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Informe De Ensayo Última versión 10 04 2018 CB SUBMAREAL ID único ILS0919_P1 Informe de Ensayo 1. Identificación Cliente Cliente CESMEC S.A. Responsable Sofia Perez Dirección Ramon Freire 50, Colina-Santiago Contacto [email protected] 2. Identificación del método de ensayo Responsable del ensayo ENVIROLAB SpA Método de ensayo Res. Exe. N°3612 Laboratorio de análisis biológicos Desarrollo ensayo Autoriza el ensayo Claudia Aburto Leiva ENVIROLAB SpA 2.1 Características del método de ensayo Previa separación de los organismos del sedimento marino, mediante el uso de un tamiz de 1 mm, posteriormente se identifican todos los organismos encontrados. La identificación se determina al nivel taxonómico más bajo posible y debe realizarse bajo lupa. Además, en el caso de las familias Capitellidae, Oweniidae, Cirratulidae y Nassaridae, es obligatorio que la determinación sea, como mínimo, a nivel de género. Descripción Posteriormente, los organismos se dejan drenar por 10 minutos para eliminar el exceso de alcohol. Luego, se pesan en una balanza analítica, por taxón, para obtener el peso húmedo alcohólico. En este informe de ensayo, la abundancia se expresa en n/m2, mientras que la biomasa va expresada en g/m2. 3. Identificación de los ítems de ensayo Tipo de muestra Sedimento marino submareal Localidad muestreo Bahía de Coronel Tipo de envase Frascos plásticos de 250 cc con contratapa Fecha muestreo 04/09//2019 Ítems de ensayo totales 30 unidades Fecha ingreso al Laboratorio 06/09/2019 Preservante Alcohol al 70% Emisión Informe de Ensayo 18/10/2019 ENVIROLAB SpA – Av. del Valle Norte 937 – Oficina – 258 – Huechuraba – Santiago 1 Última versión 10 04 2018 CB SUBMAREAL ID único ILS0919_P1 Informe de Ensayo 4. Identificación ítems de ensayo ingresados al Laboratorio Número Rótulo Número Rótulo Número Rótulo Número Rótulo 1 E1R1 11 E4R2 21 E7R3 2 E1R2 12 E4R3 22 E8R1 3 E1R3 13 E5R1 23 E8R2 4 E2R1 14 E5R2 24 E8R3 5 E2R2 15 E5R3 25 E9R1 6 E2R3 16 E6R1 26 E9R2 7 E3R1 17 E6R2 27 E9R3 8 E3R2 18 E6R3 28 E10R1 9 E3R3 19 E7R1 29 E10R2 10 E4R1 20 E7R2 30 E10R3 5. Resultados Datos obtenidos por estación de monitoreo, correspondientes a los ítems de ensayo analizados por los profesionales de ENVIROLAB SpA. ENVIROLAB SpA – Av. del Valle Norte 937 – Oficina – 258 – Huechuraba – Santiago 2 Última versión 10 04 2018 CB SUBMAREAL ID único ILS0919_P1 Informe de Ensayo Resultados Tabla 1. Resultados de abundancia y biomasa obtenidos en la Estación de Monitoreo Submareal 1. Réplica Phylum Clase Orden Familia Género Especie Abundancia (n/m2) Biomasa (g/m2) R1 Annelida Polychaeta Spionida Spionidae Rynchospio Rynchospio glutaea 3520 17,860 Littorinimorpha Calyptraeidae Crepidula - 60 394,450 Mollusca Gastropoda Neogastropoda Nassariidae Nassarius Nassarius dentifer 20 37,430 R3 Mollusca Bivalvia Mytilida Mytilidae Choromytilus Choromytilus chorus 10 503,770 Arthropoda Hexanauplia Sessilia Balanidae Balanus - 40 11,980 Tabla 2. Resultados de abundancia y biomasa obtenidos en la Estación de Monitoreo Submareal 2. Réplica Phylum Clase Orden Familia Género Especie Abundancia (n/m2) Biomasa (g/m2) - Capitellidae Capitella Capitella cf. capitata 2410 10,020 Phyllodocidae 1 - - 10 0,010 Annelida Polychaeta Phyllodocida Phyllodocidae 2 - - 10 0,010 R1 Phyllodocidae Eteone - 70 0,110 Spionida Spionidae Rynchospio Rinchospio