Microbial Assemblages Reflect Environmental Heterogeneity in Alpine Streams
Received: 8 November 2018 | Revised: 1 April 2019 | Accepted: 26 April 2019 DOI: 10.1111/gcb.14683 PRIMARY RESEARCH ARTICLE Microbial assemblages reflect environmental heterogeneity in alpine streams Scott Hotaling1 | Mary E. Foley1,2 | Lydia H. Zeglin3 | Debra S. Finn4 | Lusha M. Tronstad5 | J. Joseph Giersch6 | Clint C. Muhlfeld6,7 | David W. Weisrock1 1Department of Biology, University of Kentucky, Lexington, Kentucky Abstract 2Biology Department, Rutgers, The State Alpine streams are dynamic habitats harboring substantial biodiversity across small University of New Jersey, Camden, New spatial extents. The diversity of alpine stream biota is largely reflective of environ‐ Jersey 3Division of Biology, Kansas State mental heterogeneity stemming from varying hydrological sources. Globally, alpine University, Manhattan, Kansas stream diversity is under threat as meltwater sources recede and stream conditions 4 Department of Biology, Missouri State become increasingly homogeneous. Much attention has been devoted to macroin‐ University, Springfield, Missouri vertebrate diversity in alpine headwaters, yet to fully understand the breadth of cli‐ 5Wyoming Natural Diversity Database, University of Wyoming, Laramie, mate change threats, a more thorough accounting of microbial diversity is needed. Wyoming We characterized microbial diversity (specifically Bacteria and Archaea) of 13 6U.S. Geological Survey, Northern Rocky Mountain Science Center, West Glacier, streams in two disjunct Rocky Mountain subranges through 16S rRNA gene sequenc‐ Montana ing. Our study encompassed the spectrum of alpine stream sources (glaciers, snow‐ 7 Flathead Lake Biological Station, The fields, subterranean ice, and groundwater) and three microhabitats (ice, biofilms, and University of Montana, Polson, Montana streamwater). We observed no difference in regional (γ) diversity between subranges Correspondence but substantial differences in diversity among ( ) stream types and microhabitats.
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