Molecular Characterization of the Pseudorabies Virus UL2 Gene
Total Page:16
File Type:pdf, Size:1020Kb

Load more
Recommended publications
-
Cyprinus Carpio
Académie Universitaire Wallonie - Europe Université de Liège Faculté de Médecine Vétérinaire Département des Maladies Infectieuses et Parasitaires Service d’Immunologie et de Vaccinologie Etude des portes d’entrée de l’Herpèsvirus cyprin 3 chez Cyprinus carpio Study of the portals of entry of Cyprinid herpesvirus 3 in Cyprinus carpio Guillaume FOURNIER Thèse présentée en vue de l’obtention du grade de Docteur en Sciences Vétérinaires Année académique 2011-2012 Académie Universitaire Wallonie - Europe Université de Liège Faculté de Médecine Vétérinaire Département des Maladies Infectieuses et Parasitaires Service d’Immunologie et de Vaccinologie Etude des portes d’entrée de l’Herpèsvirus cyprin 3 chez Cyprinus carpio Study of the portals of entry of Cyprinid herpesvirus 3 in Cyprinus carpio Promoteur : Prof. Alain Vanderplasschen Guillaume FOURNIER Thèse présentée en vue de l’obtention du grade de Docteur en Sciences Vétérinaires Année académique 2011-2012 « La science progresse en indiquant l'immensité de l'ignoré. » Louis Pauwels Remerciements Liège, le 15 février 2012 L’accomplissement d’une thèse est un long et palpitant voyage en océan où se mélangent la curiosité, le doute, la persévérance, et la confiance… en soi bien sûr, mais surtout envers toutes les personnes qui, par leurs conseils, leur aide, leur soutien m’ont permis de mener cette thèse à bien. Je tiens ici à remercier mes collègues, amis et famille qui ont été tantôt les phares, tantôt les boussoles, toujours les fidèles compagnons de cette aventure. Je commencerais par adresser mes plus sincères remerciements à mon promoteur, le Professeur Alain Vanderplasschen, qui m’avait déjà remarqué en amphithéâtre pour ma curiosité, à moins que ce ne soit pour mon irrésistible coiffure.. -
Guide for Common Viral Diseases of Animals in Louisiana
Sampling and Testing Guide for Common Viral Diseases of Animals in Louisiana Please click on the species of interest: Cattle Deer and Small Ruminants The Louisiana Animal Swine Disease Diagnostic Horses Laboratory Dogs A service unit of the LSU School of Veterinary Medicine Adapted from Murphy, F.A., et al, Veterinary Virology, 3rd ed. Cats Academic Press, 1999. Compiled by Rob Poston Multi-species: Rabiesvirus DCN LADDL Guide for Common Viral Diseases v. B2 1 Cattle Please click on the principle system involvement Generalized viral diseases Respiratory viral diseases Enteric viral diseases Reproductive/neonatal viral diseases Viral infections affecting the skin Back to the Beginning DCN LADDL Guide for Common Viral Diseases v. B2 2 Deer and Small Ruminants Please click on the principle system involvement Generalized viral disease Respiratory viral disease Enteric viral diseases Reproductive/neonatal viral diseases Viral infections affecting the skin Back to the Beginning DCN LADDL Guide for Common Viral Diseases v. B2 3 Swine Please click on the principle system involvement Generalized viral diseases Respiratory viral diseases Enteric viral diseases Reproductive/neonatal viral diseases Viral infections affecting the skin Back to the Beginning DCN LADDL Guide for Common Viral Diseases v. B2 4 Horses Please click on the principle system involvement Generalized viral diseases Neurological viral diseases Respiratory viral diseases Enteric viral diseases Abortifacient/neonatal viral diseases Viral infections affecting the skin Back to the Beginning DCN LADDL Guide for Common Viral Diseases v. B2 5 Dogs Please click on the principle system involvement Generalized viral diseases Respiratory viral diseases Enteric viral diseases Reproductive/neonatal viral diseases Back to the Beginning DCN LADDL Guide for Common Viral Diseases v. -
Aujeszky's Disease
Aujeszky’s Disease Pseudorabies What is Aujeszky’s disease How does Aujeszky’s Can I get Aujeszky’s and what causes it? disease affect my animal? disease? Aujeszky’s disease, or pseudorabies, Disease may vary depending No. Signs of disease have not be is a contagious viral disease that on the age and species of animal reported in humans. primarily affects pigs. The virus causes affected; younger animals are the reproductive and severe neurological most severely affected. Piglets Who should I contact disease in affected animals; death is usually have a fever, stop eating, and if I suspect Aujeszky’s common. The disease occurs in parts show neurological signs (seizures, disease? of Europe, Southeast Asia, Central and paralysis), and often die within 24-36 