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Training Manual Series No.15/2018
View metadata, citation and similar papers at core.ac.uk brought to you by CORE provided by CMFRI Digital Repository DBTR-H D Indian Council of Agricultural Research Ministry of Science and Technology Central Marine Fisheries Research Institute Department of Biotechnology CMFRI Training Manual Series No.15/2018 Training Manual In the frame work of the project: DBT sponsored Three Months National Training in Molecular Biology and Biotechnology for Fisheries Professionals 2015-18 Training Manual In the frame work of the project: DBT sponsored Three Months National Training in Molecular Biology and Biotechnology for Fisheries Professionals 2015-18 Training Manual This is a limited edition of the CMFRI Training Manual provided to participants of the “DBT sponsored Three Months National Training in Molecular Biology and Biotechnology for Fisheries Professionals” organized by the Marine Biotechnology Division of Central Marine Fisheries Research Institute (CMFRI), from 2nd February 2015 - 31st March 2018. Principal Investigator Dr. P. Vijayagopal Compiled & Edited by Dr. P. Vijayagopal Dr. Reynold Peter Assisted by Aditya Prabhakar Swetha Dhamodharan P V ISBN 978-93-82263-24-1 CMFRI Training Manual Series No.15/2018 Published by Dr A Gopalakrishnan Director, Central Marine Fisheries Research Institute (ICAR-CMFRI) Central Marine Fisheries Research Institute PB.No:1603, Ernakulam North P.O, Kochi-682018, India. 2 Foreword Central Marine Fisheries Research Institute (CMFRI), Kochi along with CIFE, Mumbai and CIFA, Bhubaneswar within the Indian Council of Agricultural Research (ICAR) and Department of Biotechnology of Government of India organized a series of training programs entitled “DBT sponsored Three Months National Training in Molecular Biology and Biotechnology for Fisheries Professionals”. -
Evaluación Del Crecimiento Y Supervivencia Del
TECNOLÓGICO NACIONAL DE MÉXICO Instituto Tecnológico de Boca del Río Dirección de Promoción Cultural y Deportiva “Año del Centenario de la Promulgación de la Constitución Política de los Estados Unidos Mexicanos” SECRETARÍA DE EDUCACIÓN PÚBLICA DIRECCIÓN GENERAL DE EDUCACIÓN SUPERIOR TECNOLÓGICA INSTITUTO TECNOLÓGICO DE BOCA DEL RÍO DIVISIÓN DE ESTUDIOS DE POSGRADO E INVESTIGACIÓN EFECTO DEL COLOR DE TANQUE Y DE DOS DIETAS ENRIQUECIDAS, SOBRE LA SUPERVIVENCIA Y DESARROLLO DE LARVAS DEL FALSO PEZ PAYASO Amphiprion ocellaris CUVIER, 1830. TESIS QUE COMO REQUISITO PARA OBTENER EL GRADO DE MAESTRO EN CIENCIAS EN ACUACULTURA PRESENTA ING. DANIEL SERRANO ARROYO DIRECTOR DE TESIS DR. CARLOS IVÁN PÉREZ ROSTRO OCTUBRE 2017 BOCA DEL RÍO, VERACRUZ, MÉXICO Km. 12 Carr. Veracruz-Córdoba, Boca del Río, Ver. C.P. 94290 Tel. (01 229) 6905010 e-mail: [email protected] | www.itboca.edu.mx ACTA DE REVISIÓN DE TESIS AUTORIZACIÓN DE IMPRESIÓN DE TESIS 1. RESUMEN Durante la presente investigación, se evaluó el efecto de tres colores de tanque y dos dietas enriquecidas, sobre el desarrollo y supervivencia de larvas del falso pez payaso Amphiprion ocellaris a partir de un diseño bifactorial de 3x2 con 4 réplicas por tratamiento. Las coloraciones de las unidades experimentales fueron: A) Azul, N) Negro, B) Blanco y trasparente como control (C), y las dietas evaluadas fueron: S: presa viva enriquecida con Selco y M: presa viva enriquecida con Microalga (Nannochloropsis sp.). Se usó una densidad de siembra de 4 larvas/l. Durante los primeros 25 días de vida se evaluó la supervivencia de las larvas y el tiempo (días) que tardó cada larva en formar la banda blanca característica de la especie, la cual representa el final de la fase larvaria e inicio de la fase juvenil. -
An Assemblage of the Host Anemone Heteractis Magnifica in the Northern
