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Dikarya F Talbotiomyces versus Entorrhizomycota Entorrhiza Key words: Entorrhiza % Talbotiomyces Talbotiomyces calosporus Talbotiomyces et al.566#] % % et al. 56!4 O IMA FUNGUS · 6(1): 129–133 (2015) 129–133 · 6(1): FUNGUS IMA

Entorrhizomycota < + 4 X Talbotiomyces Poales T. calosporus T. !"7_ [% % Entorrhiza Entorrhiza Entorrhiza Entorrhizomycota Entorrhiza $ 2X et al.56!4 7 Caryophyllales Talbotiomyces calosporus Talbotiomyces Glomeromycota O V <>/ 56!556!_<%I<%56!5< + O % \ % % % @ % < W !"E6<IQ !"E! . + Entorrhiza calospora @ / Talbotiomycetaceae Entorrhiza Entorrhizomycota Juncaceae U ; = 3 Talbotiomyces % Dikarya, Basidiomycota, % Entorrhizaceae, Entorrhizomycota Cyperaceae Cyperaceae Entorrhiza U 2 5 Entorrhizomycetes, Entorrhiza et al.!""#+ Talbotiomycetales T. calosporus T. Talbotiomyces Talbotiomyces et al. 56!4 W !"#E X !""7 Ascomycota Talbotiomyces 2 ! Entorrhiza

Juncaceae Entorrhizomycetidae Tilletiaceae !"4_ N +

, !7 ! Entorrhizomycota V . Entorrhizomycota 9%

[ Entorrhiza Entorrhizomycota Entorrhizomycota ] .+ .@ %4#56#FV ] Z % +/]] Z % %!4677#E6+] V@ * > ; > > 7#Z9Z2 W / + N Z % ; G * Z % 9 [66E_; @ / @O N + ; / @ < U* O7F;*._!.4!52 ; Z % V N S% S; S% S/ U !#56#FV ] K V $ 9 $ within the Article info:@ [FU 56!4K/ [!FU 56!4K; [5FU 56!4 <>/? + $ % [ incertae sedis Entorrhiza Abstract: . [ ^ . © 2015 International Mycological Association © 2015 International Mycological conditions: distribute and transmit the work, under the following are free to share - to copy, You Attribution: F 5 3 7 4 ! 2 #"$"%"' on eudicotyledons ord. nov. : Talbotiomycetales Entorrhizomycota Non-commercial:No derivative works:Any of the above conditions can be waived if you get For any reuse or distribution, you must make clear to others the license terms of this work, which can be found at http://creativecommons.org/licenses/by-nc-nd/3.0/legalcode. Nothing in this license impairs or restricts the author’s moral rights. permission from the copyright holder. that ? % Entorrhizales, within the Ustilaginomycotina+ et al. !""E 566# + Entorrhiza INTRODUCTION Juncaceae V % Fungi V [ [ [!644"E\ 56!46F6!6# VOLUME 6 · NO. 1 + et al.56!4 Entorrhiza Cyperaceae Riess et al.

MATERIALS AND METHODS [ ] % 6"! G 5666 % Material $ [Q Specimen examined[ Talbotiomyces calosporus[ South RV[ @@Z Africa: ] [ ; + U W !7"E *@Z 4!7 W

ARTICLE Limeum viscosum L. glomeratum _6 ` !"7_ J.J.O. Pazzi+ N;[ 9ZX 4E#M ; SU __##6 U $ M U* !666 ]V G/V DNA isolation, PCR, and sequencing /$U* % E6!# @ 56!7 / V <>/ $ N; S; ; <>/ 2 / ` ` + UGUG U + % R V @@Z <>/ _55 ? et al.56!5 ]V ]S [ % 4&!6F >@! >@E et al. !""6 >@!# ; % >@57] IV !""5;G !66 [ 54&!6F ; 9 .W <>/ ; W O Q . V % X W . Talbotiomyces Entorrhiza were $ . ? 4 G % _6 U? %5#4U IU 56!! V4a *@Z <>/ [ * 6 X * [\\ \ \ \ RESULTS * F X I 9 !""6 ;G $ 4 )* ;G !6$ Phylogenetic placement of Talbotiomyces

