Spiny Mice (Acomys) Exhibit Attenuated Hallmarks of Aging And
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Study for Pathogenesis of Congenital Cholesteatoma with Comparison of Proteins Expressed in Congenital Cholesteatoma, Acquired C
Study for pathogenesis of congenital cholesteatoma with comparison of proteins expressed in congenital cholesteatoma, acquired cholesteatoma and skin of the external auditory canal through proteomics Seung Ho Shin Department of Medicine The Graduate School, Yonsei University Study for pathogenesis of congenital cholesteatoma with comparison of proteins expressed in congenital cholesteatoma, acquired cholesteatoma and skin of the external auditory canal through proteomics Directed by Professor Jae Young Choi The Doctoral Dissertation submitted to the Department of Medicine, the Graduate School of Yonsei University in partial fulfillment of the requirements for the degree of Doctor of Philosophy Seung Ho Shin June 2014 ACKNOWLEDGEMENTS In the initial period of my fellowship, I wrote a book, Temporal Bone Dissection Manual as a coauthor with Professor Won Sang Lee, Ho-Ki Lee and Jae Young Choi. Through this book, I learned about much knowledge from them. Professor Jae Young Choi advised me to go for a Ph.D. I admitted graduate school for a Ph.D. in 2007. In my doctoral course, he has instructed me in detail on the basic research. He has demonstrated precise and delicate laboratory techniques and showed outstanding ability to create new ideas. Also, he has often said to me that a researcher must be honest to his colleagues and even to himself. After the summer of 2013, he gave me an idea for this paper, which was for congenital cholesteatoma analysis with proteomics. He always displayed endless energy and enthusiasm for scientific experiments even after many demanding surgeries. His passion motivated me to follow suit and seven months of our work at last bore fruit. -
<I>Acomys Cahirinus</I>
Journal of the American Association for Laboratory Animal Science Vol 55, No 1 Copyright 2016 January 2016 by the American Association for Laboratory Animal Science Pages 9–17 The Biology and Husbandry of the African Spiny Mouse (Acomys cahirinus) and the Research Uses of a Laboratory Colony Cheryl L Haughton,1,† Thomas R Gawriluk,2,† and Ashley W Seifert2,* African spiny mice (Acomys spp.) are unique precocial rodents that are found in Africa, the Middle East, and southern Asia. They exhibit several interesting life-history characteristics, including precocial development, communal breeding, and a suite of physiologic adaptations to desert life. In addition to these characteristics, African spiny mice are emerging as an important animal model for tissue regeneration research. Furthermore, their important phylogenetic position among murid rodents makes them an interesting model for evolution and development studies. Here we outline the necessary components for maintaining a successful captive breeding colony, including laboratory housing, husbandry, and health monitoring as- pects. We also review past and present studies focused on spiny mouse behavior, reproduction, and disease. Last, we briefly summarize various current biomedical research directions using captive-bred spiny mice. Rodents of the genus Acomys are collectively referred to as Taxonomy and Unique Properties ‘spiny mice’ due to the prominent spiny hairs that emerge Acomys spp. are members of the family Muridae, a taxonomic 57 from their dorsal skin. Acomys takes its name -
(Acomys Cahirinus) Betsy Peitz Biology Department, Case Western Reserve University, Cleveland, Ohio 44106, U.SA
The oestrous cycle of the spiny mouse (Acomys cahirinus) Betsy Peitz Biology Department, Case Western Reserve University, Cleveland, Ohio 44106, U.SA. Summary. The oestrous cycle of the spiny mouse (Acomys cahirinus), as determined by vaginal smears, is 11\m=.\1\m=+-\1\m=.\9(s.d.) days (n = 110). The vaginal smears show cell patterns similar to those seen in the rat, but secretion of mucus is greater than in the rat. The average age at vaginal opening is 45 \m=+-\2\m=.\77(s.e.m.) days, but the first litter (sired by litter mates) did not occur until 103 \m=+-\4\m=.