Structure and Coding Properties of Bsl, a Maize Retrovirus-Like Transposon
Total Page:16
File Type:pdf, Size:1020Kb

Load more
Recommended publications
-
The Primer Binding Site on the RNA Genome of Human and Simian Immunodeficiency Viruses Is Flanked by an Upstream Hairpin Structure Benjamin Berkhout
1997 Oxford University Press Nucleic Acids Research, 1997, Vol. 25, No. 20 4013–4017 The primer binding site on the RNA genome of human and simian immunodeficiency viruses is flanked by an upstream hairpin structure Benjamin Berkhout Department of Human Retrovirology, Academic Medical Center, University of Amsterdam, PO Box 22700, 1100 DE Amsterdam, The Netherlands Received July 16, 1997; Revised and Accepted August 26, 1997 ABSTRACT PBS site. Such additional contacts were originally proposed for Rous sarcoma virus (2–4), but similar interactions have been Reverse transcription of retroviral genomes is primed reported for human immunodeficiency virus type 1 (HIV-1) (5), by a tRNA molecule that anneals to an 18 nt primer HIV-2 (6) and several retrotransposon elements (7–9). Consistent binding site (PBS) on the viral RNA genome. Additional with the idea that regions of the viral genome outside the PBS base pair interactions between the tRNA primer and sequence participate in selective tRNA primer usage is the the viral RNA have been proposed. In particular, base observation that retroviruses mutated in the PBS are genetically pairing was proposed between the anticodon loop of unstable, as they revert to the wild-type PBS sequence after a few tRNALys3 and the ‘A-rich’ loop of a hairpin located passages in cell culture (10–13). On the other hand, reasonable immediately upstream of the PBS site in HIV-1 RNA. In transduction efficiencies were obtained with murine leukemia order to judge the importance of this sequence/structure virus vectors that use an unnatural or genetically engineered motif, we performed an extensive phylogenetic tRNA primer (14,15). -
Role of Reverse Transcriptase and APOBEC3G in Survival of Human
& My gy co lo lo ro g i y V Soni et al., Virol Mycol 2013, 3:1 Virology & Mycology DOI: 10.4172/2161-0517.1000125 ISSN: 2161-0517 Review Article Open Access Role of Reverse Transcriptase and APOBEC3G in Survival of Human Immune Deficiency Virus -1 Genome Raj Kumar Soni*, Amol Kanampalliwar and Archana Tiwari School of Biotechnology, Rajiv Gandhi Proudyogiki Vishwavidyalaya (State Technological University), Madhya Pradesh, India Abstract Development of an effective vaccine against HIV-1 is a major challenge for scientists at present. Rapid mutation and replication of the virus in patients contribute to the evolution of the virus, which makes it unconquerable. Hence a deep understanding of critical elements related to HIV-1 is necessary. Errors introduced during DNA synthesis by reverse transcriptase are the primary source of genetic variation within retroviral populations. Numerous current studies have shown that apolipo protein B mRNA-editing enzyme-catalytic polypeptide-like 3G (APOBEC3G) proteins mediated sub- lethal mutagenesis of HIV-1 proviral DNA contributes in viral fitness by accelerating human immunodeficiency virus-1 evolution. This results in the loss of the immunity and development of resistance against anti-viral drugs. This review focuses on the latest biological, biochemical, and structural studies in an attempt to discuss current ideas related to mutations initiated by reverse transcriptase and APOBEC3G. It also describes their effect on immunological diversity and retroviral restriction, and their overall effect on the viral genome respectively. A new procedure for eradication of HIV-1 has also been proposed based on the previous studies and proven facts. Keywords: HIV-1; Reverse transcriptase; Recombination; defined. -
Interactions Between APOBEC3 and Murine Retroviruses: Mechanisms of Restriction and Drug Resistance
