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Profil Protein Seropositif Dan Seronegatif Bovine Viral Diarrhea Virus (Bvdv) Pada Sapi-Sapi Impor

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Profil Protein Seropositif Dan Seronegatif Bovine Viral Diarrhea Virus (Bvdv) Pada Sapi-Sapi Impor PROFIL PROTEIN SEROPOSITIF DAN SERONEGATIF BOVINE VIRAL DIARRHEA VIRUS (BVDV) PADA SAPI-SAPI IMPOR ANA ROUDLOTUL JANNAH PROGRAM STUDI BIOLOGI FAKULTAS SAINS DAN TEKNOLOGI UNIVERSITAS ISLAM NEGERI SYARIF HIDAYATULLAH JAKARTA 2019 M/ 1440 H PROFIL PROTEIN SEROPOSITIF DAN SERONEGATIF BOVINE VIRAL DIARRHEA VIRUS (BVDV) PADA SAPI-SAPI IMPOR SKRIPSI Sebagai Salah Satu Syarat untuk Memperoleh Gelar Sarjana Sains Pada Program Studi Biologi Fakultas Sains dan Teknologi Universitas Islam Negeri Syarif Hidayatullah Jakarta ANA ROUDLOTUL JANNAH 1113095000030 PROGRAM STUDI BIOLOGI FAKULTAS SAINS DAN TEKNOLOGI UNIVERSITAS ISLAM NEGERI SYARIF HIDAYATULLAH JAKARTA 2019 M/ 1440 H ABSTRAK ANA ROUDLOTUL JANNAH. Profil Protein Seropositif dan Seronegatif Bovine Viral Diarrhea Virus (BVDV) Pada Sapi-Sapi Impor. Skripsi. Program Studi Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Syarif Hidayatullah Jakarta. Dibimbing oleh Dr. Muharam Saepulloh, S.Si., M.Sc. dan drh. RR. Bhintarti Suryohastari, M. Biomed. 2018. Seiring impor sapi yang dilakukan pemerintah untuk memenuhi kebutuhan pangan masyarakat, mengakibatkan salah satu penyakit menular yaitu Bovine Viral Diarrhea Virus (BVDV) dapat masuk ke Indonesia. Bovine Viral Diarrhea (BVD) diprioritaskan pemberantasannya karena dapat menyebabkan kematian pada sapi dan menimbulkan kerugian ekonomi. Penelitian ini bertujuan untuk mengetahui profil protein berdasarkan berat molekul (BM) pada hasil ELISA antibodi terhadap BVDV. Sapi yang diperiksa menggunkan ELISA adalah sapi Peranakan Ongole, Limosin, Simental, Angus, Brangus, Brahman, Wagyu, dan Frisien Holstein di Balai Embrio Ternak (BET), Bogor, Indonesia. Penelitian ini dilakukan dengan analisis SDS-PAGE terhadap seropositif dan seronegatif BVDV pada serum sapi. Sebanyak 43 serum sapi yang diujikan, 21 sampel seropositif BVDV (49%) dan 22 sampel seronegatif BVDV (51%). Hasil SDS-PAGE menunjukkan pita protein dengan BM 151, 397 kDa yang diduga IgG seropositif BVDV pada sapi Friesian Holstein dan pita dengan BM 55, 350 kDa yang diduga antigen seropositif BVDV pada sapi Frisien Holstein dan Wagyu. Sementara itu, pita protein IgG dan antigen virus BVDV tidak terdapat pada seluruh sapi yang memiliki seronegatif antibodi BVDV. Pada seropositif BVDV ditemukan pita protein Ig dan pita protein yang diduga sebagai antigen BVDV, sedangkan pada seronegatif BVDV tidak terdapat pita protein Ig dan antigen BVDV. Hasil penelitian diharapkan dapat sebagai data yang membantu tindakan pencegahan dan pemberantasan BVDV di Indonesia. Kata kunci: BVDV, Immunoglobulin, Profil protein, SDS-PAGE, ELISA i ABSTRACT ANA ROUDLOTUL JANNAH. Porifl Protein of Bovine Viral Diarrhea Virus (BVDV) Seropositive and Seronegative on an Imported Cattles. Essay. Biology Studies Program, Faculty of Science and Technology, Syarif Hidayatullah State Islamic University Jakarta. Under Guidance of Dr. Muharam Saepulloh, S.Si., M.Sc. and drh. RR. Bhintarti Suryohastari, M. Biomed. 2018. Along with the import of cattle by the government to meet the food needs of the people, one of the infectious diseases, BVDV (Bovine Viral Diarrhea Virus), can enter Indonesia. Bovine Viral Diarrhea got eradication prioritizes because it can cause death in cattle and cause economic losses. This study aims to determine the protein profile based on molecular weight (BM) on the ELISA antibody results on BVDV. The Cattles that examined using ELISA were Ongole, Limosin, Simental, Angus, Brangus, Brahman, Wagyu, Frisien Holstein at the Livestock Embryo Center (LEC), Bogor, Indonesia. This study was carried out by detecting the profile of the ELISA seropositive and seronegative immunoglobulin protein results using SDS-PAGE. Serum is taken from Holstein Friesian, Ongole, Limousine, Simental, Angus, Brangus, Brahman, and Wagyu cattle breeds. The ELISA results showed that from 43 bovine serums tested, 21 samples were BVDV seropositive (49%) and 22 samples were seronegative BVDV (51%). The SDS- PAGE results showed that protein bands with BM 151, 397 kDa suspected as BVDV seropositive IgG from Holstein Friesian cattle and protein bands with BM 55, 350 kDa suspected as BVDV seropositive antigens from Frisien Holstein and Wagyu cattle. Mean while, All antibody seronegative BVDV cattle did not show IgG protein bands and BVDV antigens. The results of the study are expected to be used as data for the prevention and eradication of BVDV in Indonesia. Keywords: BVDV, Immunoglobulin, Protein profile, SDS-PAGE, ELISA ii KATA PENGANTAR Alhamdulillah, puji syukur kepada Allah Subhanahu wa Ta’ala Rabb semesta alam, atas nikmat ilmu pengetahuan yang diberikan sehingga penulis dapat menyelesaikan skripi. Penelitian ini merupakan salah satu syarat dalam memperoleh gelar Sarjana Sains pada Program Studi Biologi Fakultas Sains dan Teknologi Universitas Islam Negeri Syarif Hidayatullah Jakarta. Skripsi ini berjudul Profil Protein Seropositif dan Seronegatif Bovine Viral Diarrhea Virus (BVDV) Pada Sapi-Sapi Impor. Penelitian ini dilaksanakan di Balai Besar Penelitian Veteriner, Bogor. Penulis mengucapkan terima kasih kepada semua pihak yang telah banyak membantu dan memberikan dukungan baik dalam bentuk moril maupun materil, yaitu kepada: 1. Kedua orang tua, kaka dan adik, terima kasih atas doa dan dukungan yang tak henti-hentinya. 