood necessary taxonomic changes. A densely sampled DNA data set demonstrated the presence of six evo- lutionarily significant units (ESUs): Dictyota dichotoma Species of the genus Dictyota J. V. Lamour., along (Huds.) J. V.
    [Show full text]
  • Genetic Diversity of Ectocarpus (Ectocarpales, Phaeophyceae) in Peru and Northern Chile, the Area of Origin of the Genome-Sequenced Strain Akira F
    Genetic diversity of Ectocarpus (Ectocarpales, Phaeophyceae) in Peru and northern Chile, the area of origin of the genome-sequenced strain Akira F. Peters, Aaron D. Mann, César A. Cordova, Juliet Brodie, Juan A. Correa, Declan C. Schroeder, J. Mark Cock To cite this version: Akira F. Peters, Aaron D. Mann, César A. Cordova, Juliet Brodie, Juan A. Correa, et al.. Genetic diversity of Ectocarpus (Ectocarpales, Phaeophyceae) in Peru and northern Chile, the area of origin of the genome-sequenced strain. New Phytologist, Wiley, 2010, 188 (1), pp.30-41. 10.1111/j.1469- 8137.2010.03303.x. hal-01806412 HAL Id: hal-01806412 https://hal.archives-ouvertes.fr/hal-01806412 Submitted on 16 Nov 2018 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés. Page 1 of 32 1 Diversity of Ectocarpus (Ectocarpales, Phaeophyceae) in Peru and 2 northern Chile, the area of origin of the genome-sequenced strain 3 4 Akira F. Peters1,2,3, Aaron D. Mann4, César A. Córdova5, Juliet Brodie6, Juan A. Correa4, 5 Declan C. Schroeder1 and J. Mark Cock3 6 For Peer Review 7 1 Marine Biological
    [Show full text]
  • Seaweed Species Diversity from Veraval and Sikka Coast, Gujarat, India
    Int.J.Curr.Microbiol.App.Sci (2020) 9(11): 3667-3675 International Journal of Current Microbiology and Applied Sciences ISSN: 2319-7706 Volume 9 Number 11 (2020) Journal homepage: http://www.ijcmas.com Original Research Article https://doi.org/10.20546/ijcmas.2020.911.441 Seaweed Species Diversity from Veraval and Sikka Coast, Gujarat, India Shivani Pathak*, A. J. Bhatt, U. G. Vandarvala and U. D. Vyas Department of Fisheries Resource Management, College of Fisheries Science, Veraval, Gujarat, India *Corresponding author ABSTRACT The aim of the present investigation focused on a different group of seaweeds observed K e yw or ds from Veraval and Sikka coasts, Gujarat from September 2019 to February 2020, to understand their seaweeds diversity. Seaweed diversity at Veraval and Sikka coasts has Seaweeds diversity, been studied for six months the using belt transect random sampling method. It was Veraval, Sikka observed that seaweeds were not found permanently during the study period but some species were observed only for short periods while other species occurred for a particular season. A total of 50 species of seaweeds were recorded in the present study, of which 17 Article Info species belong to green algae, 14 species belong to brown algae and 19 species of red Accepted: algae at Veraval and Sikka coasts. Rhodophyceae group was dominant among all the 24 October 2020 classes. There were variations in species of marine macroalgae between sites and Available Online: seasons.During the diversity survey, economically important species like Ulva lactuca, U. 10 November 2020 fasciata, Sargassum sp., and Caulerpa sp., were reported.
    [Show full text]
  • Ectocarpus: a Model Organism for the Brown Algae
    Downloaded from http://cshprotocols.cshlp.org/ on September 27, 2021 - Published by Cold Spring Harbor Laboratory Press Emerging Model Organism Ectocarpus: A Model Organism for the Brown Algae Susana M. Coelho,1,2,4 Delphine Scornet,1,2 Sylvie Rousvoal,1,2 Nick T. Peters,1,2 Laurence Dartevelle,1,2 Akira F. Peters,2,3 and J. Mark Cock1,2 1 UPMC Université Paris 06, The Marine Plants and Biomolecules Laboratory, UMR 7139, Station Biologique de Roscoff, BP74, 29682 Roscoff Cedex, France 2 CNRS, UMR 7139, Laboratoire International Associé Dispersal and Adaptation in Marine Species, Station Biologique de Roscoff, BP74, 29682 Roscoff Cedex, France 3 Bezhin Rosko, 29250 Santec, France The brown algae are an interesting group of organisms from several points of view. They are the domi- nant organisms in many coastal ecosystems, where they often form large, underwater forests. They also have an unusual evolutionary history, being members of the stramenopiles, which are very distantly related to well-studied animal and green plant models. As a consequence of this history, brown algae have evolved many novel features, for example in terms of their cell biology and metabolic path- ways. They are also one of only a small number of eukaryotic groups to have independently evolved complex multicellularity. Despite these interesting features, the brown algae have remained a relatively poorly studied group. This situation has started to change over the last few years, however, with the emergence of the filamentous brown alga Ectocarpus as a model system that is amenable to the genomic and genetic approaches that have proved to be so powerful in more classical model organisms such as Drosophila and Arabidopsis.
    [Show full text]
  • Oxygenated Acyclic Diterpenes with Anticancer Activity from the Irish Brown Seaweed Bifurcaria Bifurcata
    marine drugs Article Oxygenated Acyclic Diterpenes with Anticancer Activity from the Irish Brown Seaweed Bifurcaria bifurcata Vangelis Smyrniotopoulos 1, Christian Merten 2, Daria Firsova 1, Howard Fearnhead 3 and Deniz Tasdemir 1,4,5,* 1 School of Chemistry, National University of Ireland Galway, University Road, H91 TK33 Galway, Ireland; [email protected] (V.S.); dashafi[email protected] (D.F.) 2 Organische Chemie 2, Ruhr-Universität Bochum, Universitätsstraße 150, 44801 Bochum, Germany; [email protected] 3 Pharmacology and Therapeutics, School of Medicine, National University of Ireland Galway, University Road, H91 W2TY Galway, Ireland; [email protected] 4 GEOMAR Centre for Marine Biotechnology (GEOMAR-Biotech), Research Unit Marine Natural Product Chemistry, GEOMAR Helmholtz Centre for Ocean Research Kiel, Am Kiel-Kanal 44, 24106 Kiel, Germany 5 Faculty of Mathematics and Natural Sciences, Kiel University, Christian-Albrechts-Platz 4, 24118 Kiel, Germany * Correspondence: [email protected]; Tel.: +49-431-600-4430 Received: 28 October 2020; Accepted: 19 November 2020; Published: 23 November 2020 Abstract: Brown alga Bifurcaria bifurcata is a prolific source of bioactive acyclic (linear) diterpenes with high structural diversity. In the continuation of our investigations on Irish brown algae, we undertook an in-depth chemical study on the n-hexanes and chloroform subextracts of B. bifurcata that led to isolation of six new (1–6) and two known (7–8) acyclic diterpenes. Chemical structures of the compounds were elucidated by a combination of 1D and 2D NMR, HRMS, FT-IR, [α]D and vibrational circular dichroism (VCD) spectroscopy. Compounds 1–8, as well as three additional linear diterpenes (9–11), which we isolated from the same seaweed before, were tested against the human breast cancer cell line (MDA-MB-231).
    [Show full text]