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1_0131_Ali.qxp 1/2/07 12:36 Page 1 Antiviral Therapy 12:1–6 Inhibition of Simian virus 40 large T antigen helicase activity by fluoroquinolones Syed Hamid Ali1*, Anil Chandraker 2 and James A DeCaprio 3 1Department of Biological and Biomedical Sciences, The Aga Khan University, Karachi, Pakistan 2Transplantation Research Center, Brigham and Womens Hospital, Boston, MA, USA 3Department of Medical Oncology, Dana-Farber Cancer Institute, Boston, MA, USA *Corresponding author: Tel: +92 21 486 4435; Fax: +92 21 493 4294/493 2095; E-mail: [email protected] Background: Fluoroquinolones represent a potent group trovafloxacin, ciprofloxacin and ofloxacin, were tested of antibiotics that inhibit bacterial DNA replication by for their ability to inhibit viral DNA replication. targeting the essential bacterial enzymes gyrase and Results: We show here that all four quinolones tested topoisomerase IV. Inhibition of gyrase activity by were effective in the inhibition of SV40 plaque formation quinolones involves the interaction of these drugs with and DNA replication in CV1-P cells. In addition, we found the helicase component of bacterial gyrase. DNA that each of these quinolones was inhibitory to the heli- tumour viruses also encode helicases that are essential case activity of SV40 large tumour antigen. for their DNA replication in the host. Conclusions: Fluoroquinolones and their derivates may Methods: In this study we have evaluated the effect of therefore be useful in the treatment and/or prevention of fluoroquinolones on viral DNA replication using the DNA infection by SV40-homologous human DNA viruses that tumour virus Simian virus 40 (SV40) as our model. Four encode helicase activity for their survival. different fluoroquinolones, namely, levofloxacin, Introduction Simian virus 40 (SV40) large tumour antigen (T Ag) targets of (fluoro)quinolones [9]. In contrast with plays a key role in SV40-mediated transformation as another group of drugs, coumarins, which inhibit well as in viral replication. T Ag possesses several gyrase by targeting its ATPase subunit GyrB [10], biochemical activities including helicase, ATPase and quinolones block DNA replication by interacting with DNA-binding activities that are essential for viral the DNA-binding subunit of gyrase, GyrA, thus replication. In addition, Large T Ag serves to recruit converting gyrase into a cellular poison [11]. This cellular proteins including replication protein A, DNA quinolone poisoning of gyrase is effected by freezing of polymerase and topoisomerase I that participate in the gyrase–DNA complex into a ternary viral DNA replication [1]. Human DNA polyoma gyrase–quinolone–DNA complex, which leads to the viruses BKV and JCV encode SV40-homologous disruption of replication and double-stranded DNA T Ags that play a similar role in viral replication [2,3]. breaks, eventually resulting in cytotoxicity [12,13]. BK and JC viruses are thought to contribute to the To test whether SV40 could be inhibited by fluoro- pathogenesis of several human diseases including quinolones, the ability of levofloxacin, trovafloxacin, haemorrhagic cystitis, BK nephropathy and progres- ciprofloxacin and ofloxacin to inhibit viral DNA repli- sive multifocal leukoencephalopathy. cation was tested. We show here that all four In clinical trials, fluoroquinolones have been found quinolones tested were effective in the inhibition of to be effective against DNA [4] as well as RNA [5,6] SV40 plaque formation and DNA replication in CV1-P viruses. The ability of fluoroquinolones to alter cells. In addition, we found that each of these protein–DNA binding in vitro has shown promise for quinolones was inhibitory to the helicase activity of the therapy against HIV as well [7]. It has been previously SV40 T Ag. In view of what we report here, fluoro- reported that the fluoroquinolones nalidixic acid and quinolone drugs may be effective in treating infections oxolinic acid can inhibit replication and the cytopathic by viruses that encode helicase activity for their effect of BK virus in cell culture [8]. Type II topoiso- survival. The results presented here could be useful in merase, gyrase and topoisomerase IV are the cellular designing novel quinolone-related antiviral drugs. © 2007 International Medical Press 1359-6535 1 1_0131_Ali.qxp 1/2/07 12:36 Page 2 SH Ali et al. Materials and methods Results and discussion SV40 plaque assay Previous in vitro and in vivo studies have shown that CV1-P cells were routinely grown in Dulbecco’s quinolones can be effective against several DNA and minimum essential medium (Gibco BRL, Gaithersburg, RNA viruses [6,8,22]. To test if fluoroquinolones could MD, USA) with 10% Fetal Clone (Hyclone, Logan, UT, inhibit the growth of SV40, we tested four fluoro- USA). The cells were grown to 85% confluency in quinolones, namely, levofloxacin, trovafloxacin, 35-mm wells. The monolayer was then infected with ciprofloxacin and ofloxacin. SV40 was grown in CV1- SV40 for 3–5 h in complete medium containing 10% P cells in the absence or presence of these quinolones. Fetal Clone, using 20 μl of viral suspension per 35-mm All four quinolones tested were able to inhibit the cyto- well. The cells were fed on alternate days with fresh pathic effect of SV40 polyoma virus on CV1-P mono- complete medium containing the desired quinolone and layers in the viral plaque assay (Figure 1). allowed to grow for 8–10 days or until the cytopathic Comparatively, trovafloxacin proved to be the most effect (CPE) became visible. The monolayers were then potent inhibitor of the viral CPE, showing inhibition at fixed in ethanol, stained with crystal violet and 10 μM. levofloxacin was found to be inhibitory at photographed. 