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AC Griffonia Lysate Advanced 16634 AC Griffonia Lysate Advanced Immediately Perceivable Results + Neuro-Cosmetics + Lifting AC Griffonia Lysate Advanced 16634 AC Griffonia Lysate Advanced Immediately Perceivable Results + Neuro-Cosmetics + Lifting Product Code: 16634 INCI Name: Griffonia Simplicifolia Seed Extract INCI Status: Conforms Suggested Use Levels: 1.0 – 10.0% Suggested Applications: Antioxidant, Lifting, Tightening, UV Protection ISO compliant NI & NOI In - In 16128 v - egan - vitro Solubility: Water Soluble vivo 16634 AC Griffonia Lysate Advanced Meeting Market Demand and Expectations • Formulator’s best tools are ingredients that provide multiple benefits • Enhances the product’s marketing appeal • Minimizes inventory and production time • Consumers also prefer products that provide multiple benefits • AC Griffonia Lysate Advanced is a natural and innovative ingredient • Coincides with trends for cosmetics that provide multiple and immediate benefits • Perceivable skin tightening • Protects against UV induced damage • Minimizes the appearance of fine lines and wrinkles • Standardized for 1.0% 5-Hydroxy Tryptophan (HTP) 16634 AC Griffonia Lysate Advanced 5-Hydroxytryptophan (5-HTP): a Precursor to Serotonin • The feelings that accompany the use of cosmetics push neuro-cosmetics to the forefront of modern skin care. • Serotonin, a neurotransmitter known to promote feelings of happiness, can be capitalized on and marketed as such, to seamlessly enhance products specifically designed as neuro-cosmetics. • The naturally occurring amino acid, 5-hydroxytryptophan (5-HTP), is the chemical precursor in the biosynthesis of serotonin. When used, 5-HTP transforms personal care formulas into true neuro-cosmetics, capable of making consumers feel and look good. • AC Griffonia Lysate Advanced takes advantage of the 5-HTP content in griffonia seeds, providing formulators with a neuro-cosmetic material that delivers immediately perceivable results and long-term benefits for anti-aging skin care. 16634 AC Griffonia Lysate Advanced Griffonia simplicifolia • Griffonia simplicifolia is an African shrub and a natural source of the compound 5-HTP. • Since the plant is constantly under intense UV exposure it needed to evolve a means to protect itself from UV damage. • Research has shown that 5-HTP content in the plant is integral to its survival. 16634 AC Griffonia Lysate Advanced Neuro-Cosmetic Polysaccharide Complex • Active Concepts has extracted the high molecular weight carbohydrates from this African shrub during the manufacturing process for additional anti-aging benefits. • High molecular weight carbohydrates act as strong film-formers and are responsible for perceivable sensorial benefits associated with AC Griffonia Lysate Advanced. • The combination of components that make up AC Griffonia Lysate Advanced result in a long-term anti-aging product which also imparts a tangible and supple film on the skin. • This film not only protects against environmental damage, but also immediately tightens and lifts the skin, revealing a firm, younger-looking complexion. 16634 AC Griffonia Lysate Advanced 5-HTP to Prevent UV Damage Inhibition of Apoptosis 70 • Lysek et al. reported 5-HTP is capable of suppressing UV induced apoptosis in human monocytes. 60 • The decrease in apoptosis is due to the decrease in 50 UV induced damage, which triggers the cell death. 40 • Clearly, these findings suggest that using 5-HTP may be a novel approach to inhibiting extrinsic damage 30 caused by UV radiation. Degree of Apoptosis (%) Apoptosis of Degree 20 • As demonstrated in Figure 1, an increase in the concentration of 5-HTP shows a decrease in UV 10 induced apoptosis. 0 • By inhibiting UV induced damage, 5-HTP may No UV, No UV, 10 UV, 10 mM 5- UV, 5.0 mM UV, 1.0mM 5- UV, 0.5 mM UV, 0.0mM 5- UV, 5.0mM ameliorate anticipated skin damage caused by over 0.0mM 5-HTP mM 5-HTP HTP 5-HTP HTP 5-HTP HTP ACC exposure. Figure 1. Apoptosis [%] of UV-exposed (λ = 254 nm,1 mW· cm-2, 2 h) human monocytes in the presence of different concentrations of 5-HTP . ACC = N-acetylcysteine, a known anti-apoptic agent.1 1. Lysek, Nicola et al. L-5Hydroxytryptophan: Antioxidant and Anti-Apoptotic Principle of the Intertidal Sponge Hymeniacidon Heliophila. A Naturforsch; 58c:568-572 (2003). 