APPLICATION NOTE

LSC COCKTAILS ). 3 O 2 ficiency O 2 ef H 3 optimal e shown below: e ed the 43.7% is less quenching 48.2% ) 2 Cl number 6013579), then 2 esults ar t ee chlorine atoms, i.e., CHCl efficiency of ULTIMA Gold efficiency of ULTIMA (thr H 3 m ofor than chlor dichloromethane/methanol/water. This change dichloromethane/methanol/water. (two may be of benefit since dichloromethane chlorine atoms, i.e., CH We tested the system using the specified chloroform/ tested the system using We Our tests were performedmethanol/water mixture. Gold in polyethylene vials by adding with ULTIMA Gold. to 10.0 mL ULTIMA 1.0 mL of the sample to help determine also varied the procedure We which conditions might lead to a mor counting situation. The variations included: 1. Increasing the volume of cocktail. 2. Changing from chloroform/methanol/water to of the total system. The r (10 mL and 15 mL) and ULTIMA-Flo M (10 mL and (10 mL and 15 mL) and ULTIMA-Flo 15 mL) (PerkinElmer par since these areadded 1 mL of the different mixtures, the main quenchers, and measur We measured the measured We ™ H 3 58.5% 47.5% 20.2% 58.5% No Sample +Dichloromethane/MeOH/H cocktail easonable ™ A-525 flow ™ ove the efficiency was achieving r y cocktails without better results. ™ cher wanted help to impr eff. 25% to 30%) by running the experiment -Flo M (15 mL) m, 9.0 mL methanol and 1.0 mL water.

H 3 TIMA Gold (15 mL) TIMA esear ofor esent, the laborator TIMA Gold (10 mL) TIMA-Flo M (10 mL) 47.5% 31.5%

ULTIMA Gold (10 mL)ULTIMA UL M (10 mL)ULTIMA-Flo UL 58.5% 47.5% 38.4% 13.5% Cocktail No Sample +Chloroform/MeOH/H using several of PerkinElmer’s safer ULTIMA-Flo using several of PerkinElmer’s pr UL Gold (15 mL)ULTIMA UL M (15 mL)ULTIMA-Flo 58.5% 47.5% 52.3% 36.0% Cocktail results ( The r (PerkinElmer part number 6013329). of his results. in a conventional LSC counter in 20 mL polyethylene ULTIMA vials with PerkinElmer’s Gold efficiency of a PerkinElmer FLO-ONE efficiency of a PerkinElmer www.perkinelmer.com The researcher’s sample was composed of 20.0 mL The researcher’s chlor Discussion and classical Flo-Scint Problem asked for application assis-Our field representative tance wished to improve the for a researcher who Quenching Effects of Chloroform of Effects Quenching LSC in Practice LSC At The researcher had attempted to improve counting results The researcher had attempted to improve by scintillation analyzer with a sample that contained scintillation analyzer the researcher hadchloroform. In general, been results. experiencing poor These results indicate the following: Recommendations Increasing the volume of cocktail from 10 mL to 15 mL After this review, we recommended changing from increases the efficiency by: the highly quenching chloroform to less quenching dichloromethane. In addition, if the researcher can • Approximately 5% for ULTIMA Gold with added adjust the ratio of cocktail to eluent such that there is chloroform/MeOH/H O [i.e., 38.4% up to 43.7%]. 2 additional cocktail, the counting efficiency would be • Approximately 7% for ULTIMA-Flo M with added increased. The biggest benefit will be seen by changing chloroform/MeOH/H O [i.e., 13.5% up to 20.2%]. 2 from chloroform to dichloromethane. In addition, when dichloromethane is used instead of chloroform the efficiency increase is: Follow-Up Comment • 10% for ULTIMA Gold (10 mL) We received a fax from this researcher which confirmed that [i.e., 38.4% up to 48.2%]. the chloroform has been replaced with dichloromethane • 8.5% for ULTIMA Gold (15 mL) and the chromatogram was not altered. Furthermore, a [i.e., 43.7% up to 52.3%]. larger volume FSA cell (5 mL) and mixing ratio of 1:5 (Sample:ULTIMA-Flo M) was being used. The efficiency • 18% for ULTIMA-Flo M (10 mL) had increased to 18% to 19% allowing the laboratory to [i.e., 13.5% up to 31.5%]. run the experiment using the FLO-ONE without fraction • 16% for ULTIMA-Flo M (15 mL) collection, as they had hoped. [i.e., 20.2% up to 36.0%].

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