AR TICLE Draft Genome Sequence of the Pine Pathogen Diplodia

ARTICLE 233 , # et ; 4 et al566_ # Key words: Pinus U U . ! 4 S V@ ! D. sapinea IMA FUNGUS · 6(1): 233–248 (2015) 233–248 · 6(1): FUNGUS IMA `/G 4F Pinus et al5667N /? U2 O 4 G@ ! U ` [ + 7 Leptographium lundber #!5 Diplodia scrobiculata Thielaviopsis punctulata and Thielaviopsis ] W / % % Z@/ S ; !"E#+ I@ O!""#@ O % .$ al!"""+ @ 9 [ et al56!! @ / + E Thielaviopsis punctulata < X * /+ 3 ! + @ E G `W ! ] Diplodia pinea !6 W //.> 5 > /% U " et al 566_ V V /< ! Chrysoporthe austroafricana, Diplodia scrobiculata, Fusarium nygami, Leptographium Chrysoporthe austroafricana, Diplodia scrobiculata, Q ; 2/ " D. sapinea !

Diplodia scrobiculata > X [ !! Chrysoporthe austroafricana V % % V

[ * <X 4 Q * / @ ; ; Z@ S% @ Z % N Z .G N*Z@/ < ; W X / @ VZ % U X _6!6/ @N %; @ ; ; I; .U + @ G Z % ]% >!774FZ@/ @ U U * Z@< / Z@+ >`6E"6!Z@/ < ] W / + N W/+NZ % ; ;+ 56; 665E@ / K V% V O 5FFU Article info:@ [!!U 56!4K/ [!F`56!4K; [55`56!4 77 U % ? $ % @ `; Abstract: ; GV O Z % @ S lundbergii, Limonomyces culmigenus, Stagonosporopsis tanaceti, lundbergii, Limonomyces culmigenus, Stagonosporopsis `V + U<56#64Z@/ G N*Z@/ ; 665E@ / . [ [^ O © 2015 International Mycological Association © 2015 International Mycological conditions: distribute and transmit the work, under the following are free to share - to copy, You Attribution: + < [ Chrysoporthe austroafricana, Diplodia austroafricana, of Chrysoporthe sequences Draft genome Limonomyces lundbergii, Leptographium nygamai, Fusarium scrobiculata, Stagonosporopsis tanaceti, culmigenus, IMA Genome-F 4 IMA !! !5 " !6 # E 4 F 5 3 7 ! Non-commercial:No derivative works:Any of the above conditions can be waived if you get For any reuse or distribution, you must make clear to others the license terms of this work, which can be found at http://creativecommons.org/licenses/by-nc-nd/3.0/legalcode. Nothing in this license impairs or restricts the author’s moral rights. permission from the copyright holder. Diplodia scrobiculata + Draft genome sequence of the pine pathogen IMA Genome-F 4A IMA [!644"E\ 56!46F6!!4 VOLUME 6 · NO. 1 \et al.

; % /Z]Z@VZ@ @ et al. > / + et al5667 566F !_F57 $ Diplodia scrobiculata O % D. D. sapinea_F"U Alternaria alternata__U % < scrobiculata > et al56!7 [\\ %\N

ARTICLE ? D. sapinea A. alternata!_7F" % % > et al56!7 V D. sapinea!_656/ D. scrobiculata ? Botryosphaeriaceae D. sapinea ; et al 56!_ % ? [ > 9 D. scrobiculata % % N $ than D. sapinea et al5665; ? D. scrobiculata ? SEQUENCED STRAIN South Africa: Mpumanlanga: @ $Pinus patula, Q 566E W. Bihon GU _655_ G+@!_"#"FK ; SU Authors[\!'?!@!\! F!57FM !!\

NUCLEOTIDE SEQUENCE ACCESSION IMA Genome-F 4B NUMBER

V]@ % Draft genome sequence of the <+`\SU+*\]+ */S]66666666 Eucalyptus canker pathogen V% % */S]6!666666 Chrysoporthe austroafricana

METHODS @ Chrysoporthe Cryphonectriaceae [566_ <>/ Diplodia scrobiculata Myrtales] O et al. 5667. GU_655_G+@!_"#"F $ ? Chrysoporthe austroafricana N [ 9 @? / . N? + N @ / Eucalyptus %Syzygium species ?? [et al. !"E"U et al.5665 $et al.5664 _6 ? 9 et al.566F> et al.566FX et al 56!!@ Eucalyptus G*G] de novo ? #6 O 466 G*G Q / < ] /Z]Z@VZ@ @ et al. 566F V Chrysoporthe C. cubensis Aspergillus oryzae [\\ . @ G / C. deuterocubensis . \ \ G / 9 et al. !"#F !"#" U et al 5665 % G S ]U et al.5664] O et al.566F% U et / GS]U/ ; et al. 566# G al56!6W % Chrysoporthe 1466 O[ C. doradensis S ] O et al. >G+N 5664C. syzygiicola C. zambiensis G et al.56!6 C. inopina C. hodgesianaG ] O et al.5667566F RESULTS AND DISCUSSION ; % Chrysoporthe % $ ] O et al. 5667 V ? D. scrobiculata > et al. 566#% U et al56!6 GU_655_G+@!_"#"F X et al.56!_ U O_4E4 U <% G*G et al5665 et al56649 et al566F> ] _#E" >46!F et al566FX et al56!!9% 2 $ O!!"#!4 $ % U % 57EG S ] GS] GS]U/ ; et al 566# % "45z ? ? C. austroafricana