University of Pennsylvania ScholarlyCommons Publicly Accessible Penn Dissertations 2013 Interactions Between APOBEC3 and Murine Retroviruses: Mechanisms of Restriction and Drug Resistance Alyssa Lea MacMillan University of Pennsylvania, [email protected] Follow this and additional works at: https://repository.upenn.edu/edissertations Part of the Virology Commons Recommended Citation MacMillan, Alyssa Lea, "Interactions Between APOBEC3 and Murine Retroviruses: Mechanisms of Restriction and Drug Resistance" (2013). Publicly Accessible Penn Dissertations. 894. https://repository.upenn.edu/edissertations/894 This paper is posted at ScholarlyCommons. https://repository.upenn.edu/edissertations/894 For more information, please contact [email protected]. Interactions Between APOBEC3 and Murine Retroviruses: Mechanisms of Restriction and Drug Resistance Abstract APOBEC3 proteins are important for antiretroviral defense in mammals. The activity of these factors has been well characterized in vitro, identifying cytidine deamination as an active source of viral restriction leading to hypermutation of viral DNA synthesized during reverse transcription. These mutations can result in viral lethality via disruption of critical genes, but in some cases is insufficiento t completely obstruct viral replication. This sublethal level of mutagenesis could aid in viral evolution. A cytidine deaminase-independent mechanism of restriction has also been identified, as catalytically inactive proteins are still able to inhibit infection in vitro. Murine retroviruses do not exhibit characteristics of hypermutation by mouse APOBEC3 in vivo. However, human APOBEC3G protein expressed in transgenic mice maintains antiviral restriction and actively deaminates viral genomes. The mechanism by which endogenous APOBEC3 proteins function is unclear. The mouse provides a system amenable to studying the interaction of APOBEC3 and retroviral targets in vivo. -
Criteria for Reverse Transcription Primer Binding
Criteria For Reverse Transcription Primer Binding Husain liquesce his calentures explores zealously, but tubuliflorous Markos never reft so inconvertibly. Irreproducible burgeonsGerome speeds out-of-bounds supernormally and snooze or swallows his Hounslow. illiberally when Wright is amphibrachic. Equine and sporocystic Urban always Transfer the plates to stop thermal cycler block. Reverse transcriptase activities and mechanism of action. DNA controlsequence from the soy lectin gene, however is expressed in both GM and unmodifiedsoy. This means that the reaction was established cell cycle of transcription primer. RT and PCR temperatures and times. In reverse transcription, transcripts were only and ic amplicons in cerebrospinal fluid from. Xeno Substrate. Dna for reverse transcription pcr system of microbiology community college of multiple forward primer. Method described as these transcription as well as when designing effective primer for sharing this sometimes leads to bind and biomedical laboratories starting amount can emerge in yeast. The truth is then cleaved by the polymerase enzyme during the reaction. Luna Universal qPCR & RT-qPCR NEB. Relative simplicity and structure of some of rt reaction buffer and labgown, which makes it is fully denatured proteins. In background amplification ofthe product into a nick, and assists with pcr include fever, such as chrome or specimens may vary widely reported primers. Relative quantitation of known target against common internal standard is particularly useful for control expression measurements. Transcription start site not long-range enhancers in the FK506 binding protein 5. PCR products can be detected using either fluorescent dyes that stray to. Oh of thereaction products which is a way to each standard deviation of cells participating in each primer hybridizes to study will enable reverse primer binding protein functions. -
Designing Lentiviral Vectors for Gene Therapy of Genetic Diseases
viruses Review Designing Lentiviral Vectors for Gene Therapy of Genetic Diseases Valentina Poletti 1,2,3,* and Fulvio Mavilio 4 1 Department of Woman and Child Health, University of Padua, 35128 Padua, Italy 2 Harvard Medical School, Harvard University, Boston, MA 02115, USA 3 Pediatric Research Institute City of Hope, 35128 Padua, Italy 4 Department of Life Sciences, University of Modena and Reggio Emilia, 41125 Modena, Italy; [email protected] * Correspondence: [email protected] Abstract: Lentiviral vectors are the most frequently used tool to stably transfer and express genes in the context of gene therapy for monogenic diseases. The vast majority of clinical applications involves an ex vivo modality whereby lentiviral vectors are used to transduce autologous somatic cells, ob- tained from patients and re-delivered to patients after transduction. Examples are hematopoietic stem cells used in gene therapy for hematological or neurometabolic diseases or T cells for immunotherapy of cancer. We review the design and use of lentiviral vectors in gene therapy of monogenic diseases, with a focus on controlling gene expression by transcriptional or post-transcriptional mechanisms in the context of vectors that have already entered a clinical development phase. Keywords: lentiviral vectors; transcriptional regulation; post-transcriptional regulation; miRNA; promoters; retroviral integration; ex vivo gene therapy Citation: Poletti, V.; Mavilio, F. 1. Introduction Designing Lentiviral Vectors for Gene Therapy of Genetic Diseases. -
