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Supplementary Table 1: List of the Lung Cancer Cell Lines Used in the Present Study for Determining the Status of PARD3

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Supplementary Table 1: List of the Lung Cancer Cell Lines Used in the Present Study for Determining the Status of PARD3 Supplementary Table 1: List of the lung cancer cell lines used in the present study for determining the status of PARD3. The histopathological type of each cell line is also indicated. CELL LINE ID HISTOPATHOLOGICAL TYPE A427 ADENOCARCINOMA Calu3 ADENOCARCINOMA DMS-273 SMALL CELL LUNG CANCER EBC1 SQUAMOUS CELL CARCINOMA H1155 NON-SMALL CELL LUNG CANCER H128 SMALL CELL LUNG CANCER H1299 NON-SMALL CELL LUNG CANCER H1395 ADENOCARCINOMA H1437 ADENOCARCINOMA H157 SQUAMOUS CELL CARCINOMA H1573 ADENOCARCINOMA H1650 ADENOCARCINOMA H1703 ADENOCARCINOMA H1770 NEUROENDOCRINE H1975 ADENOCARCINOMA H1993 ADENOCARCINOMA H2029 SMALL CELL LUNG CANCER H2107 SMALL CELL LUNG CANCER H2126 ADENOCARCINOMA H2141 SMALL CELL LUNG CANCER H2170 SQUAMOUS CELL CARCINOMA H2171 SMALL CELL LUNG CANCER H2195 SMALL CELL LUNG CANCER H23 ADENOCARCINOMA H345 SMALL CELL LUNG CANCER H441 ADENOCARCINOMA H446 SMALL CELL LUNG CANCER H460 LARGE CELL CARCINOMA H520 SQUAMOUS CELL CARCINOMA H522 ADENOCARCINOMA H596 SQUAMOUS CELL CARCINOMA H69 SMALL CELL LUNG CANCER H82 SMALL CELL LUNG CANCER H889 SMALL CELL LUNG CANCER HCC15 SQUAMOUS CELL CARCINOMA HCC366 NON-SMALL CELL LUNG CANCER HCC95 SQUAMOUS CELL CARCINOMA HTB58 SQUAMOUS CELL CARCINOMA LC1SQ SQUAMOUS CELL CARCINOMA LK-2 SQUAMOUS CELL CARCINOMA LU-134 SMALL CELL LUNG CANCER LU-135 SMALL CELL LUNG CANCER LU-139 SMALL CELL LUNG CANCER LU-65 LARGE CELL CARCINOMA LUDLU-1 SQUAMOUS CELL CARCINOMA PC10 SQUAMOUS CELL CARCINOMA PC-14 SQUAMOUS CELL CARCINOMA PC9 ADENOCARCINOMA RERF-LCMS ADENOCARCINOMA SKMES1 SQUAMOUS CELL CARCINOMA SQ1 SQUAMOUS CELL CARCINOMA Supplementary Table 2 DNA 5'-3' PARD3_E1F TCCTCGGTCCCGGCC PARD3_E1R CCAGGAAGCGCCATATTGATC PARD3_E2F ATGGGCAGGAAGTTATCACG PARD3_E2R CCTAGTGTTAAGTCCCAGGTATCAAG PARD3_E3F TTTGAATGTGAAATGTACGACTTAG PARD3_E3R TTCCATTTACAGGGTCATGG PARD3_E4F TTTAGGATTTCCCTACGTTCTCC PARD3_E4R CAATGGTTTACCCAAGACACG PARD3_E5F TCGGCACATGGTTATAATTCC PARD3_E5R CCAGAAATATCCTGCATTTATGTTAG PARD3_E6F CAAGATCATAATGCCTATTAGACTTCC PARD3_E6R TCCTCATGCCATATGCTTTC PARD3_E7F TGTGTGTCTGTGTTGTTGCAG PARD3_E7R TCAACACCACTCTCTATCTGAGC PARD3_E8F TCATTTTAACATGCTCCTTTGTAAG PARD3_E8R GGCAGAAAATATTCCTCTCCAG PARD3_E9F