234 IMA FUNGUS IMA Genome – F4

GU5!!_V % ARTICLE Chrysoporthe % [ V C. austroafricana Cryphonectriaceae O Neurospora crassa_""U !66E5K] et al SEQUENCED STRAIN 566_, Podospora anserina_FU !6474S et al566E Magnaporthe grisea76_U !!!6"K South Africa:2 U !"E"!¦§ < et al 5664 GU5!!_G+@!!5"!FK; SU4E65_M V % % NUCLEOTIDE SEQUENCE ACCESSION $ % V NUMBER Chrysoporthe % VC. austroafricana The Chrysoporthe austroafricana GU5!!_ ]@ % % ] @ ]+ Chrysoporthe `N;66666666 V % `N;6!666666 Authors[!?!|!!\?!@!\ E.T. Steenkamp, and N.A. van der Merwe

METHODS

] <>/ $ C. austroafricana IMA Genome-F 4C GU5!!_]? / G / G @ / N U @? . N G/ / Draft genome sequence of Fusarium <>/ ? . NV * V nygamai G G/ Z % ; V

? !66. The Fusarium fujikuroi $ WW@G %

? _66. G*G Gibberella fujikuroi $ ] % #4! G*G / < .U @?? ? / $ * I@ 566F U @? NV @@;/GS 2% et al. 566"V 56+ O 56!!; WW@G de novo /Z]Z@VZ@%_6_ Fusarium % F. verticillioides, F. circinatum, graminearum @ IU F. fujikuroi, F. mangiferae W G % 5664* @? ; [ et al. 56!5 et al. 56!_ V % O GS]U/%57 ; et al % 566# WW@G % % * I@ 566F2% et al.566"V RESULTS AND DISCUSSION $ $ W $ V C. austroafricana ? 77FF"!F" O V% 2% et al. 566" N F7!F 76 >46% . % % F!F5# W ]G WW@G % 4_"z/ GS]U/ % * I@ 566F2% "7_4 z 774 et al.566" V/Z]Z@VZ@ V WW@G F. dlamini, !_7E7 F. napiforme, F. nygami, F. acutatum, F. pseudoanthophilum, V C. austroafricana O F. udum, F. xylarioidesQR<et al. !""E5666 77FU Cryphonectria parasitica / F. udum, 7_"U K !! !E7 [\\ %\G 5 F. xylarioides+ !"#! F. nagamai % \G 5 $ % V Cryphonectriaceae % ? F. nagamai. The species V% [ .

VOLUME 6 · NO. 1 235 \et al.

$2 I>!""F RESULTS AND DISCUSSION [ V % / Fusarium nygamai @ et al. !""4 O4!F!465" % % Fusarium nygami ___$V 76"

ARTICLE O 566 4_!"65_ >46 V I+ !"E_ % 5#F!F4F % ]G 7#7FzQ 76" !4 !666666 O * I @ 566F O $ #Ez Q% ? ?+ $ % WW@G ; et al. 566# F. nygamai $ "F_#z ""z % $ % VF. nygamai is !4#E6 %Q W V F. nygamai F. SEQUENCED STRAIN verticillioides 7!E U F. circinatum 77_ U F. fujikuroi 7_" U F. mangiferae 74F U et al. USA: Pennsylvania[ $ 56!_ V O % F. F. nygamai U.!4F7 $ U.5_#6 solani 4!_ U et al. 56!_ @ / / U/V.! Q W F. solani ] 2 WW@G I > !""F ` !""F J.A. KlaasenIP.E. Nelson et al. 56!_ ; +*/@V U GE47F GU!5E7# 2@Z ].64!!! GUW!56" the F. nygamai ? U.#7"52.!7E; SUMF!5## % V Fusarium F. circinatum % NUCLEOTIDE SEQUENCE ACCESSION > et al. 56!7 V % NUMBER WW@G The Fusarium nygamai ? % % $ <<+`\SU+*\]+ *+> 66666666V[ % WW@G Authors[ Q.S. Santana, G. Fourie, L. de Vos, !!?$?!@!\ METHODS !!\!+!]

