Table of Contents
How To Use This Catalog ...... 2 Service Information ...... 3-4 Placing Orders - Shipping ...... 3 Miscellaneous Information ...... 4 Product Information ...... 5-17 Definitions ...... 5 Detergent Analysis ...... 6-7 Lipid-like Detergents ...... 8-9 FOS-CHOLINE® Detergents ...... 8 FOS-MEA® ...... 9 Miscellaneous ...... 9 Nonionic Detergents ...... 10-14 Sugar-based Detergents ...... 10-11 Detergents Designed for Membrane Proteins ...... 12 Polyoxyethylene Detergents ...... 13-14 Sherpas® - Polymeric Solubilization Aids ...... 15 Biochemicals ...... 16-17 MTS Reagents ...... 16 NDSB Reagents ...... 17 Books ...... 18 New Products ...... 19 Alphabetical Product Listing ...... 20-102 Kits ...... 103-109 Technical Data ...... 110-118 Detergent Solubility ...... 110 Detergent Grades ...... 111 Detergent Stability ...... 112 Detergent Properties Tables ...... 113-118 Indexes ...... 119-129 Catalog Number Index ...... 119-120 Chemical Name Index ...... 121-125 CAS Registry Number Index ...... 126-129
The image on the cover is a molecular surface representation of the 2.0 Å crystal structure of the catalytic core subunits of cytochrome c oxidase from Rhodobacter sphaeroides, color coded by electrostatic potential (blue, positive; red, negative), together with resolved detergent decylmaltosides, a maltose headgroup, and alkyl chains of either detergents or lipids (yellow). Cover image generously provided by Professor Shelagh Ferguson-Miller, Michigan State University.
CATALOG Anatrace is a wholly owned subsidiary of USB Corporation. 2006 EDITION
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Shipping and ordering information is given in the Service Information section on pages 3 and 4. Please note that we have changed our policy on shipping most detergents. Stability studies indicate that a cold pack is not necessary with most of the reagents we offer, including our high quality detergents (see page 112). For your convenience, we continue to ship most of our products by overnight service for our domestic customers and by air for our international customers.
The Product Information section on pages 5-17 gives data on both new and old prod- ucts. Look in this section for general information on detergent and other product areas.
The New Product section on page 19 lists all the products that have been added since our previous catalaog edition.
To locate a particular detergent or other reagent, please check the Alphabetical Product Listing on pages 20-102 or use one of the three indexes available (Catalog Number Index, pages 119-120; Chemical Name Index, pages 121-125 or CAS Registry Number Index, pages 126-129). Note that the Chemical Name Index lists a number of common names for many of our products. A search function is also available at www.anatrace.com.
Analytical procedures to determine Purity of our detergents (Detergent Analysis) are given on pages 6-7 and many detergent properties are listed in the Detergent Properties Tables on pages 113-118.
For the latest product updates and additions, please visit our web site at www.anatrace.com.
2 800-252-1280 • 419-891-3030 • FAX 419-891-3037 • www.anatrace.com Service Information Placing Orders
Telephone: Monday-Friday 8:00 AM - 4:30 PM (EST) Toll free in the U.S. 800-252-1280 Or call 419-891-3030 Mail: Anatrace, Inc. 434 West Dussel Drive Maumee, OH 43537 Fax: 419-891-3037 Email: [email protected] Internet: www.anatrace.com Terms No minimum is required when ordering and all orders will be shipped promptly, usually on the same day. Net 30 days. Bulk Orders Should you need more than the largest size offered in the catalog, there can be considerable savings by ordering in bulk. When ordering please request special pricing. Prices Although we make every effort to keep prices constant, the prices herein may change without notice. Credit Cards We accept VISA®, MasterCard®, and American Express.
Shipping
Domestic Orders—Shipping and Handling—$20.00 All orders will be shipped by overnight service. Orders received before 1:00 PM EST will generally be shipped the same day.
International Orders—Shipping and Handling—$50.00 All orders will be shipped by express air. Our international customers are responsible for all duties and taxes at their location.
Please Note: Most detergents and other reagents offered by Anatrace have been shown to be stable under normal shipping conditions and will NOT be shipped with either an ice pack or dry ice. See Detergent Stability on page 112.
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Product Use Anatrace products are supplied for laboratory use only. They are to be used for laboratory research and experiments only. They may not be used for drugs, cosmetics, pesticide products or any use other than stated above. Package Weight Unless otherwise specified, the package will contain at least the indicated amount and usually slightly more. The user is cautioned to always measure the required amount from the container. Quality It is our goal to always supply the finest quality products available anywhere. If you are not completely satisfied that the chemical supplied to you meets the specifications described herein, we will replace it. Return Policy Should you receive a product ordered from this catalog which is believed to be nonconforming, please return the material within 30 days. You must receive authorization from Anatrace before returning the product. Returned products that have been ordered in error or have been retained more than 30 days are subject to a 25% restocking fee. Custom Synthesis Our research staff will be pleased to work with you on the synthesis and purification of a novel detergent or other reagent for your individual research needs. Custom Ampuling and Reagent Preparation Do you have a reagent that you must prepare frequently? Isn't it a waste of valuable lab time doing such routine work? We at Anatrace are experienced with a wide range of reagents and are accustomed to preparing reagents whether or not they contain detergents. We can custom blend or prepare reagents, or you can supply the reagent. So consider giving Anatrace the opportunity to prepare your reagents.
- Orders as small as 100 ampules - Volumes from 0.1 mL to 10 mL per ampul - Each container flushed with inert gases, as requested - Maximum container height of 85 mm - Flame sealed - Silk screen, transparent plastic, or opaque labels - GMP procedures followed throughout Technical Support For detailed information and/or technical assistance call our toll free number during normal business hours: 800-252-1280. Inquiries can also be sent by Fax: 419-891-3037 or e-mail: [email protected].
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Definitions All concentrations listed as percent are weight/volume unless specifically stated. AGGREGATION NUMBER is the molecular weight of the micelle divided by the molecular weight of the detergent.
BSA is bovine serum albumin. PRODUCT INFORMATION CMC is the critical micelle concentration. [ ] is the Chemical Abstracts Registry Number (CAS Number). FW is the formula weight. PERCENT ALPHA is the percent alpha isomer measured by an HPLC method developed at Anatrace. μS is micro Siemens. FW avg. is the approximate, estimated FW for commercial detergents. NEW PRODUCTS
C-DODECAFOS, C-OCTAFOS, CYCLOFOS, CYGENT, CYPFOS, DIFOS, FOSFEN, and PMAL are trademarks of Anatrace, Inc. ANAGRADE, ANAMEG, ANAPOE, ANZERGENT, C-HEGA, CYGLU, CYMAL, FOS-CHOLINE, FOS-MEA, HEGA, SHERPA, and SOL-GRADE are registered trademarks of Anatrace, Inc. BRIJ is a registered trademark of ICI Americas. COOMASSIE is a registered trademark of Imperial Chemical Industries, PLC. EMPIGEN BB is a registered trademark of Albright & Willson. GENAPOL is a registered trademark of Hoechst AG. LUBROL is a registered trademark of Imperial Chemical, Inc. PLURONIC is a registered trademark of BASF Corporation. THESIT is a registered trademark of Desitin Arzneimittel GmbH. TRITON is a registered trademark of Union Carbide Chemicals & Plastics. TRYCOL is a registered trademark of Henkel Corporation. TWEEN is a registered trademark of ICI Americas. ZWITTERGENT is a registered trademark of Calbiochem-Novabiochem Corporation. INDEX
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Detergent Analysis At Anatrace, we prepare nearly all of the more than 100 detergents listed in our catalog. Each lot is analyzed so that you the researcher can be assured of the highest consistent quality available anywhere. Nearly all of our ANAGRADE® detergents are purified chromatographically to be greater than 99% pure as measured by HPLC and to be low in UV absorbing or fluorescent impurities. We are pleased to list below the analytical procedures used to evaluate the detergents sold by Anatrace. Should you have any questions about these procedures, please feel free to contact us. Measurement of Purity (HPLC) ®
PRODUCT INFOMATION Nearly all detergents sold as ANAGRADE quality are greater than 99% pure as deter- mined by HPLC. SOL-GRADE® detergents are greater than 97% or 98% pure. The column used is a standard C18 column (4.6 mm x 250 mm) in conjunction with an RI detector. An eluant of either acetonitrile/water or methanol/water is acceptable. The ratio will vary depending on the hydrophobicity of the detergent. Some examples are given below: DETERGENT Acetonitrile/water Methanol/water
NEW PRODUCTS n-Heptyl-β-D-glucopyranoside 25/75 55/45 n-Nonyl-β-D-glucopyranoside 35/65 55/45 n-Hexyl-β-D-maltopyranoside 20/80 40/60 n-Octyl-β-D-maltopyranoside 30/70 55/45 n-Nonyl-β-D-maltopyranoside 35/65 60/40 n-Dodecyl-β-D-maltopyranoside 45/55 75/25 n-Tridecyl-β-D-maltopyranoside 60/40 75/25 n-Hexadecyl-β-D-maltopyranoside 70/30 85/15
PRODUCT LISTING FOS-CHOLINE®-10 45/55 65/35 FOS-CHOLINE®-12 45/55 75/25 FOS-CHOLINE®-14 45/55 85/15 CYMAL®-3 35/65 75/45 CYMAL®-5 45/55 65/35 Some impurities may be less than one percent and still affect the properties of a detergent lot. Therefore, the following tests are also performed to insure that you receive the highest quality of detergent reagent available anywhere. Absorbance The absorbance of the detergent solution (1% w/v unless otherwise stated) in water is measured in the UV region. Glucopyranosides and maltopyranosides should have low absorbance throughout this region. Some detergents such as C-HEGA®, HEGA® and MEGA detergents have high absorbance at 225 nm. INDEX
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Fluorescence The fluorescence of the detergent solution is compared to a standard BSA solution (0.1%)
unless otherwise stated. The excitation wavelength is 280 nm and the emission is measured PRODUCT INFORMATION at 345 nm. A very low level of impurity can still result in a large fluorescence background. Conductance For those detergents which are nonionic or zwitterions, a solution of detergent should have conductance nearly the same as deionized water. Measurement of conductance gives a very sensitive test for the presence of ionic impurities. Solubility in water Many of the impurities in detergent preparations are not soluble in water. Therefore cloudiness of a detergent solution at a concentration where it is known to be soluble
indicates the presence of an insoluble impurity. NEW PRODUCTS Measurement of pH The pH of the detergent solution at an appropriate concentration is measured. The pH should be neutral for detergents that are either uncharged or a zwitterion. Alcohol contamination Glucoside and maltoside detergents are prepared from the corresponding hydrophobic alcohol. Trace amounts of this alcohol in the detergent lot can cause cloudiness in the detergent solution. Therefore, we measure the amount of alcohol in each lot of detergent. A 25 to 50% solution of detergent in methanol is injected into an HPLC system as de- scribed under Measurement of Purity (HPLC). The ratio of acetonitrile to water may vary depending on the hydrophobicity of the alcohol. Alpha isomer Glucoside and maltoside detergents have two isomeric forms-α and β. Each β detergent is analyzed for the percent α isomer present. A sample of detergent is injected into an HPLC system as described under Measurement of Purity (HPLC). For this analysis the ratio of acetonitrile to water is lower so that the detergent is retained longer and the separation of α and β isomers is accomplished. The ratio of solvents for separation will vary depending on the condition of the column and the detergent being analyzed. Lot analysis, shipping and storage Every lot of detergent sold by Anatrace will be shipped with an analysis sheet listing the results of the appropriate tests described above. All detergents are high purity products shipped to you by overnight delivery. ANAPOE® detergents should be stored refrig- erated in the dark. All other detergents should be stored frozen and kept dry. Warm to room temperature before opening the container. INDEX
800-252-1280 • 419-891-3030 • FAX 419-891-3037 • www.anatrace.com 7 Product Information Lipid-like Detergents Anatrace is pleased to offer more than twenty phospholipid analogs. Support for the development effort came from NIDDK through a Small Business Innovative Research (SBIR) grant (1R43DK49911-01, 2R44DK049911-02, and 5R44DK049911-03).
FOS-CHOLINE® detergents The detergents in this series have a phosphocholine headgroup, but unlike phospholipids they possess simple hydro- phobic tails. n = 7, FOS-CHOLINE®-8 n = 8, FOS-CHOLINE®-9 O n = 9, FOS-CHOLINE®-10 + n = 10, FOS-CHOLINE®-11 CH3 (CH2 )n O P O (CH2 )2 N(CH 3 )3 n = 11, FOS-CHOLINE®-12 _ n = 12, FOS-CHOLINE®-13 PRODUCT INFOMATION O n = 13, FOS-CHOLINE®-14 n = 14, FOS-CHOLINE®-15 n = 15, FOS-CHOLINE®-16
The lack of a complex glycerol ester chain allows these detergents to be prepared and offered to the researcher at moderate prices, compared to many phospholipids. Note also that there are no chiral centers in these detergents as there are in the phospholipids and most phospholipid derivatives. FOS-CHOLINE®-12 (n-dodecylphosphocholine) 1-4
NEW PRODUCTS micelles have proven to be extremely useful as a medium in which to conduct NMR studies of membrane proteins and have also been shown to play a crucial role in refolding a misfolded membrane protein.5
FOS-CHOLINE®-ISO-9, FOS-CHOLINE®-ISO-11 To the phospholipid analog detergents discussed above, Anatrace has added FOS-CHOLINE®-ISO-9 and FOS- CHOLINE®-ISO-11 which possess a split hydrophobic tail packing more hydrophobicity into a shorter effective chain length. This detergent may be useful for the stabilization of proteins during crystallization.
CH3
PRODUCT LISTING O
CH CH (CH2)n 3 + n = 1, FOS-CHOLINE®-ISO-9 CH N(CH ) OPO (CH2)2 3 3 n = 2, FOS-CHOLINE®-ISO-11 CH3 CH (CH2)n _ O CH3
FOS-CHOLINE®-UNSAT-11-10 FOS-CHOLINE®-UNSAT-11-10 contains an eleven carbon alkyl tail with a double bond at the end of the chain:
O + CH2 CH (CH2)9 O PO(CH2)2 N(CH3)3
INDEX _ O
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FOS-MEA® detergents The FOS-MEA® detergents have a secondary amine in the head group instead of the quaternary amine found in the FOS-CHOLINE® detergents: PRODUCT INFORMATION O H n = 7, FOS-MEA®-8 CH3(CH2)n O P O (CH2)2 N CH3 n = 9, FOS-MEA®-10 O H n = 11, FOS-MEA®-12
Because of the replacement of the quaternary amine with a secondary amine, this series has a lower solubility than the FOS-CHOLINE® detergents.
™ CYCLOFOS detergents NEW PRODUCTS CYCLOFOS™ detergents combine the phosphocholine headgroup with an aliphatic tail containing a cyclohexyl group as present in the CYMAL® series of detergents.
n = 2, CYCLOFOS™-2 O ™ + n = 3, CYCLOFOS -3 ™ (CH2)n OPO(CH2)2 N(CH3)3 n = 4, CYCLOFOS -4 _ n = 5, CYCLOFOS™-5 O n = 6, CYCLOFOS™-6 n = 7, CYCLOFOS™-7
FOSFEN™-9 FOSFEN™-9 combines the phosphocholine headgroup with a phenyl ring-containing hydrophobic tail.
O + CH3 (CH2)n O P O (CH2)2 N(CH3)3 _ O
Note: FOSFEN™-9 is prepared from a technical grade mixture of monoalkylphenols, predominantly para substituted. For more information see Merck Index 12,6775.
1. C. Fares, D. S. Libich, G. Harauz, FEBS J. 273, 601-614 (2006). 2. K. Oxenoid and J. J. Chou, Proc. Natl. Acad. Sci. USA 102, 10870-10875 (2005). 3. K. Choowongkomon, C. R. Carlin and F. D. Sonnichsen, J. Biol. Chem. 280, 24043-24052 (2005). 4. K. Oxenoid, F. D. Soennichsen and C. R. Sanders, Biochemistry 40, 5111-5118 (2001). 5. B. Gorzelle, J. K. Nagy, K. Oxenoid, W. L. Lonzer, et al, Biochemistry 38, 16373-16382 (1999). INDEX
800-252-1280 • 419-891-3030 • FAX 419-891-3037 • www.anatrace.com 9 Product Information Nonionic Detergents Most of the detergents useful in the extraction and purification of membrane proteins are nonionic detergents which possess either a sugar based or a polyoxyethylene head group. Sugar-based detergents Sugar-based detergents are among the most popular type of detergents used with membrane proteins. The alkyl glucopyranoside series is available from the six carbon chain tail to dodecyl-β-D-glucopyranoside. The somewhat less polar thioglycoside analogs of some of these compounds are also available.
CH 2OH n = 5, hexyl−β−D-glucopyranoside n = 6, heptyl−β−D-glucopyranoside O O (CH 2) n CH 3 n = 7, octyl−β−D-glucopyranoside OH n = 8, nonyl−β−D-glucopyranoside n = 9, decyl−β−D-glucopyranoside PRODUCT INFOMATION HO n = 11, dodecyl−β−D-glucopyranoside OH
The high CMC value of hexyl-β-D-glucopyranoside (0.25M) indicates that a detergent with a shorter chain would typically not be useful. Decyl-β-D-glucopyranoside has a reasonable CMC value, but is marginally soluble. Dodecyl-β-D-glucopyranoside is even less soluble. For many membrane proteins, a member of the maltopyranoside series is milder and more effective than the compa-
NEW PRODUCTS rable member of the glucopyranoside series. n = 5, hexyl−β−D-maltopyranoside n = 7, octyl−β−D-maltopyranoside n = 8, nonyl−β−D-maltopyranoside CH 2OH CH 2OH n = 9, decyl−β−D-maltopyranoside O O O (CH 2) n CH 3 n = 10, undecyl−β−D-maltopyranoside n = 11, dodecyl D-maltopyranoside OH OH −β− n = 12, tridecyl−β−D-maltopyranoside HO O n = 13, tetradecyl−β−D-maltopyranoside OH OH n = 15, hexadecyl−β−D-maltopyranoside PRODUCT LISTING
Another advantage of the maltopyranoside head group is the increased solubility of the higher members of this series compared to the glucopyranoside series. Even hexadecyl-β-D-maltopyranoside is soluble although it must be heated to 50oC for a few minutes to make a “true” solution. The entire series of maltopyranoside detergents are available from hexyl-β-D-maltopyranoside to hexadecyl-β-D-maltopyranoside, except for the pentadecyl member. Since the maltopyranoside detergents are generally milder than the glucopyranoside detergents, one might think that maltotrioside detergents might be even more compatible with membrane proteins. Detergents with even larger head groups seem to be impractical because the higher polysaccharides are not available in pure form at a reason- able price. Two sugar-based detergents that have been available for some time are sucrose monododecanoate and sucrose monodecanoate. As with the maltose-based detergent series, these detergents are mild and useful for the extrac- tion of membrane proteins. The researcher should be aware, however, that these detergents are esters rather than glycosidic compounds and thus are easily hydrolyzed in acid, base or by proteases. The sucrose ester detergents INDEX are also mixtures of isomers in which the aliphatic ester is linked to any one of the three primary alcohols in the sucrose structure.
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O n = 10, sucrose monododecanoate n = 8, sucrose monodecanoate CH3 (CH 2 )n COCH2 O HOCH 2 O
OH OH O OH CH2 OH OH
OH PRODUCT INFORMATION Please note: Sucrose monoalkyl ester is a mixture of the three possible primary alcohol esters. Only one structure is shown here. The MEGA detergents are another series of glucose derivatives with an amide linkage between the alkyl tail and the glycoside head group:
O
CH3 (CH2)n C N CH3 n = 6, MEGA-8 n = 7, MEGA-9 CH2 n = 8, MEGA-10 H C OH NEW PRODUCTS HO C H
H C OH
H C OH
CH2OH
These detergents have been available for twenty years and have been used in numerous studies.1-6 As sugar-based detergents, they are rather easy to prepare and therefore reasonably priced. Unfortunately, their solubility is low, except for MEGA-8, and decreases as the alkyl chain is lengthened.
To overcome the low solubility, Anatrace has introduced a similar series: HEGA® detergents
O
® CH3(CH2)n C N (CH2)2OH n = 6, HEGA -8 n = 7, HEGA®-9 CH2 n = 8, HEGA®-10 n = 9, HEGA®-11 H C OH
HO C H
H C OH
H C OH
CH2OH The presence of an additional hydroxyl group results in increased solubility.
1. M. . Churchward, R. H. Butt, J. C. Lang, et al, Proteome Sci. 3, 5 (2005). INDEX 2. C. Roberts, B. Bond, I. R. White and H. J. Herdon, J. Recept. Signal Transduct Res. 20, 167-186 (2000). 3. D. Choudhury, A. Thompson, V. Stojanoff, et al, Science 285, 1061-1066 (1999). 4. W. H. Yue, Y. P. Zou, L. Yu and C. A. Yu, Biochemistry 30, 2303-2306 (1991). 5. D. M. Cortes and E. Perozo, Biochemistry 36, 10343-10352 (1997). 6. M. Hanatani, K. Nishifuji, M. Futai and T. Tsuchiya, J. Biochem. 95, 1349-1353 (1984).
800-252-1280 • 419-891-3030 • FAX 419-891-3037 • www.anatrace.com 11 Product Information Detergents Designed for Membrane Proteins CYMAL® Detergents1 Anatrace has developed more than two dozen detergents designed specifically for the extraction, purification and crystallization of membrane proteins. Support for the effort described here came from NIH through the Small Business Innovative Research (SBIR) program (1R43DK043172-01, 2R44DK043172-02, and 5R44DK043172- 03). The first series made available was the CYMAL® detergents which have the formula:
CH2OH CH2OH
O O O (CH2)n OH OH O HO OH OH PRODUCT INFOMATION The CMC values for these detergents are higher than for the corresponding linear chains containing the same number of carbons. Viewed another way, the cyclohexyl tails pack more hydrophobicity into a shorter effective chain length. Unpublished data from Dr. J. E. Harlan and Professor R. M. Garavito (Michigan State University) suggest that detergents that have a shorter chain length, but higher hydrophobicity, are useful for stabilizing proteins during crystal- lization. NEW PRODUCTS
CMC (mM) PRODUCT LISTING
Length (Angstroms) Number of Carbon Atoms U Cyclic Detergents Q Aliphatic Detergents
A study published in late 1999 by the Dana Farber Cancer Institute and Harvard Medical School showed that from the patented CYMAL® group of detergents, CYMAL-5® may be the detergent of choice for separating and purifying G-protein coupled receptors.5
1. US Patent 5,674,987 and US Patent 5,763,586. 2. S. Askolin, J. P. Turkenburg, M. Tenkanen, et al, Acta Crystallogr. D Biol. Crystallogr. 60, 1903- 1905 (2004). 3. H. Katayama, T. Tabata, Y. Ishihama, et al, Rapid Commun. Mass Spectrom. 18, 2388-2394 (2004). 4. G. J. Babcock, M. Farzan, and J. Sodroski, J. Biol. Chem. 278, 3378-3385 (2005). INDEX 5. Mirzabekov, T., Bannert, N., Farzan, M., Hofmann, W., Kolchisky, P., Wu, Lu., Ayalt, R. and Sodroski, I., J. of Biological Chemistry 274, 28745-28750 (1999).
12 800-252-1280 • 419-891-3030 • FAX 419-891-3037 • www.anatrace.com Product Information SERVICE INFORMATION Polyoxyethylene Glycol Detergents ANAPOE® Detergents Most polyoxyethylene detergents used in the early studies of membrane proteins were developed years ago for large industrial applications. Today an increasing number of well defined, highly purified deter- gents, designed specifically for the extraction of membrane proteins in their active state are available. PRODUCT INFORMATION Detergents such as dodecyl-β-D-maltopyranoside and octyl-β-D-glucopyranoside have replaced many polyoxyethylene detergents. Yet for some proteins the polyoxyethylene detergents remain very useful— particularly for routine extraction procedures. Polyoxyethylene detergents are available in a variety of structures and under an often confusing list of trade names, such as TRITON®, TWEEN®, GENAPOL®, BRIJ®, THESIT®, LUBROL®, etc. In addition to the confusion caused by trade names, industrial detergents are often a mixture of closely related detergents that may vary from lot to lot. They may also contain additives and contaminants which result in undesirable effects during protein extraction. One such contaminant is an oxidizing agent.1 These oxidants can in-
crease in concentration upon aging. NEW PRODUCTS The “aging” of polyoxyethylene detergents results from the tendency of ethers to react with oxygen to form peroxides. Light accelerates this process.2-4 In samples of polyoxyethylene detergents, hydrogen peroxide and organic peroxides with a variety of structures may be found.2-4 The concentration of hydrogen perox- ide equivalent can be as high as 0.2%.5 Conversely, it may be found that the level of peroxides has not increased over time because as they form they react with more detergent molecules, resulting in the pres- ence of several undesirable derivatives.2 The presence of peroxides during extraction of membrane proteins has resulted in inactivation, activation and even fragmentation of biological materials.4 Sulfhydryl groups are readily oxidized by peroxides and such oxidation induces aggregation of acetylcholine receptors.6 Adding to the complexity, sulfhydryl oxidation is implicated in the transition from a conformation which has a high affinity for acetylcholine to a low affinity form. This transition has been noted during the purification process.7,8 Interference in biochemical analysis can also be caused by peroxides in detergent solutions. Lever has reported that the Kober reagent, used to measure estrogen, reacted with detergent solutions containing peroxides to give brown solutions even without heating.2 Peroxides are probably responsible for the high blanks noted by Heath and Tappel when measuring lipid peroxides in detergent-solubilized membrane components.9 Even protein determinations can be affected, as noted by Stutzenberger for COOMASSIE® blue dye-binding assay, bicinchoninic acid method and the Folin phenol method.10
1. Chang, H. W. and Bock, E., Anal. Biochem. 104, (1980), 112-117. 2. Lever, M., Anal. Biochem. 83, (1977), 274-284. 3. Miki, T. and Orii, Y., Anal. Biochem. 146, (1985), 28-34. 4. Jaeger, J., Sorensen, K. and Wolff, J., Biochem. Biophys. Methods 29, (1994), 77-81. 5. Ashani, Y. and Catravas, G., Anal. Biochem. 109, (1980), 55-62. 6. Chang, H. W., Proc. Nat. Acad. Sci. USA 71, (1974), 2113-2117. 7. O’Brien, R. D. and Gibson, R. E., ABB 169, (1975), 458-463. 8. Chang, H. W. and Neumann, E., Proc. Nat. Acad. Sci. USA 73, (1976), 3364-3368. 9. Heath, R. L. and Tappel, A. L., Anal. Biochem. 76, (1976), 184-191. INDEX 10. Stutzenberger, F. J., Anal. Biochem. 207, (1992), 249-254.
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ANAPOE® detergents have been chromatographically purified to contain less than 20 μM of equivalent peroxide in a 10% solution. Each detergent is supplied in a 10% aqueous solution under argon gas in amber ampules.
Please note: ANAPOE® detergents are prepared from industrial detergents which are often mixtures of closely related detergents. Even though ANAPOE® detergents are purified by chromatography, they are not necessarily homogenous. See ANAPOE® on pages 25-29 for pricing.
Cat. No. Description Cat. No. Description PRODUCT INFOMATION
® ® ® APT020 ANAPOE -20 (TWEEN 20) APO129 ANAPOE -C12E9 APB035 ANAPOE®-35 (BRIJ®-35) (Polyoxyethylene(9)dodecyl ether) ® APB058 ANAPOE®-58 (BRIJ®-58) AP1210 ANAPOE -C12E10 (Polyoxyethylene(10)dodecyl ether) APT080 ANAPOE®-80 (TWEEN® 80) APO138 ANAPOE®-C E APO106 ANAPOE®-C E 13 8 10 6 (Polyoxyethylene(8)tridecyl ether) (Polyoxyethylene(6)decyl ether) NEW PRODUCTS APND40 ANAPOE®-NID-P40 APO109 ANAPOE®-C E 10 9 ® ® (Polyoxyethylene(9)decyl ether) APX100 ANAPOE -X-100 (TRITON X-100) ® ® ® APX114 ANAPOE -X-114 (TRITON X-114) APO128 ANAPOE -C12E8 (Polyoxyethylene(8)dodecyl ether) APX305 ANAPOE®-X-305 (TRITON® X-305) APX405 ANAPOE®-X-405 (TRITON® X-405)
These ANAPOE® detergents are also available in kits: PRODUCT LISTING DSOL-ANAP1 ANAPOE® MASTER KIT (1 mL size) 30.50 Kit contains 1 mL of all 15 ANAPOE® detergents DSOL-ANP10 ANAPOE® MASTER KIT (10 mL size) 83.00 Kit contains 10 mL of all 15 ANAPOE® detergents
® DSOL-ANPC ANAPOE CyEx KIT (10 mL size) 51.50 ® ® ® Kit contains 10 mL of ANAPOE -35, ANAPOE -58, ANAPOE -C10E6, ® ® ® ANAPOE -C10E9, ANAPOE -C12E8, ANAPOE -C12E9, ® ® ANAPOE -C12E10, and ANAPOE -C13E8. DSOL-ANPT ANAPOE® TRITON/TWEEN KIT (10 mL size) 30.50 Kit contains 10 mL of ANAPOE®-X-100, ANAPOE®-X-114, ANAPOE®-X-305, ANAPOE®-X-405, ANAPOE®-20, and ANAPOE®-80. In each Kit detergents are supplied in a 10% aqueous solution under argon gas in amber ampules. INDEX
14 800-252-1280 • 419-891-3030 • FAX 419-891-3037 • www.anatrace.com Product Information SERVICE INFORMATION Sherpas® - Polymeric Solubilization Aids
PMAL™-C12 was the first of a series of new polymeric reagents to be offered by Anatrace. PMAL™-C12 and the series that followed were first prepared and characterized under an NIH-SBIR grant (1R43GM060071-01, 2R44GM060071-02, 5R44GM060071-3) and are classified as amphipols. These polymers have a unique way of solubilizing an integral membrane protein.1-4 Amphipols wrap themselves around the hydrophobic transmembrane domains of membrane proteins so that the hydrophobic side chains of the polymer stabilize the hydrophobic surface of the protein, while the polar side chains of the polymer confer aqueous solubility to the protein-amphipol complex. PRODUCT INFORMATION Recent papers describe the use of PMAL™-C12 for delivering complex integral membrane proteins (IMPs) into a membrane.5,6 Applications can be imagined where it would be desirable to add a purified membrane protein to lipid vesicles or biological mem- branes under conditions where the protein could spontaneously insert and adopt its functionally active state without lysing the target bilayer. These polymers appear to have some of the functions of the naturally occurring molecules called chaperones.7,8 Since they are synthetic polymers and not naturally occuring, the mechanism of transport may be different than chaperones. We are calling our synthetic transport aids Sherpas®, after the mountain people who transport items in difficult environments. See pages 87-88 in the Product Listing section for information on the series of PMAL™ detergents.
( CH CH CH CH ) NEW PRODUCTS 2 n x=7, PMAL™-C8 _ x=9, PMAL™-C10 O C CO2 (CH2)x x=11, PMAL™-C12 x=15, PMAL™-C16 CH3 NH
(CH2)3 + H3C N H
CH3
1. Tribet, C., Audebert, R. and Popot. J.-L., Proc. Nat. Acad. Sci 93, Amphipols: polymers that keep membrane proteins soluble in aqueous solution, 15047-15050 (1996). 2. Tribet, C., Audebert and R., Popot. J.-L., Langmuir 13, Stabilization of hydrophobic colloidal dispersions in water with amphiphilic polymers: application to integral membrane proteins, 5570-5576 (1997). 3. Tribert, C., Mills, D., Haiders, M. and Popot, J.-L., Biochemie 80, Scanning transmission electron microscopy study of the molecular mass of amphipol/cytochrome b6f complexes, 475-482 (1998). 4. Champeil, P., Menguy, T., Tribet, C., Popot, J.-L. and le Marie, M., J. Biol. Chem. 275, Interaction of amphipols with sarcoplasmic retitculum calcium Ca2+-ATPase, 18623-18637 (2000). 5. Nagy, J. K., Hoffmann, A. K., Keyes, M. H., Gray, D. N., Oxenoid K. and Sanders, C. R., FEBS Letters 501, Use of amphipathic polymers to deliver a membrane protein to lipid bilayers, 115-120 (2001). 6. Gorzelle, Bonnie M., Hoffmann, Amy Kuhn, Keyes, Melvin H., Gray, Don N., Ray, Dale G. and Sanders, Charles R., J. Am. Chem. Soc. 124, Amphipols Can Support the Activity of a Membrane Enzyme, 11594-11595 (2002). 7. Johnson, J. L. and Craig, E. A., Methods Enzymol. 351, Identifying functional interactions with molecular chaperones, 442-453 (2002). 8. Morello, J. P., Petaja-Repo, U. E., Bichet, D. G., and Bouvier, M., Trends Pharmacol. Sci. 21, Pharmacological chaperones: a new twist on receptor folding, 466-469 (2000). 9. Popot, J. L., Berry, E. A., Charvolin, D., Creuzenet, C., Ebel, C., Engelman, D. M., Flotenmeyer, M., Giusti, F., Gohon, Y., Hervi, P., Hong, Q., Lakey, J. H., Leonard, K., Shuman, H. A., Timmins, P., Warschawski, D. E., Zito, F., Zoonens, M., Pucci, B., Tribet, C., Cell. Mol. Life Sci. 60, Amphipols: polymeric surfactants for membrane biology research, 1559-1574 (2003). 10. Cuccia, L., Trends Biochem. Sci. 27, Amphipols: polymers that can keep membrane proteins active in aqueous solution, 550-550 (2002). 11. Martinez, K. L., Gohon, Y., Corringer, P. J., Tribet, C., Merola, F., Changeux, J. P., and Popot, J. L., FEBS Lett. 528, Allosteric
transitions of Torpedo acetylcholine receptor in lipids, detergent and amphipols: molecular interactions vs. physical INDEX constraints, 251-256 (2002). 12. Prata, C., Giusti, F., Gohon, Y., Pucci, B., Popot, J. L., and Tribet, C., Biopolymers 56, Nonionic amphiphilic polymers derived from this (hydroxymethyl)acrylamidomethane keep membrane proteins soluble and native in the absence of detergent, 77-84 (2000).
800-252-1280 • 419-891-3030 • FAX 419-891-3037 • www.anatrace.com 15 Product Information Biochemicals Anatrace also offers several biochemicals including MTS reagents and NDSB reagents as well as convenience reagents to aid the researcher. All of our biochemicals are of the finest quality and can be ordered in bulk quantities. MTS Reagents The ability of MTS (methanethiosulfonate) reagents to react very rapidly and specifically with cysteine groups allows the quantitative derivatization of transmembrane proteins. The modification of the functional group attached to the methylthiosulfonate results in a variety of sulfhydryl specific reagents that can probe the structure of a protein. For example, charged MTS reagents are soluble in water and insoluble in a hydrophobic environment. Reaction of charged MTS reagents indicates that the cysteine group is probably on or near the surface and not buried in the interior of the protein or membrane. Other MTS reagents contain a spin labeled or a fluorescent group to probe the protein/membrane environment. Using site directed mutagenesis, one can introduce cysteine residues, one at a time, along a continuous stretch of PRODUCT INFOMATION residues. Modification of residues, particularly with charged MTS reagents, gives information about the accessibility of the residue in the ion channel. This technique was pioneered by Arthur Karlin and coworkers. SCAM, or substi- tuted cysteine accessibility method, uses a small, polar, MTS reagent to modify each expressed mutant. The accessi- bility of the particular residue is inferred from the irreversible functional effects of these reagents. Anatrace is pleased to offer five charged MTS reagents:
A110MT 2-AMINOETHYL METHANETHIOSULFONATE HYDROBROMIDE (MTSEA)
FW 236.15 C3H9NO2S2 HBr NEW PRODUCTS M110MT METHYL METHANETHIOSULFONATE (MMTS)
FW 126.20 [2949-92-0] C2H6O2S2 O110MT (1-OXYL-2,2,5,5-TETRAMETHYL-D3-PYRROLINE-3-METHYL) METHANETHIOSULFONATE (MTSL)
FW 264.30 [N/A] C10H18NO3S2 S110MT SODIUM (2-SULFONATOETHYL) METHANETHIOSULFONATE (MTSES)
FW 236.18 C3H7NaO5S3 T110MT [2-(TRIMETHYLAMMONIUM)ETHYL] METHANE THIOSULFONATE BROMIDE (MTSET)
FW 278.24 C6H16BrNO2S2
PRODUCT LISTING For more information see the MTS reagents in the Alphabetical Product Listing section. 1. P. J. Britto, L. Knipling, P. McPhie, and J. Wolff, Biochem. J. 389, 549-558 (2005). 2. A. Diez-Sampedro, D. D. Loo, E. M. Wright, et al, Biochemistry 43, 13175-13184 (2004). 3. E. M. Hedin, K. Hult, O. G. Mouritsen, and P. Hoyrup, J. Biochem. Biophys. Methods 60, 117-138 (2004). 4. N. Naber, S. Rice, M. Matuska, R. D. Vale, et al, Biophys. J. 84, 3190-3196 (2003). 5. Zhang, H. and Karlin, A., Biochem. 36, 15856-15864 (1997). 6. Akabas, M. H., Stauffer, D. A., Xu, M. and Karlin, A., Science 258, 307-310 (1992). 7. Stauffer, D. A. and Karlin, A., Biochem. 33, 6840-6849 (1994). 8. Akabas, M. H., Kaufman, C., Archdeacon, P. and Karlin, A., Neuron 13, 919-927 (1994). 9. Kenyon, G. L. and Bruice, T. W. in Methods Enzymol. 47-Part E, 407-430 (1977).
The following MTS reagent kits are also available: MT399-SMTS 134.92 (Each kit contains 100 mg of MTSEA, MMTS, MTSES, and MTSET) MT399-LMTS 538.08 INDEX (Each kit contains 500 mg of MTSEA, MMTS, MTSES, and MTSET)
16 800-252-1280 • 419-891-3030 • FAX 419-891-3037 • www.anatrace.com Product Information SERVICE INFORMATION
NDSB Reagents The series of NDSBs has been found to aid in the solubilization of some proteins. All members of the series, which are zwitterionic over a pH range of 3-12 and are very water soluble, have the unique ability to reduce protein aggre- gation while stabilizing protein structure. Other applications include refolding and renaturation of denatured proteins, isoelectrofocusing, and capillary electrophoresis. In addition, NDSB compounds are of benefit in some protein PRODUCT INFORMATION crystallizations.
1. M. S. Willis, J. K. Hogan, P. Prabhakar, et al, Protein Sci. 14, 1818-1826 (2005). 2. N. Expert-Bezancon, T. Rabilloud, L. Vuillard, and M. E. Goldberg, Biophys. Chem. 100, 469-479 (2003). 3. Vuillard, L., Baalbaki, B., Lehmann, M., Norager, S., Legrand, P. and Roth, M., Journal of Crystal Growth 168, 150-154 (1996). 4. Vuillard, L., et al, Biochem. J. 305, 337 (1995). Anatrace is pleased to offer five NDSB reagents: ND195 NDSB-195 (Dimethylethylammoniumpropanesulfonate) NEW PRODUCTS
FW 195.3 C7H17NO3S ND201 NDSB-201 [(3-1-Pyridino)-1-propanesulfonate / 1-(3-Sulfopropyl)-pyridinium hydroxide, inner salt]
FW 201.2 C8H11NO3S ND211 NDSB-211 [Dimethyl(2-hydroxyethyl)ammonium-1-propanesulfonate, 98%]
FW 211.3 C7H17NO4S ND221 NDSB-221 [3-(1-Methylpiperidinium)-1-propanesulfonate]
FW 221.3 C9H19NO3S ND256 NDSB-256 (Dimethylbenzylammoniumpropanesulfonate)
FW 257.4 C12H19NO3S For more information see the NDSB reagents in the alphabetical Product Listing section.
These NDSB reagents are also available in kits: ND399-1 (1g size) 41.45 (Each kit contains 1 g of NDSB-195, NDSB-201, NDSB-211, NDSB-221, and NDSB-256) ND399-5 (5g size) 181.13 (Each kit contains 5 g of NDSB-195, NDSB-201, NDSB-211, NDSB-221, and NDSB-256) INDEX
800-252-1280 • 419-891-3030 • FAX 419-891-3037 • www.anatrace.com 17 Books BOOKS Anatrace is pleased to offer the following texts to our researchers. You may order books separately or include them with your product order.
BOOK01 Methods and Results in Crystallization of Membrane Proteins 109.95 Edited by So Iwata Part I: Introduction Part II: Principles and Techniques in Membrane Protein Crystallization Part III: Examples of Successful Crystallization of Membrane Proteins Part IV: Crystallization Informatics of Membrane Proteins "Membrane protein crystallisation is still one of the hardest challenges in Structural Biology. This book...is written to assist the researchers attempting to crystallize their own membrane proteins." - from book Abstract (Introduction)
BOOK02 Membrane Protein Protocols: Expression, Purification, and Characterization 99.50 Edited by Barry S. Selinsky
PRODUCT INFOMATION A collection of key techniques for the study of receptors and transport proteins. The book provides examples of how different membrane proteins can be over-expressed in both prokaryotic and eukaryotic expression systems, how natural and overexpressed proteins can be solubilized from their host membranes, and how the solubilized protein can be purified in active form. Each protocol contains step-by-step instructions to ensure success, troubleshooting advice, lists of reagents, and tips on avoiding pitfalls.
BOOK03 Protein Structure and Function 49.95 Petsko and Ringe
NEW PRODUCTS This text introduces general principles of protein structure, folding, and function, then goes beyond these basics to tackle the conceptual basis of inferring structure and function from genomic sequence. Written for upper-level undergraduates and beginning graduate students, Protein Structure and Function will also be useful for working scientists needing an up-to-date introduction to the field. (Available in the U.S. and Canada only)
BOOK04 Structure & Mechanism in Protein Science: 119.00
A Guide to Enzyme Catalysis and Protein Folding Alan Fersht There is a new era of protein design, sparked by the convergence of protein folding and enzymology.
PRODUCT LISTING This book is a depiction of the principles of protein structure, activity, and mechanism today. It takes a more general look at mechanisms in protein science, emphasizing the unity of concepts in folding and catalysis and the importance of the relationships between basic chemistry, kinetics, thermodynamics, and structure. It makes protein engineering easier to understand and apply.
BOOK05 Proteins: Structures and Molecular Properties 102.00 T. E. Creighton In one convenient resource, Creighton’s landmark textbook offers an expert introduction to all aspects of proteins—biosynthesis, evolution, structures, dynamics, ligand binding, and catalysis. It works equally well as a reference or classroom textbook.
BOOK06 Introduction to Protein Structure 70.00 Branden and Tooze An up-to-date account of the principles of protein structure, with examples of key proteins in their biological context generously illustrated in full color. Introduces the general principles of protein structure and provides specific examples of proteins to show how they fulfil a wide variety of biological functions. Presents experimental approaches to determining and predicting protein structure, as well as engineering new proteins to modify their functions. INDEX
BKPKG1 Any Three Books 10% Discount
18 800-252-1280 • 419-891-3030 • FAX 419-891-3037 • www.anatrace.com New Products SERVICE INFORMATION NEW PRODUCTS
The following new Detergents are listed in this catalog: PRODUCT INFORMATION Detergent Catalog # Page #
ANAPOE®-NID-P40 APND40 24 NONIDET P40 SUBSTITUTE NIDP40 77
The following new Reagents are listed in this catalog:
Reagent Catalog # Page # NEW PRODUCTS
AMPICILLIN, SODIUM SALT A1000 20 DTNB D1000 55 DTE D2001 55 EDTA E1000 56 EGTA E1001 57 NEM E2000 57 GUANIDINE HYDROCHLORIDE G1000 67 IMIDAZOLE I1001 71 IPTG (PLANT), ANALYTICAL GRADE I1003P 72 LB BROTH, READY-MADE POWDER L1000 73 LITHUM DODECYL SULFATE (LDS) L1002 73 LURIA BROTH, READY-MADE POWDER L1001 74 MMTS M110MT 75 MTSL O110MT 76 SODIUM AZIDE S3000 89 TCEP T1000 92 TWEEN® 40 T1005 97 TWEEN® 60 T1006 97 UREA U1000 101 XGAL-IPTG B2050 102 INDEX
800-252-1280 • 419-891-3030 • FAX 419-891-3037 • www.anatrace.com 19 Product Listing
Catalog No. Product Name/Specifications Size Price
A110MT 2-AMINOETHYL METHANE THIOSULFONATE HYDROBROMIDE 100 mg 33.55 [MTSEA] 500 mg 134.20 1 g 241.56 • O FW 236.15 [16599-33-0] C3H9NO2S2 HBr Solubility in water at 0-5°C 10% . ≥ CH3 S S (CH2)2 NH2 HBr Positively charged reagent that reacts very rapidly and specifically with cysteine groups. O
Half-life (pH 7.0, 20°C) ~ 12.0 minutes2 Half-life (pH 6.0, 20°C) ~ 92.0 minutes2 Half-life (pH 7.0, 4°C) ~ 116.0 minutes2
1. Karlin, A. and Akabas, M. H., in Methods Enzymol. 293, 123-136 (1998). 2. Sobszak, I. and Lolkema, J. S., Biochem. 42, 9789-9796 (2003). PRODUCT INFOMATION
A1000 AMPICILLIN, SODIUM SALT 5 g 15.00 25 g 41.00 NH2 FW 371.39 [69-52-3] C16H18N3NaO4SH H H 100 g 127.00 Form: White Powder N S pH (10 mg/mL): 8.0-10.0 O N Water: ≤ 2.0% O Μethylene Chloride: ≤ 0.2% O NEW PRODUCTS NaO
A340 ANAMEG®-7, ANAGRADE® 1 g 36.93 [Methyl-6-O-(N-heptylcarbamoyl)-α-D-glucopyranoside / HECAMEG / 5 g 147.72 6-O-(N-Heptylcarbamoyl)-methyl-α-D-glucopyranoside] 25 g 590.89
FW 335.4 [115457-83-5] C H NO 15 29 7 O H CMC (H O) ~ 19.5 mM1 (0.65%) 2 Aggregation Number (H2O) ~ 92
PRODUCT LISTING CH2O C N (CH2)6 CH3 Purity ≥ 98% pure by HPLC analysis. O pH (1% solution) 5-9 OH Solubility in water at 0-5°C ≥ 10% Conductance (10% solution) < 80 μS HO OCH3 Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 OH Absorbance of a 1% detergent solution: 340 nm < 0.03 280 nm < 0.05 260 nm < 0.07 225 nm < 0.1 1. Plusquellec, D., Chevalier, G., Talibart, R. and Wroblewski, H., Anal. Biochem. 179, 145-153 (1989). INDEX
20 800-252-1280 • 419-891-3030 • FAX 419-891-3037 • www.anatrace.com Product Listing SERVICE INFORMATION
Catalog No. Product Name/Specifications Size Price
APT020 ANAPOE®-20 50 ml (50 ampules/1 ml each) 67.06 [TWEEN® 20 / Polyoxyethylene(20)sorbitan monolaurate / 50 ml (5 ampules/10 ml each) 55.88 Poly(oxy-1,2-ethanediyl) derivs., sorbitan monododecanoate] 100 ml (10 ampules/10 ml each) 100.58 500 ml (screw cap bottle) 403.30 FW avg. 1228 [9005-64-5] 1 HO(H2CH2CO)w CMC (H2O) ~ 0.059 mM (0.0072%) (OCH2CH2)xOH PRODUCT INFORMATION
(OCH CH ) OH O 2 2 y
O(CH2CH2O)zR Sum of w+x+y+z=20 OXIDANT-FREE R=C11H23C(O) Purified industrial detergent—may contain several components. Contains < 20 μM of equivalent peroxide. Supplied in a 10% (w/v) solution under argon gas.
