ORC3L (ORC3) Goat Polyclonal Antibody – TA302714 | Origene

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ORC3L (ORC3) Goat Polyclonal Antibody – TA302714 | Origene

OriGene Technologies, Inc. 9620 Medical Center Drive, Ste 200 Rockville, MD 20850, US Phone: +1-888-267-4436 [email protected] EU: [email protected] CN: [email protected] Product datasheet for TA302714 ORC3L (ORC3) Goat Polyclonal Antibody Product data: Product Type: Primary Antibodies Applications: WB Recommended Dilution: ELISA: 1:32,000. WB: 1-3µg/ml. Reactivity: Human (Expected from sequence similarity: Mouse, Rat, Dog, Cow) Host: Goat Isotype: IgG Clonality: Polyclonal Immunogen: Peptide with sequence QKTDHVARLTWGGC, from the C Terminus of the protein sequence according to NP_862820.1; NP_036513.2. Formulation: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Concentration: lot specific Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. Conjugation: Unconjugated Storage: Store at -20°C as received. Stability: Stable for 12 months from date of receipt. Gene Name: origin recognition complex subunit 3 Database Link: NP_036513 Entrez Gene 50793 MouseEntrez Gene 313138 RatEntrez Gene 474991 DogEntrez Gene 23595 Human Q9UBD5 This product is to be used for laboratory only. Not for diagnostic or therapeutic use. View online » ©2021 OriGene Technologies, Inc., 9620 Medical Center Drive, Ste 200, Rockville, MD 20850, US 1 / 2 ORC3L (ORC3) Goat Polyclonal Antibody – TA302714 Background: The origin recognition complex (ORC) is a highly conserved six subunits protein complex essential for the initiation of the DNA replication in eukaryotic cells. Studies in yeast demonstrated that ORC binds specifically to origins of replication and serves as a platform for the assembly of additional initiation factors such as Cdc6 and Mcm proteins. The protein encoded by this gene is a subunit of the ORC complex. Studies of a similar gene in Drosophila suggested a possible role of this protein in neuronal proliferation and olfactory memory. Alternatively spliced transcript variants encoding distinct isoforms have been reported for this gene. [provided by RefSeq] Synonyms: LAT; LATHEO; ORC3L Protein Pathways: Cell cycle Product images: TA302714 (2ug/ml) staining of Hela lysate (RIPA buffer, 1.4E5 cells per lane). Detected by western blot using chemiluminescence. This product is to be used for laboratory only. Not for diagnostic or therapeutic use. ©2021 OriGene Technologies, Inc., 9620 Medical Center Drive, Ste 200, Rockville, MD 20850, US 2 / 2.

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ORC3 (1D6): Sc-23888
SANTA CRUZ BIOTECHNOLOGY, INC. ORC3 (1D6): sc-23888 BACKGROUND STORAGE The initiation of DNA replication is a multi-step process that depends on the Store at 4° C, **DO NOT FREEZE**. Stable for one year from the date of formation of pre-replication complexes, which trigger initiation. Among the shipment. Non-hazardous. No MSDS required. proteins required for establishing these complexes are the origin recognition complex (ORC) proteins. ORC proteins bind specifically to origins of replication DATA where they serve as scaffold for the assembly of additional initiation factors. A B Human ORC subunits 1-6 are expressed in the nucleus of proliferating cells AB C DE and tissues, such as the testis. ORC1 and ORC2 are both expressed at equiv- 113 K – 89 K – alent concentrations throughout the cell cycle; however, only ORC2 remains < ORC3 stably bound to chromatin. ORC4 and ORC6 are also expressed constantly throughout the cell cycle. ORC2, ORC3, ORC4 and ORC5 form a core complex 49 K – upon which ORC6 and ORC1 assemble. The formation of this core complex suggests that ORC proteins play a crucial role in the G1-S transition in mam- malian cells. ORC3 (1D6): sc-23888. Western blot analysis of ORC3 ORC3 (1D6): sc-23888. Immunoperoxidase staining of expression in HeLa (A), Raji (B), Jurkat (C), WI-38 (D) formalin fixed, paraffin-embedded human ovary tissue CHROMOSOMAL LOCATION and A-549 (E) whole cell lysates. showing nuclear localization (A,B). Genetic locus: ORC3L (human) mapping to 6q15. SELECT PRODUCT CITATIONS SOURCE 1. Braden, W.A., et al. 2006. Distinct action of the retinoblastoma pathway ORC3 (1D6) is a rat monoclonal antibody raised against partially purified on the DNA replication machinery defines specific roles for cyclin-depen- His-tagged bacterially expressed fusion protein corresponding to human ORC3.