Genome Walking with 2- to 4-Kb Steps Using Panhandle PCR
Downloaded from genome.cshlp.org on October 4, 2021 - Published by Cold Spring Harbor Laboratory Press Genome Walking with 2- to 4-kb Steps Using Panhandle PCR Douglas H. Jones and Stanley C. Winistorfer Department of Pediatrics, University of Iowa College of Medicine, Iowa City, Iowa 52242-1083 Panhandle PCR is a method that per- The PCR permits the highly specific trastrand annealing, those single strands mits the rapid retrieval of DNA seg- amplification of DNA sequences, but re- of genomic DNA that contain the com- ments flanking a known DNA se- quires knowledge of the sequences that plement to the 3' ends wil! form a stem- quence. This method extends the flank both ends of the sequence of inter- loop structure with a recessed 3' end. application of PCR to the retrieval of est. ~1-3~ Several methods have been de- Therefore, the ligated oligonucleotide DNA where only one end of the DNA veloped to permit the PCR amplification can prime template-directed DNA poly- sequence is known, so that one can of unknown DNA that flanks one end of merization on those strands that contain use PCR to walk along an uncharac- a known sequence. (4-23) The number the known sequence, resulting in known terized stretch of DNA. In this report, and variety of methods developed to am- DNA being attached to the uncharacter- we demonstrate that this method can plify DNA flanking a known sequence at- ized end of the unknown DNA (Fig. 1, be applied to the highly specific re- test to the scientific need for an effective step 3). -
HIV-1 RT/Dsrna Initiation Complex Prior to Nucleotide Incorporation
Structure of HIV-1 RT/dsRNA initiation complex prior to nucleotide incorporation Kalyan Dasa,b,c,d,1,2, Sergio E. Martineza,b,c,d,1, Jeffrey J. DeStefanoe,f, and Eddy Arnolda,b,2 aCenter for Advanced Biotechnology and Medicine, Rutgers University, Piscataway, NJ 08854; bDepartment of Chemistry and Chemical Biology, Rutgers University, Piscataway, NJ 08854; cRega Institute, The Katholieke Universiteit Leuven, 3000 Leuven, Belgium; dDepartment of Microbiology, Immunology and Transplantation, The Katholieke Universiteit Leuven, 3000 Leuven, Belgium; eDepartment of Cell Biology and Molecular Genetics, University of Maryland, College Park, MD 20742; and fMaryland Pathogen Research Institute, University of Maryland, College Park, MD 20742 Edited by John M. Coffin, Tufts University, Boston, MA, and approved February 19, 2019 (received for review August 16, 2018) The initiation phase of HIV reverse transcription has features that are Other tRNAs, when mutated to have sequence complementarity distinct from its elongation phase. The first structure of a reverse with PBS, are not efficient for HIV-1 reverse transcription initiation transcription initiation complex (RTIC) that trapped the complex after (9, 10). Posttranscriptional modifications of tRNALys3 have been incorporation of one ddCMP nucleotide was published recently reported to affect the formation of transcription initiation- [Larsen KP, et al. (2018) Nature 557:118–122]. Here we report a crystal competent vRNA–tRNALys3 complexes (11, 12). Nucleotide structure of a catalytically active HIV-1 RT/dsRNA complex that incorporation for initiation is almost 50-fold slower than that mimics the state of the RTIC before the first nucleotide incorporation. for reverse transcription elongation, and the processivity of RT The structure reveals that the dsRNA-bound conformation of RT is during elongation is four orders of magnitude higher in com- closer to that of RT bound to a nonnucleoside RT inhibitor (NNRTI) parison with initiation (13). -
Regulators of HIV-1 Reverse Transcription
