Focus on ETHICS Ffaallll 2006 • Volume 27:4
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Citizen Cyborg.” Citizen a Groundbreaking Work of Social Commentary, Citizen Cyborg Artificial Intelligence, Nanotechnology, and Genetic Engineering —DR
hughes (continued from front flap) $26.95 US ADVANCE PRAISE FOR ARTIFICIAL INTELLIGENCE NANOTECHNOLOGY GENETIC ENGINEERING MEDICAL ETHICS INVITRO FERTILIZATION STEM-CELL RESEARCH $37.95 CAN citizen LIFE EXTENSION GENETIC PATENTS HUMAN GENETIC ENGINEERING CLONING SEX SELECTION ASSISTED SUICIDE UNIVERSAL HEALTHCARE human genetic engineering, sex selection, drugs, and assisted In the next fifty years, life spans will extend well beyond a century. suicide—and concludes with a concrete political agenda for pro- cyborg Our senses and cognition will be enhanced. We will have greater technology progressives, including expanding and deepening control over our emotions and memory. Our bodies and brains “A challenging and provocative look at the intersection of human self-modification and human rights, reforming genetic patent laws, and providing SOCIETIES MUST RESPOND TO THE REDESIGNED HUMAN OF FUTURE WHY DEMOCRATIC will be surrounded by and merged with computer power. The limits political governance. Everyone wondering how society will be able to handle the coming citizen everyone with healthcare and a basic guaranteed income. of the human body will be transcended, as technologies such as possibilities of A.I. and genomics should read Citizen Cyborg.” citizen A groundbreaking work of social commentary, Citizen Cyborg artificial intelligence, nanotechnology, and genetic engineering —DR. GREGORY STOCK, author of Redesigning Humans illuminates the technologies that are pushing the boundaries of converge and accelerate. With them, we will redesign ourselves and humanness—and the debate that may determine the future of the our children into varieties of posthumanity. “A powerful indictment of the anti-rationalist attitudes that are dominating our national human race itself. -
Selection of Cryoprotectant in Lyophilization of Progesterone-Loaded Stearic Acid Solid Lipid Nanoparticles
pharmaceutics Article Selection of Cryoprotectant in Lyophilization of Progesterone-Loaded Stearic Acid Solid Lipid Nanoparticles Timothy M. Amis, Jwala Renukuntla, Pradeep Kumar Bolla and Bradley A. Clark * Department of Basic Pharmaceutical Sciences, Fred Wilson School of Pharmacy, High Point University, High Point, NC 27268, USA; [email protected] (T.M.A.); [email protected] (J.R.); [email protected] (P.K.B.) * Correspondence: [email protected]; Tel.: +1-336-841-9665 Received: 18 August 2020; Accepted: 16 September 2020; Published: 19 September 2020 Abstract: Cryoprotectants are often required in lyophilization to reduce or eliminate agglomeration of solute or suspended materials. The aim of this study was to select a cryoprotecting agent and optimize its concentration in a solid lipid nanoparticle formulation. Progesterone-loaded stearic acid solid lipid nanoparticles (SA-P SLNs) were prepared by hot homogenization with high speed mixing and sonication. The stearic acid content was 4.6% w/w and progesterone was 0.46% w/w of the initial formulation. Multiple surfactants were evaluated, and a lecithin and sodium taurocholate system was chosen. Three concentrations of surfactant were then evaluated, and a concentration of 2% w/w was chosen based on particle size, polydispersity, and zeta potential. Agglomeration of SA-P SLNs after lyophilization was observed as measured by increased particle size. Dextran, glycine, mannitol, polyvinylpyrrolidone (PVP), sorbitol, and trehalose were evaluated as cryoprotectants by both an initial freeze–thaw analysis and after lyophilization. Once selected as the cryoprotectant, trehalose was evaluated at 5%, 10%, 15%, and 20% for optimal concentration, with 20% trehalose being finally selected as the level of choice. -
Cryoprotectant Production Capacity of the Freeze-Tolerant Wood Frog, Rana Sylvatica
