IMA FUNGUS · 6(1): 233–248 (2015) [!644"E\ 56!46F6!!4 IMA Genome-F 4 ARTICLE Draft genome sequences of Chrysoporthe austroafricana, Diplodia scrobiculata, Fusarium nygamai, Leptographium lundbergii, Limonomyces culmigenus, Stagonosporopsis tanaceti, and Thielaviopsis punctulata + < [!; 2/5W //.> 3* /+ 7 + 4F`/G # + 4* <X!V /<!G `W E] W 4/? U2 O !U U . #, @ `; "Q !6] E+ @ ! G@ !S V@ 4; `V !!> X ["> /% U !< X #!5 U ` [4 !< ] W / + N W/+NZ % ; ;+ 56; 665E@ / K . [ [^ O 5< W S @ VZ % U X _6!6/ 3< + S% @ G/ @ Z % < 7< ; + ; Z % 4"< >+ >`6E"6!Z@/ 4< U ; ; W / + N W/+NZ % ; ; $56 ; 665E@ / F/ G X Q ; N ;+ 5"_; 666!@ / #@ U U * Z@< / Z@<// @ % /@+ % U<56#64Z@/ E9 ; + G ] ` G % + G \U 2 G Z % N Z . G N*Z@/ "@N %; @ ; ; I; .U + @ G Z % ]% >!774FZ@/ !6< > S% @ Z % N Z .G N*Z@/ < ; ; GV O Z % @ S !!W X / @ VZ % U X _6!6/ !5Q * /@ ; ; Z@</./@+ % U<56#64Z@/ Abstract: VChrysoporthe austroafricana, Diplodia scrobiculata, Fusarium nygami, Leptographium Key words: lundbergii, Limonomyces culmigenus, Stagonosporopsis tanaceti, Thielaviopsis punctulata V% V O 5FFU Leptographium lundber 77 U Chrysoporthe austroafricana V % % % ? $ % Article info:@ [!!U 56!4K/ [!F`56!4K; [55`56!4 IMA Genome-F 4A Pinus Z@/ S ; !"E#+ I@ O!""#@ Oet Draft genome sequence of the pine al!"""+ et al5667N @ 9 [ pathogen Diplodia scrobiculata @ / + et al56!! Diplodia scrobiculata Diplodia scrobiculata % Pinus + D. sapinea Diplodia pinea .$ D. sapinea et al566_ / et al 566_ V % % © 2015 International Mycological Association You are free to share - to copy, distribute and transmit the work, under the following conditions: Attribution: [ Non-commercial: No derivative works: For any reuse or distribution, you must make clear to others the license terms of this work, which can be found at http://creativecommons.org/licenses/by-nc-nd/3.0/legalcode. Any of the above conditions can be waived if you get permission from the copyright holder. Nothing in this license impairs or restricts the author’s moral rights. VOLUME 6 · NO. 1 233 \[et al. ; % /Z]Z@VZ@ @ et al. > / + et al5667 566F !_F57 $ Diplodia scrobiculata O % D. D. sapinea_F"U Alternaria alternata__U % < scrobiculata > et al56!7 [\\ %\N ARTICLE ? D. sapinea A. alternata!_7F" % % > et al56!7 V D. sapinea!_656/ D. scrobiculata ? Botryosphaeriaceae D. sapinea ; et al 56!_ % ? [ > 9 D. scrobiculata % % N $ than D. sapinea et al5665; ? D. scrobiculata ? SEQUENCED STRAIN South Africa: Mpumanlanga: @ $Pinus patula, Q 566E W. Bihon GU _655_ G+@!_"#"FK ;SU Authors[\!'?!@!\! F!57FM !!\[ NUCLEOTIDE SEQUENCE ACCESSION IMA Genome-F 4B NUMBER V]@ % Draft genome sequence of the <+`\SU+*\]+ */S]66666666 Eucalyptus canker pathogen V% % */S]6!666666 Chrysoporthe austroafricana METHODS @ Chrysoporthe Cryphonectriaceae [566_ <>/ Diplodia scrobiculata Myrtales] O et al. 5667. GU_655_G+@!_"#"F $ ? Chrysoporthe austroafricana N [ 9 @? / . N? + N @ / Eucalyptus %Syzygium species ?? [et al. !"E"U et al.5665$et al.5664 _6 ? 9 et al.566F> et al.566FX et al 56!!@ Eucalyptus G*G] de novo ? #6 O 466 G*G Q / < ] /Z]Z@VZ@ @ et al. 566F V Chrysoporthe C. cubensis Aspergillus oryzae [\\ . @ G / C. deuterocubensis . \ \ G / 9 et al. !"#F !"