Inaugural ASAS–CAAV Asia Pacif ic Rim Conference November 8–10, 2009 Abstracts http://www.asas.org/pacif icrim09/ Abstracts of papers presented at the Inaugural ASAS-CAAV Asia Pacifi c Rim Meeting November 8–10, 2009 Beijing, China 2 Scientifi c Program Table of Contents Sunday, November 8 SYMPOSIA AND ORAL SESSIONS Animal Health, Growth, Physiology, Endocrinology. 5 Biosecurity and Food/Feed Security . 7 SYMPOSIUM: EAAP-ASAS Genetic Symposium: Importance of Genotype by Environment Interaction in Animal Breeding. 9 Nonruminant Nutrition I . 11 OTHER EVENTS Opening Ceremony . 15 Monday, November 9 SYMPOSIA AND ORAL SESSIONS Small Ruminant, Forages and Pastures . 16 Advances in Digestive Physiology Metabolism and Health . 17 Nonruminant Nutrition II . 18 Producing Muscle that Tastes Good with a High Nutritive Value . 22 SYMPOSIUM: Lactation Symposium . 23 Nonruminant Nutrition III . 25 Ruminant Nutrition . 28 POSTER PRESENTATIONS Dairy Nutrition . 32 Nonruminant Nutrition . 32 Poultry Metabolism and Nutrition . 42 Ruminant Nutrition . 46 Tuesday, November 10 SYMPOSIA AND ORAL SESSIONS SYMPOSIUM: Meat Safety . 53 Ruminant Nutrition, Growth, and Development . 55 Swine Production . 55 Environmental Impacts of Cattle, Swine and Poultry Production . 57 SYMPOSIUM: Publishing in JAS . 58 International Partnerships and Student Exchanges . 58 POSTER PRESENTATIONS Animal Behavior and Well-Being. 60 Animal Health. 61 Beef Species. 65 Breeding and Genetics . 66 Companion Animals . 70 Food Safety . 70 Forages and Pastures . 73 3 Growth and Development. 76 International Animal Agriculture . 77 Lactation Biology . 79 Physiology and Endocrinology . 81 Poultry Environment and Management. 83 Poultry Genetics. 84 Poultry Immunology . 84 Poultry Physiology, Endocrinology, and Reproduction. 85 Production, Management and the Environment . 86 Small Ruminants . 88 Swine Species . 91 Subject Index . 93 Author Index . 97 4 Sunday, November 8, 2009 SYMPOSIA AND ORAL SESSIONS Animal Health, Growth, Physiology, Endocrinology 1 Advanced needle-free injection technology. W. Shao*1, 3 Lipoic acid attenuates the anaphylactic reactions induced C. Funk2, and J. Poiron2, 1Sino Waypoint Consulting, Inc., Ottawa, Ontario, by soybean β-conglycinin in a rat model. P. F. Han*, X. Ma, and J. D. Yin, Canada, 2AcuShot, Inc., Winnipeg, Manitoba, Canada. State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing, China. AcuShot patented technology is the next generation in needle-free liquid injection devices specifi cally designed for agricultural use. This technology The purpose of this study was to evaluate the effects of feeding a low dose of provides the veterinarian and animal health care industries with revolutionary lipoic acid on attenuating soybean β-conglycinin-induced hypersensitivity by needle-free device choices that enable spot treatment of single animals and using a rat model, with ovalbumin as the positive allergic control. Forty-eight mass vaccination of large herds of animals in a cost-effective, effi cient, safe, recently weaned, male, Sprague-Dawley rats were assigned to 1 of 4 treatments and easy-to-use format. AcuShot technology has been adopted in the AcuShot and fed a cornstarch- and casein-based diet either unsupplemented (groups I, needle-free injection device for delivering liquid medication under pressure II, and III) or supplemented with 25 mg/kg of lipoic acid (group IV). Rats in into an animal. The AcuShot needle-free injection device is a battery-operated, groups III and IV were sensitized with 20 mg of β-conglycinin on d 1, 10, 17, high-workload, handheld, mass-vaccination injector. It can be used on a and 24 by means of intragastric gavage, whereas rats in group II were sensitized hands-free stand for smaller livestock, such as newborn piglets and poultry, with 20 mg of ovalbumin. The control group (group I) was gavaged with casein or can be used with a remote injection handpiece for easy use with larger by using the same treatment schedule. On d 31, rats received a double dose of livestock, such as the most seasoned sow or cow. The injector can administer β-conglycinin, ovalbumin, or casein, respectively. Blood was obtained from the any vaccine or medication intramuscularly, subcutaneously, or intradermally tail vein of each rat 3 h after intragastric gavage. On d 32, all rats were slaughtered (transdermally). AcuShot needle-free injection devices come in two models: by cervical dislocation. The spleen and small intestine were removed, and then 1) The AcuShot S provides extremely accurate doses at micro levels and can the intestinal tissues were stored in liquid nitrogen until analysis. Untreated, deliver traditional vaccines or supplements in doses ranging from 0.05 mL up β-conglycinin-sensitized rats (group III) demonstrated an increase in serum IgE to 1.35 mL in increments of 0.05 mL; and 2) the AcuShot A provides extremely and histamine release, but had