Evaluation of Bacillus Spp. As Dough Starters for Adhirasam-A Traditional
Brazilian Journal of Microbiology 46, 4, 1183-1191 (2015) Copyright © 2015, Sociedade Brasileira de Microbiologia ISSN 1678-4405 www.sbmicrobiologia.org.br DOI: http://dx.doi.org/10.1590/S1517-838246420140409 Research Paper Evaluation of Bacillus spp. as dough starters for Adhirasam - A traditional rice based fermented food of Southern India Anvar Hussain Noorul Anisha1, Rangasamy Anandham2, Soon Woo Kwon3, Pandiyan Indira Gandhi4, Nellaiappan Olaganathan Gopal2 1Department of Food Science, Tamil Nadu Agricultural University, Tamil Nadu, India. 2Department of Agricultural Microbiology, Tamil Nadu Agricultural University, Tamil Nadu, India. 3Korean Agricultural Culture Collection, National Academy of Agricultural Science, Republic of Korea. 4Regional Research Station, Tamil Nadu Agricultural University, Tamil Nadu, India. Submitted: May 14, 2014; Approved: February 16, 2015. Abstract Adhirasam is a cereal based, doughnut shaped, deep fried dessert consumed in the southern regions of India. The dough used to prepare adhirasam is fermented and contains rice flour and jaggery. The aim of the present study was to characterize the cultivable bacteria associated with this fermented dough and to identify a suitable starter culture for the production of quality adhirasam. In total, one hundred and seventy bacterial isolates were recovered from de Man Rogosa Sharp (MRS) agar, nutri- ent agar, lysogeny agar and tryptic soy agar media. Out of the 170 bacterial isolates, sixteen isolates were selected based on their ability to tolerate glucose and sucrose. All the bacterial isolates tolerated 15% glucose and 30% sucrose. Analyses of 16S rDNA gene sequences of the bacterial isolates showed that the dominant cultivable bacteria were members of the genus Bacillus. These strains were further used as starters and tested for their ability to ferment rice flour with jaggery to produce adhirasam dough.
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