ARPC1A Antibody Cat. No.: 42-980 ARPC1A Antibody 42-980 (0.1ug/ml) staining of fetal Mouse Brain (A) and adult Rat Brain (B) lysates (35ug protein 42-980 (5ug/ml) staining of paraffin embedded Human Heart. in RIPA buffer). Primary incubation was 1 hour. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. Detected by chemiluminescence. Specifications HOST SPECIES: Goat SPECIES REACTIVITY: Human, Mouse, Rat HOMOLOGY: Expected Species Reactivity based on sequence homology: Pig, Cow IMMUNOGEN: The immunogen for this antibody is: C-DFKTLESSIQGLR TESTED APPLICATIONS: ELISA, IHC, WB September 26, 2021 1 https://www.prosci-inc.com/arpc1a-antibody-42-980.html Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 45kDa band observed in lysates of cell line HeLa (calculated MW of 41.6kDa according to NP_006400.2). Approx 37kDa band observed in fetal Mouse Brain and adult Rat Brain APPLICATIONS: lysates (calculated MW of 41.6kDa according to Mouse NP_062741.1). Recommended concentration: 0.1-0.3ug/ml.Immunohistochemistry:Paraffin embedded Human Heart. Recommended concentration: 5ug/ml. This antibody is expected to recognize both reported isoforms (NP_006400.2; SPECIFICITY: NP_001177925.1). No cross-reactiviry expected with ARPC1B. PREDICTED MOLECULAR Approx 45 kDa WEIGHT: Properties Purified from goat serum by ammonium sulphate precipitation followed by antigen PURIFICATION: affinity chromatography using the immunizing peptide. CLONALITY: Polyclonal CONJUGATE: Unconjugated PHYSICAL STATE: Liquid Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum BUFFER: albumin. Aliquot and store at -20°C. Minimize freezing and thawing. CONCENTRATION: 500 ug/mL STORAGE CONDITIONS: Aliquot and store at -20˚C. Minimize freezing and thawing. Additional Info OFFICIAL SYMBOL: ARPC1A ARPC1A, actin related protein 2/3 complex, subunit 1A, 41kDa, Arc40, SOP2Hs, SOP2L, ALTERNATE NAMES: OTTHUMP00000206034, SOP2-like protein, actin binding protein (Schizosaccharomyces pombe sop2-like), actin-related protein 2/3 complex subunit 1A ACCESSION NO.: NP_006400.2, NP_001177925.1 PROTEIN GI NO.: 22907052 GENE ID: 10552 ANTIBODIES FOR RESEARCH USE ONLY. For additional information, visit ProSci's Terms & Conditions Page. September 26, 2021 2 https://www.prosci-inc.com/arpc1a-antibody-42-980.html.