16634 AC Griffonia Lysate Advanced Antioxidant ORAC Assay ORAC Assay Protocol 70 60 • Solutions of AC Griffonia Lysate Advanced and Trolox® were prepared in 10% DMSO, 75nM 50 potassium phosphate buffer • Materials were prepared at three dilutions 40 • For the ORAC assay – test material and Trolox® 30 combined with fluorescein and incubated 20 • Fluorescent measurements were taken every 2 Antioxidant Capacity(µMTE) minutes for 2 hours 10 • ORAC values of samples calculated 0 • AC Griffonia Lysate Advanced is capable of 50µM Trolox® 6.25µM Trolox® 0.5% AC Griffonia 0.1% AC Griffonia 0.05% AC Griffonia Lysate Advanced Lysate Advanced Lysate Advanced providing antioxidant benefits Figure 2. Antioxidant capacities. 16634 AC Griffonia Lysate Advanced in vivo Analysis - Immediate Skin Lifting Benefits Protocol Ratings on Scale of 1-10 (Feeling of Tightness) • 6 M/F Participants over 3 minute time period Active Placebo • 5.0% AC Griffonia Lysate Advanced in base serum Participant 1 8 2 was assessed for its ability to provide immediately Participant 2 6 5 perceivable skin tightening benefits compared to Participant 3 5 7 the base serum alone. Participant 4 6 7 • According to the results of the assessment, 66.7% of participants were able to perceive a significant Participant 5 6 4 tightening effect on the area around the eye Participant 6 6 1 where the experimental (5.0% AC Griffonia Lysate Advanced in base serum) was applied. Average 6.2 4.3 • Additionally, the experimental (5.0% AC Griffonia Lysate Advanced in base serum) delivered 36.2% Figure 3. Participant assessment of tightness. articipants were asked to rate the feeling of tightness on a scale of 1- 10 for each under eye area, where 1 represents no perceived improvement. This data provides an more of a perceivable tightening effect than the assessment of perceivable skin tightening. base serum alone. 16634 AC Griffonia Lysate Advanced in vivo Analysis - Immediate Skin Lifting Benefits Protocol • Furthermore, photos were used to analyze effect on the visible wrinkle feature count. • Baseline photos were taken prior to application of the test materials. Photos were taken again three minutes following application of the test materials. • Photographic assessments were performed using the VISIA Complexion Analysis System • AC Griffonia Lysate Advanced can be used to visibly reduce the appearance of fine lines and wrinkles while providing a noticeable tightening effect on skin. Figure 4. Participant #2 treated with 5.0% AC Griffonia Lysate Advanced in Base Serum displays a reduction (10.0%) • For this reason, this exceptional ingredient is ideal in wrinkle feature counts from beginning of treatment (T=0) to T=3 minutes via VISIA Image Analysis. Images on the top are participant #2 with image enhancement, through VISIA, which provides higher to add to cosmetic and personal care applications visualization of feature changes. Images on the bottom are natural photos of participant #2. where immediate lifting benefits are desired. 16634 AC Griffonia Lysate Advanced in vivo Analysis - Immediate Skin Lifting Benefits Protocol • Furthermore, photos were used to analyze effect on the visible wrinkle feature count. • Baseline photos were taken prior to application of the test materials. Photos were taken again three minutes following application of the test materials. • Photographic assessments were performed using the VISIA Complexion Analysis System • AC Griffonia Lysate Advanced can be used to visibly reduce the appearance of fine lines and wrinkles while providing a noticeable tightening effect on skin. Figure 5. Participant #5 treated with 5.0% AC Griffonia Lysate Advanced in Base Serum displays a reduction (19.0%) • For this reason, this exceptional ingredient is ideal in wrinkle feature counts from beginning of treatment (T=0) to T=3 minutes via VISIA Image Analysis. Images on the top are participant #5 with image enhancement, through VISIA, which provides higher to add to cosmetic and personal care applications visualization of feature changes. Images on the bottom are natural photos of participant #5. where immediate lifting benefits are desired. 16634 AC Griffonia Lysate Advanced Immediately Perceivable Results + Neuro-Cosmetics + Lifting Product Code: 16634 INCI Name: Griffonia Simplicifolia Seed Extract INCI Status: Conforms Suggested Use Levels: 1.0 – 10.0% Suggested Applications: Antioxidant, Lifting, Tightening, UV Protection ISO compliant NI & NOI In - In 16128 v - egan - vitro Solubility: Water Soluble vivo Active Concepts, LLC Lincolnton, NC. USA www. activeconceptsllc.com Office: +1 704 276 7100 AC Griffonia Lysate Advanced [email protected].
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