Contact your veterinarian South America, and Mexico. It was hours. Older pigs may show similar immediately. Aujeszky’s disease is not once prevalent in the United States, symptoms, but often have respiratory currently found in domestic animals in but has been eradicated in commercial signs (coughing, sneezing, difficulty the United States; suspicion of disease operations; the virus is still found in breathing) and vomiting, are less likely requires immediate attention. feral (wild) swine populations. to die and generally recover in 5-10 days. Pregnant sows can abort or How can I protect my animal What animals get give birth to weak, trembling piglets. from Aujeszky’s disease? Aujeszky’s disease? Feral pigs do not usually show any Aujeszky’s disease is usually Pigs are the most frequently signs of disease. introduced into a herd from an affected animals, however nearly all Other animals usually die within a infected animal. -
Viral Diversity Among Different Bat Species That Share a Common Habitatᰔ Eric F
JOURNAL OF VIROLOGY, Dec. 2010, p. 13004–13018 Vol. 84, No. 24 0022-538X/10/$12.00 doi:10.1128/JVI.01255-10 Copyright © 2010, American Society for Microbiology. All Rights Reserved. Metagenomic Analysis of the Viromes of Three North American Bat Species: Viral Diversity among Different Bat Species That Share a Common Habitatᰔ Eric F. Donaldson,1†* Aimee N. Haskew,2 J. Edward Gates,2† Jeremy Huynh,1 Clea J. Moore,3 and Matthew B. Frieman4† Department of Epidemiology, University of North Carolina, Chapel Hill, North Carolina 275991; University of Maryland Center for Environmental Science, Appalachian Laboratory, Frostburg, Maryland 215322; Department of Biological Sciences, Oakwood University, Huntsville, Alabama 358963; and Department of Microbiology and Immunology, University of Maryland at Baltimore, Baltimore, Maryland 212014 Received 11 June 2010/Accepted 24 September 2010 Effective prediction of future viral zoonoses requires an in-depth understanding of the heterologous viral population in key animal species that will likely serve as reservoir hosts or intermediates during the next viral epidemic. The importance of bats as natural hosts for several important viral zoonoses, including Ebola, Marburg, Nipah, Hendra, and rabies viruses and severe acute respiratory syndrome-coronavirus (SARS-CoV), has been established; however, the large viral population diversity (virome) of bats has been partially deter- mined for only a few of the ϳ1,200 bat species. To assess the virome of North American bats, we collected fecal, oral, urine, and tissue samples from individual bats captured at an abandoned railroad tunnel in Maryland that is cohabitated by 7 to 10 different bat species. Here, we present preliminary characterization of the virome of three common North American bat species, including big brown bats (Eptesicus fuscus), tricolored bats (Perimyotis subflavus), and little brown myotis (Myotis lucifugus). -
THE ROLE of HERPESVIRUSES in MARINE TURTLE DISEASES By
THE ROLE OF HERPESVIRUSES IN MARINE TURTLE DISEASES By SADIE SHEA COBERLEY A DISSERTATION PRESENTED TO THE GRADUATE SCHOOL OF THE UNIVERSITY OF FLORIDA IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE DEGREE OF DOCTOR OF PHILOSOPHY UNIVERSITY OF FLORIDA 2002 Copyright 2002 by Sadie Shea Coberley For the turtles, and Carter and my family for encouraging me to pursue what I love. ACKNOWLEDGEMENTS I would like to thank my mentor, Dr. Paul Klein, for sharing his knowledge and for all of his encouragement and patience throughout my graduate education. He has been a true mentor in every sense of the word, and has done everything possible to prepare me for not only my scientific future, but phases of life outside of the laboratory as well. I would also like to thank my co-mentor, Dr. Rich Condit, first for seeing graduate student potential, and then for taking me in and helping to provide the necessary tools and expertise to cultivate it. In addition, I am indebted to Dr. Larry Herbst, who was not only my predecessor but a pioneer in FP research. His insight into studying such a complex problem has been invaluable. I am grateful for the critical analysis and raised eyebrow of Dr. Daniel Brown and for his assistance with trouble-shooting experiments, evaluating data, and preparing manuscripts. I am also appreciative of the assistance of Dr. Elliott Jacobson for including me in many discussions, necropsies, and analyses of marine turtles with interesting clinical signs of disease, and for sharing his vast knowledge of reptile diseases. I would like to thank Dr. -
Microtubule-Dependent Trafficking of Alphaherpesviruses in the Nervous
viruses Review Microtubule-Dependent Trafficking of Alphaherpesviruses in the Nervous System: The Ins and Outs Drishya Diwaker 1 and Duncan W. Wilson 1,2,* 1 Department of Developmental and Molecular Biology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA; [email protected] 2 Dominick P. Purpura Department of Neuroscience, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA * Correspondence: [email protected]; Tel.: +1-(718)-430-2305 Received: 29 November 2019; Accepted: 15 December 2019; Published: 17 December 2019 Abstract: The Alphaherpesvirinae include the neurotropic pathogens herpes simplex virus and varicella zoster virus of humans and pseudorabies virus of swine. These viruses establish lifelong latency in the nuclei of peripheral ganglia, but utilize the peripheral tissues those neurons innervate for productive replication, spread, and transmission. Delivery of virions from replicative pools to the sites of latency requires microtubule-directed retrograde axonal transport from the nerve terminus to the cell body of the sensory neuron. As a corollary, during reactivation newly assembled virions must travel along axonal microtubules in the anterograde direction to return to the nerve terminus and infect peripheral tissues, completing the cycle. Neurotropic alphaherpesviruses can therefore exploit neuronal microtubules and motors for long distance axonal transport, and alternate between periods of sustained plus end- and minus end-directed motion at different stages of their infectious cycle. This review summarizes our current understanding of the molecular details by which this is achieved. Keywords: herpes simplex virus; pseudorabies virus; neurons; anterograde axonal transport; retrograde axonal transport; microtubules; motors 1. -
Molecular Identification and Genetic Characterization of Cetacean Herpesviruses and Porpoise Morbillivirus
MOLECULAR IDENTIFICATION AND GENETIC CHARACTERIZATION OF CETACEAN HERPESVIRUSES AND PORPOISE MORBILLIVIRUS By KARA ANN SMOLAREK BENSON A THESIS PRESENTED TO THE GRADUATE SCHOOL OF THE UNIVERSITY OF FLORIDA IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE DEGREE OF MASTER OF SCIENCE UNIVERSITY OF FLORIDA 2005 Copyright 2005 by Kara Ann Smolarek Benson I dedicate this to my best friend and husband, Brock, who has always believed in me. ACKNOWLEDGMENTS First and foremost I thank my mentor, Dr. Carlos Romero, who once told me that love is fleeting but herpes is forever. He welcomed me into his lab with very little experience and I have learned so much from him over the past few years. Without his excellent guidance, this project would not have been possible. I thank my parents, Dave and Judy Smolarek, for their continual love and support. They taught me the importance of hard work and a great education, and always believed that I would be successful in life. I would like to thank Dr. Tom Barrett for the wonderful opportunity to study porpoise morbillivirus in his laboratory at the Institute for Animal Health in England, and Dr. Romero for making the trip possible. I especially thank Dr. Ashley Banyard for helping me accomplish all the objectives of the project, and all the wonderful people at the IAH for making a Yankee feel right at home in the UK. I thank Alexa Bracht and Rebecca Woodruff who have been with me in Dr. Romero’s lab since the beginning. Their continuous friendship and encouragement have kept me sane even in the most hectic of times. -
Monitoring of Pseudorabies in Wild Boar of Germany—A Spatiotemporal Analysis
pathogens Article Monitoring of Pseudorabies in Wild Boar of Germany—A Spatiotemporal Analysis Nicolai Denzin 1, Franz J. Conraths 1 , Thomas C. Mettenleiter 2 , Conrad M. Freuling 3 and Thomas Müller 3,* 1 Friedrich-Loeffler-Institut, Institute of Epidemiology, 17493 Greifswald-Insel Riems, Germany; Nicolai.Denzin@fli.de (N.D.); Franz.Conraths@fli.de (F.J.C.) 2 Friedrich-Loeffler-Institut, 17493 Greifswald-Insel Riems, Germany; Thomas.Mettenleiter@fli.de 3 Friedrich-Loeffler-Institut, Institute of Molecular Virology and Cell Biology, 17493 Greifswald-Insel Riems, Germany; Conrad.Freuling@fli.de * Correspondence: Thomas.Mueller@fli.de; Tel.: +49-38351-71659 Received: 16 March 2020; Accepted: 8 April 2020; Published: 10 April 2020 Abstract: To evaluate recent developments regarding the epidemiological situation of pseudorabies virus (PRV) infections in wild boar populations in Germany, nationwide serological monitoring was conducted between 2010 and 2015. During this period, a total of 108,748 sera from wild boars were tested