ARPC1A Antibody Cat. No.: 42-980
ARPC1A Antibody
42-980 (0.1ug/ml) staining of fetal Mouse Brain (A) and adult Rat Brain (B) lysates (35ug protein 42-980 (5ug/ml) staining of paraffin embedded Human Heart. in RIPA buffer). Primary incubation was 1 hour. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. Detected by chemiluminescence.
Specifications
HOST SPECIES: Goat
SPECIES REACTIVITY: Human, Mouse, Rat
HOMOLOGY: Expected Species Reactivity based on sequence homology: Pig, Cow
IMMUNOGEN: The immunogen for this antibody is: C-DFKTLESSIQGLR
TESTED APPLICATIONS: ELISA, IHC, WB
September 26, 2021 1 https://www.prosci-inc.com/arpc1a-antibody-42-980.html Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 45kDa band observed in lysates of cell line HeLa (calculated MW of 41.6kDa according to NP_006400.2). Approx 37kDa band observed in fetal Mouse Brain and adult Rat Brain APPLICATIONS: lysates (calculated MW of 41.6kDa according to Mouse NP_062741.1). Recommended concentration: 0.1-0.3ug/ml.Immunohistochemistry:Paraffin embedded Human Heart. Recommended concentration: 5ug/ml.
This antibody is expected to recognize both reported isoforms (NP_006400.2; SPECIFICITY: NP_001177925.1). No cross-reactiviry expected with ARPC1B.
PREDICTED MOLECULAR Approx 45 kDa WEIGHT:
Properties
Purified from goat serum by ammonium sulphate precipitation followed by antigen PURIFICATION: affinity chromatography using the immunizing peptide.
CLONALITY: Polyclonal
CONJUGATE: Unconjugated
PHYSICAL STATE: Liquid
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum BUFFER: albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
CONCENTRATION: 500 ug/mL
STORAGE CONDITIONS: Aliquot and store at -20˚C. Minimize freezing and thawing.
Additional Info
OFFICIAL SYMBOL: ARPC1A
ARPC1A, actin related protein 2/3 complex, subunit 1A, 41kDa, Arc40, SOP2Hs, SOP2L, ALTERNATE NAMES: OTTHUMP00000206034, SOP2-like protein, actin binding protein (Schizosaccharomyces pombe sop2-like), actin-related protein 2/3 complex subunit 1A
ACCESSION NO.: NP_006400.2, NP_001177925.1
PROTEIN GI NO.: 22907052
GENE ID: 10552
ANTIBODIES FOR RESEARCH USE ONLY.
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September 26, 2021 2 https://www.prosci-inc.com/arpc1a-antibody-42-980.html