] <>/ !.. . $ IMA Genome-F 4D ;

236 IMA FUNGUS IMA Genome – F4

VLeptographium s.lat. 9UU[ ARTICLE % ? O ]U N ; @ %I/ V Grosmannia clavigera 566! Leptographium procerum < ] et al. 56!! % ; / > et al56!7V % ? V L. lundbergii GS]U/; et al.566#566" .% +Z@GQ@ et al56!4V . ? [ O % + V <et al56!_V et al56!_ L. lundbergii Leptographium s. lat RESULTS AND DISCUSSION

SEQUENCED STRAIN / 55 % E74 ? [ <.% Norway: Pinus sylvestris !"E" H. Roll-Hansen G*G ] GU5!"6G+@!_E#!FK; SUF!5#EM 7!5 % 466 OV>46% 5!5 2 % !U V% #6 V NUCLEOTIDE SEQUENCE ACCESSION OL. lundbergii 5FFU NUMBER L. procerum5EFU G. clavigera5"E U < ] et al 56!! % > et al 56!7 V V ?Leptographium lundbergiiGU ]G L. lundbergii 4FE z 5!"6 G+@ !_E#!F <<+`\SU+*\ G. clavigera4_7z ]+ */ $ [!7_EG[ <[ ~W[ U[ L. lundbergii GU 5!"6 G+@ !_E#!F [V GS]U/ +Z@GQ < et al 56!_ ] <>/ % R N 9 ?? ] U/2S "47# G Z % G < % G/ V U/2S R $ E6_4 _46 4_6 % O \ ; / % U/2S R !66 . ? G et al. 56!7 Leptographium lundbergii N 9 @?5666 ? !\5#E ]G [ !4 ? 47z 5#4z $ #65 4R <.% zQ U/2S 56#6 [ G*G $5!#z #7## .$ %E6!G*G+ / < V? #E_zV $ 477 !5F GS]U/ ; et al.566#V V?L. lundbergii MAT1- U/2S 2 MAT1-2-1 G et al 566E % MAT1-1-1 V MAT1-2 W @ ; et al5664 O % V Leptographium s. lat. < et al 56!_ V U @ et et al.56!_V L. lundbergii represents a al56!_M!4W @>/;2 % 5667 / @ I 566_ ]U * I+ %!""E Leptographium / V . ?L. lundbergii @>/; Grossmania clavigera < ] et % al56!!Ophiostoma ulmi 2 et al.56!_O. novo- ulmi G et al56!4O. piceae9 et al.56!_ species in the Ophiostomatales Magnaporthe grisea < et al. 5664 Neurospora crassa] et al566_ % Authors[+!!?!@!\!\! V M. grisea / !!\

VOLUME 6 · NO. 1 237 \et al.

IMA Genome-F 4E % K W /G56!_ Draft genome sequence of N % S. tanaceti

ARTICLE Stagonosporopsis tanaceti O ? [ V Stagonosporopsis Didymellaceae / de novo S. tanaceti ] et al.566" % % S. cucurbitacearumDidymella % bryoniae S. andigena S. crystalliniformisK % ? %/% et al.56!6] et al.56!5Stagonosporopsis tanaceti SEQUENCED STRAINS Phoma ligulicola% inoxydabilis Australia[ Tasmania[ V @ Tanacetum cinerariifolium / ; I Tanacetum cinerariifolium 5667 S.J. !""E X [ et al. 56!5 V [ PethybridgeG+@9.56"7#M KG+@!_!7E7M / [ !""4 $. K T. cinerariifolium5667S.J. Pethybridge % G+@!_!7E4K T. cinerariifolium5667S.J. Pethybridge !""" !66z; et al. V/@4446_K T. cinerariifolium!""ES.J. Pethybridge 566E V S. tanaceti is / ? ; et N !66 . al. 566E @ % % S * @ S* Z % Stagonosporopsis tanaceti % ] ]/ V 5" _"4 #!F 4E] V %655 [\\ % % \\ ? % 9% 7. ? % _6 _6 / 5!44_E_7 S. tanaceti U #__5z 44!"#7E !E#E Dothideomycetes $ z ? #" . [ . [ z ; < I S 5666 W et al. 566E N @Q/;% % !64 * et al. 566E S. chrysanthemi $ 5! 4! 5 @ I + !"#7 V $ >46 !"#4 % _4V in the S. tanaceti- /% G S ] U % / GS]U/ % !6 ; et al 566# ] U %5_!#G et al. S. tanaceti 566EK ab initio % . U % ? % % % % Q % 1466 < / O ab initio / [ % @>/;2 5667 / %544@ ; et al. 5664 566E 9% et al. 566F % .