71 Cryoprotectant production capacity of the freeze-tolerant wood frog, Rana sylvatica JoN P. Cosr,cNzo AND Rlcnano E. LsE, Jn. Department of Zoology, Miami University, Oxford, OH 45056, U.S.A. ReceivedJune 8. 1992 AcceptedAugust 17, 1992 CosmNzo, J. P., and LBp, R. E., Jn. 1993. Cryoprotectantproduction capacityof the freeze-tolerantwood frog, Rana sylvatica.Can. J. Zool. 7I: 7l-75. Freezingsurvival of the wood frog (Rana sylvatica)is enhancedby the synthesisof the cryoprotectantglucose, via liver glycogenolysis.Because the quantityof glucosemobilized during freezingbears significantly on the limit of freezetolerance, we investigated the relationship between the quantity of liver glycogen and the capacity for cryoprotectant synthesis. We successfullyaugmented natural levels of liver glycogenby injecting cold-conditionedwood frogs with glucose.Groups of 8 frogs having mean liver glycogenconcentrations of 554 + 57 (SE), 940 + 57, and 1264 + 66 pmollg catabolized98.7, 83.4, and 52.8%, respectively,of their glycogenreserves during 24 h of freezingto -2.5'C. Glucoseconcentrations con- comitantly increased,reaching 2l + 3, 102 + 23, and 119 + 14 pmollg, respectively,in the liver, and 15 * 3,42 + 5, and 6l * 5 pcmol/ml-, respectively, in the blood. Becausethe capacity for cryoprotectant synthesisdepends on the amount of liver glycogen,the greatestrisk of freezinginjury likely occursduring spring,when glycogenreserves are minimal. Non- glucoseosmolites were important in the wood frog's cryoprotectantsystem, especially in frogs having low glycogenlevels. Presumably the natural variation in cryoprotectant synthesis capacity among individuals and populations of R. sylvatica chiefly reflectsdifferences in glycogenreserves; however, environmental,physiological, and geneticfactors likely are also involved. CosmNzo, J. P., et LEs, R. E., Jn. -
Sperm Cryopreservation: a Review on Current Molecular Cryobiology and Advanced Approaches
1 RBMO VOLUME 00 ISSUE 0 2018 REVIEW Sperm cryopreservation: A review on current molecular cryobiology and advanced approaches BIOGRAPHY Abdolhossein Shahverdi is Professor of Embryology and scientific director of the Sperm Biology Group at the Royan Institute in Tehran. His main research interests are fertility preservation, reproductive epigenetic and germ cell biology. With over 20 years’ experience in reproductive biology, he has published more than 120 international scientific papers. Maryam Hezavehei1,2, Mohsen Sharafi3,*, Homa Mohseni Kouchesfahani2, Ralf Henkel4, Ashok Agarwal5, Vahid Esmaeili1, Abdolhossein Shahverdi1,* KEY MESSAGE Understanding the new aspects of sperm cryobiology, such as epigenetic and proteomic modulation, as well as novel techniques, is essential for clinical applications and the improvement of ART protocols. Long-term follow-up studies on the resultant offspring obtained from cryopreserved spermatozoa is recommended for future studies. ABSTRACT The cryopreservation of spermatozoa was introduced in the 1960s as a route to fertility preservation. Despite the extensive progress that has been made in this field, the biological and biochemical mechanisms involved in cryopreservation have not been thoroughly elucidated to date. Various factors during the freezing process, including sudden temperature changes, ice formation and osmotic stress, have been proposed as reasons for poor sperm quality post-thaw. Little is known regarding the new aspects of sperm cryobiology, such as epigenetic and proteomic modulation of sperm and trans-generational effects of sperm freezing. This article reviews recent reports on molecular and cellular modifications of spermatozoa during cryopreservation in order to collate the existing understanding in this field. The aim is to discuss current freezing techniques and novel strategies that have been developed for sperm protection against cryo-damage, as well as evaluating the probable effects of sperm freezing on offspring health. -
Effects of Sample Treatments on Genome Recovery Via Single-Cell Genomics
The ISME Journal (2014) 8, 2546–2549 & 2014 International Society for Microbial Ecology All rights reserved 1751-7362/14 www.nature.com/ismej SHORT COMMUNICATION Effects of sample treatments on genome recovery via single-cell genomics Scott Clingenpeel1, Patrick Schwientek1, Philip Hugenholtz2 and Tanja Woyke1 1DOE Joint Genome Institute, Walnut Creek, CA, USA and 2Australian Centre for Ecogenomics, School of Chemistry and Molecular Biosciences, University of Queensland, Brisbane, Queensland, Australia Single-cell genomics is a powerful tool for accessing genetic information from uncultivated microorganisms. Methods of handling samples before single-cell genomic amplification may affect the quality of the genomes obtained. Using three bacterial strains we show that, compared to cryopreservation, lower-quality single-cell genomes are recovered when the sample is preserved in ethanol or if the sample undergoes fluorescence in situ hybridization, while sample preservation in paraformaldehyde renders it completely unsuitable for sequencing. The ISME Journal (2014) 8, 2546–2549; doi:10.1038/ismej.2014.92; published online 13 June 2014 Relatively little is known about the functioning of genomes from single cells. Three methods of sample complex microbial communities, largely due to the preservation were tested: cryopreservation with difficulty in culturing most microbes (Rappe and 20% glycerol as a cryoprotectant, preservation in Giovannoni, 2003). Although metagenomics can 70% ethanol, and preservation in 4% paraformalde- provide information on the genetic capabilities of hyde. Because paraformaldehyde treatment causes the entire community, it is difficult to connect crosslinks between nucleic acids and proteins, we predicted gene functions to specific organisms using tested cells exposed to 4% paraformaldehyde with metagenomics (Morales and Holben, 2011). -
Physical and Chemical Changes in Stabilized Mince from Pacific Whiting During Frozen Storage
AN ABSTRACT OF THE THESIS OF Edda Magnusdottir for the degree of Master of Science in Food Science & Technology presented on April 28, 1995. Title: Physical and Chemical Changes in Stabilized Mince from Pacific Whiting During Frozen Storage. Abstract approved: - L Michael T. Morrissey Cryoprotection in stabilized mince from Pacific whiting (Merluccius productus) was investigated by monitoring changes in physical and chemical properties during 32 weeks of frozen storage. The effects of 4 different cryoprotectants were evaluated by torsion test, color analysis, extractability of salt soluble proteins, and formation of dimethylamine (DMA) and 2-thiobarbituric acid (TBA). The quality of the stabilized mince was significantly higher than the control (mince without cryoprotectants) when compared by shear strain, salt soluble proteins, and DMA. The results show that the functionality of the proteins in the mince can be protected by using cryoprotectants with Polydextrose® being the most effective of the 4 tested. The effect of food-grade protease inhibitors on the gel-forming characteristics of Pacific whiting mince was also investigated. Four levels (1, 2, 3, and 4%) of different protease inhibitors (beef plasma protein, whey protein concentrate, egg white liquid, and egg white powder) were added to the stabilized mince before heating and effects on texture and color were evaluated. Shear strain was significantly increased by increasing the level of inhibitors. Beef plasma protein was most effective and presented significantly higher strain than the other inhibitors tested. Due to higher concentration of proteolytic enzymes in the mince, an increased amount of protease inhibitors is needed compared to surimi to prevent proteolysis during heating. -
Cryopreservation Page 3
2nd quarter 2010 • Volume 31:2 funding Your Cryopreservation page 3 Death of Robert Prehoda Page 7 Member Profile: Mark Plus page 8 Non-existence ISSN 1054-4305 is Hard to Do page 14 $9.95 Improve Your Odds of a Good Cryopreservation You have your cryonics funding and contracts in place but have you considered other steps you can take to prevent problems down the road? Keep Alcor up-to-date about personal and medical changes. Update your Alcor paperwork to reflect your current wishes. Execute a cryonics-friendly Living Will and Durable Power of Attorney for Health Care. Wear your bracelet and talk to your friends and family about your desire to be cryopreserved. Ask your relatives to sign Affidavits stating that they will not interfere with your cryopreservation. Attend local cryonics meetings or start a local group yourself. Contribute to Alcor’s operations and research. Contact Alcor (1-877-462-5267) and let us know how we can assist you. Alcor Life Extension Foundation is on Connect with Alcor members and supporters on our official Facebook page: http://www.facebook.com/alcor.life.extension.foundation Become a fan and encourage interested friends, family members, and colleagues to support us too. 2ND QUARTER 2010 • VOLUME 31:2 2nd quarter 2010 • Volume 31:2 Contents COVER STORY: PAGE 3 funding Your Cryopreservation Without bequests and page 3 donations Alcor’s revenue falls 11 Book Review: The short of covering its operating Rational Optimist: How expenses. This means that Prosperity Evolves Alcor should further cut costs Former Alcor President or increase revenue. -
Kinetic Vitrification of Spermatozoa of Vertebrates: What Can We Learn from Nature?
