Atanas Dimitrov Radkov

ATANAS DIMITROV RADKOV

Department of Biophysics and Biochemistry University of California – San Francisco N376 Genentech Hall600 16th Str San Francisco, CA 94158 ResearchGate: Atanas D Radkov [email protected]

EDUCATION: • University of Kentucky Lexington, Kentucky Doctor of Philosophy, Department of Plant and Soil Sciences, October 2015 Dissertation: “Unveiling novel aspects of D-amino acid metabolism in the model bacterium Pseudomonas putida KT2440” Program: Plant Physiology • University of Wisconsin – Oshkosh Oshkosh, Wisconsin Bachelor of Arts May 2010 Major: Cellular and Molecular Biology

RESEARCH POSITIONS: • Postdoctoral scholar University of California – San Francisco, October 2017 – present • Postdoctoral associate Indiana University, October 2015 – October 2017 • Graduate research assistant University of Kentucky, August 2010 – October 2015 • Undergraduate research assistant University of Wisconsin – Oshkosh o Wheat seed physiology, May 2009 – May 2010 o Organic chemistry synthesis, May 2008 – May 2010 o Chemistry stockroom, September 2007 – August 2009

TEACHING AND MENTORING: • Training: 2015 – 2016 American Society for Microbiology Science Teaching Fellows Program o Teaching certificate o Eight months online webinar course – six webinars each accompanied by a pre- and post-webinar assignment • Agricultural biotechnology 495 teaching assistant – Fall 2011; Fall 2012; Fall 2013 o College of Agriculture, University of Kentucky • Individual mentoring of undergraduate and graduate students, and visiting scholars – Spring/Fall 2013, Spring/Fall 2014, Fall 2015 to Fall 2017, Spring 2018 to present o Department of Plant and Soil Sciences, University of Kentucky o Department of Chemistry, Indiana University o Department of Biochemistry and Biophysics, University of California – San Francisco • Invited lectures o Department of Biology, Sonoma State University

Manuela Richter – Graduate student in TETRAD program (University of California – San Francisco) Ady Steinbach – Graduate student in TETRAD program (University of California – San Francisco) Steven Massa – Undergraduate student in Molecular Biology (University of California Berkeley) Garrett Booher – Doctoral student in Biochemistry (Indiana University) Jake Yablonowski – Master’s student in Biochemistry (Indiana University) Kory Brinker – Master’s degree student in Public Health (University of Kentucky) Katlyn McNeill – Undergraduate student; Major in Agricultural Biotechnology (University of Kentucky) Professor Qingxiang Yang – visiting scholar, faculty at Henan Normal University, China Professor Koji Uda – visiting scholar, Faculty of Science, Kochi University of Technology, Japan

REFEREED PUBLICATIONS: • Radkov, A D and Moe, L A. 2018. “A broad spectrum racemase in Pseudomonas putida KT2440 plays a key role in amino acid catabolism”. Frontiers in Microbiology. Accepted. • Hernández-Rocamora, V M; Otten, C F; Radkov, A D; Simorre, J-P; Breukink, E; VanNieuwenhze, M S; Vollmer, W. 2018. “Coupling of polymerase and carrier lipid phosphatase prevents product inhibition in peptidoglycan synthesis”. The Cell Surface. 2:1-13. • Radkov, A D; Hsu, Y-P; Booher, G; and VanNieuwenhze, M S. 2018. “Imagining bacterial cell wall biosynthesis”. Annual Review of Biochemistry. Epub ahead of print. • Uda, K; Abe, K; Dehara, Y; Mizobata, K; Edashige, Y; Nishimura, R; Radkov, A D; and Moe, L A. 2017. “Triple serine loop region regulates the aspartate racemase activity of the serine/aspartate racemase family”. Amino acids. Amino acids. 49(10):1743-1754. • Uda, K; Abe, K; Dehara, Y; Mizobata, K; Sogawa, N; Akagi, Y; Saigan, M; Radkov, A D; and Moe, L A. 2016. “Distribution and evolution of the serine/aspartate racemase family in invertebrates”. Amino Acids. 48(2):387-402. • Radkov, A D; McNeill, K*; Uda, K; and Moe, L A. 2016. " D-Amino acid catabolism is common among soil- and rhizosphere-dwelling bacteria". Microbes and Environments. 31(2):165-168. (*undergraduate student collaboration) • Radkov, A D and Moe, L A. 2014. “Bacterial synthesis of D-amino acids”. Applied Microbiology and Biotechnology. 98(12):5363-74. • Radkov, A D and Moe, L A. 2014. “Amino acid racemase enzyme assays”. Bio-protocol. 4(9):e1112. http://www.bio-protocol.org/e1112. • Radkov, A D and Moe, L A. 2013. “Amino acid racemization in Pseudomonas putida KT2440”. Journal of Bacteriology. 195(22):5016. • Seagren, J; Radkov, A D; David, S. 2009. “The total synthesis of (S)-2,4-dihydroxy-1-butyl (4-hydroxyl) benzoate”. Tetrahedron Letters. 50(27):3827.

TECHNICAL SKILLS: • Ten years of laboratory experience in molecular biology and biochemistry • Worked with a variety of enzymes, amino acid racemase, dehydrogenase and oxidoreductase, esterase, phosphatase, penicillin-binding proteins, L,D-transpeptidase, sortase, α-amylase, isoprenoid synthase, and multiple peptidoglycan amidase enzymes • Developed a new approach to study bacterial cell wall-enzyme interaction using Deep Mutational Scanning and Next-generation sequencing • Vector design, gene cloning, protein purification, DNA sequencing, PCR • High-throughput enzyme assay development • Developed first ever real-time penicillin-binding protein enzyme assay • Developed assays to elucidate natural product antibiotics mode of action • Genomic and metagenomic library construction • Fluorescence microscopy • Mutant construction in E. coli and Pseudomonas putida • Characterized a novel L-proline biosynthesis pathway in Pseudomonas putida • Developed a novel HPLC assay to study amino acid racemase enzymes

PROFESSIONAL DEVELOPMENT: • Scientific meetings: o 2018 Gordon Research Conference, Bacterial Cell Surfaces – West Dover, VT o 2016 Gordon Research Conference, Bacterial Cell Surfaces – West Dover, VT o 2014 American Society for Microbiology General Meeting – Boston, MA o 2013 Midwestern Enzyme Chemistry Conference – Loyola University, Chicago, IL o 2013 American Society for Microbiology, KY-TN regional meeting – Western Kentucky University, Bowling Green, KY o 2012 American Society for Microbiology, KY-TN regional meeting – Maryville College, Maryville, TN