2. Dr. Agus Salim, M.Si, selaku Dekan Fakultas Sains dan Teknologi UIN Syarif Hidayatullah Jakarta. 3. Dr. Dasumiati M.Si., selaku Ketua Program Studi Biologi Fakultas Sains dan Teknologi UIN Syarif Hidayatullah Jakarta, serta selaku penguji I pada sidang munaqosah penelitian yang telah memberikan masukannya kepada penulis. 4. Dr. Muharam Saepulloh, S.Si., M.Sc. selaku pembimbing I atas kesediaan dalam membimbing dan memberikan ilmu, arahan, saran, dan nasihat yang bermanfaat kepada penulis. 5. drh. R.R. Bhintarti Suryohastari M. Biomed., selaku pembimbing II atas kesediaan dalam membimbing dan memberikan pengarahan, sehingga penulis dapat menyelesaikan skripsi ini iii 6. Dr. Megga Ratnasari Pikoli dan Dr. Nani Radiastuti selaku dosen penguji I dan penguji II pada seminar proposal dan seminar hasil penelitian yang telah memberikan masukan kepada penulis 7. Etyn Yunita, M.Si, selaku penguji I dan penguji II pada sidang munaqosah penelitian yang telah memberikan masukannya kepada penulis. 8. Seluruh dosen yang telah mendidik penulis selama menuntut ilmu di Program Studi Biologi, FST UIN Syarif Hidayatullah Jakarta. 9. Pak Puji, selaku pembimbing di laboratorium BBLITVET yang selalu sabar mendampingi saya dan atas ilmu yang diberikan, serta para bapak yang membantu dalam pengambilan sampel serum. 10. Festy Auliyaur Rahmah, S. Si, serta laboran lainnya yang membantu penelitian di laboratorium UIN Jakarta. 11. Rekan mahasiswa Jurusan Biologi 2013 yang bersama-sama dengan penulis menghadapi suka duka selama menempuh pendidikan. Teman SMA yang selalu memberikan membantu dan memberikan motivasi kepada penulis 12. Semua pihak yang telah membantu penulis dalam menyelesaikan penelitian. Penulis menyadari penyusunan skripsi ini masih jauh dari sempurna. Penulis berharap semoga skripsi ini dapat bermanfaat bagi pembaca. Jakarta, 24 Januari 2019 Penulis iv DAFTAR ISI Halaman PERNYATAAN ............................................................................................................. 3 ABSTRAK ..................................................................................................................... i ABSTRACT .................................................................................................................. ii KATA PENGANTAR ................................................................................................... iii DAFTAR ISI ................................................................................................................. v DAFTAR GAMBAR .................................................................................................. vii DAFTAR TABEL ....................................................................................................... viii DAFTAR LAMPIRAN ................................................................................................ ix BAB I PENDAHULUAN ............................................................................................. 1 1.1 Latar Belakang .................................................................................................... 1 1.2 Rumusan Masalah ............................................................................................... 1 1.3 Hipotesis .............................................................................................................. 3 1.4 Tujuan Penelitian ................................................................................................. 3 1.5 Manfaat Penelitian .............................................................................................. 3 1.6 Kerangka Berpikir ............................................................................................... 4 BAB II TINJAUAN PUSTAKA ................................................................................... 5 2.1 Protein ................................................................................................................. 5 2.1.1 Penggolongan protein................................................................................... 6 2.2 Serum .................................................................................................................. 7 2.3 Imunoglobulin ....................................................................................................
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