J. Mar. Biol. Ass. U.K. (2004), 84,671^674 Printed in the United Kingdom An assemblage of the host anemone Heteractis magni¢ca in the northern Red Sea, and distribution of the resident anemone¢sh Ð O P Thea Marie Brolund* , Anders Tychsen , Lis Engdahl Nielsen* and Michael Arvedlund O *The August Krogh Institute, University of Copenhagen, Universitetsparken 13, DK-2100 Copenhagen Ò, Denmark. Geological P Museum, University of Copenhagen, ster Voldgade 5^7, DK-1350 Copenhagen K, Denmark. ÐSesoko Station, Tropical Biosphere Research Center, University of the Ryukyus, 3422 Sesoko, Motobu, Okinawa 905-0227, Japan. Present address of corresponding author: Department of Marine Biology, James Cook University,Townsville QLD 4811, Australia. E-mail: [email protected] The Heteractis magni¢ca assemblage at the tip of the Sinai Peninsula was examined. The actinian size, location, and number of resident anemone¢shes were recorded. The anemones were found at depths down to approximately 40 m and the sizes of clustering H. magni¢ca and clusters were positively correlated with depth. The shallow waters of the anemone assemblage contained few mainly small, solitary actinians. There seemed to be a tendency for solitary actinians to cluster once they reached a certain size-range. The resident anemone¢shes Amphiprion bicinctus and Dascyllus trimaculatus were present in very large numbers (approximately 250 and 1800 respectively) and the A. bicinctus home range size was positively correlated with depth. INTRODUCTION The aim of this study was a mapping and descriptive analysis of the Ras Mohammed H. magni¢ca assemblage Ten species of tropical giant sea anemones (Families: and its resident A. -
Universidade Federal Do Ceará Centro De Ciências Agrárias Departamento De Engenharia De Pesca Programa De Pós-Graduação Em Engenharia De Pesca
0 UNIVERSIDADE FEDERAL DO CEARÁ CENTRO DE CIÊNCIAS AGRÁRIAS DEPARTAMENTO DE ENGENHARIA DE PESCA PROGRAMA DE PÓS-GRADUAÇÃO EM ENGENHARIA DE PESCA CARLOS HENRIQUE PROFIRIO MARQUES CARACTERIZAÇÃO DO AQUARISMO MARINHO NO ESTADO DO CEARÁ FORTALEZA 2020 1 CARLOS HENRIQUE PROFIRIO MARQUES CARACTERIZAÇÃO DO AQUARISMO MARINHO NO ESTADO DO CEARÁ Tese apresentada à Coordenação do Programa de Pós-Graduação em Engenharia de Pesca da Universidade Federal do Ceará, como parte dos requisitos para obtenção do título de Doutor em Engenharia de Pesca. Área de concentração: Aquicultura. Orientador: Prof. Dr. Francisco Hiran Farias Costa. FORTALEZA 2020 Dados Internacionais de Catalogação na Publicação Universidade Federal do Ceará Biblioteca Universitária Gerada automaticamente pelo módulo Catalog, mediante os dados fornecidos pelo(a) autor(a) M316c Marques, Carlos Henrique Profirio. Caracterização do aquarismo marinho no estado do Ceará / Carlos Henrique Profirio Marques. – 2020. 81 f. : il. color. Tese (doutorado) – Universidade Federal do Ceará, Centro de Ciências Agrárias, Programa de Pós- Graduação em Engenharia de Pesca, Fortaleza, 2020. Orientação: Prof. Dr. Francisco Hiran Farias Costa. 1. Aquário. 2. Corais de recifes. 3. Peixes ornamentais. I. Título. CDD 639.2 2 CARLOS HENRIQUE PROFIRIO MARQUES CARACTERIZAÇÃO DO AQUARISMO MARINHO NO ESTADO DO CEARÁ Tese apresentada à Coordenação do Programa de Pós-Graduação em Engenharia de Pesca da Universidade Federal do Ceará, como parte dos requisitos para obtenção do título de Doutor em Engenharia de Pesca. Área de concentração: Aquicultura. Aprovada em: ______ / ______ / ___________ BANCA EXAMINADORA _________________________________________________ Prof. Dr. Francisco Hiran Farias Costa (Orientador) Universidade Federal do Ceará (UFC) _________________________________________________ Prof. Dr. José Renato de Oliveira César Universidade Federal do Ceará (UFC) _________________________________________________ Prof. -
Report on the Survey of the Marine Aquarium Fishery Batticoloa and Ampara Districts, Sri Lanka
Report on the survey of the Marine Aquarium Fishery Batticoloa and Ampara Districts, Sri Lanka. 2008 1. Introduction The marine aquarium fishery in the eastern coastal waters has been in existence since the beginning of the industry in Sri Lanka. The present value of the marine ornamental sector of the aquarium fish industry is believed to be about 60% of the total value of about US $ 7 million. Marine aquarium species of the eastern coastal reefs is vital for the industry. A number of key species of butterflyfish (Chaetodontidae), angelfish (Pomacanthidae), wrasses (Labridae), gobies (Gobiidae), damselfish (Pomacentridae), groupers (Serranidae), blennies (Blennidae), surgeonfish (Acanthuridae) and invertebrates such as the scarlet shrimps (Lysmata debelius) and painted shrimps (L. amboinensis) are harvested from the eastern coastal reefs. Prior to late 1980’s the collecting areas were widespread in Trincomalee and Batticoloa Districts. Since mid 1990’s the collecting areas