238 IMA FUNGUS IMA Genome – F4

S@V ?Didymella exigua <>/ Laetisaria Limonomyces appear ARTICLE [\\ . \< $!\< $! ? V < et al.56!! . %.9UU [ S. tanaceti, which U Limonomyces [ @>/; % Agrostis, Cynodon, V [ 9UU [ [ Festuca, Lolium, Poa @ et al. 5664V G et al.566E [ L. roseipellis L. culmigenus @ I * !"E5 Limonomyces roseipellis L. culmigenus $ L. RESULTS AND DISCUSSION culmigenus% % @ I* !"E5N V O76EU % L. roseipellis % !66&>46% "E!4# Laetisaria fuciformis@ et al.5664+ L. roseipellis $ O!!5F6# / Laetisaria fuciformis 1566 "E_7 %% >G+NU % %@ et 57EG S ]GS] GS]U/; et al.5664N al566# "7z % % 5_7GS]"7_4z 577 "E_" z Ab % +et al.566F initio @>/; [!#5!" Z L. roseipellis Laetisaria fuciformis Q W Q W Limonomyces culmigenus V O S. tanaceti 76E U % S O Dothideomycetes, G [% V Pyrenophora teres7!"4U Zymoseptoria tritici Dactylis glomerata _"#U Stagonospora nodorum_#5U 9 et al.566#Set al.56!6] et al. 56!!V @ I * !"E5 Didymella exigua, which L. culmigenus O_7_"U [\\ . \ % < $!\< $! % W I 56!4 V S. tanaceti V . X [et al.56!4 L. culmigenusG+@FF!E4 % X [et al. 56!4 V % [ V [ Corticiaceae Stagonosporopsis % . . SEQUENCED STRAIN

Authors[!Q'!!!@!'= Canada: British Columbia[ and P.W.J. Taylor !"E4J.R. BandoniG+@FF!E4K+;NE"_!"_M

IMA Genome-F 4F NUCLEOTIDE ACCESSION NUMBERS

V ] @ G+@ FF!E4 Draft genome sequence of the >G+N ]+ *GV66666666 % *GV66666666 fungus Limonomyces culmigenus > ? 9Corticiales are an MIP % % >G+N]+ 2 ##""!4%2 ##""55 * et al. 566E V . Corticiales, Limonomyces Laetisaria, METHODS % % @ I* !"E5U ] <>/ $ [ % Limonomyces U . et al.56!4 Laetisaria @ I * !"E5 9% Limonomyces culmigenus G+@ FF!E4/ N V @? ;G .W <>/? N @ < G/

VOLUME 6 · NO. 1 239 \et al.

? [ 56\ _F4*46#6FV % % 5!!. * V ] N > * G V <>/ ]G 4#EzV O #46G * @ 9 U/ ? EEE" 466 N U @? F66.? 546#4# %76F4 V N N OG+@FF!E4

ARTICLE G*G] %#F! G*G ? Agaricomycetes + + U// % Schizophyllum commune _E4 U K Q et al. 56!6, C. %? ?; ? ¢664 cinerea _# U K @ et al. 56!6 Phanerochaete !4 F66 / de chrysosporium _4!U KU Oet al.5667V novo? + $ "!4z F! O46 {466 . % / ; et al.566#V % G*G ] ; N>@S % 6567 % G+@FF!E4 @ S 56!! Z/@V ! ] % % et al. 56!_ V / % GS]U/ ; et al. L. culmigenus G+@ FF!E4 566# ;*/>V < % S% [\\ % \\ _F! U /Z]Z@VZ@ % _65 Coprinopsis % OV cinerea @ et al. 566E N [ G+@FF!E4 Q UG* * L. culmigenus et al. 566_ % % % @ I* !"E5 % G*G X [ "_4 O ] % % "5F z K @>; !6$ % {"6z F#7 #Fz @ O . % / [ % +*/@V {!666 ![! % % [ !66 % . [ % % K !66 % [ ? % ; % % !#_7$ versus % ];N. [ @SG SVQQ*G O et al.56!7; % % % E_7$< [ @U/@9+ et al. 56!_ G/ G/> ? et al.56!5 L. culmigenus V / . Agaricomycotina ` % ]N U]; [\\ 9/ 45_7 FE [ G+@ FF!E4 _E6z %V/ + >/ .@S % !5! * I S !""# % O @ et al. 5664 V R. solani !/; % V L. culmigenus UZU %_5_ 2 O et al. 5667/ +*/@V G+@FF!E4 S.% !6S.4 / !F_"7 % [ L. culmigenus G+@FF!E4 % !!_" RESULTS AND DISCUSSION 747\U ; !F%F5F5 %_#"G V Limonomyces culmigenus %

G+@FF!E4 _F!U _6E7_5F7 . K>46"

