JUNE 2013 • VOLUME 87 • NO. 12

TABLE OF CONTENTS

EDITORIAL In Memoriam Kuan-Teh Jeang (1958-2013): Retrovirologist Par Karen Beemon, Ben Berkhout 6539–6540 Excellence and Founding Editor of Retrovirology SPOTLIGHT Articles of Significant Interest Selected from This Issue by the 6541 Editors STRUCTURE AND ASSEMBLY Structure of Severe Fever with Thrombocytopenia Syndrome Lianying Jiao, Songying Ouyang, 6829–6839 Nucleocapsid in Complex with Suramin Reveals Mifang Liang, Fengfeng Niu, Neil Shaw, Therapeutic Potential Wei Wu, Wei Ding, Cong Jin, Yao Peng, Yanping Zhu, Fushun Zhang, Tao Wang, Chuan Li, Xiaobing Zuo, Chi-Hao Luan, Dexin Li, Zhi-Jie Liu Identification of the Herpesvirus 6A gQ1 Domain Takahiro Maeki, Mayuko Hayashi, 7054–7063 Essential for Its Functional Conformation Akiko Kawabata, Huamin Tang, Koichi Yamanishi, Yasuko Mori The Structure of the Herpes Simplex Virus DNA-Packaging Sundaresan Selvarajan Sigamani, 7140–7148 Terminase pUL15 Nuclease Domain Suggests an Evolutionary Haiyan Zhao, Yvonne N. Kamau, Joel Lineage among Eukaryotic and Prokaryotic D. Baines, Liang Tang Structure of a Classical Broadly Neutralizing Stem Antibody in Cyrille Dreyfus, Damian C. Ekiert, Ian 7149–7154 Complex with a Pandemic H2 Influenza Virus Hemagglutinin A. Wilson Evidence in Support of RNA-Mediated Inhibition of Vineela Chukkapalli, Jingga Inlora, 7155–7159 Phosphatidylserine-Dependent HIV-1 Gag Membrane Binding Gabrielle C. Todd, Akira Ono in Cells Structure of the Tetramerization Domain of Measles Virus Guillaume Communie, Thibaut Crépin, 7166–7169 Phosphoprotein Damien Maurin, Malene Ringkjøbing Jensen, Martin Blackledge, Rob W. H. Ruigrok HIV-1 Envelope Glycoprotein Trimers Display Open Audray K. Harris, Alberto Bartesaghi, 7191–7196 Quaternary Conformation When Bound to the gp41 Membrane- Jacqueline L. S. Milne, Sriram Proximal External-Region-Directed Broadly Neutralizing Subramaniam Antibody Z13e1 GENOME REPLICATION AND REGULATION OF VIRAL GENE EXPRESSION Mutational Inactivation of Herpes Simplex Virus 1 MicroRNAs Omar Flores, Sanae Nakayama, Adam 6589–6603 Identifies Viral mRNA Targets and Reveals Phenotypic Effects in W. Whisnant, Hassan Javanbakht, Culture Bryan R. Cullen, David C. Bloom EWSR1 Binds the Hepatitis C Virus cis-Acting Replication Todd E. Oakland, Kyle J. Haselton, 6625–6634 Element and Is Required for Efficient Viral Replication Glenn Randall Different Distributions of Epstein-Barr Virus Early and Late Atsuko Sugimoto, Yoshitaka Sato, Teru 6693–6699 Gene Transcripts within Viral Replication Compartments Kanda, Takayuki Murata, Yohei Narita, Daisuke Kawashima, Hiroshi Kimura, Tatsuya Tsurumi A Novel Victorivirus from a Phytopathogenic Fungus, Rosellinia Sotaro Chiba, Yu-Hsin Lin, Hideki 6727–6738 necatrix, Is Infectious as Particles and Targeted by RNA Kondo, Satoko Kanematsu, Nobuhiro Silencing Suzuki

