Anti-mLST8/GBL (C-terminal) produced in rabbit, affinity isolated antibody

Catalog Number SAB4200306

Product Description Precautions and Disclaimer Anti-mLST8/GBL (C-terminal) is produced in rabbit This product is for R&D use only, not for drug, using as immunogen a synthetic peptide corresponding household, or other uses. Please consult the Material to a sequence located near the C-terminus of human Safety Data Sheet for information regarding hazards mLST8 (GeneID: 64223), conjugated to KLH. The and safe handling practices. corresponding sequence is identical in mouse, rat, canine, bovine and monkey. The antibody is affinity- Storage/Stability purified using the immunizing peptide immobilized on For continuous use, store at 2-8°C for up to one month. agarose. For extended storage freeze in working aliquots. Repeated freezing and thawing is not recommended. If Anti-mLST8/GBL (C-terminal) recognizes human slight turbidity occurs upon prolonged storage, clarify mLST8. The antibody may be used in various the solution by centrifugation before use. Working immunochemical techniques including immunoblotting dilution samples should be discarded if not used within (~38 kDa) and immunoprecipitation. Detection of the 12 hours. mLST8 band by immunoblotting is specifically inhibited by the immunizing peptide. Product Profile Immunoblotting: a working concentration of 1-2 mg/mL mLST8, also known as GbL (G beta-subunit-like is recommended using whole extracts of HEK-293T protein) is a widely expressed protein which consists cells over expressing human mLST8. almost entirely of seven WD40 repeats with high sequence similarity to those in b subunits of Immunoprecipitation: a working amount of 10-20 mg is heterotrimeric G . mLST8 is conserved among recommended using lysates of human A431 cells. all eukaryotes. mLST8 is a subunit of the mTOR- signaling complexes, mTORC1 and mTORC2. In Note: In order to obtain best results in different addition to mLST8, mTORC1 complex contains mTOR, techniques and preparations we recommend and raptor, a target of rapamycin, while mTORC2 determining optimal working concentration by titration complex, which is insensitive to rapamycin, includes test. mTOR and rictor. mLST8 associates with the kinase domain of mTOR and stimulates mTOR kinase activity. References mLST8 plays a critical role in nutrient-sensitive 1. Kim, D.H, et al., Mol. Cell, 11, 895-904 (2003). interaction of raptor with mTOR and in raptor-mediated 2. Sarbassov, D.D., et al., Curr. Biol., 14, 1296-1302 inhibition of mTOR kinase activity. A decrease in (2004). mLST8 expression has been shown to reduce in vivo 3. Guertin, D.A., et al., Develop. Cell, 11, 859-871 phosphorylation of S6K1.1-4 (2006). 4. Wang, L., et al., J. Biol. Chem., 281, 24293-24303 Reagent (2006). Supplied as a solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide as a ST,RC,KAA,PHC 07/11-1 preservative.

Antibody Concentration: ~ 1.0 mg/mL

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