60 μM, whereas ciprofloxacin and ofloxacin inhibited the CPE at 1–2 mM. Quantitation of viral DNA To rule out the possibility that the four quinolone The monolayers of CV1-P cells were infected with drugs were inhibitory to the viral replication due to SV40 and incubated in the presence of a quinolone, their deleterious effects on CV1-P cells, the cells were as described above. The wells were fed every 48 h studied in the presence of these drugs alone. with complete medium containing the quinolone. Monolayers of CV1-P cells were grown for 5 days in The cells were lysed after various time intervals and the presence of the minimum concentration of each of the viral DNA was extracted using Qiagen miniprep the four quinolones that was found sufficient to inhibit columns (Qiagen, Inc., Valencia, CA, USA). The puri- viral DNA replication. Daily cell counts and cell-cycle fied viral DNA was then run on an agarose gel, trans- analysis by flow cytometry analysis (FACS) revealed ferred to a nitrocellulose membrane and Southern that CV1-P cells were unaffected by quinolone treat- blotted, using a 32P-labelled PfMl1-BsgI fragment ment. from the SV40 genome as probe. The DNA thus To study the effect of these drugs on the replication detected on Southern blots was then quantitated on of SV40 DNA, the experiment was set up in the same a phosphoimager. way as the SV40 plaque assay. Following SV40 infec- tion, viral DNA from the CV1-P cells was extracted at Helicase assay various time intervals and quantified on Southern The substrate was prepared by annealing the single- blots. All four drugs were found to inhibit the SV40 stranded, circular M13mp18 plasmid with a 32P- DNA replication (Figure 2). At 48 h, no effect of labelled 35-base-long oligonucleotide that was quinolones was observed on the DNA replication (data complementary to an M13p18 sequence. The not shown). After 72 or 96 h, however, a clear inhibi- substrate was purified using commercially available tion of the SV40 DNA replication by quinolones was columns and stored at 4°C. To perform the helicase observed. As with the CPE assay, trovafloxacin was assay, 0.5 μg purified T Ag (CHIMERx, Milwaukee, found to have the strongest inhibitory effect at 10 μM. WI, USA) was incubated with the substrate at 37°C Levofloxacin and ciprofloxacin showed a comparable for 45 min. The reaction mixture contained 40 mM level of inhibition, whereas ofloxacin was to found to Tris-HCl pH 7.6, 5 mM MgCl2, 2 mM dithiothreitol be the least potent of the four quinolones (Figure 2). In (DTT), 2 mM ATP, 25 mM KCl, 2% glycerol and the plaque assays and for the DNA replication experi- bovine serum albumin (BSA) (100 μg/ml). The heli- ments, the quinolone nalidixic acid was routinely used case assay was performed in the presence or absence as a positive control. Since nalidixic acid is known to of the quinolone. The reaction was stopped by inhibit the DNA replication of DNA tumour viruses adding a STOP buffer containing 50 mM ethylenedi- [8], we were able to successfully use it as a positive aminetetraacetic acid (EDTA), 2% sodium dodecyl control to validate our methodology (data not shown). sulphate (SDS), 40% glycerol and 0.1% To further investigate the mechanism of action of the bromophenol blue. The mixture was loaded onto an quinolones on SV40 replication, we sought to deter- 8% polyacrylamide gel containing 15% glycerol. The mine the effect of these drugs on the helicase activity of gel was allowed to run at 150 V, terminated after SV40 T Ag. In this assay, the ability of purified T Ag to 90 min, and dried. The signal was detected and release a 35-base complementary oligonucleotide from quantitated using a phospho-imager. M13mp18 plasmid was determined in the presence of 2 © 2007 International Medical Press 1_0131_Ali.qxp 1/2/07 12:36 Page 3 Fluoroquinolone inhibition of SV40 T helicase Figure 1. Inhibition of SV40 CPE by quinolones Levofloxacin mM 0.01 0.02 0.04 0.06 0.08 Ciprofloxacin mM 0.01 0.05 0.1 0.2 0.2 Ofloxacin mM 0.01 0.05 0.1 0.2 0.2 Trovafloxacin 0.02 mM 0.001 0.01 0.02 0.05 0.05 Virus + + + + – Monolayers of CV-1P cells were infected with Simian virus 40 (SV40) and incubated in the presence of the indicated concentrations of quinolones until cytopathic effect (CPE) was observed (approximately 10 days).

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