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The Adenovirus L4-33K Protein Regulates both Late Gene Kai Wu, Diana Guimet, Patrick 6739–6747 Expression Patterns and Viral DNA Packaging Hearing Kaposi’s Sarcoma-Associated Herpesvirus (KSHV) Latency- Kevin Y. Kim, Steve B. Huerta, Chie 6782–6793 Associated Nuclear Antigen Regulates the KSHV Epigenome by Izumiya, Don-Hong Wang, Anthony Association with the Histone Demethylase KDM3A Martinez, Bogdan Shevchenko, Hsing- Jien Kung, Mel Campbell, Yoshihiro Izumiya Novel cis-Acting Element within the -Coding Region Zhong-Yu Liu, Xiao-Feng Li, Tao Jiang, 6804–6818 Enhances Flavivirus Viral-RNA Replication by Regulating Yong-Qiang Deng, Hui Zhao, Hong- Genome Cyclization Jiang Wang, Qing Ye, Shun-Ya Zhu, Yang Qiu, Xi Zhou, E-De Qin, Cheng- Feng Qin The N Terminus of Andes Virus L Protein Suppresses mRNA Patrick Heinemann, Jonas Schmidt- 6975–6985 and Protein Expression in Mammalian Cells Chanasit, Stephan Günther GENETIC DIVERSITY AND EVOLUTION Complete Genome Sequences of Elephant Endotheliotropic Gavin S. Wilkie, Andrew J. Davison, 6700–6712 Herpesviruses 1A and 1B Determined Directly from Fatal Cases Mick Watson, Karen Kerr, Stephanie Sanderson, Tim Bouts, Falko Steinbach, Akbar Dastjerdi A Case for the Ancient Origin of Coronaviruses Joel O. Wertheim, Daniel K. W. Chu, 7039–7045 Joseph S. M. Peiris, Sergei L. Kosakovsky Pond, Leo L. M. Poon Identification of a Pegivirus (GB Virus-Like Virus) That Infects Amit Kapoor, Peter Simmonds, John 7185–7190 Horses M. Cullen, Troels K. H. Scheel, Jan L. Medina, Federico Giannitti, Eiko Nishiuchi, Kenny V. Brock, Peter D. Burbelo, Charles M. Rice, W. Ian Lipkin VIRUS- INTERACTIONS Use of G-Protein-Coupled and -Uncoupled CCR5 Receptors by Reem Berro, Anila Yasmeen, Ravinder 6569–6581 CCR5 Inhibitor-Resistant and -Sensitive Human Abrol, Bartosz Trzaskowski, Sarya Abi- Immunodeficiency Virus Type 1 Variants Habib, Amy Grunbeck, Danny Lascano, William A. Goddard III, Per Johan Klasse, Thomas P. Sakmar, John P. Moore Downregulation of N mRNA Occurs through Kimihiro Hino, Hiroki Sato, Akihiro 6582–6588 Interaction between Its 3= Untranslated Region and hnRNP D Sugai, Masahiko Kato, Misako Yoneda, Chieko Kai Tropism of and Innate Immune Responses to the Novel Human Renee W. Y. Chan, Michael C. W. 6604–6614 Betacoronavirus Lineage C Virus in Human Ex Vivo Respiratory Chan, Sudhakar Agnihothram, Louisa Organ Cultures L. Y. Chan, Denise I. T. Kuok, Joanne H. M. Fong, Y. Guan, Leo L. M. Poon, Ralph S. Baric, John M. Nicholls, J. S. Malik Peiris Vesicular Stomatitis Virus Variants Selectively Infect and Kill Guido Wollmann, John N. Davis, 6644–6659 Human Melanomas but Not Normal Melanocytes Marcus W. Bosenberg, Anthony N. van den Pol

A Variant -Tropic Human Immunodeficiency Virus Rajesh Thippeshappa, Hongmei Ruan, 6678–6692 Type 1 Is Resistant to Alpha Interferon-Induced Restriction in Weiming Wang, Paul Zhou, Jason T. Pig-Tailed Macaque CD4؉ T Cells Kimata Human La Protein Interaction with GCAC near the Initiator Anuj Kumar, Upasana Ray, Saumitra 6713–6726 AUG Enhances Hepatitis C Virus RNA Replication by Das Promoting Linkage between 5= and 3= Untranslated Regions

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Ets-1 Is Required for the Activation of VEGFR3 during Latent Kimberley D. Gutierrez, Valerie A. 6758–6768 Kaposi’s Sarcoma-Associated Herpesvirus Infection of Morris, David Wu, Serge Barcy, Endothelial Cells Michael Lagunoff Foot-and-Mouth Disease Virus Modulates Cellular Vimentin for D. P. Gladue, V. O’Donnell, R. Baker- 6794–6803 Virus Survival Branstetter, L. G. Holinka, J. M. Pacheco, I. Ferna´ndez Sainz, Z. Lu, X. Ambroggio, L. Rodriguez, M. V. Borca New Model for the Genesis and Maturation of Viroplasms Qianzhuo Mao, Shenglan Zheng, 6819–6828 Induced by Fijiviruses in Insect Vector Cells Qingmei Han, Hongyan Chen, Yuanyuan Ma, Dongsheng Jia, Qian Chen, Taiyun Wei Rotavirus-Encoded Nonstructural Protein 1 Modulates Cellular Rahul Bhowmick, Umesh Chandra 6840–6850 Apoptotic Machinery by Targeting Tumor Suppressor Protein Halder, Shiladitya Chattopadhyay, p53 Mukti Kant Nayak, Mamta Chawla-Sarkar Syndecan-1 Serves as the Major Receptor for Attachment of Qing Shi, Jieyun Jiang, Guangxiang Luo 6866–6875 Hepatitis C Virus to the Surfaces of Hepatocytes