6#4UUG546U!74)* !)*>V; V $4U646)* 54\)*654)* <>/?Talbotiomyces calosporus[% U Taq <>/ + * 5 Entorrhiza, % Z\)* 54 )* <>/ ;G Glomeromycota W ! N [[!6_6 % @@Z *@Z "7 G74 F6 G.! G #4 #5 W ! Entorrhiza G 5F_6 "7 G74 46 G Talbotiomyces #4 #5 G [ $ # #5 G;G V T. calosporus [ S$@/;.NVZ@+G ## 46z ` G% Q9 ![56 G !E@ 4# "6z E. aschersoniana*@ZN ? @@Z *@Z <>/ % ![F + <V %_!G@? 2 Entorrhiza\Talbotiomyces / + W G G/ [ Dikarya ""zU* !66+ @? /+N ; W ! _!_6xl ] / / + W % ? @? % 7! ] TAXONOMY G G // UN V <>/ ? Talbotiomyces calosporus % Q Talbotiomyces calosporus ]+ EntorrhizomycotaU % 2`FFF!5"@@Z 2;7!_64F*@Z Entorrhiza Talbotiomyces Molecular phylogenetic analyses [ [ $ % V Talbotiomyces, % Talbotiomyces, <>/ @@Z *@Z Talbotiomyces ? + ? Entorrhizomycetes[ T. calosporus Entorrhiza? % ]+ % Talbotiomycetales 2 + 2 Glomeromycota W ! 2 ; = XI+ ord. nov. V Gromochytrium mamkaevae Olpidium U+ U+E!6#"# brassicae $ W ]+ V +/@S Diagnosis: U Entorrhizomycetes+ et al N< !#F67 / 566# % U/WWV % #!7# 2 I @ 56!_ S.N>@. 2 et al. 5664. N Type[TalbotiomycesXet al.566#

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Fig. 1. ; Talbotiomyces calosporus+ U* % @@Z *@Z <>/ ?Talbotiomyces, Entorrhiza %Ascomycota, Basidiomycota Glomeromycota+ % U* 1#6\+ 16"6V Gromochytrium mamkaevae Olpidium brassicae

Talbotiomycetaceae 2 + 2 V Talbotiomyces 2 ; = XI+ fam. nov. Entorrhiza U+ U+E!6#"E + % Diagnosis: U Talbotiomycetales % Entorrhiza Talbotiomyces % Type[TalbotiomycesXet al.566# . V !"4F W !"E6 < IQ !"E!+ et al.!""#566!56!4X et al.566#9% DISCUSSION Entorrhiza TalbotiomycesEntorrhiza is O Entorrhiza/Talbotiomyces relationship Cyperaceae Juncaceae+ et al.!""#566!N Talbotiomyces calosporus % T. calosporus Entorrhiza @@Z *@Z <>/ W ! CaryophyllalesXet al.566#V

VOLUME 6 · NO. 1 131 Riess et al.

% Entorrhizomycota Talbotiomyces calosporus 9ZX 2 [ Entorrhizales Talbotiomycetales X + N; @ ] V V Z % / UV W/+N ]@ % Talbotiomyces Entorrhiza % V % Entorrhizomycota V ] W

