ACER1 Antibody Cat. No.: 60-034

ACER1 Antibody

Specifications

HOST SPECIES: Rabbit

SPECIES REACTIVITY: Human

This ACER1 antibody is generated from rabbits immunized with a KLH conjugated IMMUNOGEN: synthetic peptide between 236-263 amino acids from the C-terminal region of human ACER1.

TESTED APPLICATIONS: WB

APPLICATIONS: For WB starting dilution is: 1:1000

PREDICTED MOLECULAR 31 kDa WEIGHT:

Properties

This antibody is purified through a protein A column, followed by peptide affinity PURIFICATION: purification.

CLONALITY: Polyclonal

ISOTYPE: Rabbit Ig

CONJUGATE: Unconjugated

September 25, 2021 1 https://www.prosci-inc.com/acer1-antibody-60-034.html PHYSICAL STATE: Liquid

BUFFER: Supplied in PBS with 0.09% (W/V) sodium azide.

CONCENTRATION: batch dependent

Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies STORAGE CONDITIONS: care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Additional Info

OFFICIAL SYMBOL: ACER1

Alkaline 1, AlkCDase 1, Alkaline CDase 1, Acylsphingosine deacylase 3, N- ALTERNATE NAMES: acylsphingosine amidohydrolase 3, ACER1, ASAH3

ACCESSION NO.: Q8TDN7

PROTEIN GI NO.: 74715919

GENE ID: 125981

USER NOTE: Optimal dilutions for each application to be determined by the researcher.

Background and References

Ceramides are synthesized during epidermal differentiation and accumulate within the interstices of the stratum corneum, where they represent critical components of the epidermal permeability barrier. Excess cellular can trigger antimitogenic signals and induce apoptosis, and the ceramide metabolites and sphingosine-1- phosphate (S1P) are important bioregulatory molecules. Ceramide hydrolysis in the BACKGROUND: nucleated cell layers regulates keratinocyte proliferation and apoptosis in response to external stress. Ceramide hydrolysis also occurs at the stratum corneum, releasing free sphingoid base that functions as an endogenous antimicrobial agent. ACER1 is highly expressed in epidermis and catalyzes the hydrolysis of very long chain to generate sphingosine (Houben et al., 2006 [PubMed 16477081]; Sun et al., 2008 [PubMed 17713573]).

REFERENCES: 1) Sun, W., et al. J. Invest. Dermatol. 128(2):389-397(2008)

2) Toulza, E., et al. Genome Biol.(6), R107 (2007) :

3) Houben, E., et al. J. Lipid Res. 47(5):1063-1070(2006)

4) Mao, C., et al. J. Biol. Chem. 278(33):31184-31191(2003)

ANTIBODIES FOR RESEARCH USE ONLY.

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