AACAGCCTGTCAGTTCTTATTGC PARD3_E9R AGGGTGCTTTGGGTTAGTAGG PARD3_E10F GAAATGTGGACCATTGTGAAAG PARD3_E10R CGCATACTTCCGCTAAAATG PARD3_E11F GGCTAGTCCTAGAGTGTGTGTGTG PARD3_E11R CTCAATAAACATTTGCCATCAGG PARD3_E12F ACAGAGCATTATGTCAGCCATC PARD3_E12R GACCACTTTGGCATTTAATAAACC PARD3_E13F TTTCATGGATCTTGCTGTGG PARD3_E13R CGTGACACATCACTGATACACC PARD3_E14F CGTTCAATTGGGCATAATCC PARD3_E14R AAGGTCCTGGAACCTAATACCC PARD3_E15F TCGGGCAAGTTGTTATAATTCTC PARD3_E15R TTTGGTTGCTTCGTCATGTTAC PARD3_E16F AGACTCGAGGTCTCTGTTTGAC PARD3_E16R TTAAATTTACAGAGTTCGGGTATAATC PARD3_E17F CACTTGCAGGTATGTATGAACCTC PARD3_E17R GACATCTGCTTGGGACCATC PARD3_E18F ATGACTGGCATATTGCATGG PARD3_E18R GGCATATGATTGGCAGCTC PARD3_E19F TGAGCAAATAATTGTTATGTACCTG PARD3_E19R GAGTAAAGGGCAAATAATATCGTC PARD3_E20F GCATTGCAGTCCATGTTGC PARD3_E20R TTCAGCCTCCCAAGTAGCTG PARD3_E21F ACTGCAGAAGACACCTGATCC PARD3_E21R CCATCAATATTTGGGTTGATACAG PARD3_E22F CCACTGATCTTCTATAGCTGCAAG PARD3_E22R AAGGCAATTCTGGTTTGAGG PARD3_E23F CAGCAAGTTGCCATGTTTTG PARD3_E23R TCGTTTCATAGAGCATTGAGGTC PARD3_E24F CATGCCAAAGCCAAAATCAC PARD3_E24R CGGGACATTTATTGGTTCCTC PARD3_E25F GCATATTCCATCCCATCCTG PARD3_E25R GAAACAGACACTTGAGACATAGTGG cloning 5'-3' PARD3_F ATAAGCTTAGCATGAAAGTGACCGTGTGCTTCGGACGGAC PARD3_R ATGCGGCCGCGGAATAGAAGGGCCTCCCTTTCTCAGGAG cDNA 5'-3' PARD3_E4F TTGGCCTCTCCACTTCTGTC PARD3_E7R TCGTTGGGGACTTCTACGAG PARD3_E11F GTGGGCAAATCCCAAGAGG PARD3_E13R GACAAAGATTCCCAAATCTGC PARD3_E15F AGCGTTGGATGATAGAGAACG PARD3_E19R TCTTCAGACCCAGGGAAGG PARD3_E20F TGAATGGAAACCAAGAGAAAGG PARD3_E22R GGAACCTTCATAAGAAGAAACTCC Supplementary Table 3: List of the different single nucleotide polymorphisms (SNPs) and variants of unknown biological significance identified in the lung cancer cell lines and in the lung primary tumors tested in the present study. protein Frequency cDNA nucleotide change change SNP (%) c.258C>T p.H86H rs112281243 0.69 c.321G>C p.E107D rs1436731 1.38 c.339C>G p.V113V rs41276096 0.69 c.581A>G p.K194R no reference 0.69 c.582+36C>G NO CHANGE rs150161748 0.69 c.714+26C>T NO CHANGE rs1370084 0.69 c.714+79A>G NO CHANGE rs982687 6.21 c.714+117C>T NO CHANGE rs74134137 0.69 c.861T>C NO CHANGE rs2796011 0.69 c.891-95C>T NO CHANGE rs117300707 2.76 c.1045C>T p.R349C rs201536019 0,69 c.1539+31G>A NO CHANGE rs10740890 72.41 c.1669-91C>G NO CHANGE rs10763987 35.86 c.1749C>G p.T583T rs61735565 6.90 c.1896+116C>T NO CHANGE rs78468914 0.69 c.2227+64G>A NO CHANGE rs7077783 28.97 c.2228-40G>T NO CHANGE no reference 0.69 c.2402G>A p.S801N rs118153230 0.69 c.2569+10A>G NO CHANGE