1. Helenius, A., McCauslin, D. R., Fries, E. and Tanford, C., Methods Enzymol. 56, 743-749 (1979). NEW PRODUCTS
APB035 ANAPOE®-35 50 ml (50 ampules/1 ml each) 67.06 ® [BRIJ -35 / C12E23 / α-Dodecyl-ω-hydroxy-poly(oxy-1,2-ethanediyl / 50 ml (5 ampules/10 ml each) 55.88 Polyethylene glycol (23) monododecyl ether] 100 ml (10 ampules/10 ml each) 100.58 500 ml (screw cap bottle) 403.30
FW avg. 1198 [9002-92-0] (C2H4O)nC12H26O, n ~ 23 1 CMC (H2O) ~ 0.091 mM (0.011%) 2 Aggregation Number (H2O) ~ 40 HO(CH CH O) (CH ) CH OXIDANT-FREE 2 2 23 2 11 3 Purified industrial detergent—may contain several components. Contains < 20 μM of equivalent peroxide. Supplied in a 10% (w/v) solution under argon gas. 1. Helenius, A., McCauslin, D. R., Fries, E. and Tanford, C., Methods Enzymol. 56, 743-749 (1979). 2. le Maire, M., Champeil, P. and Moller, J. V., Biochim. Biophys. Acta 1508, 86-111 (2000).
APB058 ANAPOE®-58 50 ml (50 ampules/1 ml each) 67.06 ® [BRIJ -58 / C16E20 / α-Hexadecyl-ω-hydroxy-poly(oxy-1,2-ethanediyl / 50 ml (5 ampules/10 ml each) 55.88 Polyethylene glycol (20) monohexadecyl ether] 100 ml (10 ampules/10 ml each) 100.58 500 ml (screw cap bottle) 403.30
FW avg. 1122 [9004-95-9] (C2H4O)nC16H34O, n ~ 20 1 CMC (H2O) ~ 0.004 mM (0.00045%) OXIDANT-FREE HO(CH2CH2O)20 (CH2)15 CH3 Purified industrial detergent—may contain several components. Contains < 20 μM of equivalent peroxide. Supplied in a 10% (w/v) solution under argon gas.
1. For C16E21 listed in Helenius, A., McCauslin, D. R., Fries, E. and Tanford, C., Methods Enzymol. 56, 743-749 (1979). INDEX ANAPOE detergents are purified industrial detergents. Several homogenous polyoxyethylene detergents are available:
C8E4 (pg. 94), C8E5 (pg. 86), C8E6 (pg. 70), C10E5 (pg. 86), C12E8 (pg. 80).
800-252-1280 • 419-891-3030 • FAX 419-891-3037 • www.anatrace.com 21 Product Listing
Catalog No. Product Name/Specifications Size Price
APT080 ANAPOE®-80 50 ml (50 ampules/1 ml each) 67.06 [TWEEN® 80 / Polyoxyethylene(80)sorbitan monolaurate / 50 ml (5 ampules/10 ml each) 55.88 Poly(oxy-1,2-ethanediyl) derivs., (Z)-sorbitan mono-9-octadecanoate] 100 ml (10 ampules/10 ml each) 100.58 500 ml (screw cap bottle) 403.30 FW avg. 1310 [9005-65-6] CMC (H O) ~ 0.012 mM1 (0.0016%) HO(H2CH2CO)w 2 (OCH2CH2)xOH 2 Aggregation Number (H2O) ~ 58 (OCH CH ) OH O 2 2 y
O(CH2CH2O)zR
Sum of w+x+y+z=20 OXIDANT-FREE R=C17H33C(O) Purified industrial detergent—may contain several components.
PRODUCT INFOMATION Contains < 20 μM of equivalent peroxide. Supplied in a 10% (w/v) solution under argon gas. 1. Helenius, A., McCauslin, D. R., Fries, E. and Tanford, C., Methods Enzymol. 56, 743-749 (1979). 2. Black, Shaun D.: http://psyche.uthct.edu/shaun/SBlack/detergnt.html
® APO106 ANAPOE -C10E6 50 ml (50 ampules/1 ml each) 67.06 [Polyoxyethylene(6)decyl ether / 50 ml (5 ampules/10 ml each) 55.88 3,6,9,12,15,18-hexaoxaoctacosan-1-ol] 100 ml (10 ampules/10 ml each) 100.58
NEW PRODUCTS 500 ml (screw cap bottle) 403.30
FW avg. 423 [5168-89-8] (C2H4O)nC10H22O, n ~ 6 1 CMC (H2O) ~ 0.9 mM (0.038%) 2 Aggregation Number (H2O) ~ 40
OXIDANT-FREE CH3(CH2)9O(CH2CH2O)6H
Purified industrial detergent—C10E6 is the major component. Contains < 20 μM of equivalent peroxide. Supplied in a 10% (w/v) solution under argon gas. 1. Helenius, A., McCauslin, D. R., Fries, E. and Tanford, C., Methods Enzymol. 56, 743-749 (1979). 2. Black, Shaun D.: http://psyche.uthct.edu/shaun/SBlack/detergnt.html PRODUCT LISTING
® APO109 ANAPOE -C10E9 50 ml (50 ampules/1 ml each) 67.06 [Polyoxyethylene(9)decyl ether / 50 ml (5 ampules/10 ml each) 55.88 α-Decyl-ω-hydroxy-poly(oxy-1,2-ethanediyl] 100 ml (10 ampules/10 ml each) 100.58 500 ml (screw cap bottle) 403.30
FW avg. 555 [26183-52-81] (C2H4O)nC10H22O, n ~ 9 1 CMC (H2O) ~ 1.3 mM (0.072%)
OXIDANT-FREE CH3(CH2)9O(CH2CH2O)9H
Purified industrial detergent—C10E9 is the major component. Contains < 20 μM of equivalent peroxide. Supplied in a 10% (w/v) solution under argon gas. 1. Borchardt, J. K., Lab Products Notebook 7, No. 10, 20 (1996). INDEX
22 800-252-1280 • 419-891-3030 • FAX 419-891-3037 • www.anatrace.com Product Listing SERVICE INFORMATION
Catalog No. Product Name/Specifications Size Price
® APO128 ANAPOE -C12E8 50 ml (50 ampules/1 ml each) 67.06 [Polyoxyethylene(8)dodecyl ether / 50 ml (5 ampules/10 ml each) 55.88 3,6,9,12,15,18,21,24-octaoxahexatriacontan-1-ol] 100 ml (10 ampules/10 ml each) 100.58 500 ml (screw cap bottle) 403.30
FW avg. 539 [3055-98-9] (C2H4O)nC12H26O, n ~ 8 1,2 CMC (0.01 M TES, pH 7.5, 0.05 M NaCl, 0.1 mM CaCl2) ~ 0.09 mM (0.0048%) 3 PRODUCT INFORMATION Aggregation Number (H2O) ~ 123
OXIDANT-FREE CH3(CH2)11 O(CH2CH2O)8H
Purified industrial detergent—C12E8 is the major component. Contains < 20 μM of equivalent peroxide. Supplied in a 10% (w/v) solution under argon gas. 1. LeMaire, M., Kwee, S., Andersen, J. P., and Miller, J. V. Eur. J. Biochem., 129, 525-532, (1983). 2. LeMaire, M., Champeil, P., and Moller, J. V., Biochimica et Biophysica Acta 1508, 86-111 (2000). 3. Black, Shaun D.: http://psyche.uthct.edu/shaun/SBlack/detergnt.html
® APO129 ANAPOE -C12E9 50 ml (50 ampules/1 ml each) 67.06
[Polyoxyethylene(9)dodecyl ether / 50 ml (5 ampules/10 ml each) 55.88 NEW PRODUCTS α-Dodecyl-ω-hydroxy-poly(oxy-1,2-ethanediyl] 100 ml (10 ampules/10 ml each) 100.58 500 ml (screw cap bottle) 403.30
FW avg. 583 [3055-99-0] (C2H4O)nC12H26O n ~ 9 1 CMC (H2O) ~ 0.05 mM (0.003%)
OXIDANT-FREE CH3(CH2)11 O(CH2CH2O)9H
Purified industrial detergent—C12E9 is the major component. Contains < 20 μM of equivalent peroxide. Supplied in a 10% solution under argon gas. 1. Mast, R. C., and Haynes, L. V., J. Colloid Inerface Sci. 53, 35 (1975). 2. Rigler, P., Ulrich, W-P., Hovius, R., Ilegens, E., Pick, H., and Voegl, H., Biochemistry 42, 14017-14022 (2003).
® AP1210 ANAPOE -C12E10 50 ml (50 ampules/1 ml each) 67.06 [Polyoxyethylene(10)dodecyl ether / 50 ml (5 ampules/10 ml each) 55.88 3,6,9,12,15,18,24,27,30-decaoxadotetracontan-1-ol] 100 ml (10 ampules/10 ml each) 100.58 500 ml (screw cap bottle) 403.30
FW avg. 627 [6540-99-4] (C2H4O)nC12H26O, n ~ 10 1 CMC (H2O) ~ 0.2 mM (0.013%)
OXIDANT-FREE CH3(CH2)11 O(CH2CH2O)10 H
Purified industrial detergent—C12E10 is the major component. Contains < 20 μM of equivalent peroxide. Supplied in a 10% (w/v) solution under argon gas. 1. Mukerjee, P. and Mysels, K. J., NSRDS-NBS 36 (1970).
® APO138 ANAPOE -C13E8 50 ml (50 ampules/1 ml each) 67.06 [Polyoxyethylene(8)tridecyl ether] 50 ml (5 ampules/10 ml each) 55.88 100 ml (10 ampules/10 ml each) 100.58
FW avg. 553 [9043-30-5] (C2H4O)nC13H28O, n ~ 8 500 ml (screw cap bottle) 403.30 1 CMC (H2O) ~ 0.1 mM (0.0055%) INDEX OXIDANT-FREE CH3(CH2)12 O(CH2CH2O)8H
Purified industrial detergent—C13E8 is the major component. Contains < 20 μM of equivalent peroxide. Supplied in a 10% (w/v) solution under argon gas. 1. Borchardt, J. K., Lab Products Notebook 7, No. 10, 20 (1996).
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Catalog No. Product Name/Specifications Size Price
APND40 ANAPOE®-NID-P40 50 ml (50 ampules/1 ml each) 67.06 (Igepal CA-630 / [Octylphenoxy]polyethoxyethanol) 50 ml (5 ampules/10 ml each) 55.88 Nonidet P40 Substitute. 100 ml (10 ampules/10 ml each) 100.58 Chemically indistinguishable from Nonidet P40, which is 500 ml (screw cap bottle) 403.30 no longer commercially available. FW avg. 603 [2497-59-8] CMC (50mM Na+) ~ 0.05-0.3 mM1 Aggregation Number (H O)1 ~ 100-155 2 OH O OXIDANT-FREE 9 Purified industrial detergent—may contain several components. Contains < 20 μM of equivalent peroxide. Supplied in a 10% (w/v) solution under argon gas. 1. Black, Shaun D.: http://psyche.uthct.edu/shaun/SBlack/detergnt.html PRODUCT INFOMATION APX100 ANAPOE®-X-100 50 ml (50 ampules/1 ml each) 67.06 [TRITON® X-100 / 50 ml (5 ampules/10 ml each) 55.88 α-[4-(1,1,3,3-Tetramethylbutyl)phenyl]-ω-hydroxy- 100 ml (10 ampules/10 ml each) 100.58 poly(oxy-1,2-ethanediyl)] 500 ml (screw cap bottle) 403.30
FW avg. 647 [9002-93-1] t-Oct-C6H4-(OCH2CH2)XOH, x = 9-10 1-4 CMC (H2O) ~ 0.23 mM (0.015%) (w/v) 5 NEW PRODUCTS Aggregation Number (H2O) ~ 75-165 CH3 CH3 OXIDANT-FREE
Purified industrial detergent— CH3 C CH2 C (OCH2 CH2 )10 OH may contain several components. Contains < 20 μM of equivalent peroxide. CH3 CH3 Supplied in a 10% (w/v) solution under argon gas. 1. Vendittis, E., Paumbo, G., Parlata, G. and Borchini, U., Anal. Biochem. 115, 278-286 (1981). 2. Ross, S. and Oliver, J. P., J. Phys. Chem. 63, 1671-1674 (1959). 3. Mankovich, A. M., J. Amer. Oil Chem. Soc. 41, 449-452 (1964). 4. Rosenthal, K. S. and Koussale F., Anal. Chem. 55, 1115-1117 (1983). PRODUCT LISTING 5. LeMaire, M., Champeil, P. and Moller, J. V., Biochimica et Biophysica Acta 1508, 86-111 (2000).
APX114 ANAPOE®-X-114 50 ml (50 ampules/1 ml each) 67.06 [TRITON® X-114 / 50 ml (5 ampules/10 ml each) 55.88 α-[(1,1,3,3-Tetramethylbutyl)phenyl]-ω-hydroxy- 100 ml (10 ampules/10 ml each) 100.58 poly(oxy-1,2-ethanediyl)] 500 ml (screw cap bottle) 403.30
FW avg. 536 [9036-19-5] t-Oct-C6H4-(OCH2CH2)nOH, n ~ 7-8 1 CMC (H2O) ~ 0.2 mM (0.011%) (w/v) CH3 CH3 OXIDANT-FREE Purified industrial detergent— CH3 C CH2 C (OCH2CH2)8OH may contain several components. Contains < 20 μM of equivalent peroxide. CH3 CH3 Supplied in a 10% (w/v) solution under argon gas. 1. Rosenthal, K. S. and Koussale F., Anal. Chem. 55, 1115-1117 (1983). INDEX
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Catalog No. Product Name/Specifications Size Price
APX305 ANAPOE®-X-305 50 ml (50 ampules/1 ml each) 67.06 [TRITON® X-305 / α-[4-(1,1,3,3-Tetramethylbutyl)phenyl]- 50 ml (5 ampules/10 ml each) 55.88 ω-hydroxy-poly(oxy-1,2-ethanediyl)] 100 ml (10 ampules/10 ml each) 100.58 500 ml (screw cap bottle) 403.30 FW avg. 1526 [9002-93-1] t-Oct-C H -(OCH CH ) OH, n ~ 30 6 4 2 2 n CH3 CH3
OXIDANT-FREE PRODUCT INFORMATION
Purified industrial detergent— CH3 C CH2 C (OCH2CH2)30 OH may contain several components. Contains < 20 μM of equivalent peroxide. CH3 CH3 Supplied in a 10% (w/v) solution under argon gas.
APX405 ANAPOE®-X-405 50 ml (50 ampules/1 ml each) 67.06 [TRITON® X-405 / 50 ml (5 ampules/10 ml each) 55.88 α-[4-(1,1,3,3-Tetramethylbutyl)phenyl]-ω-hydroxy- 100 ml (10 ampules/10 ml each) 100.58 poly(oxy-1,2-ethanediyl)] 500 ml (screw cap bottle) 403.30 NEW PRODUCTS
FW avg. 1967 [9002-93-1] t-Oct-C6H4-(OCH2CH2)nOH, n ~ 40 1 CH3 CH3 CMC (H2O) ~ 0.81 mM (0.16%)
OXIDANT-FREE CH3 C CH2 C (OCH2 CH2 )40 OH Purified industrial detergent— CH CH may contain several components. 3 3 Contains < 20 μM of equivalent peroxide. Supplied in a 10% (w/v) solution under argon gas. 1. McPherson, A., CSHL Press, Crystallization of Biological Macromolecules, (1999).
AZ308 ANZERGENT® 3-8, ANALYTICAL GRADE 5 g 24.13 [n-Octyl-N,N-dimethyl-3-ammonio-1-propanesulfonate / 25 g 96.51 N,N-dimethyl-N-(3-sulfopropyl)-1-octaminium hydroxide, inner salt] 100 g 308.88
FW 279.6 [15178-76-4] C13H29NO3S 1 CMC (H2O) ~ 390 mM (10.9%) CH3 O Purity ≥ 98% pure by HPLC analysis. pH (1% solution) 5-8 + - CH3(CH2)7 N (CH2)3 S O Solubility in water at 20°C ≥ 30% Conductance (10% solution) < 70 μS CH3 O Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.02 280 nm < 0.04 260 nm < 0.06 225 nm < 0.1
ANZERGENT® 3-8 is chemically identical to ZWITTERGENT® 3-8. 1. Navarette, R. and Serrano, R., Biochim. Biophys. Acta 728, 403-408 (1983). INDEX
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Catalog No. Product Name/Specifications Size Price
AZ310 ANZERGENT® 3-10, ANALYTICAL GRADE 5 g 24.13 [n-Decyl-N,N-dimethyl-3-ammonio-1-propanesulfonate / 25 g 96.51 N,N-dimethyl-N-(3-sulfopropyl)-1-decanaminium hydroxide, inner salt] 100 g 308.88
FW 307.6 [15163-36-7] C15H33NO3S 1 CMC (H2O) ~ 39 mM (1.2%) 2 Aggregation Number (H2O) ~ 41 CH3 O Purity ≥ 98% pure by HPLC analysis. + - CH3(CH2)9 N (CH2)3 S O pH (1% solution) 5-8 Solubility in water at 20°C 30% ≥ CH O Conductance (10% solution) < 70 μS 3 Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.02 280 nm < 0.04 PRODUCT INFOMATION 260 nm < 0.06 225 nm < 0.1
ANZERGENT® 3-10 is chemically identical to ZWITTERGENT® 3-10. 1. Navarette, R. and Serrano, R., Biochim. Biophys. Acta 728, 403-408 (1983). 2. Black, Shaun D.: http://psyche.uthct.edu/shaun/SBlack/detergnt.html
®
NEW PRODUCTS AZ312 ANZERGENT 3-12, ANALYTICAL GRADE 5 g 24.13 [n-Dodecyl-N,N-dimethyl-3-ammonio-1-propanesulfonate / 25 g 96.51 N,N-dimethyl-1-N-(3-sulfopropyl)-1-dodecanaminium hydroxide, inner salt] 100 g 308.88
FW 335.5 [14933-08-5] C17H37NO3S CMC (20 mM Tris-HCl, pH 8.0, 0.1 M NaCl) ~ 2.8 mM1 (0.094%) Aggregation Number (H O)2 ~ 55-87 2 CH3 O Purity ≥ 98% pure by HPLC analysis. + - pH (1% solution) 5-8 CH3(CH2)11 N (CH2)3 S O
PRODUCT LISTING Solubility in water at 20°C ≥ 30% O Conductance (10% solution) < 70 μS CH3 Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.02 280 nm < 0.04 260 nm < 0.06 225 nm < 0.1
ANZERGENT® 3-12 is chemically identical to ZWITTERGENT® 3-12. 1. Vulliez-LeNormand, B. and Eisele, J. Anal. Biochem. 208, 241-243 (1993). 2. LeMaire, M., Champeil, P., and Moller, J. V., Biochimica et Biophysica Acta 1508, 86-111 (2000).
The zwitterionic head group of the ANZERGENT® Series is the same
INDEX head group for NDSB-195 and NDSB-256 (pg. 76).
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Catalog No. Product Name/Specifications Size Price
AZ314 ANZERGENT® 3-14, ANALYTICAL GRADE 5 g 24.13 [n-Tetradecyl-N,N-dimethyl-3-ammonio-1-propanesulfonate / 25 g 96.51 Dimethyl(3-sulfopropyl)tetradecyl-ammonium hydroxide, inner salt] 100 g 308.88
FW 363.6 [14933-09-6] C19H41NO3S 1 2 CMC (H2O) ~ 0.16 mM , CMC (10 mM Phosphate, pH 7.5)~ 0.2 mM (0.007%) 3
Aggregation Number (H2O) ~ 83-130 PRODUCT INFORMATION CH O Purity ≥ 98% pure by HPLC analysis. 3 pH (1% solution) 5-8 + - CH3(CH2)13 N (CH2)3 S O Solubility in water at 20°C ≥ 30%
Conductance (10% solution) < 70 μS CH3 O Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.02 280 nm < 0.04 260 nm < 0.06 225 nm < 0.1 NEW PRODUCTS ANZERGENT® 3-14 is chemically identical to ZWITTERGENT® 3-14. 1. Anatrace measurement. 2. Brito, R. M. M. and Vaz, W. L. C. Anal. Biochem. 152, 250-255 (1986). 3. LeMaire, M., Champeil, P., and Moller, J. V., Biochimica et Biophysica Acta 1508, 86-111 (2000).
B300 BIG CHAP, ANALYTICAL GRADE 1 g 67.60 [N,N'-bis-(3-D-Gluconamidopropyl)cholamide] 5 g 270.40 25 g 1081.60
FW 878.1 [86303-22-2] C42H75N3O16 1 CMC (H2O) ~ 2.9 mM (0.25%) 1 Aggregation Number (H2O) ~ 10 Purity ≥ 95% pure by HPLC analysis. pH (1% solution) 5-8 Solubility in water at 20°C ≥ 10% Conductance (10% solution) < 100 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.05 280 nm < 0.30 260 nm < 0.40 1. Hjelmeland, L. M., Klee, W. A. and Osborne, J. C., Anal. Biochem. 130, 485-490 (1983).
O O OH OH OH H CH 2 OH CH 3 N OH OH N CH N 3 OH OH OH H CH 2 OH OH CH 3 O
HO OH INDEX
Bulk pricing starts at as little as 4 times the largest catalog price. Call or email [email protected] for a quote.
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Catalog No. Product Name/Specifications Size Price
B310 BIG CHAP, DEOXY, ANALYTICAL GRADE 1 g 171.60 [N,N'-bis-(3-D-Gluconamidopropyl)deoxycholamide] 5 g 686.40
FW 862.1 [86303-23-3] C42H75N3O15 1 CMC (H2O) ~ 1.4 mM (0.12%) 1 Aggregation Number (H2O) ~ 8-16 Purity ≥ 95% pure by HPLC analysis. pH (1% solution) 5-8 Solubility in water at 20°C > 10% Conductance (10% solution) < 200 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.05 280 nm < 0.30 260 nm < 0.40 PRODUCT INFOMATION 1. Hjelmeland, L. M., Klee, W. A. and Osborne, J. C., Anal. Biochem. 130, 485-490 (1983). O O OH OH OH H CH 2 OH CH 3 N OH OH N CH N 3 OH OH OH H CH 2 OH OH CH 3 O
NEW PRODUCTS HO H
B2040 BLUE-GAL 50 mg 33.28 [5-Bromo-3-indolyl-β-D-galactopyranoside] 250 mg 124.80
H FW 374.2 [97753-82-7] C14H16BrNO6 N Purity ≥ 96% pure by HPLC analysis. Specific Rotation [α]20 (c=1, H O:DMF 1:1) -34° ± 3° CH 2OH D 2 Br
PRODUCT LISTING OH O Melting Point: 210-213°C O Solubility ≥ 2% in DMF OH IR spectrum conforms to specifications. OH
C640 C-DODECAFOS™, ANAGRADE® 1 g 135.51 [Cyclododecyl-1-phosphocholine] 5 g 542.06 25 g 2168.24
FW 349.17 [869549-38-2] C17H36NO4P CMC (H O) ~ 22 mM1 (0.77%) O 2 + (CH ) N(CH ) Purity ≥ 99% by HPLC analysis. O P O 2 2 3 3 _ pH (1% solution) 5-8 O Solubility in water at 0-5oC ≥ 20% Conductance (10% solution) < 200 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.02 INDEX 280 nm < 0.08 260 nm < 0.1 1. Anatrace measurement.
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Catalog No. Product Name/Specifications Size Price
C630 C-OCTAFOS™, ANAGRADE® 1 g 135.51 [Cyclooctyl-1-phosphocholine] 5 g 542.06 25 g 2168.24
FW 293.13 [869550-29-8] C13H28NO4P CMC (H O) ~ 450 mM1 (13%) O 2 + Purity ≥ 99% by HPLC analysis. O P O (CH2)2N(CH3)3 PRODUCT INFORMATION pH (1% solution) 5-8 _ O Solubility in water at 0-5oC ≥ 20% Conductance (10% solution) < 200 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.02 280 nm < 0.08 260 nm < 0.1 1. Anatrace measurement. NEW PRODUCTS CA100 CARBENICILLIN DISODIUM SALT 1 g 22.88 [α-Carboxybenzylpenicillin] 5 g 91.52 O 25 g 366.08
H S CH C NH CH3 FW 422.4 [4800-94-6] C17H16N2Na2O6S - CH3 O C N - IR spectrum conforms to specifications. O C O + Elemental analysis supplied with each lot. O Na + O Na
Carboxypenicillin antibiotic inhibits bacterial cell-wall synthesis (peptidoglycan cross-linking) by inactivating transpeptidases on the inner suface of the bacterial cell membrane. Can be used as a substitute for ampicillin at a similar concentration.
1. Perlman, D., Use of antibiotics in cell culture media, Meth. Enzymol., New York, NY (1979, Vol LVIII, 112. 2. Mandell, G., Antibacterial agents: Pencillins, cephalosporins, and other b-lactam antibiotics. The Pharmacological Basis of Therapeutics 9th ed., New York, NY (1996), 1074. 3. Raleigh, E.A., Escherichia coli, plasmids and bacteriophages, Short Protocols in Molecular Biology 2nd ed., New York, NY (1992), 1.
C314 3-CARBOXY-ω-HEPTADECENAMIDOPROPYL DIMETHYLAMINE, 1 g 25.48 ANALYTICAL GRADE 5 g 101.92 [N,N-Dimethyl-(3-carboxy-heptadec-16-ene)amidopropylamine] 25 g 407.68
FW 383.23 [869540-67-0] C H N O 23 44 2 3 OH 1 CMC (H2O) ~ < 0.001 mM (0.0004%) C=O O Purity ≥ 95% pure by HPLC analysis. CH3 pH (1% solution) 5-8 CH2 CH(CH2)12 CH CH2 CNH(CH2)3 N CH3 Solubility in water at 0-5°C ≥ 10% Conductance (10% solution) < 200 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 INDEX Absorbance of a 1% detergent solution: 340 nm < 0.05 280 nm < 0.1 1. Anatrace measurement.
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Catalog No. Product Name/Specifications Size Price
C385 3-CARBOXY-5-PENTADECENAMIDOPROPYL DIMETHYLAMINE, ANAGRADE® 1 g 35.36 [N,N-Dimethyl-(3-carboxy-pentadec-5-ene)amidopropylamine] 5 g 141.44 25 g 565.76
FW 368.5 [869547-63-7] C21H40N2O3 1 CMC (H2O) ~ 0.19mM (0.0070%) O C OH Purity ≥ 98% pure by HPLC analysis. CH3 pH (1% solution) 5-9 CH3(CH2)8CH CH CH2 CH CH2 C NH (CH2)3 N CH o 3 Solubility in water at 0-5 C ≥ 20% O Conductance (10% solution) < 100 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.1 280 nm < 0.2 260 nm < 0.3 1. Anatrace measurement. PRODUCT INFOMATION
C316 CHAPS, ANAGRADE® 1 g 12.30 [3-[(3-Cholamidopropyl)-dimethylammonio]-1-propane sulfonate / 5 g 49.18 N,N-Dimethyl-3-sulfo-N-[3-[[3α,5β,7α,12α)-3,7,12-trihydroxy-24-oxocholan-24-yl] 25 g 196.74 amino]propyl]-1-propanaminium hydroxide, inner salt] CH 3 FW 614.9 [75621-03-3] C32H58N2O7S O HO CH 3 CMC (H O) ~ 8 mM1 (0.49%) CH 2 3 + _ 2 N N SO Aggregation Number (H2O) ~ 10 CH 3 3 NEW PRODUCTS CH Purity ≥ 99% by HPLC analysis. H 3 pH (1% solution) 5-8 Solubility in water ≥ 0.5M HO OH Conductance (0.5 M solution) < 50 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.02 280 nm < 0.04 260 nm < 0.06 1. Hjelmeland, L. M., Nebert, D. W. and Osborne, Jr., J. C., Anal. Biochem. 130, 72-82 (1983). PRODUCT LISTING 2. Womack, M. D., Kendall, D. A. and MacDonald, R. C., Biochim. Biophys. Acta 733, 210-215 (1983). 3. Bellis, S. L., Kass-Simon, G. and Rhoads, D. E., Biochem. 31, 9838-9843 (1992).
C316S CHAPS, SOL-GRADE® 5 g 30.74 [3-[(3-Cholamidopropyl)-dimethylammonio]-1-propane sulfonate / 25 g 122.96 N,N-Dimethyl-3-sulfo-N-[3-[[3α,5β,7α,12α)-3,7,12-trihydroxy-24-oxocholan-24-yl] 100 g 393.47 amino]propyl]-1-propanaminium hydroxide, inner salt]
CH 3 FW 614.9 [75621-03-3] C H N O S O 32 58 2 7 HO CH 3 1 CH CMC (H2O) ~ 8 mM (0.49%) 3 + _ 2 N N SO 3 Aggregation Number (H2O) ~ 10 CH 3 CH Purity ≥ 97% pure by HPLC analysis. H 3 pH (1% solution) 5-8 Solubility in water ≥ 0.5M HO OH Conductance (0.5 M solution) < 200 μS Absorbance of a 1% detergent solution: 340 nm < 0.05 INDEX 280 nm < 0.1 260 nm < 0.2 See C316 for references.
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Catalog No. Product Name/Specifications Size Price
C317 CHAPSO, ANAGRADE® 1 g 34.98 [3-[(3-Cholamidopropyl)dimethylammonio]-2-hydroxy-1-propanesulfonate] 5 g 139.92 25 g 559.68
FW 630.9 [82473-24-3] C32H58N2O8S CH3 CMC (H O) ~8 mM1 (0.50%) O 2 HO CH 1 3 Aggregation Number (H O) ~ 11 CH3 OH 2 + _ PRODUCT INFORMATION N N CH SO 3 Purity ≥ 99% by HPLC analysis. 3 CH pH (1% solution) 5-8 H 3 Solubility in water 0.5M ≥ HO OH Conductance (0.5M solution) < 100 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.02 280 nm < 0.04 260 nm < 0.06 1. Hjelmeland, L. M., Nebert, D. W. and Osborne, Jr., J. C., Anal. Biochem. 130, 72-82 (1983). 2. Cladera, J., Rigaud, J., Villaverde, J. and Dunach, M., Eur. J. Biochem. 243, 798-804 (1997). 3. Sanders, C. R. II and Prestegard, J. H., Biophys. J. 58, 447-460 (1990). NEW PRODUCTS
C408 C-HEGA®-8, ANAGRADE® 1 g 98.80 [Cyclohexylethanoyl-N-hydroxyethylglucamide] 5 g 370.50 25 g 1389.38
FW 349.5 [603111-75-7] C16H31NO7 1 CMC (H2O) ~ 277 mM (9.7%) OH O Purity ≥ 99% by HPLC analysis. N pH (1% solution) 5-8 OH H Solubility in water at 20°C ≥ 20% OH Conductance (10% solution) < 100 μS H OH HO OH Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 H Absorbance of a 1% detergent solution: 340 nm < 0.05 H 280 nm < 0.08 260 nm < 0.1 1. Anatrace measurement.
C409 C-HEGA®-9, ANAGRADE® 1 g 98.80 [Cyclohexylpropanoyl-N-hydroxyethylglucamide] 5 g 370.50 25 g 1389.38
FW 363.5 [864434-14-0] C17H33NO7 1 CMC (H2O) ~ 108 mM (3.9%)
Purity ≥ 99% by HPLC analysis. OH pH (1% solution) 5-8 O Solubility in water at 20°C ≥ 20% N Conductance (10% solution) < 100 μS OH Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 H
OH INDEX Absorbance of a 1% detergent solution: 340 nm < 0.05 H OH HO OH 280 nm < 0.08 H 260 nm < 0.1 H 1. Anatrace measurement.
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Catalog No. Product Name/Specifications Size Price
C410 C-HEGA®-10, ANAGRADE® 1 g 103.74 [Cyclohexylbutanoyl-N-hydroxyethylglucamide]OH 5 g 414.96 O 25 g 1659.84
FW 377.5 [864434-15-1] C18H35NO7 N CMC (H O) ~ 35 mM1 (1.3%) OH 2 H OH Purity ≥ 99% by HPLC analysis. H OH pH (1% solution) 5-8 HO OH H Solubility in water at 20°C ≥ 20% H Conductance (10% solution) < 100 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.05 280 nm < 0.08 260 nm < 0.1 1. Anatrace measurement. PRODUCT INFOMATION 2. D. Choudhury, A. Thompson, V. Stojanoff, et al, Science 285, 1061-1066 (1999).
C411 C-HEGA®-11, ANAGRADE® 1 g 103.74 [Cyclohexylpentanoyl-N-hydroxyethylglucamide]OH 5 g 414.96 O 25 g 1659.84
FW 391.5 [864434-16-2] C19H37NO7 N CMC (H O) ~ 11.5 mM1 (0.45%) OH 2 H OH NEW PRODUCTS Purity ≥ 99% by HPLC analysis. H OH pH (1% solution) 5-8 HO OH H Solubility in water at 20°C ≥ 10% H Conductance (10% solution) < 100 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.05 280 nm < 0.08 260 nm < 0.1 1. Anatrace measurement. PRODUCT LISTING CH200 CHOLESTEROL 50 g 19.76 [3β-Hydroxy-5-cholestene / 5-cholesten-3β-ol] 250 g 59.28 1 kg 177.84
FW 386.64 [57-88-5] C27H46O
Melting point: 147-150°C Loss in drying ≤ 0.3% Residue on ignition ≤ 0.1% Solubility in alcohol 1% HO IR spectrum conforms to specifications.
All MSDS sheets are available on line at www.anatrace.com.
INDEX While on-line, check out our new products.
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CH210 CHOLESTERYL HEMISUCCINATE TRIS SALT 1 g 21.18 5 g 84.74 . FW 607.9 [102601-49-0] C31H50O4 C4H11NO3 25 g 338.96
Melting Point: 202-206°C
Appearance: White Powder PRODUCT INFORMATION
Solubility (6% aqueous CHAPS): 1.2% CH3 CH3
IR spectrum conforms to specifications. CH3 CH3
CH3 NH2 . O O HOCH2C CH2OH
HO C CH2CH2 CO CH2OH
Water soluble cholesterol standard: Klein, B., Kleinman, N. B. and Foreman, J. A., Clin. Chem. 20, 482-485 (1974). NEW PRODUCTS 1. Weiss, H. M. and Grisshammer, R., “Purification and characterization of the human adenosine A2a receptor functionally expressed in Escherichia coli”, Eur. J. Biochem. 269, 82-92 (2002). 2. Tucker, J. and Grisshammer, R., “Purification of a rat neurotensin receptor expressed in Escherichia coli”, Biochem. J. 317, 891-899 (1996). 3. Brown, P. J. and Schonbrunn, A., “Affinity Purification of a Somatostatin Receptor-G-protein Complex Demonstrates Specificity in Receptor-G-protein Coupling”, J. Biol. Chem. 268, No. 9, 6668-6676 (1993).
C3000 CMC DYE SOLUTION 10 ml (10 ampules/1 ml each) 30.16 [1mM aqueous solution of 8-Anilino-1-napthalene sulfonic acid / 50 ml (50 ampules/1 ml each) 120.64 8-(Phenylamino)-1-naphthalenesulfonic acid]
FW 299.35 [82-76-8] C16H13NO3S
NOTE: See Technical Bulletin #104 "CMC Determination by Dye Binding".
C6500 CULTURE TUBES 72 (6 x 50 mm) 25.99 - Borosilicate glass 720 (6 x 50 mm) 192.40
C508 CYCLOFOS™-2, ANAGRADE® 1 g 136.53 [2-Cyclohexyl-1-ethylphosphocholine] 5 g 546.12 25 g 2184.48 FW 293.8 [823796-65-2] C H NO P 13 28 4 O 1 CMC (H2O) ~ 256 mM (7.5%) + (CH2)2 OPO(CH2)2N(CH3)3 Purity ≥ 98% pure by HPLC analysis. _ pH (1% solution) 5-9 O Solubility in water at 0-5oC ≥ 20% Conductance (10% solution) < 200 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.05 280 nm < 0.08 INDEX 260 nm < 0.1 1. Anatrace measurement.
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Catalog No. Product Name/Specifications Size Price
C510 CYCLOFOS™-3, ANAGRADE® 1 g 136.53 [3-Cyclohexyl-1-propylphosphocholine] 5 g 546.12 25 g 2184.48
FW 306.91 [823796-66-3] C14H30NO4P 1 O CMC (H2O) ~ 43 mM (1.3%) + (CH2)3 OPO(CH2)2N(CH3)3 Purity ≥ 98% pure by HPLC analysis. _ pH (1% solution) 5-9 O Solubility in water at 0-5oC ≥ 20% Conductance (10% solution) < 200 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.05 280 nm < 0.08 260 nm < 0.1 1. Anatrace measurement. PRODUCT INFOMATION
C512 CYCLOFOS™-4, ANAGRADE® 1g 149.09 [4-Cyclohexyl-1-butylphosphocholine] 5 g 596.37 25 g 2385.45
FW 320.92 [675126-15-5] C15H32NO4P 1 O CMC (H2O) ~ 14 mM (0.45%) + (CH2)4 OPO(CH2)2N(CH3)3 Purity ≥ 98% pure by HPLC analysis. _ NEW PRODUCTS pH (1% solution) 5-9 O Solubility in water at 0-5oC ≥ 20% Conductance (10% solution) < 200 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.05 280 nm < 0.08 260 nm < 0.1 1. Anatrace measurement.
™ ®
PRODUCT LISTING C514 CYCLOFOS -5, ANAGRADE 1 g 149.09 [5-Cyclohexyl-1-pentylphosphocholine] 5 g 596.37 25 g 2385.45 FW 334.95 [657393-64-1] C H NO P 16 34 4 O CMC (H2O) ~ 4.5 mM (0.15%) + (CH2)5 OPO(CH2)2N(CH3)3 Purity ≥ 98% pure by HPLC analysis. _ pH (1% solution) 5-9 O Solubility in water at 0-5oC ≥ 20% Conductance (10% solution) < 200 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.05 280 nm < 0.08 260 nm < 0.1 INDEX
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Catalog No. Product Name/Specifications Size Price
C516 CYCLOFOS™-6, ANAGRADE® 1 g 171.60 [6-Cyclohexyl-1-hexylphosphocholine] 5 g 686.40 25 g 2745.60
FW 349.17 [657393-65-2] C17H36NO4P 1 O CMC (H2O) ~ 2.68 mM (0.094%) +
(CH2)6 OPO(CH2)2N(CH3)3 PRODUCT INFORMATION Purity ≥ 97% by HPLC analysis. _ pH (1% solution) 5-9 O Solubility in water at 0-5oC ≥ 20%. Conductance (10% solution) < 500 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.05 280 nm < 0.08 260 nm < 0.1 1. Anatrace measurement.
™ ®
C518 CYCLOFOS -7, ANAGRADE 1 g 192.40 NEW PRODUCTS [7-Cyclohexyl-1-heptylphosphocholine] 5 g 769.60 25 g 3078.40
FW 363.3 [657393-66-3] C18H38NO4P 1 O CMC (H2O) ~ 0.62 mM (0.022%) + (CH2)7 OPO(CH2)2N(CH3)3 Purity ≥ 97% pure by HPLC analysis. _ pH (1% solution) 5-9 O Solubility in water at 0-5oC ≥ 20% Conductance (10% solution) < 500 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.05 280 nm < 0.08 260 nm < 0.1 1. Anatrace measurement.
C702 CYGENT™ 1-3, ANAGRADE® 1 g 30.16 [1:1 mixture: N,N-Dimethyl-3(4-methyl-1-carboxy-2-cyclohexylamido) propylamine / 5 g 120.64 N,N-Dimethyl-3(4-methyl-2-carboxy-1-cyclohexylamido) propylamine] 25 g 482.56 O FW 269.4 g/mol [869796-48-5] C H N O 14 25 2 3 CH3 1 CH C NH (CH ) N CMC (H2O) > 500 mM 3 2 3 CH3 Purity ≥ 99% by HPLC analysis. pH (1% solution) 5-8 C OH
Solubility in water at 20°C ≥ 20% O Conductance (10% solution) < 100 μS O Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.1 CH3 C OH 280 nm < 0.3 260 nm < 0.5 CH3 C NH (CH2)3 N 1. Anatrace measurement. CH
3 INDEX O
CYGENT™ 1-3 is approximately a 1:1 mixture of the above.
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Catalog No. Product Name/Specifications Size Price
C323G CYGLU®-3, ANAGRADE® 1 g 70.70 [3-Cyclohexyl-1-propyl-β-D-glucoside] 5 g 264.59 25 g 994.21
FW 304.4 [869541-00-4] C15H28O6 1 CMC (H2O) ~ 28 mM (0.86%) CH2 OH Purity ≥ 99% by HPLC analysis. O (CH2 )3 O Percent alpha < 4 (HPLC) OH Percent cyclohexylpropanol < 0.005 (HPLC) pH (1% solution) 5-8 HO Solubility in water at 0-5°C ≥ 20% OH Conductance (10% solution) < 40 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.02 280 nm < 0.04 PRODUCT INFOMATION 260 nm < 0.06 225 nm < 0.1 1. Anatrace measurement.
C324G CYGLU®-4, ANAGRADE® 1 g 89.98 [4-Cyclohexyl-1-butyl-β-D-glucoside] 5 g 337.43 25 g 1265.36 FW 318.4 [869542-54-1] C H O
NEW PRODUCTS 16 30 2 1 CMC (H2O) ~ 1.8 mM (0.058%) CH2 OH Purity ≥ 99% by HPLC analysis. O (CH2 )4 Percent alpha < 4 (HPLC) O Percent (starting alcohol) < 0.005 (HPLC) OH pH (0.1% solution) 5-8 HO Solubility in water at 20°C > 0.1% OH Conductance (0.1% solution) < 40 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 0.1% detergent solution: 340 nm < 0.02 PRODUCT LISTING 280 nm < 0.04 260 nm < 0.06 225 nm < 0.08 1. Anatrace measurement.
Need to test several detergents? Check out our kits on pages 103-109. If you require a custom kit, please request a quote. INDEX
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Catalog No. Product Name/Specifications Size Price
C321 CYMAL®-1, ANAGRADE® 1 g 89.98 [Cyclohexyl-methyl-β-D-maltoside1] 5 g 337.43 25 g 1265.36
FW 438.5 [26080-46-46] C19H34O11 2 2 CMC (H2O) ~ 340 mM (15%) CMC (0.15M NaCl) ~ 360 mM PRODUCT INFORMATION Purity ≥ 99% by HPLC analysis. CH2OH CH2OH Percent alpha < 4 (HPLC) O O O CH2 Percent cyclohexylmethanol < 0.005 (HPLC) OH OH pH (1% solution) 5-8 O HO Solubility in water at 20°C 20% ≥ OH OH Conductance (10% solution) < 40 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.02 280 nm < 0.04 260 nm < 0.06
225 nm < 0.1 NEW PRODUCTS 1. US Patent 5,674,987 and US Patent 5,763,586. 2. Anatrace measurement.
C322 CYMAL®-2, ANAGRADE® 1 g 74.94 [2-Cyclohexyl-1-ethyl-β-D-maltoside1] 5 g 280.46 25 g 1053.86
FW 452.5 [260804-65-7] C20H36O11 2 2 CMC (H2O) ~ 120 mM (5.4%) CMC (0.15M NaCl) ~ 104 mM
Purity ≥ 99% by HPLC analysis. CH2OH CH2OH Percent alpha < 4 (HPLC) O O O (CH2)2 Percent cyclohexylethanol < 0.005 (HPLC) OH OH pH (1% solution) 5-8 O Solubility in water at 20°C ≥ 20% HO Conductance (10% solution) < 40 μS OH OH Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.02 280 nm < 0.04 260 nm < 0.06 225 nm < 0.1 1. US Patent 5,674,987 and US Patent 5,763,586. 2. Anatrace measurement.
Notes: INDEX
More information on the CYMAL series is available on page 12.
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Catalog No. Product Name/Specifications Size Price
C323 CYMAL®-3, ANAGRADE® 1 g 74.94 [3-Cyclohexyl-1-propyl-β-D-maltoside1] 5 g 280.46 25 g 1053.86
FW 466.5 [181135-58-0] C21H38O11 2 2 CMC (H2O) ~ 34.5 mM (1.6%) CMC (0.15M NaCl) ~ 29 mM 3 Aggregation Number (H2O) ~ 38 CH2OH CH2OH Purity ≥ 99% by HPLC analysis. O O (CH ) Percent alpha < 4 (HPLC) O 2 3 OH OH Percent cyclohexylpropanol < 0.005 (HPLC) O pH (1% solution) 5-8 HO Solubility in water at 20°C ≥ 20% OH OH Conductance (10% solution) < 40 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.02 PRODUCT INFOMATION 280 nm < 0.04 260 nm < 0.06 225 nm < 0.1 1. US Patent 5,674,987 and US Patent 5,763,586. 2. Anatrace measurement. 3. Anatrace measurement in collaboration with Professor R. M. Garavito (Michigan State University).
C324 CYMAL®-4, ANAGRADE® 1 g 89.98 NEW PRODUCTS [4-Cyclohexyl-1-butyl-β-D-maltoside1] 5 g 337.43 25 g 1265.36
FW 480.5 [181135-57-9] C22H40O11 2 2 CMC (H2O) ~ 7.6 mM (0.37%) CMC (0.15M NaCl) ~ 7.3 mM 2 Aggregation Number (H2O) ~ 45 CH2OH CH2OH Purity ≥ 99% by HPLC analysis. Percent alpha < 4 (HPLC) O O O (CH2)4 Percent cyclohexylbutanol < 0.005 (HPLC) OH OH O pH (1% solution) 5-8 HO PRODUCT LISTING Solubility in water at 20°C ≥ 20% OH OH Conductance (10% solution) < 40 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.02 280 nm < 0.04 260 nm < 0.06 225 nm < 0.1 1. US Patent 5,674,987 and US Patent 5,763,586. 2. Anatrace measurement in collaboration with Professor R. M. Garavito (Michigan State University).