for the presence of PRV-specific antibodies using commercial enzyme-linked immunosorbent assays. The overall PRV seroprevalence was estimated at 12.09% for Germany. A significant increase in seroprevalence was observed in recent years indicating both a further spatial spread and strong disease dynamics. For spatiotemporal analysis, data from 1985 to 2009 from previous studies were incorporated. The analysis revealed that PRV infections in wild boar were endemic in all German federal states; the affected area covers at least 48.5% of the German territory. There were marked differences in seroprevalence at district levels as well as in the relative risk (RR) of infection of wild boar throughout Germany. -
Where Do We Stand After Decades of Studying Human Cytomegalovirus?
microorganisms Review Where do we Stand after Decades of Studying Human Cytomegalovirus? 1, 2, 1 1 Francesca Gugliesi y, Alessandra Coscia y, Gloria Griffante , Ganna Galitska , Selina Pasquero 1, Camilla Albano 1 and Matteo Biolatti 1,* 1 Laboratory of Pathogenesis of Viral Infections, Department of Public Health and Pediatric Sciences, University of Turin, 10126 Turin, Italy; [email protected] (F.G.); gloria.griff[email protected] (G.G.); [email protected] (G.G.); [email protected] (S.P.); [email protected] (C.A.) 2 Complex Structure Neonatology Unit, Department of Public Health and Pediatric Sciences, University of Turin, 10126 Turin, Italy; [email protected] * Correspondence: [email protected] These authors contributed equally to this work. y Received: 19 March 2020; Accepted: 5 May 2020; Published: 8 May 2020 Abstract: Human cytomegalovirus (HCMV), a linear double-stranded DNA betaherpesvirus belonging to the family of Herpesviridae, is characterized by widespread seroprevalence, ranging between 56% and 94%, strictly dependent on the socioeconomic background of the country being considered. Typically, HCMV causes asymptomatic infection in the immunocompetent population, while in immunocompromised individuals or when transmitted vertically from the mother to the fetus it leads to systemic disease with severe complications and high mortality rate. Following primary infection, HCMV establishes a state of latency primarily in myeloid cells, from which it can be reactivated by various inflammatory stimuli. Several studies have shown that HCMV, despite being a DNA virus, is highly prone to genetic variability that strongly influences its replication and dissemination rates as well as cellular tropism. In this scenario, the few currently available drugs for the treatment of HCMV infections are characterized by high toxicity, poor oral bioavailability, and emerging resistance. -
Cyprinid Herpesvirus 3 Genesig Advanced
Primerdesign TM Ltd Cyprinid herpesvirus 3 Pol gene for DNA polymerase genesig® Advanced Kit 150 tests For general laboratory and research use only Quantification of Cyprinid herpesvirus 3 genomes. 1 genesig Advanced kit handbook HB10.03.11 Published Date: 09/11/2018 Introduction to Cyprinid herpesvirus 3 Cyprinid herpesvirus 3 (CyHV3) is the causative agent of a lethal disease in common (Cyprinus carpio carpio) and Koi carp (Cyprnius carpio koi). It was discovered in the late 1990s and has rapidly spread worldwide among cultured common carp and ornamental koi. Previously known as koi herpesvirus, it has caused severe economic losses in the global carp industry with its spread being attributed to international trade. The virus is a member of the order Herepesvirales and family Alloherpesviridae. It has a linear, double stranded genome of approximately 295 kb in length consisting of a large central portion flanked by two 22 kb repeat regions. The genome encodes 156 open reading frames (ORFs) including 8 ORFs encoded by the repeat regions. The genome is packaged in an icosahedral capsid that is contained within viral glycoproteins and then a host derived lipid envelope, giving an overall virion of 170-200nm in diameter. At present, common and koi carp are the only species known to be affected by the virus. The viral particles are transmitted through faeces, sloughing of mucous and inflammatory cells, and secretions that are released into the water. The skin pores are the main source of entry and site of replication but the disease also spreads to the organs, particularly the kidneys. The viral particles are further spread when the carp come into contact with each other during grazing, spawning or when uninfected fish pick at the skin lesions of dead infected fish. -
Topics in Viral Immunology Bruce Campell Supervisory Patent Examiner Art Unit 1648 IS THIS METHOD OBVIOUS?
