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Monoclonal Antibody to LPP - Ascites

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Monoclonal Antibody to LPP - Ascites OriGene Technologies, Inc. OriGene Technologies GmbH 9620 Medical Center Drive, Ste 200 Schillerstr. 5 Rockville, MD 20850 32052 Herford UNITED STATES GERMANY Phone: +1-888-267-4436 Phone: +49-5221-34606-0 Fax: +1-301-340-8606 Fax: +49-5221-34606-11 [email protected] [email protected] AM06208SU-N Monoclonal Antibody to LPP - Ascites Alternate names: LIM domain-containing preferred translocation partner in lipoma, LPP, Lipoma- preferred partner Quantity: 0.1 ml Background: LIM domain containing preferred translocation partner in lipoma. The Zyxin family of proteins contains five members, Ajuba, LIMD1, LPP,TRIP6 and Zyxin. LPP (LIM- containing lipoma-preferred partner), a LIM domain-containing scaffolding protein contains three LIM domains at its carboxyterminus, which are preceded by a proline- rich pre-LIM region containing a number of protein interaction domains. LPP, an 80 kDa protein, localizes to sites of cell adhesion, such as focal adhesions and cell-cell contacts,and shuttles to the nucleus where it has transcriptional activation capacity.The human LPP gene maps to chromosomal location 3q28, and preferentiallytranslocates to the HMGIC gene in a subclass of human benign mesenchymal tumors known as lipomas. Uniprot ID: Q93052 NCBI: NP_001161143.1 GeneID: 4026 Host / Isotype: Mouse / IgG1 Clone: 8B3A11 Immunogen: Purified recombinant fragment of Human LPP expressed in E. Coli. Format: State: Ascitic fluid Preservatives: 0.03% Sodium Azide Applications: ELISA: 1/10000. Western Blotting: 1/500-1/2000. Immunofluorescence: 1/200-1/1000. Immunohistochemistry on Paraffin Sections: 1/200-1/1000. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user. Molecular Weight: 66 kDa Specificity: Recognizes LIM Protein / LPP. Species Reactivity: Tested: Human, Mouse. Monkey and Hamster. Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. General Readings: 1. BMC Cell Biol. 2005 Jan 13;6(1):1 2. Zhang Y, Wolf-Yadlin A, Ross PL, Pappin DJ, Rush J, Lauffenburger DA, et al. Time- resolved mass spectrometry of tyrosine phosphorylation sites in the epidermal growth factor receptor signaling network reveals dynamic modules. Mol Cell Proteomics. 2005 Sep;4(9):1240-50. Epub 2005 Jun 11. PubMed PMID: 15951569. For research and in vitro use only. Not for diagnostic or therapeutic work. Material Safety Datasheets are available at www.acris-antibodies.com or on request. MS/20130628 1 / 3 AM06208SU-N: Monoclonal Antibody to LPP - Ascites 3. Cancer Genet Cytogenet. 2005 Nov;163(1):68-70. Pictures: Immunohistochemical analysis of paraffin-embedded human small intestine using anti-LPP Monoclonal Antibody with DAB staining. Immunohistochemical analysis of paraffin-embedded human vessels tissues using anti-LPP Monoclonal Antibody Confocal immunofluorescence analysis of COS cells using anti-LPP Monoclonal Antibody (green). Red: Actin filaments have been labeled using DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye. Confocal immunofluorescence analysis of Hela cells using anti-LPP Monoclonal Antibody (green). Red: Actin filaments have been labeled using DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye. For research and in vitro use only. Not for diagnostic or therapeutic work. Material Safety Datasheets are available at www.acris-antibodies.com or on request. MS/20130628 2 / 3 AM06208SU-N: Monoclonal Antibody to LPP - Ascites Western blot analysis using anti-LPP Monoclonal Antibody against Hela (Lane 1), NIH/3T3 (Lane 2), COS (Lane 3), Caki (Lane 4), MCF-7 (Lane 5), HepG2 (Lane 6) and SMMC-7721 (Lane 7) cell lysate. For research and in vitro use only. Not for diagnostic or therapeutic work. Material Safety Datasheets are available at www.acris-antibodies.com or on request. MS/20130628 3 / 3 Powered by TCPDF (www.tcpdf.org).
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