“A BARREL OF : A TESTUDINARIA COMPLEX IN THE VERDE ISLAND PASSAGE, PHILIPPINES”

A Thesis submitted to the faculty of A* San Francisco State University In partial fulfillment of the requirements for ‘fylOlr the Degree

Master of Science

In

Biology: Ecology, Evolution, and Conservation Biology

by

Joseph Elliz B. Comendador

San Francisco, California

January 2020 Copyright by Joseph Elliz B. Comendador 2020 CERTIFICATION OF APPROVAL

I certify that I have read A BARREL OF SPONGES: A XESTOSPONGIA

TESTUDINARIA SPECIES COMPLEX IN THE VERDE ISLAND PASSAGE,

PHILIPPINES by Joseph Elliz B. Comendador, and that in my opinion this work meets the criteria for approving a thesis submitted in partial fulfillment of the requirement for the degree Master of Science in Biology: Ecology Evolution and Conservation at San

Francisco State University.

Terrence M. Gosliner, Ph.D. Research Professor

Rich Mooi, Ph.D. Research Professor

Sarah Cohen, Ph.D. Professor A BARREL OF SPONGES: A XESTOSPONGIA TESTUDINARIA SPECIES COMPLEX IN THE VERDE ISLAND PASSAGE, PHILIPPINES

Joseph Elliz B. Comendador San Francisco, California 2020

The giant barrel Xestospongia testudinaria is a dominant member of the coral reef community in the Verde Island Passage (VIP) in the Philippines. However, little is known about its morphology and gene flow. Barrel sponge morphotypes, osculum diameter, body circumference, body length, spicule length, and spicule width were all recorded, analyzed and combined with mitochondrial COI gene results to establish a possible pattern useful to understand or refute the morphological concept of barrel sponge classification. ABGD analysis revealed four haplotypes for the VIP. Spicule variation showed no significant differences and no correlation among sites and haplotypes. On the other hand, colony dimensions showed no significant difference and no correlation among sites and haplotypes. Colony form showed significant variation within and among sites and significant differences linked with haplotypes. My research findings agree with global studies on barrel sponges, showing the vast majority of Xestospongia (including X. muta from the ) are of the same haplotype. The lack of variation between the common haplotype of X. testudinaria and X. muta suggests that X muta from the Caribbean is the same species as X testudinaria. Interestingly, the Coral Triangle region of the Indo-Pacific showed greater variation in haplotypes, especially within Indonesia and the Philippines than between these areas of the Coral Triangle and the rest of the world.

I certify that the Abstract is a correct representation of the content of this thesis.

October 28,2019

Date

iv ACKNOWLEDGEMENTS

I would like to thank first and foremost the Almighty God, for giving me this opportunity and for keeping me safe throughout my two years of stay in San Francisco, California. I extend my thanks to my thesis committee, Dr. Rich Mooi, Dr. Sarah Cohen, Dr. Dirk

Erpenbeck (LMU-Germany) and especially to Dr. Terrence Gosliner, for all the time, patience and guidance that they have provided throughout this project. I wish also to thank my dive guide, PJ Aristorenas, and his wife Jaichelle, who helped during fieldwork. Also,

I would like to thank the Center for Comparative Genomics, especially Dr. Athena Lam and Boni Cruz for assistance in analyzing the molecular data, troubleshooting during sequencing and for patiently guiding me. I would like to extend my deepest gratitude to the

CAS wet laboratory team, headed by Christina Piotrowski, with Elizabeth Kools and

Johanna Loacker, for providing me lab space, chemicals, and compound microscope.

Special mention goes to Dr. Gary Williams for help in the SEM laboratory. I thank my

Gosliner/Mooi lab mates: Lynn Bonomo, Alexis Coles, Lisa Paggeot, Kelly Larkin, Kelly

Markello, Francis Armstrong, Emily Otstott and especially to Dmitri Smirnoff and Sam

Donohoo for their ideas, expertise, and guidance. The management of the National

Museum of the Philippines allowed me to pursue my degree abroad. The endless support of my family, especially of my dear wife Jam, is forever appreciated. Lastly, this research would never have been possible without the support of the Lakeside Foundation, which

funded my entire graduate school program at San Francisco State University and California

Academy of Sciences. Thank you very much.

v TABLE OF CONTENTS

List of Tables...... vii

List of Figures ...... viii

List of Appendices...... x

Introduction...... 1

Impact Statement...... 5 Hypothesis...... 6

Methods...... 7

Rationale...... 7 Collection and Examination of Barrel Sponges in the Field...... 7 Identification of Sponge Morphological Types...... 11 Slide Preparation, Spicule Measurement and Scanning Electron Microscopy 12 Molecular Work: DNA Extraction, PCR, Gel Electrophoresis, PCR Purification, ExoSAP-IT, Cycle Sequencing and Ethanol Precipitation...... 15 Preparing Sequence Reactions for the ABI Prism 3130...... 19 Mitochondrial Data Analysis and Interpretation...... 19 Results...... 20

A. Morphological Types ...... 20 B. Spicule Measurements...... 25 C. Mitochondrial DNA Analysis...... 32

Discussions...... 40 References...... 47

Appendices...... 52 LIST OF TABLES

Table Page

1. Recipe sample for a 25 p.L PCR reaction...... 18

2. One Way ANOVA analysis of mean body height for each dive site...... 22

3. One Way ANOVA analysis of mean sponge osculum diameter per dive site . 22

4. One Way ANOVA analysis of mean sponge body circumference per dive site . 22

5. One Way ANOVA analysis of mean spicule length and width per dive site . 25

6. Tukey’ s post hoc test (spicule length)...... 26

7. Tukey’s post hoc test (spicule width)...... 26

8. One Way ANOVA ofX. testudinaria spicule length and width from El Pinoy 28

9. Tukey test of X. testudinaria spicule length and width from El Pinoy...... 39

10. One Way ANOVA ofXestospongia testudinaria spicule length and width from Dead

Palm...... 30

11. Tukey test of X. testudinaria spicule length and width from Dead Palm...... 31

12. Haplotypes of X. testudinaria from the Verde Island Passage Philippines 32

13. Nucleotide difference for mitochondrial marker COI...... 43

14. VIP Haplotypes in relation to morphotypes, spicule length, and spicule width 44 LIST OF FIGURES Figures Page

1. An example of a bleached and normal barrel sponge...... 5

2. Field collection sites in Mabini-Tingloy and Isla Verde in Batangas Province 9

3. Sponge fragments being taken using an apple corer and ecological parameters being recorded...... 9

4. Sponge body circumference (left) and osculum diameter ( ght) being measured and recorded...... 10

5. Sponge sample taken with an apple corer...... 10

6. Morphological types of Xestospongia testudinaria in the Verde Island Passage...... 11

7. Spicule length measured with a calibrated eyepiece graticule...... 13

8. An SEM image of oxea spicules...... 14

9. Distribution of morphological types at each dive site...... 21

10. Map of morphotype distribution...... 21

11. Mean sponge body circumference (cm) per dive site...... 23

12. Mean sponge body height (cm) per dive site...... 24

13. Mean sponge osculum diameter (cm) per dive site...... 24

14. Mean sponge spicule length (urn) per dive site...... 27

15. Mean sponge spicule width (urn) per dive site...... 27

16. SEM image of oxea spicule variation found in Xestospongia testudinaria individuals in the VIP...... 28

17. Images of the four haplotypes of Xestospongia testudinaria found in the VIP.... 35 18. Phylogenetic rooted tree based on n tochondrial COI DNA of Xestospongia testudinaria from the Verde Island Passage with its corresponding morphotypes ...... 36

19. Magnified portion of phylogenetic rooted tree based on mitochondrial COI DNA of Xestospongia testudinaria with corresponc ng morphotypes and haplotypes...... 37

20. Phylogenetic tree based on mitochondrial COI DNA of X. testudinaria from the Philippines, including Caribbean, Myanmar, Viet Nam and the Red Sea 38

21. Phylogenetic haplotype tree representation...... 39

22. Schematic view of the COI standard barcoding region (Folmer et al. 1994). and the primer used by Swierts et al. (2013)...... 42 APPENDICES

Appendix Page

1. P-distance values of X. testudinaria in the VIP...... 51

x INTRODUCTION

Our planet is 70% ocean, and we have not come close to fully exploring the scope of marine biodiversity. The Indo-Pacific is the world’s largest marine biogeographic region, from tropical and subtropical waters from the Red Sea in the Western Indian Ocean to Easter Island in the Pacific (Erpenbeck et al., 2017). It is recognized as a biodiversity hotspot for marine taxa including sponges (Briggs, 1999; Hoeksema, 2007; Bell wood &

Meyer, 2009; Hooper and Levi, 1994; Van Soest, 1994). According to Veron (1995),

Hoeksema (2007) and Swierts et al. (2013), the genetic diversity of the marine biota in this region remains incomoletely studied. Sponges are an abundant and diverse group of marine organisms in this region and their genetic connectivity, systematics and biogeography are poorly understood. Obtaining data to understand genetic diversity is pivotal to the assessment of gene flow among and within populations and species (Setiawan et al., 2015).

The Philippines is at the apex of the “Coral Triangle” that includes Indonesia, Papua New

Guinea, Timor Leste plus the Solomon Islands. Carpenter and Springer (2005) stated that the Coral Triangle is an “ecological hotspot” and a center of marine biodiversity, within which there is higher concentration of species per unit area in the Philippines than anywhere in Indonesia, making it an important region for conservation and economic management. The broad distribution of many marine organisms, including sponges, has been attributed to the lumping of morphologically similar but often evolutionary distinct lineages into single species. Molecular studies have indicated the presence of multiple genetically differentiated lineages within morphologically identical samples, i.e. cryptic species according to the findings of Knowlton (2000). Marine sponges are diverse and structurally important components of coral reefs (Swierts et al., 2013). According to

1 Bell (2008), despite its pivotal role for the functionality of aquatic ecosystems, a trustworthy estimation of poriferan species richness in the Indo-Pacific appears difficult

(Hooper and Levi, 1994; Hooper et al., 1999). In terms of species richness, abundance, and biomass, sponges rival both hard and soft corals. Also, sponges provide refuge for small marine invertebrates, are critical to benthic-pelagic coupling and have symbiotic associations with microorganisms (Diaz and Rutzler, 2001). Some species such as the galatheid crab siagiani, appear to be obligate symbionts of Xestospongia.

Microbial associates occur in varied symbiotic relationships (Diaz and Rutzler, 2001).

Though the ecological importance of marine sponges is well established, molecular studies on sponges in the Indo-Pacific are scarce, particularly in the Coral Triangle. No detailed studies have been performed in the Verde Island Passage (VIP) of the Philippines, even though it is demonstrably the most diverse of all water bodies within the Coral Triangle

(Lopez-Legentil and Pawlik, 2008; Worheide, 2006; Bentlage and Worheide, 2007;

Worheide et al., 2008).

Xestospongia (Demospongiae: : ' contains some of the largest known marine sponges, attaining sizes of 2.4 m in both height and width (Swierts et al., 2013) and composing up to 9% of the area of some reefs (Zea, 1993). Barrel sponges are found in the Indo-Pacific, Red Sea, East Africa and Great Barrier Reef in Australia,

Tonga, and the tropical Atlantic Ocean. Larger species of Xestospongia have an erect, volcano- or barrel-shaped structure with individuals varying in size, shape, and composition. The external morphology of giant barrel sponges can exhibit smooth to highly digitate or lamellate surfaces (Setiawan, 2015). According to Kerr and Borges (1994), the

2 color of the sponge in life is caramel brown to maroon brown externally, with a beige interior. The maroon coloration is sometimes patchy and can be due to the presence of symbionts (genus: Synechococcus) in the portions of the sponge surface exposed to sunlight. Unlike cells of other , sponge cells do not form various organs

such as kidney, liver, or nerves. Sponges are efficient filter-feeders of the ocean, filtering -size food particles then expelling the filtered water through their osculum

(McMurray et al., 2010). An individual barrel sponge can filter up to 50,000 times its own volume of water every day (Pawlik, 2008). Sponges themselves become food for other marine animals such as sea turtles, fish, echinoderms and nudibranchs. Sponges primarily defend themselves from such predation with chemical defenses, which include secondary metabolites (Angermeier, et al., 2010; Bertin and Callahan, 2008; Jones et al., 2005;

McMurray et al., 2010). Sponges can reproduce in three ways: sexual, hermaphroditic, and asexual (the latter exhibited if the external conditions are favorable). Sponge larvae can disperse over short or long distances, depending on the species (Uriz, et al., 1998; Mariani et al., 2000; Maldonado and Uriz, 1999: Vacelet, 1999; Lazoski et al., 2001). According to

Pawlik et al. (2009), sponge gene flow is often correlated with larval dispersal capabilities and population structure, yet the existing molecular studies have focused only on the most problematic taxonomic groups (Knowlton, 2000).