#" U et al 5665 % G S ]U et al.5664] O et al.566F% U et / GS]U/ ; et al. 566# G al56!6W % Chrysoporthe 1466 O[ C. doradensis S ] O et al. >G+N 5664C. syzygiicola C. zambiensis G et al.56!6 C. inopina C. hodgesianaG ] O et al.5667566F RESULTS AND DISCUSSION ; % Chrysoporthe % $ ] O et al. 5667 V ? D. scrobiculata > et al. 566#% U et al56!6 GU_655_G+@!_"#"F X et al.56!_ U O_4E4 U <% G*G et al5665 et al56649 et al566F> ] _#E" >46!F et al566FX et al56!!9% 2 $ O!!"#!4 $ % U % 57EG S ] GS] GS]U/ ; et al 566# % "45z ? ? C. austroafricana 234 IMA FUNGUS IMA Genome – F4 GU5!!_V % ARTICLE Chrysoporthe % [ V C. austroafricana Cryphonectriaceae O Neurospora crassa_""U !66E5K] et al SEQUENCED STRAIN 566_, Podospora anserina_FU !6474S et al566E Magnaporthe grisea76_U !!!6"K South Africa:2 U !"E"!¦§[ < et al 5664 GU5!!_G+@!!5"!FK;SU4E65_M V % % NUCLEOTIDE SEQUENCE ACCESSION $ % V NUMBER Chrysoporthe % VC. austroafricana The Chrysoporthe austroafricana GU5!!_ ]@ % % ] @ ]+ Chrysoporthe `N;66666666 V % `N;6!666666 Authors[!?!|!!\?!@!\[ E.T. Steenkamp, and N.A. van der Merwe METHODS ] <>/ $ C. austroafricana IMA Genome-F 4C GU5!!_]? / G /G @ / N U @? . N G/ / Draft genome sequence of Fusarium <>/ ? . NV * V nygamai G G/ Z % ; V ? !66. The Fusarium fujikuroi $ WW@G % ? _66. G*G Gibberella fujikuroi $ ] % #4! G*G / < .U @?? ? / $ * I@ 566F U @? NV @@;/GS 2% et al. 566"V 56+ O 56!!; WW@G de novo /Z]Z@VZ@%_6_ Fusarium % F. verticillioides, F. circinatum, graminearum @ IU F. fujikuroi, F. mangiferae W G % 5664* @? ; [ et al. 56!5 et al. 56!_ V % O GS]U/%57 ; et al % 566# WW@G % % * I@ 566F2% et al.566"V RESULTS AND DISCUSSION $ $ W $ V C. austroafricana ? 77FF"!F" O V% 2% et al. 566" N F7!F 76 >46% . % % F!F5# W ]G WW@G % 4_"z/ GS]U/ % * I@ 566F2% "7_4 z 774 et al.566" V/Z]Z@VZ@ V WW@G F. dlamini, !_7E7 F. napiforme, F. nygami, F. acutatum, F. pseudoanthophilum, V C. austroafricana O F. udum, F. xylarioidesQR<et al. !""E5666 77FU Cryphonectria parasitica / F. udum, 7_"U K !! !E7 [\\ %\G 5 F. xylarioides+ !"#! F. nagamai % \G 5 $ % V Cryphonectriaceae % ? F. nagamai. The species V% [ . VOLUME 6 · NO. 1 235 \[et al. $2 I>!""F RESULTS AND DISCUSSION [ V % / Fusarium nygamai @ et al. !""4 O4!F!465" % % Fusarium nygami ___$V 76" ARTICLE O 566 4_!"65_ >46 V I+ !"E_ % 5#F!F4F % ]G 7#7FzQ 76" !4 !666666 O * I @ 566F O $ #Ez Q% ? ?+ $ % WW@G ; et al. 566# F. nygamai $ "F_#z ""z % $ % VF. nygamai is !4#E6 %QW V F. nygamai F. SEQUENCED STRAIN verticillioides 7!E U F. circinatum 77_ U F. fujikuroi 7_" U F. mangiferae 74F U et al. USA: Pennsylvania[ $ 56!_ V O % F. F. nygamai U.!4F7 $ U.5_#6 solani 4!_ U et al. 56!_ @ / / U/V.! QW F. solani ] 2 WW@G I > !""F ` !""F J.A. KlaasenIP.E. Nelson et al. 56!_ ; +*/@V UGE47F GU!5E7# 2@Z ].64!!! GUW!56" the F. nygamai ? U.#7"52.!7E;SUMF!5## % V Fusarium F. circinatum % NUCLEOTIDE SEQUENCE ACCESSION > et al. 56!7 V % NUMBER WW@G The Fusarium nygamai ? % % $ <<+`\SU+*\]+ *+>66666666V[ % WW@G Authors[ Q.S. Santana, G. Fourie, L. de Vos, !!?$?!@!\[ METHODS !!\!+!] ] <>/ !.. . $ IMA Genome-F 4D ;</ + < % @ / 54 G % ] et al566FQ . . O_666 Draft genome sequence of 546M_46 4F6MF46 % N 9 @?5666? W Leptographium lundbergii ] % @ O / $ ? G*G ] %E6 G*G / Leptographium lundbergii !"5# < ? .