240 IMA FUNGUS IMA Genome – F4

Table 1.@ ?Limonomyces culmigenusG+@FF!E4 ? ARTICLE

@ et al.56!6W et al.56!5 et al.56!_ Species Limonomyces culmigenus Rhizoctonia solani Tremella mesenterica Coprinopsis cinerea CBS 661.85 AG-1 1B Fries v1.0 7#130

Summary Data V ? U _F! 7## 5EF _#4 > EEE" !F66 74 !_ ]Gz 4#E 7E! 74# 4!# ; !F_"7 !4!4# E_!_ !__"_ ; !65# E#F 5!_ ##F Predicted CAZymes V G/ E6" "77 5E5 FF! /$ % EF !_5 "4 !64 G !54 !_6 !5 !55 G !5E !7! " !!4 ] _44 _7E #E 5!5 ] !6F !6! E7 E" ; " "5 7 !E Predicted Secondary Metabolite Clusters V @UG 5_ !_ 7 !7 VN ;2@ 3 6 6 ! VNNN;2@ 5 6 6 6 > ! 5 6 ! V E E 3 " 9 6 6 6 6

L. culmigenus Agaricomycetes; Laccaria / Agaricomycotina % bicolor, Coprinopsis cinerea Schizophyllun commune Rhizoctonia solani 56 F!7 !_ _75 !_ 5!6 Tremella mesenterica % @ et al. 56!6 Q Coprinopsis cinerea, the L. culmigenus G+@FF!E4 [ #4Fz L. culmigenus %% ; % [ . [% V !V E6" [ V !65# G/ G+@FF!E4G L. culmigenus G+@ G/ [ L. culmigenus FF!E4 Ez Agaricomycotina L. culmigenus ; ? G/ _F%!E__ %_664 R. solaniN L. culmigenus F6! [ @@;K R. solani % $ 0_66 !54 . ]9 % % 14zK@@G;@% !4$M!F$K V ! V ]9 .@@G; % ]9 % % W . $ / $ @@G; 5_ O O ;* L. culmigenus V ;* $. .!_. R. solani /].!!+ "5;* V V ;* E#@@G; % R. solani /].!!+ +*/@V >G+N R. solaniR ]+ V . V L. culmigenus G+@FF!E4 4E . [ #5 F#7 5M" ]G 5#z % % _

VOLUME 6 · NO. 1 241 \et al.

464$+ >/ % . V . @ et al566!/.> et al56!7 O * I % >/ [ @ !""" 2 et al!""_U 56 V >/ [% !""5 @ et al 566! / ;@ 5 >/K*U _ Z@/ @ / + !"7! * I @

ARTICLE >/ V !7 % !"""/ + I* 5664 [ 2 !""_ /V; U O I U 5664 V . ATP6, ATP8, ATP9 COB [et al!""_ $ COX1, COX2, COX3 % %>/<9 NAD1, NAD2, @ et al566! NAD3, NAD4, NAD4L, NAD5, NAD6/ R. / [ Thielaviopsis paradoxa as the solani !/; >/ / I / * et al. 56!7K 566_N56!_T. punctulata . $ NAD1 $ >/Q% 4_ [ /.> et al !7 % 56!7 V % ]NN] % */]*N/ !_ G T. paradoxa L. culmigenus G+@ FF!E4 T. punctulata #"M!66 z T. paradoxa / in R. solani !/; * et al. 56!7 9% et al566" V ? N % G <;! T. punctulata L. culmigenus G+@ de novo N FF!E4 % % ? ? % _5 %% >G+N ]+ [\\ %\\ $ V Q ]/>S**S@\ 64\64\56!4, with % O % #55 ]G % 5E_ >/ %574 >/ %_5F SEQUENCED STRAIN V ? L. culmigenus G+@ FF!E4 [ Corticiaceae Qatar: < $Phoenix dactylifers U % V % % 56!_F.A. Al-Naemi+;NE"_!#_M ? $ L. culmigenus Corticiaceae NUCLEOTIDE SEQUENCE ACCESSION % NUMBER

Authors[M. Malapi-Wight, L.A. Beirn, D. Veltri, V ]@ and J.A. Crouch <<+`\SU+*\]+ */SX66666666 V% % */SX6!666666

IMA Genome-F 4G METHODS

Draft genome sequence and G <;! % Thielaviopsis punctulata, was annotation of Thielaviopsis $ <>/ GV/+ I 2 !""6 V @? . punctulata, the causal agent of >$ . . black scorch disease in date palm ? 9 @?5466 !46 V ? < Phoenix dactylifers V + et al56!7K 76 @;/+ % et al56!5 % de novo GS]U/; >% et al566# . % T. % punctulataU/2S %5_!#G et al566Eab initio Thielaviopsis punctulata % Ceratocystis radicola % VU/2S %5_!#G et al566Eab initio gene

