ALOXE3 Rabbit Pab

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Leader in Biomolecular Solutions for Life Science ALOXE3 Rabbit pAb Catalog No.: A8245 Basic Information Background Catalog No. This gene is a member of the lipoxygenase family, which are catabolized by arachidonic A8245 acid-derived compounds. The encoded enzyme is a hydroperoxide isomerase that synthesizes a unique type of epoxy alcohol (8R-hydroxy-11R,12R- Observed MW epoxyeicosa-5Z,9E,14Z-trienoic acid) from 12R-hydroperoxyeicosatetraenoic acid (12R- 81kDa HPETE). This epoxy alcohol can activate the the nuclear receptor peroxisome proliferator-activated receptor alpha (PPARalpha), which is implicated in epidermal Calculated MW differentiation. Loss of function of the enzyme encoded by this gene results in ichthyosis, 80kDa/95kDa implicating the function of this gene in the differentiation of human skin. This gene is part of a cluster of lipoxygenase genes on 17p13.1. Mutations in this gene result in Category nonbullous congenital ichthyosiform erythroderma (NCIE). Multiple transcript variants encoding different isoforms have been found for this gene. Primary antibody Applications WB Cross-Reactivity Human, Mouse, Rat Recommended Dilutions Immunogen Information WB 1:500 - 1:2000 Gene ID Swiss Prot 59344 Q9BYJ1 Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human ALOXE3 (NP_067641.2). Synonyms ALOXE3;ARCI3;E-LOX;eLOX-3;eLOX3 Contact Product Information www.abclonal.com Source Isotype Purification Rabbit IgG Affinity purification Storage Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3. Validation Data Western blot analysis of extracts of various cell lines, using ALOXE3 antibody (A8245) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. Antibody | Protein | ELISA Kits | Enzyme | NGS | Service For research use only. Not for therapeutic or diagnostic purposes. Please visit http://abclonal.com for a complete listing of recommended products..

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Meta-Analysis of Mutations in ALOX12B Or ALOXE3 Identified in a Large Cohort of 224 Patients
University of Groningen Meta-Analysis of Mutations in ALOX12B or ALOXE3 Identified in a Large Cohort of 224 Patients Hotz, Alrun; Kopp, Julia; Bourrat, Emmanuelle; Oji, Vinzenz; Komlosi, Katalin; Giehl, Kathrin; Bouadjar, Bakar; Bygum, Anette; Tantcheva-Poor, Iliana; Hellstrom Pigg, Maritta Published in: Genes DOI: 10.3390/genes12010080 IMPORTANT NOTE: You are advised to consult the publisher's version (publisher's PDF) if you wish to cite from it. Please check the document version below. Document Version Publisher's PDF, also known as Version of record Publication date: 2021 Link to publication in University of Groningen/UMCG research database Citation for published version (APA): Hotz, A., Kopp, J., Bourrat, E., Oji, V., Komlosi, K., Giehl, K., Bouadjar, B., Bygum, A., Tantcheva-Poor, I., Hellstrom Pigg, M., Has, C., Yang, Z., Irvine, A. D., Betz, R. C., Zambruno, G., Tadini, G., Suessmuth, K., Gruber, R., Schmuth, M., ... Fischer, J. (2021). Meta-Analysis of Mutations in ALOX12B or ALOXE3 Identified in a Large Cohort of 224 Patients. Genes, 12(1), [80]. https://doi.org/10.3390/genes12010080 Copyright Other than for strictly personal use, it is not permitted to download or to forward/distribute the text or part of it without the consent of the author(s) and/or copyright holder(s), unless the work is under an open content license (like Creative Commons). Take-down policy If you believe that this document breaches copyright please contact us providing details, and we will remove access to the work immediately and investigate your claim. Downloaded from the University of Groningen/UMCG research database (Pure): http://www.rug.nl/research/portal.
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Supporting Information
Supporting Information Yang et al. 10.1073/pnas.1603244113 A Cell line = BJeLR cells ALOX5 ALOX12 ALOX12B Ct = N.D. Ct = N.D. Ct = 34.434 ALOX15 ALOX15B ALOXE3 Ct = 30.711 Ct = 34.132 Ct = 28.326 Cell line = HT-1080 cells ALOX5 ALOX12 ALOX12B Ct = N.D. Ct = N.D. Ct = 35.46 ALOX15 ALOX15B ALOXE3 Ct = N.D. Ct = 34.64 Ct = 31.137 B Cell line = HT-1080 cells 1.5 1.5 1.0 1.0 0.5 0.5 ALOX15B mRNA level ALOX15B expression) (relative ALOX15B mRNA level ALOX15B expression) (relative 0.0 0.0 siNeg siNeg siALOX15B siALOXE3 Fig. S1. (A) Expression analysis of ALOX genes in BJeLR and HT-1080 cells. The figures show the amplification plot of each ALOX isoform. Triplicate samples were analyzed for each gene using mRNA preparation from BJeLR cells. The red lines in each plot indicate ACTB gene amplification that served as endogenous control in the quantification. The gene name and the Ct number, if was possible to determine, are presented. A Ct value greater than 35 is considered a weak expression level, which suggests that ALOXE3 is the major isoform expressed in these cell lines. N.D., not determined. (B) Knockdown of ALOX15B and ALOXE3 expression by the pool of siRNAs was confirmed using qPCR analysis. Data are presented as mean ± SD; n = 3. Yang et al. www.pnas.org/cgi/content/short/1603244113 1of3 GFP-ALOX5 location n.s. BJeHBJeH BJeHLTBJeHLT BJeLRBJeLR 50 40 DMSO 30 20 10 0 Translocated cells per field cells Translocated (IONO group) BJeH BJeLR BJeHLT [IONO], 113 μM [IONO], BJeHBJeH BJeHLTBJeHLT BJeLRBJeLR Ferroptosis Ferroptosis Resistant Sensitive Fig.
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