reduced growth performance and poorer feed accurate doses at levels ranging from 0.2 to 2.5 mL in increments of 0.1 mL. conversion compared with the control rats (P < 0.05; group I), similar to the Both the AcuShot S and AcuShot A are electronically controlled and have ovalbumin-sensitized rats (group II). A low dose of lipoic acid signifi cantly onboard electronics that monitor key components and injection characteristics (P < 0.05) improved BW gain and feed conversion while reducing serum for each shot and that provide accurate dosage selection and delivery. AcuShot IgE and histamine release. Moreover, our research indicated that lipoic acid injectors are easily adapted to any supply container, regardless of size. The supplementation increased interferon-γ but decreased IL-4 (P < 0.05), which AcuShot needle-free injection device is able to perform thousands of injections means that the Th1-type immune response was increased to prevent soybean per battery charge, eliminating downtime and increasing effi ciency. It is safer allergies. Taken together, a low dose of lipoic acid has the potential to be used for livestock, with a reduced chance of infection or disease transmission, and as an immunomodulator to prevent soybean β-conglycinin-induced allergies by eliminates the ongoing cost and disposal of hazardous needles. It has been used amending the balance of cytokines. in Canada, the United States, Mexico, South America, Japan, Korea, and many Key Words: α-lipoic acid, β-conglycinin, anaphylaxis countries in the European Union. It has recently been introduced in China. Key Words: needle free, injection, vaccination 4 Discrepancies between in vitro and in vivo afl atoxin binding. J. N. Broomhead* and F. Chi, Amlan International, Chicago, IL, USA. 2 Isolation of mink enteritis virus and application of immune An in vitro binding study and an in vivo chicken study were conducted to yolk antibody. T. Tingting* and Z. Yanlong, Northeast Forest University, test the effi cacy and safety of organically modifi ed clays (OMC) in binding Harbin, China. afl atoxin B1 (AFL). In vitro mycotoxin binding was conducted at physiological conditions of the stomach (pH 3.0), followed by physiological conditions the Mink enteritis virus (MEV) infection is a highly contagious disease with a intestine (pH 6.5) at 50:1 binder-to-toxin ratio. The chick study consisted of mortality of up to 90%. Since this disease was fi rst recognized in Canada in 250 one-day-old male broiler chicks assigned to 10 treatments, with 5 replicate 1949 by Schofi eld, it has been prevalent all over the world, mainly because the pens of 5 chicks each. Four OMC were fed either alone (0.5% dietary inclusion) virus can survive in harsh environmental conditions for a long time and evolve or in combination with 2 ppm of AFL. Chicks were placed in battery brooders by natural mutation. Mink enteritis is characterized by severe mucoenteritis. and fed the experimental diets for 21 d. On d 21, three birds per replicate were This paper deals with a case of MEV isolated in Jilin Province in 2007; our euthanized with CO and weighed, and blood was drawn from 2 birds per pen purpose was examine whether the immunoglobulin derived from chicken egg 2 for serum chemistry analysis; livers were removed from 3 birds per pen and yolk (IgY) against infection by MEV could have any protective effect in mink. weighed for determination of relative liver weight (RLW). In vitro AFL binding Mink enteritis virus samples were collected from infected mink intestines based results were highly variable: OMC A, B, C, and D bound 44, 49, 63, and 81% on clinical symptoms. The embryonic feline kidney cell line F81 was used to AFL, respectively. Feeding AFL alone reduced (P < 0.05) BW gain (BWG), enrich the viruses. The viruses were then identifi ed by plaque assay and cultured feed intake, and serum protein and increased RLW (P < 0.05). Contrary to the in in a 96-well plate. Finally, we isolated the virus, which could agglutinate red vitro results, OMC A and B improved (P < 0.05) BWG and OMC A improved (P blood cells of the pig, but not the human, chicken, or rabbit. This was confi rmed < 0.05) RLW when added to the 2 ppm of AFL diet. No improvement in serum by PCR amplifi cation by a special primer of MEV. Moreover, the isolated virus protein (P < 0.05) was observed with the inclusion of any OMC to the AFL diet. could infect mink. To prepare IgY samples, we vaccinated 14-wk-old laying A signifi cant reduction (P < 0.05) in BWG was observed when feeding OMC C hens with MEV samples and then extracted egg yolk antibodies with chloroform alone, and an increase (P < 0.05) in serum aspartate aminotransferase was seen and DEAE. The hemagglutination-inhibition test, the agar spread method, and when feeding OMC B or C alone, suggesting possible toxicity when feeding indirect ELISA were used to identify the IgY. The anti-MEV IgY were then these OMC.