Comparison of Pharmaceutical, Illicit Drug, Alcohol, Nicotine and Caffeine
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Baz-Lomba et al. BMC Public Health (2016) 16:1035 DOI 10.1186/s12889-016-3686-5 RESEARCH ARTICLE Open Access Comparison of pharmaceutical, illicit drug, alcohol, nicotine and caffeine levels in wastewater with sale, seizure and consumption data for 8 European cities Jose Antonio Baz-Lomba1,2*, Stefania Salvatore2, Emma Gracia-Lor3, Richard Bade4, Sara Castiglioni3, Erika Castrignanò5, Ana Causanilles6, Felix Hernandez4, Barbara Kasprzyk-Hordern5, Juliet Kinyua7, Ann-Kathrin McCall8, Alexander van Nuijs7, Christoph Ort8, Benedek G. Plósz9, Pedram Ramin9, Malcolm Reid1, Nikolaos I. Rousis3, Yeonsuk Ryu1, Pim de Voogt6, Jorgen Bramness2 and Kevin Thomas1 Abstract Background: Monitoring the scale of pharmaceuticals, illicit and licit drugs consumption is important to assess the needs of law enforcement and public health, and provides more information about the different trends within different countries. Community drug use patterns are usually described by national surveys, sales and seizure data. Wastewater-based epidemiology (WBE) has been shown to be a reliable approach complementing such surveys. Method: This study aims to compare and correlate the consumption estimates of pharmaceuticals, illicit drugs, alcohol, nicotine and caffeine from wastewater analysis and other sources of information. Wastewater samples were collected in 2015 from 8 different European cities over a one week period, representing a population of approximately 5 million people. Published pharmaceutical sale, illicit drug seizure and alcohol, tobacco and caffeine use data were used for the comparison. Results: High agreement was found between wastewater and other data sources for pharmaceuticals and cocaine, whereas amphetamines, alcohol and caffeine showed a moderate correlation. methamphetamine and 3,4- methylenedioxymethamphetamine (MDMA) and nicotine did not correlate with other sources of data. Most of the poor correlations were explained as part of the uncertainties related with the use estimates and were improved with other complementary sources of data. Conclusions: This work confirms the promising future of WBE as a complementary approach to obtain a more accurate picture of substance use situation within different communities. Our findings suggest further improvements to reduce the uncertainties associated with both sources of information in order to make the data more comparable. Keywords: Wastewater-based epidemiology, Drug consumption, Correlation, Europe-wide study, Seizures, Sales statistics * Correspondence: [email protected] 1Norwegian Institute for Water Research (NIVA), Gaustadalléen 21, Oslo NO-0349, Norway 2Norwegian Centre for Addiction Research, Faculty of Medicine, University of Oslo, PO box 1078, Blindern, Oslo 0316, Norway Full list of author information is available at the end of the article © 2016 The Author(s). Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. Baz-Lomba et al. BMC Public Health (2016) 16:1035 Page 2 of 11 Background been used to determine nicotine use through the measure- The chemical analysis of the exogenous biomarkers of ment of its residues in wastewater at national level [6, 7, 28]. drug consumption in wastewater has been shown to be an These data were compared with local tobacco sales statistics interesting approach for studying drug use within a de- finding good agreement [29]. Caffeine is present in coffee fined population [1]. The approach, termed wastewater- and tea as well as other products such as energy drinks, based epidemiology (WBE), was first applied in 2008 to chocolate and certain medicines. Caffeine occurrence has study illicit drug use in three Italian cities [2] and it has been investigated to assess its patterns of use across different since been extended to a wider range of exogenous bio- communities [7, 30]. markers specific to the use of alcohol [3–5], tobacco [6], Very few studies to date have correlated wastewater caffeine [7] and selected pharmaceuticals [8, 9]. To date data with other sources of data. So far, pharmaceutical there has been very limited focus on evaluating the rela- concentrations in wastewater have been related to the tionship between WBE data to other drug use measures. amounts used in a population by using prescription data The use of pharmaceuticals in society is usually deter- [11, 31–33]. A Spanish study compared wastewater con- mined by prescription data or self-reports. However, centrations of 17 pharmaceuticals with