On-line ordering available at INDEX www.anatrace.com
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Catalog No. Product Name/Specifications Size Price
C325 CYMAL®-5, ANAGRADE® 1 g 95.68 [5-Cyclohexyl-1-pentyl-β-D-maltoside1] 5 g 382.72 25 g 1530.88
FW 494.5 [250692-65-0] C23H42O11 2 2 CMC (H2O) ~ 2.4-5.0 mM (0.12%) CMC (0.15M NaCl) ~ 2.0 mM 2 Aggregation Number (H2O) ~ 66 PRODUCT INFORMATION CH2OH CH2OH Purity ≥ 99% by HPLC analysis. O Percent alpha < 4 (HPLC) O O (CH2)5 Percent cyclohexylpentanol < 0.005 (HPLC) OH OH O pH (1% solution) 5-8 HO Solubility in water at 20°C ≥ 20% OH OH Conductance (10% solution) < 40 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.02 280 nm < 0.04 260 nm < 0.06 NEW PRODUCTS 225 nm < 0.1 1. US Patent 5,674,987 and US Patent 5,763,586. 2. Anatrace measurement in collaboration with Professor R. M. Garavito (Michigan State University). 3. S. Askolin, J. P. Turkenburg, M. Tenkanen, et al, Acta Crystallogr. D Biol. Crystallogr. 60, 1903-1905 (2004). 4. H. Katayama, T. Tabata, Y. Ishihama, et al, Rapid Commun. Mass Spectrom. 18, 2388-2394 (2004). 5. D. Dorjsuren, Y. Badralmaa, J. Mikovits, et al, Protein Expr. Purif. 29, 42-50 (2003). 6. Wester, M. R., Johnson, E. F., Marques-Soares, C., Dansette, P. M., Mansuy, D., and Stout, C. D., Biochemisry 42, 6370-6379 (2003).
C325S CYMAL®-5, SOL-GRADE® 1 g 76.96 [5-Cyclohexyl-1-pentyl-β-D-maltoside1] 5 g 307.84 25 g 1231.36
FW 494.5 [250692-65-0] C23H42O11 2 2 CMC (H2O) ~ 2.4-5.0 mM (0.12%) CMC (0.15M NaCl) ~ 2.0 mM 2 Aggregation Number (H2O) ~ 66 CH OH CH2OH Purity ≥ 98% pure by HPLC analysis. 2 Percent alpha < 10 (HPLC) O O O (CH2)5 Percent cyclohexylpentanol < 0.05 (HPLC) OH OH O pH (1% solution) 4-9 HO Solubility in water at 20°C ≥ 20% OH OH Conductance (10% solution) < 100 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.05 280 nm < 0.1 260 nm < 0.1 225 nm < 0.2 See C325 for references. INDEX
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Catalog No. Product Name/Specifications Size Price
C326 CYMAL®-6, ANAGRADE® 1 g 122.72 [6-Cyclohexyl-1-hexyl-β-D-maltoside1] 5 g 490.88 25 g 1963.52
FW 508.5 [228579-27-9] C24H44O11 2 CMC (H2O) ~ 0.56 mM (0.028%) 2 CH2OH CH2OH Aggregation Number (H2O) ~ 63 O Purity ≥ 99% by HPLC analysis. O O (CH2)6 Percent alpha < 4 (HPLC) OH OH O Percent cyclohexylhexanol < 0.005 (HPLC) HO pH (1% solution) 5-8 OH OH Solubility in water at 20°C ≥ 20% Conductance (10% solution) < 40 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.02 PRODUCT INFOMATION 280 nm < 0.04 260 nm < 0.06 225 nm < 0.1 1. US Patent 5,674,987 and US Patent 5,763,586. 2. Anatrace measurement in collaboration with Professor R. M. Garavito (Michigan State University). 3. Nukaga, M., Abe, T., Venkatesan, A. M., Mansour, T. S., Bonomo, R. A., and Knox, J. R., Biochemistry 42, 13152-13159 (2003). 4. R. J. Guan, Y. Xiang, M. Wang, et al, Acta Crystallogr. D Biol. Crystallogr. 57, 1313-1315 (2001).
NEW PRODUCTS 5. Ostermeier, C., Harrenga, A., Ermler, U. and Michel, H., Proc. Natl. Acad. Sci. USA 94, 10547-10553 (1997).
C326LA CYMAL®-6, ANAGRADE® 1 g 174.72 [6-Cyclohexyl-1-hexyl-β-D-maltoside1] 5 g 698.88 (Contains almost no alpha isomer) 25 g 2795.52
FW 508.5 [228579-27-9] C24H44O11 2 CH OH CH OH CMC (H2O) ~ 0.56 mM (0.028%) 2 2 O Purity ≥ 99% by HPLC analysis. O O (CH2)6 OH OH PRODUCT LISTING Percent alpha < 0.5 (HPLC) O Percent cyclohexylhexanol < 0.005 (HPLC) HO pH (1% solution) 5-8 OH OH Solubility in water at 0-5°C ≥ 20% Conductance (10% solution) < 40 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.02 280 nm < 0.04 260 nm < 0.06 225 nm < 0.1 See C326 for references. INDEX
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Catalog No. Product Name/Specifications Size Price
C326S CYMAL®-6, SOL-GRADE® 1 g 98.80 [6-Cyclohexyl-1-hexyl-β-D-maltoside1] 5 g 395.20 25 g 1580.80
FW 508.5 [228579-27-9] C24H44O11 2 CMC (H2O) ~ 0.56 mM (0.028%) 2 CH OH CH OH 2 2 PRODUCT INFORMATION Aggregation Number (H2O) ~ 63 O Purity ≥ 98% pure by HPLC analysis. O O (CH2)6 Percent alpha < 10 (HPLC) OH OH O Percent cyclohexylhexanol < 0.05 (HPLC) HO pH (1% solution) 4-9 OH OH Solubility in water at 20°C ≥ 20% Conductance (10% solution) < 100 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.05 280 nm < 0.1 260 nm < 0.1 NEW PRODUCTS 225 nm < 0.2 See C326 for references.
C327 CYMAL®-7, ANAGRADE® 1 g 140.40 [7-Cyclohexyl-1-heptyl-β-D-maltoside1] 5 g 561.60 25 g 2246.40
FW 522.5 [349477-49-2] C25H46O11 2 CMC (H2O) ~ 0.19 mM (0.0099%) CH2OH CH2OH Purity ≥ 99% by HPLC analysis. O O O (CH2)7 Percent alpha < 4 (HPLC) OH OH Percent cyclohexylheptanol < 0.005 (HPLC) O HO pH (1% solution) 5-8 OH Solubility in water at 0-5°C ≥ 20% OH Conductance (10% solution) < 40 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.02 280 nm < 0.04 260 nm < 0.06 225 nm < 0.1 1. US Patent 5,674,987 and US Patent 5,763,586. 2. Anatrace measurement. 3. G. J. Babcock, M. Farzan, and J. Sodroski, J. Biol. Chem. 278, 3378-3385 (2003).
Notes: INDEX
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Catalog No. Product Name/Specifications Size Price
C327S CYMAL®-7, SOL-GRADE® 1 g 112.32 [7-Cyclohexyl-1-heptyl-β-D-maltoside1] 5 g 449.28 25 g 1797.12
FW 522.5 [349477-49-2] C25H46O11 2 CMC (H2O) ~ 0.19 mM (0.0099%) CH2OH CH2OH Purity ≥ 98% pure by HPLC analysis. O O O (CH2)7 Percent alpha < 10 (HPLC) OH OH Percent cyclohexylheptanol < 0.05 (HPLC) O HO pH (1% solution) 4-9 OH Solubility in water at 20°C ≥ 20% OH Conductance (10% solution) < 100 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.05 280 nm < 0.1 PRODUCT INFOMATION 260 nm < 0.1 225 nm < 0.2 See C327 for references.
C610 CYPFOS™-3, ANAGRADE® 1 g 135.51 [3-Cyclopentyl-1-propylphosphocholine] 5 g 542.06 25 g 2168.24
NEW PRODUCTS FW 293.3 [869548-41-4] C13H28NO4P 1 CMC (H2O) ~ 180 mM (5.3%) O
Purity ≥ 99% by HPLC analysis. + (CH ) O P O (CH ) N(CH ) pH (1% solution) 5-8 2 3 2 2 3 3 Solubility in water at 0-5°C ≥ 20% O Conductance (10% solution) < 100 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.02 280 nm < 0.08
PRODUCT LISTING 260 nm < 0.1 1. Anatrace measurement.
D365 n-DECYL-N,N-DIMETHYLAMINE-N-OXIDE, ANAGRADE® 1 g 38.23 5 g 152.92
FW 201.36 [2605-79-0] C12H27NO 25 g 611.69 1 CMC (H2O) ~ 10.48 mM (0.211%)
Purity ≥ 99% by HPLC analysis Purified to contain < 500 μm of equivalent peroxide. CH3 pH (1% solution) 4-9 Solubility in water at 20°C > 30% CH3(CH2)9 N O Conductance (10% solution) < 100 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 CH3 Absorbance of a 1% detergent solution: 340 nm < 0.02 280 nm < 0.06
INDEX 260 nm < 0.08 1. Anatrace measurement.
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Catalog No. Product Name/Specifications Size Price
D352 n-DECYL-N,N-DIMETHYLGLYCINE, ANAGRADE® 1 g 41.54 5 g 166.15
FW 243.4 [2644-45-3] C14H29O2N 25 g 664.60 1 CMC (H2O) ~ 19 mM (0.46%) Purity ≥ 99% by HPLC analysis. O CH3 PRODUCT INFORMATION _ + pH (1% solution) 5-8 O CCH2 N (CH2)9CH3 Solubility in water at 20°C ≥ 30% Conductance (10% solution) < 80 μS CH3 Absorbance of a 1% detergent solution: 340 nm < 0.05 280 nm < 0.08 260 nm < 0.1 1. Anatrace measurement.
D321 n-DECYL-β-D-GLUCOPYRANOSIDE, ANAGRADE® 1 g 56.18
[n-Decyl-β-D-glucoside] 5 g 224.72 NEW PRODUCTS 25 g 898.88
FW 320.4 [58846-77-8] C16H32O6 1 2 CMC (H2O) ~ 2.2 mM (0.070%) CMC (0.01M PO4 Buffer) ~ 2.3 mM Purity ≥ 99% by HPLC analysis. CH2OH For molar volume check reference 3. (CH ) CH Percent alpha < 2 (HPLC) O O 2 9 3 Percent decanol < 0.005 (HPLC) OH pH (0.03% solution) 5-8 HO Solubility in water at 20°C ≥ 0.1% OH Conductance (0.1% solution) < 20 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 0.1% detergent solution: 340 nm < 0.02 280 nm < 0.04 260 nm < 0.06 225 nm < 0.1 1. Helenius, A., McCaslin, D. R., Fries, E. and Tanford, C., Methods Enzymol. 56, 734-749 (1979). 2. Brito, R. M. M. and Vaz, W. L. C., Anal. Biochem. 152, 250-255 (1986). 3. Brown, G. M., Dubreuil, P., Ichhaporia, F. M. and Desnoyers, J. E., Canadian J. Chem. 48, 2525-2531 (1970).
Notes: INDEX
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Catalog No. Product Name/Specifications Size Price
D322 n-DECYL-β-D-MALTOPYRANOSIDE, ANAGRADE® 1 g 54.20 [n-Decyl-β-D-maltoside] 5 g 203.25 25 g 762.19
FW 482.6 [82494-09-5] C22H42O11 1 2 CMC (H2O) ~ 1.8 mM (0.087%) CMC (0.15M NaCl) ~ 1.8 mM 2 Aggregation Number (H2O) ~ 69
Purity ≥ 99% by HPLC analysis. CH2OH CH2OH For molar volume check reference 3. O O O (CH2)9CH3 Percent alpha < 2 (HPLC) OH OH Percent decanol < 0.005 (HPLC) HO O pH (1% solution) 5-8 Solubility in water at 0-5°C ≥ 20% OH OH Conductance (10% solution) < 40 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 PRODUCT INFOMATION Absorbance of a 1% detergent solution: 340 nm < 0.02 280 nm < 0.04 260 nm < 0.06 225 nm < 0.1 1. Alpes, H., Apell, H.-J., Knoll, G., Plattner, H. and Riek, R., Biochim. Biophys. Acta 946, 379-388 (1988). 2. Anatrace measurement in collaboration with Professor R. M. Garavito (Michigan State University). 3. Brown, G. M., Dubreuil, P., Ichhaporia, F. M. and Desnoyers, J. E., Canadian J. Chem. 48, 2525-2531 (1970). NEW PRODUCTS D322HA n-DECYL-α-D-MALTOPYRANOSIDE, ANAGRADE® 1 g 118.72 [n-Decyl-α-D-maltoside] 5 g 474.88 (Alpha isomer) 25 g 1899.52
FW 482.6 [168037-12-5] C22H42O11 CH2OH CH2OH Purity 99% by HPLC analysis. ≥ O O Percent alpha > 94 (HPLC) OH OH Percent dodecanol < 0.005 (HPLC) O pH (1% solution) 5-8 OH O (CH2)9CH3 OH OH PRODUCT LISTING Solubility in water at 0-5°C ≥ 20% Conductance (10% solution) < 40 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.05 280 nm < 0.10 260 nm < 0.15 225 nm < 0.25
Notes: INDEX
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Catalog No. Product Name/Specifications Size Price
D322LA n-DECYL-β-D-MALTOPYRANOSIDE, ANAGRADE® 1 g 80.53 [n-Decyl-β-D-maltoside] 5 g 322.10 (Low alpha) 25 g 1288.41
FW 482.6 [82494-09-5] C22H42O11 1 2 CMC (H2O) ~ 1.8 mM (0.087%) CMC (0.15M NaCl) ~ 1.8 mM PRODUCT INFORMATION 2 Aggregation Number (H2O) ~ 69 Purity ≥ 99% by HPLC analysis. CH2OH CH2OH For Molar volume check reference 3. O O O (CH2)9CH3 Percent alpha < 0.2 (HPLC) OH OH Percent decanol < 0.005 (HPLC) HO O pH (1% solution) 5-8 Solubility in water at 0-5°C ≥ 20% OH OH Conductance (10% solution) < 40 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.02 NEW PRODUCTS 280 nm < 0.04 260 nm < 0.06 225 nm < 0.1 See D322 for references.
D322S n-DECYL-β-D-MALTOPYRANOSIDE, SOL-GRADE® 1 g 45.09 [n-Decyl-β-D-maltoside] 5 g 169.10 25 g 634.14
FW 482.6 [82494-09-5] C22H42O11 1 2 CMC (H2O) ~ 1.8 mM (0.087%) CMC (0.15M NaCl) ~ 1.8 mM 2 Aggregation Number (H2O) ~ 69 Purity ≥ 97% pure by HPLC analysis. CH2OH CH2OH Percent alpha < 5 (HPLC) O O O (CH2)9CH3 Percent decanol < 0.05 (HPLC) OH OH pH (1% solution) 4-9 HO O Solubility in water at 20°C ≥ 20% Conductance (10% solution) < 100 μS OH OH Absorbance of a 1% detergent solution: 340 nm < 0.05 280 nm < 0.1 260 nm < 0.1 225 nm < 0.2 See D322 for references.
Notes: INDEX
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Catalog No. Product Name/Specifications Size Price
D323 n-DECYL-β-D-THIOGLUCOPYRANOSIDE, ANAGRADE® 1 g 82.13 [n-Decyl-β-D-thioglucoside] 5 g 307.99 25 g 1154.94
FW 336.4 [98854-16-1] C16H32O5S 1 CMC (methanol/water) ~ 0.9 mM (0.30%) CH2OH O Purity ≥ 99% by HPLC analysis. S (CH2)9CH3 Percent alpha < 2 (HPLC) OH pH (1% solution2) 5-8 HO 2 Solubility in methanol:water at 20°C ≥ 1% OH Conductance (1% solution2) < 40 μS Percent fluorescence due to a 0.1% detergent solution2 at 345 nm < 10 Absorbance of a 1% detergent solution2: 340 nm < 0.05 280 nm < 0.15 260 nm < 0.2 PRODUCT INFOMATION NOTE: n-Decyl-β-D-thioglucopyranoside is insoluble in water. 1. Anatrace measurement. 2. Solvent: (1:1) v/v methanol : water.
D335 n-DECYL-β-D-THIOMALTOPYRANOSIDE, ANAGRADE® 1 g 82.13 [n-Decyl-β-D-thiomaltoside] 5 g 307.99 25 g 1154.94 FW 498.6 [14565-56-4] C H O S
NEW PRODUCTS 22 42 10 1 CMC (H2O) ~ 0.9 mM (0.045%) CH2OH CH2OH
O O S (CH2)9CH3 Purity ≥ 99% by HPLC analysis. OH OH Percent alpha < 2 (HPLC) HO O pH (1% solution) 5-8 OH Solubility in water at 20°C ≥ 20% OH Conductance (10% solution) < 40 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10
PRODUCT LISTING Absorbance of a 1% detergent solution: 340 nm < 0.05 280 nm < 0.15 260 nm < 0.2 1. Anatrace measurement. 2. Mechref, Y. and Rassi, Z. E., J. Chromatography 757, 263-273 (1997).
Notes: INDEX
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Catalog No. Product Name/Specifications Size Price
D354 DECYLTRIMETHYLAMMONIUM CHLORIDE 5 g 20.28 25 g 81.12 FW 236.0 [10108-87-9] C H ClN 100 g 259.58 13 30 CH CMC (H O) ~ 2.95 mM1 (0.07%) 3 2 + Purity ≥ 95% pure by titration. CH3 N (CH2)9CH3
pH (1% solution) 5.5-8.5 PRODUCT INFORMATION Solubility in water at 20°C ≥ 30% - CH3 Cl Absorbance of a 1% detergent solution: 340 nm < 0.02 280 nm < 0.04 260 nm < 0.06 225 nm < 0.08 1. Anatrace measurement. 2. Neugebauer, J. M., Methods Enzymol. 182, 239-253 (1990). 3. Maillet, J.-B., Lachet, V., and Coveney, P. V., Phys. Chem. Chem. Phys. 1, 5277-5290 (1999).
D380 DEOXYCHOLIC ACID, SODIUM SALT, ANAGRADE® 5 g 49.00
[3α,12α-Dihydroxy-5β-cholan-24-oic acid, monosodium salt] 25 g 196.00 NEW PRODUCTS 100 g 627.20
FW 414.6 [302-95-4] C24H39O4Na 1 CMC (H2O) ~ 6 mM (0.24%) 2 H3C Aggregation Number (H2O) ~ 22 OH CH _ 3 + Purity ≥ 98% pure by HPLC analysis. COO Na
pH (1% solution) 5-9 H CH Solubility in water at 20° C ≥ 5% 3 Absorbance of a 1% detergent solution 340 nm < 0.02 H 280 nm < 0.04 HO 260 nm < 0.06 H
1. Anatrace measurement. 2. Black, Shaun D.: http://psyche.uthct.edu/shaun/SBlack/detergnt.html
D412 DIFOS™-12, ANAGRADE® 250 mg Please Inquire [Dodecyl-1,2-diphosphocholine] 1 g 5 g FW 532.6 [869584-01-0] C H N O P 22 50 2 8 2 O H 1 CMC (H2O) 1.18 mM (0.063%) + (CH ) N (CH ) O P O CH C (CH ) CH Purity ≥ 99% by HPLC analysis. 3 3 2 2 2 2 9 3 pH (1% solution) 5-8 _ O O o Solubility in water at 0-5 C ≥ 20% _ Conductance (10% solution) < 100 μS O P O Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 O Absorbance of a 1% detergent solution: 340 nm < 0.05
280 nm < 0.1 (CH2 )2 260 nm < 0.2 + 1. Anatrace measurement. N(CH3 )3 INDEX
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Catalog No. Product Name/Specifications Size Price
DH325 2,6-DIMETHYL-4-HEPTYL-β-D-MALTOPYRANOSIDE, ANAGRADE® 1 g 76.96 5 g 307.84 25 g 1231.36
FW 468.5 [869638-31-3] C21H40O11 1 CH3 CMC (H2O) ~ 27.5 mM (1.2%) CH CHCH Purity ≥ 99% by HPLC analysis. CH2 OH CH2 OH 2 3 Percent alpha < 15% O O O CH OH OH Percent (2-6-dimethyl-4-heptanol) < 0.005% CH2 CHCH 3 pH (1% solution) 5-8 HO O CH3 Solubility in water at 0-5oC ≥ 20% OH OH Conductance (10% solution) < 40 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.02 280 nm < 0.04
PRODUCT INFOMATION 260 nm < 0.06 225 nm < 0.1 1. Anatrace measurement.
D330 DIMETHYLDECYLPHOSPHINE OXIDE, ANAGRADE® 1 g 39.00 [Decyldimethyl-phosphine oxide] 5 g 156.00 25 g 624.00
FW 218.3 [2190-95-6] C12H27OP
NEW PRODUCTS 1 CMC (H2O) ~ 4.66 mM (0.10%) Aggregation Number (H O)1 ~ 31 2 CH3 Purity ≥ 99% pure by HPLC analysis. pH (1% solution) 5-8 CH3(CH2)9 P O Solubility in water at 0-5oC ≥ 20% Conductance (10% solution) < 100 μS CH Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 3 Absorbance of a 1% detergent solution: 340 nm < 0.05 280 nm < 0.08
PRODUCT LISTING 260 nm < 0.1 1. Herrmann, K. W., Brushmiller, J. G. and Courchene, W. L., Micellar Properties of Dimethlalkylphosphine Oxide Solutions 70, Number 9, 2909 (1966).
D328 DIMETHYLOCTYLPHOSPHINE OXIDE, ANAGRADE® 1 g 39.00 [Octyl dimethyl-phosphine oxide] 5 g 156.00 25 g 624.00
FW 190.1 [13232-68-3] C10H23OP 1 CMC (H2O) ~ 40 mM (0.76%) Aggregation Number (H O)1 ~ 41 2 CH3 Purity ≥ 99% pure by HPLC analysis. o Solubility in water at 0-5 C ≥ 20% CH3(CH2)7 P O Conductance (10% solution) < 100 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 CH Absorbance of a 1% detergent solution: 340 nm < 0.05 3 280 nm < 0.08 INDEX 260 nm < 0.1 1. Herrmann, K. W., Brushmiller, J. G. and Courchene, W. L., Micellar Properties of Dimethlalkylphosphine Oxide Solutions 70, Number 9, 2909 (1966). 48 800-252-1280 • 419-891-3030 • FAX 419-891-3037 • www.anatrace.com Product Listing SERVICE INFORMATION
Catalog No. Product Name/Specifications Size Price
D360 n-DODECYL-N,N-DIMETHYLAMINE-N-OXIDE, ANAGRADE® 1 g 30.97 [Lauryldimethylamine-N-oxide / LDAO / DDAO / 5 g 123.86 N,N-Dimethyl-1-dodecanamine-N-oxide] 25 g 495.47
FW 229.41 [1643-20-5] C14H31NO 5 5
CMC (H O) ~ 1-2 mM (0.023%) CMC (0.1M NaCl) ~ 0.14 mM PRODUCT INFORMATION 2 CH3 6 Aggregation Number (H2O) ~ 76
Purity ≥ 99% by HPLC analysis. CH3(CH2)11 N O Purified to contain < 500 μM of equivalent peroxide. pH (1% solution) 6-8.5 CH3 Solubility in water at 20°C ≥ 30% Conductance (10% solution) < 100 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.02 280 nm < 0.06 260 nm < 0.08 NEW PRODUCTS 1. Michel, H., J. Mol. Biol. 158, 567-572 (1982). 2. Malkin, R., Arch. Biochem. Biophys. 169, 77-83 (1975). 3. Reithmeier, A. F., et al, Biochem. 32, 1172-1179 (1993). 4. Dawkins, D. J., et al, in Crystallization of Membrane Proteins (Hartmut Michel, Ed.) 125-137, CRC Press, Boca Raton (1991). 5. Herrmann, K. W., J. Phys. Chem. 66, 292 (1962). 6. Herrmann, K. W. J., Colloid Interface Sci. 22, 352 (1966).
D360S n-DODECYL-N,N-DIMETHYLAMINE-N-OXIDE, SOL-GRADE® 1 g 24.77 [Lauryldimethylamine-N-oxide / LDAO / DDAO / 5 g 99.11 N,N-Dimethyl-1-dodecanamine-N-oxide] 25 g 396.43
FW 229.41 [1643-20-5] C14H31NO 5 5 CMC (H2O) ~ 1-2 mM (0.023%) CMC (0.1M NaCl) ~ 0.14 mM
Purity ≥ 95% pure by HPLC analysis. CH3 Purified to contain < 500 μM of equivalent peroxide. pH (1% solution) 5-9 CH3(CH2)11 N O Solubility in water at 20°C ≥ 30% Conductance (10% solution) < 500 μS CH Absorbance of a 1% detergent solution: 340 nm < 0.1 3 280 nm < 0.1 260 nm < 0.2 Note: This material contains lower organic peroxide levels than the commercial grade, but higher levels than our ANAGRADE® detergent. The amount of equivalent peroxide is listed on the Certificate of Analysis for each lot. See D360 for references. INDEX
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Catalog No. Product Name/Specifications Size Price
D350 n-DODECYL-N,N-DIMETHYLGLYCINE, ANAGRADE® 1 g 41.54 (Major component of the industrial detergent, Empigen BB®) 5 g 166.15 25 g 664.60
FW 271.4 [683-10-3] C16H33O2N CMC (H O) ~ 1.5 mM1 (0.041%) 2 O CH3 Purity ≥ 99% by HPLC analysis. _ + pH (1% solution) 5-8 O CCH2 N (CH2)11 CH3 Solubility in water at 20°C ≥ 30% Conductance (10% solution) < 80 μS CH3 Absorbance of a 1% detergent solution: 340 nm < 0.05 280 nm < 0.08 260 nm < 0.1 1. Personal communication from Professor R. M. Garavito (Michigan State University). PRODUCT INFOMATION D350S n-DODECYL-N,N-DIMETHYLGLYCINE, SOL-GRADE® 1 g 31.35 (Major component of the industrial detergent, Empigen BB®) 5 g 125.38 25 g 501.52
FW 271.4 [683-10-3] C16H33O2N CMC (H O) ~ 1.5 mM1 (0.041%) 2 O CH3 Purity ≥ 98% pure by HPLC analysis. _ + pH (1% solution) 4-9 O CCH2 N (CH2)11 CH3 NEW PRODUCTS Solubility in water at 20oC ≥ 30% Conductance (10% solution) < 500 μS CH3 Absorbance of a 1% detergent solution: 340 nm < 0.2 280 nm < 0.4 260 nm < 0.8 1. Personal communication from Professor R. M. Garavito (Michigan State University).
D318 n-DODECYL-β-D-GLUCOPYRANOSIDE, ANAGRADE® 1 g 58.30 [n-Dodecyl-β-D-glucoside] 5 g 222.60 PRODUCT LISTING 25 g 834.74
FW 348.5 [59122-55-3] C18H36O6 1 CMC (H2O) ~ 0.19 mM (0.0066%) CH2OH Purity ≥ 99% by HPLC analysis. O (CH ) CH For molar volume check reference 2. O 2 11 3 Percent alpha < 2 (HPLC) OH Percent dodecanol < 0.005 (HPLC) HO pH (0.005% solution) 5-8 OH Solubility in water at 20°C ≥ 0.008% Conductance (0.005% solution) < 10 μS Percent fluorescence due to a 0.005% detergent solution at 345 nm < 10 Absorbance of a 0.005% detergent solution: 340 nm < 0.06 280 nm < 0.06 260 nm < 0.06 225 nm < 0.2 1. Helenius, A., McCaslin, D. R., Fries, E. and Tanford, C., Methods Enzymol. 56, 734-749 (1979). INDEX 2. Brown, G. M., Dubreuil, P., Ichhaporia, F. M. and Desnoyers, J. E., Canadian J. Chem. 48, 2525-2531 (1970).
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Catalog No. Product Name/Specifications Size Price
D345 n-DODECYL-β-IMINODIPROPIONIC ACID, DISODIUM SALT 5 g 25.79 (Disodium-N-lauryl-β-iminodipropionate) 25 g 103.17 100 g 330.14 O FW 373.4 [3655-00-3] C18H33NO4Na2 - + Purity ≥ 97% pure by HPLC analysis. CH3 (CH2)11 N (CH2)2 C O Na pH (0.5% solution) 9-12 PRODUCT INFORMATION (CH2)2 Solubility in water at 0-5°C ≥ 20% Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 C O - Absorbance of a 0.5% detergent solution 340 nm < 0.05 O 280 nm < 0.1 + 260 nm < 0.3 Na NOTE: Heating may be required to dissolve detergent.
D346 n-DODECYL-β-IMINODIPROPIONIC ACID, MONOSODIUM SALT 5 g 25.79 (Monosodium-N-lauryl-β-iminodipropionate) 25 g 103.17 100 g 330.14 NEW PRODUCTS
FW 351.5 [14960-06-6] C18H34NO4Na O Purity 97% pure by HPLC analysis. - + ≥ CH3 (CH2)11 N (CH2)2 C O Na pH (0.5% solution) 6.0-8.5 Solubility in water at 0-5°C ≥ 20% (CH2)2 Off white in color C O Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 0.5% detergent solution 340 nm < 0.05 OH 280 nm < 0.1 260 nm < 0.3 NOTE: Heating may be required to dissolve detergent.
D310HA n-DODECYL-α-D-MALTOPYRANOSIDE, ANAGRADE® 1 g 118.72 [n-Dodecyl-α-D-maltoside / Lauryl maltoside] 5 g 474.88 (Alpha isomer) 25 g 1899.52
FW 510.6 [116183-64-3] C24H46O11 CMC (H O) ~ 0.15 mM1-2 (0.0076%) 2 CH2OH CH2OH Aggregation Number (H O)2 ~ 90 2 O O Purity ≥ 99% by HPLC analysis. OH Percent alpha > 94 (HPLC) OH HO O Percent dodecanol < 0.005 (HPLC) O (CH2)11 CH3 pH (1% solution) 5-8 OH OH Solubility in water at 0-5°C ≥ 20% Conductance (10% solution) < 40 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.05 280 nm < 0.10 260 nm < 0.15 INDEX 225 nm < 0.25 1. Anatrace measurement. 2. VanAken, T., Foxall-VanAken, S., Castleman, S. and Ferguson-Miller, S., Methods Enzymol. 125, 27-35 (1986).
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Catalog No. Product Name/Specifications Size Price
D310 n-DODECYL-β-D-MALTOPYRANOSIDE, ANAGRADE® 1 g 63.00 [n-Dodecyl-β-D-maltoside / Lauryl maltoside / 5 g 236.25 Dodecyl 4-O-α-D-glucopyranosyl-β-D-glucopyranoside] 25 g 885.94
FW 510.6 [69227-93-6] C24H46O11 1 2 CMC (H2O) ~ 0.17 mM (0.0087%) CMC (0.2 M NaCl) ~ 0.12 mM 1-2 Aggregation Number (H2O) ~ 78-149 Purity ≥ 99% by HPLC analysis. CH2OH CH2OH For molar volume check reference 3. O O O (CH2)11 CH3 Percent alpha < 2 (HPLC) OH OH Percent dodecanol < 0.005 (HPLC) HO O pH (1% solution) 5-8 Solubility in water at 0-5°C ≥ 20% OH OH Conductance (10% solution) < 40 μS PRODUCT INFOMATION Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.02 280 nm < 0.04 260 nm < 0.06 225 nm < 0.1 1. VanAken, T., Foxall-VanAken, S., Castleman, S. and Ferguson-Miller, S., Methods Enzymol. 125, 27-35 (1986). 2. Anatrace measurement in collaboration with Professor R. M. Garavito (Michigan State University). 3. Brown, G. M., Dubreuil, P., Ichhaporia, F. M. and Desnoyers, J. E., Canadian J. Chem. 48, 2525-2531 (1970). NEW PRODUCTS
D310A n-DODECYL-β-D-MALTOPYRANOSIDE, ANAGRADE® 1 g 56.70 [n-Dodecyl-β-D-maltoside / Lauryl maltoside / 5 g 212.63 Dodecyl 4-O-α-D-glucopyranosyl-β-D-glucopyranoside] 25 g 797.34 (Contains up to 15% alpha isomer)
FW 510.6 [69227-93-6] C24H46O11 1 2 CMC (H2O) ~ 0.17 mM (0.0087%) CMC (0.2 M NaCl) ~ 0.12 mM 1-2 Aggregation Number (H2O) ~ 78-149 CH2OH CH2OH PRODUCT LISTING Purity ≥ 99% by HPLC analysis. Percent alpha < 15 (HPLC) O O O (CH2)11 CH3 Percent dodecanol < 0.005 (HPLC) OH OH pH (1% solution) 5-8 HO O Solubility in water at 0-5°C ≥ 20% OH OH Conductance (10% solution) < 40 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.02 280 nm < 0.04 260 nm < 0.06 225 nm < 0.1 See D310 for references. INDEX
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Catalog No. Product Name/Specifications Size Price
D310LA n-DODECYL-β-D-MALTOPYRANOSIDE, ANAGRADE® 1 g 87.00 [n-Dodecyl-β-D-maltoside / Lauryl maltoside / 5 g 326.25 Dodecyl 4-O-α-D-glucopyranosyl-β-D-glucopyranoside] 25 g 1223.44 (Low alpha)
FW 510.6 [69227-93-6] C24H46O11 1 2 PRODUCT INFORMATION CMC (H2O) ~ 0.17 mM (0.0087%) CMC (0.2 M NaCl) ~ 0.12 mM 2 Aggregation Number (H2O) ~ 78-149 CH2OH CH2OH Purity ≥ 99% by HPLC analysis. O O (CH ) CH Percent alpha < 0.2 (HPLC) O 2 11 3 Percent dodecanol < 0.005 (HPLC) OH OH pH (1% solution) 5-8 HO O Solubility in water at 0-5°C ≥ 20% OH OH Conductance (10% solution) < 40 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.02
280 nm < 0.04 NEW PRODUCTS 260 nm < 0.06 225 nm < 0.1 See D310 for references.
D310S n-DODECYL-β-D-MALTOPYRANOSIDE, SOL-GRADE® 1 g 50.50 [n-Dodecyl-β-D-maltoside / Lauryl maltoside / 5 g 189.00 Dodecyl 4-O-α-D-glucopyranosyl-β-D-glucopyranoside] 25 g 708.75
FW 510.6 [69227-93-6] C24H46O11 1 2 CMC (H2O) ~ 0.17 mM (0.0087%) CMC (0.2 M NaCl) ~ 0.12 mM 2 Aggregation Number (H2O) ~ 78-149 CH2OH CH2OH Purity ≥ 98% pure by HPLC analysis. O O O (CH2)11 CH3 Percent alpha < 5 (HPLC) Percent dodecanol < 0.05 (HPLC) OH OH O pH (1% solution) 4-9 HO Solubility in water at 20°C ≥ 20% OH OH Conductance (10% solution) < 100 μS Absorbance of a 1% detergent solution: 340 nm < 0.05 280 nm < 0.1 260 nm < 0.1 225 nm < 0.2 See D310 for references. INDEX
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Catalog No. Product Name/Specifications Size Price
D310T n-DODECYL-d25-β-D-MALTOPYRANOSIDE 100 mg 407.04
(n-Dodecyl-d25-β-D-maltoside, Lauryl maltoside) 250 mg 915.84 500 mg 1740.10
FW 535.8 [849110-74-3] C24D25H21O11 1 CMC (H2O) ~ 0.2 mM CH2OH CH2OH Purity ≥ 97% pure by HPLC analysis. O O O (CD2)11 CD3 Percent alpha < 15 (HPLC) OH OH Percent dodecanol < 0.05 (HPLC) HO O pH (1% solution) 5-8 Solubility in water at 20°C ≥ 10% OH OH Conductance (1% solution) < 200 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.1 280 nm < 0.25 PRODUCT INFOMATION 260 nm < 0.25 225 nm < 0.8 1. CMC value for the undeuterated compound.
D342 n-DODECYL-β-D-THIOMALTOPYRANOSIDE, ANAGRADE® 1 g 82.13 (n-Dodecyl-β-D-thiomaltoside) 5 g 307.99 25 g 1154.94 NEW PRODUCTS FW 526.6 [148565-58-6] C24H46O10S 1 CH2OH CH2OH CMC (H2O) ~ 0.05 mM (0.0026%) O O S (CH ) CH Purity ≥ 98% pure by HPLC analysis. 2 11 3 Percent alpha < 2 (HPLC) OH OH pH (1% solution) 5-8 HO O Solubility in water at 20°C ≥ 10% OH OH Conductance (10% solution) < 40 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.05 PRODUCT LISTING 280 nm < 0.15 260 nm < 0.2 1. Anatrace measurement. 2. Mechref, Y. and Rassi, Z. E., J. Chromatography 757, 263-273 (1997). INDEX
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Catalog No. Product Name/Specifications Size Price
D355 DODECYLTRIMETHYLAMMONIUM CHLORIDE 5 g 20.28 25 g 81.12
FW 263.89 [112-00-5] C15H34ClN 100 g 259.58 1 CH3 CMC (H2O) ~ 0.046 mM (0.0012%) 4,5 + Aggregation Number (H2O) ~ 44-61 CH3 N (CH2)11 CH3
Purity ≥ 95% pure by titration. PRODUCT INFORMATION - CH3 pH (1% solution) 5.5-8.5 Cl Solubility in water at 20°C ≥ 30% Absorbance of a 1% detergent solution: 340 nm < 0.02 280 nm < 0.04 260 nm < 0.06 225 nm < 0.08 Note: Measured as the bromide salt. 1. Anatrace measurement. 2. J. Mata, J. Patel, N. Jain, et al, J. Colloid Interface Sci. Dec. 22, (2005). 3. H. Matsubara, T. Nakano, T. Matsuda, et al, Langmuir 21, 8131-8137 (2005). 4. Neugebauer, J. M., Methods Enzymol. 182, 239-253 (1990). 5. Maillet, J.-B., Lachet, V., and Coveney, P. V., Phys. Chem. Chem. Phys. 1, 5277-5290 (1999). NEW PRODUCTS
D2001 DTE 1 g 11.00 [Dithioerythritol] 5 g 46.00 25 g 185.00
FW 154.25 [6892-68-8] C4H10O2S2 Form: White crystalline powder. OH
Assay (I2): ≥ 99% SH HS Chromatography (TLC [(CH2Cl2:Et2O/2:1)]): Homogeneous Melting Point:≥ 81°C OH
Absorance: (283 nm, 0.02 M, H2O): ≥ 0.04
D1000 DTNB 5 g 43.00 [5,5'-Dithiobis-(2-nitrobenzoic) acid] 25 g 177.00
O FW 396.36 [69-78-3] C14H6N2O8S2 Form: Light yellow crystalline powder N+ O- Assay (Titration with 0.1N NaOH in ethanol): ≥ 99.0% S OH Melting Range: 236-248° C S -O O N+ O OOH
D2000 DTT, CLELAND'S REAGENT 5 g 19.76 [1,4-Dithiothreitol] 25 g 79.04 100 g 269.36
FW 154.2 [27565-41-9] C4H10O2S2 OH Purity (SH) Iodometric titration > 97% SH INDEX Solubility in water at 20oC: 3% Melting Point: 41-43oC HS Oxidized dithiothreitol < 0.5% OH Appearance: White Powder
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Catalog No. Product Name/Specifications Size Price
D2000A DTT, CLELAND'S REAGENT, ANAGRADE® 1 g 16.64 [1,4-Dithiothreitol] 5 g 66.56 25 g 266.24
FW 154.24 [27565-41-9] C4H10O2S2 Purity (SH) Iodometric titration ≥ 99.5% Absorbance: (280 nm, 0.1M, 1cm): < 0.08 OH Absorbance: (260 nm, 0.1M, 1cm): < 0.40 SH Melting Point: 40-43oC Oxidized DTT: < 0.3% HS DNase: None detected OH RNase: None detected Protease: None detected Storage: -20oC Appearance: White Crystalline Powder PRODUCT INFOMATION
E1000 EDTA 100 g 17.00 [Ethylene diaminetetracetic acid, Disodium salt, Dihydrate] 500 g 46.00
. FW 372.24 [6381-92-6] C10H14N2Na2O8 2H2O Form: White crystalline powder Assay (Titration): 99.0 to 101.0% O O NEW PRODUCTS Absorbance: (280 nm, 0.1M, 1cm): < 0.05 OH Absorbance: (260 nm, 0.1M, 1cm): < 0.2 NaO N Insoluble matter: < 0.005% 2H2O pH (5%, H2O): 4.0 to 6.0 N ONa Nitrilotriacetic Acid: < 0.1% HO Heavy Metals (Pb): < 0.005% O O Iron (Fe): < 0.01% Arsenic (As): < 1 ppm Calcium (Ca): Passes test
PRODUCT LISTING Solubility (0.1M, H2O): Complete clear and colorless DNase (endo): None detected DNase (exo): None detected RNase: None detected
NOTES: INDEX
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Catalog No. Product Name/Specifications Size Price
E1001 EGTA 10 g 66.00 [Ethylene glycol-O,O'-bis(2-aminoethyl)-N,N,N',N'-tetracetic acid] 50 g 303.00
FW 380.35 [67-42-5] C14H24N2O10 Assay (Titration): ≥ 99.0% OH
Absorbance (280 nm, 0.1M in 1N NaOH, 1cm): < 0.10 PRODUCT INFORMATION O O Loss on Drying: < 0.5% N Cadmium (Cd): < 5 ppm O OH Chloride (Cl): < 0.005% O O Cobalt (Co): < 5 ppm N Copper (Cu): < 5 ppm HO OH Iron (Fe): < 5 ppm Lead (Pb): < 5 ppm O Nickel (Ni): < 5 ppm
Sulfate (SO4): < 0.02% Zinc (Zn): < 5 ppm NEW PRODUCTS
E2000 NEM 5 g 36.00 [N-Ethylmaleimide] 10 g 59.00 25 g 121.00
FW 125.13 [128-53-0] C6H7NO2 Form: White crystalline powder O N O Melting Point: 44-45°C Storage: +4°C
F300 FOS-CHOLINE®-8, ANAGRADE® 1 g 141.78 [n-Octylphosphocholine] 5 g 567.12 25 g 2270.11
FW 295.4 [53255-89-3] C13H30NO4P 1 CMC (H2O) ~ 114 mM (3.4%) O + Purity 99% by HPLC analysis. ≥ CH3(CH2)7 O P O (CH2)2 N(CH3)3 pH (1% solution) 5-8 _ Solubility in water at 0-5°C ≥ 20% O Conductance (10% solution) < 200 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.02 280 nm < 0.08 260 nm < 0.1 1. Anatrace measurement. 2. O. Vinogradova, F. Sonnichsen, C. R. Sanders, J. Biomol. NMR 11, 381-386 (1998). INDEX
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Catalog No. Product Name/Specifications Size Price
F300S FOS-CHOLINE®-8, SOL-GRADE® 1 g 113.22 [n-Octylphosphocholine] 5 g 452.88 25 g 1811.52
FW 295.4 [53255-89-3] C13H30NO4P 1 CMC (H2O) ~ 114 mM (3.4%) O + Purity 97% by HPLC analysis. ≥ CH3(CH2)7 O P O (CH2)2 N(CH3)3 pH (1% solution) 4-9 _ 10% solution may appear slightly hazy or cloudy at room temperature. O Conductance (10% solution) < 500 μS Absorbance of a 1% detergent solution: 340 nm < 0.1 280 nm < 0.2 260 nm < 0.2 See F300 for references. PRODUCT INFOMATION F302 FOS-CHOLINE®-9, ANAGRADE® 1 g 141.78 [n-Nonylphosphocholine] 5 g 567.12 25 g 2270.11
FW 309.4 [253678-64-7] C14H32NO4P CMC (H O) ~ 39.5 mM1 (1.2%) 2 O + Purity ≥ 99% by HPLC analysis. CH3(CH2)8 O P O (CH2)2 N(CH3)3 pH (1% solution) 5-8 _ NEW PRODUCTS Solubility in water at 0-5°C ≥ 20% O Conductance (10% solution) < 200 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.02 280 nm < 0.08 260 nm < 0.1 1. Anatrace measurement.
F302S FOS-CHOLINE®-9, SOL-GRADE® 1 g 113.22
PRODUCT LISTING [n-Nonylphosphocholine] 5 g 452.88 25 g 1811.52
FW 309.4 [253678-64-7] C14H32NO4P CMC (H O) ~ 39.5 mM1 (1.2%) 2 O + Purity ≥ 97% by HPLC analysis. CH3(CH2)8 O P O (CH2)2 N(CH3)3 pH (1% solution) 4-9 _ 10% solution may appear slightly hazy or cloudy at room temperature. O Conductance (10% solution) < 500 μS Absorbance of a 1% detergent solution: 340 nm < 0.1 280 nm < 0.2 260 nm < 0.2 1. Anatrace measurement. INDEX
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Catalog No. Product Name/Specifications Size Price
F304 FOS-CHOLINE®-10, ANAGRADE® 1 g 126.48 [n-Decylphosphocholine] 5 g 505.92 25 g 2023.68
FW 323.4 [70504-28-8] C15H34NO4P CMC (H O) ~ 11 mM1 (0.35%) 2 O
+ PRODUCT INFORMATION Purity ≥ 99% by HPLC analysis. CH3(CH2)9 O P O (CH2)2 N(CH3)3 pH (1% solution) 5-8 _ Solubility in water at 0-5°C ≥ 20% O Conductance (10% solution) < 200 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.02 280 nm < 0.08 260 nm < 0.1 1. Anatrace measurement. 2. Cortes, D. M. and Perozo, E., Biochem. 36, 10343-10352 (1997). NEW PRODUCTS F304S FOS-CHOLINE®-10, SOL-GRADE® 1 g 100.98 [n-Decylphosphocholine] 5 g 403.92 25 g 1615.68
FW 323.4 [70504-28-8] C15H34NO4P CMC (H O) ~ 11 mM1 (0.35%) O 2 + Purity ≥ 97% pure by HPLC analysis. CH3(CH2)9 O P O (CH2 )2 N(CH3)3 pH (1% solution) 4-9 _ O 10% solution may appear slightly hazy or cloudy at room temperature. Conductance (10% solution) < 500 μS Absorbance of a 1% detergent solution: 340 nm < 0.1 280 nm < 0.2 260 nm < 0.2 See F304 for references.