Xestospongia have very long life spans (possibly over 2000 years). X muta has been called the “redwood of the reef’ due to its size, longevity and ecological importance

(McMurray et al., 2008). The majority of sponge studies have focused on the distribution and evolution of marine species at small spatial scales. According to Cowman and

3 Bell wood (2013), these analyses can become more useful when they are integrated with more wide-ranging studies. Swierts et al. (2013) stated that due to the almost ubiquitous presence of barrel sponges in coral reefs, it is a good model species for detailed studies of sponge spatial patterns of morphological and genetic variation. The research conducted by

Swierts et al. (2013) in Indonesia discovered that there were four morphotypes (digitate, lamellate, smooth, and intermed: ate) o f Xestospongia testudinaria around Lembeh Island.

They noticed that the digitate morphology dominated in the waters surrounding Bitung port and other human settlements, relative to other morphotypes. They also found significant differences in genetic composition among these morphotypes, suggesting these are not mere ecophenotypic variations, but rather reproductively isolated units (Swierts et al.,

2013). My research focused on the barrel sponge Xestospongia testudinaria found in the

Philippines, in the Verde Island Passage between the islands of Luzon and Mindoro. The

VIP is located within the globally significant Coral Triangle, an area considered to exhibit the highest levels of the world’s marine biodiversity. The VIP spans five provinces:

Batangas, Occidental Mindoro, Oriental Mindoro, Marinduque, and Romblon. The abundant fish and charismatic megafauna in this region support over 7 million people, whose livelihoods include fishing, aquaculture, and tourism. In this study, I was able to record morphological data from 95 sponge individuals, sampling their tissues for molecular analysis. A molecular phylogenetic tree was generated to determine relationships among the measured morphological and ecological parameters and genetic sequence.

4 IMPACT STATEMENT

Members of the genus Xestospongia are known to provide habitat for countless fish

species and marine invertebrates (i.e. shrimp, worms, etc.) and some spccies such as the

galatheid crab Lauriea siagiani, appear to be obligate symbionts of Xestospongia.

Microbial associates occur in varied symbiotic relationships (Diaz and Rutzler, 2001).

Also, sponges are used in biomedical research and are a key source of pharmaceutical

compounds {Lopez-Legentil and Pawlik, 2008). The sustainability of the coral reef

ecosystem s also reliant on sponges. Recent research i ndicates that bleaching is not limited to reef-building corals, as it can affect all reef species with photosynthetic symbionts,

including sponges (Fig. 1 and McMurray et al., 2011). Therefore, it is crucial to gather data that describe the biodiversity and genetic structure of populations of Xestospongia.

Figure 1. An example of a bleached (left) and normal (right) barrel sponge photographed August 5, 2018 in Tingloy, Batangas. Philippines.

There are approximately 300 sponge researchers worldwide. However, in the

Philippines, there are only two researchers known to focus on sponge

(Schonberg, 2015). According to Setiawan (2015), given the general lack of biodiversity

5 research in crucial portions of the Coral Triangle, it should come as no surprise that sponge

research in southeast Asia, including the Philippines, is almost completely lacking.

My research is intended to test some of the most significant findings of a similar

study conducted by Swierts et al. (2013) in Indonesia on Indo-Pacific X. testudinaria.

Because that study was geographically constrained, it is important to expand their pioneering work geographically to test the generality of their results, and to discover if they

apply to the waters around the most diverse coral reefs on Earth.

HYPOTHESES

To determine if distinct morphotypes of Xestospongia can be identified, and if these morphotypes can be correlated with genetic differences:

AIM 1: Test for morphological diversity within X. testudinaria in the VIP that could

support the existence of a species complex.

AIM 2: Test for differences in mitochondrial genetic composition among morphotypes

o fX testudinaria.

6 METHODS

AIM 1:

Test for morphological diversity within X. testudinaria in the VIP that could support the existence of a species complex.

Rationale:

Literature and records on morphological diversity of Indo-Pacific X testudinaria are lacking, particularly in the Philippines. Analysis of different morphological characters exhibited by X. testudinaria will allow assessment of whether previously established taxonomic techniques can be used to assess the likelihood of a species complex in the

Verde Island Passage. I explored whether it is possible to characterize a complex using these techniques.

Methodology:

Collection and Examination of Barrel Sponges in the Field:

During the monsoon months of June-August 2018, I travelled to the municipalities of

Mabini, Verde Island and Tingloy in Batangas, Philippines to sample sponges from the

VIP, with a total of six days in the field and two dives per day. Ten SCUBA dive sites (Fig.

2) were identified from established tourist dive spots at which the presence of X. testudinaria has been reported by local dive guides: Pinnacle, San Agapito, El Pinoy,

7 Mainit Bubbles, Dead Palm, Devils Point, Sombrero, Beatrice, Bethlehem, and Cathedral.

The different morphological types were observed, recorded, photographed and sampled.

Special collection permits from the provincial and municipal government of Batangas and

Bureau of Fisheries and Aquatic Resources (BFAR) were secured months before field collection. Import permits were prepared for tissue sample shipment from the Philippines to the California Academy of Sciences in San Francisco, California.

A minimum of eight to ten X. testudinaria per dive site were targeted for study, with a sponge fragment of approximately 20 grams collected using an apple corer following the protocol of Subagio et al. (2017) (Figs. 3 and 5). All sponges were photographed in situ with a digital camera. Habitat and depth were recorded (Fig.3) along with the sponge’s external morphology: body circumference, osculum diameter, and barrel height were recorded for all 95 sponges, spanning a broad range of sizes present in the population

(McMurray, 2008). After each dive, samples brought to the surface were stored in a basin with cold seawater for immediate transport to and examination at the field laboratory.

Sponge tissues for DNA extraction were stored in 95% ethanol and placed in a cool box.

Swierts et al. (2013) and Lopez-Legentil (2009) categorized the main morphotypes (Fig.

6) as “digitate” (D), “lamellate” (L), “smooth” (S) and “intermediate” (I) for X. testudinaria. I used their criteria to assign sponges in my own study areas to these categories. Individuals that were too small to be assigned to a morphotype were classified as “undetermined” (U). SPSS Statistics v.26 statistical analysis was used to determine all statistical analysis of my data. Analysis of means was done in Excel. In order to compare

8 means. One-way ANOVA was used with statistics descriptive effects homogeneity w^th a

Tukey Alpha (.05) post hoc test.

Figure 2. Field collection sites in Mabini-Tingloy and Isla Verde in Batangas Province - red line points to location of study area in the Philippines: (A) Pinnacle, (B) San Agapito, (C) Mainit, (D) Dead Palm. (E) Cathedral, (F) El Pinoy, (G) Devil' s Point, (H) Bethlehem, (I) Sombrero, (J) Beatrice. Map Sourcc: Google Earth (2018).

Figure 3, Sponge fragments being taken using an apple corer (left) and ecological parameters being recorded (right).

9 Figure 4. Sponge body circumference (left) and osculum diameter (right) being measured and recorded.

Figure 5. Image of sponge sample taken with an apple corer. On the left side is the outer layer (exterior) of the barrel sponge and on the right is the internal wall of the osculum. Notice the small channels in the middle of the core, where the food are filtered. The coloration on both ends of the samples are caused by the cyanobacteria symbionts.

10 Figure 6. Morphological types of Xestospongia testudinaria in the Verde Island Passage: (A) Digitate, (B) Smooth. (C) Intermediate, (D) Lamellate. Magnified views of the morphotypes: (E) Smooth, (F) Lamellate, (G) Intermediate. (H) Digitate.

Identification of Sponge Morphological Types

A classification of barrel sponges was based on the morphological types described in Swierts et al. (2013) and Lopez-Legentil et al. (2008) was used by adopting the following criteria:

11 • digitate sponges have digitate or spiky projections covering their outer body

surface.

• lamellate sponges have closely spaced, pronounced and smooth flanges extending

from the base to the apex of its exterior.

• smooth sponges lack significant surface projections.

• intermediate sponges have a rough surface area in which no distinct pattern is

detectable.

Slide Preparation, Spicule Measurement and Scanning Electron Microscopy

Following the laboratory method of Hooper (2003), spicule slides were prepared in the laboratory involving bleach digestion, which provides faster way to dissolve tissues but makes only temporary preparations. Sponge fragments were placed in vials to which was added household bleach (approximately 5% sodium hypochlorite) until the fragment was submerged. Tissue was left to be dissolved completely for an hour so that the spicules settled to the bottom of the vial. Spicules were then washed with distilled water and then with ethanol three more times. Samples were left for two hours to allow the spicules to settle again before placing on a glass microscope slide with the aid of a medicine dropper.

A cover slip was used to cover the sample, and a small amount of clear coat of nail polish was gently applied to all comers of the cover slip, to ensure a seal. This was left to air dry.

An Olympus CH30RF-100 compound microscope with an eyepiece graticule was calibrated and used to measure the length and width of 25 randomly chosen individual

12 spicules (Fig. 7) from each sponge. Four quadrats on each preparation were designated as areas from which to measure spicules so that, no spicules were measured twice.

Figure 7, Spicule length measured with a calibrated eyepiece graticule.

13 Figure 8. SEM image of oxea spicule variation found inX. testudinaria Individuals found in the VIP.

Additional spicules were examined with the Scanning Electron Microscope (SEM) using the following method. Spicule subsamples were placed on a labeled aluminum stubs to which adhesive conductive tabs had been applied. Small drops of spicules in ethanol prepared by the bleaching method described above were placed onto the tab and the stub air dried for at least two hours. Once dry, the samples were sputter coated with gold and palladium combination using a Cressington Sputter Coater 108.

A Hitachi SU3500 Scanning Electron Microscope was used to observe and photograph all the spicules encountered on each stub. An example of these photographs, all of which were curated for later examination, is shown in Figure 8.

14 AIM 2:

To test for differences in mitochondrial COI genetic composition among the morphotypes of X. testudinaria.

Rationale:

DNA analysis, in addition to morphological taxonomic characters, can provide comprehensive analysis of intraspecific variation among the barrel sponge samples.

Methodology:

Molecular Work: DNA Extraction, PCR, Gel Electrophoresis, PCR Purification-

ExoSAP-IT, Cycle Sequencing and Ethanol Precipitation.

X. testudinaria fragments from the Philippines were brought to the Center for

Comparative Genomics at the California Academy of Sciences for DNA sequencing.

Additional reference data for X. testudinaria were obtained from NCBI GenBank

(http://www.ncbi.nlm.nih.gov) and the Sponge Barcoding Project website

(www.spongebarcoding.org).

Eighty-nine sponge samples were preserved in 95% ethanol and dried on a paper towel to remove excess alcohol in the tissue. A total of 25 grams of tissue was cut off from the sponge sample. Total genomic DNA was extracted following the Qiagen DNeasy Spin

Column Protocol for Tissues. In order to determine extracted DNA quantity,

15 NANODROP 2000C software was used. The sample was first gently mixed to obtain an accurate concentration reading. This was done by raising the arm of the Nano drop instrument and adding 2 pL of DNA extract onto the pedestal and running the machine.

The ideal DNA concentration for PCR (between 25-100 ng/pL) was determined using the

Nanodrop DNA/RNA curve found here:

(http://www.biomedicalgenomics.org/RNA quality control.html)

The result from this procedure was exported to an Excel file. A fragment of the mtDNA

COI gene was amplified using the universal primers LC01490: 5'- ggtcaacaaatcataaagatattgg-3' HC02198: 5'-taaacttcagggtgaccaaaaaatca-3', described in

Folmer et al. (1994). I followed three main PCR steps: Denaturing, Annealing and

Extension, using a recipe for 25 pL PCR reactions in strip tubes using the INVITROGEN

Taq system with the following reagents: H2O, lOx Invitrogen Buffer, 50mM MgCh, 10 pM LC01490 primer, 10 pM HC02198 primer, 10 mg/mL BSA, lOmM dNTPs, 5p/pL

Invitrogen Taq and 2 pL of DNA. The PCR profiles consists of an initial denaturing step

(94°C for 30 sec), annealing (50°C for 30 sec) and extension (72°C for 45 sec) repeating

40 times, with a final extension step (72°C for 10 min) and infinite hold at 8°C, executed in a MyCycler BioRad PCR machine. After the PCR process, the quality and quantity of product was checked with gel electrophoresis by adding 2 pL of blue dye (4x) to 3 pL of

PCR product using a multichannel pipette onto the wells in a gel box. Two pL of 100 bp ladder was added to the first well. The gel box was covered and the voltage was set to 100 volts and the gel run for 26 minutes. Separation took place on a 1.5% agarose gel, after which the gel was photographed.