annual sales data pharmaceutical prescription or sales data involve several and found a good correlation [31]. The same was true sources of uncertainties such as the inappropriate use of for studies from Australia [32], Italy [33], and Belgium a medication, household disposal through the toilet or [11]. One study compared cocaine loads in wastewater sink, sales without prescription or illegal acquisition in Oslo with cocaine measures from population survey [10–12]. Self-reports on adherence are potentially sub- data and drug tests among car drivers and found a good ject to recall and reporter bias [13]. An alternative could correlation [21]. A recent study in Belgium compared be to quantitatively measure pharmaceuticals in waste- WBE results with survey data collected simultaneously water as an indicator of use [14–16]. in a small community of around 30,000 inhabitants for Europe has a significant market for illicit drugs sus- alcohol and nicotine metabolites [34]. No significant tained by both domestic production and import from correlations were found, but the study was impaired by a other regions [17]. Different monitoring approaches have typically low response to the surveys. Discrepancies been used to investigate the scale of drug use. Police and between wastewater and national survey data have also custom’s seizures, population surveys, hospital admissions, been found in Australia [35]. drug-overdose deaths or treatment programs have been The present study aims to compare WBE data with the most reliable data for drug use estimates [18–20]. other sources of drug use data in order to assess its correl- Under-reporting is still apparent despite the use of a num- ation. The study uses concentrations of pharmaceuticals, ber of different sources of information to improve the illicit drugs, alcohol, nicotine and caffeine in wastewater level of accuracy of self-reporting [21]. Seizure data may samples from different European cities and compares not give the correct picture and surveys suffer from these with other sources of data for drug use. The data reporting biases and low response rates [22]. WBE has used for comparison were pharmaceutical prescriptions been introduced as a promising alternative or addition to (morphine, methadone, atenolol, metoprolol, methylphen- these sources of data [23–25]. idate, carbamazepine, citalopram, paracetamol, oxazepam There is also a need to be able to accurately determine and diclofenac), police drug seizure data for illicit drugs the consumption of licit drugs, such as alcohol, nicotine (cocaine, amphetamine, and MDMA (ecstasy)), and con- and caffeine. Alcohol consumption can be estimated from sumption data for alcohol, tobacco and caffeine metabo- sales statistics but trafficking, illegal production, stock pil- lites (EtS; cotinine, nicotine and hydroxycotinine; caffeine, ing or waste can affect such data. Reid and co-workers for paraxanthine, 1-methylxanthine, 7-methylxanthine, 1- the first time applied WBE to obtain complementary methyluric acid and 1,7-dimethyluric acid). information on community alcohol consumption by ana- lysing the alcohol metabolite, ethyl sulfate (EtS) in waste- Methods water [4]. This approach has since been used in other Wastewater samples studies to report the rates of alcohol consumption in pop- Twenty-four hour composite inlet sewage samples were ulations [3, 26], but the value of these biomarkers has yet collected from 8 different European wastewater treatment to be determined. Tobacco use is commonly deduced plants (WWTP) over 7 consecutive days in March 2015. from tobacco sales statistics. Prevalence surveys and the All samples were stored in plastic containers, immediately aggregate between production and trade statistics can also frozen at −20 °C to prevent degradation of the urinary me- yield the total consumption estimates [27]. However, these tabolites and sent to Oslo and Milan within 24 h in cool data may bias the total consumption estimates (similar to boxes with dry ice or ice packs to keep the samples frozen. alcohol) indicating that additional methods are required in The average wastewater flow rate (litres per day (L/day)) order to improve tobacco use estimations. WBE has also through each of the treatment plants was recorded for Baz-Lomba et al. BMC Public Health (2016) 16:1035 Page 3 of 11 each of the daily samples. Samples were collected every methanol-ultrapure water (20:80, v/v), centrifuged and morning between 07:00 and 8:30 except for Zurich where transferred into glass vials for instrumental analysis. Fi- it was performed