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Systemic Analysis of the DNA Replication Regulators Origin Recognition Complex in Lung Adenocarcinomas Identifes Prognostic and Expression Signifcance
Systemic Analysis of the DNA Replication Regulators Origin Recognition Complex in Lung Adenocarcinomas Identies Prognostic and Expression Signicance Juan Chen University of South China Juan Zou University of South China Juan Zeng University of South China Tian Zeng University of South China Qi-hao Hu University of South China Jun-hui Bai University of South China Min Tang University of South China Yu-kun Li ( [email protected] ) University of South China https://orcid.org/0000-0002-8517-9075 Primary research Keywords: lung adenocarcinomas, ORC complex, public databases, prognostic value, comprehensive bioinformatics Posted Date: May 11th, 2021 DOI: https://doi.org/10.21203/rs.3.rs-487176/v1 License: This work is licensed under a Creative Commons Attribution 4.0 International License. Read Full License Page 1/24 Abstract Background: Origin recognition complex (ORC) 1, ORC2, ORC3, ORC4, ORC5 and ORC6, form a replication- initiator complex to mediate DNA replication, which play a key role in carcinogenesis, while their role in lung adenocarcinomas (LUAD) remains poorly understood. Methods: We conrmed the transcriptional and post-transcriptional levels, DNA alteration, DNA methylation, miRNA network, protein structure, PPI network, functional enrichment, immune inltration and prognostic value of ORCs in LUAD based on Oncomine, GEPIA, HPA, cBioportal, TCGA, GeneMANIA, Metascape, KM-plot, GENT2, and TIMER database. Results: ORC mRNA and protein were both enhanced obviously based on Oncomine, Ualcan, GEPIA, TCGA and HPA database. Furthermore, ORC1 and ORC6 have signicant prognostic values for LUAD patients based on GEPIA database. Protein structure, PPI network, functional enrichment and immune inltration analysis indicated that ORC complex cooperatively accelerate the LUAD development by promoting DNA replication, cellular senescence and metabolic process.
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Chromosome Integrity in Saccharomyces Cerevisiae: the Interplay of DNA Replication Initiation Factors, Elongation Factors, and Origins
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Genome-Wide Analysis of the Core DNA Replication Machinery in the Higher Plants Arabidopsis and Rice1[W][OA]
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Human Origin Recognition Complex Is Essential for HP1 Binding to Chromatin and Heterochromatin Organization
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The Architecture of the DNA Replication Origin Recognition Complex in Saccharomyces Cerevisiae
View metadata, citation and similar papers at core.ac.uk brought to you by CORE provided by Spiral - Imperial College Digital Repository The architecture of the DNA replication origin recognition complex in Saccharomyces cerevisiae Zhiqiang Chen, Christian Speck, Patricia Wendel, Chunyan Tang, Bruce Stillman, and Huilin Li ABSTRACT The origin recognition complex (ORC) is conserved in all eukaryotes. The six proteins of the Saccharomyces cerevisiae ORC that form a stable complex bind to origins of DNA replication and recruit prereplicative complex (pre-RC) proteins, one of which is Cdc6. To further understand the function of ORC we recently determined by single-particle reconstruction of electron micrographs a low-resolution, 3D structure of S. cerevisiae ORC and the ORC–Cdc6 complex. In this article, the spatial arrangement of the ORC subunits within the ORC structure is described. In one approach, a maltose binding protein (MBP) was systematically fused to the N or the C termini of the five largest ORC subunits, one subunit at a time, generating 10 MBP-fused ORCs, and the MBP density was localized in the averaged, 2D EM images of the MBP-fused ORC particles. Determining the Orc1–5 structure and comparing it with the native ORC structure localized the Orc6 subunit near Orc2 and Orc3. Finally, subunit–subunit interactions were determined by immunoprecipitation of ORC subunits synthesized in vitro. Based on the derived ORC architecture and existing structures of archaeal Orc1–DNA structures, we propose a model for ORC and suggest how ORC interacts with origin DNA and Cdc6. The studies provide a basis for understanding the overall structure of the pre- RC.