diminished due to restrictions placed by the defense authorities as a result of the internal conflict that prevailed at the time. The fishery is conducted during the calm season from March to October and divers, also called collectors from the southern and western coastal areas migrate to the east to join local divers from the east coast. Aquarium species are collected by snorkeling in shallow inshore reefs and by scuba diving in offshore reefs to a depth of about 35m. About 250 species of reef fish and about 50 species of invertebrates are collected for export. The earliest comprehensive study of the marine aquarium fish industry in Sri Lanka was carried out by Wood (1985). -
Ontogenetic and Phylogenetic Simplification During
Salis et al. BMC Biology (2018) 16:90 https://doi.org/10.1186/s12915-018-0559-7 RESEARCH ARTICLE Open Access Ontogenetic and phylogenetic simplification during white stripe evolution in clownfishes Pauline Salis1, Natacha Roux1, Olivier Soulat2, David Lecchini3, Vincent Laudet1* and Bruno Frédérich4 Abstract Background: Biologists have long been fascinated by the striking diversity of complex color patterns in tropical reef fishes. However, the origins and evolution of this diversity are still poorly understood. Disentangling the evolution of simple color patterns offers the opportunity to dissect both ultimate and proximate causes underlying color diversity. Results: Here, we study clownfishes, a tribe of 30 species within the Pomacentridae that displays a relatively simple color pattern made of zero to three vertical white stripes on a dark body background. Mapping the number of white stripes on the evolutionary tree of clownfishes reveals that their color pattern diversification results from successive caudal to rostral losses of stripes. Moreover, we demonstrate that stripes always appear with a rostral to caudal stereotyped sequence during larval to juvenile transition. Drug treatments (TAE 684) during this period leads to a dose-dependent loss of stripes, demonstrating that white stripes are made of iridophores and that these cells initiate the stripe formation. Surprisingly, juveniles of several species (e.g., Amphiprion frenatus) have supplementary stripes when compared to their respective adults. These stripes disappear caudo-rostrally during the juvenile phase leading to the definitive color pattern. Remarkably, the reduction of stripe number over ontogeny matches the sequences of stripe losses during evolution, showing that color pattern diversification among clownfish lineages results from changes in developmental processes. -
Hatchery Production of the Clownfish Amphiprion Nigripes at Agatti Island, Lakshadweep, India
623 © 2012 Triveni Enterprises J. Environ. Biol. Vikas Nagar, Lucknow, INDIA 33, 623-628 (2012) [email protected] ISSN: 0254-8704 Full paper available on: www.jeb.co.in CODEN: JEBIDP Hatchery production of the clownfish Amphiprion nigripes at Agatti island, Lakshadweep, India Author Details T.T. Ajith Kumar Centre for Marine Living Resources and Ecology - Field Research Station, Agatti Island - 682 553, Lakshadweep, India (Corresponding author ) e-mail : [email protected] M. Gopi Centre for Marine Living Resources and Ecology - Field Research Station, Agatti Island - 682 553, Lakshadweep, India K.V. Dhaneesh Centre for Marine Living Resources and Ecology - Field Research Station, Agatti Island - 682 553, Lakshadweep, India R. Vinoth Centre for Marine Living Resources and Ecology - Field Research Station, Agatti Island - 682 553, Lakshadweep, India S. Ghosh Centre for Marine Living Resources and Ecology - Field Research Station, Agatti Island - 682 553, Lakshadweep, India T. Balasubramanian Centre for Marine Living Resources and Ecology - Field Research Station, Agatti Island - 682 553, Lakshadweep, India T. Shunmugaraj Centre for Marine Living Resources and Ecology - Field Research Station, Agatti Island - 682 553, Lakshadweep, India Abstract Healthy individuals of matured clownfish, Amphiprion nigripes and sea anemone, Heteractis magnifica were Publication Data collected from the Agatti island lagoon by snorkeling. During ‘conditioning’ for 3 months, pair formation occurred and the same were transferred to rectangular fiber glass spawning tanks of 1000 l capacity. Suitable water Paper received: quality parameters were maintained. The fishes were fed with tuna eggs, boiled clam meat, squid, octopus and 11 November 2010 trash fish thrice in a day. Reproductive behaviour and embryonic development were documented. -
