animals Article Possible Dietary Effects of Insect-Based Diets across Zebrafish (Danio rerio) Generations: A Multidisciplinary Study on the Larval Phase Matteo Zarantoniello 1 , Basilio Randazzo 1, Gloriana Cardinaletti 2 , Cristina Truzzi 1, Giulia Chemello 1, Paola Riolo 3 and Ike Olivotto 1,* 1 Dipartimento di Scienze della Vita e dell’Ambiente, Università Politecnica delle Marche, via Brecce Bianche, 60131 Ancona, Italy;
[email protected] (M.Z.);
[email protected] (B.R.);
[email protected] (C.T.);
[email protected] (G.C.) 2 Dipartimento di Scienze Agro-Alimentari, Ambientali e Animali (Di4A), Università di Udine, via Sondrio 2/A, 33100 Udine, Italy;
[email protected] 3 Dipartimento di Scienze Agrarie, Alimentari ed Ambientali, Università Politecnica delle Marche, via Brecce Bianche, 60131 Ancona, Italy;
[email protected] * Correspondence:
[email protected] Simple Summary: Fish meal and fish oil represent the optimal ingredients for aquafeed formulation. However, their partial or complete substitution with more sustainable alternatives, like insects, is required for a further development of the aquaculture sector. Nutritional programming through parental feeding may enhance the ability of the progeny to utilize insect-based diets. In the present study, five experimental diets characterized by increasing fish meal substitution levels with full-fat Black Soldier Fly (Hermetia illucens; BSF) prepupae meal (0%, 25%, 50%, 75% and 100%), used for Citation: Zarantoniello, M.; zebrafish broodstock rearing, were provided to the progeny (first filial generation, F1). The effects of Randazzo, B.; Cardinaletti, G.; Truzzi, BSF-based diets on F1 zebrafish larvae were investigated through a multidisciplinary approach.