F306 FOS-CHOLINE®-11, ANAGRADE® 1 g 141.68 [n-Undecylphosphocholine] 5 g 567.12 25 g 2270.11
FW 337.4 [253678-65-8] C16H36NO4P CMC (H O) ~ 1.85 mM1 (0.062%) 2 O + Purity ≥ 99% by HPLC analysis. CH3(CH2 )10 O P O (CH2 )2 N(CH3)3 pH (1% solution) 5-8 _ Solubility in water at 0-5oC ≥ 20% O Conductance (10% solution) < 200 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.02 280 nm < 0.08 260 nm < 0.1 1. Anatrace measurement. INDEX
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Catalog No. Product Name/Specifications Size Price
F306S FOS-CHOLINE®-11, SOL-GRADE® 1 g 113.22 [n-Undecylphosphocholine] 5 g 452.88 25 g 1811.52
FW 337.4 [253678-65-8] C16H36NO4P CMC (H O) ~ 1.85 mM1 (0.062%) 2 O + Purity ≥ 97% pure by HPLC analysis. CH3(CH2)10 O P O (CH2 )2 N(CH3)3 pH (1% solution) 4-9 _ 10% solution may appear slightly hazy or cloudy at room temperature. O Conductance (10% solution) < 500 μS Absorbance of a 1% detergent solution: 340 nm < 0.1 280 nm < 0.2 260 nm < 0.2 1. Anatrace measurement. PRODUCT INFOMATION F308 FOS-CHOLINE®-12, ANAGRADE® 1 g 126.48 [n-Dodecylphosphocholine] 5 g 505.92 25 g 2023.68
FW 351.5 [29557-51-5] C15H38NO4P 1 CMC (H2O) ~ 1.5 mM (0.047%) Aggregation Number (H O)6 ~ 50-60 2 O Purity ≥ 99% by HPLC analysis. + CH3 (CH 2 )11 O P O (CH 2 )2 N(CH 3 )3 NEW PRODUCTS pH (1% solution) 5-8 _ Solubility in water at 0-5°C ≥ 20% O Conductance (10% solution) < 200 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.02 280 nm < 0.08 260 nm < 0.1 1. Anatrace measurement. 2. C. Fares, D. S. Libich, G. Harauz, FEBS J. 273, 601-614 (2006). 3. R. Brunecky, S. Lee, P. W. Rzepecki, et al, Biochemistry 44, 16064-16071 (2005).
PRODUCT LISTING 4. K. Oxenoid, J. J. Chou, Proc. Natl. Acad. Sci. USA 102, 10870-10875 (2005). 5. Uteng, M., Hauge, H. H., Markwick, P. R. L., Fimland, G., Mantzilas, D., Nissen-Meyer, J., and Muhle-Goll, C., Biochem. 42, 11417-11426 (2003). 6. LeMaire, M., Champeil, P. and Moller, J. V., Biochimica et Biophysica Acta 1508, 86-111 (2000).
F308D FOS-CHOLINE®-12, DEUTERATED 100 mg 202.98
[n-Dodecylphosphocholine (D38)] 500 mg 774.18 1 g 1417.80 FW 389.79 [130890-78-7] C D NO P 17 38 4 O 1 CMC (H2O) ~ 1.5 mM (0.047%) + CD3(CD2)11 O P O (CD2)2 N(CD3)3 Purity ≥ 90% pure by HPLC analysis. _ pH (1% solution) 4-9 O 1. CMC value for the undeuterated compound. 2. See F308 for references. INDEX
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Catalog No. Product Name/Specifications Size Price
F308S FOS-CHOLINE®-12, SOL-GRADE® 1 g 100.98 [n-Dodecylphosphocholine] 5 g 403.92 25 g 1615.68
FW 351.5 [29557-51-5] C17H38NO4P CMC (H O) ~ 1.5 mM1 (0.047%) 2 O
+ PRODUCT INFORMATION Purity ≥ 97% pure by HPLC analysis. CH3(CH2)11 O P O (CH2 )2 N(CH3)3 pH (1% solution) 4-9 _ 10% solution may appear slightly hazy or cloudy at room temperature. O Conductance (10% solution) < 500 μS Absorbance of a 1% detergent solution: 340 nm < 0.1 280 nm < 0.2 260 nm < 0.2 1. See F308 for references.
F310 FOS-CHOLINE®-13, ANAGRADE® 1 g 141.78
[n-Tridecylphosphocholine] 5 g 567.12 NEW PRODUCTS 25 g 2270.11
FW 365.5 [85775-42-4] C18H40NO4P CMC (H O) ~ 0.75 mM1 (0.027%) 2 O + Purity ≥ 99% by HPLC analysis. CH3(CH2)12 O P O (CH2 )2 N(CH3)3 pH (1% solution) 5-8 _ Solubility in water at 0-5°C ≥ 20% O Conductance (10% solution) < 200 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.02 280 nm < 0.08 260 nm < 0.1 1. Anatrace measurement.
F310S FOS-CHOLINE®-13, SOL-GRADE® 1 g 113.22 [n-Tridecylphosphocholine] 5 g 452.88 25 g 1811.52
FW 365.5 [85775-42-4] C18H40NO4P 1 CMC (H2O) ~ 0.75 mM (0.027%) O + Purity ≥ 97% by HPLC analysis. CH3(CH2)12 O P O (CH2 )2 N(CH3)3 pH (1% solution) 4-9 _ 10% solution may appear slightly hazy or cloudy at room temperature. O Conductance (10% solution) < 500 μS Absorbance of a 1% detergent solution: 340 nm < 0.1 280 nm < 0.2 260 nm < 0.2 1. Anatrace measurement. INDEX You can search for a detergent by catalog number (pgs. 119-120), chemical name (pgs. 121-125), or CAS registry number (pgs. 126-129). A search program is also available at www.anatrace.com.
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Catalog No. Product Name/Specifications Size Price
F312 FOS-CHOLINE®-14, ANAGRADE® 1 g 141.78 [n-Tetradecylphosphocholine] 5 g 567.12 25 g 2270.11
FW 379.5 [77733-28-9] C19H42NO4P CMC (H O) ~ 0.12 mM1 (0.0046%) 2 O + Purity ≥ 99% by HPLC analysis. CH3 (CH2)13 O P O (CH2)2 N(CH3 )3 pH (1% solution) 5-8 _ Solubility in water at 0-5°C ≥ 20% O Conductance (10% solution) < 200 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.02 280 nm < 0.08 260 nm < 0.1 1. Anatrace measurement. PRODUCT INFOMATION 2. R. Zeisig, A. Ress, I. Fichtner, W. Walther, Cancer Gene Ther. 10, 302-311 (2003).
F312D FOS-CHOLINE®-14, DEUTERATED 100 mg 253.98
[n-Tetradecylphosphocholine-d42] 500 mg 1015.92 1 g 1828.66
FW 421.5 [869638-98-2] C19D42NO4P CMC (H O) ~ 0.12 mM (0.0051%)1 2 O + NEW PRODUCTS Purity ≥ 90% pure by HPLC analysis. CD3 (CD 2 )13 O P O (CD 2 )2 N(CD 3 )3 pH (1% solution) 4-9 _ Solubility in water at 20°C > 1% O 1. CMC value for the undeuterated compound. 2. See F312 for references.
F312S FOS-CHOLINE®-14, SOL-GRADE® 1 g 113.22 [n-Tetradecylphosphocholine] 5 g 452.88 25 g 1811.52
PRODUCT LISTING FW 379.5 [77733-28-9] C19H42NO4P CMC (H O) ~ 0.12 mM1 (0.0046%) 2 O + Purity ≥ 97% pure by HPLC analysis. CH3(CH2)13 O P O (CH2)2 N(CH3)3 pH (1% solution) 4-9 _ 10% solution may appear slightly hazy or cloudy at room temperature. O Conductance (10% solution) < 500 μS Absorbance of a 1% detergent solution: 340 nm < 0.1 280 nm < 0.2 260 nm < 0.2 1. Anatrace measurement. 2. See F312 for references. INDEX
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F314 FOS-CHOLINE®-15, ANAGRADE® 1 g 141.78 [n-Pentadecylphosphocholine] 5 g 567.12 25 g 2270.11
FW 393.5 [146801-07-2] C20H44NO4P CMC (H O) ~ 0.07 mM1 (0.0027%) 2 O
+ PRODUCT INFORMATION Purity ≥ 99% by HPLC analysis. CH3(CH2)14 O P O (CH2)2 N(CH3)3 pH (1% solution) 5-8 _ Solubility in water at 0-5°C ≥ 20% O Conductance (10% solution) <200 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.02 280 nm < 0.08 260 nm < 0.1 1. Anatrace measurement. NEW PRODUCTS
F314S FOS-CHOLINE®-15, SOL-GRADE® 1 g 113.22 [n-Pentadecylphosphocholine] 5 g 452.88 25 g 1811.52
FW 393.5 [146801-07-2] C20H44NO4P CMC (H O) ~ 0.07 mM1 (0.0027%) 2 O + Purity ≥ 97% by HPLC analysis. CH3(CH2)14 O P O (CH2)2 N(CH3)3 pH (1% solution) 4-9 _ 10% solution may appear slightly hazy or cloudy at room temperature. O Conductance (10% solution) <500 μS Absorbance of a 1% detergent solution: 340 nm < 0.1 280 nm < 0.2 260 nm < 0.2 1. Anatrace measurement.
F316 FOS-CHOLINE®-16, ANAGRADE® 1 g 141.78 [n-Hexadecylphosphocholine / 2-[[(Hexadecyloxyl)hydroxyphosphinyl]oxy]- 5 g 567.12 N,N,N-trimethyl-ethanaminium, hydroxide, inner salt] 25 g 2270.11
FW 407.5 [58066-85-6] C21H46NO4P CMC (H O) ~ 0.013 mM1 (0.00053%) 2 O Purity ≥ 99% by HPLC analysis. + CH3(CH2)15 O P O (CH2)2 N(CH3)3 pH (1% solution) 5-8 _ Solubility in water at 0-5°C ≥ 20% O Conductance (10% solution) <200 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.02 280 nm < 0.08 260 nm < 0.1
1. Anatrace measurement. INDEX
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Catalog No. Product Name/Specifications Size Price
F316S FOS-CHOLINE®-16, SOL-GRADE® 1 g 113.22 [n-Hexadecylphosphocholine / 2-[[(Hexadecyloxyl)hydroxyphosphinyl]oxy]- 5 g 452.88 N,N,N-trimethyl-ethanaminium, hydroxide, inner salt] 25 g 1811.52
FW 407.5 [58066-85-6] C21H46NO4P CMC (H O) ~ 0.013 mM1 (0.00053%) 2 O + Purity ≥ 97% by HPLC analysis. CH3(CH2)15 O P O (CH2)2 N(CH3)3 pH (1% solution) 4-9 _ 10% solution may appear slightly hazy or cloudy at room temperature. O Conductance (10% solution) < 500 μS Absorbance of a 1% detergent solution: 340 nm < 0.1 280 nm < 0.2 260 nm < 0.2 1. Anatrace measurement. PRODUCT INFOMATION FCI09 FOS-CHOLINE®-ISO-9, ANAGRADE® 1 g 141.78 [2,6-Dimethyl-4-heptylphosphocholine] 5 g 567.12 25 g 2268.48 CH3 FW 309.0 [869646-90-2] C14H32NO4P 1 O CMC (H2O) ~ 32 mM (0.99%) CH3 CH CH2 Purity 99% by HPLC analysis. + ≥ CH OPO (CH2)2 N(CH3)3 pH (1% solution) 4-9 CH3 CH CH2 NEW PRODUCTS _ Solubility in water at 0-5oC ≥ 20% O Conductance (10% solution) < 300 μS CH3 Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.05 280 nm < 0.08 260 nm < 0.10 1. Anatrace measurement. Note: This product is a mixture of closely related dimethylheptylphosphocholines. The major component is 2,6-Dimethyl-4-heptylphosphocholine (95-99%) and
PRODUCT LISTING the minor component is 4,6-Dimethyl-4-heptylphosphocholine (0-5%).
FCI11 FOS-CHOLINE®-ISO-11, ANAGRADE® 1 g 132.91 [2,8-Dimethyl-5-nonylphosphocholine] 5 g 531.63 25 g 2126.55 FW 337.4 [869647-65-4] C H NO P CH3 16 36 4 O 1 CMC (H2O) ~ 26.6 mM (0.9%) CH3 CH (CH2)2 + Purity ≥ 99% by HPLC analysis. CH OPO (CH2)2 N(CH3)3 CH CH (CH ) pH (1% solution) 5-8 3 2 2 _ Solubility in water at 0-5oC ≥ 20% O CH Conductance (10% solution) < 200 μS 3 Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.02 280 nm < 0.08 260 nm < 0.1 1. Anatrace measurement. INDEX
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FCI116 FOS-CHOLINE®-ISO-11-6U, ANAGRADE® 1 g 143.11 [Undecyl-6-phosphocholine] 5 g 572.42 25 g 2289.70
FW 337.16 [869647-81-4] C16H36NO4P CMC (H O) ~ 25.8 mM1 (0.87%) O 2 CH (CH ) 3 2 4 + PRODUCT INFORMATION Purity ≥ 99% pure by HPLC analysis. CH O P O (CH2) 2 N(CH3)3 pH (1% solution) 5-8 CH (CH ) 3 2 4 O_ Solubility in water at 0-5oC ≥ 20% Conductance (10% solution) < 200 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.05 280 nm < 0.1 260 nm < 0.2 1. Anatrace measurement.
® ®
FCU110 FOS-CHOLINE -UNSAT-11-10, ANAGRADE 1 g 132.91 NEW PRODUCTS [10-Undecylenyl-1-phosphocholine] 5 g 531.63 25 g 2126.55
FW 335.4 [121045-77-0] C16H34NO4P 1 CMC (H2O) ~ 6.2mM (0.21%) O Purity ≥ 99% by HPLC analysis. + CH 2 CH(CH2)9 O PO(CH ) N(CH ) pH (1% solution) 5-8 2 2 3 3 o % _ Solubility in water at 0-5 C ≥ 20 O Conductance (10% solution) < 200 μS Absorbance of a 1% detergent solution: 340 nm < 0.02 280 nm < 0.08 260 nm < 0.1 1. Anatrace measurement.
F208 FOS-MEA®-8, ANAGRADE® 1 g 132.91 [Octylphospho-N-methylethanolamine] 5 g 531.63 25 g 2126.55
FW 267.01 [104702-33-2] C11H26NO4P CMC (H O) ~ 22.0 mM1 (0.59%) 2 O H Purity ≥ 99% pure by HPLC analysis. CH (CH ) O PO(CH ) N CH pH (1% solution) 5-8 3 2 7 2 2 3 Solubility in water at 0-5oC ≥ 1% OH Conductance (10% solution) < 200 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.05 280 nm < 0.08 260 nm < 0.1 1. Anatrace measurement. INDEX
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Catalog No. Product Name/Specifications Size Price
F210 FOS-MEA®-10, ANAGRADE® 1 g 132.91 [Decylphospho-N-methylethanolamine] 5 g 531.63 25 g 2126.55
FW 295.01 [557788-85-9] C13H30NO4P 1 CMC (H2O) ~ 5.25 mM (0.15%) O H Purity ≥ 99% pure by HPLC analysis. pH (1% solution) 3-8 CH3 (CH2)9 O P O (CH2 )2 N CH3 o Solubility in water at 20 C ≥ 1% OH Conductance (1% solution) < 200 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.05 280 nm < 0.08 260 nm < 0.1 1. Anatrace measurement. PRODUCT INFOMATION
F212 FOS-MEA®-12, ANAGRADE® 1 g 132.91 [Dodecylphospho-N-methylethanolamine] 5 g 531.63 25 g 2126.55
FW 323.01 [129274-39-1] C15H34NO4P CMC (H O) ~ 0.43 mM1 (0.014%) 2 O H Purity ≥ 99% pure by HPLC analysis. CH3(CH2)11 O P O (CH2)2 N CH3 NEW PRODUCTS pH (0.1% solution) 3-8 Solubility in water at 20oC ≥ 0.01% OH Conductance (0.1% solution) < 200 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 0.1% detergent solution: 340 nm < 0.05 280 nm < 0.08 260 nm < 0.1 1. Anatrace measurement.
™ ®
PRODUCT LISTING FF360 FOSFEN -9, ANAGRADE Please inquire. [Nonylphenylphosphocholine]
FW 385.21 [869648-98-6] C20H36NO4P 1 CMC (H2O) ~ 1.35 mM (0.052%) 95% pure by HPLC analysis.2 pH (1% solution) 4-9 O + Solubility in water at 0-5oC ≥ 20% CH3 (CH2)8 O P O (CH2)2 N(CH3)3 Conductance (10% solution) < 500 μS _ O 1. Anatrace measurement. 2. Product is a mixture of alkylphenylphosphocholines. The major component is the para substituted product while the minor components are the meta and ortho substituted products. INDEX
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Catalog No. Product Name/Specifications Size Price
G1000 GUANIDINE HYDROCHLORIDE 100 g 36.00 500 g 101.00 . FW 95.53 [50-01-1] (NH2)2C=NH HCl Form: White clear crystals Assay (elemental analysis): ≥ 99.5%
Absorbance (260nm, 6M, 1cm): < 0.03 PRODUCT INFORMATION Absorbance (225nm, 6M, 1cm): < 0.2 Melting Point: 184 + 3°C NH HCl Solubility (6M H O): Clear and colorless 2 H NNH Aldehyde: None detected 2 2
Sulfate (SO4): < 50 ppm Arsenic (As): < 1 ppm Copper (Cu): < 1 ppm Iron (Fe): < 2 ppm Lead (Pb): < 5 ppm Zinc (Zn): < 1 ppm Endonuclease: None detected NEW PRODUCTS Ribonuclease: None detected Protease: None detected
H108 HEGA®-8, ANAGRADE® 1 g 79.31 [Octanoyl-N-hydroxyethylglucamide] 5 g 317.24 25 g 1268.96
FW 351.5 [869652-63-1] C16H33NO7 O 1 CMC (H2O) ~ 109 mM (3.8%) CH 3 (CH 2 )6 C N (CH 2 )2 OH Purity ≥ 99% by HPLC analysis. pH (1% solution) 5-8 CH 2 Solubility in water at 0-5°C ≥ 20% H C OH
Conductance (10% solution) < 100 μS HO C H Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 H C OH Absorbance of a 1% detergent solution: 340 nm < 0.05 280 nm < 0.08 H C OH
260 nm < 0.1 CH 2 OH 1. Anatrace measurement.
H109 HEGA®-9, ANAGRADE® 1 g 79.31 [Nonanoyl-N-hydroxyethylglucamide] 5 g 317.24 25 g 1268.96 FW 365.5 [869653-90-7] C H NO 17 35 7 O 1 CMC (H2O) ~ 39 mM (1.4%) Purity ≥ 99% by HPLC analysis. CH 3 (CH 2 )7 C N (CH 2 )2 OH
pH (1% solution) 5-8 CH 2 Solubility in water at 0-5°C 20% ≥ H C OH Conductance (10% solution) < 100 μS HO C H
Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 INDEX
Absorbance of a 1% detergent solution: 340 nm < 0.05 H C OH 280 nm < 0.08 OH 260 nm < 0.1 H C
1. Anatrace measurement. CH 2 OH
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Catalog No. Product Name/Specifications Size Price
H110 HEGA®-10, ANAGRADE® 1 g 79.31 [Decanoyl-N-hydroxyethylglucamide] 5 g 317.24 25 g 1268.96
FW 379.5 [139361-84-5] C18H37NO7 1 O CMC (H2O) ~ 7.0 mM (0.26%)
Purity ≥ 99% by HPLC analysis. CH3(CH2)8 C N (CH2)2OH pH (1% solution) 5-8 CH Solubility in water at 0-5°C ≥ 10% 2 Conductance (10% solution) < 100 μS H C OH Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 HO C H Absorbance of a 1% detergent solution: 340 nm < 0.05 280 nm < 0.08 H C OH
260 nm < 0.1 H C OH NOTE: Heating may be required to dissolve the detergent. PRODUCT INFOMATION 1. Anatrace measurement. CH2OH 2. Cortes, D. M. and Perozo, E., Biochem. 36, 10343-10352 (1997).
H111 HEGA®-11, ANAGRADE® 1 g 79.31 [Undecanoyl-N-hydroxyethylglucamide] 5 g 317.24 25 g 1268.96
FW 393.5 [869654-10-4] C19H39NO7 O 1 CMC (H2O) ~ 1.4 mM (0.055%) NEW PRODUCTS CH 3 (CH 2 )9 C N (CH 2 )2 OH Purity ≥ 99% by HPLC analysis. pH (0.5% solution) 5-8 CH 2 Solubility in water at 0-5°C ≥ 0.5% H C OH Conductance (0.5% solution) < 100 μS HO C H Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 0.5% detergent solution: 340 nm < 0.05 H C OH
280 nm < 0.08 H C OH 260 nm < 0.1 1. Anatrace measurement. CH 2 OH PRODUCT LISTING
H300 n-HEPTYL-β-D-GLUCOPYRANOSIDE, ANAGRADE® 1 g 58.43 [n-Heptyl-β-D-glucoside] 5 g 233.71 25 g 934.84
FW 278.4 [78617-12-6] C13H26O6 1 CMC (H2O) ~ 70 mM (1.9%) Purity ≥ 99% by HPLC analysis. Percent alpha < 2 (HPLC) Percent heptanol < 0.005 (HPLC) CH2OH pH (1% solution) 5-8 O O (CH2)6CH3 Solubility in water at 0-5°C ≥ 20% OH Conductance (10% solution) < 40 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 HO Absorbance of a 1% detergent solution: 340 nm < 0.02 OH 280 nm < 0.04
INDEX 260 nm < 0.06 225 nm < 0.1 1. Anatrace measurement.
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Catalog No. Product Name/Specifications Size Price
H300LA n-HEPTYL-β-D-GLUCOPYRANOSIDE, ANAGRADE® 1 g 75.92 [n-Heptyl-β-D-glucoside] 5 g 303.68 (Contains almost no alpha isomer) 25 g 1214.72
FW 278.4 [78617-12-6] C13H26O6 1 CMC (H2O) ~ 70 mM (1.9%) CH2OH
Purity 99% by HPLC analysis. PRODUCT INFORMATION ≥ O O (CH2)6CH3 Percent alpha < 0.1 (HPLC) OH Percent heptanol < 0.005 (HPLC) pH (1% solution) 5-8 HO Solubility in water at 0-5°C ≥ 20% OH Conductance (10% solution) < 40 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.02 280 nm < 0.04 260 nm < 0.06 225 nm < 0.1
1. Anatrace measurement. NEW PRODUCTS
H301 n-HEPTYL-β-D-THIOGLUCOPYRANOSIDE, ANAGRADE® 1 g 60.52 [n-Heptyl-β-D-thioglucoside] 5 g 226.97 25 g 851.09
FW 294.4 [85618-20-8] C13H26O5S 1 CMC (H2O) ~ 29 mM (0.85%) Gummy solid. CH2OH O Purity ≥ 99% by HPLC analysis. S (CH2)6CH3 Percent alpha < 2 (HPLC) OH pH (1% solution) 5-8 HO Solubility in water at 0-5°C ≥ 10% OH Conductance (10% solution) < 100 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 0.5% detergent solution: 340 nm < 0.1 280 nm < 0.2 260 nm < 0.25 1. Anatrace measurement.
H301LA n-HEPTYL-β-D-THIOGLUCOPYRANOSIDE, ANAGRADE® 1 g 78.68 [n-Heptyl-β-D-thioglucoside] 5 g 314.69 (Contains almost no alpha isomer) 25 g 1258.76
FW 294.4 [85618-20-8] C13H26O5S 1 CMC (H2O) ~ 29 mM (0.85%) Gummy solid. CH2OH O Purity ≥ 99% by HPLC analysis. S (CH2)6CH3 Percent alpha < 0.1 (HPLC) OH pH (1% solution) 5-8 HO Solubility in water at 0-5°C > 10% OH Conductance (10% solution) < 100 μS INDEX Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.1 280 nm < 0.2 260 nm < 0.25 1. Anatrace measurement. 800-252-1280 • 419-891-3030 • FAX 419-891-3037 • www.anatrace.com 69 Product Listing
Catalog No. Product Name/Specifications Size Price
H320 n-HEXADECYL-β-D-MALTOPYRANOSIDE, ANAGRADE® 1 g 111.40 [n-Hexadecyl-β-D-maltoside] 5 g 417.77 25 g 1566.62
FW 566.6 [98064-96-1] C28H54O11 1 CMC (H2O) ~ 0.0006 mM (0.00003%) CH2OH CH2OH Purity 97% pure by HPLC analysis. ≥ O O O (CH2)15 CH3 Percent alpha < 5 (HPLC) OH Percent hexadecanol < 0.01 (HPLC) OH HO O pH (0.1% solution) 5-8 Solubility in water at 40°C ≥ 1.0% OH OH Conductance (0.1% solution) < 80 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 0.1% detergent solution: 340 nm < 0.05 280 nm < 0.1 PRODUCT INFOMATION 260 nm < 0.15 225 nm < 0.3 NOTE: Heating may be required to dissolve detergent. 1. Anatrace estimate.
H355 HEXADECYLTRIMETHYLAMMONIUM CHLORIDE 5 g 20.28 25 g 81.12 FW 320.00 [112-02-7] C H ClN 100 g 259.58 NEW PRODUCTS 19 42 CH 1 3 CMC (H2O) ~ 0.0032 mM (0.000102%) 3-4 + Aggregation Number (H2O) ~ 61-84 CH3 N (CH2)15 CH3 Purity ≥ 95% pure by titration. pH (1% solution) 5.5-8.5 - CH3 Cl Solubility in water at 20°C ≥ 30% Absorbance of a 1% detergent solution: 340 nm < 0.02 280 nm < 0.04 260 nm < 0.06 225 nm < 0.08
PRODUCT LISTING 1. Anatrace measurement. 2 H. Matsubara, T. Nakano, T. Matsuda, et al, Langmuir 21, 8131-8137 (2005). 3. Maillet, J.-B., Lachet, V., and Coveney, P. V., Phys. Chem. Chem. Phys. 1, 5277-5290 (1999). 4. Neugebauer, J. M., Methods Enzymol. 182, 239-253 (1990).
H350 HEXAETHYLENE GLYCOL MONOOCTYL ETHER, ANAGRADE® 2 ml (1 ampule) 66.61
[C8E6] 10 ml (1 ampule) 266.43 50 ml (5 ampules) 1065.71
FW 394.5 [4440-54-4] C20H42O7 1 CMC (H2O) ~ 10 mM (0.39%) 1 Aggregation Number (H2O) ~ 32 Supplied as a 50% (w/w) solution under argon. Purity ≥ 99% by HPLC analysis. CH3(CH2)7O(CH2CH2O)6H Purified to contain < 500 μM of equivalent peroxide. pH (1% solution) 5-8 Conductance (10% solution) < 50 μS
INDEX 1. LeMaire, M., Champeil, P. and Moller, J. V., Biochimica et Biophysica Acta 1508, 86-111 (2000).
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Catalog No. Product Name/Specifications Size Price
H305 n-HEXYL-β-D-GLUCOPYRANOSIDE, ANAGRADE® 1 g 58.43 [n-Hexyl-β-D-glucoside] 5 g 233.71 25 g 934.84
FW 264.4 [59080-45-4] C12H24O6 1 CMC (H2O) ~ 250 mM (6.6%) CH2OH Purity ≥ 99% by HPLC analysis. PRODUCT INFORMATION O (CH ) CH For molar volume check reference 2. O 2 5 3 Percent alpha < 2 (HPLC) OH Percent hexanol < 0.005 (HPLC) HO pH (1% solution) 5-8 OH Solubility in water at 0-5°C ≥ 20% Conductance (10% solution) < 40 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.02 280 nm < 0.04 260 nm < 0.06 NEW PRODUCTS 225 nm < 0.1 1. Anatrace measurement. 2. Brown, G. M., Dubreuil, P., Ichhaporia, F. M. and Desnoyers, J. E., Canadian J. Chem. 48, 2525-2531 (1970).
H310 n-HEXYL-β-D-MALTOPYRANOSIDE, ANAGRADE® 1 g 59.99 [n-Hexyl-β-D-maltoside] 5 g 224.95 25 g 843.56
FW 426.4 [870287-95-9] C18H34O11 1 CH OH CH OH CMC (H2O) ~ 210 mM (8.9%) 2 2 Purity ≥ 99% by HPLC analysis. O O O (CH2)5CH3 Percent alpha < 2 (HPLC) OH OH Percent hexanol < 0.005 (HPLC) HO O pH (1% solution) 5-8 OH Solubility in water at 0-5°C ≥ 20% OH Conductance (10% solution) < 40 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.02 280 nm < 0.04 260 nm < 0.06 225 nm < 0.1 1. Anatrace measurement.
I1001 IMIDAZOLE 25 g 12.00 100 g 20.00 500 g 36.00
FW 68.08 [288-32-4] C3H4N2 White to cream crystalline flakes/powder N Assay (Titration): ≥ 98.5% (dry basis) NH pKa (25°C): 6.95 + 0.15 Melting Point: No more than a three degree range which includes 90°C INDEX Iron (Fe): < 30 ppm Heavy Metals (Pb): < 10 ppm
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Catalog No. Product Name/Specifications Size Price
I1002 ISOPROPYL-β-D-THIOGALACTOPYRANOSIDE, ANAGRADE® 1 g 30.60 [IPTG / 1-Methylethyl-1-thio-β-D-galactopyranoside] 5 g 122.91 25 g 430.32
FW 238.31 [367-93-1] C9H18O5S White solid CH2OH SCH(CH ) Purity ≥ 99% by HPLC analysis. OH O 3 2 Conductance of a 10% solution < 40 μS OH Absorbance of a 1% solution: 340 nm < 0.04
280 nm < 0.04 OH 260 nm < 0.06 No dioxane detected by HPLC. PRECAUTIONS: Protect from light. Store frozen and keep dry. Warm to room temperature before opening.
I1003 ISOPROPYL-β-D-THIOGALACTOPYRANOSIDE, ANALYTICAL GRADE 5 g 32.64 PRODUCT INFOMATION [IPTG / 1-Methylethyl-1-thio-β-D-galactopyranoside] 25 g 122.40 100 g 342.72
FW 238.31 [367-93-1] C9H18O5S White solid CH2OH Purity 99% by HPLC analysis. SCH(CH ) ≥ OH O 3 2 Conductance of a 10% solution < 200 μS OH Absorbance of a 1% solution: 340 nm < 0.1
NEW PRODUCTS 280 nm < 0.1 260 nm < 0.2 OH No dioxane detected by HPLC. PRECAUTIONS: Protect from light. Store frozen and keep dry. Warm to room temperature before opening.
I1003P ISOPROPYL-β-D-THIOGALACTOPYRANOSIDE, ANALYTICAL GRADE 5 g 33.66 [IPTG / 1-Methylethyl-1-thio-β-D-galactopyranoside] 25 g 134.64 Source: Plant 100 g 403.92
FW 238.31 [367-93-1] C9H18O5S
PRODUCT LISTING White solid CH2OH Purity 99% by HPLC analysis. SCH(CH ) ≥ OH O 3 2 Conductance of a 10% solution < 200 μS OH Absorbance of a 1% solution: 340 nm < 0.1 280 nm < 0.1 260 nm < 0.2 OH No dioxane detected by HPLC. PRECAUTIONS: Protect from light. Store frozen and keep dry. Warm to room temperature before opening.
IPTG is an inducer of the lac operon 1 and is often used in conjunction with a b-galactosidase substrate such as X-GAL (pg. 107) for determination of the lac phenotype in recombinant organisms. Some IPTG References: 1. Baim, S. B., Labow, A. J. Levine and Shenk, T., A chimeric mammalian transactivator based on the lac repressor that is regulated by temperature and isopropyl-D-thiogalactopyranoside, Proc. Natl. Acad. Sci. USA 88, 5072-7056 (1991). 2. Pestov, D. G. and Lau, L. F., Genetic selection of groth-inhibitory sequences in mammalian cells. Proc. Natl. Acad. Sci. USA 91, 12549-12553 (1994). INDEX 3. Yanzhuang, L. and Lau, L. F., IPTG-Inducible Episomal Expression System for Exogenous Genes in Primate Cells, BioTechniques 28, 577-581 (2000).
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Catalog No. Product Name/Specifications Size Price
L1000 LB BROTH, READY-MADE POWDER 250 g 16.00
Form: Off-white powder
pH (2.0%, H2O): 7.0 + 0.2 Contents: Casein Peptone: 10gm/L
Yeast Extract: 5gm/L PRODUCT INFORMATION Sodium Chloride: 5gm/L To use: Dissolve 20gm powder per liter of purified water. For the cultivation of E. coli. Low salt formulation is ideal for culturing in the presence of salt-sensitive antibiotics.
L360S LAPAO, SOL-GRADE® 1 g 50.96 [3-Dodecylamido-N,N'-dimethylpropyl aminoxide / 5 g 203.84 3-Laurylamido-N,N'-dimethylpropyl aminoxide] 25 g 815.36
FW 300.55 [61792-31-2] C17H36N2O2 CMC (H O) ~ 1.56 mM1 (0.052%) O CH3
2 NEW PRODUCTS 1 Aggregation Number (H2O) ~ 126 CH3(CH2)10 CNH(CH2)3 N O
Purity ≥ 95% by HPLC analysis. CH3 Purified to contain < 1000 μM of equivalent peroxide. Color: Pale Yellow pH (1% solution) 4-9 Solubility in water at 20oC ≥ 20% Conductance (10% solution) < 500 μS Absorbance of a 1% detergent solution: 340 nm < 0.2 280 nm < 0.3 260 nm < 0.4 1. Personal communication from Professor Ronald S. Kaplan, Chicago Medical School. 2. C. Dahout-Gonzalez, G. Brandolin, E. Pebay-Peyroula, Acta. Cryst. D59, 2353-2355 (2003).
L1002 LITHIUM DODECYL SULFATE (LDS) 5 g 21.00 25 g 71.00
FW 272.33 [2044-56-6] CH3(CH2)11 OSO3Li Form: White crystalline powder
Assay (C12 by GC): ≥ 98% Absorbance (280nm, 3%, 1cm): < 0.1 O Absorbance (230nm, 3%, 1cm): < 0.2 O S OLi Loss on Drying: < 1% Chloride (Cl): < 0.01% 11 O Copper (Cu): < 5 ppm Iron (Fe): < 1 ppm Lead (Pb): < 5 ppm May be used for electrophoresis in cold conditions. INDEX
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Catalog No. Product Name/Specifications Size Price
L1001 LURIA BROTH, READY-MADE POWDER 250 g 15.00
Form: Off-white powder
pH (2.5%, H2O): 7.0 + 0.2 Contents: Casein Peptone: 10gm/L Yeast Extract: 5gm/L Sodium Chloride: 10gm/L To use: Dissolve 25gm powder per liter of purified water. Autoclave. For the cultivation of E. coli.
B2010 MAGENTA-GAL 50 mg 22.88 [5-Bromo-6-chloro-3-indolyl-β-D-galactopyranoside] 250 mg 91.52 H
FW 408.6 [93863-88-8] C14H15BrClNO6 N Cl PRODUCT INFOMATION
Purity ≥ 95% pure by HPLC analysis. CH2OH 24 Br Specific Rotation [α] D (c=1.3, EtOH 96%) -44° ± 3° OH O O Melting Point: 187-191°C OH Solubility > 1% in EtOH
IR spectrum conforms to specifications. OH
M319 MEGA-8, ANAGRADE® 1 g 31.78 NEW PRODUCTS [Octanoyl-N-methylglucamide] 5 g 127.13 25 g 508.53 FW 321.4 [85316-98-9] C H NO 15 31 6 O 1,2 CMC (H2O) ~ 79 mM (2.5%) CH3 (CH2)6 C N CH3 Purity ≥ 99% by HPLC analysis.
pH (1% solution) 5-8 CH2 Solubility in water at 0-5°C ≥ 20% Conductance (10% solution) < 80 μS H C OH Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 HO C H PRODUCT LISTING Absorbance of a 1% detergent solution: 340 nm < 0.05 280 nm < 0.08 H C OH 260 nm < 0.1 1. Anatrace measurement. H C OH 2. Y. Mine, K. Fukunaga, N. Maruoka, et al, J. Biosci. Bioeng. 90, 631-636 (2000). 3. Hanatani, M., et al, J. Biochem. 95, 1349-1353 (1984). CH2OH 4. Hildreth, J. E. K., Biochem. J. 207, 363 (1982). INDEX
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Catalog No. Product Name/Specifications Size Price
M325 MEGA-9, ANAGRADE® 1 g 31.78 (Nonanoyl-N-methylglucamide) 5 g 127.13 25 g 508.53
FW 335.5 [85261-19-4] C16H33NO6 1,2 CMC (H2O) ~ 25 mM (0.84%) O Purity ≥ 99% by HPLC analysis. PRODUCT INFORMATION CH3 (CH2)7 C N CH3 pH (1% solution) 5-8 Solubility in water at 5°C ≥ 5% CH2 Conductance (5% solution) < 80 μS H C OH Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.05 HO C H 280 nm < 0.08 H C OH 260 nm < 0.1 H C OH 1. Anatrace measurement. 2. Hanatani, M., et al, J. Biochem. 95, 1349-1353 (1984). CH2OH 3. Hildreth, J. E. K., Biochem. J. 207, 363 (1982). NEW PRODUCTS
M320 MEGA-10, ANAGRADE® 1 g 31.78 [Decanoyl-N-methylglucamide] 5 g 127.13 25 g 508.53
FW 349.5 [85261-20-7] C17H35NO6 1,4 CMC (H2O) ~ 6-7 mM (0.21%) O
Purity ≥ 99% by HPLC analysis. CH3 (CH2 )8 C N CH3 pH (0.3% solution) 5-8 Solubility in water at 0-5°C ≥ 0.3% CH2 Conductance (0.3% solution) < 40 μS H C OH Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 0.3% detergent solution: 340 nm < 0.05 HO C H
280 nm < 0.08 H C OH 260 nm < 0.1 1. Anatrace measurement. H C OH 2. M. A. Churchward, R. H. Butt, J. C. Lang, et al, Proteome Sci. 3, 5 (2005). 3. J. M. Hierrezuelo, J. Aguiar, C. C. Ruiz, Langmuir 20, 10419-10426 (2004). CH2OH 4. Hanatani, M., et al, J. Biochem. 95, 1349-1353 (1984). 5. Hildreth, J. E. K., Biochem. J. 207, 363 (1982).
M2500 MICROPIPETTES (25μL) 250 49.00 - glass
M110MT MMTS 100 mg 68.00 [Methyl methanethiosulfonate] 1 g 540.00 H C 3 S FW 126.20 [2949-92-0] C H O S 2 6 2 2 OOS Form: Light yellow liquid CH3 Boiling point: 69-70°C / 0.4mm INDEX
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Catalog No. Product Name/Specifications Size Price
O110MT MTSL 10 mg 60.00 [(1-Oxyl-2,2,5,5-tetramethyl-D3-pyrroline-3-methyl) methanethiosulfonate] 100 mg 480.00 O CH3 FW 264.30 [N/A] C H NO S S 10 18 3 2 S Form: Light yellow powder O Melting point: 107-108°C N O
ND195 NDSB-195 5 g 41.34 [Dimethylethylammoniumpropanesulfonate] 25 g 165.36
CH3 100 g 529.15 FW 195.3 [160255-06-1] C H NO S _ 7 17 3 CH3CH2 N (CH2)3SO3 Solubility in water ≥ 10% + CH3 PRODUCT INFOMATION IR spectrum conforms to specifications. Elemental analysis supplied with each lot (C,H,N).
ND201 NDSB-201 25 g 20.14 [(3-1-Pyridino)-1-propane sulfonate / 100 g 64.45 1-(3-Sulfopropyl)-pyridinium hydroxide, inner salt] 500 g 257.79
FW 201.2 [15471-17-7] C8H11NO3S NEW PRODUCTS _ Solubility in water ≥ 10% + N (CH2)3 SO3 IR spectrum conforms to specifications. Elemental analysis supplied with each lot (C,H,N).
ND211 NDSB-211 1 g 43.46 [Dimethyl(2-hydroxyethyl)ammonium-1-propanesulfonate] 5 g 173.84 CH3 25 g 695.36 _ FW 211.3 [38880-58-9] C7H17NO4S CH3 N (CH2 )3SO3 Solubility in water ≥ 10% + PRODUCT LISTING IR spectrum conforms to specifications. (CH2)2 Elemental analysis supplied with each lot (C,H,N). OH
ND221 NDSB-221 5 g 41.34 [3-(1-Methylpiperidinium)-1-propane sulfonate] 25 g 165.36 100 g 529.15
FW 221.3 [160788-56-7] C9H19NO3S CH3 Solubility in water > 10% N _ IR spectrum conforms to specifications. + (CH2)3 SO3 Elemental analysis supplied with each lot (C,H,N).
ND256 NDSB-256 5 g 41.34 [Dimethylbenzylammoniumpropanesulfonate] 25 g 165.36 CH3 100 g 529.15 _ FW 257.4 [81239-45-4] C H NO S 12 19 3 CH2 N (CH2)3 SO3 INDEX Solubility in water ≥ 10% + IR spectrum conforms to specifications. CH3 Elemental analysis supplied with each lot (C,H,N).
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Catalog No. Product Name/Specifications Size Price NIDP40 NONIDET P40 SUBSTITUTE (see Cat. # APND40, pg. 24) 500 mL 31.00 (Igepal CA-630 / [Octylphenoxy]polyethoxyethanol) 1 L 44.00
FW avg. 603 [2497-59-8] CMC (50mM Na+) ~ 0.05-0.3 mM1 1 Aggregation Number (H2O) ~ 100-155
OH PRODUCT INFORMATION Density: 1.060 + 0.005 O Clear, slightly yellow-green viscous liquid. Chemically indistinguishable from Nonidet P40, which is no longer commercially available. 1. Black, Shaun D.: http://psyche.uthct.edu/shaun/SBlack/detergnt.html
N324 n-NONYL-β-D-GLUCOPYRANOSIDE, ANAGRADE® 1 g 58.43 [n-Nonyl-β-D-glucoside] 5 g 233.71 25 g 934.84
FW 306.4 [69984-73-2] C15H30O6 1 2 1 CMC (H2O) ~ 6.5 mM (0.20%) CMC (0.15M NaCl) ~ 6 mM CMC (1M NaCl) ~ 3.5 mM NEW PRODUCTS Purity ≥ 99% by HPLC analysis. Percent alpha < 2 (HPLC) CH2OH Percent nonanol < 0.005 (HPLC) O O (CH2)8CH3 pH (1% solution) 5-8 OH Solubility in water at 0-5°C ≥ 20% Conductance (10% solution) < 40 μS HO Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 OH Absorbance of a 1% detergent solution: 340 nm < 0.02 280 nm < 0.04 260 nm < 0.06 225 nm < 0.1 1. Anatrace measurement. 2. H. Zer, M. Vink, S. Shochat, et al, Biochemistry 42, 728-738 (2003). 3. Ostermeier, C., Harrenga, A., Ermler, U. and Michel, H., Proc. Natl. Acad. Sci. USA 94, 10547-10553 (1997). 4. Mechref, Y. and Rassi, Z. E., J. Chromatography 757, 263-273 (1997). 5. DeGrip, W. J. and Bovee-Geurts, P. H. M., Chem. Phys. Lipids 23, 312-325 (1979).
N324LA n-NONYL-β-D-GLUCOPYRANOSIDE, ANAGRADE® 1 g 84.24 [n-Nonyl-β-D-glucoside] 5 g 336.96 (Low alpha) 25 g 1347.84
FW 306.4 [69984-73-2] C15H30O6 1 2 1 CMC (H2O) ~ 6.5 mM (0.20%) CMC (0.15M NaCl) ~ 6 mM CMC (1M NaCl) ~ 3.5 mM Purity ≥ 99% by HPLC analysis. CH2OH Percent alpha < 0.4 (HPLC) O (CH ) CH Percent nonanol < 0.005 (HPLC) O 2 8 3 pH (1% solution) 5-8 OH Solubility in water at 0-5°C ≥ 20% HO Conductance (10% solution) < 40 μS OH Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 INDEX Absorbance of a 1% detergent solution: 340 nm < 0.02 280 nm < 0.04 260 nm < 0.06 225 nm < 0.1 1. See N324 for references. 800-252-1280 • 419-891-3030 • FAX 419-891-3037 • www.anatrace.com 77 Product Listing
Catalog No. Product Name/Specifications Size Price
N324S n-NONYL-β-D-GLUCOPYRANOSIDE, SOL-GRADE® 1 g 45.72 [n-Nonyl-β-D-glucoside] 5 g 182.88 25 g 731.56
FW 306.4 [69984-73-2] C15H30O6 1 2 1 CMC (H2O) ~ 6.5 mM (0.20%) CMC (0.15M NaCl) ~ 6 mM CMC (1M NaCl) ~ 3.5 mM Purity ≥ 97% pure by HPLC analysis. Percent alpha < 5 (HPLC) CH2OH Percent nonanol < 0.05 (HPLC) O O (CH2)8CH3 pH (1% solution) 4-9 OH Solubility in water at 20°C ≥ 20% HO Conductance (10% solution) < 100 μS Absorbance of a 1% detergent solution: 340 nm < 0.05 OH 280 nm < 0.1
PRODUCT INFOMATION 260 nm < 0.1 225 nm < 0.2 1. See N324 for references.
N330 n-NONYL-β-D-MALTOPYRANOSIDE, ANAGRADE® 1 g 58.60 [n-Nonyl-β-D-maltoside] 5 g 219.75 25 g 824.08
FW 468.5 [106402-05-5] C21H40O11
NEW PRODUCTS 1 CMC (H2O) ~ 6 mM (0.28%) Aggregation Number (100 mM NaCl, 20 mM HEPES pH 7.5)2 ~ 55
Purity ≥ 99% by HPLC analysis. CH2OH CH2OH Percent alpha < 2 (HPLC) O O O (CH2)8CH3 Percent nonanol < 0.005 (HPLC) OH pH (1% solution) 5-8 OH HO O Solubility in water at 0-5°C ≥ 20% Conductance (10% solution) < 40 μS OH OH Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10
PRODUCT LISTING Absorbance of a 1% detergent solution: 340 nm < 0.02 280 nm < 0.04 260 nm < 0.06 225 nm < 0.1 1. Anatrace measurement. 2. Anatrace measurement in collaboration with Professor R. M. Garavito (Michigan State University). INDEX
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Catalog No. Product Name/Specifications Size Price
N335 n-NONYL-β-D-THIOGLUCOPYRANOSIDE, ANAGRADE® 1 g 82.13 [n-Nonyl-β-D-thioglucoside] 5 g 307.99 25 g 1154.93
FW 322.4 [98854-15-0] C15H30O5S CMC (water:methanol1) ~ 2.9 mM2 (0.093%) CH2OH O PRODUCT INFORMATION Purity ≥ 99% by HPLC analysis. S (CH2)8CH3 Percent alpha < 2 (HPLC) 1 OH pH (0.05% solution ) 5-8 HO Solubility in water at 0-5°C1 ≥ 0.05% OH Conductance (0.05% solution1) < 40 μS Percent fluorescence due to a 0.05% detergent solution1 at 345 nm < 10 Absorbance of a 0.05% detergent solution1: 340 nm < 0.1 280 nm < 0.2 260 nm < 0.25 1. Solvent: (1:1) v/v methanol : water. 2. Anatrace measurement. NEW PRODUCTS
N350 n-NONYL-β-D-THIOMALTOPYRANOSIDE, ANAGRADE® 1 g 82.13 (n-Nonyl-β-D-thiomaltoside) 5 g 307.99 25 g 1154.93
FW 484.6 [148565-55-3] C21H40O10S 1 CMC (H2O) ~ 3.2 mM (0.15%) CH2OH CH2OH
Purity ≥ 99% by HPLC analysis. O O S (CH2)8CH3 Percent alpha < 2 (HPLC) OH OH pH (1% solution) 5-8 HO O Solubility in water at 0-5°C 20% ≥ OH OH Conductance (10% solution) < 40 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.05 280 nm < 0.15 260 nm < 0.2 1. Anatrace measurement. 2. Mechref, Y. and Rassi, Z. E., J. Chromatography 757, 263-273 (1997).
