IMA FUNGUS · 6(1): 129–133 (2015) [!644"E\ 56!46F6!6# #"$"%"' ARTICLE Entorrhizomycota: Talbotiomycetales ord. nov. on eudicotyledons 2 !, + !2 5U 2 3U ; = 72X4 < + F !Z % V N S% S; S% S/ U !#56#FV ] K . [ ^ . 5V@ * > ; > >7#Z9Z2 3W / + N Z % ; G * Z % 9 [66E_; @ / 7@O N + ; / @ < U* O7F;*._!.4!52 ; 4] .+ .@ %4#56#FV ] FZ % +/]] Z % %!4677#E6+] Abstract: V Entorrhizomycota Key words: Entorrhiza . Cyperaceae JuncaceaeTalbotiomyces calosporus Entorrhiza incertae sedis Entorrhiza % % [ T. calosporus Entorrhizomycota <>/?Talbotiomyces %Entorrhiza $ + Talbotiomyces $ Entorrhiza Talbotiomyces within the Entorrhizomycota Entorrhiza Talbotiomyces V $ Entorrhizomycota Poales 9 $ Talbotiomycetales % Talbotiomycetaceae % Talbotiomyces Article info:@ [FU 56!4K/ [!FU 56!4K; [5FU 56!4 INTRODUCTION 56!556!_<%I<%56!5< Entorrhizomycota versus Entorrhiza + et al. 56!4 O Cyperaceae O % Juncaceae V \ % % % % @ Entorrhiza Fungi V % [ Tilletiaceae !"4_ N < [ . Entorrhiza W !"E6<IQ !"E! Entorrhizaceae, + et al.56!4Talbotiomyces Entorrhizales, Entorrhizomycetidae Entorrhizomycetes, Entorrhiza within the Ustilaginomycotina+ et al.!""#+ Entorrhiza calospora et al. !""E 566# + et al. 56!4 @ / that Entorrhiza CaryophyllalesXet al.566#] % ? % Talbotiomyces calosporus Entorrhizomycota 9% Entorrhiza Talbotiomyces Entorrhizomycota Dikarya, O Entorrhizomycota Ascomycota Basidiomycota, V <>/ + et al.56!4 T. calosporus !"7_ [% Entorrhizomycota Entorrhiza % Dikarya Entorrhiza !7 Glomeromycota Cyperaceae Juncaceae W !"#E X !""7 © 2015 International Mycological Association You are free to share - to copy, distribute and transmit the work, under the following conditions: Attribution: [ Non-commercial: No derivative works: For any reuse or distribution, you must make clear to others the license terms of this work, which can be found at http://creativecommons.org/licenses/by-nc-nd/3.0/legalcode. Any of the above conditions can be waived if you get permission from the copyright holder. Nothing in this license impairs or restricts the author’s moral rights. VOLUME 6 · NO. 1 129 Riess et al. MATERIALS AND METHODS [ ] % 6"! G 5666 % Material $ [Q Specimen examined[ Talbotiomyces calosporus[ South RV[ @@Z Africa: ] [ ; + U W !7"E *@Z 4!7 W ARTICLE Limeum viscosum L. glomeratum _6 ` !"7_ J.J.O. Pazzi+N;[ 9ZX 4E#M ;SU __##6 U $ M U* !666 ]VG/V DNA isolation, PCR, and sequencing /$U* % E6!# @ 56!7 / V <>/ $ N;S; ; <>/ 2 / ` ` + UGUG U + % R V @@Z <>/ _55? et al.56!5 ]V]S [ % 4&!6F >@! >@E et al. !""6 >@!# ; % >@57] IV !""5;G !66 [ 54&!6F ; 9 .W <>/ ; W O Q . V % X W . Talbotiomyces Entorrhiza were $ . ? 4G % _6 U? %5#4U IU 56!! V4a *@Z <>/ [ *6 X * [\\ \ \ \ RESULTS *F X I 9 !""6 ;G $ 4 )* ;G !6$ Phylogenetic placement of Talbotiomyces 6#4UUG546U!74)* !)*>V; V $4U646)* 54\)*654)* <>/?Talbotiomyces calosporus[% U Taq <>/ + * 5 Entorrhiza, % Z\)* 54 )* <>/ ;G Glomeromycota W ! N [[!6_6 % @@Z *@Z "7G74 F6G.!G #4 #5 W ! Entorrhiza G 5F_6 "7G74 46G Talbotiomyces #4 #5G [ $ # #5G;G V T. calosporus [ S$@/;.NVZ@+G ## 46z `[ G% Q9 ![56 G !E@ 4# "6z E. aschersoniana*@ZN ? @@Z *@Z <>/ % ![F + <V %_!G@? 2 Entorrhiza\Talbotiomyces / + W G G/ [ Dikarya ""zU* !66+ @? /+N ; W ! _!_6xl ] / / + W % ? @? % 7! ] TAXONOMY G G // UN V <>/ ? Talbotiomyces calosporus % Q Talbotiomyces calosporus ]+ EntorrhizomycotaU % 2`FFF!5"@@Z 2;7!