rs58731858 0.69 c.2604C>G N868E no reference 0,69 2614+26 C>T NO CHANGE TMP_ESP_10_34625101 0.69 c.2667G>A p.S889S rs.3781128 71.72 c.2843+99T>G NO CHANGE rs10740885 58.62 c.2810C>T p.A937V rs16935326 2.07 c.2615-62A>G NO CHANGE rs11009737 1.38 c.3185+12T>A NO CHANGE no reference 1.38 3186-12C>T NO CHANGE rs41276094 4.14 c.3549+23C>T NO CHANGE rs116214419 0.69 c.3550-77T>G NO CHANGE rs3004928 71.72 c.3582A>G p.R1191R rs11009651 26.21 c.3550-133G>A NO CHANGE rs143734834 0.69 c.3569C>T p.T1190M rs61735568 0.69 c.3678-28A>G NO CHANGE no reference 0.69 c.3765G>A p.E1255E rs144844232 0.69 c.4019C>T p.A1340V rs144433754 0.69 c.*29C>T UNKNOWN rs150058603 0.69 SUPPLEMENTARY TABLE 4 Tumor ID TYPE AGE GENDER DIFFERENTIATION TNM STAGE METASTASIS SURVIVAL PARD3 GENE STATUS PAR3 IHC pY-STAT3 IHC NI_NO INFORMATION AVAILABLE 100 SCC 77 M MODERATE NI NI NI NI wt 11 104 SCC 58 M POOR T3N0 IIB NO 48+ wt 01 108 SCC 73 M MODERATE T1NX ? NO 48+ wt 10 110 SCC 43 M MODERATE T2NX IV YES 10 wt 11 114 SCC 70 M MODERATE T3NX IV YES 51 c.121-?_2067+?del; p.D41_E689del 0NI 116 SCC 52 M POOR T2N0 IB NO 55 wt 0NI 120 SCC 69 M POOR T2N0 IB NO 13+ wt 21 139 SCC 60 M MODERATE T2N2 IIIA NO 47+ wt 11 141 SCC 77 M MODERATE T2N0MX IB NI NI c.121-2A>G; p.D41_R74del 1NI 143 SCC 80 M MODERATE T2N1 IIB YES 13 wt 11 149 SCC 80 M POOR T3N0MX IIB NO 48+ wt 21 156 SCC 67 M POOR T2N0 IB NO 59+ wt 11 159 SCC 70 M WELL T1N2 IIIA NO 47+ wt 00 160 SCC 73 M WELL T2N0 IB NI 42 wt 0NI 162 SCC 46 M WELL T1N1 IIA NO 37+ wt 01 164 SCC 61 M POOR T2N1 IIB NI wt 1NI 167 SCC 75 M WELL T4N0 IIIB NO 53+ wt 01 168 SCC 69 M WELL T2N0 IB NO 13+ wt 10 170 SCC 71 M WELL T2N0 IB NO 48+ wt 11 172 SCC 66 M NI T3 IIB NI NI wt 10 181 SCC 71 M POOR T2N1 IIB NI NI c.404-?_582+?del; No protein 10 184 SCC 76 M NI T2 IB NI NI wt 11 186 SCC NI NI NI T1 IA NI NI wt 00 189 SCC 66 M WELL T1 IA NI NI wt NI 0 190 SCC 71 M WELL T2 IB NI NI wt 10 192 SCC 57 M POOR T1 IA NI NI wt 00 193 SCC 64 M POOR T1 IA NI NI wt NI NI 194 SCC 78 M POOR T1 IA NI NI wt NI NI 196 SCC 65 M POOR T1 IA NI NI wt 01 197 SCC 68 M WELL T2 IB NI NI wt NI NI 199 SCC 72 M POOR T2 IB NI NI wt 10 200 SCC 77 M MODERATE T2 IB NI NI c.1492insG; p.A498Gfs*9 00 202 SCC 69 M NI T2 IB NI NI wt 11 203 SCC 63 M NI T3 IIB NI NI wt 21 205 SCC 72 M WELL T2 IB NI NI wt 10 207 SCC 65 M NI T2 IB NI NI wt 31 210 SCC 69 M POOR T3 IIB NI NI wt 20 212 SCC 61 M POOR T2 IB NI NI wt 10 213 SCC 60 M WELL T2 IB NI NI wt 10 217 SCC 66 M POOR T3N1 IIIA NI NI wt 10 218 SCC 68 M WELL T1 IA NI NI