Hermaphroditism in Fish
Tesis doctoral Evolutionary transitions, environmental correlates and life-history traits associated with the distribution of the different forms of hermaphroditism in fish Susanna Pla Quirante Tesi presentada per a optar al títol de Doctor per la Universitat Autònoma de Barcelona, programa de doctorat en Aqüicultura, del Departament de Biologia Animal, de Biologia Vegetal i Ecologia. Director: Tutor: Dr. Francesc Piferrer Circuns Dr. Lluís Tort Bardolet Departament de Recursos Marins Renovables Departament de Biologia Cel·lular, Institut de Ciències del Mar Fisiologia i Immunologia Consell Superior d’Investigacions Científiques Universitat Autònoma de Barcelona La doctoranda: Susanna Pla Quirante Barcelona, Setembre de 2019 To my mother Agraïments / Acknowledgements / Agradecimientos Vull agrair a totes aquelles persones que han aportat els seus coneixements i dedicació a fer possible aquesta tesi, tant a nivell professional com personal. Per començar, vull agrair al meu director de tesi, el Dr. Francesc Piferrer, per haver-me donat aquesta oportunitat i per haver confiat en mi des del principi. Sempre admiraré i recordaré el teu entusiasme en la ciència i de la contínua formació rebuda, tant a nivell científic com personal. Des del primer dia, a través dels teus consells i coneixements, he experimentat un continu aprenentatge que sens dubte ha derivat a una gran evolució personal. Principalment he après a identificar les meves capacitats i les meves limitacions, i a ser resolutiva davant de qualsevol adversitat. Per tant, el meu més sincer agraïment, que mai oblidaré. During the thesis, I was able to meet incredible people from the scientific world. During my stay at the University of Manchester, where I learned the techniques of phylogenetic analysis, I had one of the best professional experiences with Dr. -
Clownfish : Comercial Interest and Culture
UNIVERSIDAD DE LAS PALMAS DE GRAN CANARIA María Kristel Ortega García Tutores: Grado en Ciencias del Mar María Ascensión Viera Rodríguez Curso 2013/2014 Lucía Molina Domínguez Clownfish: commercial interest and culture Nombre: María Kristel Apellidos: Ortega García Titulación que cursa: Grado Ciencias del Mar Curso académico: 2013‐2014 Tutor: María Ascensión Viera Rodríguez Departamento de Biología de la Universidad de Las Palmas de Gran Canaria Cotutor: Lucía Molina Domínguez Investigadora asociada al IUSA María Kristel Ortega García María Ascensión Viera Rodríguez Lucía Molina Domínguez María Kristel Ortega García Index 1. Introduction Page 2 1.1 History of the aquarium Page 2 1.2 Ornamental marine species of commercial importance Page 3 1.3 Importance of Pomacentridae family Page 4 1.3.1 International trade of marine ornamental species Page 5 1.3.2 Major exporters and importers countries Page 6 1.3.3 Wild caught animals and the impact on the ecosystem Page 7 1.4 Family and genus characteristics Page 8 1.4.1 Taxonomy Page 8 1.4.2 Biology Page 9 1.4.3 Distribution Page 9 2. Culture of clownfish Page 10 2.1 Sex change Page 10 2.2 Spawning and hatching Page 11 2.3 Egg development Page 13 2.4 Feeding habits Page 15 2.5 Experimental work Page 16 2.5.1 Installation Page 17 2.5.2 Maintenance Page 17 2.5.3 Parameters Page 18 3. Conclusions Page 19 4. Bibliography Page 21 1 María Kristel Ortega García 1. Introduction The term “ornamental fish " is a generic term describing to those aquatic organisms supported in an aquarium with intention of ornament, including fish, invertebrate as corals, crustaceans, mollusks, echinoderms, as well as alive rock (Panné & Luchini, 2008). -
Zoology Publications 1 Fieldiana: Zoology Pub