N150 NOPOL-FOS™, ANAGRADE® 1 g 132.91 [6,6-Dimethylbicyclo[3.1.1]hept-2-ene-2-ethylphosphocholine] 5 g 531.63 25 g 2126.55
FW 331.4 [869660-84-4] C16H30NO4P CMC (H O) ~ 42.5 mM1 (1.4%) 2 O Purity ≥ 99% by HPLC analysis. + CH2CH2 O P O (CH2)2N(CH3)3 pH (1% solution) 5-8 _ Solubility in water at 0-5oC ≥ 20% O Conductance (10% solution) < 200 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10
Absorbance of a 1% detergent solution: 340 nm < 0.02 INDEX 280 nm < 0.2 260 nm < 0.3 1. Anatrace measurement.
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Catalog No. Product Name/Specifications Size Price
O355 OCTADECYLTRIMETHYLAMMONIUM CHLORIDE 5 g 20.28 25 g 81.12
FW 348.1 [112-03-8] C21H46ClN 100 g 259.58 1 CH3 CMC (H2O) < 0.03 mM (0.001%) + Purity ≥ 95% pure by titration. CH3 N (CH2)17 CH3 pH (0.05% solution) 5.5-8.5 Solubility in water at 20°C ≥ 0.05% - CH3 Cl Absorbance of a 0.05% detergent solution: 340 nm < 0.02 280 nm < 0.04 260 nm < 0.06 225 nm < 0.08 1. Anatrace measurement. 2. N. O. McHedlov-Petrossyan, N. A. Vodolazkaya, A. A. Kornienko, et al, Langmuir 21, 7090-7096 (2005). 3. Y. Jiang, W. Qian, J.W. Hawes, J. P. Walsh, J. Biol. Chem. 275, 34092-34099 (2000). 4. Maillet, J.-B., Lachet, V., and Coveney, P. V., Phys. Chem. Chem. Phys. 1, 5277-5290 (1999). PRODUCT INFOMATION 5. Neugebauer, J. M., Methods Enzymol. 182, 239-253 (1990).
O330 OCTAETHYLENE GLYCOL MONODODECYL ETHER, ANAGRADE® 4 ml (1 ampule) 79.70
[C12E8 / Dodecyl octaethylene glycol ether / Dodecyl octaglycol / 20 ml (2 ampules) 318.80 3,6,9,12,15,18,21,24-Octaoxahexatriacontan-1-ol] 100 ml (10 ampules) 1275.20
FW 538.77 [3055-98-9] C28H58O9 1,2 CMC (0.01 M TES, pH 7.5, 0.05 M NaCl, 0.1 mM CaCl2) ~ 0.09 mM (0.0048%) NEW PRODUCTS 2 Aggregation Number (0.01 M TES, pH 7.5, 0.05 M NaCl, 0.1 mM CaCl2) ~ 90-120 Supplied as a 25% (w/w) aqueous solution under argon.
Purity ≥ 99% by HPLC analysis. CH3(CH2)11 O(CH2CH2O)8H Purified to contain < 500 μM of equivalent peroxide. pH (1% solution) 3-7 Conductance (10% solution) < 100 μS Solubility > 25% at room temperature 1. LeMaire, M., Kwee, S., Andersen, J. P. and Miller, J. V., Eur. J. Biochem. 129, 525-532, (1983).
PRODUCT LISTING 2. LeMaire, M., Champeil, P. and Moller, J. V., Biochimica et Biophysica Acta 1508, 86-111 (2000).
O330A OCTAETHYLENE GLYCOL MONODODECYL ETHER, ANALYTICAL GRADE 4 ml (1 ampule) 41.00
[C12E8 / Dodecyl octaethylene glycol ether / Dodecyl octaglycol / 20 ml (2 ampules) 164.00 3,6,9,12,15,18,21,24-Octaoxahexatriacontan-1-ol] 100 ml (10 ampules) 656.00
FW 538.77 [3055-98-9] C28H58O9 1,2 CMC (0.01 M TES, pH 7.5, 0.05 M NaCl, 0.1 mM CaCl2) ~ 0.09 mM (0.0048%) 2 Aggregation Number (0.01 M TES, pH 7.5, 0.05 M NaCl, 0.1 mM CaCl2) ~ 90-120 Supplied as a 25% (w/w) aqueous solution under argon. Purity ≥ 97% pure by HPLC analysis. pH (1% solution) 3-7 CH3(CH2)11 O(CH2CH2O)8H Conductance (10% solution) < 500 μS Solubility > 25% at room temperature Caution: Contains organic peroxides. 1. LeMaire, M., Kwee, S., Andersen, J. P. and Miller, J. V., Eur. J. Biochem. 129, 525-532, (1983).
INDEX 2. LeMaire, M., Champeil, P. and Moller, J. V., Biochimica et Biophysica Acta 1508, 86-111 (2000).
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Catalog No. Product Name/Specifications Size Price
O312 n-OCTYL-β-D-GALACTOPYRANOSIDE, ANAGRADE® 1 g 53.47 [n-Octyl-β-D-galactoside] 5 g 200.50 25 g 751.87
FW 292.4 [40427-75-6] C14H28O6 1 CMC (H2O) ~ 29.5 mM (0.86%)
Purity ≥ 99% by HPLC analysis. PRODUCT INFORMATION Percent alpha < 2 (HPLC) CH OH Percent octanol < 0.005 (HPLC) 2 OH pH (0.5% solution) 5-8 O O (CH2)7CH3 Solubility in water at 0-5°C ≥ 0.5% OH Conductance (0.5% solution) < 10 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 0.5% detergent solution: 340 nm < 0.02 OH 280 nm < 0.04 260 nm < 0.06 225 nm < 0.1 NEW PRODUCTS 1. Stevenson, D. E., Stanley, R. A. and Furneaux, R. H., Biotech. Bioeng. 42, 657-666, (1993).
O311 n-OCTYL-β-D-GLUCOPYRANOSIDE, ANAGRADE® 1 g 29.00 [n-Octyl-β-D-glucoside] 5 g 108.75 25 g 407.81
FW 292.4 [29836-26-8] C14H28O6 1 2 CMC (H2O) ~ 18-20 mM (0.53%) CMC (0.1 M NaCl) ~ 23.4 mM 1 Aggregation Number (H2O) ~ 27-100 CH2OH Purity ≥ 99% by HPLC analysis. O O (CH2)7CH3 For Molar volume check reference 4. OH Percent alpha < 2 (HPLC) Percent octanol < 0.005 (HPLC) HO pH (1% solution) 5-8 OH Solubility in water at 0-5°C ≥ 20% Conductance (10% solution) < 40 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.02 280 nm < 0.04 260 nm < 0.06 225 nm < 0.1 1. Lorber, B., Bishop, J. B. and DeLucas, L. J., Biochim. Biophys. Acta 1023, 254-265 (1990). 2. Chattopadhyay, A. and London, E., Anal. Biochem. 139, 408-412 (1984). 3. Womack, M. D., Kendall, D. A. and MacDonald, R. C., Biochim. Biophys. Acta 733, 210-215 (1983). 4. Brown, G. M., Dubreuil, P., Ichhaporia, F. M. and Desnoyers, J. E., Canadian J. Chem. 48, 2525-2531 (1970). 5. Conlan, S. and Bayley, H., Biochem. 42, 9453-9465 (2003). 6. Fanucci, G. E., Lee, J. Y., and Cafiso, D. S., Biochemistry 42, 13106-13112 (2003). INDEX
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Catalog No. Product Name/Specifications Size Price
O311HA n-OCTYL-α-D-GLUCOPYRANOSIDE, ANAGRADE® 1 g 69.00 [n-Octyl-α-D-glucoside] 5 g 276.00 25 g 1104.00
FW 292.4 [29781-80-4] C14H28O6 1 CMC (H2O) ~ .010 mM (0.0002%) CH2OH Purity ≥ 99% by HPLC analysis. O Percent alpha > 98% (HPLC) OH Percent octanol < 0.005 (HPLC) HO pH (0.1% solution) 5-8 O (CH2)7CH3 Solubility in water at 20°C ≥ 0.1% OH Heating may be required to dissolve detergent Conductance (0.1% solution) < 50 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 0.1% detergent solution: 340 nm < 0.02 PRODUCT INFOMATION 280 nm < 0.04 260 nm < 0.06 225 nm < 0.10 1. Brown, G. M., Dubreuil P., Ichhaporia, F. M., and Deshoyers, J. E., Canadian J. Chem. 48, 2525-2531 (1970).
O311S n-OCTYL-β-D-GLUCOPYRANOSIDE, SOL-GRADE® 1 g 25.73 [n-Octyl-β-D-glucoside] 5 g 96.49
NEW PRODUCTS 25 g 361.83
FW 292.4 [29836-26-8] C14H28O6 1 2 CMC (H2O) ~ 18-20 mM (0.53%) CMC (0.1 M NaCl) ~ 23.4 mM Aggregation Number (H O)1 ~ 27-100 2 CH2OH Purity 97% pure by HPLC analysis. ≥ O O (CH2)7CH3 Percent alpha < 5 (HPLC) OH Percent octanol < 0.05 (HPLC) pH (1% solution) 4-9 HO Solubility in water at 20°C ≥ 20% OH
PRODUCT LISTING Conductance (10% solution) < 500 μS Absorbance of a 1% detergent solution: 340 nm < 0.05 280 nm < 0.1 260 nm < 0.1 225 nm < 0.2 1. See O311 for references.
Anatrace offers five different grades of octyl glucoside and six different grades of dodecyl maltoside. INDEX If you need a special grade of any detergent, ask us.
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Catalog No. Product Name/Specifications Size Price
O311T n-OCTYL-d17-β-D-GLUCOPYRANOSIDE 100 mg 214.25
[n-Octyl-d17-β-D-glucoside, tail deuterated] 250 mg 482.06 500 mg 915.92
FW 309.5 [129522-81-2] C14D17H11O6 1 CMC (H2O) ~ 20 mM
CH OH PRODUCT INFORMATION Purity ≥ 97% pure by HPLC analysis. 2 Percent alpha < 2 (HPLC) O O (CD2)7CD3 Percent octanol < 0.05 (HPLC) OH pH (1% solution) 5-8 HO Solubility in water at 20°C ≥ 10% Conductance (1% solution) < 40 μS OH Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.1 280 nm < 0.15 260 nm < 0.15 225 nm < 0.5 NEW PRODUCTS 1. CMC value for the undeuterated compound.
O311D n-OCTYL-d17-β-D-GLUCOPYRANOSIDE-d7 100 mg 508.66
[n-Octyl-d17-β-D-glucoside-d7] 250 mg 1144.85 500 mg 2060.07
FW 316.5 [869666-57-9] C14D24H4O6 1 CMC (H2O) ~ 19 mM CD2OH D Purity ≥ 97% pure by HPLC analysis. O O (CD2)7CD3 Percent alpha < 2 (HPLC) D OH D Percent octanol < 0.05 (HPLC) HO D Conductance (1% solution) < 40 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 D OH Absorbance of a 1% detergent solution: 340 nm < 0.1 280 nm < 0.15 260 nm < 0.15 225 nm < 0.5 1. CMC value for the undeuterated compound. INDEX
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Catalog No. Product Name/Specifications Size Price
O310 n-OCTYL-β-D-MALTOPYRANOSIDE, ANAGRADE® 1 g 62.05 [n-Octyl-β-D-maltoside] 5 g 248.18 25 g 992.74
FW 454.4 [82494-08-4] C20H38O11 CMC (100 mM NaCl, 20 mM HEPES pH 7.5) ~ 19.5 mM1 (0.89%) Aggregation Number (100 mM NaCl, 20 mM HEPES pH 7.5)1 ~ 47
Purity ≥ 99% by HPLC analysis. CH2OH CH2OH Percent alpha < 2 (HPLC) O O O (CH2)7CH3 Percent octanol < 0.005 (HPLC) OH OH pH (1% solution) 5-8 HO O Solubility in water at 0-5°C ≥ 20% OH Conductance (10% solution) < 40 μS OH Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.02 PRODUCT INFOMATION 280 nm < 0.04 260 nm < 0.06 225 nm < 0.1 1. Anatrace measurement in collaboration with Professor R. M. Garavito (Michigan State University).
O310S n-OCTYL-β-D-MALTOPYRANOSIDE, SOL-GRADE® 1 g 49.64 [n-Octyl-β-D-maltoside] 5 g 198.55
NEW PRODUCTS 25 g 794.19
FW 454.4 [82494-08-4] C20H38O11 CMC (100 mM NaCl, 20 mM HEPES pH 7.5) ~ 19.5 mM1 (0.89%) Aggregation Number (100 mM NaCl, 20 mM HEPES pH 7.5)1 ~ 47
Purity ≥ 98% pure by HPLC analysis. CH2OH CH2OH
Percent alpha < 5 (HPLC) O O O (CH2)7CH3 Percent octanol < 0.05 (HPLC) OH OH pH (1% solution) 4-9 HO O Solubility in water at 20°C ≥ 20% OH PRODUCT LISTING Conductance (10% solution) < 100 μS OH Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.05 280 nm < 0.1 260 nm < 0.1 225 nm < 0.2 1. Anatrace measurement in collaboration with Professor R. M. Garavito (Michigan State University).
Notes: INDEX
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Catalog No. Product Name/Specifications Size Price
O314 n-OCTYL-β-D-THIOGLUCOPYRANOSIDE, ANAGRADE® 1 g 25.16 [n-Octyl-β-D-thioglucoside] 5 g 100.63 25 g 402.52
FW 308.4 [85618-21-9] C14H28O5S CMC (H O) ~ 9 mM1 (0.28%) 2 CH2OH
O PRODUCT INFORMATION Purity ≥ 99% by HPLC analysis. S (CH2)7CH3 Percent alpha < 2 (HPLC) OH pH (0.5% solution) 5-8 HO Solubility in water at 0-5°C ≥ 0.8% OH Conductance (0.5% solution) < 40 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 0.5% detergent solution: 340 nm < 0.1 280 nm < 0.2 260 nm < 0.25 1. Saito, S. and Tsuchiya, T., Biochem. J. 222, 829-832 (1984). 2. Wenk, M. R. and Seelig, J., Biophys. J. 73, 2565-2574 (1997). NEW PRODUCTS
O314LA n-OCTYL-β-D-THIOGLUCOPYRANOSIDE, ANAGRADE® 1 g 34.94 [n-Octyl-β-D-thioglucoside] 5 g 139.78 (Contains almost no alpha isomer) 25 g 559.10
FW 308.4 [85618-21-9] C14H28O5S CMC (H O) ~ 9 mM1 (0.28%) 2 CH2OH O Purity ≥ 99% by HPLC analysis. S (CH2)7CH3 Percent alpha < 0.1 (HPLC) OH pH (0.5% solution) 5-8 HO Solubility in water at 0-5°C ≥ 0.8% OH Conductance (0.5% solution) < 40 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 0.5% detergent solution: 340 nm < 0.1 280 nm < 0.2 260 nm < 0.25 1. See O314 for references.
Notes: INDEX
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Catalog No. Product Name/Specifications Size Price
O320 n-OCTYL-β-D-THIOMALTOPYRANOSIDE, ANAGRADE® 1 g 82.13 [n-Octyl-β-D-thiomaltoside] 5 g 307.99 25 g 1154.93
FW 470.6 [148616-91-5] C20H38O10S CMC (H O) ~ 8.5 mM1 (0.40%) 2 CH2OH CH2OH
Purity ≥ 99% by HPLC analysis. O O S (CH2)7CH3 Percent alpha < 2 (HPLC) OH OH pH (1% solution) 5-8 HO O Solubility in water at 0-5°C ≥ 20% Conductance (10% solution) < 40 μS OH OH Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.05 280 nm < 0.15 260 nm < 0.2 PRODUCT INFOMATION 1. Anatrace measurement. 2. Mechref, Y. and Rassi, Z. E., J. Chromatography 757, 263-273 (1997).
P340 PENTAETHYLENE GLYCOL MONODECYL ETHER, ANAGRADE® 2 ml (1 ampule) 62.84
[C10E5 / Decyl pentaethylene glycol ether / Decylpentaglycol / 10 ml (1 ampule) 251.35 3,6,9,12,15-Pentaoxapentacosan-1-ol] 50 ml (5 ampules) 1005.39
FW 378.55 [23244-49-7] C20H42O6
NEW PRODUCTS 1 CMC (H2O) ~ 0.81mM (0.031%) 2 Aggregation Number (H2O) ~ 73
Supplied as a 50% (w/w) solution under argon. CH3(CH2)9O(CH2CH2O)5H Purity ≥ 99% by HPLC analysis. Purified to contain < 500 μM of equivalent peroxide. pH (1% solution) 5-8 Conductance (10% solution) < 50 μS 1. Borchardt, J. K., TODAY’S CHEMIST AT WORK 5, 10, 20-24, November 1996. 2. LeMaire, M., Champeil, P. and Moller, J. V., Biochimica et Biophysica Acta 1508, 86-111 (2000). PRODUCT LISTING
P350 PENTAETHYLENE GLYCOL MONOOCTYL ETHER, ANAGRADE® 2 ml (1 ampule) 62.84
[C8E5 / Octyl pentaethylene glycol ether / Octylpentaglycol / 10 ml (1 ampule) 251.35 3,6,9,12,15-Pentaoxatricosan-1-ol] 50 ml (5 ampules) 1005.39
FW 350.5 [19327-40-3] C18H38O6 CMC (0.1 M NaCl) ~ 7.1 mM1 (0.25%) Supplied as a 50% (w/w) solution under argon. Purity ≥ 99% by HPLC analysis. CH3(CH2)7O(CH2CH2O)5H Purified to contain < 500 μM of equivalent peroxide. pH (1% solution) 5-8 Conductance (10% solution) < 50 μS 1. Eisele, J. and Vulliez-Le Normand, B., Anal. Biochem. 208, 241-243, (1993). INDEX
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Catalog No. Product Name/Specifications Size Price
P300 PLURONIC® F-68 100 g 11.06 [Polyoxyethylene-polyoxypropylene Block Copolymer / 500 g 44.22 . Methyl-oxirane, polymer with oxirane, (C3H6O C2H4O)x / Poloxamer 188]
FW ~ 8400 [9003-11-6] EO78PO30EO78 1,2 CMC (H2O, 27°C) ~ 17.9 mM 1. Alexandridis, P., Holzwarth, J. F. and Hatton, T. A., Macromolecules 27, 2414-2425 (1994). PRODUCT INFORMATION 2. Alexandridis, P., Athanassiou, V., Fukuda, S. and Hatton, T. A., Langmuir 10, 2604-2612 (1994).
CH3 x ~ 78 HO(CH2CH2O)x (CHCH2O)y (CH2CH2O)z H y ~ 30 z ~ 78
P305 PLURONIC® F-127 100 g 11.06 [Polyoxyethylene-polyoxypropylene Block Copolymer / 500 g 44.22 . Methyl-oxirane, polymer with oxirane, (C3H6O C2H4O)x / Poloxamer 407]
FW ~ 12600 [9003-11-6] EO100PO65EO100 NEW PRODUCTS 1,2 CMC (H2O, 19.5°C) ~ 3.97 mM 1. Alexandridis, P., Holzwarth, J. F. and Hatton, T. A., Macromolecules 27, 2414-2425 (1994). 2. Alexandridis, P., Athanassiou, V., Fukuda, S. and Hatton, T. A., Langmuir 10, 2604-2612 (1994).
CH3 x ~ 100
HO(CH2CH2O)x (CHCH2O)y (CH2CH2O)z H y ~ 65 z ~ 100
P5008 PMAL™-C8 1 g 144.56 [Poly (maleic anhydride-alt-1-decene) substituted with 5 g 578.24 3-(Dimethylamino) propylamine] ( CH CH CH2 CH )n [869856-84-8] (C19H36O3N2)n _ MW ~ 18,500 O C CO2 (CH2)7 Solubility in water at 20oC ≥ 10% CH3 Absorbance of a 0.1% detergent solution: 340 nm < 0.1 NH 280 nm < 0.3
260 nm < 0.5 (CH2)3 + H3C N H
CH3 IR spectrum conforms to specifications. No maleic anhydride present by thermographic analysis 1. Nagy, J. K., Hoffmann, A. K., Keyes, M. H., Gray, D. N., Oxenoid, K. and Sanders, C. R., FEBS Letters 501, 115-120 (2001). 2. Gorzelle, B. M., Hoffmann, A. K., Keyes, M. H., Gray, D. N., Ray, D. G., Sanders, C. R., J. Am. Chem. Soc. 124, Amphipols Can Support the Activity of a Membrane Enzyme, 11594-11595 (2002). INDEX
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Catalog No. Product Name/Specifications Size Price
P5010 PMAL™-C10 1 g 144.56 [Poly (maleic anhydride-alt-1-dodecene) substituted with 5 g 578.24 3-(Dimethylamino) propylamine] ( CH CH CH2 CH )n [869856-88-2] (C H O N ) 21 40 3 2 n _ (CH ) MW ~ 18,000 O C CO2 2 9 o Solubility in water at 20 C ≥ 10% CH3 Absorbance of a 0.1% detergent solution: 340 nm < 0.1 NH 280 nm < 0.3 260 nm < 0.5 (CH2 )3 IR spectrum conforms to specifications. + N No maleic anhydride present by thermographic analysis H3 C H
1. See P5008 for references. CH3
™ PRODUCT INFOMATION P5012 PMAL -C12 1 g 144.56 [Poly (maleic anhydride-alt-1-tetradecene) substituted with 5 g 578.24 3-(Dimethylamino) propylamine] ( CH CH CH2 CH )n [869857-14-7] (C H O N ) 23 44 3 2 n _ (CH ) MW ~ 12,000 O C CO2 2 11 Solubility in water at 20oC 10% ≥ CH3 Absorbance of a 0.1% detergent solution: 340 nm < 0.1 NH 280 nm < 0.3 NEW PRODUCTS 260 nm < 0.5 (CH2)3 IR spectrum conforms to specifications. + No maleic anhydride present by thermographic analysis H3C N H 1. See P5008 for references. CH3
P5016 PMAL™-C16 1 g 144.56 [Poly (maleic anhydride-alt-1-octadecene) substituted with 5 g 578.24 3-(Dimethylamino) propylamine] ( CH CH CH2 CH )n [869857-16-9] (C H O N ) 27 52 3 2 n _
PRODUCT LISTING (CH ) MW ~ 39,000-65,000 O C CO2 2 15 o Solubility in water at 20 C ≥ 1% CH3 Absorbance of a 0.1% detergent solution: 340 nm < 0.1 NH 280 nm < 0.3
260 nm < 0.5 (CH2)3 IR spectrum conforms to specifications. + No maleic anhydride present by thermographic analysis H3C N H 1. See P5008 for references. CH3 INDEX
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Catalog No. Product Name/Specifications Size Price
P310 2-PROPYL-1-PENTYL-β-D-MALTOPYRANOSIDE, ANAGRADE® 1 g 71.76 5 g 287.04
FW 455.5 [869668-28-0] C20H39O11 25 g 1148.16 1 CMC (H2O) ~ 42.5 mM (1.9%) Purity ≥ 99% by HPLC analysis. CH2OH CH2OH PRODUCT INFORMATION Percent alpha < 10% O O O CH2 CH (CH2)2CH3 Percent (2-propyl-1-pentanol) < 0.005% OH OH (CH2)2 pH (1% solution) 5-8 O o HO Solubility in water at 0-5 C ≥ 20% CH3 Conductance (10% solution) < 40 μS OH OH Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.02 280 nm < 0.04 260 nm < 0.06 225 nm < 0.1 1. Anatrace measurement. NEW PRODUCTS
S2000 L-(+)-SELENOMETHIONINE, ANALYTICAL GRADE 250 mg 40.25 [(S)-2-Amino-4-(methylseleno)butyric acid] 1 g 134.16 5 g 536.64
FW 196.11 [3211-76-5] C5H11NO2Se NH2 O Purity ≥ 98% pure by HPLC analysis. 20 CH3 Se (CH2)2 CH C OH Specific Rotation [α] D (C=0.5 in 2N HCl) +18.5° ± 1.5° Melting Point: 258-262° Solubility: Soluble in water, slightly soluble in methanol IR spectrum conforms to specifications.
S3000 SODIUM AZIDE 100 g 30.00 500 g 75.00
FW 65.01 [26628-22-8] NaN3 Form: White crystalline powder Assay: ≥ 99.0% Loss on drying: < 0.5%
References on the use of Selenomethionine : 1. Yang, W., Hendrickson, W. A., Crouch, R. J. and Satow, Y., Structure of Ribonuclease H Phased at 2 A Resolution by MAD Analysis of the Selenomethionyl Protein, Science 249, 1398-1405 (1990). 2. Jornot, L. and Junod, A. F. Differential regulation of glutathione peroxidase by selenomethionine and hyperoxia in endothelial cells, Biochem. J. 306, 581-587 (1995). INDEX 3. Leahy, D. J., Hendrickson, W. A., Aukhil, I. and Erickson, H. P., Structure of a Fibronectin Type III Domain fromTenascin Phased by MAD Analysis of the Selenomethionyl Protein, Science 258, 987-991 (1992). 4. Crane, B. R., Siegel, L. M. and Getzoff, E. D., Sulfite Reductase Structure at 1.6 A: Evolution and Catalysis for Reduction of Inorganic Anions, Science 270, 59-67 (1995).
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Catalog No. Product Name/Specifications Size Price
S1010 SODIUM CHOLATE, ANAGRADE® 5 g 34.69 [3α,7α,12α−Trihydroxy-5β-cholan-24-oic acid, monosodium salt] 25 g 130.09 100 g 416.28 FW 430.6 [361-09-1] C H O Na 24 39 5 CH O 1 2 3 CMC (pH 9.0) ~ 9.5 mM (0.41%) CMC (pH 7.5) ~ 14 mM OH _ + CH3 CH (CH2)2 C O Na 3 Aggregation Number (H2O) ~ 2.0-4.8 CH H Purity ≥ 99% by HPLC analysis. 3 pH (1% solution) 5-8 H Solubility in water at 20°C 40% HO OH ≥ H Absorbance of a 1% detergent solution: 400 nm < 0.02 340 nm < 0.04 280 nm < 0.08 260 nm < 0.1
PRODUCT INFOMATION 1. Brito, R. M. M and Vaz, W. L. C., Anal. Biochem 152, 250-255 (1986). 2. Vendittis, E., Paumbo, G., Parlata, G. and Borchini, U., Anal. Biochem. 115, 278-286 (1981). 3. LeMaire, M., Champeil, P. and Moller, J. V., Biochimica et Biophysica Acta 1508, 86-111 (2000). 4. Iwaki, M., Giotta, L., Akinsiku, A. O., Schagger, H., Fisher, N., Breton, J., and Rich, P. R., Biochem. 42, 11109-11119 (2003).
S1010S SODIUM CHOLATE, SOL-GRADE® 25 g 16.00 [3α,7α,12α−Trihydroxy-5β-cholan-24-oic acid, monosodium salt] 100 g 51.20 500 g 204.80 NEW PRODUCTS FW 430.6 [361-09-1] C24H39O5Na CMC (pH 9.0) ~ 9.5 mM1 (0.41%) CMC (pH 7.5) ~ 14 mM2 (0.60%) 3 Aggregation Number (H2O) ~ 2.0-4.8 O CH3 OH Purity ≥ 98% pure by HPLC analysis. _ + CH3 CH (CH2)2 C O Na pH (1% solution) 5-8 Solubility in water at 20°C ≥ 40% CH3 H Absorbance of a 1% detergent solution: 400 nm < 0.04 H
340 nm < 0.08 HO OH H 280 nm < 0.12
PRODUCT LISTING 260 nm < 0.2 1. See S1010 for references.
S300 SODIUM DODECANOYL SARCOSINE, ANAGRADE® 1 g 33.66 [Sodium lauroyl sarcosine / N-Methyl-N-(1-oxododecyl)-glycine, sodium salt] 5 g 126.23 25 g 473.34
FW 293.4 [137-16-6] C15H29NO3Na 1 CMC (H2O) ~ 14.4 mM (0.42%) O CH3 O Purity ≥ 99% by HPLC analysis. _ Percent lauric acid < 0.1 + CH3(CH2)9CH2 C N CH2 C O Na pH (10% solution) 7-9 Solubility in water at 20°C ≥ 10% Absorbance of a 1% detergent solution: 340 nm < 0.04 280 nm < 0.06 260 nm < 0.08 1. Anatrace measurement. INDEX
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Catalog No. Product Name/Specifications Size Price
S300S SODIUM DODECANOYL SARCOSINE, SOL-GRADE® 5 g 24.99 [Sodium lauroyl sarcosine / N-Methyl-N-(1-oxododecyl)-glycine, sodium salt] 25 g 93.72 100 g 318.63
FW 293.4 [137-16-6] C15H28NNaO3 1 CMC (H2O) ~ 14.4 mM (0.42%)
O CH O PRODUCT INFORMATION Purity ≥ 96% pure by HPLC analysis. 3 Percent lauric acid < 4 _ CH (CH ) CH N CH C O Na+ pH (10% solution) 7-9 3 2 9 2 C 2 Solubility in water at 20°C ≥ 10% Absorbance of a 1% detergent solution: 340 nm < 0.05 280 nm < 0.1 260 nm < 0.15 1. Anatrace measurement.
S1001 SODIUM DODECYL SULFATE, ANAGRADE® 100 g 22.00
[SDS / Sulfuric acid monododecyl ester sodium salt] 500 g 88.00 NEW PRODUCTS 1 kg 140.80
FW 288.38 [151-21-3] C12H26O4SNa 1 2 CMC (pH 7.5) ~ 2.6 mM (0.075%) CMC (H2O) ~ 8.27 mM (0.24%) 3 O Aggregation Number (H2O) ~ 62-101 _ CH3 (CH2 )11 O S O Na + Purity ≥ 99% by HPLC analysis. Absorbance of a 1% detergent solution: 260 nm < 0.04 O 280 nm < 0.02 Chloride < 0.1% Phosphate < 0.0005% Lead < 0.0005% Copper < 0.0005% RNase, DNase activity None detected PRECAUTIONS: Store at room temperature. Protect from moisture. 1. Brito, R. M. M and Vaz, W. L. C., Anal. Biochem 152, 250-255 (1986). 2. Mukerjee, P. and Mysels, K. J., Critical Micelle Concentrations of Aqueous Surfactant Systems, Nat’l. Std. Data Service, National Bureau of Standards, Washington, D.C. (1971). 3. Anatrace Measurement.
S110MT SODIUM (2-SULFONATOETHYL) METHANETHIOSULFONATE 100 mg 33.55 [MTSES / Methanesulfonothioic acid, sodium salt] 500 mg 134.20 1 g 241.56
FW 242.28 [1950-85-2] C3H7NaO5S3 O O
_ + Solubility in water at 0-5°C ≥ 10% CH 3 SS(CH 2 )2 SONa Negatively charged reagent that reacts very rapidly and specifically with cysteine groups. O O Half-life (pH 7.0, 20°C) ~ 370.0 minutes2 1. Akabas, M. H., Stauffer, D. A., Xu, M. and Karlin, A., Science 258, 307-310 (1992). 2. Karlin, A. and Akabas, M. H., in Methods Enzymol. 293, 123-136 (1998).
3. Stauffer, D. A. and Karlin, A., Biochem. 33, 6840-6849 (1994). INDEX 3. Sobszak, I. and Lolkema, J. S., Biochem. 42, 9789-9796 (2003).
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Catalog No. Product Name/Specifications Size Price
S2033 SODIUM TAUROCHOLATE, ANAGRADE® 1 g 31.08 [3α, 7α, 12α-Trihydroxy-5β-cholan-24-oic acid N-(2-sulfoethyl) amide] 5 g 129.48 25 g 453.18
FW 537.7 [145-42-6] C26H44NO7SNa CMC ~ 3-11 mM1 (0.16-0.59%) CH3 Aggregation Number (H O)1 ~ 4 OH 2 CH3 Purity ≥ 95% pure by HPLC analysis. C NHCH 2 CH2 SO3 Na
pH (1% solution) 4-8 CH3 O Solubility in water at 20°C ≥ 10% Absorbance of a 1% detergent solution: 400 nm < 0.02 HO OH 340 nm < 0.04 H 280 nm < 0.08 260 nm < 0.1 1. Black, Shaun D.: http://psyche.uthct.edu/shaun/SBlack/detergnt.html PRODUCT INFOMATION
S350 SUCROSE MONODODECANOATE, ANAGRADE® 1 g 48.14 [β-D-Fructopyranosyl-α-D-glucopyranoside monododecanoate / 5 g 192.57 Lauroyl sucrose / Dodecanoyl sucrose] 25 g 770.27 O FW 524.61 [25339-99-5] C H O 24 44 12 CH (CH ) CH 1 3 2 10 CO 2 CMC (H2O) ~ 0.3 mM (0.016%) O Purity ≥ 97% pure by HPLC analysis. HOCH2 O NEW PRODUCTS pH (1% solution) 5-8 OH OH O OH Solubility in water at 0-5°C ≥ 20% CH2OH OH Conductance (10% solution) < 100 μS OH Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.04 280 nm < 0.08 260 nm < 0.10 NOTE: Sucrose monododecanoate (S350) contains three different isomers which differ in the location of the ester linkage. Esterification takes place at each of the primary alcohols in the sucrose molecule.
PRODUCT LISTING 1. Makino, H., et al, Agric. Biol. Chem. 4, 319 (1983). 2. Ostermeier, C., Harrenga, A., Ermler, U. and Michel, H., Proc. Natl. Acad. Sci. USA 94, 10547-10553 (1997).
T1000 TCEP 5 g 205.00 [Tris (carboxyethyl) phosphine hydrochloride]OH OH 10 g 321.00
O P O FW 286.65 [51805-45-9] C9H15O6P . HCl Form: White powder HCl Assay (titration): ≥ 99% Storage: + 4°C HO O INDEX
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Catalog No. Product Name/Specifications Size Price
T360 n-TETRADECYL-N,N-DIMETHYLAMINE-N-OXIDE, ANAGRADE® 1 g 38.23 [TDAO / N,N-Dimethyl-1-tetradecanamine-N-oxide] 5 g 152.92 25 g 611.69
FW 257.46 [3332-27-2] C16H35NO 1 1 CMC (H2O) ~ 0.29 mM (0.0075%) CMC (0.1 M NaCl) ~ 0.024 mM
Purity ≥ 99% by HPLC analysis. PRODUCT INFORMATION CH3 Chromatographically purified to contain < 600 μM of equivalent peroxide. CH (CH ) N O pH (1% solution) 4-9 3 2 13 Solubility in water at 20oC ≥ 1% CH Conductance (1% solution) < 100 μS 3 Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.02 280 nm < 0.06 260 nm < 0.08 1. Anatrace measurement. NEW PRODUCTS
T305 n-TETRADECYL-N,N-DIMETHYLGLYCINE, ANAGRADE® 1 g 50.96 5 g 203.84
FW 299.4 [2601-33-4] C18H37NO2 25 g 815.36 1 CMC (H2O) ~ 0.034 mM (0.0010%) O CH3 Purity ≥ 99% by HPLC analysis. _ pH (1% solution) 5-8 + O CCH2 N (CH2)13 CH3 Solubility in water at 20°C ≥ 30% Conductance (10% solution) < 100 S μ CH3 Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.05 280 nm < 0.08 260 nm < 0.10 1. Anatrace measurement.
T315 n-TETRADECYL-β-D-MALTOPYRANOSIDE, ANAGRADE® 500 mg 64.85 [n-Tetradecyl-β-D-maltoside / 1 g 116.74 Tetradecyl 4-O-α-D-glucopyranosyl-β-D-glucopyranoside] 5 g 466.96
FW 538.6 [18449-82-6] C26H50O11 1 CH OH CMC (H2O) ~ 0.01 mM (0.00054%) 2 CH2OH Purity ≥ 99% by HPLC analysis. O O O (CH2)13 CH3 Percent alpha < 2 (HPLC) OH OH Percent tetradecanol < 0.005 (HPLC) HO O pH (1% solution) 5-8 OH OH Solubility in water at 0-5°C ≥ 20% Conductance (10% solution) < 40 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10
Absorbance of a 1% detergent solution: 340 nm < 0.04 INDEX 280 nm < 0.06 260 nm < 0.1 225 nm < 0.2 1. Anatrace measurement.
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Catalog No. Product Name/Specifications Size Price
T315S n-TETRADECYL-β-D-MALTOPYRANOSIDE, SOL-GRADE® 500 mg 57.65 [n-Tetradecyl-β-D-maltoside / 1 g 103.76 Tetradecyl 4-O-α-D-glucopyranosyl-β-D-glucopyranoside] 5 g 415.06
FW 538.6 [18449-82-6] C26H50O11 1 CMC (H2O) ~ 0.01 mM (0.00054%) CH2OH CH2OH Purity ≥ 98% pure by HPLC analysis. O O O (CH2)13 CH3 Percent alpha < 5 (HPLC) OH OH Percent tetradecanol < 0.05 (HPLC) HO O pH (1% solution ) 4-9 OH OH Solubility in water at 20°C ≥ 20% Conductance (10% solution) < 100 μS Absorbance of a 1% detergent solution: 340 nm < 0.05 280 nm < 0.1 PRODUCT INFOMATION 260 nm < 0.1 225 nm < 0.2 1. Anatrace measurement.
T355 TETRADECYLTRIMETHYLAMMONIUM CHLORIDE 5 g 20.28 25 g 81.12
FW 279.90 [4574-04-3] C17H38ClN 100 g 259.58 CMC (H O) ~ 0.032 mM1 (0.0009%) NEW PRODUCTS 2 CH3 Aggregation Number (H O)2-3 ~ 62-81 2 + Purity ≥ 95% pure by titration. CH3 N (CH2)13 CH3 pH (1% solution) 5.5-8.5 - CH3 Solubility in water at 20°C ≥ 30% Cl Absorbance of a 1% detergent solution: 340 nm < 0.02 280 nm < 0.04 260 nm < 0.06 225 nm < 0.08 1. Anatrace measurement.
PRODUCT LISTING 2. Neugebauer, J. M., Methods Enzymol. 182, 239-253 (1990). 3. Maillet, J.-B., Lachet, V., and Coveney, P. V., Phys. Chem. Chem. Phys. 1, 5277-5290 (1999).
T350 TETRAETHYLENE GLYCOL MONOOCTYL ETHER, ANAGRADE® 2 ml (1 ampule) 60.42
[C8E4 / Octyl tetraethylene glycol ether / Octyltetraglycol / 10 ml (1 ampule) 241.68 3,6,9,12-Tetraoxaeicosan-1-ol] 50 ml (5 ampules) 966.72
FW 306.45 [19327-39-0] C16H34O5 CMC (0.1M NaCl) ~ 8 mM1 (0.25%) 1 Aggregation Number (H2O) ~ 82 Supplied as a 50% (w/w) aqueous solution under argon.
Purity ≥ 99% by HPLC analysis. CH3(CH2)7O(CH2CH2O)4H Chromatographically purified to contain < 500 μM of equivalent peroxide. pH (5% solution) 5-8
INDEX Conductance (10% solution) < 50 μS 1. LeMaire, M., Champeil, P. and Moller, J. V., Biochimica et Biophysica Acta 1508, 86-111 (2000). 2. Cortes, D. M. and Perozo, E., Biochem. 36, 10343-10352 (1997).
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Catalog No. Product Name/Specifications Size Price
T323 n-TRIDECYL-β-D-MALTOPYRANOSIDE, ANAGRADE® 500 mg 64.85 [n-Tridecyl-β-D-maltoside] 1 g 116.74 5 g 466.96
FW 524.6 [93911-12-7] C25H48O11 1 1 CMC (H2O) ~ 0.033 mM (0.0017%) CMC (0.15 mM NaCl) ~ 0.024 mM (0.0013%) 2
Aggregation Number (100 mM NaCl, 20 mM HEPES pH 7.5) ~ 105 PRODUCT INFORMATION
Purity ≥ 99% by HPLC analysis. CH2OH CH2OH Percent alpha < 2 (HPLC) O O O (CH2)12 CH3 Percent tridecanol < 0.005 (HPLC) OH OH pH (1% solution) 5-8 HO O Solubility in water at 0-5°C ≥ 20% Conductance (10% solution) < 40 μS OH OH Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.04 280 nm < 0.06 260 nm < 0.1 NEW PRODUCTS 225 nm < 0.2 1. Anatrace measurement. 2. Anatrace measurement in collaboration with Professor R. M. Garavito (Michigan State University).
T323LA n-TRIDECYL-β-D-MALTOPYRANOSIDE, ANAGRADE® 500 mg 91.00 [n-Tridecyl-β-D-maltoside] 1 g 160.71 (Contains almost no alpha isomer) 5 g 642.83
FW 524.6 [93911-12-7] C25H48O11 1 1 CMC (H2O) ~ 0.033 mM (0.0017%) CMC (0.15 mM NaCl) ~ 0.024 mM (0.0013%) Aggregation Number (100 mM NaCl, 20 mM HEPES pH 7.5)2 ~ 105
Purity ≥ 99% by HPLC analysis. CH2OH CH2OH
Percent alpha < 0.2 (HPLC) O O O (CH2)12 CH3 Percent tridecanol < 0.005 (HPLC) OH OH pH (1% solution) 5-8 HO O Solubility in water at 0-5°C ≥ 20% Conductance (10% solution) < 40 μS OH OH Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.04 280 nm < 0.06 260 nm < 0.1 225 nm < 0.2 1. Anatrace measurement. 2. Anatrace measurement in collaboration with Professor R. M. Garavito (Michigan State University). INDEX
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Catalog No. Product Name/Specifications Size Price
T323S n-TRIDECYL-β-D-MALTOPYRANOSIDE, SOL-GRADE® 500 mg 57.65 [n-Tridecyl-β-D-maltoside] 1 g 103.76 5 g 415.06
FW 524.6 [93911-12-7] C25H48O11 1 1 CMC (H2O) ~ 0.033 mM (0.0017%) CMC (0.15 mM NaCl) ~ 0.024 mM (0.0013%) Aggregation Number (100 mM NaCl, 20 mM HEPES pH 7.5)2 ~ 105 Purity 97% pure by HPLC analysis. ≥ CH2OH CH2OH Percent alpha < 5 (HPLC) O O O (CH2)12 CH3 Percent tridecanol < 0.05 (HPLC) pH (1% solution) 4-9 OH OH O Solubility in water at 20°C ≥ 20% HO Conductance (10% solution) < 100 μS OH OH Absorbance of a 1% detergent solution: 340 nm < 0.05
PRODUCT INFOMATION 280 nm < 0.1 260 nm < 0.1 225 nm < 0.2
1. Anatrace measurement. 2. Anatrace measurement in collaboration with Professor R. M. Garavito (Michigan State University).
T110MT [2-(TRIMETHYLAMMONIUM)ETHYL] METHANE THIOSULFONATE BROMIDE 100 mg 33.55 [MTSET] 500 mg 134.20 NEW PRODUCTS 1 g 241.56
FW 278.24 [91774-25-3] C6H16BrNO2S2 O _ CH3 Br Solubility in water at 0-5°C 10% ≥ + Positively charged reagent that reacts very rapidly CH3 SS(CH2 )2 N CH3 and specifically with cysteine groups. Half-life (pH 7.0, 20°C) ~ 11.2 minutes1 O CH3 Half-life (pH 6.0, 20°C) ~ 55.0 minutes1 1. Karlin, A. and Akabas, M. H., in Methods Enzymol. 293, 123-136 (1998).
PRODUCT LISTING 2. Stauffer, D. A. and Karlin, A., Biochem. 33, 6840-6849 (1994). 3. Sobszak, I. and Lolkema, J. S., Biochem. 42, 9789-9796 (2003).
T1001 TRITON® X-100 500 ml 26.00 [α-[4-(1,1,3,3-Tetramethylbutyl)phenyl]-ω-hydroxy-poly(oxy-1,2-ethanediyl)]
FW avg. 647 [9002-93-1] t-Oct-C6H4-(OCH2CH2)XOH, x = 9-10 1-4 CMC (H2O) ~ 0.010 - 0.016% (w/v) (0.015%) Aggregation Number (H O)5 ~ 75-165 2 CH3 CH3 Clear viscous liquid CH C CH2 C (OCH CH ) OH Density: 1.070 3 2 2 10 IR spectrum conforms to specifications. CH3 CH3 PRECAUTIONS: Store at room temperature. Protect from moisture. 1. Vendittis, E., Paumbo, G., Parlata, G., and Borchini, U., Anal.Biochem. 115, 278-286 (1981). 2. Ross, S. and Oliver, J. P., J. Phys.Chem. 63, 1671-1674 (1959). 3. Mankovich, A. M., J. Amer. Oil Chem. Soc. 41, 449-452 (1964). INDEX 4. Rosenthal, K. S. and Koussale F., Anal. Chem. 55, 1115-1117 (1983). 5. LeMaire, M., Champeil, P. and Moller, J. V., Biochimica et Biophysica Acta 1508, 86-111 (2000).
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Catalog No. Product Name/Specifications Size Price
T1002 TRITON® X-114 500 ml 26.00 [α-[(1,1,3,3-Tetramethylbutyl)phenyl]-ω-hydroxy-poly(oxy-1-2-ethanediyl)]
FW avg. 536 [9036-19-5] t-Oct-C6H4-(OCH2CH2)XOH, x = 7-8 1 CH3 CH3 CMC (H2O) ~ 0.009% (w/v) (0.011%)
Clear viscous liquid CH3 C CH2 C (OCH2CH2)8OH PRODUCT INFORMATION IR spectrum conforms to specifications. CH3 CH3 PRECAUTIONS: Store at room temperature. Protect from moisture. 1. Rosenthal, K. S. and Koussale F., Anal. Chem. 55, 1115-1117 (1983).
T1003 TWEEN® 20 500 ml 26.00 (Polyoxyethylene(20)sorbitan monolaurate) Poly(oxy-1,2-ethanediyl) derivs., sorbitan monododecanoate HO(H2CH2CO)w (OCH2CH2)xOH FW avg. 1228 [9005-64-5] (OCH2CH2)yOH CMC (H O) ~ 0.059 mM1 (0.0072%) O 2 NEW PRODUCTS
Clear liquid O(CH2CH2O)zR IR spectrum conforms to specifications. Sum of w+x+y+z=20 R=C H C(O) PRECAUTIONS: Store at room temperature. Protect from moisture. 11 23 1. Helenius, A., McCauslin, D. R., Fries, E. and Tanford, C., Methods Enzymol. 56, 743-749 (1979). 2. Wu, G., Kulmacz, R. J., and Tsai, A., Biochemistry 42, 13772-13777 (2003).