242 IMA FUNGUS IMA Genome – F4

>;;! . ARTICLE GS]U/ ; et al \ $ 566# @>/; ` et al 566E /Z]Z@VZ@ @ et al5667]U + I+ %5664 % T. U @ et al56!6W [ punctulata % U/2S GS]U/ Ceratocystis s. lat. UZU @>/; U/2S V Z/@V . % [ V " @ ; >G+N ]@ /;266666666! et al U/2S W 56!_ C. manginecans ]@ `` 66666666! U/2S [ + et al 56!7 Huntiella moliniformis ]@ @>/; /Z]Z@VZ@ @ ; K `U@966666666!+ et al56!7V [ [ U/2S [ % #4 @ ? U/2S z T. punctulataG <;! % Ceratocystis Huntiella ?N [ U/2S % % % / [ N ; @ % 47E ` et al 56!7 +*/@V; % Ceratocystis 555EG et al566" >G+N. @? Huntiella % et al56!_ F"#` 56!4; et al56!5 + et al56!7 UZU 2 O et al V T. punctulata G <;! 5667 Z/@V%5_] % et al56!_ % % T. punctulata V . % T. Ceratocystis s. lat[Huntiella moniliformis% punctulata % > et al56!7" et al56!_ C. % manginecans% > et al56!7

Authors[ O. Radwan, F.A. Al-Naemi, G. Rendon, RESULTS AND DISCUSSION and C.J. Fields

V 7E6 % O . 4M# ? 9 @?5466 ACKNOWLEDGEMENTS ? 5_ "7F "F# 7!"#566E %V V ? Diplodia scrobiculata, Chrysoporthe 5E!U #4$?% austroafricana, Fusarium nygamai, Leptographium lundbergii . {!"" ] N Z % ; >G+NR V V ; G. %; V;G; <@V.> W 5_#" 566 >46"5#5_ G S$ V 9 + GV9+ *46E# W / + N W/+N V T. punctulata > W > W Z % GS]U/ T. punctulata ; ; @ / + G [577 57E . % *W @ / ; .< W V in T. punctulata"E_Ez U/2S %5_!# Stagonosporopsis tanaceti + 47E6 ab initio / / @ % ; * Z@NW/ N ; @ 9 >].F54757 V> / 7E7! K +*/@V; 5#7" S$ @ G Z % ]% > [ ? $ % V ? Limonomyces culmigenus G+@ !6$!6._ ]Q N @ FF!E4 Z@< / Z@/ /V; % Z@

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ARTICLE V Thielaviopsis punctulata + /U * U Z + 56!7 V [ \$ > W> W N ? Bioinformatics[ !6!6"_\ > ; ; >; ;.4.!665.7.6!6 N \ !#6 + @/ G ++ U / 566F + [ % > W /% . Annual Review 9 O `G % + G \U2G of Phytopathology 44[5!_M5_7 Z % N Z .G N* + G !"#! The Genus Fusarium 2[ G ? U N / @ < > + GS * +W 5664 W % [ S% @ Z % N Z .G % Current Opinion in Microbiology 8[ N* _F5M_F" + V] V [U` [+<5667] Diplodia scrobiculata % REFERENCES Pinus radiataMycological Research 108[!_""M!76F G G G ] /% X U > ; ` / S9/ /@566_W et al. 566" +*/@V[ BMC Plant Pathology 52[#"6 Bioinformatics 15[!6[75! / @2/ *U S]O.X @@ èt al G U@9 GU + U56!7] 566"N Thielaviopsis U/2S U/2S ‐; Current Protocols in paradoxa Tpunctulata Bioinformatics 48[7!!!.7!!_" S$ . @ Journal of Plant Protection Research 49[ G +*2 N @U; ] Set al.566EU/2S [ 5#FM5#" . . /.> W/> /V/ Q56!7W Genome Research 18[!EEM!"F Thielaviopsis punctulata G < ] O U U .2 / [ Plant Disease Journal 98[!7_# U` $ ` 56!6 V $ % /. U+RG UU/.U /U< U*]@ Chrysoporthe Eucalyptus grandis Syzygium 56!!W Ceratocystis radicicola guineense Mycological Progress 9[_#"M_"_ Q New Disease Reports 23[5_ G /U< `+% ]W` X> Uet al.56!4 /% U<] ` `X ]G ; W Ophiostoma novo-ulmi [ 56!69 Didymellaceae[/ < Phoma ; Genome Biology and Evolution 7[7!6M7_6 Studies in Mycology 65[!MF6 G O / / /U / U Q O `/ * % `* 56!7 + % / > @ / % < ] % // <% U et al @SG SVQQ*[ 56!5 @;/[ Amino Acids 46[7#!M7#_ .? Journal of Computational < USS U5666V % Cercospora $ Biology 19[744M7## [ + U < / ` W S$ W W O G et al. Annual Review of Phytopathology 38[7F!M7"6 56!_ UNVQ@[ N % de novo O + <V/+ N [+< [U`56!7 Molecular Phylogenetics and Evolution 69[ [ Ceratocystis Studies in Mycology _!_M_!" 79[!E#M5!" + `, + %U5664]U [ + [ U` 56!_ S [ % Nucleic Acids Ophiostomatales N[ The Ophiostomatoid Fungi: expanding Research 33[74!M747 frontiers @ 2/ + [ U` [ 5!M7F + @ ++ V [U` [+<56!! G+@+ % @ !5~Z [[email protected]>/W Diplodia scrobiculata @ 9 Forest + % G Pathology 41[!#4M!E! ] ` /% UU `9G X ]Ù + 2 U U9 2 O < W U/ + ] èt al566"U Phoma V S et al.56!_ @U/@956. % [ [ Phoma Nucleic $Mycological Research 113[46EM4!" Acids Research 41[567M5!5 ] ` + ] X < / 9 5665 G +