\04(s.e.m.) days. The decidual response to uterine trauma indicates that there is an active luteal phase. The ovaries are otherwise histologically similar to those of other murids. Introduction The spiny mouse (Acomys cahirinus) is a desert-dwelling murid rodent found in Egypt, Israel and other areas of the Middle East. They are nocturnal animals with peaks of activity at dawn and dusk (Bodenheimer, 1949). The species has frequently been used for studies of renal physiology because its kidneys are able to concentrate urine to 4-8 M and because it conserves plasma volume during dehydration (Shkolnik & Borut, 1969; Horowitz & Borut, 1970; Borut, Horowitz & Castel, 1972). They have also been used for studies of diabetes and obesity (Strasser, 1968; Hefti & Fluckiger, 1967; Pictet, Orci, Gonet, Rouiller & Renold, 1967; Gonet, Stauffacher, Pictet & Renold, 1965; Cameron, Stauffacher, Orci, Amherdt & Renold, 1972). Most of these studies on diabetes were carried out on colonies derived from one established in Basel (Young, 1976) but diabetes may not be widespread. -
Supplementary Material Contents
Supplementary Material Contents Immune modulating proteins identified from exosomal samples.....................................................................2 Figure S1: Overlap between exosomal and soluble proteomes.................................................................................... 4 Bacterial strains:..............................................................................................................................................4 Figure S2: Variability between subjects of effects of exosomes on BL21-lux growth.................................................... 5 Figure S3: Early effects of exosomes on growth of BL21 E. coli .................................................................................... 5 Figure S4: Exosomal Lysis............................................................................................................................................ 6 Figure S5: Effect of pH on exosomal action.................................................................................................................. 7 Figure S6: Effect of exosomes on growth of UPEC (pH = 6.5) suspended in exosome-depleted urine supernatant ....... 8 Effective exosomal concentration....................................................................................................................8 Figure S7: Sample constitution for luminometry experiments..................................................................................... 8 Figure S8: Determining effective concentration ......................................................................................................... -
Monkeys, Mice and Menses: the Bloody Anomaly of the Spiny Mouse
Journal of Assisted Reproduction and Genetics (2019) 36:811–817 https://doi.org/10.1007/s10815-018-1390-3 COMMENTARY Monkeys, mice and menses: the bloody anomaly of the spiny mouse Nadia Bellofiore1,2 & Jemma Evans3 Received: 18 November 2018 /Accepted: 17 December 2018 /Published online: 5 January 2019 # Springer Science+Business Media, LLC, part of Springer Nature 2019 Abstract The common spiny mouse (Acomys cahirinus) is the only known rodent to demonstrate a myriad of physiological processes unseen in their murid relatives. The most recently discovered of these uncharacteristic traits: spontaneous decidual transformation of the uterus in virgin females, preceding menstruation. Menstruation occurring without experimental intervention in rodents has not been documented elsewhere to date, and natural menstruation is indeed rare in the animal kingdom outside of higher order primates. This review briefly summarises the current knowledge of spiny mouse biology and taxonomy, and explores their endocrinology which may aid in our understanding of the evolution of menstruation in this species. We propose that DHEA, synthesised by the spiny mouse (but not other rodents), humans and other menstruating primates, is integral in spontaneous decidualisation and therefore menstruation. We discuss both physiological and behavioural attributes across the menstrual cycle in the spiny mouse analogous to those observed in other menstruating species, including premenstrual syndrome. We further encourage the use of the spiny mouse as a small animal model of menstruation and female reproductive biology. Keywords Menstruation . Novel model . Evolution Introduction ovulation (for comprehensive review of domestic animal oestrous cycles, see [7]); rather, ovulation is spontaneous Despite the (quite literal) billions of women worldwide under- and occurs cyclically throughout the year. -