T1005 TWEEN® 40 1 gal 120.00 (Polyoxyethylene sorbitan monolaurate) HO(H CH CO) 2 2 w (OCH CH ) OH [9005-66-7] 2 2 x (OCH CH ) OH O 2 2 y Form: Yellow liquid
Storage: Ambient O(CH2CH2O)zR Sum of w+x+y+z=20 R=C15H31C(O)
T1006 TWEEN® 60 1 gal 44.00 (Polyoxyethylene sorbitan monooleate)
[9005-67-8] HO(H2CH2CO)w (OCH2CH2)xOH
(OCH CH ) OH O 2 2 y
O(CH2CH2O)zR Sum of w+x+y+z=20 R=C17H35C(O) INDEX
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Catalog No. Product Name/Specifications Size Price T1004 TWEEN® 80 500 ml 26.00 [Polyoxyethylene(80)sorbitan monolaurate /
Poly(oxy-1,2-ethanediyl) derivs., (Z)-sorbitan mono-9-octadecenoate] HO(H2CH2CO)w (OCH2CH2)xOH FW avg. 1310 [9005-65-6] 1 (OCH2CH2)yOH CMC (H2O) ~ 0.012 mM (0.0016%) O 2 Aggregation Number (H2O) ~ 58 O(CH2CH2O)zR Golden-yellow viscous liquid IR spectrum conforms to specifications. Sum of w+x+y+z=20 R=C17H33C(O) PRECAUTIONS: Store at room temperature. Protect from moisture. 1. Helenius, A., McCauslin, D. R., Fries, E. and Tanford, C., Methods Enzymol. 56, 743-749 (1979). 2. Black, Shaun D.: http://psyche.uthct.edu/shaun/SBlack/detergnt.html
U360 n-UNDECYL-N,N-DIMETHYLAMINE-OXIDE, ANAGRADE® 1 g 50.96 PRODUCT INFOMATION [Undecyldimethylamine-N-oxide / UDAO / N,N-Dimethyl-1-undecamine-N-oxide 5 g 203.84 25 g 815.36
FW 215.38 [15178-71-9] C13H29NO CMC (H O) ~ 3.21 mM1 (0.069%) 2 CH3 Purity ≥ 99% by HPLC analysis. CH3(CH2)10 N O Purified to contain < 500 μM of equivalent peroxide. pH (1% solution) 4-9 CH3
NEW PRODUCTS Solubility in water at 20°C ≥ 30% Conductance (10% solution) < 200 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.02 280 nm < 0.06 260 nm < 0.08 1. Anatrace measurement.
U300 n-UNDECYL-β-D-MALTOPYRANOSIDE, ANAGRADE® 1 g 67.13
PRODUCT LISTING [n-Undecyl-β-D-maltoside] 5 g 268.55 25 g 1074.20
FW 496.6 [253678-67-0] C23H44O11 1 CMC (H2O) ~ 0.59 mM (0.029%) Aggregation Number (100 mM NaCl, 20 mM HEPES pH 7.5)2 ~ 74 CH OH CH OH Purity ≥ 99% by HPLC analysis. 2 2 Percent alpha < 2 (HPLC) O O O (CH2)10 CH3 Percent undecanol < 0.005 (HPLC) OH OH pH (1% solution) 5-8 HO O Solubility in water at 0-5°C ≥ 20% OH OH Conductance (10% solution) < 40 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.02 280 nm < 0.04 260 nm < 0.06 225 nm < 0.1
INDEX 1. Anatrace measurement. 2. Anatrace measurement in collaboration with Professor R. M. Garavito (Michigan State University). 3. Ostermeier, C., Harrenga, A., Ermler, U., and Michel, H., Proc. Natl. Acad. Sci. USA 94, 10547-10553 (1997). 4. Zhang, H., Kurisu, G., Smith, J. L., and Cramer, W. A., PNAS 100, No. 9, 5160-5163 (2003). 98 800-252-1280 • 419-891-3030 • FAX 419-891-3037 • www.anatrace.com Product Listing SERVICE INFORMATION
Catalog No. Product Name/Specifications Size Price
U300HA n-UNDECYL-α-D-MALTOPYRANOSIDE, ANAGRADE® 1 g 118.72 [n-Undecyl-α-D-maltoside / Undecyl maltoside] 5 g 474.88 (Alpha isomer) 25 g 1899.52 FW 496.6 [168037-13-6] C H O 23 44 11 CH2OH CH OH 1 2 CMC (H2O) ~ 0.58 mM (0.029%) O O PRODUCT INFORMATION Purity ≥ 99% by HPLC analysis. OH Percent alpha > 94 (HPLC) OH HO O Percent dodecanol < 0.005 (HPLC) O (CH2)11 CH3 pH (1% solution) 5-8 OH OH Solubility in water at 0-5°C ≥ 20% Conductance (10% solution) < 40 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.05 280 nm < 0.10 260 nm < 0.15
225 nm < 0.25 NEW PRODUCTS 1. Anatrace measurement.
U300LA n-UNDECYL-β-D-MALTOPYRANOSIDE, ANAGRADE® 1 g 92.71 [n-Undecyl-β-D-maltoside] 5 g 370.85 (Low alpha) 25g 1483.41
FW 496.6 [253678-67-0] C23H44O11 1 CMC (H2O) ~ 0.59 mM (0.029%) Aggregation Number (100 mM NaCl, 20 mM HEPES pH 7.5)2 ~ 74
Purity ≥ 99% by HPLC analysis. CH2OH CH2OH Percent alpha < 0.2 (HPLC) O O O (CH2)10 CH3 Percent undecanol < 0.005 (HPLC) OH OH pH (1% solution) 5-8 HO O Solubility in water at 0-5oC ≥ 20% OH OH Conductance (10% solution) < 40 μS Absorbance of a 1% detergent solution: 340 nm < 0.02 280 nm < 0.04 260 nm < 0.06 225 nm < 0.1 1. Anatrace measurement. 2. Anatrace measurement in collaboration with Professor R. M. Garavito (Michigan State University). 3. Ostermeier, C., Harrenga, A., Ermler, U. and Michel, H., Proc. Natl. Acad. Sci. USA 94, 10547-10553 (1997).
Notes: INDEX
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Catalog No. Product Name/Specifications Size Price
U300S n-UNDECYL-β-D-MALTOPYRANOSIDE, SOL-GRADE® 1 g 53.71 [(n-Undecyl-β-D-maltoside] 5 g 214.82 25 g 859.29
FW 496.6 [253678-67-0] C23H44O11 1 CMC (H2O) ~ 0.59 mM (0.029%) Aggregation Number (100 mM NaCl, 20 mM HEPES pH 7.5)2 ~ 74
Purity ≥ 97% pure by HPLC analysis. CH2OH CH2OH Percent alpha < 5 (HPLC) O O O (CH2)10 CH3 Percent undecanol < 0.05 (HPLC) OH OH pH (1% solution) 4-9 O Solubility in water at 20°C ≥ 20% HO Conductance (10% solution) < 100 μS OH OH Absorbance of a 1% detergent solution: 340 nm < 0.05 280 nm < 0.1 PRODUCT INFOMATION 260 nm < 0.1 225 nm < 0.2 1. Anatrace measurement. 2. Anatrace measurement in collaboration with Professor R. M. Garavito (Michigan State University). 3. Ostermeier, C., Harrenga, A., Ermler, U. and Michel, H., Proc. Natl. Acad. Sci. USA 94, 10547-10553 (1997).
U342 n-UNDECYL-β-D-THIOMALTOPYRANOSIDE, ANAGRADE® 1 g 82.13 [n-Undecyl-β-D-thiomaltoside] 5 g 307.99 NEW PRODUCTS 25 g 1154.93
FW 512.7 [148565-57-5] C23H44O10S 1 CMC (H2O) ~ 0.21 mM (0.011%) CH2OH CH2OH
Purity ≥ 98% pure by HPLC analysis. O O S (CH2)10 CH3 Percent alpha < 2 (HPLC) OH OH pH (1% solution) 5-8 HO O Solubility in water at 20°C ≥ 10% OH OH Conductance (10% solution) < 40 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 PRODUCT LISTING Absorbance of a 1% detergent solution: 340 nm < 0.05 280 nm < 0.15 260 nm < 0.2 1. Anatrace measurement. 2. Mechref, Y. and Rassi, Z. E., J. Chromatography 757, 263-273 (1997). INDEX
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Catalog No. Product Name/Specifications Size Price
U310 ω-UNDECYLENYL-β-D-MALTOPYRANOSIDE, ANAGRADE® 1 g 69.68 [ω-Undecylenyl-β-D-maltoside] 5 g 278.72 25 g 1114.88
FW 494.6 [N/A] C23H42O11 1 CMC (H2O) ~ 1.2 mM (0.059%) CH 2 OH CH 2 OH
Purity ≥ 99% by HPLC analysis. PRODUCT INFORMATION O (CH 2 )9 CH CH 2 Percent alpha < 2 (HPLC) O O OH OH Percent ω-undecylenyl alcohol < 0.005 (HPLC) pH (1% solution) 5-8 OH O Solubility in water at 0-5°C ≥ 20% OH OH Conductance (10% solution) < 40 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.02 280 nm < 0.04 260 nm < 0.06 1. Anatrace measurement.
2. Anatrace measurement in collaboration with Professor R. M. Garavito (Michigan State University). NEW PRODUCTS 3. Ostermeier, C., Harrenga, A., Ermler, U. and Michel, H., Proc. Natl. Acad. Sci. USA 94, 10547-10553 (1997).
U1000 UREA, ANAGRADE® 1 kg 47.00
FW 60.06 [57-13-6] NH2CONH2 Form: White crystalline powder O Assay: ≥ 99.5% Identity: By IR Spectral Ratios (260 nm, 6M, 1 cm): < 0.055 H2NNH2 Spectral Ratios (280 nm, 6M, 1 cm): < 0.044 Melting Point: 132-135°C Insoluble Matter < 0.01% Residue on Ignition: < 0.01% Conductivity: < 15 μmhos/cm Cyanate: None detected Chloride (Cl): < 0.0005%
Sulfate (SO4): < 0.001% Copper (Cu): < 0.5 ppm Iron (Fe): < 0.5 ppm Lead (Pb): < 0.5 ppm DNase (endo): Passes test RNase: Passes test Protease: Passes test
B2000 X-GAL, ANAGRADE® H 1 g 50.96 [5-Bromo-4-chloro-3-indoyl-β-D-galactopyranoside] 5 g 203.84 N 25 g 815.36
FW 408.6 [7240-90-6] C14H15BrClNO6 CH OH 2 Br Purity ≥ 99% by HPLC analysis. O
OH O INDEX 20 Cl Specific Rotation[α] D (c=1, H2O:DMF 1:1) -61° ± 3° Solubility > 2% in DMF OH IR spectrum conforms to specifications. OH
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Catalog No. Product Name/Specifications Size Price
B2050 X-GAL-IPTG, Solution 1.5 ml 29.00 Form: Clear, light yellow solution NH HO X-Gal Concentration: ≥ 10 mg/ml HO IPTG Concentration: 8 mg/ml OH S ≥ OH O O O OH OH Br Cl OH To Use: Spread 100 μl X-Gal-IPTG solution on a Petri dish or OH mix 100 μl X-Gal-IPTG solution with 25 ml of molten agar.
B2020 X-GLUC, CHA SALT, ANAGRADE® 25 mg 30.16 [5-Bromo-4-chloro-3-indolyl-β-D-glucuronic acid , H 100 mg 95.68 cyclohexyl ammonium salt] N 500 mg 386.05
PRODUCT INFOMATION FW 521.8 [114162-64-0] C20H26BrClN2O7 COO Br Purity ≥ 99% by HPLC analysis. O O Cl Specific Rotation [ ]20 (c=1, H O: DMF 1:1)-90.5° ± 3° α D 2 OH Solubility 2% in DMF ≥ + IR spectrum conforms to specifications. OH NH3 OH NEW PRODUCTS
B2030 X-GLUC, Na SALT, ANAGRADE® 25 mg 57.20 [5-Bromo-4-chloro-3-indolyl-β-D-glucuronic acid, sodium salt] 100 mg 171.60 500 mg 643.50 H FW 444.6 [129541-41-9] C14H12BrClNO7Na N Purity ≥ 99% by HPLC analysis. _ Specific Rotation [α]20 (C=1, H O: DMF 1:1) -107° ± 3° Na+ PRODUCT LISTING D 2 COO Br Solubility ≥ 2% in DMF O O Cl IR spectrum conforms to specifications. OH OH OH INDEX
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Catalog No. Product Name/Specifications Size Price Kits To select the optimum detergent or combination of detergents and reagents for a particular membrane protein usually requires testing of many detergents. Note also that the detergent which is suitable for extraction may not be useful for storage of the purified protein or for PRODUCT INFORMATION studies conducted on the purified protein. To aid in the selection process, Anatrace offers a wide variety of solid and solution based detergent kits. Listed below are nonionic and ionic Master Detergent Kits which contain one gram of each detergent. Following the master kits are several smaller kits which likewise contain one gram of the specified detergents. Please Note: Kit pricing represents a substantial discount.
D399-NDK NONIONIC MASTER DETERGENT KIT 2489.43 NEW PRODUCTS Contains one gram of each of the detergents listed below:
ANAMEG®-7 n-Hexyl-β-D-maltopyranoside MEGA-8 n-Octyl- -D-maltopyranoside Big CHAP β MEGA-9 n-Nonyl- -D-maltopyranoside Big CHAP, Deoxy β MEGA-10 n-Decyl-α-D-maltopyranoside ™ 2-Propyl-1-pentyl-β-D- CYGENT 1-3 n-Decyl- -D-maltopyranoside β maltopyranoside CYGLU®-3 n-Undecyl-α-D-maltopyranoside CYGLU®-4 n-Undecyl-β-D-maltopyranoside Hexaethylene glycol monooctyl n-Dodecyl-α-D-maltopyranoside ether (C8E6) (50% w/w) ® CYMAL -1 n-Dodecyl-β-D-maltopyranoside Octaethylene glycol monododecyl CYMAL®-2 n-Tridecyl-β-D-maltopyranoside ether (C12E8) (25% w/w) ® CYMAL -3 n-Tetradecyl-β-D-maltopyranoside Pentaethylene glycol monodecyl CYMAL®-4 n-Hexadecyl-β-D-maltopyranoside ether (C10E5) (50% w/w) ® CYMAL -5 ω-Undecylenyl-β-D-maltopyranoside Pentaethylene glycol monooctyl CYMAL®-6 ether (C8E5) (50% w/w) ® n-Heptyl- -D-thioglucopyranoside CYMAL -7 β Tetraethylene glycol monooctyl n-Octyl- -D-thioglucopyranoside β ether (C E ) (50% w/w) 2,6-Dimethyl-4-heptyl-β- n-Nonyl-β-D-thioglucopyranoside 8 4 D-maltopyranoside n-Decyl-β-D-thioglucopyranoside n-Octyl-β-D-galactopyranoside n-Hexyl-β-D-glucopyranoside n-Octyl-β-D-thiomaltopyranoside Sucrose monododecanoate n-Heptyl-β-D-glucopyranoside n-Nonyl-β-D-thiomaltopyranoside Dimethyldecylphosphine oxide n-Octyl-α-D-glucopyranoside n-Decyl-β-D-thiomaltopyranoside Dimethyloctylphosphine oxide n-Octyl-β-D-glucopyranoside n-Undecyl-β-D-thiomaltopyranoside n-Tetradecyl-N,N-dimethylamine- n-Nonyl-β-D-glucopyranoside n-Dodecyl-β-D-thiomaltopyranoside N-oxide (TDAO) n-Decyl-β-D-glucopyranoside n-Dodecyl-N,N-dimethylamine- n-Dodecyl-β-D-glucopyranoside
N-oxide (DDAO) INDEX
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Catalog No. Product Name/Specifications Size Price
D399-IDK IONIC MASTER DETERGENT KIT 2852.93 Contains one gram of each of the detergents listed below:
ANZERGENT®3-8 FOS-CHOLINE®-8 Decyltrimethylammonium chloride ANZERGENT®3-10 FOS-CHOLINE®-9 Dodecyltrimethylammonium chloride ANZERGENT®3-12 FOS-CHOLINE®-10 Hexadecyltrimethylammonium chloride ANZERGENT®3-14 FOS-CHOLINE®-11 Octadecyltrimethylammonium chloride ® C-DODECAFOS™ FOS-CHOLINE -12 Tetradecyltrimethylammonium chloride FOS-CHOLINE®-13 n-Decyl-N,N-dimethylglycine C-OCTAFOS™ FOS-CHOLINE®-14 ® n-Dodecyl-N,N-dimethylglycine 3-Carboxy-ω-heptadecen- FOS-CHOLINE -15 ® n-Tetradecyl-N,N-dimethylglycine amidopropyl dimethylamine FOS-CHOLINE -16 FOS-CHOLINE®-ISO-9 n-Dodecyl-β-iminodipropionic acid, 3-Carboxy-5-pentadecen- FOS-CHOLINE®-ISO-11 disodium salt PRODUCT INFOMATION amidopropyl dimethylamine FOS-CHOLINE®-ISO-11-6U n-Dodecyl-β-iminodipropionic acid, CHAPS FOS-CHOLINE®-UNSAT-11-10 monosodium salt CHAPSO FOS-MEA®-8 Deoxycholic acid, sodium salt CYCLOFOS™-2 FOS-MEA®-10 Sodium cholate CYCLOFOS™-3 FOS-MEA®-12 Sodium dodecanoyl sarcosine ™ Sodium dodecyl sulfate CYCLOFOS -4 FOSFEN™-9 CYCLOFOS™-5 Sodium Taurocholate ™ ™ NOPOL-FOS NEW PRODUCTS CYCLOFOS -6 CYCLOFOS™-7 PMAL™-C8 PMAL™-C10 CYPFOS™-3 PMAL™-C12 PMAL™-C16
D399-CE ALKYL POLYOXYETHYLENE (CXEY) DETERGENT KIT 161.74
Kit contains Hexaethylene glycol monooctyl ether (C8E6) (50% w/w solution), PRODUCT LISTING Octaethylene glycol monododecyl ether (C12E8) (25% w/w solution), Pentaethylene
glycol monodecyl ether (C10E5) (50% w/w solution) and Tetraethylene glycol
monooctyl ether (C8E4) (50% w/w solution) ampuled and stored under Argon. D399-ANZ ANZERGENT® DETERGENT KIT 39.00 Kit contains ANZERGENT® 3-8, 3-10, 3-12 and 3-14. D399-C14 CYMAL® DETERGENT KIT (1-4) 197.91 Kit contains CYMAL®-1, 2, 3 and 4. D399-F812 FOS-CHOLINE® DETERGENT KIT (8-12) 406.98 Kit contains FOS-CHOLINE® 8, 9, 10, 11 and 12. D399-FM812 FOS-MEA® DETERGENT KIT 239.23 Kit contains FOS-MEA®-8, 10 and 12. D399-G GLUCOPYRANOSIDE DETERGENT KIT 191.26 Kit contains n-Hexyl-β-D-glucopyranoside, n-Heptyl-β-D-glucopyranoside, n-Octyl-β-D-glucopyranoside, n-Nonyl-β-D-glucopyranoside, n-Decyl-β-D- glucopyranoside, and n-Dodecyl- -D-glucopyranoside.
INDEX β
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Catalog No. Product Name/Specifications Size Price
D399-HA HIGH ALPHA DETERGENT KIT 213.70 Kit contains n-Decyl-α-D-maltopyranoside, n-Dodecyl-α-D-maltopyranoside, and n-Undecyl-α-D-maltopyranoside. D399-ION IONIC DETERGENT KIT 80.09 Kit contains Sodium cholate, n-Dodecyl-N,N-dimethylglycine, n-Decyl-N,N-
dimethylglycine, Sodium dodecanoyl sarcosine, and Deoxycholic acid, Sodium salt. PRODUCT INFORMATION D399-M611 MALTOPYRANOSIDE DETERGENT KIT (6-11) 181.18 Kit contains n-Hexyl-β-D-maltopyranoside, n-Octyl-β-D-maltopyranoside, n-Nonyl- β-D-maltopyranoside, n-Decyl-β-D-maltopyranoside and n-Undecyl-β-D- maltopyranoside. D399-M1216 MALTOPYRANOSIDE DETERGENT KIT (12-16) 244.73 Kit contains n-Dodecyl-β-D-maltopyranoside, n-Tridecyl-β-D-maltopyranoside, n-Tetradecyl-β-D-maltopyranoside and n-Hexadecyl-β-D-maltopyranoside. D399-MG MEGA DETERGENT KIT 57.21 Kit contains MEGA-8, 9 and 10. NEW PRODUCTS D399-MSF MISCELLANEOUS FOS DETERGENT KIT 327.43 Kit contains FOS-CHOLINE®-ISO-9, NOPOL-FOS™,C-DODECAFOS™ and CYPFOS™-3. D399-MSFU MISCELLANEOUS FOS-CHOLINE® DETERGENT KIT 245.36 Kit contains FOS-CHOLINE®-ISO-11, FOS-CHOLINE®-ISO-11-6U and FOS-CHOLINE®-UNSAT-11-10. D399-OX OXIDE DETERGENT KIT 64.92 Kit contains Dimethyldecylphosphine oxide, n-Tetradecyl-N,N-dimethylamine-N- oxide (TDAO) and n-Dodecyl-N,N-dimethylamine-N-oxide (DDAO). D399-PM816 PMAL™ DETERGENT KIT (8-16) 346.94 Kit contains PMAL™-C8, PMAL™-C10, PMAL™-C12 and PMAL™-C16. D399-POP POPULAR DETERGENT KIT 228.39 Kit contains CHAPS, CYMAL®-5, FOS-CHOLINE®-12, n-Decyl-β-D-maltopyranoside, n-Dodecyl-β-D-maltopyranoside and n-Octyl-β-D-glucopyranoside. D399-QUAT QUATERNARY DETERGENT KIT 29.00 Kit contains Decyltrimethylammonium chloride, Dodecyltrimethylammonium chloride, Hexadecyltrimethylammonium chloride, Octadecyltrimethylammonium chloride, and Tetradecyltrimethylammonium chloride. D399-TG THIOGLUCOPYRANOSIDE DETERGENT KIT 149.96 Kit contains n-Heptyl-β-D-thioglucopyranoside, n-Octyl-β-D-thioglucopyranoside, n-Nonyl-β-D-thioglucopyranoside and n-Decyl-β-D-thioglucopyranoside. D399-TM THIOMALTOPYRANOSIDE DETERGENT KIT 246.39 Kit contains n-Octyl-β-D-thiomaltopyranoside, n-Nonyl-β-D-thiomaltopyranoside, n-Decyl-β-D-thiomaltopyranoside, n-Dodecyl-β-D-thiomaltopyranoside and n-Undecyl-β-D-Thiomaltopyranoside. INDEX
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Catalog No. Product Name/Specifications Size Price Soluble Detergent Kits Save Time and Money Anatrace detergents are now available in kits as ampuled 10% solutions stored under argon. These solution kits cost a fraction of the one gram detergent kits, making the selection of the “right” deter- gent for extraction of a membrane protein less expensive. The master kit listed below contains nearly every detergent offered by Anatrace that possesses sufficient solubility to prepare a 10% solution. DSOL-MK SOLUTION MASTER DETERGENT KIT 1017.00 Contains one ampule (one ml of a 10% solution) of each detergent listed below:
ANAMEG®-7 2,6-Dimethyl-4-heptyl-β-D- n-Undecyl-α-D-maltopyranoside maltopyranoside n-Undecyl-β-D-maltopyranoside PRODUCT INFOMATION ® ANAPOE -20 2-Propyl-1-pentyl maltopyranoside n-Dodecyl-α-D-maltopyranoside ANAPOE®-35 n-Dodecyl-β-D-maltopyranoside ANAPOE®-58 FOS-CHOLINE®-8 n-Tridecyl-β-D-maltopyranoside ANAPOE®-80 FOS-CHOLINE®-9 n-Tetradecyl-β-D-maltopyranoside ANAPOE®-C E FOS-CHOLINE®-10 10 6 n-Heptyl- -D-thioglucopyranoside ® FOS-CHOLINE®-11 β ANAPOE -C10E9 ® FOS-CHOLINE®-12 n-Octyl-β-D-thiomaltopyranoside ANAPOE -C12E8 ® FOS-CHOLINE®-13 n-Nonyl-β-D-thiomaltopyranoside ANAPOE -C12E9 ® FOS-CHOLINE®-14 n-Decyl-β-D-thiomaltopyranoside ANAPOE -C12E10 NEW PRODUCTS ® FOS-CHOLINE®-15 n-Undecyl-β-D-thiomaltopyranoside ANAPOE -C13E8 ANAPOE®-X-100 FOS-CHOLINE®-16 n-Dodecyl-β-D-thiomaltopyranoside ANAPOE®-X-114 FOS-CHOLINE®-ISO-9 ® n-Decyl-N,N-dimethylglycine ANAPOE®-X-305 FOS-CHOLINE -ISO-11 n-Dodecyl-N,N-dimethylglycine ANAPOE®-X-405 FOS-CHOLINE®-ISO-11-6U ® n-Dodecyl- -iminodipropionic acid, ® FOS-CHOLINE -UNSAT-11-10 β ANZERGENT 3-8 disodium salt ® FOS-MEA®-8 ANZERGENT 3-10 n-Dodecyl-β-iminodipropionic acid, ® FOS-MEA®-10 ANZERGENT 3-12 monosodium salt ® ANZERGENT 3-14 FOSFEN™-9 Sodium dodecanoyl sarcosine Big CHAP MEGA-8 PRODUCT LISTING Hexaethylene glycol monooctyl ether (C E ) Big CHAP, deoxy 8 6 ™ Octaethylene glycol monododecyl ether (C E ) NOPOL-FOS 12 8 CHAPS Pentaethylene glycol monodecyl ether (C E ) ™ 10 5 CHAPSO PMAL -C8 Tetraethylene glycol monooctyl ether (C E ) ™ 8 4 Deoxycholic acid, sodium salt PMAL -C10 ™ Sucrose monododecanoate Sodium cholate PMAL -C12 Dimethyldecylphosphine oxide ™ Decyltrimethylammonium chloride C-DODECAFOS n-Tetradecyl-N,N-dimethylamine-N- ™ Dodecyltrimethylammonium chloride CYCLOFOS -2 oxide (TDAO) ™ Hexadecyltrimethylammonium chloride CYCLOFOS -3 n-Dodecyl-N,N-dimethylamine-N- ™ Octadecyltrimethylammonium chloride CYCLOFOS -4 oxide (DDAO) CYCLOFOS™-5 Tetradecyltrimethylammonium chloride CYCLOFOS™-6 n-Hexyl-β-D-glucopyranoside CYCLOFOS™-7 n-Heptyl-β-D-glucopyranoside CYGLU®-3 n-Octyl-β-D-glucopyranoside CYMAL®-1 n-Nonyl-β-D-glucopyranoside CYMAL®-2 n-Hexyl- -D-maltopyranoside CYMAL®-3 β n-Octyl- -D-maltopyranoside CYMAL®-4 β ® n-Nonyl-β-D-maltopyranoside INDEX CYMAL -5 n-Decyl- -D-maltopyranoside CYMAL®-6 α n-Decyl- -D-maltopyranoside CYMAL®-7 β CYPFOS™-3 106 800-252-1280 • 419-891-3030 • FAX 419-891-3037 • www.anatrace.com Kits SERVICE INFORMATION
Catalog No. Product Name/Specifications Size Price
Additional kits containing detergents as 10% aqueous solutions are listed below: DSOL-ANAP1 ANAPOE® MASTER KIT (1 mL size) 30.50 Kit contains 1 mL of all 15 ANAPOE® detergents DSOL-ANP10 ANAPOE® MASTER KIT (10 mL size) 83.00 Kit contains 10 mL of all 15 ANAPOE® detergents PRODUCT INFORMATION ® DSOL-ANPC ANAPOE CyEx KIT (10 mL size) 51.50 ® ® ® Kit contains 10 mL of ANAPOE -35, ANAPOE -58, ANAPOE -C10E6, ® ® ® ® ANAPOE -C10E9, ANAPOE -C12E8, ANAPOE -C12E9, ANAPOE -C12E10, ® and ANAPOE -C13E8. DSOL-ANPT ANAPOE® TRITON/TWEEN KIT (10 mL size) 30.50 Kit contains 10 mL of ANAPOE®-X-100, ANAPOE®-X-114, ANAPOE®-X-305, ANAPOE®-X-405, ANAPOE®-20, and ANAPOE®-80. DSOL-ANZ ANZERGENT® DETERGENT KIT 30.50 Kit contains ANZERGENT® 3-8, 3-10, 3-12 and 3-14.
DSOL-BILE BILE ACID-BASED DETERGENT KIT 62.00 NEW PRODUCTS Kit contains CHAPS, CHAPSO, Sodium cholate, Big CHAP, Big CHAP, deoxy and Deoxycholic acid, sodium salt. DSOL-CY25 CYCLOFOS™ DETERGENT KIT (2-5) 83.00 Kit contains CYCLOFOS™-2, 3, 4 and 5. DSOL-C14 CYMAL® DETERGENT KIT (1-4) 62.00 Kit contains CYMAL®-1, 2, 3 and 4. DSOL-C57 CYMAL® DETERGENT KIT (5-7) 72.50 Kit contains CYMAL®-5, 6 and 7. DSOL-F812 FOS-CHOLINE® DETERGENT KIT (8-12) 72.50 Kit contains FOS-CHOLINE®-8, 9, 10, 11 and 12. DSOL-F1316 FOS-CHOLINE® DETERGENT KIT (13-16) 72.50 Kit contains FOS-CHOLINE®-13, 14, 15 and 16. DSOL-G GLUCOPYRANOSIDE DETERGENT KIT 62.00 Kit contains n-Hexyl−β−D-glucopyranoside, n-Heptyl−β−D-glucopyranoside, n-Octyl−β−D-glucopyranoside and n-Nonyl−β−D-glucopyranoside. DSOL-HA HIGH ALPHA DETERGENT KIT 72.50 Kit contains n-Decyl-α-D-maltopyranoside, n-Dodecyl-α-D-maltopyranoside, and n-Undecyl-α-D-maltopyranoside . DSOL-ION IONIC DETERGENT KIT 41.00 Kit contains Sodium cholate, n-Dodecyl-N,N-dimethylglycine, n-Decyl-N,N- dimethylglyine,Sodium dodecanoyl sarcosine, and Deoxycholic acid, sodium salt. DSOL-M611 MALTOPYRANOSIDE DETERGENT KIT 62.00 Kit contains n-Hexyl−β−D-maltopyranoside, n-Octyl−β−D-maltopyranoside, n-Nonyl−β−D-maltopyranoside, n-Decyl−β−D-maltopyranoside and n-Undecyl−β−D-maltopyranoside. DSOL-M1214 MALTOPYRANOSIDE DETERGENT KIT 62.00 Kit contains n-Dodecyl−β−D-maltopyranoside, n-Tridecyl−β−D-maltopyranoside and n-Tetradecyl−β−D-maltopyranoside. INDEX DSOL-MS MISCELLANEOUS DETERGENT KIT 72.50 Kit contains CHAPS, FOS-CHOLINE®-ISO-9, CHAPSO, DDAO, CYPFOS™-3 and TDAO.
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Catalog No. Product Name/Specifications Size Price
DSOL-MSS MISCELLANEOUS SUGAR-BASED DETERGENT KIT 83.00 Kit contains 2,6 Dimethyl-4-heptyl−β−D-maltopyranoside, ANAMEG®-7, Sucrose monododecanoate, CYGLU®-3, Big CHAP, and Big CHAP, deoxy. DSOL-OX OXIDE DETERGENT KIT 51.50 Kit contains Dimethyldecylphosphine oxide, n-Tetradecyl-N,N-dimethylamine- N-oxide (TDAO) and n-Dodecyl-N,N-dimethylamine-N-oxide (DDAO). DSOL-PM PMAL™ DETERGENT KIT 104.00 Kit contains PMAL™-C8, PMAL™-C10, and PMAL™-C12. DSOL-CE POLYOXYETHYLENE DETERGENT KIT 62.00
Kit contains C8E4, C8E6, C10E5 and C12E8. DSOL-POP POPULAR DETERGENT KIT 72.50 Kit contains CHAPS, CYMAL®-5, n-Decyl−β−D-maltopyranoside, n-Dodecyl−β−D-maltopyranoside, FOS-CHOLINE®-12, and
PRODUCT INFOMATION n-Octyl−β−D-glucopyranoside. DSOL-QUAT QUATERNARY DETERGENT KIT 30.50 Kit contains Decyltrimethylammonium chloride, Dodecyltrimethylammonium chloride, Hexadecyltrimethylammonium chloride, Octadecyltrimethylammonium chloride, and Tetradecyltrimethylammonium chloride. DSOL-TM THIOMALTOPYRANOSIDE DETERGENT KIT 72.50 Kit contains n-Octyl−β−D-Thiomaltopyranoside, n-Nonyl−β−D-Thiomaltopyranoside, n-Decyl−β−D-Thiomaltopyranoside, n-Dodecyl−β−D-Thiomaltopyranoside and NEW PRODUCTS n-Undecyl−β−D-Thiomaltopyranoside. PRODUCT LISTING INDEX
108 800-252-1280 • 419-891-3030 • FAX 419-891-3037 • www.anatrace.com Kits SERVICE INFORMATION
Catalog No. Product Name/Specifications Size Price
P23280 PeroXOquant™ Quantitative Peroxide Assay Kit 1 Kit 128.00 (Pierce Biotechnology, Inc.) Quickly measure peroxide contamination in various biological samples.
PeroXOquant™ Quantitative Peroxide Assays are the simplest The presence of hydrogen peroxide (H2O2) can now be
assays for detecting the presence of peroxides in both aqueous detected to monitor any peroxide contamination that may be PRODUCT INFORMATION and lipid-containing laboratory reagents. The basis of these harmful to biological samples. When performed on a routine 2+ assays is the complexing of ferric ion (Fe ) by H2O2 in the basis, the PeroXOquant™ Quantitative Peroxide Assay presence of xylenol orange. Peroxides in the sample oxidize can prevent inadvertent introduction of peroxides into your Fe2+ to Fe3+, and the Fe3+ will form a colored complex with valuable samples. If the effects of peroxide cannot be avoided xylenol orange that can be read at 560 nm. in a particular system, these assays will help you assess the risk to your sample. Features/Benefits: References: * Fast and easy to use 1. Jiang, Z.-Y., et al. (1991). Lipids 26(10), 853-856. * Peroxidase independent 2. Jiang, Z.-Y., et al. (1992). Anal. Biochem. 202(2), 384-389. * No lipid extraction necessary * Spectrophotometric analysis * No heating required NEW PRODUCTS
Comparison of Assay Protocols for Lipid Peroxide Content PeroXOquant™ Quantitative Peroxide Assay Thiobarbituric Acid Assay
1. Mix one volume of Reagent A with 100 volumes 1. Mix 0.1 ml sample, 0.4 ml H2O and 0.2 ml 7% SDS. of Reagent C to prepare Working Reagent. 2. Stir gently and add 2 ml 0.1 N HCI. 2. Add 950 μl of Working Reagent to 50 μl of sample. 3. Add 0.3 ml 10% phosphotungstic acid. 3. Incubate at room temperature for 30 minutes. 4. Incubate 5 minutes at room temperature. 4. Read at 560 nm (or 595 nm for ELISA plate readers). 5. Add 1 ml 0.67% thiobarbituric acid (TBA) and acetic acid. Total Time: 35 Minutes 6. Heat for 45 minutes at 95°C. 7. Cool in ice bath. 8. Add 5 ml butanol. 9. Vortex and centrifuge for 15 minutes. 10. Determine lipid peroxide concentration in butanol layer by fluorescence at 515 nm excitation and 553 nm emission. Total Time: 80-90 Minutes Ordering Information: Product # Description Pkg Size U.S. Price P23280 PeroXOquant™ Quantitative Kit $128.00 Peroxide Assay Kit Aqueous compatible formulation. Includes: Reagent A (25 mM Ammonium 1 ml Ferrous Sulfate) Reagent B (125 μM Xylenol 2x50 ml Orange in water with Sorbitol) R:35; S:24/25/26,28 Available to our U.S.A. customers only. INDEX
800-252-1280 • 419-891-3030 • FAX 419-891-3037 • www.anatrace.com 109 Technical Data Detergent Solubility It is frustrating when one prepares a detergent solution that precipitates upon cooling to 4oC, often in a time dependent fashion over several days. There are several possible reasons for such precipitation. Combination of reagents causes precipitation Is the pH and ionic strength compatible with each of the components? The presence of an organic solvent as one component can result in the precipitation of some detergents. Try making a solution of each component to test its solubility in the relevant buffer. Microbial growth causes precipitation Many reagents used in protein research are readily biodegradable. For example, alkyl glycoside detergents can easily be degraded by microorganisms, a process which can occur over a period of PRODUCT INFOMATION days and which results in the appearance of a precipitate or suspension. Microbial growth can conveniently be suppressed by including 1 mM EDTA in the buffer or by including a very low concentration of a bacteriocidal agent such as sodium azide (Cat. # S3000). Solutions may also be sterilized by filtering through a 0.2 μm filters. Presence of synthetic precursors Many alkyl glycosides and alkyl maltosides will contain some of the parent alcohol, which may not
NEW PRODUCTS be completely removed during purification. When a concentrated solution of detergent is prepared and cooled to 4oC, a milkiness or cloudiness may become apparent due to precipitation or oil phase formation by the alcohol. This can be avoided by using highly purified detergents which are commercially available, such as ANAGRADE® detergents which contain less than 0.005% (w/w) of the parent alcohol, as compared with levels as high as 0.5% in some commercial preparations of the corresponding detergents. Kinetic effect of detergent solubilization Some detergents require heating for efficient solubilization. For example, when tetradecyl-β-D-
PRODUCT LISTING maltoside is dissolved at a concentration of 20% (w/v) at room temperature and cooled to 4°C, a precipitate appears. However, if the detergent solution is heated to 50°C before cooling, the precipitate does not form. Possibly the tetradecyl-β-D-maltoside forms optically clear colloids at room temperature which reprecipitate when cooled. Thus the detergent was never truly dissolved. This phenomenon has also been observed with HEGA®-10. Supersaturation A concentration of detergent which is fully soluble at room temperature may be supersaturated at 4oC. This is commonly observed for solutions containing sodium dodecyl sulfate. Supersaturated solutions can exist for days or even weeks before precipitation occurs. The only recourse when supersaturation is confirmed, is to reduce the detergent concentration or find an alternate detergent that is more soluble. For example, n-decyl-β-D-glucopyranoside is only slightly soluble in water (~0.1% w/v), but several closely related detergents are quite soluble: n-nonyl-β-D-glucopyranoside, n-decyl-β-D-maltopyranoside, n-nonyl-β-D-thiomaltopyranoside, and n-undecyl- -D-maltopyranoside. Each of these detergents are soluble to 20% (w/v) or INDEX β greater in water.
110 800-252-1280 • 419-891-3030 • FAX 419-891-3037 • www.anatrace.com Technical Data SERVICE INFORMATION Detergent Grades Generally, detergent purity is not as critical for extraction as for protein studies and crystallization processes. When selecting a grade of detergent for extraction and purification, impurities that might degrade or modify proteins should be avoided. Polyoxyethylene detergents which may contain organic peroxides can modify the sulfhydryl groups of proteins. Most of the impurities in the sugar-based nonionic detergents are not reactive and therefore are unlikely to harm the membrane protein being extracted. The impurities of the maltopyranosides PRODUCT INFORMATION and glucopyranosides are generally the corresponding alcohol of the tail portion of the detergent or a detergent with a very similar structure. The presence of alcohol can lead to a cloudy solution, particularly when the protein solution is cool. Since the starting aliphatic material--whether it be the corresponding alcohol, amine, or other functional group--will contain traces of other aliphatic compounds, the detergent will likewise contain traces of detergents with different chain lengths. During extraction, the presence of small amounts of closely related detergents should not present a problem. For physiological studies and for crystallization of membrane proteins the purity of the detergents is much more important. Generally, the highest purity available should be utilized. In most instances, the detergent of choice is a nonionic detergent. The purity should be listed as 99% or greater as measured by a reliable test, such as NEW PRODUCTS HPLC. The conductance should be very low, indicating that there are few ionic impurities. For those nonionic detergents without ultraviolet chromophores, both the ultraviolet absorbance and the fluorescence background should be very low. Related detergents and aliphatic alcohols should be low so as not to be detectable by HPLC or other reliable tests. It is also considered important by some researchers that the detergent be isomerically homogenous. Thus, the aliphatic maltoside and glucoside detergents should be 99% alpha or beta isomer. The following discussion of grades of dodecyl maltoside, one of our most popular detergents, would apply to selecting an appropriate grade of any detergent in our catalog. Three grades of dodecyl maltoside which we offer are D310, D310A and D310S. The grade you purchase depends on your application. D310S and D310A are usually of sufficient purity for extraction and purification of membrane proteins. Indeed, D310S is greater than 98% pure and often is more pure than the dodecyl maltoside you may purchase from other suppliers. D310A is greater than 99% pure but contains up to 15% alpha isomer. D310 is 99% pure or greater and meets many other purity requirements, such as low absorbance in the UV. This grade is generally used when purification steps require high purity detergent or for studies using a purified membrane protein. An additional grade (D310LA) contains less than 0.2% alpha isomer. Some researchers find this grade prefer- able for crystallization studies. The alpha isomer itself is also available (D310HA). For specifications on grades D310LA and D310HA please see pages 51 and 53. If you don't see the particular grade or detergent you need in our catalog, let us know and we can probably prepare it for you.
Please note: The terms maltopyranoside and maltoside are used interchangeably for the same class of compounds. Likewise, glucopyranoside and glucoside refer to the same structure. Alkyl glycoside is a more
general term used for any simple sugar derivative. Thus, all glucosides are glycosides, but not all glycosides are INDEX glucose derivatives or glucosides.
800-252-1280 • 419-891-3030 • FAX 419-891-3037 • www.anatrace.com 111 Technical Data Detergent Stability
The stability of a representative sample of detergents has been tested both in solution and in solid form at Anatrace, Inc. The purpose of this study was to determine guidelines for shipping and storage of solid detergents and to give some indication of their stability in solution.
To determine their stability during shipment, these representative detergents: Dodecyl-β- D-maltoside, CYMAL®-3, HEGA®-10, MEGA-9, Octyl-β-D-glucoside, CHAPS, CHAPSO, Dodecyl-N,N-dimethylglycine, Octyl-β-D-galactoside, Sodium cholate, Dodecyl-N,N-dimethylamine oxide, and FOS-CHOLINE®-10, were stored at 40°C for
PRODUCT INFOMATION one week and room temperature for two weeks. No deterioration was noted after this time when the specifications for each sample were remeasured. That is, all detergent samples remained within specifications. See pages 52, 59 and 81 for specifications for Dodecyl-β-D-maltoside, FOS-CHOLINE®-10 and Octyl-β-D-glucoside.
To obtain an indication of the stability of detergents in solution, ten percent aqueous solu- tions of tetradecyl maltoside, dodecyl maltoside and HEGA®-10 were heated to 55°C for NEW PRODUCTS two hours. Measurement of the percent purity by HPLC, pH, conductance and absor- bance indicated these detergents had not deteriorated during this heat cycle.
It is our goal to supply only the best quality detergents and other reagents. Hence, we will continue to ship detergents to you by an overnight service when possible. We recommend detergents be stored desiccated at -20°C when they will not be used for a month or longer. PRODUCT LISTING INDEX
112 800-252-1280 • 419-891-3030 • FAX 419-891-3037 • www.anatrace.com Technical Data SERVICE INFORMATION Detergent Properties Listed Alphabetically The detergents are arranged in alphabetical order. The conditions used to measure CMC values and aggregation numbers are located in the Alphabetial Product Listing section of the catalog.