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A Conserved but Plant-Specific CDK-Mediated Regulation of DNA Replication Protein A2 in the Precise Control of Stomatal Terminal Division
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Cshperspect-REP-A015727 Table3 1..10
Table 3. Nomenclature for proteins and protein complexes in different organisms Mammals Budding yeast Fission yeast Flies Plants Archaea Bacteria Prereplication complex assembly H. sapiens S. cerevisiae S. pombe D. melanogaster A. thaliana S. solfataricus E. coli Hs Sc Sp Dm At Sso Eco ORC ORC ORC ORC ORC [Orc1/Cdc6]-1, 2, 3 DnaA Orc1/p97 Orc1/p104 Orc1/Orp1/p81 Orc1/p103 Orc1a, Orc1b Orc2/p82 Orc2/p71 Orc2/Orp2/p61 Orc2/p69 Orc2 Orc3/p66 Orc3/p72 Orc3/Orp3/p80 Orc3/Lat/p82 Orc3 Orc4/p50 Orc4/p61 Orc4/Orp4/p109 Orc4/p52 Orc4 Orc5L/p50 Orc5/p55 Orc5/Orp5/p52 Orc5/p52 Orc5 Orc6/p28 Orc6/p50 Orc6/Orp6/p31 Orc6/p29 Orc6 Cdc6 Cdc6 Cdc18 Cdc6 Cdc6a, Cdc6b [Orc1/Cdc6]-1, 2, 3 DnaC Cdt1/Rlf-B Tah11/Sid2/Cdt1 Cdt1 Dup/Cdt1 Cdt1a, Cdt1b Whip g MCM helicase MCM helicase MCM helicase MCM helicase MCM helicase Mcm DnaB Mcm2 Mcm2 Mcm2/Nda1/Cdc19 Mcm2 Mcm2 Mcm3 Mcm3 Mcm3 Mcm3 Mcm3 Mcm4 Mcm4/Cdc54 Mcm4/Cdc21 Mcm4/Dpa Mcm4 Mcm5 Mcm5/Cdc46/Bob1 Mcm5/Nda4 Mcm5 Mcm5 Mcm6 Mcm6 Mcm6/Mis5 Mcm6 Mcm6 Mcm7 Mcm7/Cdc47 Mcm7 Mcm7 Mcm7/Prolifera Gmnn/Geminin Geminin Mcm9 Mcm9 Hbo1 Chm/Hat1 Ham1 Ham2 DiaA Ihfa Ihfb Fis SeqA Replication fork assembly Hs Sc Sp Dm At Sso Eco Mcm8 Rec/Mcm8 Mcm8 Mcm10 Mcm10/Dna43 Mcm10/Cdc23 Mcm10 Mcm10 DDK complex DDK complex DDK complex DDK complex Cdc7 Cdc7 Hsk1 l(1)G0148 Hsk1-like 1 Dbf4/Ask Dbf4 Dfp1/Him1/Rad35 Chif/chiffon Drf1 Continued 2 Replication fork assembly (Continued ) Hs Sc Sp Dm At Sso Eco CDK complex CDK complex CDK complex CDK complex CDK complex Cdk1 Cdc28/Cdk1 Cdc2/Cdk1 Cdc2 CdkA Cdk2 Cdc2c CcnA1, A2 CycA CycA1, A2,
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