16 After gel electrophoresis, the PCR product was processed by the ExoSAP-IT method. Clean, labeled PCR tubes were prepared while the PCR products spun and uncapped in a vortex mixer (fisher scientific). These amounts were added to the diluted

ExoSAP-IT enzyme to the clean, labeled tubes. Using a repeater pipette, 2.0 fj.L of the enzyme was added to each tube. A multi-channel pipette was used to transfer 5 nL of PCR product to the tubes containing the ExoSAP-IT enzyme. The gel band image was checked to see if the bands were strong or faint. For faint bands, only 1.0 (J.L were used, while 2.0 p.L were used for strong bands. After the enzyme and the PCR product were combined, the caps were placed back on before a brief centrifuge.

The thermocycler was pre-heated at 95°C before placing the strip tubes following the pre-programmed ExoSAP-IT (incubation at 37°C for 30 minutes, incubation at 80°C for 15 minutes and hold time at 8°C) into the machine. For the cycle sequencing master mix, water, 5x big dye buffer, 10 |xM primer, big dye 3.1 and DNA were prepared. The master mix computed value was based on the calculations adding the reagents in the order listed (Table 1). An aliquot of 8.0 p,L of the master mix was added to each of the clean labeled-strip tubes with the use of an electronic repeat pipette before adding 2.0 jxL of the

ExoSAP-IT DNA, the tubes were capped and brief centrifuge spin was performed before placing the strip tubes in a PCR machine and run.

17 Table 1. Recipe sample for a 25 |iL PCR reaction. Stock 25 (it rxn H20 16 10X Invi'n Buffer 2.5 50 mM MgC12 0.75 10 fxM Primer LC01490 1 10 nM Primer HC02198 1 10 mg/mL BSA 1 lOmMdNTPs 0.5 5.0 u/uL Invi'n Taq 0.25 DNAfctL) 2

After cycle-sequencing, all sponge samples were transferred to a plate for ethanol precipitate by adding 2.5 |iL of 125 mM di-Na EDTA, pH 8.0, to the bottom of each well using the multi-channel pipette. Thirty |iL of 100% ethanol was added to each well, caps were put back onto the strip tubes, which were vortexed and set in a dark area for 15 minutes at room temperature for precipitation. After precipitation, the plates were placed in a Eppendorf 581 OR cold centrifuge machine with a first spin of 3000 x g (ref) for 30 minutes. After the spin, the tubes were immediately removed from the centrifuge taking care to avoid dislodging the pellets. Caps were gently removed, and the sample inverted onto a clean paper towel. A second spin was performed at 200 x g (ref) for 2 minutes with an inverted plate. Sixty |iL of ethanol were added to each well using a multichannel pipette after the spin a third spin was done at 2000 x g (ref) for one hour. After the hour-long spin, the plate was immediately removed, uncapped and gently inverted on a clean paper towel with a final spin of 200 x g (ref) for only 2 minutes. After the spin, the plate was taken out of the centrifuge and placed in an incubator for 8 minutes at 65°C.

18 Preparing Sequence Reactions for the ABI Prism 3130

Formamide was left to thaw fully before use. Ten |iL of formamide were added to

each of the samples using an electronic repeat pipette. Each tube was examined for bubbles, which were removed by gently tapping the tube.

The labeled strip tubes were placed in a pre-heated MyCycler BioRad PCR machine

(94-96°C) for 2 minutes to denature the pellets. The strip-tubes were placed immediately on an ice-cold rack and allowed to cool for 5 minutes. After cooling, the strip tubes are placed in a labeled ABI sequencing cassette sealed with a clean plate septa. The cassette was submitted to the lab manager for loading on the ABI Prism 3130 so that the Excel file on the desktop of the 3130 computer and the cassette’s label matched.

i

Mitochondrial Data Analysis and Interpretation

Sequences obtained from the ABI Prism 3130 were transferred to Geneious 11.1.5 to generate a consensus sequence, which was then aligned using MAFFT. The total sequence length was 658 base pairs. The data were saved in nexus file format for insertion into the Mesquite program, which was used to check the final alignments. These were exported back to Geneious using Phylip format and uploaded to CIPRES Science Gateway versionv 3.3 to run PartitionFinder2 on XSEDE and Mr. Bayes. The phylogenetic tree generated was opened using Fig Tree or iTOL. The best BLAST searches of GenBank for

19 COI were sequences from Xestospongia muta with 99-100% identical sequence (NCBI

Blast data: MH29745.1, MH285810.1, and MH285806.1). These were chosen to serve as outgroups in generating the final phylogenetic tree. Additional outgroups from the same family, Petrosiidae, were added from the genus sp. (JN242215.1, JN242216.1,

JN242220.1). Sponge haplotypes were determined by using the COI data from Geneious and exported as fasta file then uploaded to DNAsp version 5 and ABGD, respectively.

RESULTS

A. Morphological Types

The most abundant morphological types among 95 Xestospongia testudinaria sponges sampled in the Verde Island Passage (VIP) were the lamellate (n=63), followed by digitate (n=22), intermediate (n=8) and smooth (n=2). Three out of the four morphotypes occurred in close proximity to each other and were present in a majority of the ten dive sites. Individual variations were observed, given the distribution of all these morphotypes in one dive site. Among the 10 dive sites, only Devii's Point was found to have all four morphological types present, followed by Dead Palm, Mainit, Beatrice, and

Cathedral, each having three morphotype characters present, while Pinnacle, San Agapito,

El Pinoy and Bethlehem recorded only two morphotypes (Fig. 9 and Fig. 10). All the barrel sponges collected were observed to have variations in their maroon red coloration, but the reason for this coloration was not identified and not part of the project.

20 MORPHOTYPES PER DIVE SITE

10 9 8 7 6

■ LAMELLATE ■DIGITATE ■INTERMEDIATE ■ SMOOTH

Figure 9. Distribution of morphological types at each dive site.

LEGEND:

L = Lamellate

L D = Digitate u e u l . 5 - 1 k I = Intermediate L D^I i?D , i V S = Smooth l ; d

A. D. 1, S

L.D q

It! Google Earth pas LKi.1**nth, r-i ? -3* trie SO. S.nrff, Iraje i- oUi 3 Vsrar fectntoprr Figure 10. Map of morphotype distribution. (A) Pinnacle. (B) San Agapito, (C) Mainit, (D) Dead Palm, (E) Cathedral, (F) El Pinoy, (G) Devil s Point, (H) Bethlehem, (I) Sombrero, (J) Beatrice. Map Source: Google Earth (2018).

21 T able 2. One Way ANOVA analysis of mean body height for each dive site.

ANOVA

HEIGHT Sum of Squares df Mean Square F Sig.

Between Groups 10637.983 9 1181.998 1.293 .253

Within Groups 77723.175 85 914.390

Total 88361.158 94

Table 3. One Way ANOVA analysis of mean sponge osculum diameter per dive site.

ANOVA

OSCULUM Sum of Squares df Mean Square F Sig.

Between Groups 5661.822 9 629.0S1 2.532 .013 Within Groups 21119.825 85 248.469 Total 26781.647 94

Table 4. One Way ANOVA analysis of mean sponge body circumfercncc per dive site.

ANOVA

CIRCUMFERENCE Sum of Squares df Mean Square F Sig.

Between Groups 185118.860 9 2 0 5 68 .7 62 5.884 .000 Within Groups 2 90 13 2.83 9 83 3 495.576 Total 4 75 25 1.69 9 92

2 2 The statistical analysis shows that sponge circumference and osculum diameter have significant differences among dive sites while length showed values above the p-value of 0.05, indicating no significant differences in sponge height among dive sites.

SOMPPERO oW 240.00 wz 35 220 00 SAN AGAPITO

CJ 200 00 0£ BEATRICE o Q> 180.00 O • BETHLEHEM (fl EL PINOY* HI 160.00 C9 MAINIT oZ 8> 1«* o c DEVILS POINT «IS 120.00 PINNACLE

DEAD PALM 100.00 CATHEDRAL'

1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 DIVESITES

Figure 11. Mean sponge body circumference (cm) per dive site.

23 BEATRICE SAN SGAPITO

HI O 80 Ul _ l CATHEDRAL Q> O m SOMBRERO* BETHLEHEM, oU l 70.00 z MAINIT o 0. PINNAClE OT DEAD PALM c 10 DEVILS POINT

EL PINOY

1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 DIVESITES

Figure 12. Mean sponge body height (cm) per dive site.

50.00 SOMBRERO

BEATRICE

D O OT O Ul O z 35.00 MAINIT POINT o DEVILS POINT 0. OT BETHLEHEM CATHEDRAL c SAN AGAPITO •>H EL PINOY DEAD PALM

PINNACLE

1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 DIVE SITES

Figure 13. Mean sponge osculum diameter (cm) per dive site.

24 Sponges at Sombrero were larger in terms of circumference and osculum diameter but not in body height. Beatrice had the tallest barrel sponges and San Agapito had the next tallest and widest barrel sponges. Sponges with the widest osculum diameter were found at Sombrero followed by Beatrice and Mainit. Pinnacle had the smallest osculum diameters, with El Pinoy having the lowest sponges and Cathedral having sponges with the smallest circumference of all (Figs. 11, 12, and 13).

B. Spicule Measurements

The One Way ANOVA anal vs is conducted on 95 sponge samples with 2,375 individual spicules measured for length and width, showed significant differences (Table

5; among dive sites. A Tukey’s post hoc test was applied to both spicule length and width with a P-value of alpha = 0.05 (Table 6 and Table 7). This test revealed significant differences in spicule length among Devil’s Point, Dead Palm, and Cathedral, while the spicule width showed significant differences among Sombrero, Dead Palm, and Devil’s

Point. All the other sites not mentioned showed no significant differences in either measurement.

Table 5. One Way ANOVA analysis of mean spicule length and width per dive site.

ANOVA

Sum of Squares df Mean Square F Sig.

LENGTH Between Groups 18215.261 9 2023.918 3.48* 001 Within Groups 49373.111 85 580.860 Total 67588.372 94 WIDTH Between Groups 329.731 9 36 637 6 348 .000 Within Groups 490.532 85 5.771 Total 820.263 94

25 Table 6. Tukey’s post hoc test (spicule length).

SPICULE LENGTH

Tukey HSDa b Subset for alpha = 0.05 DIVESITES N 1 2 SOMBRERO 10 331.6800 BETHLEHEM 10 349.3800 349 3800 EL PINOY 10 353.0400 353.0400 BEATRICE 10 355.0000 355 0000 PINNACLE 8 356.2500 356.2500 SAN AGAPITO 10 358.6800 358.6800 MAINIT 10 362.5400 362.5400 DEVILS POINT 8 365.8250 365.8250 CATHEDRAL 9 376.0000 DEAD PALM 10 383.8000 Sig. .079 .074

Table 7. Tukey’s post hoc test (spicule width).

SPICULE WIDTH

Tukey HSDa b Subset for alpha = 0.05

DIVESITES N 1 2 3 SOMBRERO 10 12.4680 BEATRICE 10 12.9400 12.9400 BETHLEHEM 10 14.6680 14.6680 EL PINOY 10 14 6800 14.6800 MAINIT 10 14.7160 14.7160 CATHEDRAL 9 15,2889 15.2889 PINNACLE 8 15.4200 15.4200 SAN AGAPITO 10 15.6860 15.6860 DEVILS POINT 8 16.1500 16.1500 DEAD PALM 10 19.5200 Sig. 120 122 .085

26 450.0 400.0 350.0 m a m — 300.0 250.0 200.0 355.0 3494 331.7 3658 376,0 150.0 100.0 50.0

Figure 14. Mean sponge spicule length (fxm) per dive site.