Catalog # Page # Detergent Type FW CMC mM (%) Aggregation # A340 20 ANAMEG®-7 N 335.4 19.5 (0.65%) 92
APT020 21 ANAPOE®-20 N 1228 0.059 (0.0072%) PRODUCT INFORMATION APB035 21 ANAPOE®-35 N 1198 0.091 (0.0010%) 40 APB058 21 ANAPOE®-58 N 1122 0.004 (0.00045%) APT080 22 ANAPOE®-80 N 1310 0.012 (0.0016%) 58 ® APO106 22 ANAPOE -C10E6 N 423 0.9 (0.0250%) 40 ® APO109 22 ANAPOE -C10E9 N 555 1.3 (0.053%) ® APO128 23 ANAPOE -C12E8 N 539 0.09 (0.0048%) 123 ® APO129 23 ANAPOE -C12E9 N 583 0.05 (0.003%) ® AP1210 23 ANAPOE -C12E10 N 627 0.2 ® APO138 23 ANAPOE -C13E8 N 553 0.1 (0.0055%) APND40 24 ANAPOE®-NID-P40 N 603 0.05-0.3 100-155 APX100 24 ANAPOE®-X-100 N 647 0.23 (0.015%) 75-165 NEW PRODUCTS APX114 24 ANAPOE®-X-114 N 536 0.2 (0.011%) APX305 25 ANAPOE®-X-305 N 1526 APX405 25 ANAPOE®-X-405 N 1967 0.81 (0.16%) AZ308 25 ANZEGENT™ 3-8 Z 279.6 390 (10.9%) AZ310 26 ANZERGENT® 3-10 Z 307.6 39 (1.2%) 41 AZ312 26 ANZERGENT® 3-12 Z 335.5 2.8 (0.094%) 55-87 AZ314 27 ANZERGENT® 3-14 Z 363.6 0.2 (0.007%) 83-130 B300 27 Big CHAP N 878.1 2.9 (0.25%) 10 B310 28 Big CHAP, deoxy N 862.1 1.4 (0.12%) 8, 16 C640 28 C-DODECAFOS™ Z 349.17 22 (0.77%) C630 29 C-OCTAFOS™ Z 293.13 450 (13%) C314 29 3-Carboxy-ω-heptadecenamidopropyl dimethylamine Z 383.23 0.001 (.00038%) C385 30 3-Carboxy-5-pentadecenamidopropyl Z 368.5 0.19 (0.0070%) dimethylamine C316 30 CHAPS Z 614.9 8 (0.49%) 10 C317 31 CHAPSO Z 630.9 8 (0.50%) 11 C408 31 C-HEGA®-8 N 349.5 277 (9.7%) C409 31 C-HEGA®-9 N 363.5 108 (3.9%) C410 32 C-HEGA®-10 N 377.5 35 (1.3%) C411 32 C-HEGA®-11 N 391.5 11.5 (0.45%) C508 33 CYCLOFOS™-2 Z 293.8 256 (7.5%) C510 34 CYCLOFOS™-3 Z 306.91 43 (1.3%) C512 34 CYCLOFOS™-4 Z 320.92 14 (0.45%) C514 34 CYCLOFOS™-5 Z 334.95 4.5 (0.15%) C516 35 CYCLOFOS™-6 Z 349.17 2.68 (0.094%) C518 35 CYCLOFOS™-7 N 363.3 0.62 (0.022%) C702 35 CYGENT™ 1-3 Z 269.4 500 C323G 36 CYGLU®-3 N 304.4 28 (0.86%) C324G 36 CYGLU®-4 N 318.4 1.8 (0.058%) C321 37 CYMAL®-1 N 438.5 340 (15%) 360 C322 37 CYMAL®-2 N 452.5 120 (5.4%) 104 C324 38 CYMAL®-4 N 480.5 7.6 (0.37%) 45 C325 39 CYMAL®-5 N 494.5 2.4 (0.12%) 66 INDEX C326 40 CYMAL®-6 N 508.5 0.56 (0.028%) 63 C327 41 CYMAL®-7 N 522.5 0.19 (0.0099%) C610 42 CYPFOS™-3 Z 293.3 180 (5.3%) D365 42 n-Decyl-N,N-dimethylamine-N-oxide Z 201.36 10.48 (0.211%)
800-252-1280 • 419-891-3030 • FAX 419-891-3037 • www.anatrace.com 113 Technical Data
Catalog # Page # Detergent Type FW CMC mM (%) Aggregation # D352 43 n-Decyl-N,N-dimethylglycine Z 243.4 19 (0.46%) D321 43 n-Decyl-β-D-glucopyranoside N 320.4 2.2 (0.070%) D322 44 n-Decyl-β-D-maltopyranoside N 482.6 1.8 (0.087%) 69 D322HA 44 n-Decyl-α-D-maltopyranoside N 482.6 N/A D322LA 45 n-Decyl-β-D-maltopyranoside N 482.6 1.8 (0.087%) 69 D323 46 n-Decyl-β-D-thioglucopyranoside N 336.4 0.9 (0.30%) D335 46 n-Decyl-β-D-thiomaltopyranoside N 498.6 0.9 (0.045%) D354 47 Decyltrimethylammonium chloride C 236.0 2.95 (0.07%) D380 47 Deoxycholic acid A 414.6 6 (0.24%) 22
D412 47 DIFOS™-12 Z 532.6 1.18 (0.063%) DH325 48 2,6-Dimethyl-4-heptyl-β-D-maltoside N 468.5 27.5 (1.2%) D330 48 Dimethyldecylphosphine oxide N 218.3 4.66 (0.10%) 131 D328 48 Dimethyloctylphosphine oxide N 190.1 40 (0.76%) 41 D360 49 n-Dodecyl-N,N-dimethylamine-N-oxide Z 229.41 1 (0.023%) 76 D350 50 n-Dodecyl-N,N-dimethylglycine Z 271.4 1.5 (0.041%)
PRODUCT INFOMATION D318 50 n-Dodecyl-β-D-glucopyranoside N 348.5 0.19 (0.0066%) D345 51 n-Dodecyl-β-iminodipropionic acid, A 373.4 N/A disodium salt D346 51 n-Dodecyl-β-iminodipropionic acid, Z 351.5 N/A monosodium salt D310HA 51 n-Dodecyl-α-D-maltopyranoside N 510.6 0.15 (0.0076%) 90 D310 52 n-Dodecyl-β-D-maltopyranoside N 510.6 0.17 (0.0087%) 78-149 D342 54 n-Dodecyl-β-D-thiomaltopyranoside N 526.6 0.05 (0.0026%) D355 55 Dodecyltrimethylammonium Chloride C 263.89 0.046 (0.0012%) 44-61 NEW PRODUCTS F300 57 FOS-CHOLINE®-8 Z 295.4 114 (3.4%) F302 58 FOS-CHOLINE®-9 Z 309.4 39.5 (1.2%) F304 59 FOS-CHOLINE®-10 Z 323.4 11 (0.35%) F306 59 FOS-CHOLINE®-11 Z 337.4 1.85 (0.062%) F308 60 FOS-CHOLINE®-12 Z 351.5 1.5 (0.047%) 50-60 F310 61 FOS-CHOLINE®-13 Z 365.5 0.75 (0.027%) F312 62 FOS-CHOLINE®-14 Z 379.5 0.12 (0.0046%) F312D 62 FOS-CHOLINE®-14 Z 421.5 0.12 (0.0051%) F314 63 FOS-CHOLINE®-15 Z 393.5 0.07 (0.0027%) F316 63 FOS-CHOLINE®-16 Z 407.5 0.013 (0.00053%)
PRODUCT LISTING FCI09 64 FOS-CHOLINE®-ISO-9 Z 309 32 (0.99%) FCI11 64 FOS-CHOLINE®-ISO-11 Z 337.4 26.6 (0.9%) FCI116 65 FOS-CHOLINE®-ISO-11-6U Z 337.16 25.8 (0.87%) FCU110 65 FOS-CHOLINE®-UNSAT-11-10 Z 335.4 6.2 (0.21%) F208 65 FOS-MEA®-8 A 267.01 22 (0.59%) F210 66 FOS-MEA®-10 A 295.01 5.25 (0.15%) F212 66 FOS-MEA®-12 A 323.01 0.43 (0.014%) FF360 66 FOSFEN™-9 Z 385.21 1.35 (0.052%) H108 67 HEGA®-8 N 351.5 109 (3.8%) H109 67 HEGA®-9 N 365.5 39 (1.4%) H110 68 HEGA®-10 N 379.5 7 (0.26%) H111 68 HEGA®-11 N 393.5 1.4 (0.055%) H300 68 n-Heptyl-β-D-glucopyranoside N 278.4 70 (1.9%) H300LA 69 n-Heptyl-β-D-glucopyranoside N 278.4 70 (1.9%) H301 69 n-Heptyl-β-D-thioglucopyranoside N 294.4 29 (0.85%) H301LA 69 n-Heptyl-β-D-thioglucopyranoside N 294.4 29 (0.85%) H320 70 n-Hexadecyl-β-D-maltopyranoside N 566.6 0.0006 (0.00003%) H355 70 Hexadecyltrimethylammonium chloride C 320.0 0.0032 (0.000102%) 61-84 INDEX H350 70 Hexaethylene glycol monooctyl ether N 394.5 10 (0.39%) 32 H310 71 n-Hexyl-β-D-maltopyranoside N 426.4 210 (8.9%) L360S 73 LAPAO Z 300.55 1.56 (0.052%) 126
114 800-252-1280 • 419-891-3030 • FAX 419-891-3037 • www.anatrace.com Technical Data SERVICE INFORMATION
Catalog # Page # Detergent Type FW CMC mM (%) Aggregation # M319 74 MEGA-8 N 321.4 79 (2.5%) M325 75 MEGA-9 N 335.5 25 (0.84%) M320 75 MEGA-10 N 349.5 6 (0.21%) NIDP40 77 Nonidet P40 Substitute N 603 50 (0.05-0.3%) 100-155 N324 77 n-Nonyl-β-D-glucopyranoside N 306.4 6.5 (0.20%) N342LA 82 n-Nonyl-β-D-glucopyranoside N 306.4 6.5 (0.20%) N330 78 n-Nonyl-β-D-maltopyranoside N 468.5 6 (0.28%) 55 PRODUCT INFORMATION N335 79 n-Nonyl-β-D-thioglucopyranoside N 322.4 2.9 (0.093%) N350 79 n-Nonyl-β-D-thiomaltopyranoside N 484.6 3.2 (0.15%) N150 79 NOPOL-FOS™ Z 331.4 42.5 (1.4%) O355 80 Octadecyltrimethylammonium chloride C 348.1 < 0.03 (0.001%) O330 80 Octaethylene glycol monododecyl ether N 538.77 0.09 (0.0048%) 90-120 O312 81 n-Octyl-β-D-galactopyranoside N 292.4 29.5 (0.86%) O311 81 n-Octyl-β-D-glucopyranoside N 292.4 18 (0.53%) 78 O311HA 82 n-Octyl-α-D-glucopyranoside N 292.4 .010 (0.00029%) O310 84 n-Octyl-β-D-maltopyranoside N 454.4 19.5 (0.89%) 47 O314 85 n-Octyl-β-D-thioglucopyranoside N 308.4 9 (0.28%) O314LA 85 n-Octyl-β-D-thioglucopyranoside N 308.4 9 (0.28%)
O320 86 n-Octyl-β-D-thiomaltopyranoside N 470.6 8.5 (0.40%) NEW PRODUCTS P340 86 Pentaethylene glycol monodecyl ether N 378.55 0.81 (0.031%) 73 P350 86 Pentaethylene glycol monooctyl ether N 350.5 7.1 (0.25%) P5008 87 PMAL™-C8 Z N/A P5010 88 PMAL™-C10 Z N/A P5012 88 PMAL™-C12 Z N/A P5016 88 PMAL™-C16 Z N/A P310 89 2-Propyl-1-pentyl maltopyranoside N 455.5 42.5 (1.9%) S1010 90 Sodium cholate A 430.6 9.5 (0.41%) 2-3 S300 90 Sodium dodecanoyl sarcosine A 293.4 14.4 (0.42%) S1001 91 Sodium dodecyl sulfate A 288.38 2.6 (0.075%) 62-101 S2033 92 Sodium taurocholate A 537.7 3-11 (0.16-0.59%) 4 S350 92 Sucrose monododecanoate N 524.61 0.3 (0.016%) T360 93 n-Tetradecyl-N,N-dimethylamine-N-oxide Z 257.46 0.29 (0.0075%) T305 93 n-Tetradecyl-N,N-dimethylglycine Z 299.4 0.034 (0.0010%) T315 93 n-Tetradecyl-β-D-maltopyranoside N 538.6 0.01 (0.00054%) T355 94 Tetradecylmethylammonium chloride C 279.9 0.032 (0.0009%) 62-81 T350 94 Tetraethylene glycol monooctyl ether N 306.45 8 (0.25%) 82 T323 95 n-Tridecyl-β-D-maltopyranoside N 524.6 0.033 (0.0017%) 105 T1001 96 Triton® X-100 N 647 0.23 (0.015%) 75-165 T1002 97 Triton® X-114 N 536 0.2 (0.011%) T1003 97 Tween® 20 N 1228 0.059 (0.0072%) T1004 98 Tween® 80 N 1310 0.012 (0.0016%) 58 U300 98 n-Undecyl-β-D-maltopyranoside N 496.6 0.59 (0.029%) 74 U300HA 99 n-Undecyl-α-D-maltopyranoside N 496.6 0.58 (0.029%) U342 100 n-Undecyl-β-D-thiomaltopyranoside N 512.7 0.21 (0.011%) U360 98 n-Undecyl-N,N-dimethylamine-oxide Z 215.38 3.21 (0.069%) U310 101 ω-Undecylenyl-β-D-maltopyranoside N 494.6 1.2 (0.059%) INDEX
The types of detergents: A = Anionic / C = Cationic / N = Nonionic / Z = Zwitterionic
800-252-1280 • 419-891-3030 • FAX 419-891-3037 • www.anatrace.com 115 Technical Data Detergent Properties Listed by CMC Values The detergents are arranged in the order of their CMC values. The conditions used to measure CMC values and aggregation numbers are located in the Alphabetial Product Listing section of the catalog.
Catalog # Page # Detergent Type FW CMC mM (%) Aggregation # APX305 25 ANAPOE®-X-305 N 1526 N/A D322HA 44 n-Decyl-α-D-maltopyranoside N 482.6 N/A D345 51 Dodecyl-β-iminodipropionic acid, A 373.4 N/A disodium salt D346 51 Dodecyl-β-iminodipropionic acid, Z 351.5 N/A monosodium salt P5008 87 PMAL™-C8 Z N/A P5010 88 PMAL™-C10 Z N/A P5012 88 PMAL™-C12 Z N/A P5016 88 PMAL™-C16 Z N/A
PRODUCT INFOMATION H320 70 n-Hexadecyl-β-D-maltopyranoside N 566.6 0.0006 (0.00003%) C314 29 3-Carboxy-ω-heptadecenamidopropyl dimethylamine Z 383.23 0.001 (0.00038%) H355 70 Hexadecyltrimethylammonium chloride C 320.0 0.0032 (0.000102%) 61-84 APB058 21 ANAPOE®-58 N 1122 0.004 (0.00045%) O311HA 82 n-Octyl-α-D-glucopyranoside N 292.4 .010 (0.00029%) T315 93 n-Tetradecyl-β-D-maltopyranoside N 538.6 0.01 (0.00054%) APT080 22 ANAPOE®-80 N 1310 0.012 (0.0016%) 58 T1004 98 TWEEN® 80 N 1310 0.012 (0.0016%) 58 F316 63 FOS-CHOLINE®-16 Z 407.5 0.013 (0.00053%)
NEW PRODUCTS T1001 96 TRITON® X-100 N 647 0.010-0.016 (0.015%) 75-165 O355 80 Octadecyltrimethylammonium chloride C 348.1 < 0.03 (0.001%) T355 94 Tetradecylmethylammonium chloride C 279 .9 0.032 (0.0009%) 62-81 T323 95 n-Tridecyl-β-D-maltopyranoside N 524.6 0.033 (0.0017%) 105 T323LA 95 n-Tridecyl-β-D-maltopyranoside N 524.6 0.033 (0.0017%) 105 T305 93 n-Tetradecyl-N,N-dimethylglycine Z 299.4 0.034 (0.0010%) D355 55 Dodecyltrimethylammonium Chloride C 263.89 0.046 (0.0012%) 44-61 ® APO129 23 ANAPOE -C12E9 N 583 0.05 (0.003%) D342 54 n-Dodecyl-β-D-thiomaltopyranoside N 526.6 0.05 (0.0026%) APND40 24 ANAPOE®--NID-P40 N 603 0.05-0.3 100-155 ®
PRODUCT LISTING APT020 21 ANAPOE -20 N 1228 0.059 (0.0072%) T1003 97 TWEEN® 20 N 1228 0.059 (0.0072%) F314 63 FOS-CHOLINE®-15 Z 393.5 0.07 (0.0027%) ® APO128 23 ANAPOE -C12E8 N 538.77 0.09 (0.0048%) 123 O330 80 Octaethylene glycol monododecyl ether N 538.77 0.09 (0.0048%) 90 APB035 21 ANAPOE®-35 N 1198 0.091 (00110%) 40 ® APO138 23 ANAPOE -C13E8 N 553 0.1 (0.0055%) F312 62 FOS-CHOLINE®-14 Z 379.5 0.12 (0.0046%) F312D 62 FOS-CHOLINE®-14 Z 421.5 0.12 (0.0051%) D310HA 51 n-Dodecyl-α-D-maltopyranoside N 510.6 0.15 (0.0076%) 90 D310 52 n-Dodecyl-β-D-maltopyranoside N 510.6 0.17 (0.0087%) 78-149 C327 41 CYMAL®-7 N 522.5 0.19 (0.0099%) D318 50 n-Dodecyl-β-D-glucopyranoside N 348.5 0.19 (0.0066%) C385 30 3-Carboxy-5-pentadecenamidopropyl Z 368.5 0.19 (0.0070%) dimethylamine ® AP1210 23 ANAPOE -C12E10 N 627 0.2 AZ314 27 ANZERGENT® 3-14 Z 363.6 0.2 (0.007%) 83-130 APX114 24 ANAPOE®-X-114 N 536 0.2 (0.011%)
INDEX T1002 97 TRITON® X-114 N 536 0.2 (0.011%) U342 100 n-Undecyl-β-D-thiomaltopyranoside N 512.7 0.21 (0.011%) APX100 24 ANAPOE®-X-100 N 647 0.23 (0.015%) 75-165
116 800-252-1280 • 419-891-3030 • FAX 419-891-3037 • www.anatrace.com Technical Data SERVICE INFORMATION
Catalog # Page # Detergent Type FW CMC mM (%) Aggregation # T360 93 n-Tetradecyl-N,N-dimethylamine-N-oxide Z 257.46 0.29 (0.0075%) S350 92 Sucrose monododecanoate N 524.61 0.3 (0.016%) F212 66 FOS-MEA®-12 A 323.01 0.43 (0.014%) C326 40 CYMAL®-6 N 508.5 0.56 (0.028%) 63 C326LA 40 CYMAL®-6 N 508.5 0.56 (0.028%) U300HA 99 n-Undecyl-α-D-maltopyranoside N 496.6 0.58 (0.029%) U300 98 n-Undecyl-β-D-maltopyranoside N 496.6 0.59 (0.029%) 74
C518 35 CYCLOFOS™-7 N 363.3 0.62 (0.022%) PRODUCT INFORMATION F310 61 FOS-CHOLINE®-13 Z 365.5 0.75 (0.027%) P340 86 Pentaethylene glycol monodecyl ether N 378.55 0.81 (0.031%) 73 APX405 25 ANAPOE®-X-405 N 1967 0.81 (0.16%) ® APO106 22 ANAPOE -C10E6 N 423 0.9 (0.025%) 40 D323 46 n-Decyl-β-D-thioglucopyranoside N 336.4 0.9 (0.30%) D335 46 n-Decyl-β-D-thiomaltopyranoside N 498.6 0.9 (0.045%) D360 49 n-Dodecyl-N,N-dimethylamine-N-oxide Z 229.41 1 (0.023%) 76 D412 47 DIFOS™-12 Z 532.6 1.18 (0.063%) U310 101 ω-Undeceylenyl-β-D-maltopyranoside N 494.6 1.2 (0.059%) ® APO109 22 ANAPOE -C10E9 N 555 1.3 (0.053%) FF360 66 FOSFEN™-9 Z 385.21 1.35 (0.052%) NEW PRODUCTS B310 28 Big CHAP, deoxy N 862.1 1.4 (0.12%) 8, 16 H111 68 HEGA®-11 N 393.5 1.4 (0.055%) D350 50 n-Dodecyl-N,N-dimethylglycine Z 271.4 1.5 (0.041%) F308 60 FOS-CHOLINE®-12 Z 351.5 1.5 (0.047%) 50-60 L360S 73 LAPAO Z 300.55 1.56 (0.052%) 126 C324G 36 CYGLU®-4 N 318.4 1.8 (0.058%) D322 44 n-Decyl-β-D-maltopyranoside N 482.6 1.8 (0.087%) 69 D322LA 45 n-Decyl-β-D-maltopyranoside N 482.6 1.8 (0.087%) 69 F306 59 FOS-CHOLINE®-11 Z 337.4 1.85 (0.062%) D321 43 n-Decyl-β-D-glucopyranoside N 320.4 2.2 (0.070%) C325 39 CYMAL®-5 N 494.5 2.4 (0.12%) 66 S1001 91 Sodium dodecyl sulfate A 288.38 2.6 (0.075%) 62-101 C516 35 CYCLOFOS™-6 Z 349.17 2.68 (0.094%) AZ312 26 ANZERGENT® 3-12 Z 335.5 2.8 (0.094%) 55-87 B300 27 Big CHAP N 878.1 2.9 (0.25%) 10 N335 79 n-Nonyl-β-D-thioglucopyranoside N 322.4 2.9 (0.093%) D354 47 Decyltrimethylammonium chloride C 236.0 2.95 (0.07%) S2033 92 Sodium taurocholate A 537.7 3-11 (0.16-0.59%) 4 N350 79 n-Nonyl-β-D-thiomaltopyranoside N 484.6 3.2 (0.15%) U360 98 n-Undecyl-N,N-dimethylamine-oxide Z 215.38 3.21 (0.069%) C514 34 CYCLOFOS™-5 Z 334.95 4.5 (0.15%) D330 48 Dimethyldecylphosphine oxide N 218.3 4.66 (0.10%) 131 F210 66 FOS-MEA®-10 A 295.01 5.25 (0.15%) D380 47 Deoxycholic acid A 414.6 6 (0.24%) 22 M320 75 MEGA-10 N 349.5 6 (0.21%) N330 78 n-Nonyl-β-D-maltopyranoside N 468.5 6 (0.28%) 55 FCU110 65 FOS-CHOLINE®-UNSAT-11-10 Z 335.4 6.2 (0.21%) N324 77 n-Nonyl-β-D-glucopyranoside N 306.4 6.5 (0.20%) N324LA 77 n-Nonyl-β-D-glucopyranoside N 306.4 6.5 (0.20%) H110 68 HEGA®-10 N 379.5 7 (0.26%) P350 86 Pentaethylene glycol monooctyl ether N 350.5 7.1 (0.25%) C324 38 CYMAL®-4 N 480.5 7.6 (0.37%) 45 C316 30 CHAPS Z 614.9 8 (0.49%) 10 INDEX C317 31 CHAPSO Z 630.9 8 (0.50%) 11 T350 94 Tetraethylene glycol monooctyl ether N 306.45 8 (0.25%) 82 O320 86 n-Octyl-β-D-thiomaltopyranoside N 470.6 8.5 (0.40%) O314 85 n-Octyl-β-D-thioglucopyranoside N 308.4 9 (0.28%)
800-252-1280 • 419-891-3030 • FAX 419-891-3037 • www.anatrace.com 117 Technical Data
Catalog # Page # Detergent Type FW CMC mM (%) Aggregation # O314LA 85 n-Octyl-β-D-thioglucopyranoside N 308.4 9 (0.28%) S1010 90 Sodium cholate A 430.6 9.5 (0.41%) 2 H350 70 Hexaethylene glycol monooctyl ether N 394.5 10 (0.39%) 32 D365 42 n-Decyl-N,N-dimethylamine-N-oxide Z 201.36 10.48 (0.211%) F304 59 FOS-CHOLINE®-10 Z 323.4 11 (0.35%) C411 32 C-HEGA®-11 N 391.5 11.5 (0.45%) C512 34 CYCLOFOS™-4 Z 320.92 14 (0.45%) S300 90 Sodium dodecanoyl sarcosine A 293.4 14.4 (0.42%) O311 81 n-Octyl-β-D-glucopyranoside N 292.4 18 (0.53%) 78 D352 43 n-Decyl-N,N-dimethylglycine Z 243.4 19 (0.46%) A340 20 ANAMEG®-7 N 335.4 19.5 (0.65%) 92 O310 84 n-Octyl-β-D-maltopyranoside N 454.4 19.5 (0.89%) 47 C640 28 C-DODECAFOS™ Z 349.17 22.0 (0.77%) F208 65 FOS-MEA®-8 A 267.01 22 (0.59%) M325 75 MEGA-9 N 335.5 25 (0.84%) FCI116 65 FOS-CHOLINE®-ISO-11-6U Z 337.16 25.8 (0.87%) ®
PRODUCT INFOMATION FCI11 64 FOS-CHOLINE -ISO-11 Z 337.4 26.6 (0.9%) DH325 48 2,6-Dimethyl-4-heptyl-β-D-maltoside N 468.5 27.5 (1.2%) C323G 36 CYGLU®-3 N 304.4 28 (0.86%) H301 69 n-Heptyl-β-D-thioglucopyranoside N 294.4 29 (0.85%) H301LA 69 n-Heptyl-β-D-thioglucopyranoside N 294.4 29 (0.85%) O312 81 n-Octyl-β-D-galactopyranoside N 292.4 29.5 (0.86%) FCI09 64 FOS-CHOLINE®-ISO-9 Z 309 32 (0.99%) C323 38 CYMAL®-3 N 466.5 34.5 (1.6%) 38 C410 32 C-HEGA®-10 N 377.5 35 (1.3%) ® NEW PRODUCTS AZ310 26 ANZERGENT 3-10 Z 307.6 39 (1.2%) 41 H109 67 HEGA®-9 N 365.5 39 (1.4%) F302 58 FOS-CHOLINE®-9 Z 309.4 39.5 (1.2%) D328 48 Dimethyloctylphosphine oxide N 190.1 40 (0.76% ) 41 N150 79 NOPOL-FOS™ Z 331.4 42.5 (1.4%) P310 89 2-Propyl-1-pentyl-maltopyranoside N 455.5 42.5 (1.9%) C510 34 CYCLOFOS™-3 Z 306.91 43 (1.3%) NIDP40 77 Nonidet P40 Substitute N 603 50 (0.05-0.3%) 100-155 H300 68 n-Heptyl-β-D-glucopyranoside N 278.4 70 (1.9%) H300LA 69 n-Heptyl-β-D-glucopyranoside N 278.4 70 (1.9%)
PRODUCT LISTING M319 74 MEGA-8 N 321.4 79 (2.5%) C409 31 C-HEGA®-9 N 363.5 108 (3.9%) H108 67 HEGA®-8 N 351.5 109 (3.8%) F300 57 FOS-CHOLINE®-8 Z 295.4 114 (3.4%) C322 37 CYMAL®-2 N 452.5 120 (5.4%) 104 C610 42 CYPFOS™-3 Z 293.3 180 (5.3%) H310 71 n-Hexyl-β-D-maltopyranoside N 426.4 210 (8.9%) H305 71 n-Hexyl-β-D-glucopyranoside N 264.4 250 (6.6%) C508 33 CYCLOFOS™-2 Z 293.8 256 (7.5%) C408 31 C-HEGA®-8 N 349.5 277 (9.7%) C321 37 CYMAL®-1 N 438.5 340 (15%) 360 AZ308 25 ANZERGENT® 3-8 Z 279.6 390 (10.9%) C630 29 C-OCTAFOS™ Z 293.13 450 (13%) C702 35 CYGENT™ 1-3 Z 269.4 500 INDEX
118 800-252-1280 • 419-891-3030 • FAX 419-891-3037 • www.anatrace.com Index SERVICE INFORMATION Catalog Number Index Cat. # Product Name Page # Cat. # Product Name Page # A110MT 2-Aminoethyl methanethiosulfonate hydrobromide 20 C516 CYCLOFOS™-6, ANAGRADE® 35 A340 ANAMEG®-7, ANAGRADE® 20 C518 CYCLOFOS™-7, ANAGRADE® 35 A1000 Ampicillin, sodium salt 20 C610 CYPFOS™-3, ANAGRADE® 42 ® C630 C-OCTAFOS™, ANAGRADE® 29 AP1210 ANAPOE -C12E10 23 APB035 ANAPOE®-35 21 C640 C-DODECAFOS™, ANAGRADE® 28 ® C702 CYGENT™ 1-3, ANAGRADE® 35
APB058 ANAPOE -58 21 PRODUCT INFORMATION APND40 ANAPOE®--NID-P40 24 C3000 CMC dye solution 33 ® C6500 Culture tubes 33 APO106 ANAPOE -C10E6 22 ® CA100 Carbenicillin disodium salt 29 APO109 ANAPOE -C10E9 22 ® CH200 Cholesterol 32 APO128 ANAPOE -C12E8 23 ® CH210 Cholesteryl hemisuccinate tris salt 33 APO129 ANAPOE -C12E9 23 ® D310 n-Dodecyl-β-D-maltopyranoside, ANAGRADE® 52 APO138 ANAPOE -C13E8 23 APT020 ANAPOE®-20 21 D310A n-Dodecyl-β-D-maltopyranoside, ANAGRADE® 52 APT080 ANAPOE®-80 22 D310HA n-Dodecyl-α-D-maltopyranoside, ANAGRADE® 51 APX100 ANAPOE®-X-100 24 D310LA n-Dodecyl-β-D-maltopyranoside, ANAGRADE® 53 APX114 ANAPOE®-X-114 24 D310S n-Dodecyl-β-D-maltopyranoside, SOL-GRADE® 53 ® APX305 ANAPOE -X-305 25 D310T n-Dodecyl-d25-β-D-maltopyranoside 54 APX405 ANAPOE®-X-405 25 D318 n-Dodecyl-β-D-glucopyranoside, ANAGRADE® 50 ® NEW PRODUCTS AZ308 ANZERGENT® 3-8, Analytical Grade 25 D321 n-Decyl-β-D-glucopyranoside, ANAGRADE 43 D322 n-Decyl-β-D-maltopyranoside, ANAGRADE® 44 AZ310 ANZERGENT® 3-10, Analytical Grade 26 D322HA n-Decyl-α-D-maltopyranoside, ANAGRADE® 44 ® AZ312 ANZERGENT 3-12, Analytical Grade 26 D322LA n-Decyl-β-D-maltopyranoside, ANAGRADE® 45 AZ314 ANZERGENT® 3-14, Analytical Grade 27 D322S n-Decyl-β-D-maltopyranoside, SOL-GRADE® 45 B300 Big CHAP, Analytical Grade 27 D323 n-Decyl-β-D-thioglucopyranoside, ANAGRADE® 46 B310 Big CHAP, Deoxy, Analytical Grade 28 D328 Dimethyloctylphosphine oxide 48 B2000 X-GAL, ANAGRADE® 101 D330 Dimethyldecylphosphine oxide, ANAGRADE® 48 B2010 Magenta-GAL 74 D335 n-Decyl-β-D-thiomaltopyranoside, ANAGRADE® 46 B2020 X-Gluc, CHA Salt 102 D342 n-Dodecyl-β-D-thiomaltopyranoside, ANAGRADE® 54 B2030 X-Gluc, Na Salt 102 D345 n-Dodecyl-β-iminodipropionic acid, disodium salt 51 B2040 Blue-GAL 28 D346 n-Dodecyl-β-iminodipropionic acid, monosodium salt 51 B2050 XGAL-IPTG 102 D350 n-Dodecyl-N,N-dimethylglycine, ANAGRADE® 50 C314 3-Carboxy-ω-heptadecenamidopropyl dimethylamine 29 D350S n-Dodecyl-N,N-dimethylglycine, SOL-GRADE® 50 C316 CHAPS, ANAGRADE® 30 D352 n-Decyl-N,N-dimethylglycine, ANAGRADE® 43 C316S CHAPS, SOL-GRADE® 30 D354 Decyltrimethylammonium chloride 47 C317 CHAPSO, ANAGRADE® 31 D355 Dodecyltrimethylammonium chloride 55 C321 CYMAL®-1, ANAGRADE® 37 D360 n-Dodecyl-N,N-dimethylamine-N-oxide, ANAGRADE® 49 C322 CYMAL®-2, ANAGRADE® 37 D360S n-Dodecyl-N,N-dimethylamine-N-oxide,SOL-GRADE® 49 C323 CYMAL®-3, ANAGRADE® 38 D365 n-Decyl-N,N-dimethylamine-N-oxide, ANAGRADE® 42 C323G CYGLU®-3, ANAGRADE® 36 D380 Deoxycholic acid, sodium salt, ANAGRADE® 47 C324 CYMAL®-4, ANAGRADE® 38 D412 DIFOS™-12, ANAGRADE® 47 C324G CYGLU®-4, ANAGRADE® 36 D1000 DTNB 55 C325 CYMAL®-5, ANAGRADE® 39 D2000 DTT, Cleland's Reagent 55 C325S CYMAL®-5, SOL-GRADE® 39 D2000A DTT, Cleland's Reagent, ANAGRADE® 56 C326 CYMAL®-6, ANAGRADE® 40 D2001 DTE 55 C326LA CYMAL®-6, ANAGRADE® 40 DH325 2,6-Dimethyl-4-heptyl-β-D-maltopyranoside, ANAGRADE® 48 C326S CYMAL®-6, SOL-GRADE® 41 E1000 EDTA 56 C327 CYMAL®-7, ANAGRADE® 41 E1001 EGTA 57 C327S CYMAL®-7, SOL-GRADE® 42 E2000 NEM 57 C385 3-Carboxy-5-pentadecenamidopropyl dimethylamine, 30 F208 FOS-MEA®-8, ANAGRADE® 65 ANAGRADE® F210 FOS-MEA®-10, ANAGRADE® 66 C408 C-HEGA®-8, ANAGRADE® 31 F212 FOS-MEA®-12, ANAGRADE® 66 C409 C-HEGA®-9, ANAGRADE® 31 F300 FOS-CHOLINE®-8, ANAGRADE® 57 INDEX C410 C-HEGA®-10, ANAGRADE® 32 F300S FOS-CHOLINE®-8, SOL-GRADE® 58 C411 C-HEGA®-11, ANAGRADE® 32 F302 FOS-CHOLINE®-9, ANAGRADE® 58 C508 CYCLOFOS™-2, ANAGRADE® 33 F302S FOS-CHOLINE®-9, SOL-GRADE® 58 C510 CYCLOFOS™-3, ANAGRADE® 34 F304 FOS-CHOLINE®-10, ANAGRADE® 59 C512 CYCLOFOS™-4, ANAGRADE® 34 F304S FOS-CHOLINE®-10, SOL-GRADE® 59 C514 CYCLOFOS™-5, ANAGRADE® 34 F306 FOS-CHOLINE®-11, ANAGRADE® 59 800-252-1280 • 419-891-3030 • FAX 419-891-3037 • www.anatrace.com 119 Index Catalog Number Index (continued) Cat. # Product Name Page # Cat. # Product Name Page # F306S FOS-CHOLINE®-11, SOL-GRADE® 60 O110MT MTSL 76 F308 FOS-CHOLINE®-12, ANAGRADE® 60 O310 n-Octyl-β-D-maltopyranoside, ANAGRADE® 84 F308D FOS-CHOLINE®-12, Deuterated 60 O310S n-Octyl-β-D-maltopyranoside, SOL-GRADE® 84 F308S FOS-CHOLINE®-12, SOL-GRADE® 61 O311 n-Octyl-β-D-glucopyranoside, ANAGRADE® 81 F310 FOS-CHOLINE®-13, ANAGRADE® 61 O311HA n-Octyl-α-D-glucopyranoside, ANAGRADE® 82 ® ® F310S FOS-CHOLINE -13, SOL-GRADE 61 O311D n-Octyl-d17-β-D-glucopyranoside-d7 83 F312 FOS-CHOLINE®-14, ANAGRADE® 62 O311S n-Octyl-β-D-glucopyranoside, SOL-GRADE® 82 ® F312D FOS-CHOLINE -14, DEUTERATED 62 O311T n-Octyl-d17-β-D-glucopyranoside-d7 83 F312S FOS-CHOLINE®-14, SOL-GRADE® 62 O312 n-Octyl-β-D-galactopyranoside, ANAGRADE® 81 F314 FOS-CHOLINE®-15, ANAGRADE® 63 O314 n-Octyl-β-D-thioglucopyranoside, ANAGRADE® 85 F314S FOS-CHOLINE®-15, ANAGRADE® 63 O314LA n-Octyl-β-D-thioglucopyranoside, ANAGRADE® 85 F316 FOS-CHOLINE®-16, ANAGRADE® 63 O320 n-Octyl-β-D-thiomaltopyranoside, ANAGRADE® 86 F316S FOS-CHOLINE®-16, SOL-GRADE® 64 O330 Octaethylene glycol monododecyl ether, ANAGRADE® 80 FCI09 FOS-CHOLINE®-ISO-9, ANAGRADE® 64 O330A Octaethylene glycol monododecyl ether, Analytical Grade 80 FCI11 FOS-CHOLINE®-ISO-11, ANAGRADE® 64 O355 Octadecyltrimethylammonium chloride 80 FCI116 FOS-CHOLINE®-ISO-11-6U, ANAGRADE® 65 P300 PLURONIC® F-68 87 ® ® ® PRODUCT INFOMATION FCU110 FOS-CHOLINE -UNSAT-11-10, ANAGRADE 65 P305 PLURONIC F-127 87 FF360 FOSFEN™-9, ANAGRADE® 66 P310 2-Propyl-1-pentyl maltopyranoside, ANAGRADE® 89 G1000 Guanidine hydrochloride 67 P340 Pentaethylene glycol monodecyl ether, ANAGRADE® 86 H108 HEGA®-8, ANAGRADE® 67 P350 Pentaethylene glycol monooctyl ether, ANAGRADE® 86 H109 HEGA®-9, ANAGRADE® 67 P5008 PMAL™-C8 87 H110 HEGA®-10, ANAGRADE® 68 P5010 PMAL™-C10 88 H111 HEGA®-11, ANAGRADE® 68 P5012 PMAL™-C12 88 H300 n-Heptyl-β-D-glucopyranoside, ANAGRADE® 68 P5016 PMAL™-C16 88 H300LA n-Heptyl-β-D-glucopyranoside, ANAGRADE® 69 S110MT Sodium (2-sulfonatoethyl) methanethiosulfonate 91 H301 n-Heptyl- -D-thioglucopyranoside, ANAGRADE® 69 S300 Sodium dodecanoyl sarcosine, ANAGRADE® 90 NEW PRODUCTS β H301LA n-Heptyl-β-D-thioglucopyranoside, ANAGRADE® 69 S300S Sodium dodecanoyl sarcosine, SOL-GRADE® 91 H305 n-Hexyl-β-D-glucopyranoside, ANAGRADE® 71 S350 Sucrose monododecanoate, ANAGRADE® 92 H310 n-Hexyl-β-D-maltopyranoside, ANAGRADE® 71 S1001 Sodium dodecyl sulfate, Biotech Grade 91 H320 n-Hexadecyl-β-D-maltopyranoside, ANAGRADE® 70 S1010 Sodium cholate, ANAGRADE® 90 H350 Hexaethylene glycol monooctyl ether, ANAGRADE® 70 S1010S Sodium cholate, SOL-GRADE® 90 H355 Hexadecyltrimethylammonium chloride 70 S2000 L-(+)-Selenomethionine, Analytical Grade 89 I1001 Imidazole 71 S2033 Sodium taurocholate, ANAGRADE® 92 I1002 Isopropyl-β-D-thiogalactopyranoside, ANAGRADE® 72 S3000 Sodium azide 89 I1003 Isopropyl-β-D-thiogalactopyranoside, Analytical 72 T110MT [2-(Trimethylammonium)ethyl] methane thiosulfonate bromide 96 I1003P Isopropyl-β-D-thiogalactopyranoside, Analytical 72 T305 n-Tetradecyl-N,N-dimethylglycine, ANAGRADE® 93 ® ® PRODUCT LISTING L360S LAPAO, SOL-GRADE 73 T315 n-Tetradecyl-β-D-maltopyranoside, ANAGRADE 93 L1000 LB Broth, Ready-Made Powder 73 T315S n-Tetradecyl-β-D-maltopyranoside, SOL-GRADE® 94 L1001 Luria Broth, Ready-Made Powder 74 T323 n-Tridecyl-β-D-maltopyranoside, ANAGRADE® 95 L1002 LDS 73 T323LA n-Tridecyl-β-D-maltopyranoside, ANAGRADE® 95 M110MT MMTS 75 T323S n-Tridecyl-β-D-maltopyranoside, SOL-GRADE® 96 M319 MEGA-8, ANAGRADE® 74 T350 Tetraethylene glycol monooctyl ether, ANAGRADE® 94 M320 MEGA-10, ANAGRADE® 75 T355 Tetradecyltrimethylammonium chloride 94 M325 MEGA-9, ANAGRADE® 75 T360 n-Tetradecyl-N,N-dimethylamine-N-oxide, ANAGRADE® 93 M2500 Micropipettes 75 T1000 TCEP 92 N150 NOPOL-FOS™, ANAGRADE® 79 T1001 TRITON® X-100 96 N324 n-Nonyl-β-D-glucopyranoside, ANAGRADE® 77 T1002 TRITON® X-114 97 N324LA n-Nonyl-β-D-glucopyranoside, ANAGRADE® 77 T1003 TWEEN® 20 97 N324S n-Nonyl-β-D-glucopyranoside, SOL-GRADE® 78 T1004 TWEEN® 80 98 N330 n-Nonyl-β-D-maltopyranoside, ANAGRADE® 78 T1005 TWEEN® 40 97 N335 n-Nonyl-β-D-thioglucopyranoside, ANAGRADE® 79 T1006 TWEEN® 60 97 N350 n-Nonyl-β-D-thiomaltopyranoside, ANAGRADE® 79 U300 n-Undecyl-β-D-maltopyranoside, ANAGRADE® 98 ND195 NDSB-195 76 U300HA n-Undecyl-α-D-maltopyranoside, ANAGRADE® 99 ND201 NDSB-201 76 U300LA n-Undecyl-β-D-maltopyranoside, ANAGRADE® 99 ND211 NDSB-211 76 U300S n-Undecyl-β-D-maltopyranoside, SOL-GRADE® 100 INDEX ND221 NDSB-221 76 U310 ω-Undecylenyl-β-D-maltopyranoside, ANAGRADE® 101 ND256 NDSB-256 76 U342 n-Undecyl-β-D-thiomaltopyranoside, ANAGRADE® 100 NIDP40 Nonidet P40 Substitute 77 U360 n-Undecyl-N,N-dimethylamine-oxide, ANAGRADE® 98 U1000 Urea 101 120 800-252-1280 • 419-891-3030 • FAX 419-891-3037 • www.anatrace.com Index SERVICE INFORMATION INDEX
Product Name/Chemical Name Cat. # Page # Product Name/Chemical Name Cat. # Page # 2-Aminoethyl methanethiosulfonate hydrobromide A110MT 20 CHAPS, ANAGRADE® C316 30 (S)-2-Amino-4-(methylseleno) butyric acid S2000 89 CHAPSO, ANAGRADE® C317 31 Ampicillin, sodium salt A1000 20 C-HEGA®-8, ANAGRADE® C408 31 ANAMEG®-7, ANAGRADE® A340 20 C-HEGA®-9, ANAGRADE® C409 31 ® ® ANAPOE®-20 APT020 21 C-HEGA -10, ANAGRADE C410 32 PRODUCT INFORMATION ® ® ANAPOE®-35 APB035 21 C-HEGA -11, ANAGRADE C411 32 ANAPOE®-58 APB058 21 3-[(3-Cholamidopropyl)dimethylammonio]-2-hydroxy-1- ANAPOE®-80 APT080 22 propanesulfonate C317 31 ® 3-[(3-Cholamidopropyl)-dimethylammonio]-1-propane sulfonate C316 30 ANAPOE -C10E6 APO106 22 ® 5-Cholesten-3β-ol CH200 32 ANAPOE -C10E9 APO109 22 ® Cholesterol CH200 32 ANAPOE -C12E8 APO128 23 ® Cholesteryl hemisuccinate tris salt CH210 33 ANAPOE -C12E9 APO129 23 ANAPOE®-C E AP1210 23 Cleland's Reagent D2000 55 12 10 ® ® Cleland's Reagent, ANAGRADE D2000A 56 ANAPOE -C13E8 APO138 23 ANAPOE®-NID-P40 APND40 24 CMC dye solution C3000 33 ANAPOE®-X-100 APX100 24 Culture tubes C6500 33
ANAPOE®-X-114 APX114 24 Cyclododecyl-1-phosphocholine C640 28 NEW PRODUCTS ™ ® ANAPOE®-X-305 APX305 25 CYCLOFOS -2, ANAGRADE C508 33 ANAPOE®-X-405 APX405 25 CYCLOFOS™-3, ANAGRADE® C510 34 8-Anilino-1-napthalene sulfonic acid C3000 33 CYCLOFOS™-4, ANAGRADE® C512 34 ANZERGENT® 3-8, Analytical Grade AZ308 25 CYCLOFOS™-5, ANAGRADE® C514 34 ™ ® ANZERGENT® 3-10, Analytical Grade AZ310 26 CYCLOFOS -6, ANAGRADE C516 35 CYCLOFOS™-7, ANAGRADE® C518 35 ANZERGENT® 3-12, Analytical Grade AZ312 26 4-Cyclohexyl-1-butyl-β-D-glucoside C324G 36 ANZERGENT® 3-14, Analytical Grade AZ314 27 4-Cyclohexyl-1-butyl-β-D-maltoside C324 38 APO-8 D328 48 4-Cyclohexyl-1-butylphosphocholine C512 34 APO-10 D330 48 2-Cyclohexyl-1-ethyl-β-D-maltoside C322 37 Big CHAP, Analytical Grade B300 27 2-Cyclohexyl-1-ethylphosphocholine C508 33 Big CHAP, Deoxy, Analytical Grade B310 28 7-Cyclohexyl-1-heptyl-β-D-maltoside C327 41 N,N'-bis-(3-D-Gluconamidopropyl) cholamide B300 27 7-Cyclohexyl-1-heptylphosphocholine C518 35 N,N'-bis-(3-D-Gluconamidopropyl) deoxycholamide B310 28 6-Cyclohexyl-1-hexyl-β-D-maltoside C326 40 Blue-GAL B2040 28 6-Cyclohexyl-1- hexylphosphocholine C516 35 ® BRIJ -35 APB035 21 Cyclohexylmethyl-β-D-maltoside C321 37 ® BRIJ -58 APB058 21 5-Cyclohexyl-1-pentyl-β-D-maltoside C325 39 5-Bromo-4-chloro-3-indolyl-β-D-galactopyranoside B2000 101 5-Cyclohexyl-1-pentylphosphocholine C514 34 5-Bromo-6-chloro-3-indolyl-β-D-galactopyranoside B2010 74 3-Cyclohexyl-1-propyl-β-D-glucoside C323G 36 5-Bromo-4-chloro-3-indolyl-β-D-glucuronic acid, 3-Cyclohexyl-1-propyl-β-D-maltoside C323 38 cyclohexyl ammonium salt B2020 102 3-Cyclohexyl-1-propylphosphocholine C510 34 5-Bromo-4-chloro-3-indolyl-β-D-glucuronic Acid, Cyclohexylbutanoyl-N-hydroxyethylglucamide C410 32 sodium salt B2030 102 Cyclohexylethanoyl-N-hydroxyethylglucamide C408 31 5-Bromo-3-indolyl-β-D-galactopyranoside B2040 28 Cyclohexylpentanoyl-N-hydroxyethylglucamide C411 32 ® C8E4, ANAGRADE T350 94 Cyclohexylpropanoyl-N-hydroxyethylglucamide C409 31 ® C8E5, ANAGRADE P350 86 Cyclooctyl-1-phosphocholine C630 29 ® C8E6, ANAGRADE H350 70 3-Cyclopentyl-1-propylphosphocholine C610 42 ® ™ ® C10E5, ANAGRADE P340 86 CYGENT 1-3, ANAGRADE C702 35 ® ® ® C12E8, ANAGRADE O330 80 CYGLU -3, ANAGRADE C323G 36 ® ® C12E8, Analytical Grade O330A 80 CYGLU -4, ANAGRADE C324G 36 ® ® C12E23 APB035 21 CYMAL -1, ANAGRADE C321 37 ® ® C16E20 APB058 21 CYMAL -2, ANAGRADE C322 37 ™ ® C-DODECAFOS , ANAGRADE C640 28 ® ® CYMAL -3, ANAGRADE C323 38 INDEX ™ ® C-OCTAFOS , ANAGRADE C630 29 CYMAL®-4, ANAGRADE® C324 38 Carbenicillin disodium salt CA100 29 CYMAL®-5, ANAGRADE® C325 39 α-Carboxybenzylpenicillin CA100 29 CYMAL®-5, SOL-GRADE® C325S 39 3-Carboxy-ω-heptadecenamidopropyl dimethylamine C314 29 CYMAL®-6, ANAGRADE® C326 40 3-Carboxy-5-pentadecenamidopropyl dimethylamine C385 30 CYMAL®-6, ANAGRADE® C326LA 40