A Vicilin-Like Seed Storage Protein, PAP85, Is Involved in Cheng-En Chen, Kuo-Chen Yeh, Shu- 6888–6900 Tobacco Mosaic Virus Replication Hsing Wu, Hsiang-Iu Wang, Hsin- Hung Yeh Differing Effects of Herpes Simplex Virus 1 and Pseudorabies David Pasdeloup, Marc Labetoulle, 7102–7112 Virus Infections on Centrosomal Function Frazer J. Rixon Basic Residues in the Matrix Domain and Multimerization Fei Li, Jing Jin, Christin Herrmann, 7113–7126 Target Gag to the Virological Synapse Walther Mothes Canine Distemper Virus Associated with a Lethal Outbreak in Kouji Sakai, Tomoki Yoshikawa, Fumio 7170–7175 Monkeys Can Readily Adapt To Use Human Receptors Seki, Shuetsu Fukushi, Maino Tahara, Noriyo Nagata, Yasushi Ami, Tetsuya Mizutani, Ichiro Kurane, Ryoji Yamaguchi, Hideki Hasegawa, Masayuki Saijo, Katsuhiro Komase, Shigeru Morikawa, Makoto Takeda

Sodium Taurocholate Cotransporting Polypeptide Mediates Guocai Zhong, Huan Yan, Haimin 7176–7184 Woolly Hepatitis Infection of Tupaia Wang, Wenhui He, Zhiyi Jing, Yonghe Hepatocytes Qi, Liran Fu, Zhenchao Gao, Yi Huang, Guangwei Xu, Xiaofeng Feng, Jianhua Sui, Wenhui Li CELLULAR RESPONSE TO INFECTION Genome-Wide Analysis of Host mRNA Translation during Hélène Colman, Catherine Le Berre- 6668–6677 Hepatitis C Virus Infection Scoul, Céline Hernandez, Sandra Pierredon, Audrey Bihouée, Rémi Houlgatte, Stephan Vagner, Arielle R. Rosenberg, Cyrille Féray CD27-CD70 Costimulation Controls T Cell Immunity during Suzanne P. M. Welten, Anke Redeker, 6851–6865 Acute and Persistent Cytomegalovirus Infection Kees L. Franken, Chris A. Benedict, Hideo Yagita, Felix M. Wensveen, Jannie Borst, Cornelis J. M. Melief, René A. W. van Lier, Klaas P. J. M. van Gisbergen, Ramon Arens Impact of H216 on the DNA Binding and Catalytic Activities of Stefan Harjes, William C. Solomon, 7008–7014 the HIV Restriction Factor APOBEC3G Ming Li, Kuan-Ming Chen, Elena Harjes, Reuben S. Harris, Hiroshi Matsuo