25.0

20.0 19.5

15.0 > k 1J.3 1 4 7 1 1 7 i4‘ 1 * 9 10.0

5.0

0.0

S

Figure 15. Mean sponge spicule widlh (nm) per dive site.

Dead Palm had the greatest spicule length and width measurements for all u.ve sites- with Cathedral having the second-longest spiculcs, followed by Devil’s Point. Devil’s

Point had the second thickest spicules of all sites, followed by San Agapito and Cathedral.

27 Sombrero and Beatrice registered the lowest mean spicule width (Figs. 14 and 15). Spicules of X. testudinaria in the Verde Island Passage were generally of megascleres tangential type or curved spicules oxea with no microscleres (Fig. 16).

Figure 16. SEM image of oxea spicule variation found in Xestospongia testudinaria individuals in the VIP.

As discussed below, four haplotypes were found during the molecular analysis.

Morphological traits were analyzed in the context of these haplotypes. Spicule lengths and widths of haplotypes that were found at the same dive sites were statistically analyzed using

One-Way ANOVA (Table 8 and fable 10). El Pinoy and Dead Palm were chosen for this analysis because of the number of different haplotypes present (Fig. 21).

Table 8. One Way ANOVA ofX. testudinaria spicule length and width from El Pinoy. AN OVA

Sum of Squares df Mean Square F Sig. LENGTH Between Groups 154289.600 9 17143.289 11.596 .000

Within Groups 354800.000 240 1478.333 Total 509089.600 249 WIDTH Between Groups 944.400 9 104 933 4.176 .000 Within Groups 6030.000 240 25,125 Total 6974.400 249

28 Table 9. Tukey test ofX. testudinaria spicule length and width from El Pinoy.

Multiple Comparisons Tukey HSD Mean Difference (1- Dependent Variable (1) ELFINOY (J) ELPINQY J) Std. Error Sig. LENGTH 1 2 -27.20000 10.87505 .273 HAPLOTYPE 1 3 HAPLOTYPE 3 53.20000' 10.87505 .000 4 14.00000 10.87505 .956 5 HAPLOTYP 3 47.60000' 10.87505 .001 6 18.00000 10.87505 .819 7 HAPLOTYPE 4 2.40000 10.87505 1.000 8 -25.20000 10.07505 .302 9 4.40000 10.87505 1.000 10 10.40000 10.87505 .994 2 1 27.20000 10.87505 .273 HAPLOTYPE 1 3 HAPLOTYPE 3 80.40000" 10.87505 000 4 41.20000* 10.87505 .007 5 HAPLOTYPE 3 74 aoooo' 10.07505 .000 6 45.20000' 10.87505 .002 7 HAPLOTYPE 4 29.60000 10.87505 .171 8 2.00000 10.87505 1.000 9 31.60000 10.87505 .110 10 37.60000' 10.87505 .022

W IDTH 1 2 2.40000 1.41774 .799 HAPLOTYPE 1 3 HAPLOTYPE 3 6.20000" 1.41774 .001 4 2.60000 1.41774 713 5 HAPLOTYPE 3 5 800QQ* 1 41774 .002 6 2.80000 1.41774 .617 7 HAPLOTYPE 4 1.00000 1.41774 .999 8 .80000 1.41774 1.000 9 4.00000 1.41774 .135 10 3.60000 1.41774 .253 2 1 -2.40000 1.41774 799 HAPLOTYPE 1 3 HAPLOTYPE 3 3.80000 1.41774 .188 4 .20000 1.41774 1.000 5 HAPLOTYPE 3 3.40000 1.41774 .332 6 .40000 1.41774 1.000 7 HAPLOTYPE 4 -1.40000 1.41774 .993 8 -1.60000 1.41774 .981 9 1.60000 1.41774 .981 10 1.20000 1.41774 .998

29 As shown in Table 9, Tukey’s post hoc test for El Pinoy representatives showed that sponge one of Haplotype 1 has a significant difference in spicule length from

Haplotype 3 but showed no significant difference from Haplotype 4. Sponge two

Haplotype 1 showed the same significant difference from Haplotype 3 and no significant difference from Haplotype 4. Spicule width, on the other hand, showed no significant differences for all 3 haplotypes present in the dive site.

Table 10. One Way ANOVA of Xestospongia testudinaria spicule length and width from Dead Palm. ANOVA

Sum of Squares df Mean Square F Sig.

LENGTH Between Groups 65106.000 9 7234.000 14.186 .000 Within Groups 122384.000 240 509.933 Total 107490.000 249 WIDTH Between Groups 118.400 9 13.156 3.083 002 Within Groups 1024.000 240 4.267 Total 1142.400 249

One Way ANOVA analysis of Dead Palm spicule lengths and widths showed significant differences among samples (Table 10). Tukey’s post hoc test for Dead Palm spicule length showed Haplotype 1 is significantly different from Haplotype 4 but is not consistently different from Haplotype 3. Sponge two Haplotype 1, on the other hand, showed variable significant differences from Haplotype 3 but no significant difference from Haplotype 4. Spicule width showed no significant difference for all three haplotypes

(Table 11).

30 Table 11. Tukey test oiX. testudinaria spicule length and width from Dead Palm.

Multiple Comparisons Tukey HSD Mean Difference (1- Dependent Variable (I) DEADPALM (J) DEADPALM J) Std. Error Sig. LENGTH 1 2 -30.40000' 6.38707 .000 HAPLOTYPE 1 3 HAPLOTYPE 3 -20.00000 6.38707 .060 4 13.60000 6.38707 .509 5 HAPLOTYPE 4 -30.00000’ 6.38707 .000 6 HAPLOTYPE 3 -40.00000' 6.38707 .000 7 HAPLOTYPE 3 -5.60000 6.38707 .997 8 -32.40000" 6.38707 .000 9 HAPLOTYPE 4 -30.40000’ 6.38707 .000 10 -18.80000 6.38707 .100 2 1 30.40000’ 6.38707 .000 HAPLOTYPE 1 3 HAPLOTYPE 3 10.40000 6.38707 .833 4 44 OOOOO- 6.38707 .000 5 HAPLOTYPE 4 .40000 6.38707 1.000 6 HAPLOTYPE 3 -9.60000 6.38707 .890 7 HAPLOTYPE 3 24.80000" 6.38707 .005 8 -2.00000 6.38707 1.000 9 HAPLOTYPE 4 .00000 6.38707 1.000 10 11.60000 6.38707 724

WIDTH 1 2 -.40000 .58424 1.000 HAPLOTYPE 1 3 HAPLOTYPE 3 -.40000 .58424 1.000 4 1.20000 .58424 562 5 HAPLOTYPE 4 -.40000 .58424 1.000 6 HAPLOTYPE 3 .00000 .58424 1.000 7 HAPLOTYPE 3 -.40000 .58424 1.000 8 -.40000 .58424 1.000 9 HAPLOTYPE 4 .00000 .58424 1.000 10 1.60000 .58424 .164 2 1 .40000 .58424 1.000 HAPLOTYPE 1 3 HAPLOTYPE 3 .00000 .58424 1.000 4 1.60000 .58424 164 5 HAPLOTYPE 4 .00000 .58424 1.000 6 HAPLOTYPE 3 .40000 .58424 1.000 7 HAPLOTYPE 3 00000 .58424 1.000 8 .00000 .58424 1.000 9 HAPLOTYPE 4 .40000 58424 1.000 10 2.QQQQQ' .58424 .025

31 C. Mitochondrial DNA Analysis

To better understand the haplotype distribution of X. testudinaria within the Verde

Island Passage, a total of 89 sponge COI mitochondrial genes were sequenced and yielded

4 different haplotypes (Table 12) through DNAsp with confirmatory Automatic Barcode

Gap Discovery (ABGD) analysis. An initial partition with prior maximal distance P=1.00e-

03 Distance JC69 Jukes-Cantor MinSlope=0.500000 showed that Haplotype 1 was the most abundant (n=79), and was found at all dive sites, while Haplotype 3 (n=5) was limited to two dive sites (El Pinoy and Dead Palm). Haplotype 2 (n=4) was found at three dive sites (Beatrice, Dead Palm, and El Pinoy), but Haplotype 4 (n=l) was found at only one

(Sombrero). Images of the haplotypes with their corresponding morphotype identified in the Verde Island Passage are shown in Fig. 17.

Table 12. Haplotypes ofX testudinaria from the Verde Island Passage, Philippines. HAPLOTYPE FREQUENCY SEQUENCES

(Geneious data in fasta file)

1 79 1-5 7-31 33-37 39-49 53-79 81-89

2 4 6, 34, 38, 52

3 5 32, 35, 36, 50,51

4 1 80

32 HAPLOTYPE 1

Beatrice 9 (Intermediate) Beatrice 8 (Lamellate)

Beatrice 7 (Lamellate) Beatrice 5 (Digitate)

Beatrice 4 (Lamellate) Beatrice 3 (Lamellate)

Beatrice 2 (Digitate) Beatrice 1 (Lamellate)

33 HAPLOTYPE 2

Devils Point 4 (Smooth) Cathedral 3 (Lamellate)

HAPLOTYPE 3

El Pinoy 5 (Lamellate) El Pinoy 3 (Lamellate)

Dead Palm 7 (Digitate) Dead Palm 6 (Digitate)

Dead Palm 3 (Lamellate)

34 HAPLOTYPE 4

El Pinoy 7 (Lamellate) Dead Palm 9 (Intermediate)

Dead Palm 5 (Intermediate) Beatrice 6 (Lamellate)

Figure 17. Images of the four haplotypes of Xestospongia testudinaria found in the VIP.

35

s . > K v ‘ ’

C x S 4 1 < . 0 * 0"Hf, ' - •*>«

D*vfc**<*iM_Smoort, ,uwa>*«,*w ^a*^***IUt» * Z t z * *^9 *<° • 'aW > * * y y '

j. VAW <*, '% ««. w v \ v

Figure 18. Rooted phylogenetic tree based on mitochondrial COI DNA of Xestospongia testudinaria from the Verde Island Passage with corresponding morphotypes. Outgroup is highlighted by green line (Petrosia sp.).

36 CathedralBj^aniell ate

ElPinoySj-amellate

ElPinoy3>mBTIate

o „ * > + # P + * *

o *

D*

O * ^

Figure 19. Magnified portion of rooted phylogenetic tree shown in Fig. 18, with corresponding morphoiypes and haplotypes 2 -3 (large numbers).

37 \V % *'C'o V

Jtfir *

% * , ****** **»*.***•»,4 *''•*. “H ^ **/ >'/,> AA V V *

••-•-.U..C a ctRiftoy7_Ljm«ll«t* •f^} 0#«dP*lm00jnt»rmtdi»tt •

Figure 20. Rooted phylogenetic tree based on mitochondrial COI DNA of X. testudinaria from the Philippines, including Caribbean, Myanmar, Viet Nam and the Red Sea. Green line indicates the outgroup.

38 CARIBBEAN M YANM AR PHILIPPINES RED SEA o (Fig. 18) aligned with C5 n > H SB m r 1 2 3 4 HAPLOTYPE LEGEND: Xestospongia muta, 39 EL PINOY 5 DEAD PALM3 DEAD PALM6 EL PINOY3 DEAD PALM 7

EL PINOY7 DEAD PALM9 BEA TRICE6 DEAD PALM5 V IET NAM

-o w H so o -c t/5 c/i One of the two outgroups added, o Haplotype 1, revealed little nucleotide divergence and shares the same morphological Figure 21. Phylogenetic haplotype tree representation OUTGROUP *o m H » -o V! CZ> O

PETROSIA SP.3 u characters. Interestingly, the majority of barrel sponges that branch separately from

Haplotype 1 were from Dead Palm, Devil’s Po it, Cathedral, El Pinoy, and Beatrice. It was noted that all four morphotypes (lamellate, digitate, intermediate and smooth) were represented in all four haplotypes. Sponge 4 of Devil’s Point and sponge 3 of Cathedral

landed in the same haplotype but differed in morphological characters (Fig. 18).