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2 `/>;S!""FN [ > ] $ ` ] O U [ U` 566F Gibberella nygamai % Fusarium nygamai < Chrysoporthe Eucalyptus Mycologia 88["F4M"F" Syzygium / Plant Disease 2 N5667][ %BMC Bioinformatics Reporter 90[#_7M#76 5[4" QR<2G S> 9N!""EU

ARTICLE 2 O@; /< /*@ U@ Uet al.5667 Gibberella fujikuroi $ X Mycologia 90[7F4M7"_ Genome Biology 5: !5 QR< 2 > 9N/ VG S 5666/ 2% U U WQ [ +< [ U` @ Gibberella fujikuroi $[ SV 566" < % % Fusarium species in the Mycoscience 41[ Gibberella fujikuroi$Fungal Diversity 34[!M5! F!M#E * V * ] U S !"5# + Q /``W**]/ /2 Set al.56!6 Svenska ] ? Schizophyllum Skogsvårdsföreningens Tidskrift 25[!74M5#5 communeNature Biotechnology 28["4#M"!6 * `W@ +/566FThe Fusarium Laboratory Manual ; U/ @ S* [ U` !"E# X *[+ ; Sphaeropsis sapinea Z * N* 2 2 `566E@Q/;[ @ Phytopathology 77["77M"7E Bioinformatics 24[#!_M#!7 ; ]+ 22 N566#GS]U/[ * *@ G` <@566_Q UG*[N [ Bioinformatics 23[ Genome Research 13[5!#EM5!E" !6F!M!6F# * G@ /!"""W O Ceratocystis ; ]+ 2> 2 V2 N566"/ radicicola @ / Plant Disease 83[EE6 Nucleic Acids Research 37[5E"M * + <V/> ;; /et 5"# al.56!5Grosmannia Leptographium ; @ G!""EG[ . W / Australasian Plant Pathology 27[74M7E Leptographium taigense % Antonie Van Leeuwenhoek ; @` 9 W@ S ;< ] <9 G et al. 102[_#4M_"" 566E < V [ * *; @+W % ><` X@ @/et al. Plant Disease 92[!5F6M!5#5 56!7 U $ ; @`9 W@ G ] V5664<% Rhizoctonia solani /]._ . FEMS Microbiology Letters 352[!F4M!#_ Phoma ligulicola V [Plant *VUS@ !""# >/ .@S[/ % Disease 89[!!!7M56 >/ ?Nucleic ; @` @ `+ 9 W@ 56!5 * % Acids Research 25["44M"F7 Phoma ligulicola % * /X+ %U!""E]U [ inoxydabilis / [Plant [ Nucleic Acids Research 26[!!6#M!!!4 Disease 96[#7FM#4! U . U@ .@ O G< `X <G `/ ; /`*/%// O `@ + [U`et 56!4< ?Dactylonectria macrodidyma, al.56!_VBotryosphaeriaceae[ Nectriaceae Genome Studies in Mycology 76[4!M!F# Announcements 3[ 665#E.!4 ; /* ` >G ;%O ;/ 5664 De novo [ U O < * *W ; > ] U<@ 9 2 et Bioinformatics 21[ _4!M _4E al. 5667 ] ? ; 2<V % V+ ] U < 56!5>G+N Phanerochaete chrysosporium ;#E Nature @? @?[ Biotechnology 22[F"4M#66 Nucleic Acids Research 40[ [;; / South U 9] O U9 $` [+< [ African Journal of Science 101[!M4 U`5665G Tibouchina @ / @ /S+ *+ <<` U!""4 Mycological Research 106[!5""M!_6F U Fusarium U 9 ] O U [ +< [ U` 5665 / Mycological Research !.V 9_ ? 99[45_M45E Cryphonectria cubensis @ / / @ @ <* + ]] O U [+< [U` $` @ <* +