Topics in Viral Immunology Bruce Campell Supervisory Patent Examiner Art Unit 1648 IS THIS METHOD OBVIOUS? Claim: A method of vaccinating against CPV-1 by… Prior art: A method of vaccinating against CPV-2 by [same method as claimed]. 2 HOW ARE VIRUSES CLASSIFIED? Source: Seventh Report of the International Committee on Taxonomy of Viruses (2000) Edited By M.H.V. van Regenmortel, C.M. Fauquet, D.H.L. Bishop, E.B. Carstens, M.K. Estes, S.M. Lemon, J. Maniloff, M.A. Mayo, D. J. McGeoch, C.R. Pringle, R.B. Wickner Virology Division International Union of Microbiological Sciences 3 TAXONOMY - HOW ARE VIRUSES CLASSIFIED? Example: Potyvirus family (Potyviridae) Example: Herpesvirus family (Herpesviridae) 4 Potyviruses Plant viruses Filamentous particles, 650-900 nm + sense, linear ssRNA genome Genome expressed as polyprotein 5 Potyvirus Taxonomy - Traditional Host range Transmission (fungi, aphids, mites, etc.) Symptoms Particle morphology Serology (antibody cross reactivity) 6 Potyviridae Genera Bymovirus – bipartite genome, fungi Rymovirus – monopartite genome, mites Tritimovirus – monopartite genome, mites, wheat Potyvirus – monopartite genome, aphids Ipomovirus – monopartite genome, whiteflies Macluravirus – monopartite genome, aphids, bulbs 7 Potyvirus Taxonomy - Molecular Polyprotein cleavage sites % similarity of coat protein sequences Genomic sequences – many complete genomic sequences, >200 coat protein sequences now available for comparison 8 Coat Protein Sequence Comparison (RNA) 9 Potyviridae Species Bymovirus – 6 species Rymovirus – 4-5 species Tritimovirus – 2 species Potyvirus – 85 – 173 species Ipomovirus – 1-2 species Macluravirus – 2 species 10 Higher Order Virus Taxonomy Nature of genome: RNA or DNA; ds or ss (+/-); linear, circular (supercoiled?) or segmented (number of segments?) Genome size – 11-383 kb Presence of envelope Morphology: spherical, filamentous, isometric, rod, bacilliform, etc. -
Emerging Viral Diseases of Fish and Shrimp Peter J
Emerging viral diseases of fish and shrimp Peter J. Walker, James R. Winton To cite this version: Peter J. Walker, James R. Winton. Emerging viral diseases of fish and shrimp. Veterinary Research, BioMed Central, 2010, 41 (6), 10.1051/vetres/2010022. hal-00903183 HAL Id: hal-00903183 https://hal.archives-ouvertes.fr/hal-00903183 Submitted on 1 Jan 2010 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés. Vet. Res. (2010) 41:51 www.vetres.org DOI: 10.1051/vetres/2010022 Ó INRA, EDP Sciences, 2010 Review article Emerging viral diseases of fish and shrimp 1 2 Peter J. WALKER *, James R. WINTON 1 CSIRO Livestock Industries, Australian Animal Health Laboratory (AAHL), 5 Portarlington Road, Geelong, Victoria, Australia 2 USGS Western Fisheries Research Center, 6505 NE 65th Street, Seattle, Washington, USA (Received 7 December 2009; accepted 19 April 2010) Abstract – The rise of aquaculture has been one of the most profound changes in global food production of the past 100 years. Driven by population growth, rising demand for seafood and a levelling of production from capture fisheries, the practice of farming aquatic animals has expanded rapidly to become a major global industry.