Keratin 1 Maintains Skin Integrity and Participates in an Inflammatory
Research Article 5269 Keratin 1 maintains skin integrity and participates in an inflammatory network in skin through interleukin-18 Wera Roth1, Vinod Kumar1, Hans-Dietmar Beer2, Miriam Richter1, Claudia Wohlenberg3, Ursula Reuter3, So¨ ren Thiering1, Andrea Staratschek-Jox4, Andrea Hofmann4, Fatima Kreusch4, Joachim L. Schultze4, Thomas Vogl5, Johannes Roth5, Julia Reichelt6, Ingrid Hausser7 and Thomas M. Magin1,* 1Translational Centre for Regenerative Medicine (TRM) and Institute of Biology, University of Leipzig, 04103 Leipzig, Germany 2University Hospital, Department of Dermatology, University of Zurich, 8006 Zurich, Switzerland 3Institute of Biochemistry and Molecular Biology, Division of Cell Biochemistry, University of Bonn, 53115 Bonn, Germany 4Department of Genomics and Immunoregulation, LIMES Institute, University of Bonn, 53115 Bonn, Germany 5Institute of Immunology, University of Mu¨nster, 48149 Mu¨nster, Germany 6Institute of Cellular Medicine and North East England Stem Cell Institute, Newcastle University, Newcastle upon Tyne NE2 4HH, UK 7Universita¨ts-Hautklinik, Ruprecht-Karls-Universita¨t Heidelberg, 69120 Heidelberg, Germany *Author for correspondence ([email protected]) Accepted 8 October 2012 Journal of Cell Science 125, 5269–5279 ß 2012. Published by The Company of Biologists Ltd doi: 10.1242/jcs.116574 Summary Keratin 1 (KRT1) and its heterodimer partner keratin 10 (KRT10) are major constituents of the intermediate filament cytoskeleton in suprabasal epidermis. KRT1 mutations cause epidermolytic ichthyosis in humans, characterized by loss of barrier integrity and recurrent erythema. In search of the largely unknown pathomechanisms and the role of keratins in barrier formation and inflammation control, we show here that Krt1 is crucial for maintenance of skin integrity and participates in an inflammatory network in murine keratinocytes. -
Retinoic Acid and Its 4-Oxo Metabolites Are Functionally Active in Human Skin Cells in Vitro
View metadata, citation and similar papers at core.ac.uk brought to you by CORE provided by Elsevier - Publisher Connector Retinoic Acid and its 4-Oxo Metabolites are Functionally Active in Human Skin Cells In Vitro Jens M. Baron,Ã,1 Ruth Heise,Ã,1 William S. Blaner,w Mark Neis,Ã Sylvia Joussen,Ã Alexandra Dreuw,z Yvonne Marquardt,Ã Jean-Hilaire Saurat,y Hans F. Merk,Ã David R. Bickers,z and Frank K. Jugertw ÃDepartment of Dermatology and Allergology, University Hospital of the RWTH, Aachen, Germany; wDepartment of Medicine, Columbia University, College of Physicians and Surgeons, New York, New York, USA; zInstitute of Biochemistry, University Hospital of the RWTH, Aachen, Germany; yDepartment of Dermatology, Hoˆ pital Cantonal University, Geneva, Switzerland; zDepartment of Dermatology, Columbia University, College of Physicians and Surgeons, New York, New York, USA Retinoic acid exerts a variety of effects on gene transcription that regulate growth, differentiation, and inflammation in normal and neoplastic skin cells. Because there is a lack of information regarding the influence of metabolic transformation of retinoids on their pharmacologic effects in skin, we have analyzed the functional activity of all- trans-, 9-cis-, and 13-cis-retinoic acid and their 4-oxo-metabolites in normal human epidermal keratinocytes (NHEKs) and dermal fibroblasts using gene and protein expression profiling techniques, including cDNA microar- rays, two-dimensional gel electrophoresis, and MALDI-MS. It was previously thought that the 4-oxo-metabolites of RA are inert catabolic end-products but our results indicate instead that they display strong and isomer-specific transcriptional regulatory activity in both NHEKs and dermal fibroblasts. -
University of Groningen Epidermolysis Bullosa Simplex Bolling, Maria