ARTICLE Talbotiomyces Entorrhizomycota, % % + et al.56!4. REFERENCES

Coevolution and presumed host spectrum + + < Q W ; U + U* @ Entorrhiza Talbotiomyces 566! Ustilaginomycetes N[ The Mycota. X # ; + % Systematics and Evolution. U* <` U* S] Entorrhiza *;/[4#ME_+ [@ Cyperaceae Juncaceae + ] @Q W 2 %Uet al.56!4 X Entorrhizomycota[ % % 56!5 Talbotiomyces calosporus % FungiPLoS ONE[ @ / Limeum viscosum + * OU+ <; = UX2et al.566E/ Molluginaceae Trianthema pentandraAizoaceae Mycological Research 112[!5"#M!_6F V !"4F W X et al. 566# + Q WX2!""#Z Portulaca oleraceaPortulacaceae $ Canadian Journal of % 9ZX 5!_"# Botany 75[!5#_M!_!7 V 2X[ON [ + * @ + 56!5 Annonaceae Entorrhiza calospora P V T. calosporus\ NN[ Dasyspora + I U/ G Mycologia 104[ P. oleracea\ [ N F4"MFE! T. calosporus + < + Q W !""E O!""#P~ ; *@Z <>/ ? . $ Canadian Journal of Botany 75[5674M564F + < @ U + 566# O566FP~ / + et al.566E* Oet al. Ustilaginomycotina 566E] et al.566";et al.56!!+et al.56!5 Mycologia 98["6FM"!F * 9 56!_; = et al.56!556!_X O G ` 5666 @ % et al.56!7X et al.56!7UV et al.56!4< Molecular Entorrhizomycota Biology and Evolution 17[476M445 + et al. 56!4 O <]Q W!"E!@ ; % \ 7N% Entorrhiza casparyana % V Talbotiomyces in Mycologia 73[_"5M_"E Entorrhizomycota$ <% GU <% VV 56!5 > F Cyperaceae\JuncaceaePoales / Mycotaxon 121[5!4M55_ Entorrhizomycota W `U!"#E/ $ % EntorrhizaG F66U K+ et al.56!4 UstilaginalesNova Hedwigia 30[!MFE !46 U K et al. 566! V W `U !"E6 V Entorrhiza Entorrhizomycota % G Ustilaginales Cyperaceae . % < JuncaceaeNova Hedwigia 32[5F4M5E7 %. ] / V ` !""5 ; ;G Entorrhizomycota [ ? !E@ <>/ % % O Mycologia 84[4E"M4"5 % / Entorrhiza\ ] @ 2 + Q W % U 56!_ Talbotiomyces ; % V$ % [ Entorrhizomycota FEMS Microbiology Ecology 83[5F4M5#E . O ] UX 9+O?O]]Q W566"@ Sebacinales %et [ Hyaloperonospora al.56!!] et al.56!_ NV@ *@Z ? Mycological Research 113[_6EM_54 2 2 2 2 V 9 U V 5664 U/WWV % 4[ ACKNOWLEDGEMENTS % ? Nucleic Acids Research 33[4!!M4!E + !"46M56!7 $ 2 2@

132 IMA FUNGUS Talbotiomycetales ord. nov. (Entorrhizomycota)

* U9 @56!_* $ X 2 56!5 Smut Fungi of the World @ ; U>[/ ARTICLE % Puccinia coronata $ ; @ ; Mycological Progress 12[F_ME" X256!_Illustrated Genera of Smut Fungi_ @ ; * O U ; = U 2 U G / Q W 566E U>[/ ; @ ; / Caryophyllaceae[ % X2+ + <566#Talbotiomyces Mycological Research 112[!5E6M!5"F Entorrhiza calosporaBasidiomycotaMycologia Balcanica U ;U < 56!!U? [ 4[!!M!7 % X 5#4 [\\? X O / <@ W9 S2 2 Uet al.56!7 UV / < G ] /< / UG @ % < % Thecaphora schwarzmaniana Rheum ribes in ] 56!4 / .% $ N V [ % EndoraeciumPucciniales Acacia / Mycological Progress 13[EE!ME"5 Persoonia 35[46MF5 X 9 U !""6 [ ; = U * O U G /Q 56!_ G % O [ <>/ % Antherospora vaillantii$Muscari speciesIMA Fungus Cryptococcus speciesJournal of Bacteriology 172[75_EM757F 4[4M!" X 9 U .+ U * ++ 56!7 < ; = U* OU /2 UU .+ Z56!5 Peronospora Papaver[ $ Microbotryum heliospermae Heliosperma pusillum S Papaver somniferum PLoS ONE 9[ / @ Fungal Biology 116[!E4M!"4 "FE_E ; @ V@ G ` G `9 ; U et al. 56!! % U @" % ] @ 2 U W et al. 56!! V Pustula Sebacinales % [ % % ? % % % PLoS ONE 6[!F#"_ Fungal V` + V< * @ V ` !""6 / [ Biology 115[5!7M5!" ? >/ N[ ? WVUX U ;/ <*< /et al. PCR Protocols: a guide to methods and applicationsN U/ 56!5 U + _5[ [ + ] <9@ `` V`[_!4M_55@ < [ Systematic / ; Biology 61[4_"M475 > @ % X G U 566! S% @ / 56!7 /$U* % E[ [ G Proceedings of the . Bioinformatics Royal Society London B, Biological Sciences 268[55!!M5556 30[!_!5M!_!_ ]* !"4_ The Ustilaginales of the World G V ;9+ !"4F Entorrhiza calospora % !#F~Z % ; ;>[;% @ G@ Limeum Bothalia 6[74_M7F_ / X2!""7European Smut Fungi` [] %W

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