In order to better understand haplotype distribution within Xestospongia, another phylogenetic tree was created with GenBank sequence data from Myanmar, Viet Nam, the

Red Sea, and Caribbean barrel sponges, which were all aligned with the Philippine COI

sequence data (Fig. 20) to test for haplotype similarities. The results revealed that out of the four haplotypes found in the Philippines, Haplotype 4 shared the same clade with haplotypes from Viet Nam. Haplotype 3 remained the same from the first phylogenetic tree constructed (Figs. 20 and 21). with samples mostly from Dead Palm and El Pinoy. This haplotype appears to be unique to the Philippines. Haplotype 2 is unique to the Philippines, composed of only two samples, and Haplotype 1 remained the most strongly represented of all four haplotypes. This haplotype is widespread geographically and includes specimens

from the Indo-Pacific, the Caribbean, the Red Sea, and Myanmar (Fig. 21).

DISCUSSION

The findings concerning the morphotypes and haplotypes of X. testudinaria in the

VIP contrast with the findings from Indonesia. Swierts et al. (2013) found four distinct

morphotypes around Lembeh Island, dominated by digitate growth forms. According to

Swierts et al. (2013), the digitate morphotype was abundant in waters adjacent to harbor

40 buildings and other developed areas of Bitung City, suggesting that the digitate morphotype

is favored in turbid waters with high nutrient values caused by shipping traffic and human

activities. The next most abundant forms in Indonesia were smooth, intermediate and

lamellate types respectively. Kelly-Borges and Bergquist (1988) said that different

functional roles have been proposed to explain the different morphological types and that

the lamellate form is linked to more efficient removal of particulates in the water column.

While conducting fieldwork in the VIP, a well-known tourist dive spot noted for the clarity

of its waters, it was noted that the majority of the dive sites were dominated by lamellate

growth forms. This suggests that the VIP is a lot cleaner compared to Indonesian sample

sites, overall, because the lamellate form seems to be an adaptation of sponges to increase

feeding surface area to extract more nutrients from the water column. The digitate morphotype was the second most dominant in the VIP. followed by intermediate and lastly,

smooth. Measurements of sponge circumference, osculum diameter and height were not recorded by Swierts et al. (2013) when they conducted their research onX. testudinaria in

Lembeh, Indonesia. My study is the first of its kind, because detailed morphological measurements were recorded and correlated with molecular results. It should be noted that measurements, including circumference, osculum diameter, and height showed great variation but that none of these characteristics was significantly different among sites, nor were they strongly correlated with different haplotypes.

The attempt to align the mitochondrial COI sequences from the Philippines with

Indonesia COI sequences taken from GenBank data (KC424439-KC424444) resulted in no alignment. The lack of overlap of my Philippine data with the data from Swierts et al.

(2013) was due to a different part of COI region the present study used, and new COI

41 primers were created for a different region (3’ prime) of the mitochondrial COI Swierts et

al. (2013) used to amplify their sequences (Fig. 22). The new COI primers (Folmer et al.,

1994) C1J2165 and ClNpor2760 were created, thus, the data provided by Swierts et al.

(2013) cannot be compared directly with the sequences recovered in the present study.

With a total of 126 COI sequences processed and analyzed, they found six different

haplotypes they named C1-C6, based on four variable sites.

>00X 1 02 (pos 674) I.C01480 (pos 90) CU 2l 6f (poo 752) F6gLC01490 (pos 50) fe ’COtpoiFf (poo 800) □ standard barcoding fragment r suggested extension C O IporR I (pos 1274 HC02169 (poo. 700) COX1-R1 (pot 1267) 0gHCO2lM (pot. 700) I C 1Npor2760 (poa 1297) ^ Figure 22. Schematic view of the COI standard barcoding region (Folmer et al. 1994), anc the primer used by Swierts et al. (2013).

Attempts were also made to align GenBank COI sequences of Xestospongia

testudinaria from Viet Nam, the Red Sea and Myanmar, and all of them align perfectly

with the Philippine COI data. A total of four haplotypes were recorded (Table 14). Two of the haplotypes found are unique to the Philippines and a third one is found only in Viet

Nam and the Philippines, suggesting that Haplotype 4 is the same haplotype found in Viet

Nam (Fig. 20). Haplotype 1 is widely distributed, indicating little or no genetic variation

among Caribbean and Indo-Pacific specimens of this haplotype. The uncorrected p-

distances showed 0.3% across all four haplotypes of all specimens examined suggesting

little to no difference among sponge samples. Nucleotide difference for the COI marker

42 was also analyzed to determine sequence position and determine proximity of the samples

(Table 13). Table 14 summarizes data from the three dive sites that were part of the ABGD

analysis (Fig. 21), compared to one another in relation to their morphotypes, haplotypes,

spicule lengths, and spicule widths. Dead Palm has 3 morphotypes and 3 haplotypes, in which sponges of the same haplotype but different morphotype were compared in terms of

spicule length and width. The results from comparing these same haplotypes vary.

Comparison with El Pinoy and Devil’s Point also showed variations in spicule length and width among the same haplotypes and morphotypes.

Table 13. Nucleotide difference for mitochondrial marker COI. mtDNA HAPLOTYPE COI SEQUENCE PC>SITION COI = 89 samples 222 235 318 HI (BEATRICE1) C CC H2 (DEAD PALM3) C c T H3 (BEATRICE6) c TT H4 (SOMBRERO 1) ACC

43 Table 14. VIP haplotypes in relation to morphotypes, spicule length, and spicule width. DEAOMIM (W fflW U P t KATiwlFf HHHHHNM W P l UiK bte I ! 1 0.000 0.060 MOO 0362 1.000 1.000 1.000 U 0 0 1.000 0.164 W! Lamcfate 1 0.000 0.833 0.164 1.000 1000 1.000 1.000 1.000 W i Lamcfate 1 0.600 0833j 1.000 1.000 1.000 1.000 1.000 0.015 W I n d i t e 1 0.509 0.000 O.OOC I 0.562 0.164 0.164 0.562 1.000 qmpS Intmedste i1 0.000 1.000 0.863 1.000 1.000 1.000 0.025 Wjtae 1 0.000 0.890 0.060 1.000 1.000 0.164 mi Diffate 1 1997 0.005 0.423 1.000 0.025 w i Dipbte I 0.000 1.000 0.641 1.000 0.973 0.002 1.000 0.309 1.000 0.025 W) Interawdbte -1 0.000 1.000 0.833 0.005 1.000 1.000 1.000 1.000 H 0.164 q m p lO Dipbte I 0.100 0.724 1.000 0.553 0.509 0.164 0.025 0.025 0.164 0 M 5 K W M M W IM HflflOiyPt HMBBBm N I w i M b i t 1 ■ [ 0 3 7 8 1.000 0.978 0.000 0.714 0.008 0375 W! lim h te 1 0.978 ■ i 0.955 1.000 0.405 0.964 1.000 0.000 0.714 0.008 0375 9 0 * 3 Smooth 1 1.000 0.955 ■ 0.955 0.000 0.000 OHOO 0.002 W' Smooth 1 0.831 1.000 1755 ] 1.000 0.000 0.942 0.991 0.604 w i Djpbte 1 0344 0.405 0.972 0D36 0.405 0.000 OHM 0.000 0.014 W i Umfate 1 0.465 0.964 0.376 0.964 0.002 & 6 0.491 0.998 w ? M fimifate 1 0.978 1.000 0.955 1.000 0.405 0.964 0.000 0.491 7 0.143 sponpS M a t t 1 0.000 0.0000.000 0.000 0.000 0.002 0.000 0.998 0.143 8 anNOT tw w rm Htfioim K l n t - t H w i O fM 1 ■ [hpT0.000 1.000 0382 1.000 0.994 0.617 0.999 1.000 1135 0253 W! 1 0273 2 (MHO 0.171 1.000 0.110 0022 1.000 0393 0.981 0 3(1 0.99! W l iM fe tl 3 OHOO o r a ] J 0.000 0.000 0.000 0.004 0.332 0111 0.007 0J69 0.713 iM tta t 1 0356 0007 M l i ] 0387 0014 0397 1.000 1000 0381 0.959 1993 0999 wi l a t a 3 OjOOI o.ooc 1.000 mn 0,(02 OHOO 0.004 0025 0332 ___ UK 0028 0.018 0359 0.869 w* l a t a t 0J19 1002 0.044 6 MIS 0.004 0.963 1.000 IK# 0332 1000 0.518 6 ■1W 0.923 0.998 1.000 l » t a 4 UOO 1171 0.000 0.915 7 0254 1.000 0.999 1993 aou 1981 0.028 0.959 7 1.000 0511 0.713 wl I n t o 1 0382 1000 0.000 0.004 0J54 0.171 0.007 0.959 0.923 1000 0.421 1617 w» Ij m Im 1 UOO 1110 OHOO 9 0.998 0518 0.421 ; M b n 1 0394 1022 0.004 UOO 1.000 0.713 0*17 UOO 10

Based on the findings of Setiawan et al. (2015), the three barrel sponges, Xestospongia testudinaria, Xestospongia muta, and Xestospongia bergquistia cannot be differentiated using the I3-M11 partition of coxl, which was suggested by Erpenbeck et al. (2006b) to be a candidate marker for species-level phylogenies other than the standard barcoding marker or by use of any morphological or ecological parameter alone. My research findings agree with global studies on barrel sponges, showing the vast majority of Xestospongia (including X. muta from the

Caribbean) are of the same haplotype. The lack of variation between the large haplotype of X testudinaria and X muta suggests that ^ muta from the Caribbean is the same

44 species as X. testudinaria. According to Setiawan et al. (2013), X testudinaria and X muta share many characteristics and are 99-100% identical in morphology and DNA sequence with a little divergence between them. According to Montalvo and Hill (2011), both of these species also displayed similar bacterial community compositions. These resemblances still prevail despite the fact that these two Xestospongia species are geographically distant and must have been separated for at least three million years, since the Isthmus of Panama closed (Keigwin, 1978). Interestingly, the study of Swierts et al.

(2013), along with the present study, shows that there is greater variation in haplotypes within Indonesia and the Philippines than between these areas of the Coral Triangle and the rest of the world. This strongly suggests that the Coral Triangle represents a hot spot of diversity generation as has been suggested previously for other taxa (Carpenter and

Springer, 2005; Briggs, 1999; Hoeksema, 2007; Bellwood & Meyer, 2009; Hooper and

Levi, 1994; Van Soest, 1994).

In summary, my research has innovatively combined morphology with molecular data, dealing with morphological characters that differentiate the different morphotypes found at the collection sites. I measured the osculum diameter, the sponge body circumference, sponge body length, as well as spicule length and width. These parameters were compared with a molecular analysis using the mitochondrial COI gene. The statistical analysis conducted for the spicule measurements, morphotypes, body circumference, body length, and sponge osculum diameter, were integrated with the COI mitochondrial results to determine if there is a common haplotype pattern within samples from a dive site and between dive sites. The results were inconsistent in comparing the haplotypes found at one

45 dive site. The analysis was unable to establish a pattern that could refute or support the morphological concept of Xestospongia classification and no morphological features examined correlated well with the genetic divergence that was evident among morphotypes. Setiawan et al. (2013) suggested that the arrangement of spongin skeleton and chemical composition of Xestospongia supported the existence of distinct species, but in the present study, an analysis was done to compare haplotypes with its morphology, spicule measurements and mitochondrial COI sequence, showed that these features were not robustly associated with haplotypic variation.

To further understand the overall morphological and molecular relationship of barrel sponges in the Philippines and globally, future research should include collection of more samples to test whether there are morphological differences in colony form that relates to the four distinct haplotypes discovered in this study. Adding another mitochondrial gene, ATP6 , which has shown more variation than COI, may also prove useful in resolving these systematic issues. Lastly, it will be necessary to conduct genetic and morphological sampling of other Xestospongia that appear to represent distinct species in WoRMS, to determine whether these represent distinct species or not.

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51 APPENDIX 1

Appendix 1. P-distance values ofX. testudinaria in the VIP.