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@ ; + /> * VU 5664 V >/ .@S X [>/;29 W@; @`W et al.56!4 ARTICLE ];@ % >/ N [ MAT1 Stagonosporopsis tanaceti, >/Nucleic Acids Research 33:FEFMFE" $ $ / @ S 56!! [Fungal Biology 119[76EM7!" Bioinformatics 27:EF_MEF7 X [>9 W@/;; @W et al.56!4 @ W/ UN ;2 % % SX % Stagonosporopsis SU56!4+Z@GQ[ tanaceti / [Phytopathology . Bioinformatics 105[_4EM_F" [!6!6"_\ \ %_4! X [> ; @` W > US G ; et al. @ `V 2 ` @< @ `S ` @`U + 56!5Stagonosporopsis 566"/+@@[/ ? AsteraceaeAustralasian Plant Pathology 41[F#4MFEF Genome Research 19[!!!#M!!5_ % / 9/ > US ] ` !""6 @ @ /W/9 ] ;!""FM56!6 U Q._6 Studies in [\\ Mycology 32[_M!" @ /W/9 ] ;56!_M56!4 U Q. % U >/] O U@ SV [+< 76 [\\ [ U` 56!6 U @ `S @2 / < / G9 + + et al. 56!6 [ $ N % within Chrysoporthe cubensisFungal Biology 114["FF."#" Coprinopsis cinereaCoprinus % > U + */ G `/ < `S < + cinereus Proceedings of the National Academy of Sciences, et al.56!7 < Amanita jacksonii, Ceratocystis USA 107[!!EE"M!!E"7 albifundus, Fusarium circinatum, Huntiella omanensis, @ `/ * U !"E5 Laetisaria Limonomyces Leptographium procerum, Rutstroemia sydowiana Corticiaceae Sclerotinia echinophila. IMA Fungus 5[7#_M7EF Canadian Journal of Botany 60[45"M4_# % > U/ + X * > 2 < et al @ U< U+ 9 <566EZ % 56!7 < ? Diplodia sapinea, <>/ %% Ceratocystis manginecans, Ceratocystis moniliformis IMA [ Bioinformatics 24[F_#MF77 Fungus 5[!_4M!76 @ UU +5664/Z]Z@VZ@[ % X U ] O U [ U` $ ` 56!! > .[ Cryphonectriaceae / Nucleic Acids Research 33[7F4.7F# Latruncellus aurorae%Mycologia 103[447M4F" @ U @ Q U + @ 566F X U] O U [U` $`56!_; ] O Chrysoporthe austroafricana / U % $ BMC X % G ] XG] Bioinformatics 7[F5 Forest Pathology 43[!57M!_! @ U @ , @, U + 5667 / $ % /Z]Z@VZ@[ % [ Molecular Biology Reports 8[!E6M!E4 Nucleic Acids Research 32[_6"M_!5 <`* Q9 US Wet al. 56!_ @ U@ 566_] U % S ? Bioinformatics 19[ 5!4M 554 Rhizoctonia solani/]!.N+ @ O] @ `@ < !""" /;< O Journal of Biotechnology 167[!75M!44 O Sphaeropsis sapinea % ;@ GU2%+ 2@ *> S.U Mycological Research 103[!!"_M!565 et al. 56!_ < [ . @ %W*!"6#V Botanical Gazette Fusarium fujikuroi % $ 44[57!M54E % PloS @ ;/.U /UOG/566!V Pathogens 9[!66_7#4 Chalara ;U@ SV<+ [U` [+< paradoxa Chalara radicicola Plant Disease 56!_< ? 85[E6ME_ "IMA Fungus 4[_4#M_4E @W+ U@ >@V56!! SXN]Q O [ +< @ SV@ G O U;/ + @ % O PLoS One 6: et al. 56!5 W ? / [ / 5!E66 South African Journal of Science 108[!M5 V]Q G 5666] [ [U`566_N $ [ Nature Genetics 25[54M5" @ 9 [ G V <@+ *!"E_W Australasian Plant Pathology 32[!__M!_" @ Plant [U`@ 2/ `W!""_) Ceratocystis and Protection Quarterly 1[_M" Ophiostoma: taxonomy, ecology and pathogenicity.@ ; U>[ V G2U < ] @ W > < + et al. 56!_ / ; @ ; Z? % . MAT [U`@ `/ +`!"E"W G Grosmannia clavigera % G Eucalyptus @ / Phytophylactica 20[_!!M ]_[Genes|Genomes|Genetics 3[7F4M7E6 _!_

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U `GU Wet al.56!5 G/>[ % SU / 566! N ; @ . %O . N ; Nucleic Acids Research 40[774M74! Bioinformatics 17[E7#ME7E ARTICLE

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