Viruses 2009, 1, 873-894; doi:10.3390/v1030873 OPEN ACCESS viruses ISSN 1999-4915 www.mdpi.com/journal/viruses Review Reverse Transcriptase and Cellular Factors: Regulators of HIV-1 Reverse Transcription Kylie Warren 1,2, David Warrilow 1, Luke Meredith 1,3 and David Harrich 1,3,* 1 Division of Infectious Diseases, Queensland Institute of Medical Research, Brisbane, QLD, Australia; E-Mails: [email protected] (K.W.); [email protected] (D.W.); [email protected] (L.M.) 2 School of Natural Sciences, University of Western Sydney, Hawkesbury, NSW, Australia 3 Griffith Medical Research College, a joint program of Griffith University and the Queensland Institute of Medical Research, QIMR, Herston, QLD, 4006, Australia * Author to whom correspondence should be addressed; E-Mail: [email protected]; Tel.: +61-7-3845-36791; Fax: +61-7-3362-0107. Received: 30 September 2009; in revised form: 6 November 2009 / Accepted: 9 November 2009 / Published: 10 November 2009 Abstract: There is ample evidence that synthesis of HIV-1 proviral DNA from the viral RNA genome during reverse transcription requires host factors. However, only a few cellular proteins have been described in detail that affect reverse transcription and interact with reverse transcriptase (RT). HIV-1 integrase is an RT binding protein and a number of IN-binding proteins including INI1, components of the Sin3a complex, and Gemin2 affect reverse transcription. In addition, recent studies implicate the cellular proteins HuR, AKAP149, and DNA topoisomerase I in reverse transcription through an interaction with RT. In this review we will consider interactions of reverse transcription complex with viral and cellular factors and how they affect the reverse transcription process. -
The Development of Novel Str Miniplex Primer Sets for The
THE DEVELOPMENT OF NOVEL STR MINIPLEX PRIMER SETS FOR THE ANALYSIS OF DEGRADED AND COMPROMISED DNA SAMPLES A dissertation presented to the faculty of the College of Arts and Sciences of Ohio University In partial fulfillment of the requirements for the degree Doctor of Philosophy Denise T. Chung August 2004 This dissertation entitled THE DEVELOPMENT OF NOVEL STR MINIPLEX PRIMER SETS FOR THE ANALYSIS OF DEGRADED AND COMPROMISED DNA SAMPLES BY DENISE T. CHUNG has been approved for the Department of Chemistry and Biochemistry and the College of Arts and Sciences by Bruce R. McCord Associate Professor of Chemistry Leslie A. Flemming Dean, College of Arts and Sciences CHUNG, DENISE T. Ph.D. August 2004. Chemistry and Biochemistry The Development of Novel STR Miniplex Primer Sets for the Analysis of Degraded and Compromised DNA Samples (217 pp.) Director of Dissertation: Bruce R. McCord New Miniplex primer sets have been designed where the target sequence is much closer to the repeat region and will therefore produce smaller amplicons to facilitate the analysis of degraded DNA. The effectiveness of these primer sets on degraded DNA samples were tested on enzymatically degraded DNA, environmentally insulted bone samples, and real case work samples and compared to amplifications with a commercial multiplex kit. For both types of degraded DNA, the Miniplex primer sets were capable of producing more complete profiles due to the smaller amplicon size. A concordance study of 532 DNA samples was performed to check for the existence of potential point mutations in the Miniplex primer binding region or insertion/deletion between commercial primers and Miniplex primers. -
Target-Cell-Derived Trna-Like Primers for Reverse Transcription Support Retroviral Infection at Low Efficiency
Virology 297, 68–77 (2002) doi:10.1006/viro.2002.1380 View metadata, citation and similar papers at core.ac.uk brought to you by CORE provided by Elsevier - Publisher Connector Target-Cell-Derived tRNA-like Primers for Reverse Transcription Support Retroviral Infection at Low Efficiency Alexander Schmitz, Anders H. Lund,1 Anette C. Hansen, Mogens Duch, and Finn Skou Pedersen2 Department of Molecular and Structural Biology and Department of Medical Microbiology and Immunology, Aarhus University, C. F. Moellers Alle´, Building 130, DK-8000, Denmark Received September 25, 2001; returned to author for revision November 30, 2001; accepted January 4, 2002 Reverse transcription of a retroviral genome takes place in the cytoplasmof an infected cell bya process primed bya producer-cell-derived tRNA annealed to an 18-nucleotide primer-binding site (PBS). Byan assayinvolving primer comple- mentation of PBS-mutated vectors we analyzed whether tRNA primers derived from the target cell can sustain reverse transcription during murine leukemia virus (MLV) infection. Transduction efficiencies were 4–5 orders of magnitude below those of comparable producer-cell