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Divergent Kinetics of Proliferating T Cell Subsets in Simian Xiaolei Wang, Huanbin Xu, Bapi 7032–7038 Immunodeficiency Virus (SIV) Infection: SIV Eliminates the Pahar, Andrew A. Lackner, Ronald S. First Responder” CD4؉ T Cells in Primary Infection Veazey“ CDK9-Dependent Transcriptional Elongation in the Innate Bing Tian, Yingxin Zhao, Mridul 7075–7092 Interferon-Stimulated Gene Response to Respiratory Syncytial Kalita, Chukwudi B. Edeh, Slobodan Virus Infection in Airway Epithelial Cells Paessler, Antonella Casola, Michael N. Teng, Roberto P. Garofalo, Allan R. Brasier GENE DELIVERY Novel Recombinant Vectors Efficiently Deliver Ran Hong, Weiya Bai, Jianwei Zhai, 6615–6624 Protein and RNA Encoding Genes into Primary Hepatocytes Wei Liu, Xinyan Li, Jiming Zhang, Xiaoxian Cui, Xue Zhao, Xiaoli Ye, Qiang Deng, Pierre Tiollais, Yumei Wen, Jing Liu, Youhua Xie Potential of Herpesvirus Saimiri-Based Vectors To Reprogram a Hannah F. Brown, Christian Unger, 7127–7139 Somatic Ewing’s Sarcoma Family Tumor Cell Line Adrian Whitehouse VACCINES AND ANTIVIRAL AGENTS Chimeric Hemagglutinin Influenza Virus Vaccine Constructs Florian Krammer, Natalie Pica, Rong 6542–6550 Elicit Broadly Protective Stalk-Specific Antibodies Hai, Irina Margine, Peter Palese Complete Protection against Severe Acute Respiratory Craig Fett, Marta L. DeDiego, Jose A. 6551–6559 Syndrome Coronavirus-Mediated Lethal Respiratory Disease in Regla-Nava, Luis Enjuanes, Stanley Aged Mice by Immunization with a Mouse-Adapted Virus Perlman Lacking E Protein Virus Host Range E2 Transmembrane Deletion Amanda Piper, Mariana Ribeiro, 6748–6757 Mutants Induce Protective Immunity against Challenge in Katherine M. Smith, Caitlin M. Briggs, C57BL/6J Mice Emerson Huitt, Kavita Nanda, Carla J. Spears, Michelle Quiles, John Cullen, Malcolm E. Thomas, Dennis T. Brown, Raquel Hernandez A Herpes Simplex Virus Scaffold Peptide That Binds the Portal Kui Yang, Elizabeth Wills, Joel D. 6876–6887 Vertex Inhibits Early Steps in Viral Replication Baines Replication and Immunogenicity of Swine, Equine, and Avian Mariana Baz, Myeisha Paskel, Yumiko 6901–6910 H3 Subtype Influenza Viruses in Mice and Ferrets Matsuoka, James Zengel, Xing Cheng, Hong Jin, Kanta Subbarao Sulfamoylbenzamide Derivatives Inhibit the Assembly of Matthew R. Campagna, Fei Liu, 6931–6942 Hepatitis B Virus Nucleocapsids Richeng Mao, Courtney Mills, Dawei Cai, Fang Guo, Xuesen Zhao, Hong Ye, Andrea Cuconati, Haitao Guo, Jinhong Chang, Xiaodong Xu, Timothy M. Block, Ju-Tao Guo Mucosal Immunization of Lactating Female Rhesus Monkeys Genevieve G. A. Fouda, Joshua D. 6986–6999 with a Transmitted/Founder HIV-1 Envelope Induces Strong Amos, Andrew B. Wilks, Justin Pollara, -Specific IgA Antibody Responses in Breast Milk Caroline A. Ray, Anjali Chand, Erika L. Kunz, Brooke E. Liebl, Kaylan Whitaker, Angela Carville, Shannon Smith, Lisa Colvin, David J. Pickup, Herman F. Staats, Glenn Overman, Krissey Eutsey-Lloyd, Robert Parks, Haiyan Chen, Celia LaBranche, Susan Barnett, Georgia D. Tomaras, Guido Ferrari, David C. Montefiori, Hua-Xin Liao, Norman L. Letvin, Barton F. Haynes, Sallie R. Permar