University of Groningen Epidermolysis bullosa simplex Bolling, Maria Caroline IMPORTANT NOTE: You are advised to consult the publisher's version (publisher's PDF) if you wish to cite from it. Please check the document version below. Document Version Publisher's PDF, also known as Version of record Publication date: 2010 Link to publication in University of Groningen/UMCG research database Citation for published version (APA): Bolling, M. C. (2010). Epidermolysis bullosa simplex: new insights in desmosomal cardiocutaneous syndromes. s.n. Copyright Other than for strictly personal use, it is not permitted to download or to forward/distribute the text or part of it without the consent of the author(s) and/or copyright holder(s), unless the work is under an open content license (like Creative Commons). Take-down policy If you believe that this document breaches copyright please contact us providing details, and we will remove access to the work immediately and investigate your claim. Downloaded from the University of Groningen/UMCG research database (Pure): http://www.rug.nl/research/portal. For technical reasons the number of authors shown on this cover page is limited to 10 maximum. Download date: 25-09-2021 9 Discussion and future perspectives MC Bolling Center for Blistering Diseases, Department of Dermatology, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands Chapter 9 190 Discussion and future perspectives Genotype-phenotype correlation of mutations in the genes encoding the basal and suprabasal keratins Epidermolysis bullosa simplex (EBS) was the first hereditary skin blistering disorder of which the etiology was established in the early 1990s.1-3 EBS was also the first genetic disease caused by mutations in genes encoding intermediate filament (IF) proteins, namely the basal epidermal keratins K5 and K14. -
Mutations Affecting Keratin 10 Surface-Exposed Residues Highlight The
ORIGINAL ARTICLE Mutations Affecting Keratin 10 Surface-Exposed Residues Highlight the Structural Basis of Phenotypic Variation in Epidermolytic Ichthyosis Haris Mirza1, Anil Kumar2, Brittany G. Craiglow1, Jing Zhou1, Corey Saraceni1, Richard Torbeck3, Bruce Ragsdale4, Paul Rehder5, Annamari Ranki6 and Keith A. Choate1,7 Epidermolytic ichthyosis (EI) due to KRT10 mutations is a rare, typically autosomal dominant, disorder characterized by generalized erythema and cutaneous blistering at birth followed by hyperkeratosis and less frequent blistering later in life. We identified two KRT10 mutations p.Q434del and p.R441P in subjects presenting with a mild EI phenotype. Both occur within the mutational “hot spot” of the keratin 10 (K10) 2B rod domain, adjacent to severe EI-associated mutations. p.Q434del and p.R441P formed collapsed K10 fibers rather than aggregates characteristic of severe EI KRT10 mutations such as p.R156C. Upon differentiation, keratinocytes from p.Q434del showed significantly lower apoptosis (P-valueo0.01) compared with p.R156C as assessed by the TUNEL assay. Conversely, the mitotic index of the p.Q434del epidermis was significantly higher compared with that of p.R156C (P-valueo0.01) as estimated by the Ki67 assay. Structural basis of EI phenotype variation was investigated by homology-based modeling of wild-type and mutant K1–K10 dimers. Both mild EI mutations were found to affect the surface-exposed residues of the K10 alpha helix coiled-coil and caused localized disorganization of the K1–K10 heterodimer. In contrast, adjacent severe EI mutations disrupt key intermolecular dimer interactions. Our findings provide structural insights into phenotypic variation in EI due to KRT10 mutations. -
The Spiny Mouse, Acomys Cahirinus Malcolm Maden* and Justin A
© 2020. Published by The Company of Biologists Ltd | Development (2020) 147, dev167718. doi:10.1242/dev.167718 PRIMER Model systems for regeneration: the spiny mouse, Acomys cahirinus Malcolm Maden* and Justin A. Varholick ABSTRACT Voronstova and Liosner, 1960) and perhaps also chinchillas, cows The spiny mouse, Acomys spp., is a recently described model and pigs (Williams-Boyce and Daniel, 1986). It is also known that organism for regeneration studies. For a mammal, it displays several individual mammalian tissues can regenerate, such as surprising powers of regeneration because it does not fibrose (i.e. skeletal muscle after myotoxin administration (Musarò, 2014) and scar) in response to tissue injury as most other mammals, including the liver, which displays prodigious powers of proliferation during humans, do. In this Primer article, we review these regenerative compensatory hypertrophy (Fausto et al., 2012). This compensatory abilities, highlighting the phylogenetic position of the spiny mouse hypertrophy is a process which the lungs can also undergo (Hsia, relative to other rodents. We also briefly