complementations. However, successful usage of a target-cell-derived tRNA primer was proven bycases of correction of single mismatches between Akv-MLV vectors and complementarytRNA primers toward the primer sequence in the integrated vector. Thus, target-cell-derived tRNA-like primers are able to initiate first-strand cDNA synthesis and plus-strand transfer leading to a complete provirus, suggesting that endogenous tRNAs from the infected cell mayalso have access to the intracellular viral complex at that step of the replication cycle. © 2002 Elsevier Science (USA) Key Words: murine leukemia virus; reverse transcription; primer-binding site; tRNA; target-cell complementation. -
Antiviral Mechanism and Biochemical Basis of the Human APOBEC3 Family
MINI REVIEW ARTICLE published: 09 July 2012 doi: 10.3389/fmicb.2012.00250 Antiviral mechanism and biochemical basis of the human APOBEC3 family Mayumi Imahashi 1,2, Masaaki Nakashima1,3 andYasumasa Iwatani 1,2* 1 Clinical Research Center, National Hospital Organization Nagoya Medical Center, Nagoya, Japan 2 Graduate School of Medicine, Nagoya University, Nagoya, Japan 3 Graduate School of Engineering, Nagoya University, Nagoya, Japan Edited by: The human APOBEC3 (A3) family (A, B, C, DE, F,G, and H) comprises host defense factors Atsushi Koito, Kumamoto University, that potently inhibit the replication of diverse retroviruses, retrotransposons, and the other Japan viral pathogens. HIV-1 has a counterstrategy that includes expressing the Vif protein to Reviewed by: Harold Charles Smith, University of abrogate A3 antiviral function. Without Vif, A3 proteins, particularly APOBEC3G (A3G) and Rochester, USA APOBEC3F (A3F), inhibit HIV-1 replication by blocking reverse transcription and/or integra- Hiroshi Matsuo, University of tion and hypermutating nascent viral cDNA. The molecular mechanisms of this antiviral Minnesota, USA activity have been primarily attributed to two biochemical characteristics common to A3 *Correspondence: proteins: catalyzing cytidine deamination in single-stranded DNA (ssDNA) and a nucleic Yasumasa Iwatani, Laboratory of Infectious Diseases, Department of acid-binding capability that is specific to ssDNA or ssRNA. Recent advances suggest that Microbiology and Immunology, unique property of A3G dimer/oligomer formations, is also important for the modification Clinical Research Center, National of antiviral activity. In this review article we summarize how A3 proteins, particularly A3G, Hospital Organization Nagoya Medical inhibit viral replication based on the biochemical and structural characteristics of the A3G Center, 4-1-1 San-no-Maru, Naka-ku, Nagoya, Aichi 460-0001, Japan. -
HIV-1 Subtypes and 5'LTR-Leader Sequence Variants Correlate With
viruses Article HIV-1 Subtypes and 50LTR-Leader Sequence Variants Correlate with Seroconversion Status in Pumwani Sex Worker Cohort Raghavan Sampathkumar 1,2, Joel Scott-Herridge 2 ID , Binhua Liang 2,3, Joshua Kimani 1,4, Francis A. Plummer 1,2 and Ma Luo 1,2,* 1 Department of Medical Microbiology, University of Manitoba, Winnipeg, MB, R3E 0J9, Canada; [email protected] (R.S.); [email protected] (J.K.); [email protected] (F.A.P.) 2 National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, MB, R3E 3R2, Canada; [email protected] (J.S.-H.); [email protected] (B.L.) 3 Department of Biochemistry and Medical Genetics, University of Manitoba, Winnipeg, MB, R3E 0J9, Canada 4 Department of Medical Microbiology, University of Nairobi, Nairobi, Kenya * Correspondence: [email protected]; Tel.: +1-204-789-5072; Fax: +1-204-784-4835 Received: 13 October 2017; Accepted: 20 December 2017; Published: 23 December 2017 Abstract: Within the Pumwani sex worker cohort, a subgroup remains seronegative, despite frequent exposure to HIV-1; some of them seroconverted several years later. This study attempts to identify viral variations in 50LTR-leader sequences (50LTR-LS) that might contribute to the late seroconversion. The 50LTR-LS contains sites essential for replication and genome packaging, viz, primer binding site (PBS), major splice donor (SD), and major packaging signal (PS). The 50LTR-LS of 20 late seroconverters (LSC) and 122 early seroconverters (EC) were amplified, cloned, and sequenced. HelixTree 6.4.3 was employed to classify HIV subtypes and sequence variants based on seroconversion status. We find that HIV-1 subtypes A1.UG and D.UG were overrepresented in the viruses infecting the LSC (P < 0.0001).