105 Beatrice3 Lamellate 0.000000 0.000000 0.001775 0.003555 0.000000 0.000000 0.000000 0.000000 0.001794 0.000000 0.000000 0.000000 0.003555 0.000000 0.000000 0.001788 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.003586 0.001781 0.000000 0.000000 0.000000 0.001775 0.003555 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.001775 0.000000 0.000000 0.000000 0.001775 0.001775 0.000000 0.003555 0.001775 0.001775 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.001775 0.001775 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.001775 0.000000 0.000000 0.003555 0.003555 0.003555 0.003555 0.003561 0.003555 0.000000 0.000000 0.000000 0.092337 0.102432 0.110606 e5 Digitate 0.000000 0.000000 0.001625 0.1 0.000000 0.000000 0.000000 0.000000 0.001741 0.000000 0.000000 0.000000 0.003427 0.000000 0.000000 0.001797 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.003264 0.001703 0.000000 0.000000 0.000000 0.001625 0.003254 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.001625 0.000000 0.000000 0.000000 0.001625 0.001625 0.000000 0.003254 0.001625 0.001625 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.001625 0.001625 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.003254 0.000000 0.000000 0.003555

52 0.003555 0.003555 0.003555 0.003580 0.003561 0.000000 0.000000 0.000000 0.091134 0.101087 0.107124 Bethlehem 1 Lamellate 0.001775 0.001625 0.000000 0.001747 0.001706 0.004800 0.001625 0.001617 0.003486 0.001688 0.001683 0.001788 0.005119 0.001794 0.001801 0.003580 0.001753 0.001562 0.001625 0.001882 0.001641 0.007853 0.001625 0.001657 0.001641 0.001641 0.001594 0.001641 0.001625 0.001697 0.001688 0.000000 0.001628 0.004902 0.003264 0.003197 0.001531 0.001531 0.000000 0.004601 0.001531 0.001531 0.003069 0.001531 0.001533 0.001655 0.001531 0.007684 0.000000 0.001531 0.001531 0.014470 0.000000 0.003064 0.001531 0.004601 0.003064 0.003064 0.001531 0.001594 0.001533 0.001531 0.001531 0.001531 0.001531 0.001531 0.000000 0.003064 0.001531 0.001531 0.001531 0.001531 0.001531 0.001531 0.001531 0.000000 0.000000 0.001531 0.001533 0.001531 0.001531 0.001531 0.001584 0.001531 0.001579 0.001594 0.001576 0.001594 0.001589 0.001987 0.004601 0.003181 0.002187 0.005000 0.005000 0.005000 0.005000 0.005034 0.005008 0.001697 0.001697 0.001685 0.092254 0.100149 0.108127 Bethlehem9 .amellate 0.003555 0.003517 0.001747 0.000000 0.003517 0.003499 0.003517 0.003499 0.005310 0.003499 0.003499 0.003580 0.007013 0.003593 0.003606 0.005376 0.003511 0.003499 0.003517 0.003769 0.003555 0.003499 0.003517 0.003593 0.003555 0.003555 0.003499 0.003555 0.003517 0.003505 0.003499 0.001797 0.003523 0.007076 0.005272 0.003499 0.003499 0.003499 0.001747 0.007013 0.003499 0.003499 0.003499 0.003499 0.003505 0.003517 0.003499 0.003499 0.001747 0.003499 0.003499 0.003499 0.001747 0.005254 0.003499 0.007013 0.005254 0.005254 0.003499 0.003499 0.003499 0.003499 0.003499 0.003499 0.003499 0.003499 0.001747 0.005254 0.003499 0.003499 0.003499 0.003499 0.003499 0.003499 0.003499 0.000000 0.000000 0.003499 0.003499 0.003499 0.003499 0.003499 0.003499 0.003499 0.003499 0.003499 0.003499 0.003499 0.003499 0.003979 0.005254 0.006376 0.004380 0.007088 0.007088 0.007088 0.007088 0.007139 0.007101 0.003499 0.003499 0.003499 0.094745 0.098716 0.110757 rail Lamellate 0.000000 0.000000 0.001706 00.003517j 0.000000 0.000000 0.000000 0.000000 0.001741 0.000000 0.000000 0.000000 0.003427 0.000000 0.000000 0.001797 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.003427 0.001711 0.000000 0.000000 0.000000 0.001706 0.003415 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.001706 0.000000 0.000000 0.000000 0.001706 0.001706 0.000000 0.003415 0.001706 0.001706 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.001706 0.001706 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.003415 0.000000 0.000000 0.003555 0.003555 0.003555 0.003555 0.003580 0.003561 0.000000 0.000000 0.000000 0.091134 0.101087 0.107124 ral3 Lamellate! 0.000000 0.000000 0.004800 0.10.003499 0.000000 0.000000 0.000000 0.000000 0.001741 0.000000 0.000000 0.000000 0.003409 0.000000 0.000000 0.001788 0.000000 0.003197 0.000000 0.000000 0.000000 0.003197 0.000000 0.000000 0.000000 0.000000 0.003197 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.003264 0.005025 0.000000 0.003197 0.003197 0.004800 0.006407 0.003197 0.003197 0.003197 0.003197 0.003202 0.003468 0.003197 0.003197 0.004800 0.003197 0.003197 0.003335 0.004800 0.004800 0.003197 0.006407 0.004800 0.004800 0.003197 0.003335 0.001599 0.003197 0.003197 0.003197 0.003197 0.003197 0.004800 0.004800 0.003197 0.003197 0.003197 0.003197 0.003197 0.003197 0.003197 0.003679 0.004286 0.003197 0.003202 0.003197 0.003197 0.003197 0.003302 0.003197 0.003302 0.003335 0.003297 0.003335 0.003324 0.000000 0.006407 0.000000 0.000000 0.006989 0.006989 0.006989 0.006989 0.007038 0.007001 0.003444 0.003444 0.003444 0.092254 0.102135 0.108127

54 Cathedral4 Digitate 0.000000 0.000000 0.001625 0.003517 0.000000 0 .000000 0.000000 0.000000 0.001741 0.000000 0 .000000 0.000000 0.003427 0.000000 0.000000 0 .001797 0.000000 0.000000 0.000000 0.000000 0 .000000 0.000000 0.000000 0.000000 0.000000 0 .000000 0.000000 0.000000 0.000000 0.000000 0 .000000 0.000000 0.000000 0.003264 0.001703 0. 000000 0.000000 0.000000 0.001625 0.003254 0. 000000 0.000000 0.000000 0.000000 0.000000 0 .000000 0.000000 0.000000 0.001625 0.000000 0 .000000 0.000000 0.001625 0.001625 0.000000 0 .003254 0.001625 0.001625 0.000000 0.000000 0 .000000 0.000000 0.000000 0.000000 0.000000 0 .000000 0.001625 0.001625 0.000000 0.000000 0 .000000 0.000000 0.000000 0.000000 . 0.000000 0 ,000000 0.000000 0.000000 0.000000 0.000000 0 .000000 0.000000 0.000000 0.000000 0.000000 0 .000000 0.000000 0.000000 0.000000 0.000000 0 .003254 0.000000 0.000000 0.003555 0.003555 0.003555 0.003555 0.003580 0.003561 0.000000 0 .000000 0.000000 0.091134 0.101087 0.107124 Cathedral5 Lamellate 0.000000 0.000000 0.001617 0.003499 0.000000 0 .000000 0.000000 0.000000 0.001741 0.000000 0 .000000 0.000000 0.003409 0.000000 0.000000 0 .001788 0.000000 0.000000 0.000000 0.000000 0 .000000 0.000000 0.000000 0.000000 0.000000 0 .000000 0.000000 0.000000 0.000000 0.000000 0 .000000 0.000000 0.000000 0.003264 0.001694 0. 000000 0.000000 0.000000 0.001617 0.003238 0, 000000 0.000000 0.000000 0.000000 0.000000 0 .000000 0.000000 0.000000 0.001617 0.000000 0 .000000 0.000000 0.001617 0.001617 0.000000 0 ,003238 0.001617 0.001617 0.000000 0.000000 0 ,000000 0.000000 0.000000 0.000000 0.000000 0 .000000 0.001617 0.001617 0.000000 0.000000 0 .000000 0.000000 0.000000 0.000000 0.000000 0 .000000 0.000000 0.000000 0.000000 0.000000 0 .000000 0.000000 0.000000 0.000000 0.000000 0 .000000 0.000000 0.000000 0.000000 0.000000 0 .003238 0.000000 0.000000 0.003536 0.003536 0. 003536 0.003536 0.003561 0.003542 0.000000 0 ,000000 0.000000 0.092596 0.102517 0.108533 Cathedral6_Lamellate 0.001794 0.001741 0.003486 0.005310 0.001741 0.001741 0.001741 0.001741 0.000000

55 0.001741 0.001741 0.001807 0.005236 0.001807 0.001811 0.003619 0.001766 0.001741 0.001741 0.001893 0.001760 0.001741 0.001741 0.001778 0.001760 0.001760 0.001741 0.001760 0.001741 0.001744 0.001741 0.001817 0.001744 0.005254 0.003499 0.001741 0.001741 0.001741 0.003486 0.005236 0.001741 0.001741 0.001741 0.001741 0.001744 0.001775 0.001741 0.001741 0.003486 0.001741 0.001741 0.001741 0.003486 0.003486 0.001741 0.005236 0.003486 0.003486 0.001741 0.001741 0.001741 0.001741 0.001741 0.001741 0.001741 0.001741 0.003486 0.003486 0.001741 0.001741 0.001741 0.001741 0.001741 0.001741 0.001741 0.001882 0.000000 0.001741 0.001744 0.001741 0.001741 0.001741 0.001741 0.001741 0.001741 0.001741 0.001741 0.001741 0.001741 0.001999 0.003486 0.003212 0 .0 0 2 2 0 1 0.005367 0.005367 0.005367 0.005367 0.005405 0.005376 0.001766 0.001766 0.001766 0.093814 0.103874 0.109976 Cathedral7_Intermediate 0.000000 0.000000 0.001688 0.003499 0.000000 0.000000 0.000000 0.000000 0.001741 0.000000 0.000000 0.000000 0.003409 0.000000 0.000000 0.001788 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.003409 0.001694 0.000000 0.000000 0.000000 0.001688 0.003380 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.001688 0.000000 0.000000 0.000000 0.001688 0.001688 0.000000 0.003380 0.001688 0.001688 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.001688 0.001688 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.003380 0.000000 0.000000 0.003536 0.003536 0.003536 0.003536 0.003561 0.003542 0.000000 0.000000 0.000000 0.092596 0.102517 0.108533 Cathedral9 Lamellate: 0.000000 0.000000 0.001683 0.1 0.000000 0.000000 0.000000 0.000000 0.001741 0.000000 0.000000 0.000000 0.003409 0.000000 0.000000 0.001788 0.000000 0.000000 0.000000

56 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.003398 0.001694 0.000000 0.000000 0.000000 0.001683 0.003369 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.001683 0.000000 0.000000 0.000000 0.001683 0.001683 0.000000 0.003369 0.001683 0.001683 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.001683 0.001683 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.003369 0.000000 0.000000 0.003536 0.003536 0.003536 0.003536 0.003561 0.003542 0.000000 0.000000 0.000000 0.092596 0.102517 0.108533 DeadPalm2 Lamellate 0.000000 0.000000 0.001788 0.003580 0.000000 0.000000 0.000000 0.000000 0.001807 0.000000 0.000000 0.000000 0.003580 0.000000 0.000000 0.001788 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.003612 0.001794 0.000000 0.000000 0.000000 0.001788 0.003580 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.001788 0.000000 0.000000 0.000000 0.001788 0.001788 0.000000 0.003580 0.001788 0.001788 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.001788 0.001788 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.001788 0.000000 0.000000 0.003580 0.003580 0.003580 0.003580 0.003580 0.003580 0.000000 0.000000 0.000000 0.093039 0.103216 0.111458 DeadPalm09 Intermediate 0.003555 0.003427 0.005119 0.007013 0.003427 0.003409 0.003427 0.003409 0.005236 0.003409 0.003409 0.003580 0.000000 0.003593 0.003606 0.001788 0.003511 0.003409 0.003427 0.003769 0.003462 0.003409 0.003427 0.003499 0.003462 0.003462 0.003409 0.003462 0.003427 0.003421 0.003409 0.003599 0.003433 0.000000 0.005137 0.003409 0.003409 0.003409 0.005119 0.000000 0.003409 0.003409 0.003409 0.003409 0.003415 0.003517 0.003409 0.003409 0.005119 0.003409 0.003409 0.003409 0.005119 0.001703 0.003409 0.000000 0.001703 0.001703 0.003409 0.003409 0.003409 0.003409 0.003409 0.003409 0.003409 0.003409 0.005119 0.001703 0.003409 0.003409 0.003409 0.003409 0.003409 0.003409 0.003409 0.003734 0.004360 0.003409 0.003415 0.003409 0.003409 0.003409 0.003409 0.003409 0.003409 0.003409 0.003409 0.003409 0.003409 0.003979 0.001703 0.003181 0.004380 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.003492 0.003492 0.003492 0.092596 0.104517 0.106522 Point5_Digitate 0.000000 0.000000 0.001794 0.1 0.000000 0.000000 0.000000 0.000000 0.001807 0.000000 0.000000 0.000000 0.003593 0.000000 0.000000 0.001794 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.003612 0.001801 0.000000 0.000000 0.000000 0.001794 0.003593 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.001794 0.000000 0.000000 0.000000 0.001794 0.001794 0.000000 0.003593 0.001794 0.001794 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.001794 0.001794 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.001794 0.000000 0.000000 0.003593 0.003593 0.003593 0.003593 0.003593 0.003593 0.000000 0.000000 0.000000 0.091368 0.101558 0.109811 yl Digitate 0.000000 0.000000 0.001801 o.i0.003606 0.000000 0.000000 0.000000 0.000000 0.001811 0.000000 0.000000 0.000000 0.003606 0.000000 0.000000 0.001817 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.003606 0.001807 0.000000 0.000000 0.000000 0.001801