describe the Acomys tissues 2017), whereby the tissue remaining after removal of part of the that have been used for regeneration studies and the common organ expands to compensate for the missing part. Many features of their regeneration compared with the typical mammalian mammalian epithelial tissues, such as the epidermis or the response. Finally, we discuss the contribution that Acomys has made intestinal lining, also exhibit continuous replacement, although in understanding the general principles of regeneration and elaborate this is a property of all animals and so is not considered an unusual hypotheses as to why this mammal is successful at regenerating. -
Culturing Keratinocytes on Biomimetic Substrates Facilitates Improved Epidermal Assembly in Vitro
cells Article Culturing Keratinocytes on Biomimetic Substrates Facilitates Improved Epidermal Assembly In Vitro Eve Hunter-Featherstone 1, Natalie Young 1, Kathryn Chamberlain 1, Pablo Cubillas 2, Ben Hulette 3, Xingtao Wei 3, Jay P. Tiesman 3, Charles C. Bascom 3, Adam M. Benham 1 , Martin W. Goldberg 1 , Gabriele Saretzki 4 and Iakowos Karakesisoglou 1,* 1 Department of Biosciences, Durham University, Durham DH1 3LE, UK; [email protected] (E.H.-F.); [email protected] (N.Y.); [email protected] (K.C.); [email protected] (A.M.B.); [email protected] (M.W.G.) 2 Department of Earth Sciences, Durham University, Durham DH1 3LE, UK; [email protected] 3 The Procter & Gamble Company, Cincinnati, OH 45202, USA; [email protected] (B.H.); [email protected] (X.W.); [email protected] (J.P.T.); [email protected] (C.C.B.) 4 Biosciences Institute, Newcastle University, Newcastle-upon-Tyne NE1 7RU, UK; [email protected] * Correspondence: [email protected] Abstract: Mechanotransduction is defined as the ability of cells to sense mechanical stimuli from their surroundings and translate them into biochemical signals. Epidermal keratinocytes respond to mechanical cues by altering their proliferation, migration, and differentiation. In vitro cell culture, Citation: Hunter-Featherstone, E.; however, utilises tissue culture plastic, which is significantly stiffer than the in vivo environment. Cur- Young, N.; Chamberlain, K.; Cubillas, rent epidermal models fail to consider the effects of culturing keratinocytes on plastic prior to setting P.; Hulette, B.; Wei, X.; Tiesman, J.P.; up three-dimensional cultures, so the impact of this non-physiological exposure on epidermal assem- Bascom, C.C.; Benham, A.M.; bly is largely overlooked. -
Systematics, Distribution and Ecological Analysis of Rodents in Jordan
Systematics, distribution and ecological analysis of rodents in Jordan ZUHAIR S. AMR1,2, MOHAMMAD A. ABU BAKER3, MAZIN QUMSIYEH4 & EHAB EID5 1Department of Biology, Jordan University of Science and Technology, P. O. Box 3030, Irbid, Jordan. E-mail: [email protected] 2Corresponding author 2Enviromatics, Environmental Management and Consulting Company, Amman, Jordan, E- mail: [email protected] 3Palestine Museum of Natural History, Bethlehem University, Bethlehem, Palestine, E-mail: [email protected]. 4The Royal Marine Conservation Society of Jordan, Amman, Jordan, E-mail: [email protected] Abstract Distributional and ecological data were given to all rodents of Jordan. The rodent fauna of Jordan consists of 28 species with 20 genera in eight families (Cricetidae, Dipodidae, Gliridae, Hystricidae, Muridae, Myocastoridae, Sciuridae, and Spalacidae), including four introduced species. Keys for families and species were provided, along with diagnosis for each species and cranial illustrations for most species. Habitat preference and zoogeographic affinities of rodents in Jordan were analyzed, as well as their status and conservation. Threat categories and causes of threats on the rodents of Jordan were also analyzed. The distribution of rodents in Jordan represents a reflection of their global distribution ranges and habitat preferences. Species associated with the temperate forest of northern Jordan includes Sciurus anomalus and two wood mice, Apodemus mystacinus and A. flavicollis, while non-forested areas are represented by Nannospalax ehrenbergi and Microtus guentheri. Strict sand dwellers include Gerbillus cheesmani and G. gerbillus. Petrophiles associated with sandstone or black lava deserts are exemplified by Acomys russatus, A. r. lewsi, H. indica and S. calurus. Others including: Jaculus jaculus, G.