Estrella Urtubey 12/5/2011 1

Subfamily Barnadesioideae: “New questions”

E. Urtubey 1 & T. F. Stuessy 2

1 Instituto de Botánica Darwinion, San Isidro, Argentina. 2 Department of Systematic and Evolutionary Botany, Faculty Center of Botany, University of Vienna, Austria

INSTITUTO DE BOTÁNICA DARWINION

ACADEMIA NACIONAL DE CIENCIAS EXACTAS, FÍSICAS Y NATURALES CONSEJO NACIONAL DE INVESTIGACIONES CIENTÍFICAS Y TÉCNICAS 2

Bremer (1987)

3 Molecular data

-Absence of chloroplast inversions

Morphological data

4 5 Phylogenetic hypotheses in Barnadesioideae

Stuessy & al. (1996)

Bremer (1994)

Gustafsson et al. (2001)

Urtubey & Stuessy (2001)

Gruenstaeudl et al. (2009)

6 Relationships that are clear for eight of the nine genera

• Duseniella, Doniophyton are closely related to

• is closely related to

• is closely related to and these close to the Barnadesia- Huarpea clade

• is biphyletic

But

the position of Schlechtendalia is still uncertain

7 Stuessy et al. 2009

8 9 Schlechtendalia luzulifolia

X=8

10 Pseudobilabiate 4+1

11 Distribution: Pampean phytogeographical province

GOOGLE EARTH

12 13 Montevideo, Uruguay Kiyú, Uruguay

14 Piriápolis, Uruguay

15 Illescas, Uruguay

16 RN7, Uruguay

17 PN El Palmar, Argentina 18 In Brazil,

Schlechtendalia luzulifolia

is considered in

danger of extinction

(SEMA 2003)

19 Objetive

Since Schlechtendalia is an ancient genus, in danger of extinction and lives on very disturbed areas

Population conservation: with an appropiate and efficient protection strategy applying genetic diversity

20 Question about Schlechtendalia

• What is the genetic diversity among and within populations?

21 First step:Categorizing areas

1. Protected areas

2. Pristine areas

3. Modified areas

22 Second step: Using ISSR

1. Variability among and within populations (AMOVA)

2. Similarity value (Index)

3. Relationships of populations (UPGMA)

23 Postulations

• Interrelationships among populations

• Relationships between genetic diversity and area (natural and modified)

• Natural and human isolate barriers

Areas to be preserved

24 Protected areas

PN El Palmar

Illescas Pristine areas

Punta Ballena Modified areas

www.mvotma.gub.uy/mapa-dinamico-de-areas-protegidas.

25 At present, we are working on six populations (one of Argentina and the rest of Uruguay) and comparing these populations we found ………………………

26 We continue working

Thanks! 27 Acknowledgements

Consejo Nacional de Investigaciones Científica y Técnicas (CONICET), Argentina

Organizing Committee of the South American Compositae Meeting

To E. Urtubey

28