58 VO mr-- m m m o o o o 0 ,—1 ,—1 0 0 0 0 0 0 0 VOVOO 1** ON •rt r-- •'T 0 00 0 O 00 00 00 00 00 00 00 00 00 00 VO 0 0 0 0 ON 0 000000000000 •'T ON ON m ON 0 00 00 00 O 00 00 00 00 00 00 00 00 00 VO VO 0 0 0 0 (N m 0 m 0 00 00 0 0 0 0 0 0 0 VOVO 00 VO r-- 00 r-- 00 m r-- m O r--r--r-- r-- r--r-- (N r--0 0 0 0 lO 0 0 0 0 0 0 0 0 0 m m m i—t i—i m 1—1 m O 1—1 1—1 1—1 1—1 1—1 1—1 1—1 1—1 1—1 lO 1—1 0 0 0 0 m 1—1 0 i —i 0 0 0 0 0 0 0 0 0 0 0 O ON 0 0 0 O00O0OO000000000O 0 0 0 0 0 O 0 0 0 00000000000OO 0 0 0 O 0 0 O0OO000000000 *—i 0 0 0 0 0 O 0 0 0 0 0 0 0 0 0 0 0 0 0 0 O O 0 000O00O0OO000000000O 00000 O 0 0 0 00m m o o o o 0 0 <—1 <—1 0 <—1 0 0 0 0 0 ON 00 00 00 r-- , — 1 00 00 00 0 O 00 0 00 00 00 00 00 •'T o o o 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1— < 00 00 00 00 ON 1—1 O 00 00 00 00 O 00 0 00 00 00 00 00 00 VO o g o o o 0 0 0 0 0 0 00 00 0 00 0 0 0 0 0 VO VO r-- r-- 00 00 r-- r-- m O r--0 r--r-- r-- *— < r-- O o 10 o o o 0 0 0 0 0 0 0 0 0 0 0 0 m ON 1—1 1—1 1—1 1—1 1—1 1—1 1—1 m O 1—1 0 1—1 1—1 1—1 1—1 (N 1—1 10 o m o o o 0 0 0 0 00000000000O 00 0 0 0 0 0 0000OO000000O0 ON o 0 0 0 0 0 0 0 0 00000000000O0 00 0 0 0 0 000OO000000O0O o 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 O 0 t-- 000000000OO000000O0O § 6 0 0 0 0 0 0 0 0 ON o o o o o o OOO ,—1 O ,—1 O O O O O VO O r-- 00 •'T 00 00 00 00 00 00 0 00 0 00 OO 00 ,—1 00 00 0 0 0 0 0 0 0 0 OOOOOOOOOOOOO ON 00 ON 00 00 ON00 00 00 00 0 00 00 00 r-- OO 00 00 00 00 ° g 0 0 0 0 0 0 0 0 ON OOO O OOOOOVOO r-- r-- r--r-- r-- 0 r-- m *—1 r-- *—1 r-- o 0 0 0 0 0 0 0 0 10 OOOOOOOOO m O 1—1 0 1—1 m (N 1—1 m 1—1 o 0 0 0 0 0 0 0 0 OOOOOOOOOOOOO 000000000 0 0 0 O 0 O 0 0 O 0 0 o 0 0 0 0 0 0 0 0 OOOOOOOOOOOOO 00000000 0 0 0 0 O 0 O 0 0 O 0 0 o 0 0 0 0 0 0 0 0 OOOOOO OOOOO O O 00 000000000000O0O00O00 § 0 0 0 0 0 0 0 0 0 00 o o o o o o 0 0 0 VOOOOOOO ,—1 VO O 0 00 00 0 r-- OO 00 00 00 00 00 00 00 00 m 00 00 0 00 00 0 0 0 •rt 0 0 0 0 0 0 000OOOOOOOOOO 0 00 00 0 00 00 00 00 00 00 00 00 00 VO 00 00 00 00 00 0 0 0 ON 0 0 0 0 0 0 0 0 0 VOOOOOOO 00 VO O r-- 0 00 n o r-- r--r-- r-- r--r-- 00 r-- m r-- 0 0 0 0 0 0 0 0 0 0 0 m OOOOOO m O f—< 1—1 0 i—t i—t i—t i—t i—t i—t i—t i—t i—t i—t i—t m 1—1 1—1 0 0 0 0 0 0 0 0 000OOOOOOOOOO O0 0 0 O 0 0 0 0 0 0 0 0 0 0 0 0 O00 000000000 0 0 0 O O O O OOOOOOO 0 0 0 O 0 0 0 0 0 0 0 0 0 0 0 0 O 0 0 0 0 0 0 0 0 0 0 0 000OOOOOOOOOO O000O00c>000000000O00 0000000 0 0 c>0 00 Oc>0 0 0 0 0 0 0 00 O0 0 c> 0 0 0 0 0 O O O o %O 0 g g & & E E 0.000000 0.000000 0.000000 0.001753 0.001753 0.000000 0.003511 0.001753 0.001753 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.001753 0.001753 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.001753 0.000000 0.000000 0.003548 0.003548 0.003548 0.003548 0.003574 0.003555 0.000000 0.000000 0.000000 0.091134 0.101087 0.107124 5 Lamellate 0.000000 0.000000 0.001562 0.10.003499 0.000000 0.003197 0.000000 0.000000 0.001741 0.000000 0.000000 0.000000 0.003409 0.000000 0.000000 0.001788 0.000000 0.000000 0.000000 0.000000 0.000000 0.006276 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.003264 0.001633 0.001597 0.000000 0.000000 0.001562 0.003127 0.000000 0.000000 0.001562 0.000000 0.000000 0.000000 0.000000 0.006266 0.001562 0.000000 0.000000 0.008174 0.001562 0.001562 0.000000 0.003127 0.001562 0.001562 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.001562 0.001562 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.003127 0.000000 0.000000 0.003403 0.003403 0.003403 0.003403 0.003427 0.003409 0.000000 0.000000 0.000000 0.092254 0.102135 0.108127 10 Digitate 0.000000 0.000000 0.001625 0.1 0.000000 0.000000 0.000000 0.000000 0.001741 0.000000 0.000000 0.000000 0.003427 0.000000 0.000000 0.001797 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.003264 0.001703 0.000000 0.000000 0.000000 0.001625 0.003254 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.001625 0.000000 0.000000 0.000000 0.001625 0.001625 0.000000 0.003254 0.001625 0.001625 0.000000

60 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.001625 0.001625 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.003254 0.000000 0.000000 0.003555 0.003555 0.003555 0.003555 0.003580 0.003561 0.000000 0.000000 0.000000 0.091134 0.101087 0.107124 lei Lamellate 0.000000 0.000000 0.001882 0.0037690 J 0.000000 0.000000 0.000000 0.000000 0.001893 0.000000 0.000000 0.000000 0.003769 0.000000 0.000000 0.001893 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.003769 0.001889 0.000000 0.000000 0.000000 0.001882 0.003769 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.001882 0.000000 0.000000 0.000000 0.001882 0.001882 0.000000 0.003769 0.001882 0.001882 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.001882 0.001882 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.001882 0.000000 0.000000 0.003769 0.003769 0.003769 0.003769 0.003769 0.003769 0.000000 0.000000 0.000000 0.091870 0.098271 0.104727 le2 Lamellate 0.000000 0.000000 0.001641 0.1 0.000000 0.000000 0.000000 0.000000 0.001760 0.000000 0.000000 0.000000 0.003462 0.000000 0.000000 0.001817 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.003286 0.001720 0.000000 0.000000 0.000000 0.001641 0.003286 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.001641 0.000000 0.000000 0.000000 0.001641 0.001641 0.000000 0.003286 0.001641 0.001641 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.001641 0.001641 0.000000

61 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.003286 0.000000 0.000000 0.003593 0.003593 0.003593 0.003593 0.003619 0.003599 0.000000 0.000000 0.000000 0.090165 0.098192 0.106305 Le3 Digitate 0.000000 0.000000 0.007853 0.003499 0.1 0.000000 0.003197 0.000000 0.000000 0.001741 0.000000 0.000000 0.000000 0.003409 0.000000 0.000000 0.001788 0.000000 0.006276 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.003264 0.008215 0.001597 0.006276 0.006276 0.007853 0.009434 0.006276 0.006276 0.007853 0.006276 0.006286 0.006799 0.006276 0.000000 0.007853 0.006276 0.006276 0.000000 0.007853 0.007853 0.006276 0.009434 0.007853 0.007853 0.006276 0.006543 0.006286 0.006276 0.006276 0.006276 0.006276 0.006276 0.007853 0.007853 0.006276 0.006276 0.006276 0.006276 0.006276 0.006276 0.006276 0.007203 0.008362 0.006276 0.006286 0.006276 0.006276 0.006276 0.004862 0.006276 0.006480 0.006543 0.006469 0.006543 0.006522 0.000000 0.009434 0.000000 0.000000 0.010274 0.010274 0.010274 0.010274 0.010345 0.010292 0.003398 0.003398 0.005068 0.092254 0.102135 0.108127 e6 Lamellate 0.000000 0.000000 0.001625 0.003517 0.1 0.000000 0.000000 0.000000 0.000000 0.001741 0.000000 0.000000 0.000000 0.003427 0.000000 0.000000 0.001797 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.003264 0.001703 0.000000 0.000000 0.000000 0.001625 0.003254 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.001625 0.000000 0.000000 0.000000 0.001625 0.001625 0.000000 0.003254 0.001625 0.001625 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.001625 0.001625 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000

62 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.003254 0.000000 0.000000 0.003555 0.003555 0.003555 0.003555 0.003580 0.003561 0.000000 0.000000 0.000000 0.091134 0.101087 0.107124 Pinnacle7 Lamellate 0.000000 0.000000 0.001657 O.i 0.000000 0.000000 0.000000 0.000000 0.001778 0.000000 0.000000 0.000000 0.003499 0.000000 0.000000 0.001837 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.003319 0.001738 0.000000 0.000000 0.000000 0.001657 0.003319 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.001657 0.000000 0.000000 0.000000 0.001657 0.001657 0.000000 0.003319 0.001657 0.001657 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.001657 0.001657 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.003319 0.000000 0.000000 0.003632 0.003632 0.003632 0.003632 0.003659 0.003639 0.000000 0.000000 0.000000 0.091194 0.099318 0.107532 Sar Agapito2 Lamellate 0.000000 0.000000 0.001641 0.003555 0.000000 0.000000 0.000000 0.000000 0.001760 0.000000 0.000000 0.000000 0.003462 0.000000 0.000000 0.001817 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.003286 0.001720 0.000000 0.000000 0.000000 0.001641 0.003286 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.001641 0.000000 0.000000 0.000000 0.001641 0.001641 0.000000 0.003286 0.001641 0.001641 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.001641 0.001641 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000