_64F*@Z Entorrhiza Talbotiomyces Molecular phylogenetic analyses [ [ $ % V Talbotiomyces, % Talbotiomyces, <>/ @@Z *@Z Talbotiomyces ? + ? Entorrhizomycetes[ T. calosporus Entorrhiza? % ]+ % Talbotiomycetales 2 + 2 Glomeromycota W ! 2 ; = XI+ ord. nov. V Gromochytrium mamkaevae Olpidium U+ U+E!6#"# brassicae $ W ]+ V +/@S Diagnosis: U Entorrhizomycetes+ et al N< !#F67 / 566# % U/WWV % #!7# 2 I @ 56!_ S.N>@. 2 et al. 5664. N Type[TalbotiomycesXet al.566# 130 IMA FUNGUS Talbotiomycetales ord. nov. (Entorrhizomycota) ARTICLE ½½¾ *$" ½¾ ½½¾ !" ½½¾ ¿ ¿ ~ ½½¾ $) ½½¾ ½½¾ !" ¡~ ½½¾ ½¾ +0''12 À¾À ~ ½¾ ½½¾ ½½¾ !" ½¾ !"#$%"$&'($ ½½¾ 3#'!" ~ }~ ¾À ½½¾ Fig. 1. ; Talbotiomyces calosporus+ U* % @@Z *@Z <>/ ?Talbotiomyces, Entorrhiza %Ascomycota, Basidiomycota Glomeromycota+ % U* 1#6\+ 16"6V Gromochytrium mamkaevae Olpidium brassicae Talbotiomycetaceae 2 + 2 V Talbotiomyces 2 ; = XI+ fam. nov. Entorrhiza U+ U+E!6#"E + % Diagnosis: U Talbotiomycetales % Entorrhiza Talbotiomyces % Type[TalbotiomycesXet al.566# . V !"4F W !"E6 < IQ !"E!+ et al.!""#566!56!4X et al.566#9% DISCUSSION Entorrhiza TalbotiomycesEntorrhiza is O Entorrhiza/Talbotiomyces relationship Cyperaceae Juncaceae+ et al.!""#566!N Talbotiomyces calosporus % T. calosporus Entorrhiza @@Z *@Z <>/ W ! CaryophyllalesXet al.566#V VOLUME 6 · NO. 1 131 Riess et al. % Entorrhizomycota Talbotiomyces calosporus 9ZX 2 [ Entorrhizales Talbotiomycetales X +N; @ ] V V Z % / UV W/+N ]@ % Talbotiomyces Entorrhiza % V % Entorrhizomycota V ] W <W]+/#4\_.!K+S556!\#.! ARTICLE Talbotiomyces Entorrhizomycota, % % + et al.56!4. REFERENCES Coevolution and presumed host spectrum + + < Q W ; U + U* @ Entorrhiza Talbotiomyces 566! Ustilaginomycetes N[ The Mycota. X # ; + % Systematics and Evolution. U* <` U* S] Entorrhiza *;/[4#ME_+ [@ Cyperaceae Juncaceae + ] @Q W 2 %Uet al.56!4 X Entorrhizomycota[ % % 56!5 Talbotiomyces calosporus % FungiPLoS ONE[ @ / Limeum viscosum + * OU+ <; = UX2et al.566E/ Molluginaceae Trianthema pentandraAizoaceae Mycological Research 112[!5"#M!_6F V !"4F W X et al. 566# + Q WX2!""#Z Portulaca oleraceaPortulacaceae $ Canadian Journal of % 9ZX 5!_"# Botany 75[!5#_M!_!7 V 2X[ON [ + * @ + 56!5 Annonaceae Entorrhiza calospora P V T. calosporus\ NN[ Dasyspora + I U/ G Mycologia 104[ P. oleracea\ [ N F4"MFE! T. calosporus + < + Q W !""E O!""#P~ ; *@Z <>/ ? . $ Canadian Journal of Botany 75[5674M564F + < @ U + 566# O566FP~ / + et al.566E* Oet al. Ustilaginomycotina 566E] et al.566";et al.56!!+et al.56!5 Mycologia 98["6FM"!F * 9 56!_; = et al.56!556!_X O G ` 5666 @ % et al.56!7X et al.56!7UV et al.56!4< Molecular Entorrhizomycota Biology and Evolution 17[476M445 + et al. 56!4 O <]Q W!"E!@ ; % \ 7N% Entorrhiza casparyana % V Talbotiomyces in Mycologia 73[_"5M_"E Entorrhizomycota$ <% GU <% VV 56!5 > F Cyperaceae\JuncaceaePoales / Mycotaxon 121[5!4M55_ Entorrhizomycota W `U!"#E/ $ % EntorrhizaG F66U K+ et al.56!4 UstilaginalesNova Hedwigia 30[!MFE !46 U K et al. 566! V W `U !"E6 V Entorrhiza Entorrhizomycota % G Ustilaginales Cyperaceae . % < JuncaceaeNova Hedwigia 32[5F4M5E7 %. ] / V ` !""5 ; ;G Entorrhizomycota [ ? !E@ <>/ % % O Mycologia 84[4E"M4"5 % / Entorrhiza\ ] @ 2 + Q W % U 56!_ Talbotiomyces ; % V$ % [ Entorrhizomycota FEMS Microbiology Ecology 83[5F4M5#E . O ] UX 9+O?O]]Q W566"@ Sebacinales %et [ Hyaloperonospora al.56!!] et al.56!_ NV@ *@Z ? Mycological Research 113[_6EM_54 2 2 2 2 V 9 U V 5664 U/WWV % 4[ ACKNOWLEDGEMENTS %.