800-252-1280 • 419-891-3030 • FAX 419-891-3037 • www.anatrace.com 121 Index INDEX (continued)
Product Name/Chemical Name Cat. # Page # Product Name/Chemical Name Cat. # Page # CYMAL®-6, SOL-GRADE® C326S 41 N,N-Dimethyl-N-(3-sulfopropyl)-1-decanaminium CYMAL®-7, ANAGRADE® C327 41 hydroxide, inner salt AZ310 26 CYMAL®-7, SOL-GRADE® C327S 42 N,N-Dimethyl-1-N-(3-sulfopropyl)-1-dodecanaminium CYPFOS™-3, ANAGRADE® C610 42 hydroxide, inner salt AZ312 26 DDAO D360 49 N,N-Dimethyl-N-(3-sulfopropyl)-1-octaminium DDAO D360S 49 hydroxide, inner salt AZ308 25 Decanoyl-N-hydroxyethylglucamide H110 68 Dimethyl(3-sulfopropyl)tetradecyl-ammonium Decanoyl-N-methylglucamide M320 75 hydroxide, inner salt AZ314 27 3,6,9,12,15,18,24,27,30-Decaoxadotetracontan-1-ol AP1210 23 N,N-Dimethyl-3-sulfo-N-[3-[[(3α,5β,7α,12α)-3,7,12-trihydroxy- n-Decyl-N,N-dimethylamine-N-oxide, ANAGRADE® D365 42 24-oxocholan-24-yl]amino]propyl]-1-propanaminium n-Decyl-N,N-dimethyl-3-ammonio-1-propanesulfonate AZ310 26 hydroxide, inner salt C316 30 n-Decyl-N,N-dimethylglycine, ANAGRADE® D352 43 N,N-Dimethyl-1-tetradecanamine, N-oxide T360 93 Decyldimethyl-phosphine oxide D330 48 N,N-Dimethyl-1-undecamine-N-oxide, ANAGRADE® U360 98 n-Decyl-β-D-glucopyranoside, ANAGRADE® D321 43 Disodium-N-lauryl-β-iminodipropionate D345 51 PRODUCT INFOMATION n-Decyl-β-D-glucoside D321 43 5,5'-Dithiobis-(2-nitrobenzoic) acid (DTNB) D1000 55 α-Decyl-ω-hydroxy-poly(oxy-1,2-ethanediyl) APO109 22 Dithioerythritol D2001 55 n-Decyl-β-D-maltopyranoside, ANAGRADE® D322 44 1,4-Dithiothreitol D2000 55 n-Decyl-α-D-maltopyranoside, ANAGRADE® D322HA 44 1,4-Dithiothreitol D2000A 56 n-Decyl-β-D-maltopyranoside, ANAGRADE® D322LA 45 Dodecanoyl sucrose S350 92 n-Decyl-β-D-maltopyranoside, SOL-GRADE® D322S 45 3-Dodecylamido-N,N'-dimethylpropyl aminoxide L360S 73 n-Decyl-β-D-maltoside, ANAGRADE® D322 44 α-Dodecyl-ω-hydroxy-poly(oxy-1,2-ethanediyl) APO129 23 n-Decyl-α-D-maltoside, ANAGRADE® D322HA 44 α-Dodecyl-ω-hydroxy-poly(oxy-1,2-ethanediyl) APB035 21 n-Decyl-β-D-maltoside, ANAGRADE® D322LA 45 n-Dodecyl-N,N-dimethyl-3-ammonio-1-propanesulfonate AZ312 26
NEW PRODUCTS n-Decyl-β-D-maltoside, SOL-GRADE® D322S 45 n-Dodecyl-N,N-dimethylamine-N-oxide, ANAGRADE® D360 49 Decyl pentaethylene glycol ether P340 86 n-Dodecyl-N,N-dimethylamine-N-oxide, SOL-GRADE® D360S 49 Decylpentaglycol P340 86 n-Dodecyl-N,N-dimethylglycine, ANAGRADE® D350 50 n-Decyl-β-D-thioglucopyranoside, ANAGRADE® D323 46 n-Dodecyl-N,N-dimethylglycine, SOL-GRADE® D350S 50 n-Decyl-β-D-thioglucoside D323 46 Dodecyl-1,2-diphosphocholine D412 47 n-Decyl-β-D-thiomaltopyranoside, ANAGRADE® D335 46 n-Dodecyl-β-D-glucopyranoside, ANAGRADE® D318 50 n-Decyl-β-D-thiomaltoside D335 46 Dodecyl 4-O-α-D-glucopyranosyl-β-D-glucopyranoside D310 52 Decylphospho-n-methylethanolamine F210 66 n-Dodecyl-β-D-glucoside D318 50 n-Decylphosphocholine, ANAGRADE® F304 59 α-Dodecyl-ω-hydroxy-poly(oxy-1,2-ethanediyl) APB035 21 n-Decylphosphocholine, SOL-GRADE® F304S 59 n-Dodecyl-β-iminodipropionic acid, disodium salt D345 51 Decyltrimethylammonium chloride D354 47 n-Dodecyl-β-iminodipropionic acid, monosodium salt D346 51 PRODUCT LISTING Deoxycholic acid, sodium salt, ANAGRADE® D380 47 n-Dodecyl-α-D-maltopyranoside, ANAGRADE® D310HA 51 DIFOS™-12, ANAGRADE® D412 47 n-Dodecyl-β-D-maltopyranoside, ANAGRADE® D310 52 3α,12α-Dihydroxy-5β-cholan-24-oic acid, monosodium salt D380 47 n-Dodecyl-β-D-maltopyranoside, ANAGRADE® D310A 52 Dimethylbenzlammoniumpropanesulfonate ND256 76 n-Dodecyl-β-D-maltopyranoside, ANAGRADE® D310LA 53 N,N-Dimethyl-(3-carboxy-pentadec-5-ene)amidopropylamine, n-Dodecyl-β-D-maltopyranoside, SOL-GRADE® D310S 53 ANAGRADE® C385 30 n-Dodecyl-d25-β-D-maltopyranoside D310T 54 Dimethylethylammoniumpropanesulfonate ND195 76 n-Dodecyl-α-D-maltoside, ANAGRADE® D310HA 51 N,N-Dimethyl-(3-carboxy-heptadec-16-ene)amidopropylamine C314 29 n-Dodecyl-β-D-maltoside, ANAGRADE® D310 52 2,6-Dimethyl-4-heptyl-β-D-maltopyranoside, ANAGRADE® DH325 48 n-Dodecyl-β-D-maltoside, ANAGRADE® D310A 52 2,6-Dimethyl-4-heptylphosphocholine FCI09 64 n-Dodecyl-β-D-maltoside, ANAGRADE® D310LA 53 N,N-Dimethyl-3(4-methyl-1-carboxy-2-cyclohexylamido) n-Dodecyl-β-D-maltoside, SOL-GRADE® D310S 53 propylamine C702 35 n-Dodecyl-d25-β-D-maltoside D310T 54 N,N-Dimethyl-3(4-methyl-2-carboxy-1-cyclohexylamido) Dodecyl octaethylene glycol ether O330 80 propylamine C702 35 Dodecyl octaglycol O330 80 2,8-Dimethyl-5-nonylphosphocholine FCI11 64 n-Dodecylphosphocholine, ANAGRADE® F308 60 6,6-Dimethylbicyclo[3.1.1]hept-2-ene-2-ethylphosphocholine N150 79 n-Dodecylphosphocholine, SOL-GRADE® F308S 61 Dimethyldecylphosphine oxide, ANAGRADE® D330 48 Dodecylphospho-n-methylethanolamine F212 66 Dimethyl(2-hydroxyethyl)ammonium-1-propanesulfonate, 98% ND211 76 n-Dodecyl-β-D-thiomaltopyranoside, ANAGRADE® D342 54 INDEX Dimethyloctylphosphine, oxide, ANAGRADE® D328 48 n-Dodecyl-β-D-thiomaltoside D342 54 N,N-Dimethyl-1-dodecanamine, N-oxide D360 49 Dodecyltrimethylammonium chloride D355 55
122 800-252-1280 • 419-891-3030 • FAX 419-891-3037 • www.anatrace.com Index SERVICE INFORMATION INDEX (continued)
Product Name/Chemical Name Cat. # Page # Product Name/Chemical Name Cat. # Page # DTE D2001 55 n-Hexadecyl-β-D-maltopyranoside, ANAGRADE® H320 70 DTNB D1000 55 n-Hexadecyl-β-D-maltoside H320 70 DTT, Cleland's Reagent D2000 55 2-[[(Hexadecyloxy)hydroxyphosphinyl]oxy]-N,N,N-trimethyl- DTT, Cleland's Reagent, ANAGRADE® D2000A 56 ethanaminium hydroxide, inner salt F316 63 ® EDTA E1000 56 n-Hexadecylphosphocholine, ANAGRADE F316 63 PRODUCT INFORMATION EGTA E1001 57 n-Hexadecylphosphocholine, SOL-GRADE® F316S 64 Empigen BB® D350 50 Hexadecyltrimethylammonium chloride H355 70 Ethylene diaminetetracetic acid, disodium salt, dihydrate E1000 56 n-Hexaethylene glycol monooctyl ether, ANAGRADE® H350 70 Ethylene glycol-O,O'-bis(2-aminoethyl-N,N,N',N'-tetracetic acid) E1001 57 3,6,9,12,15,18-Hexaoxaoctacosan-1-ol APO106 22 N-Ethylmaleimide (NEM) E2000 57 n-Hexyl-β-D-glucopyranoside, ANAGRADE® H305 71 FOS-CHOLINE®-8, ANAGRADE® F300 57 n-Hexyl-β-D-glucoside H305 71 FOS-CHOLINE®-8, SOL-GRADE® F300S 58 n-Hexyl-β-D-maltopyranoside, ANAGRADE® H310 71 FOS-CHOLINE®-9, ANAGRADE® F302 58 n-Hexyl-β-D-maltoside H310 71 FOS-CHOLINE®-9, SOL-GRADE® F302S 58 3β-Hydroxy-5-cholestene CH200 32 FOS-CHOLINE®-10, ANAGRADE® F304 59 Igepal CA-630 APND40 24 FOS-CHOLINE®-10, SOL-GRADE® F304S 59 Igepal CA-630 NIDP40 77 ® ® FOS-CHOLINE -11, ANAGRADE F306 59 Imidazole I1001 71 NEW PRODUCTS FOS-CHOLINE®-11, SOL-GRADE® F306S 60 IPTG, ANAGRADE® I1002 72 FOS-CHOLINE®-12, ANAGRADE® F308 60 IPTG, Analytical Grade I1003 72 FOS-CHOLINE®-12, DEUTERATED F308D 60 IPTG, Analytical Grade I1003P 72 FOS-CHOLINE®-12, SOL-GRADE® F308S 61 Isopropyl-β-D-thiogalactopyranoside, ANAGRADE® I1002 72 FOS-CHOLINE®-13, ANAGRADE® F310 61 Isopropyl-β-D-thiogalactopyranoside, Analytical Grade I1003 72 FOS-CHOLINE®-13, SOL-GRADE® F310S 61 LAPAO, SOL-GRADE® L360S 73 FOS-CHOLINE®-14, ANAGRADE® F312 62 3-Laurylamido-N,N'-dimethylpropyl aminoxide L360S 73 FOS-CHOLINE®-14, DEUTERATED F312D 62 Lauroyl sucrose S350 92 FOS-CHOLINE®-14, SOL-GRADE® F312S 62 Lauryl maltoside D310T 54 FOS-CHOLINE®-15, ANAGRADE® F314 63 Lauryl maltoside, ANAGRADE® D310 52 FOS-CHOLINE®-15, SOL-GRADE® F314S 63 Lauryl maltoside, ANAGRADE® D310A 52 FOS-CHOLINE®-16, ANAGRADE® F316 63 Lauryl maltoside, ANAGRADE® D310HA 51 FOS-CHOLINE®-16, SOL-GRADE® F316S 64 Lauryl maltoside, ANAGRADE® D310LA 53 FOS-CHOLINE®-ISO-9, ANAGRADE® FCI09 64 Lauryl maltoside, SOL-GRADE® D310S 53 FOS-CHOLINE®-ISO-11, ANAGRADE® FCI11 64 Lauryldimethylamine-N-oxide D360 49 FOS-CHOLINE®-ISO-11-6U, ANAGRADE® FCI116 65 Lauryldimethylamine-N-oxide D360S 49 FOS-CHOLINE®-UNSAT-11-10, ANAGRADE® FCU110 65 LB Broth, Ready-Made Powder L1000 73 FOS-MEA®-8, ANAGRADE® F208 65 LDAO D360 49 FOS-MEA®-10, ANAGRADE® F210 66 LDAO D360S 49 FOS-MEA®-12, ANAGRADE® F212 66 LDS L1002 73 FOSFEN™-9, ANAGRADE® FF360 66 Lithium dodecyl sulfate (LDS) L1002 73 β-D-Fructopyranosyl-α-D-glucopyranoside monododecanoate S350 92 Luria Broth, Ready-Made Powder L1001 74 Guanidine hydrochloride G1000 67 Magenta-GAL B2010 74 HECAMEG A340 20 MEGA-8, ANAGRADE® M319 74 HEGA®-8, ANAGRADE® H108 67 MEGA-9, ANAGRADE® M325 75 HEGA®-9, ANAGRADE® H109 67 MEGA-10, ANAGRADE® M320 75 HEGA®-10, ANAGRADE® H110 68 Methanesulfonothioic acid, sodium salt S110MT 91 HEGA®-11, ANAGRADE® H111 68 1-Methylethyl-1-thio-β-D-galactopyranoside I1002 72 6-O-(N-Heptylcarbamoyl)-methyl-α-D-glucopyranoside A340 20 1-Methylethyl-1-thio-β-D-galactopyranoside I1003 72 n-Heptyl-β-D-glucopyranoside, ANAGRADE® H300 68 Methyl-6-O-(N-heptylcarbamoyl)-α-D-glucopyranoside A340 20 n-Heptyl-β-D-glucoside H300 68 Methyl methanethiosulfonate M110MT 75 n-Heptyl-β-D-glucopyranoside, ANAGRADE® H300LA 69 Methyl-oxirane, polymer with oxirane P300 87 n-Heptyl- -D-glucoside H300LA 69 Methyl-oxirane, polymer with oxirane P305 87 β INDEX n-Heptyl-β-D-thioglucopyranoside, ANAGRADE® H301 69 N-Methyl-N-(1-oxododecyl)-glycine, sodium salt S300 90 n-Heptyl-β-D-thioglucoside H301 69 3-(1-Methylpiperidinium)-1-propane sulfonate ND221 76 n-Heptyl-β-D-thioglucopyranoside, ANAGRADE® H301LA 69 Micropipettes M2500 75 n-Heptyl-β-D-thioglucoside H301LA 69 MMTS M110MT 75 α-Hexadecyl-ω-hydroxy-poly(oxy-1,2-ethanediyl) APB058 21 Monosodium-N-lauryl-β-iminodipropionate D346 51
800-252-1280 • 419-891-3030 • FAX 419-891-3037 • www.anatrace.com 123 Index INDEX (continued)
Product Name/Chemical Name Cat. # Page # Product Name/Chemical Name Cat. # Page # MTSEA A110MT 20 n-Octylphosphocholine F300 57 MTSES S110MT 91 Octylphospho-n-methylethanolamine F208 65 MTSET T110MT 96 Octyl tetraethylene glycol ether T350 94 MTSL O110MT 76 Octyltetraglycol T350 94 NDSΒ-195 ND195 76 n-Octyl-β-D-thioglucopyranoside, ANAGRADE® O314 85 NDSΒ-201 ND201 76 n-Octyl-β-D-thioglucoside O314 85 NDSB-211 ND211 76 n-Octyl-β-D-thioglucopyranoside, ANAGRADE® O314LA 85 NDSΒ-221 ND221 76 n-Octyl-β-D-thioglucoside O314LA 85 NDSΒ-256 ND256 76 n-Octyl-β-D-thiomaltopyranoside, ANAGRADE® O320 86 Nonanoyl-N-hydroxyethylgluamide H109 67 n-Octyl-β-D-thiomaltoside O320 86 Nonanoyl-N-methylglucamide M325 75 (1-Oxyl-2,2,5,5-tetramethyl-D3-pyrroline-3-methyl) Nonidet P40 Substitute NIDP40 77 methanethiosulfonate O110MT 76 n-Nonyl-β-D-glucopyranoside, ANAGRADE® N324 77 n-Pentadecylphosphocholine F314 63 n-Nonyl-β-D-glucopyranoside, ANAGRADE® N324LA 77 Pentaethylene glycol monodecyl ether, ANAGRADE® P340 86 PRODUCT INFOMATION n-Nonyl-β-D-glucopyranoside, SOL-GRADE® N324S 78 Pentaethylene glycol monooctyl ether, ANAGRADE® P350 86 n-Nonyl-β-D-glucoside, ANAGRADE® N324 77 3,6,9,12,15-Pentaoxapentacosan-1-ol P340 86 n-Nonyl-β-D-glucoside, SOL-GRADE® N324S 78 3,6,9,12,15-Pentaoxatricosan-1-ol P350 86 n-Nonyl-β-D-maltopyranoside, ANAGRADE® N330 78 8-(Phenylamino)-1-naphthalenesulfonic acid 3000 n-Nonyl-β-D-maltoside N330 78 PLURONIC® F-68 P300 87 n-Nonyl-β-D-thioglucopyranoside, ANAGRADE® N335 79 PLURONIC® F-127 P305 87 n-Nonyl-β-D-thioglucoside N335 79 PMAL™-C8 P5008 87 n-Nonyl-β-D-thiomaltopyranoside, ANAGRADE® N350 79 PMAL™-C10 P5010 88 n-Nonyl-β-D-thiomaltoside N350 79 PMAL™-C12 P5012 88
NEW PRODUCTS Nonylphenylphosphocholine FF360 66 PMAL™-C16 P5016 88 n-Nonylphosphocholine F302 58 Poloxamer 188 P300 87 NOPOL-FOS™, ANAGRADE® N150 79 Polyethylene glycol (23) monododecyl ether APB035 21 Octadecyltrimethylammonium chloride O355 80 Polyethylene glycol (20) monohexadodecyl ether APB058 21 Octaethylene glycol monododecyl ether, ANAGRADE® O330 80 Poly (maleic anhydride-alt-1-decene) substituted with Octaethylene glycol monododecyl ether, Analytical Grade O330A 80 3-(Dimethylamino) propylamine P5008 87 Octanoyl-N-hydroxyethylglucamide H108 67 Poly (maleic anhydride-alt-1-dodecene) substituted with Octanoyl-N-methylglucamide M319 74 3-(Dimethylamino) propylamine P5010 88 3,6,9,12,15,18,21,24-Octaoxahexatriacontan-1-ol APO128 23 Poly (maleic anhydride-alt-1-octadecene) substituted with 3,6,9,12,15,18,21,24-Octaoxahexatriacontan-1-ol O330 80 3-(Dimethylamino) propylamine P5016 88 n-Octyl-N,N-dimethyl-3-ammonio-1-propanesulfonate AZ308 25 Poly (maleic anhydride-alt-1-tetradecene) substituted with PRODUCT LISTING Octyl dimethyl-phosphine oxide D328 48 3-(Dimethylamino) propylamine P5012 88 n-Octyl-β-D-galactopyranoside, ANAGRADE® O312 81 Poly(oxy-1,2-ethanediyl) derivs., sorbitan monododecanoate APT020 21 n-Octyl-β-D-galactoside O312 81 Poly(oxy-1,2-ethanediyl) derivs., sorbitan monododecanoate T1003 97 n-Octyl-β-D-glucopyranoside, ANAGRADE® O311 81 Poly(oxy-1,2-ethanediyl) derivs., (Z)-sorbitan n-Octyl-α-D-glucopyranoside, ANAGRADE® O311HA 82 mono-9-octadecanoate APT080 22 n-Octyl-β-D-glucopyranoside, SOL-GRADE® O311S 82 Poly(oxy-1,2-ethanediyl) derivs., (Z)-sorbitan mono-9-octadecanoate T1004 98 n-Octyl-d17-β-D-glucopyranoside O311T 83 Polyoxyethylene(6)decyl ether APO106 22 n-Octyl-d17-β-D-glucopyranoside-d7 O311D 83 n-Octyl-β-D-glucoside, ANAGRADE® O311 81 Polyoxyethylene(9)decyl ether APO109 22 n-Octyl-α-D-glucoside, ANAGRADE® O311HA 82 Polyoxyethylene(8)dodecyl ether APO128 23 n-Octyl-β-D-glucoside, SOL-GRADE® O311S 82 Polyoxyethylene(9)dodecyl ether APO129 23 Polyoxyethylene(10)dodecyl ether AP1210 23 n-Octyl-d17-β-D-glucoside-d7 O311D 83 Polyoxyethylene(8)tridecyl ether APO138 23 n-Octyl-d17-β-D-glucoside, tail deuterated O311T 83 n-Octyl-β-D-maltopyranoside, ANAGRADE® O310 84 Polyoxyethylene-polyoxypropylene block copolymer P300 87 n-Octyl-β-D-maltopyranoside, SOL-GRADE® O310S 84 Polyoxyethylene-polyoxypropylene block copolymer P305 87 n-Octyl-β-D-maltoside, ANAGRADE® O310 84 Polyoxyethylene(20)sorbitan monolaurate APT020 21 n-Octyl-β-D-maltoside, SOL-GRADE® O310S 84 Polyoxyethylene(20)sorbitan monolaurate T1003 97 Octyl pentaethylene glycol ether P350 86 Polyoxyethylene sorbitan monolaurate T1005 97 INDEX Octylpentaglycol P350 86 Polyoxyethylene(80)sorbitan monolaurate APT080 22 [Octylphenoxyl]polyethoxyethanol APND40 24 Polyoxyethylene(80)sorbitan monolaurate T1004 98 [Octylphenoxyl]polyethoxyethanol NIDP40 77 Polyoxyethylene sorbitan monooleate T1006 97
124 800-252-1280 • 419-891-3030 • FAX 419-891-3037 • www.anatrace.com Index SERVICE INFORMATION INDEX (continued)
Product Name/Chemical Name Cat. # Page # Product Name/Chemical Name Cat. # Page # 2-propyl-1-pentyl-β-D-maltopyranoside, ANAGRADE® P310 89 TRITON® X-100 APX100 24 (3-1-Pyridino)-1-propane sulfonate ND201 76 TRITON® X-100 T1001 96 SDS S1001 91 TRITON® X-114 APX114 24 L-(+)-Selenomethionine, Analytical Grade S2000 89 TRITON® X-114 T1002 97 ®
Sodium azide S3000 89 TRITON X-305 APX305 25 PRODUCT INFORMATION Sodium cholate, ANAGRADE® S1010 90 TRITON® X-405 APX405 25 Sodium cholate, SOL-GRADE® S1010S 90 TWEEN® 20 APT020 21 Sodium dodecanoyl sarcosine, ANAGRADE® S300 90 TWEEN® 20 T1003 97 Sodium dodecanoyl sarcosine, SOL-GRADE® S300S 91 TWEEN® 40 T1005 97 Sodium dodecyl sulfate, Biotech Grade S1001 91 TWEEN® 60 T1006 97 Sodium lauroyl sarcosine, ANAGRADE® S300 90 TWEEN® 80 APT080 22 Sodium lauroyl sarcosine, SOL-GRADE® S300S 91 TWEEN® 80 T1004 98 Sodium (2-sulfonatoethyl) methanethiosulfonate S110MT 91 UDAO U360 98 Sodium taurocholate, ANAGRADE® S2033 92 Undecanoyl-N-hydroxyethylglucamide H111 68 Sucrose monododecanoate, ANAGRADE® S350 92 Undecyldimethylamine-N-oxide U360 98 Sulfuric acid monododecyl ester sodium salt S1001 91 n-Undecyl-N,N-dimethylamine-oxide, ANAGRADE® U360 98
1-(3-Sulfopropyl)-pyridinium hydroxide inner salt ND201 76 NEW PRODUCTS n-Undecyl-β-D-maltopyranoside, ANAGRADE® U300 98 TCEP T1000 92 n-Undecyl-α-D-maltopyranoside, ANAGRADE® U300HA 99 n-Tetradecyl-N,N-dimethyl-3-ammonio-1-propanesulfonate AZ314 27 n-Undecyl-β-D-maltopyranoside, ANAGRADE® U300LA 99 n-Tetradecyl-N,N-dimethylamine-N-oxide, ANAGRADE® T360 93 n-Undecyl-β-D-maltopyranoside, SOL-GRADE® U300S 100 n-Tetradecyl-N,N-dimethylglycine, ANAGRADE® T305 93 n-Undecyl-β-D-maltoside, ANAGRADE® U300 98 Tetradecyl 4-O- -D-glucopyranosyl- -D-glucopyranoside T315 93 α β n-Undecyl-α-D-maltoside, ANAGRADE® U300HA 99 n-Tetradecyl- -D-maltopyranoside, ANAGRADE® T315 93 β n-Undecyl-β-D-maltoside, ANAGRADE® U300LA 99 n-Tetradecyl- -D-maltopyranoside, SOL-GRADE® T315S 94 β n-Undecyl-β-D-maltoside, SOL-GRADE® U300S 100 n-Tetradecyl- -D-maltoside, ANAGRADE® T315 93 β n-Undecyl-β-D-thiomaltopyranoside, ANAGRADE® U342 100 n-Tetradecyl- -D-maltoside, SOL-GRADE® T315S 94 β n-Undecyl-β-D-thiomaltoside, ANAGRADE® U342 100 n-Tridecylphosphocholine, ANAGRADE® F310 61 ω-Undecylenyl-β-D-maltopyranoside, ANAGRADE® U310 101 n-Tetradecylphosphocholine, ANAGRADE® F312 62 ω-Undecylenyl-β-D-maltoside, ANAGRADE® U310 101 n-Tetradecylphosphocholine, Deuterated F312D 62 10-Undecylenyl-1-phosphocholine FCU110 65 ® n-Tetradecylphosphocholine, SOL-GRADE F312S 62 n-Undecylphosphocholine, ANAGRADE® F306 59 Tetradecyltrimethylammonium chloride T355 94 n-Undecylphosphocholine, SOL-GRADE® F306S 60 ® Tetraethylene glycol monooctyl ether, ANAGRADE T350 94 Undecyl-6-phosphocholine FCI116 65 α-[(1,1,3,3-Tetramethylbutyl)phenyl]-ω-hydroxy- Urea U1000 101 poly(oxy-1,2-ethanediyl) APX114 24 X-GAL B2000 101 α-[4-(1,1,3,3-Tetramethylbutyl)phenyl]-ω-hydroxy- XGAL-IPTG B2050 102 poly(oxy-1,2-ethanediyl) APX100 24 X-Gluc, CHA salt B2020 102 α-[4-(1,1,3,3-Tetramethylbutyl)phenyl]-ω-hydroxy- X-Gluc, Na salt B2030 102 poly(oxy-1,2-ethanediyl) APX305 25 Zwittergent® 3-08 AZ308 25 α-[4-(1,1,3,3-Tetramethylbutyl)phenyl]-ω-hydroxy- Zwittergent® 3-10 AZ310 26 poly(oxy-1,2-ethanediyl) APX405 25 Zwittergent® 3-12 AZ312 26 3,6,9,12-Tetraoxaeicosan-1-ol T350 94 Zwittergent® 3-14 AZ314 27 n-Tridecyl-β-D-maltopyranoside, ANAGRADE® T323 95 n-Tridecyl-β-D-maltoside T323 95 n-Tridecyl-β-D-maltopyranoside, ANAGRADE® T323LA 95 n-Tridecyl-β-D-maltoside T323LA 95 n-Tridecyl-β-D-maltopyranoside, SOL-GRADE® T323S 96 Tridecyl-β-D-maltoside T323S 96 n-Tridecylphosphocholine, ANAGRADE® F310 61 n-Tridecylphosphocholine, SOL-GRADE® F310S 61 3α,7α,12α-Trihydroxy-5β-cholan-24-oic acid, monosodium salt S1010 90 INDEX 3α,7α,12α-Trihydroxy-5β-cholan-24-oic acid, N-(2-sulfoethyl) amide S2033 92 [2-(Trimethylammonium)ethyl] methane thiosulfonate bromide T110MT 96 Tris (carboxyethyl) phosphine hydrochloride T1000 92
800-252-1280 • 419-891-3030 • FAX 419-891-3037 • www.anatrace.com 125 Index CAS Registry Index
CAS # Cat. # Product Name Page #
57-13-6 U1000 Urea 101 57-88-5 CH200 Cholesterol 32 69-78-3 D1000 DTNB 55 82-76-8 C3000 CMC dye solution 33 69-52-3 A1000 Ampicillin, sodium salt 20 67-42-5 E1001 EGTA 57 50-01-1 G1000 Guanidine hydrochloride 67 128-53-0 E2000 NEM 57 145-42-6 S2033 Sodium taurocholate, ANAGRADE® 92 112-03-8 O355 Octadecyltrimethylammonium chloride 80 112-02-7 H355 Hexadecyltrimethylammonium chloride 70 112-00-5 D355 Dodecyltrimethylammonium chloride 55 137-16-6 S300 Sodium dodecanoyl sarcosine, ANAGRADE® 90 137-16-6 S300S Sodium dodecanoyl sarcosine, SOL-GRADE® 91 151-21-3 S1001 Sodium dodecyl sulfate, Biotech Grade 91 PRODUCT INFOMATION 302-95-4 D380 Deoxycholic acid, sodium salt, ANAGRADE® 47 288-32-4 I1001 Imidazole 71 367-93-1 I1002 Isopropyl-β-D-thiogalactopyranoside, ANAGRADE® 72 367-93-1 I1003 Isopropyl-β-D-thiogalactopyranoside, Analytical 72 367-93-1 I1003P Isopropyl-β-D-thiogalactopyranoside, Analytical 72 361-09-1 S1010 Sodium cholate, ANAGRADE® 90 361-09-1 S1010S Sodium cholate, SOL-GRADE® 90 683-10-3 D350 n-Dodecyl-N,N-dimethylglycine, ANAGRADE® 50 683-10-3 D350S n-Dodecyl-N,N-dimethylglycine, SOL-GRADE® 50 872-50-4 B2050 XGAL-IPTG 102 NEW PRODUCTS 1643-20-5 D360 n-Dodecyl-N,N-dimethylamine-N-oxide, ANAGRADE® 49 1643-20-5 D360S n-Dodecyl-N,N-dimethylamine-N-oxide,SOL-GRADE® 49 1950-85-2 S110MT Sodium (2-sulfonatoethyl) methanethiosulfonate 91 2044-56-6 L1002 LDS 73 2190-95-6 D330 Dimethyldecylphosphine oxide, ANAGRADE® 48 2497-59-8 NIDP40 Nonidet P40 Substitute 77 2605-79-0 D365 n-Decyl-N,N-dimethylamine-N-oxide, ANAGRADE® 42 2601-33-4 T305 n-Tetradecyl-N,N-dimethylglycine, ANAGRADE® 93 2644-45-3 D352 n-Decyl-N,N-dimethylglycine, ANAGRADE® 43 2949-92-0 M110MT MMTS 75 ® 3055-98-9 APO128 ANAPOE -C12E8 23 PRODUCT LISTING 3055-98-9 O330 Octaethylene glycol monododecyl ether, ANAGRADE® 80 3055-98-9 O330A Octaethylene glycol monododecyl ether, Analytical Grade 80 ® 3055-99-0 APO129 ANAPOE -C12E9 23 3211-76-5 S2000 L-(+)-Selenomethionine, Analytical Grade 89 3332-27-2 T360 n-Tetradecyl-N,N-dimethylamine-N-oxide, ANAGRADE® 93 3655-00-3 D345 n-Dodecyl-β-iminodipropionic acid, disodium salt 51 4440-54-4 H350 Hexaethylene glycol monooctyl ether, ANAGRADE® 70 4574-07-3 T355 Tetradecyltrimethylammonium chloride 94 4800-94-6 CA100 Carbenicillin disodium salt 29 ® 5168-98-9 APO106 ANAPOE -C10E6 22 6381-92-6 E1000 EDTA 56 ® 6540-99-4 AP1210 ANAPOE -C12E10 23 6892-68-8 D2001 DTE 55 7240-90-6 B2000 X-GAL, ANAGRADE® 101 9004-95-9 APB058 ANAPOE®-58 21 9001-93-1 T1001 TRITON® X-100 96 9002-93-1 APX100 ANAPOE®-X-100 24 9002-93-1 APX305 ANAPOE®-X-305 25 9002-93-1 APX405 ANAPOE®-X-405 25 9002-92-0 APB035 ANAPOE®-35 21 INDEX 9005-67-8 T1006 TWEEN® 60 97 9005-66-7 T1005 TWEEN® 40 97
126 800-252-1280 • 419-891-3030 • FAX 419-891-3037 • www.anatrace.com Index SERVICE INFORMATION
CAS Registry Index (continued) CAS # Cat. # Product Name Page #
9005-65-6 APT080 ANAPOE®-80 22 9005-65-6 T1004 TWEEN® 80 98 9005-64-5 APND40 ANAPOE®—NID-P40 24 9005-64-5 APT020 ANAPOE®-20 21 ® 9005-64-5 T1003 TWEEN 20 97 PRODUCT INFORMATION 9003-11-6 P300 PLURONIC® F-68 87 9003-11-6 P305 PLURONIC® F-127 87 ® 9043-30-5 APO138 ANAPOE -C13E8 23 9036-19-5 APX114 ANAPOE®-X-114 24 9036-19-5 T1002 TRITON® X-114 97 10108-87-9 D354 Decyltrimethylammonium chloride 47 13232-68-3 D328 Dimethyloctylphosphine oxide 48 14565-56-4 D335 n-Decyl-β-D-thiomaltopyranoside, ANAGRADE® 46 14933-09-6 AZ314 ANZERGENT® 3-14, Analytical Grade 27 14933-08-5 AZ312 ANZERGENT® 3-12, Analytical Grade 26 14960-06-6 D346 n-Dodecyl-β-iminodipropionic acid, monosodium salt 51 15178-76-4 AZ308 ANZERGENT® 3-8, Analytical Grade 25
15178-71-9 U360 n-Undecyl-N,N-dimethylamine-oxide, ANAGRADE® 98 NEW PRODUCTS 15163-36-7 AZ310 ANZERGENT® 3-10, Analytical Grade 26 15471-17-7 ND201 NDSB-201 76 16599-33-0 A110MT 2-Aminoethyl methanethiosulfonate hydrobromide 20 18449-82-6 T315 n-Tetradecyl-β-D-maltopyranoside, ANAGRADE® 93 18449-82-6 T315S n-Tetradecyl-β-D-maltopyranoside, SOL-GRADE® 94 19327-40-3 P350 Pentaethylene glycol monooctyl ether, ANAGRADE® 86 19327-39-0 T350 Tetraethylene glycol monooctyl ether, ANAGRADE® 94 23244-49-7 P340 Pentaethylene glycol monodecyl ether, ANAGRADE® 86 25339-99-5 S350 Sucrose monododecanoate, ANAGRADE® 92 ® 26183-52-8 APO109 ANAPOE -C10E9 22 26628-22-8 S3000 Sodium azide 89 27565-41-9 D2000 DTT, Cleland’s Reagent 55 27565-41-9 D2000A DTT, Cleland’s Reagent, ANAGRADE® 56 29557-51-5 F308 FOS-CHOLINE®-12, ANAGRADE® 60 29557-51-5 F308S FOS-CHOLINE®-12, SOL-GRADE® 61 29781-80-4 O311HA n-Octyl-α-D-glucopyranoside, ANAGRADE® 82 29836-26-8 O311 n-Octyl-β-D-glucopyranoside, ANAGRADE® 81 29836-26-8 O311S n-Octyl-β-D-glucopyranoside, SOL-GRADE® 82 38880-58-9 ND211 NDSB-211 76 40427-75-6 O312 n-Octyl-β-D-galactopyranoside, ANAGRADE® 81 51805-45-9 T1000 TCEP 92 53255-89-3 F300 FOS-CHOLINE®-8, ANAGRADE® 57 53255-89-3 F300S FOS-CHOLINE®-8, SOL-GRADE® 58 58066-85-6 F316 FOS-CHOLINE®-16, ANAGRADE® 63 58066-85-6 F316S FOS-CHOLINE®-16, SOL-GRADE® 64 58846-77-8 D321 n-Decyl-β-D-glucopyranoside, ANAGRADE® 43 59080-45-4 H305 n-Hexyl-β-D-glucopyranoside, ANAGRADE® 71 59122-55-3 D318 n-Dodecyl-β-D-glucopyranoside, ANAGRADE® 50 61792-31-2 L360S LAPAO, SOL-GRADE® 73 69227-93-6 D310 n-Dodecyl-β-D-maltopyranoside, ANAGRADE® 52 69227-93-6 D310A n-Dodecyl-β-D-maltopyranoside, ANAGRADE® 52 69227-93-6 D310LA n-Dodecyl-β-D-maltopyranoside, ANAGRADE® 53 69227-93-6 D310S n-Dodecyl-β-D-maltopyranoside, SOL-GRADE® 53 69984-73-2 N324 n-Nonyl-β-D-glucopyranoside, ANAGRADE® 77 69984-73-2 N324LA n-Nonyl-β-D-glucopyranoside, ANAGRADE® 77 69984-73-2 N324S n-Nonyl-β-D-glucopyranoside, SOL-GRADE® 78 INDEX 70504-28-8 F304 FOS-CHOLINE®-10, ANAGRADE® 59 70504-28-8 F304S FOS-CHOLINE®-10, SOL-GRADE® 59 75621-03-3 C316 CHAPS, ANAGRADE® 30 75621-03-3 C316S CHAPS, SOL-GRADE® 30
800-252-1280 • 419-891-3030 • FAX 419-891-3037 • www.anatrace.com 127 Index
CAS Registry Index (conintued) CAS # Cat. # Product Name Page #
77733-28-9 F312 FOS-CHOLINE®-14, ANAGRADE® 62 77733-28-9 F312S FOS-CHOLINE®-14, SOL-GRADE® 62 78617-12-6 H300 n-Heptyl-β-D-glucopyranoside, ANAGRADE® 68 78617-12-6 H300LA n-Heptyl-β-D-glucopyranoside, ANAGRADE® 69 81239-45-4 ND256 NDSB-256 76 82473-24-3 C317 CHAPSO, ANAGRADE® 31 82494-09-5 D322 n-Decyl-β-D-maltopyranoside, ANAGRADE® 44 82494-09-5 D322LA n-Decyl-β-D-maltopyranoside, ANAGRADE® 45 82494-09-5 D322S n-Decyl-β-D-maltopyranoside, SOL-GRADE® 45 82494-08-4 O310 n-Octyl-β-D-maltopyranoside, ANAGRADE® 84 82494-08-4 O310S n-Octyl-β-D-maltopyranoside, SOL-GRADE® 84 85316-98-9 M319 MEGA-8, ANAGRADE® 74 85261-20-7 M320 MEGA-10, ANAGRADE® 75 85261-19-4 M325 MEGA-9, ANAGRADE® 75 85618-21-9 O314 n-Octyl-β-D-thioglucopyranoside, ANAGRADE® 85 PRODUCT INFOMATION 85618-21-9 O314LA n-Octyl-β-D-thioglucopyranoside, ANAGRADE® 85 85618-20-8 H301 n-Heptyl-β-D-thioglucopyranoside, ANAGRADE® 69 85618-20-8 H301LA n-Heptyl-β-D-thioglucopyranoside, ANAGRADE® 69 85775-42-4 F310 FOS-CHOLINE®-13, ANAGRADE® 61 85775-42-4 F310S FOS-CHOLINE®-13, SOL-GRADE® 61 86303-23-3 B310 Big CHAP, Deoxy, Analytical Grade 28 86303-22-2 B300 Big CHAP, Analytical Grade 27 91774-25-3 T110MT [2-(Trimethylammonium)ethyl] methane thiosulfonate bromide 96 93863-88-8 B2010 Magenta-GAL 74 93911-12-7 T323 n-Tridecyl-β-D-maltopyranoside, ANAGRADE® 95 NEW PRODUCTS 93911-12-7 T323LA n-Tridecyl-β-D-maltopyranoside, ANAGRADE® 95 93911-12-7 T323S n-Tridecyl-β-D-maltopyranoside, SOL-GRADE® 96 97753-82-7 B2040 Blue-GAL 28 98064-96-1 H320 n-Hexadecyl-β-D-maltopyranoside, ANAGRADE® 70 98854-16-1 D323 n-Decyl-β-D-thioglucopyranoside, ANAGRADE® 46 98854-15-0 N335 n-Nonyl-β-D-thioglucopyranoside, ANAGRADE® 79 102601-49-0 CH210 Cholesteryl hemisuccinate tris salt 33 104702-33-2 F208 FOS-MEA®-8, ANAGRADE® 65 106402-05-5 N330 n-Nonyl-β-D-maltopyranoside, ANAGRADE® 78 114162-64-0 B2020 X-Gluc, CHA Salt 102 115457-83-5 A340 ANAMEG®-7, ANAGRADE® 20 PRODUCT LISTING 116183-64-3 D310HA n-Dodecyl-α-D-maltopyranoside, ANAGRADE® 51 121045-77-0 FCU110 FOS-CHOLINE®-UNSAT-11-10, ANAGRADE® 65 129274-39-1 F212 FOS-MEA®-12, ANAGRADE® 66 129522-81-2 O311T n-Octyl-d -β-D-glucopyranoside-d 83 17 7 129541-41-9 B2030 X-Gluc, Na Salt 102 130890-78-7 F308D FOS-CHOLINE®-12, Deuterated 60 139361-84-5 H110 HEGA®-10, ANAGRADE® 68 146801-07-2 F314 FOS-CHOLINE®-15, ANAGRADE® 63 146801-07-2 F314S FOS-CHOLINE®-15, ANAGRADE® 63 148565-58-6 D342 n-Dodecyl-β-D-thiomaltopyranoside, ANAGRADE® 54 148565-57-5 U342 n-Undecyl-β-D-thiomaltopyranoside, ANAGRADE® 100 148565-55-3 N350 n-Nonyl-β-D-thiomaltopyranoside, ANAGRADE® 79 148616-91-5 O320 n-Octyl-β-D-thiomaltopyranoside, ANAGRADE® 86 160255-06-1 ND195 NDSB-195 76 160788-56-7 ND221 NDSB-221 76 168037-13-6 U300HA n-Undecyl-α-D-maltopyranoside, ANAGRADE® 99 168037-12-5 D322HA n-Decyl-α-D-maltopyranoside, ANAGRADE® 44 181135-57-9 C324 CYMAL®-4, ANAGRADE® 38 181135-58-0 C323 CYMAL®-3, ANAGRADE® 38 228579-27-9 C326 CYMAL®-6, ANAGRADE® 40 INDEX 228579-27-9 C326LA CYMAL®-6, ANAGRADE® 40 228579-27-9 C326S CYMAL®-6, SOL-GRADE® 41
128 800-252-1280 • 419-891-3030 • FAX 419-891-3037 • www.anatrace.com Index SERVICE INFORMATION
CAS Registry Index (continued) CAS # Cat. # Product Name Page #
250692-65-0 C325 CYMAL®-5, ANAGRADE® 39 250692-65-0 C325S CYMAL®-5, SOL-GRADE® 39 253678-65-8 F306 FOS-CHOLINE®-11, ANAGRADE® 59 253678-65-8 F306S FOS-CHOLINE®-11, SOL-GRADE® 60 253678-64-7 F302 FOS-CHOLINE®-9, ANAGRADE® 58 PRODUCT INFORMATION 253678-64-7 F302S FOS-CHOLINE®-9, SOL-GRADE® 58 253678-67-0 U300 n-Undecyl-β-D-maltopyranoside, ANAGRADE® 98 253678-67-0 U300LA n-Undecyl-β-D-maltopyranoside, ANAGRADE® 99 253678-67-0 U300S n-Undecyl-β-D-maltopyranoside, SOL-GRADE® 100 260804-65-7 C322 CYMAL®-2, ANAGRADE® 37 260804-64-6 C321 CYMAL®-1, ANAGRADE® 37 349477-49-2 C327 CYMAL®-7, ANAGRADE® 41 349477-49-2 C327S CYMAL®-7, SOL-GRADE® 42 557788-85-9 F210 FOS-MEA®-10, ANAGRADE® 66 603111-75-7 C408 C-HEGA®-8, ANAGRADE® 31 657393-66-3 C518 CYCLOFOS™-7, ANAGRADE® 35 657393-65-2 C516 CYCLOFOS™-6, ANAGRADE® 35 657393-64-1 C514 CYCLOFOS™-5, ANAGRADE® 34 NEW PRODUCTS 675126-15-5 C512 CYCLOFOS™-4, ANAGRADE® 34 823796-66-3 C510 CYCLOFOS™-3, ANAGRADE® 34 823796-65-2 C508 CYCLOFOS™-2, ANAGRADE® 33
849110-74-3 D310T n-Dodecyl-d25-β-D-maltopyranoside 54 864434-16-2 C411 C-HEGA®-11, ANAGRADE® 32 864434-15-1 C410 C-HEGA®-10, ANAGRADE® 32 864434-14-0 C409 C-HEGA®-9, ANAGRADE® 31 869540-67-0 C314 3-Carboxy-ω-heptadecenamidopropyl dimethylamine 29 869547-63-7 C385 3-Carboxy-5-pentadecenamidopropyl dimethylamine, ANAGRADE® 30 869542-54-1 C324G CYGLU®-4, ANAGRADE® 36 869548-41-4 C610 CYPFOS™-3, ANAGRADE® 42 869549-38-2 C640 C-DODECAFOS™, ANAGRADE® 28 869550-29-8 C630 C-OCTAFOS™, ANAGRADE® 29 869541-00-4 C323G CYGLU®-3, ANAGRADE® 36 869638-98-2 F312D FOS-CHOLINE®-14, DEUTERATED 62 869648-98-6 FF360 FOSFEN™-9, ANAGRADE® 66 869646-90-2 FCI09 FOS-CHOLINE®-ISO-9, ANAGRADE® 64 869653-90-7 H109 HEGA®-9, ANAGRADE® 67 869647-81-4 FCI116 FOS-CHOLINE®-ISO-11-6U, ANAGRADE® 65 869660-84-4 N150 NOPOL-FOS™, ANAGRADE® 79 869647-65-4 FCI11 FOS-CHOLINE®-ISO-11, ANAGRADE® 64 869584-01-0 D412 DIFOS™-12, ANAGRADE® 47 869652-63-1 H108 HEGA®-8, ANAGRADE® 67 869666-57-9 O311D n-Octyl-d -β-D-glucopyranoside-d 83 17 7 869638-31-3 DH325 2,6-Dimethyl-4-heptyl-β-D-maltopyranoside, ANAGRADE® 48 869704-76-7 U310 ω-Undecylenyl-β-D-maltopyranoside, ANAGRADE® 101 869654-10-4 H111 HEGA®-11, ANAGRADE® 68 869668-28-0 P310 2-Propyl-1-pentyl maltopyranoside, ANAGRADE® 89 869796-48-5 C702 CYGENT™ 1-3, ANAGRADE® 35 869856-84-8 P5008 PMAL™-C8 87 869856-88-2 P5010 PMAL™-C10 88 869857-16-9 P5016 PMAL™-C16 88 869857-14-7 P5012 PMAL™-C12 88 870287-95-9 H310 n-Hexyl-β-D-maltopyranoside, ANAGRADE® 71 INDEX
800-252-1280 • 419-891-3030 • FAX 419-891-3037 • www.anatrace.com 129