wt 11 224 SCC 56 M WELL T2N0 IB NO 48+ wt 30 229 SCC 67 M NI T1NX NI NI NI wt 20 237 SCC 61 M WELL T2N0 IB NO 52+ wt NI NI 238 SCC 69 M WELL T2 IB NI NI wt 10 239 SCC 69 M WELL T2 IB NI NI wt 20 240 SCC 59 M WELL T1N0 IA NI 47 wt NI NI 251 SCC NI NI NI NI NI NI NI wt 31 252 SCC 64 M WELL T1N0 IA NO 48+ wt 20 254 SCC 74 M WELL T2 IB NI NI wt 11 256 SCC 47 M MODERATE T2N0 IB NO 47+ wt 11 264 SCC 66 M POOR T2N0 IB NI 19 wt 20 267 SCC 61 M MODERATE T1N0 IA NO 48+ wt 10 269 SCC 79 M MODERATE T2N0 IB NI 28 c.1034G>A; p.R345H 10 273 SCC 74 M POOR T3 IIB NI NI c.3129A>G; p.I1043M NI NI 276 SCC 73 M WELL T3N0 IIB NO 42+ wt 30 360 SCC 66 M POOR T1N0 IA NO 62+ wt 20 362 SCC 69 M POOR T2N1 IIB NI 1 wt 20 368 SCC 63 M WELL T1N0 IA NI 1 wt 2NI 370 SCC 65 M POOR T2 NI NI NI wt 2NI 374 SCC 54 M WELL T1N2 IIIA NO 39+ wt 2NI 388 SCC 76 M WELL T2N0 IB NI 34 wt 1NI 471 SCC 74 M WELL T2N2MX NI NI NI wt NI NI 474 SCC 59 M WELL NI NI NI NI wt NI NI 478 SCC 64 M WELL T1N0MX NI NI NI wt NI NI 482 SCC 71 M WELL NI NI NI NI wt NI NI 490 SCC 55 M WELL T2NOMX NI NI NI wt NI NI 495 SCC 62 M WELL NI NI NI NI wt NI NI 617 SCC 63 M POOR T3N0 IIB NI NI wt NI NI 619 SCC NI NI MODERATE T2N0 IB NI NI wt NI NI 847 SCC 72 M MODERATE T3N0 IIB NI 2 wt NI NI 857 SCC 79 F MODERATE T1N2 IIIA NI 15 wt NI NI 901 SCC 70 M MODERATE T1N0 IA NO 17+ wt NI NI 915 SCC 82 M MODERATE T2N0 1B NO 20+ wt NI NI 943 SCC 74 F MODERATE T2N0 IB NI 8 wt NI NI 958 SCC 72 M MODERATE T1N0 IA NO 11+ wt NI NI 961 SCC 72 F MODERATE T1N0 IA NO 19+ wt NI NI 975 SCC 65 F POOR T2N2 IIIA NI 11 wt NI NI 978 SCC 72 M MODERATE T2N2 IIIA NO 15+ wt NI NI 1101 SCC 63 M MODERATE T1N0 IA NO 12+ wt NI NI 1375 SCC 75 F POOR T2N0 IB NO 6+ wt NI NI 1529 SCC 69 M MODERATE T2N0 IB NI NI c.404-?_3549+?del; No protein NI NI 1631 SCC 76 M MODERATE T1N0 IA NI NI wt NI NI 1642 SCC 54 M MODERATE T1N0 IA NI NI wt NI NI 1681 SCC NI NI NI NI NI NI NI c.223-?_3549+?del; No protein NI NI 1928 SCC NI NI NI NI NI NI NI wt NI NI 1953 SCC NI NI NI NI NI NI NI wt NI NI 2022 SCC NI NI NI NI NI NI NI wt NI NI T1 SCC 68 F NI T2N1M0 IIb NO 23 wt NI NI T10 SCC 73 M MODERATE T1N0M0 Ia YES 11 wt NI NI T11 SCC 72 M MODERATE T3N0M0 IIb YES 18 wt NI NI T12 SCC 56 M MODERATE T2N2M0 IIIa NO 19+ wt NI NI T13 SCC 75 M MODERATE T2N0M0 Ib NO 11 wt NI NI T14 SCC 76 M MODERATE T2N2M0 IIIa NO 19+ wt NI NI T15 SCC 80 M MODERATE T3N0M0 IIb NO 19 wt NI NI T16 SCC 67 M NI T2N0M0 Ib NO 18+ wt NI NI T17 SCC 66 M MODERATE T2N0M0 Ib
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