1 Kinetic Vitrification of Spermatozoa of Vertebrates: What Can We Learn from Nature? I.I. Katkov** et al.* CELLTRONIX and Sanford-Burnham Institute for Medical Research, San Diego, California, USA Dedicated to the memory of Father Basile J. Luyet (1897-1974) 1. Introduction This as well as two other related Chapters, by Isachenko et al. and Moskovtsev et al., open this Book neither accidentally nor by the Editor’s preferences to his friends and collaborators; the reasons, in fact, lie quite deeper: Why sperm? Cryobiology had actually started from freezing sperm. We will skip all those very early anecdotes but should mention the Spallanzani attempt to freeze frog semen in the 18th century [Spallanzani, 1780]. Cryobiology as a science started with revolutionizing work of Father Luyet and other scientists of the late 1930’s and 1940’s, who we can collectively call “the pioneers of the cryobiological frontiers” (see the following sub-Chapter). There were several reasons why sperm was chosen, which included easiness in obtaining the samples, clear evidence of viability (moving – not moving, though later it was figured that everything was not so easy in this sophisticated living “cruise missile”), and importance for the farming industry with the emergence of systematic selective breeding (especially in cattle) with a powerful tool – artificial insemination (AI). AI started with the revolutionary work of W. Heape, I.I. Ivanov and other scientists at the dawn of the 20th century and was further developed by V.K. Milovanov in the 1930’s as a viable breeding technology (see [Foote, * V.F. Bolyukh2, O.A. -
A Review and Application of Cryoprotectant: the Science of Cryonics
PharmaTutor PRINT ISSN: 2394-6679 | E-ISSN: 2347-7881 12 A Review and Application of Cryoprotectant: The Science of Cryonics Ankit J. Joshi Department of Pharmaceutics & Pharmaceutical Technology, S. K. Patel College of Pharmaceutical Education and Research, Ganpat University, Kherva, Gujarat. [email protected] ABSTRACT The preservation of cells, tissues and organs by cryopreservation is promising technology now days and Low temperature technology has progressed in the field of tissue engineering, food preservation, fertility preservation, making disease resistant breeds since the early years to occupy a central role in this technology. Cryopreservation is important technology in every field like in organ cryopreservation, food cryopreservation, human cryopreservation, seeds cryopreservation, protein cryopreservation and pharmaceuticals. Cryobiologists will be required to collaborate with new physical and molecular sciences to meet this challenge. How cryoprotectants work is a mystery to most people. In fact, how they work was even a mystery to science until just a few decades ago. This article will explain in basic terms how cryoprotectants protect cells from damage caused by ice crystals, and with some of the advances. Key Words: Cryopreservation, Cryoprotectants, vitrification, Penetrating & non-penetrating cryoprotectant. INTRODUCTION A cryoprotectant is a substance used to protect Types of cryopreservation [1-4] biological tissue from freezing damage (i.e. that due (1) Isochoric cryopreservation to ice formation). Cryopreservation is a process Isochoric (constant volume) cryopreservation is where cells or tissues are preserved by cooling to low referred for biological material at low temperature. sub-zero temperatures, such as 77 K or -196°C (the In isochoric cryopreservation, the solution boiling point of liquid nitrogen). -
Preparing Samples for Pacbio Whole Genome Sequencing for De Novo
Technical Note Sample Prep Technical Note Sample Prep Preparing Samples for PacBio® Whole Genome Sequencing for de novo Assembly: Collection and Storage Introduction Single Molecule, Real-Time (SMRT®) Sequencing uses the natural process of DNA replication to sequence long fragments of native DNA. As such, starting with high-quality, high molecular weight (HMW) genomic DNA (gDNA) will result in better sequencing performance across difficult to sequence regions of the genome. To obtain the highest quality, long DNA it is important to start with sample types compatible with HMW DNA extraction methods. This technical note is intended to give general guidance on sample collection, preparation, and storage across a range of commonly encountered sample types used for SMRT Sequencing whole genome projects. It is important to note that all samples and projects are