Zoology Publications 1 Fieldiana: Zoology Pub. No. Volume 1 (Complete in Eighteen Numbers) 5 No. 1. On the Structure and Development of the Vertebral Column of Amia. By O.P. Hay. 1895. 54 pages, 3 color illus. 7 No. 2. On Certain Portions of the Skeleton of Protostega gigas. By O.P. Hay. 1895. 8 pages, 2 illus. 11 No. 3. On Sundry Collections of Mammals Received by the Field Columbian Museum from Different Localities, with Descriptions of Supposed New Species and Sub-Species. By D. G. Elliot. 1896. 18 pages, 10 illus. 12-13 Nos. 4 and 5. On Some Collections of Fishes Made in the Kankakee and Illinois Rivers. By O.P. Hay. 1896. 16 pages. On the Skeleton of Toxochelys latiremus. By O.P. Hay. 1896. 8 pages, 2 illus. 19-20 Nos. 6 and 7. List of Mammals from Somali-Land Obtained by the Museum’s East African Expedition. By D.G. Elliot. 1897. 49 pages. Remarks upon Two Species of Deer of the Genus Cervus from the Philippine Archipelago. By D.G. Elliot. 1897. 2 pages, 24 illus. 22 No. 8. List of Fishes and Reptiles Obtained by Field Columbian Museum East African Expedition to Somali-Land in 1896. By S.E. Meek. 1897. 24 pages, 2 illus. 26 No. 9. List of Collection of Shells from the Gulf of Aden, Obtained by the Museum’s East African Expedition. By W.H. Dall. 1898. 6 pages. 27 No. 10. List of Species of Mammals, Principally Rodents, Obtained by W.W. Price, Dr. S.E. Meek, G.K. -
Larval Development and Growth of Red Saddleback Anemonefish, Amphiprion Ephippium,(Bloch, 1790) Under Captive Conditions
Indian Journal of Geo Marine Sciences Vol. 47 (12), December 2018, pp. 2421-2428 Larval development and growth of Red Saddleback Anemonefish, Amphiprion ephippium,(Bloch, 1790) under captive conditions Rohini Krishna, M. V 1*, Anil M. K2 & Neethu Raj. P3 1 Department of Zoology,TKM College of Arts and Science, Kollam - 691 005, Kerala, India 2,3 Vizhinjam Research Centre of ICAR-Central Marine Fisheries Research Institute, Vizhinjam,Thiruvananthapuram-695521,Kerala, India * [E.mail: [email protected]] Received 01 May 2017; revised 24 July 2017 On the 1stday of hatching, the body of the larva was transparent and all the fins were fused together to form a single fin fold. Hatchlings measured 4.96mm in total length. On the 10thday, all the fins were visible and body colouration had begun to develop, the larvae then measured 7.08 mm in total length. The banding began to appear from the 10th day and on the 15thday, the head and middle band were clearly visible. From the 25th day onwards, the larva measured 9.66 mm in total length. On the 30th day, adult pigmentation had begun to appear in the larva. After the 45thday, the bands started to disappear. By the 160thday, the middle band had completely disappeared. On the 310th day all the bands had disappeared and now the juvenile has transformed into an adult fish. [Keywords: Amphiprion ephippium, Chromatophores, Pigmentation, Clownfish] Introduction fish is widely distributed throughout Eastern Indian The marine ornamental trade industry has been Ocean and has been known to associate with 3 flourishing in the recent years even though there was different sea anemones. -
Pomacan 1983 Fao Species Identification Sheets
click for previous page POMACAN 1983 FAO SPECIES IDENTIFICATION SHEETS FISHING AREA 51 (W. Indian Ocean) POMACANTHIDAE Angelfishes Body elongate-oval to orbicular in shape, strongly compressed. Mouth very small, terminal, protrusible, the gape not extending to anterior rim of orbit; snout never produced; teeth setiform, usually arranged in brush-like bands in jaws; preoercle always with a strong spine at angle. Dorsal fin with 9 to 15 spines and 15 to 33 soft rays, continuous (never notched and sometimes, along with anal fin, greatly extended into filaments; anal fin with 3 spines and 14 to 25 soft rays. Scales ctenoid (rough to touch), ribbed, small to moderate in size, rounded to angular in shape, extending onto soft portions of vertical fins; no axillary scaly process at bases of pelvic fins; lateral line complete or nearly so. Colour: brightly coloured fishes; ground colour white, grey, yellow, orange, red, blue, black or brown; often marked with bars, stripes, or spots of white, yellow, orange, blue, black or brown. Juveniles often differently coloured than adults. continuous dorsal fin filamentous rays sometimes present mouth small, terminal, protrusible preopercular spine no scaly process filamentous rays sometimes present - 2 - FAO Sheets POMACANTHIDAE Fishing Area 51 Angelfishes inhabit shallow coral reef areas in depths above 30 to 40 m. A few species range to depths of 80 m or more. As adults they feed mainly on sponges, while juveniles eat principally algae. Species of the genus Pomacanthus grow to a relatively large size (50 to 60 cm) and are frequently seen in markets, although they are considered commercially unimportant.