glutaea 220 0,520 Mollusca Bivalvia Cardiida Tellinidae Macoploma - 100 0,870 ENVIROLAB SpA – Av. del Valle Norte 937 – Oficina – 258 – Huechuraba – Santiago 3 Última versión 10 04 2018 CB SUBMAREAL ID único ILS0919_P1 Informe de Ensayo Gastropoda Neogastropoda Nassariidae Nassarius Nassarius dentifer 30 44,530 Annelida Polychaeta - Capitellidae Capitella Capitella cf. capitata 160 0,700 R2 Mollusca Gastropoda Neogastropoda Nassariidae Nassarius Nassarius dentifer 10 19,210 R3 Mollusca Gastropoda Neogastropoda Nassariidae Nassarius Nassarius dentifer 10 39,540 Tabla 3. Resultados de abundancia y biomasa obtenidos en la Estación de monitoreo Submareal 3. Réplica Phylum Clase Orden Familia Género Especie Abundancia (n/m2) Biomasa (g/m2) R1 Annelida Polychaeta Phyllodocida Goniadidae Goniada - 10 0,030 R2 Mollusca Bivalvia Venerida Veneridae - - 10 1,070 Bivalvia Mytilida Mytilidae Choromytilus Choromytilus chorus 10 164,110 Mollusca R3 Gastropoda Trochida Tegulidae Tegula Tegula atra 10 20,410 Annelida Polychaeta - Orbiniidae Scoloplos - 20 0,080 Tabla 4. Resultados de abundancia y biomasa obtenidos en la Estación de monitoreo Submareal 4. Réplica Phylum Clase Orden Familia Género Especie Abundancia (n/m2) Biomasa (g/m2) R2 Mollusca Gastropoda Neogastropoda Nassariidae Nassarius Nassarius dentifer 10 19,840 ENVIROLAB SpA – Av. del Valle Norte 937 – Oficina – 258 – Huechuraba – Santiago 4 Última versión 10 04 2018 CB SUBMAREAL ID único ILS0919_P1 Informe de Ensayo Tabla 5. Resultados de abundancia y biomasa obtenidos en la Estación de monitoreo Submareal 5. Réplica Phylum Clase Orden Familia Género Especie Abundancia (n/m2) Biomasa (g/m2) Fartulum- - 5740 8,220 Mollusca Gastropoda Littorinimorpha Caecidae Caecum Caecum chilense 3040 1,980 Arthropoda Pycnogonida - - - - 50 0,200 Echinodermata Ophiuroidea - - - - 10 0,950 R1 Malacostraca Amphipoda - - - 20 0,270 Arthropoda Ostracoda 1 - - - - 30 0,260 Ostracoda 2 - - - - 60 0,060 Bivalvia Venerida Veneridae Venus Venus antiqua 70 1,170 Mollusca Bivalvia - - - - 30 0,070 Fartulum - 6330 5,110 Mollusca Gastropoda Littorinimorpha Caecidae Caecum Caecum chilense 2030 1,360 Ostracoda 1 - - - - 50 0,270 R2 Arthropoda Ostracoda 2 - - - - 130 0,280 Mollusca Bivalvia Venerida Veneridae Venus Venus antiqua 20 1,590 Sipuncula - - - - - 10 0,150 ENVIROLAB SpA – Av. del Valle Norte 937 – Oficina – 258 – Huechuraba – Santiago 5 Última versión 10 04 2018 CB SUBMAREAL ID único ILS0919_P1 Informe de Ensayo Gastropoda Neogastropoda Nassariidae Nassarius Nassarius gayii 10 1,910 Mollusca Bivalvia Venerida Mactridae Mulinia Mulinia edulis 170 0,830 Tabla 6. Resultados de abundancia y biomasa obtenidos en la Estación de monitoreo Submareal 6. Réplica Phylum Clase Orden Familia Género Especie Abundancia (n/m2) Biomasa (g/m2) Mollusca Bivalvia Mytilida Mytilidae Semimytilus Semimytilus algosus 10 0,370 R1 Annelida Polychaeta - Capitellidae Capitella Capitella cf. capitata 30 0,150 Tabla 7. Resultados de abundancia y biomasa obtenidos en la Estación de monitoreo Submareal 7. Réplica Phylum Clase Orden Familia Género Especie Abundancia (n/m2) Biomasa (g/m2) Sipuncula - - - - - 60 0,500 Spionida Spionidae Rynchospio Rhynchospio glutaea 150 0,900 R3 Hesionidae - - 10 0,010 Annelida Polychaeta Phyllodocida Phyllodocidae Eteone - 10 0,070 Glyceridae Hemipodia - 10 0,200 ENVIROLAB SpA – Av. del Valle Norte 937 – Oficina – 258 – Huechuraba – Santiago 6 Última versión 10 04 2018 CB SUBMAREAL ID único ILS0919_P1 Informe de Ensayo Tabla 8. Resultados de abundancia y biomasa obtenidos en la Estación de monitoreo Submareal 8. Réplica Phylum Clase Orden Familia Género Especie Abundancia (n/m2) Biomasa (g/m2) R1 Mollusca Gastropoda Neogastropoda Nassariidae Nassarius Nassarius dentifer 10 16,790 Mollusca Gastropoda Neogastropoda Nassariidae Nassarius Nassarius dentifer 30 44,830 R2 Phyllodocida Nereididae Nereis Nereis callaona 10 0,380 Annelida Polychaeta Eunicida Onuphidae Diopatra Diopatra chiliensis 10 3,910 Mollusca Gastropoda Neogastropoda Nassariidae Nassarius Nassarius dentifer 100 181,900 R3 Annelida Polychaeta Eunicida Lumbrineridae Scoletoma - 10 0,070 Tabla 9. Resultados de abundancia y biomasa obtenidos en la Estación de monitoreo Submareal 9. Réplica Phylum Clase Orden Familia Género Especie Abundancia (n/m2) Biomasa (g/m2) Annelida Polychaeta - Capitellidae Capitella Capitella cf. capitata 310 2,570 R1 Mollusca Bivalvia Venerida Mactridae Mulinia Mulinia edulis 10 67,790 R2 Annelida Polychaeta - Capitellidae Capitella Capitella cf. capitata 320 2,070 R3 Annelida Polychaeta - Capitellidae Capitella Capitella cf. capitata 230 0,930 ENVIROLAB SpA – Av. del Valle Norte 937 – Oficina – 258 – Huechuraba – Santiago 7 Última versión 10 04 2018 CB SUBMAREAL ID único ILS0919_P1 Informe de Ensayo Tabla 10. Resultados de abundancia y biomasa obtenidos en la Estación de monitoreo Submareal 10. Réplica Phylum Clase Orden Familia Género Especie Abundancia (n/m2) Biomasa (g/m2) Mollusca Bivalvia Cardiida Tellinidae Macoploma - 70 0,150 R1 Sipuncula - - - - - 10 0,010 Annelida Polychaeta Phyllodocida Nephtyidae Nephtys - 10 0,200 R2 Annelida Polychaeta Phyllodocida Goniadidae Goniada Goniada uncinigera 20 0,260 Mollusca Bivalvia Cardiida Tellinidae Macoploma - 30 0,050 Sipuncula - - - - - 10 0,220 R3 Goniadidae Goniada Goniada uncinigera 10 0,160 Annelida Polychaeta Phyllodocida Phyllodocidae Eteone - 10 0,150 Arthropoda Malacostraca Cumacea - - - 30 0,050 ENVIROLAB SpA – Av. del Valle Norte 937 – Oficina – 258 – Huechuraba – Santiago 8 Última versión 10 04 2018 CB SUBMAREAL ID único ILS0919_P1 Informe de Ensayo 6. Observaciones Condiciones de los ítems de ensayo, además de las observaciones propias del proceso de ensayo realizado por los profesionales de ENVIROLAB SpA. Indicar si hay alguna modificación al método de análisis. 1. Estaciones de monitoreo submareal E1R2, E4R1, E4R3, E5R3, E6R2, E6R3, E7R1 y E7R2 resultaron ser NEGATIVAS. 7. Declaración ENVIROLAB SpA: Tratamiento de ítems de ensayo El muestreo asociado a la extracción de muestras fue realizado por profesionales de ENVIROLAB SpA y está sujeto a la Resolución Exenta Nº3612 numeral 28, sin modificaciones sustanciales. Se adjunta a este documento el informe de muestreo IMS0919_P1. Los resultados expuestos en el informe de ensayo ILS0919_P1 son de correspondencia fidedigna a los ítems de ensayo ingresados a las dependencias de ENVIROLAB SpA (n=30), son válidos solo para estas muestras y no presentan información adicional a la observada por los profesionales de ENVIROLAB SpA durante el proceso de análisis. Claudia Aburto Leiva Jefe Laboratorio ENVIROLAB SpA ENVIROLAB SpA – Av. del Valle Norte 937 – Oficina – 258 – Huechuraba – Santiago 9 .
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