unique and may not be comprehensively addressed in this document. Topics Covered • Sample and storage recommendations for: - Vertebrates - mammals, birds, fish, amphibians, reptiles - Invertebrates - marine, terrestrial - Arthropods - insects, crustaceans - Fungi - microorganisms, mushrooms, algae* - Plants - broad leaf plants, grasses *Algae is included with fungi due to similar growth and storage conditions • Additional considerations for planning HMW DNA isolation Sample and Storage Recommendations Vertebrates When sampling from vertebrates it is recommended to use a single individual. Sample Type Sample Storage Cell-dense tissue Fresh (within ~24 hours of collection and kept at 4°C) or flash frozen with liquid nitrogen 1 (brain, kidney, and stored at -80°C. muscle, etc.) Whole blood (if Collected in tube with anticoagulant such as EDTA, blood sample will be viable at 4°C for 24-72 hours 2 red blood cells are after collection. -
Technological Enhancement and Criminal Responsibility1
THIS VERSION DOES NOT CONTAIN PAGE NUMBERS. PLEASE CONSULT THE PRINT OR ONLINE DATABASE VERSIONS FOR PROPER CITATION INFORMATION. ARTICLE HUMANS AND HUMANS+: TECHNOLOGICAL ENHANCEMENT AND CRIMINAL RESPONSIBILITY1 SUSAN W. BRENNER2 I. INTRODUCTION [G]radually, the truth dawned on me: that Man had not remained one species . .3 It has been approximately 30,000 years since our species—Homo sapiens sapiens—shared the earth with another member of the genus Homo.4 I note 1 It was only after I decided to use Humans+ as part of the title for this article that I discovered the very similar device used by the transhumanist organization, Humanity+. See Mission, HUMANITY PLUS, http://humanityplus.org/about/mission/ (last visited Feb. 25, 2013). 2 NCR Distinguished Professor of Law & Technology, University of Dayton School of Law, Dayton, Ohio, USA. Email: [email protected]. 3 H.G. WELLS, THE TIME MACHINE 59 (Frank D. McConnell ed. 1977). 4 See GRAHAM CONNAH, FORGOTTEN AFRICA: AN INTRODUCTION TO ITS ARCHAEOLOGY 7–16 (2004) (supporting emergence of genus Homo in Africa); CHRIS GOSDEN, PREHISTORY: A VERY SHORT INTRODUCTION xiv–xv, 39–42 (2003) (summarizing rise of Homo sapiens sapiens). “Taxonomy,” or “the study of classification,” involves the process of sorting plants and animals into categories, including species. STEPHEN JAY GOULD, EVER SINCE DARWIN: REFLECTIONS IN NATURAL HISTORY 231 (1977). The species Homo sapiens includes Homo sapiens sapiens (modern humans) as well as several “archaic” members of the species. See, e.g., William Howells, Getting Here: The Story of Human Evolution 122– 23 (Washington, DC: Compass Press 1993). See also R.A. -
Optimization of Sperm Cryopreservation Protocol for Peregrine Falcon (Falco Peregrinus)
animals Article Optimization of Sperm Cryopreservation Protocol for Peregrine Falcon (Falco peregrinus) Beatriz Cardoso 1, Irene Sánchez-Ajofrín 1,* , Cristina Castaño 2, Olga García-Álvarez 1 , Milagros Cristina Esteso 2, Alejandro Maroto-Morales 1, María Iniesta-Cuerda 1 , José Julián Garde 1 , Julián Santiago-Moreno 2 and Ana Josefa Soler 1 1 SaBio IREC (CSIC-UCLM-JCCM), ETSIAM, 02071 Albacete, Spain; [email protected] (B.C.); [email protected] (O.G.-Á.); [email protected] (A.M.-M.); [email protected] (M.I.-C.); [email protected] (J.J.G.); [email protected] (A.J.S.) 2 Department of Animal Reproduction, INIA, 28040 Madrid, Spain; [email protected] (C.C.); [email protected] (M.C.E.); [email protected] (J.S.-M.) * Correspondence: [email protected] Received: 25 March 2020; Accepted: 14 April 2020; Published: 16 April 2020 Simple Summary: The process of semen cryopreservation can have multiple advantages in an ex situ conservation program. However, there is a necessity to adapt the protocol to the specificity of each species. With that in mind, we aimed to optimize the sperm freezing/thawing process and study the effect of different cryoprotectants in the peregrine falcon. Abstract: Sperm cryopreservation is a complex process that needs to be adapted to wild and domestic avian species to ensure proper efficiency. Because of its accessibility, the peregrine falcon may be used as a good model for studying other raptor species. To find the most optimal cryopreservation protocol for peregrine falcon ejaculates, sperm parameters such as motility, viability, DNA fragmentation, acrosome integrity, and mitochondrial activity were analyzed under different conditions by varying the freezing method (slow freezing in straws vs.