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Inhibition of Hepatitis C Virus (HCV) Replication by Specific Chang Ho Lee, Young Ju Lee, Ji Hyun 7064–7074 RNA Aptamers against HCV NS5B RNA Replicase Kim, Jong Hoon Lim, Jung-Hye Kim, Wonkyo Han, Soo-Han Lee, Gyu-Jeong Noh, Seong-Wook Lee PATHOGENESIS AND IMMUNITY Vaxfectin Adjuvant Improves Antibody Responses of Juvenile Wen-Hsuan W. Lin, Adrian Vilalta, 6560–6568 Rhesus to a DNA Vaccine Encoding the Measles Virus Robert J. Adams, Alain Rolland, Sean Hemagglutinin and Fusion M. Sullivan, Diane E. Griffin Identification of Virulence Determinants within the L Genomic Lisa McLay, Shuiyun Lan, Aftab Ansari, 6635–6643 Segment of the Pichinde Yuying Liang, Hinh Ly Toscana Virus NSs Protein Inhibits the Induction of Type I Gianni Gori-Savellini, Melissa 6660–6667 Interferon by Interacting with RIG-I Valentini, Maria Grazia Cusi Sustained Hyperresponsiveness of Dendritic Cells Is Associated Sandy Pelletier, Nathalie Bédard, Elias 6769–6781 with Spontaneous Resolution of Acute Hepatitis C Said, Petronela Ancuta, Julie Bruneau, Naglaa H. Shoukry Interleukin-22 Reduces Lung Inflammation during Influenza A Stoyan Ivanov, Joelle Renneson, Josette 6911–6924 Virus Infection and Protects against Secondary Bacterial Fontaine, Adeline Barthelemy, Infection Christophe Paget, Elodie Macho Fernandez, Fany Blanc, Carl De Trez, Laurye Van Maele, Laure Dumoutier, Michel-René Huerre, Gérard Eberl, Mustapha Si-Tahar, Pierre Gosset, Jean Christophe Renauld, Jean Claude Sirard, Christelle Faveeuw, François Trottein Visualizing the Beta Interferon Response in Mice during Carsten Kallfass, Stefan Lienenklaus, 6925–6930 Infection with Influenza A Viruses Expressing or Lacking Siegfried Weiss, Peter Staeheli Nonstructural Protein 1 Comprehensive Analysis of Varicella-Zoster Virus Proteins Tihana Lenac Roviš, Susanne M. Bailer, 6943–6954 Using a New Monoclonal Antibody Collection Venkata R. Pothineni, Werner J. D. Ouwendijk, Hrvoje Šimic´, Marina Babic´, Karmela Miklic´, Suzana Malic´, Marieke C. Verweij, Armin Baiker, Orland Gonzalez, Albrecht von Brunn, Ralf Zimmer, Klaus Früh, Georges M. G. M. Verjans, Stipan Jonjic´, Jürgen Haas Attenuated and Replication-Competent Vaccinia Virus Strains Lucas Sánchez-Sampedro, Carmen 6955–6974 M65 and M101 with Distinct Biology and Immunogenicity as Elena Gómez, Ernesto Mejías-Pérez, Potential Vaccine Candidates against Pathogens Eva Pérez-Jiménez, Juan Carlos Oliveros, Mariano Esteban Inhibition of the HIV-1 Spike by Single-PG9/16-Antibody Robin Löving, Mathilda Sjöberg, 7000–7007 Binding Suggests a Coordinated-Activation Model for Its Three Shang-Rung Wu, James M. Binley, Protomeric Units Henrik Garoff Persistent Enteric Murine Norovirus Infection Is Associated Vesselin T. Tomov, Lisa C. Osborne, 7015–7031 with Functionally Suboptimal Virus-Specific CD8 T Cell Douglas V. Dolfi, Gregory F. Responses Sonnenberg, Laurel A. Monticelli, Kathleen Mansfield, Herbert W. Virgin, David Artis, E. John Wherry The Mature Virion of Ectromelia Virus, a Pathogenic Poxvirus, Xueying Ma, Ren-Huan Xu, Felicia 7046–7053 Is Capable of Intrahepatic Spread and Can Serve as a Target for Roscoe, J. Charles Whitbeck, Roselyn J. Delayed Therapy Eisenberg, Gary H. Cohen, Luis J. Sigal Suppressed Th17 Levels Correlate with Elevated PIAS3, SHP2, Sandra L. Bixler, Netanya G. Sandler, 7093–7101 and SOCS3 Expression in CD4 T Cells during Acute Simian Daniel C. Douek, Joseph J. Mattapallil Immunodeficiency Virus Infection

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HIV-1 Vpu Does Not Degrade Interferon Regulatory Factor 3 Dominik Hotter, Frank Kirchhoff, 7160–7165 Daniel Sauter ERRATUM Inhibition of Calmodulin-Dependent Kinase Kinase Blocks 7197 Human Cytomegalovirus-Induced Glycolytic Activation and Severely Attenuates Production of Viral Progeny AUTHOR’S CORRECTION Sequences in Gibbon Leukemia Virus Envelope That Confer 7198 Sensitivity to HIV-1 Accessory Protein Vpu

Cover photograph (Copyright © 2013, American Society for Microbiology. All Rights Reserved.): Age-dependent accumulation and mother- offspring transmission of simian foamy virus in wild chimpanzees. The background shows a mother chimpanzee (Pan troglodytes verus) grooming her offspring. Superimposed is a network of simian foamy virus (SFV) sequences isolated from individuals belonging to the same community (color code fits individual chimpanzees from which the sequences were derived). While horizontal transmission of SFV was previously seen as predominant, close mother-offspring relationships in wild chimpanzees result in frequent vertical retroviral transmission. Image by S. Metzger, Max Planck Institute for Evolutionary Anthropology, and K. Nowak, Robert Koch Institute. (See related article in May 2013, vol. 87, no. 9, p. 5193.)