63 0.000000 0.000000 0.003286 0.000000 0.000000 0.003593 0.003593 0.003593 0.003593 0.003619 0.003599 0.000000 0.000000 0.000000 0.090165 0.098192 0.106305 San_Agapito3 Lamellate 0.000000 0.000000 0.001641 0.003555 0.000000 0.000000 0.000000 0.000000 0.001760 0.000000 0.000000 0.000000 0.003462 0.000000 0.000000 0.001817 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.003286 0.001720 0.000000 0.000000 0.000000 0.001641 0.003286 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.001641 0.000000 0.000000 0.000000 0.001641 0.001641 0.000000 0.003286 0.001641 0.001641 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.001641 0.001641 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.003286 0.000000 0.000000 0.003593 0.003593 0.003593 0.003593 0.003619 0.003599 0.000000 0.000000 0.000000 0.090165 0.098192 0.106305 San Agapito6 Digitate 0.000000 0.000000 0.001594 0.003499 0.000000 0.003197 0.000000 0.000000 0.001741 0.000000 0.000000 0.000000 0.003409 0.000000 0.000000 0.001788 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.003264 0.001669 0.001597 0.000000 0.000000 0.001594 0.003191 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.001594 0.000000 0.000000 0.000000 0.001594 0.001594 0.000000 0.003191 0.001594 0.001594 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.001594 0.001594 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.003191 0.000000 0.000000 0.003480 0.003480 0.003480 0.003480 0.003505 0.003486 0.000000 0.000000 0.000000 0.092254 0.102135 0.108127 San_Agapito7_Lamellate 0.000000 0.000000 0.001641 0.003555 0.000000 0.000000 0.000000 0.000000 0.001760 0.000000 0.000000 0.000000 0.003462 0.000000 0.000000 0.001817 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.003286 0.001720 0.000000 0.000000 0.000000 0.001641 0.003286 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.001641 0.000000 0.000000 0.000000 0.001641 0.001641 0.000000 0.003286 0.001641 0.001641 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.001641 0.001641 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.003286 0.000000 0.000000 0.003593 0.003593 0.003593 0.003593 0.003619 0.003599 0.000000 0.000000 0.000000 0.090165 0.098192 0.106305 San_Agapito8 Lamellate 0.000000 0.000000 0.001625 0.003517 0.000000 0.000000 0.000000 0.000000 0.001741 0.000000 0.000000 0.000000 0.003427 0.000000 0.000000 0.001797 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.003264 0.001703 0.000000 0.000000 0.000000 0.001625 0.003254 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.001625 0.000000 0.000000 0.000000 0.001625 0.001625 0.000000 0.003254 0.001625 0.001625 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.001625 0.001625 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.003254 0.000000 0.000000 0.003555 0.003555 0.003555 0.003555 0.003580 0.003561 0.000000 0.000000 0.000000 0.091134 0.101087 0.107124 Sombrero9_Intermediate 0.000000 0.000000 0.001697 0.003505 0.000000 0.000000 0.000000 0.000000 0.001744 0.000000 0.000000 0.000000 0.003421 0.000000 0.000000 0.001791 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.003427 0.001703 0.000000 0.000000 0.000000 0.001697 0.003398 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.001697 0.000000 0.000000 0.000000 0.001697 0.001697 0.000000 0.003398 0.001697 0.001697 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.001697 0.001697 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.003398 0.000000 0.000000 0.003542 0.003542 0.003542 0.003542 0.003567 0.003548 0.000000 0.000000 0.000000 0.092941 0.102901 0.108941 Sombrero 10 .amellate 0.000000 0.000000 0.001688 0.003499 0.000000 0.000000 0.000000 0.000000 0.001741 0.000000 0.000000 0.000000 0.003409 0.000000 0.000000 0.001788 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.003409 0.001694 0.000000 0.000000 0.000000 0.001688 0.003380 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.001688 0.000000 0.000000 0.000000 0.001688 0.001688 0.000000 0.003380 0.001688 0.001688 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.001688 0.001688 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.003380 0.000000 0.000000 0.003536 0.003536 0.003536 0.003536 0.003561 0.003542 0.000000 0.000000 0.000000 0.092596 0.102517 0.108533 Beatrice9 Intermediate 0.000000 0.000000 0.000000 0.001797 0.000000 0.000000 0.000000 0.000000 w n M • & O13 r® o o p ooooooopppooooopoppp p o o o o o o o oooppoppooo EL o ooo 1 ooooooooo o o o o o o o o o o o to I—» o o o o o o o o o o o o o o o o o o o o o o o o L"1 ooooooooooo o o1— o ‘ o o o o o 1 »—*o o o o o o o o o o o o o o o o o o o o UJ u> U> 03 ' j o u> o o o o o o o o o o u> o o o o o o I u> u> o o o o o o o o o o u> o o o o Os -fc- 5 u> o oooooooooo to 'J o o o o o o 'J o o o o o o o 'J o o o Os o o o o 00 o o 10 ra to o Os oooooooooo ooooooo SO SO o o o o o o o SO o o o u> o o o o I—* Os SO —i 2 . Os o o o o o o o o o o o SO Os o o o o o o u> SO SO o o o o o o o ' J o o o to o o o ' J r® t®a &f® o p p o o o o o ooooooooooooooo poooo ooooooooooooooo o o o o o o ooooooooooooooooo ooooooooo o o o o o o o o o o o o o ooooooooooooo o oooooo oooooooooo o o o o o o o o o u> u> o u> u> o o o o o o u> o o o o o o o o o to o u> o o o o o o o o o o u> o o o o o 00 UJ to o to o o o o o o to o o o o o o o 00 oo o oooooooo o o 00 o o o o o o SO Os o o Os oooooo ooooooooo oo SO o o o o o o o o o o SO o o o o o o - 00 p o I—* SO o o o o o o SO o o o o o o o I—* o o P o SO o o o o o o o o o o SO -Pa- o o o o o o o o u> o o 00 o o o o Os oooooooooooooo o o o o o o o oooooooooooo o o o o o o p o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o ooooooooooooooo oooo ooooooooooooooo UJ u> u> o u> o o o o o 1—» o 1—» oooooooooo o u> 1—» o o o o o o u> o o o o o o o 1—» o Os to o o o o o o Os o Os oooooooooo o 'Jo ooooo ooooooo'Jo o\ to Os o Os o o o o o to o to oooooooooo o SO SO o o o o o o SO o o o o o o o SO o 'J SO to o o o o o o 00 o 00 oooooooooo o SO ' J o o o o o o SO o o o o o o o ' J o o o o u> o to o Os o o o o oooooooooooooooooooo o ooooooooooo o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o SO o o o oooooooooooooooo oooooo ooooooooooooo UJ o u> u> o o o o o 1—» o t—L 1—» o o o o o o o u> o o u> o o o o o o o t—» o o o o o o o o o to o to u> o o o o o Os o Os Os ooooooo o o oo o o o o o'Jooooooooo o \ o o \ o 00 o o o o o to o to to o o o o o o o u> o o SO o o o o o o o SO o o o o o o o o o o -p* -fc- Os o o o o o 00 o 00 00 o o o o o o o u> o o SO o o o o o o o 'J o o o o o o o o o o o o o - f c - SO Os o to o o o to oooooooooooooooooooo 00 o o o p o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o ooooooooooo o o o o o o o o SO o o o oooooooooooooooo SO o o o o oooooooooooooo u> u> SO u> u> o o o o o o o 1—» 1—» o 1—» u> o o o o 1—‘ u> u> o o o o o ►—* o >—* o o o o o o o o u> to Os to to o o o o o o o Os Os o Os to o o o o ' J o o o o o -J o'Joooooooo Os 'J Os o \ o o o o o o o to to o to ' J o o o o ' J SO o o o o o SO o SO o o o o o o o o SO to to 'J o o o o o o o 00 00 o 00 o o o o o u> SO o o o o o 'J o'Joooooooo SO 'Jo u>o o 'J to Os u> 0.003769 0.003286 0.003264 0.003264 0.003319 0.003286 0.003286 0.003264 0.003286 0.003264 0.003427 0.003409 0.003632 0.003270 0.000000 0.005137 0.003264 0.003264 0.003264 0.004902 0.000000 0.003264 0.003264 0.003264 0.003264 0.003270 0.003548 0.003264 0.003264 0.004902 0.003264 0.003264 0.003409 0.004902 0.001630 0.003264 0.000000 0.001630 0.001630 0.003264 0.003409 0.003264 0.003264 0.003264 0.003264 0.003264 0.003264 0.004902 0.001630 0.003264 0.003264 0.003264 0.003264 0.003264 0.003264 0.003264 0.003769 0.004409 0.003264 0.003270 0.003264 0.003264 0.003264 0.003375 0.003264 0.003375 0.003409 0.003369 0.003409 0.003398 0.003979 0.003264 0.003181 0.004380 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.003523 0.003523 0.003523 0.089492 0.103483 0.105503 erol Lamellate 0.001781 0.001703 0.003264 0.005272 0.1 0.001711 0.005025 0.001703 0.001694 0.003499 0.001694 0.001694 0.001794 0.005137 0.001801 0.001807 0.003593 0.001760 0.001633 0.001703 0.001889 0.001720 0.008215 0.001703 0.001738 0.001720 0.001720 0.001669 0.001720 0.001703 0.001703 0.001694 0.001804 0.001706 0.005137 0.000000 0.003346 0.001630 0.001630 0.003264 0.004902 0.001630 0.001630 0.003270 0.001630 0.001633 0.001694 0.001630 0.008188 0.003264 0.001630 0.001630 0.009836 0.003264 0.003264 0.001630 0.004902 0.003264 0.003264 0.001630 0.001630 0.001633 0.001630 0.001630 0.001630 0.001630 0.001630 0.003264 0.003264 0.001630 0.001630 0.001630 0.001630 0.001630 0.001630 0.001630 0.001794 0.002082 0.001630 0.001633 0.001630 0.001630 0.001630 0.001633 0.001630 0.001630 0.001630 0.001630 0.001630 0.001630 0.001995 0.004902 0.000000 0.002191 0.005119 0.005119 0.005119 0.005119 0.005155 0.005128 0.001703 0.001703 0.001691 0.094570 0.104517 0.110549 Point4 Smoothi 0.000000 0.000000 0.003197 0.10.003499 0.000000 0.000000 0.000000 0.000000 0.001741 0.000000 0.000000 0.000000 0.003409 0.000000 0.000000 0.001788 0.000000 0.001597 0.000000 0.000000 0.000000 0.001597 0.000000 0.000000 0.000000 0.000000 0.001597 0.000000 0.000000

68 0.000000 0.000000 0.000000 0.000000 0.003264 0.003346 0.000000 0.001597 0.001597 0.003197 0.004800 0.001597 0.001597 0.001597 0.001597 0.001599 0.001732 0.001597 0.001597 0.003197 0.001597 0.001597 0.001666 0.003197 0.003197 0.001597 0.004800 0.003197 0.003197 0.001597 0.001666 0.001599 0.001597 0.001597 0.001597 0.001597 0.001597 0.003197 0.003197 0.001597 0.001597 0.001597 0.001597 0.001597 0.001597 0.001597 0.001837 0.002140 0.001597 0.001599 0.001597 0.001597 0.001597 0.001649 0.001597 0.001649 0.001666 0.001647 0.001666 0.001660 0.000000 0.004800 0.000000 0.000000 0.005236 0.005236 0.005236 0.005236 0.005272 0.005245 0.001720 0.001720 0.001720 0.092254 0.102135 0.108127 el Lamellate 0.000000 0.000000 0.001531 0J 0.000000 0.003197 0.000000 0.000000 0.001741 0.000000 0.000000 0.000000 0.003409 0.000000 0.000000 0.001788 0.000000 0.000000 0.000000 0.000000 0.000000 0.006276 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.003264 0.001630 0.001597 0.000000 0.000000 0.001521 0.003046 0.000000 0.000000 0.001524 0.000000 0.000000 0.000000 0.000000 0.006104 0.001521 0.000000 0.000000 0.017610 0.001521 0.001521 0.000000 0.003046 0.001521 0.001521 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.001521 0.001521 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.003046 0.000000 0.000000 0.003308 0.003308 0.003308 0.003308 0.003330 0.003313 0.000000 0.000000 0.000000 0.092254 0.102135 0.108127 e2 P'gitate 0.000000 0.000000 0.001531 0J0.003499 0.000000 0.003197 0.000000 0.000000 0.001741 0.000000 0.000000 0.000000 0.003409 0.000000 0.000000 0.001788 0.000000 0.000000 0.000000 0.000000 0.000000 0.006276 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.003264 0.001630 0.001597 0.000000 0.000000 0.001521

69 0.003046 0.000000 0.000000 0.001524 0.000000 0.000000 0.000000 0.000000 0.006104 0.001521 0.000000 0.000000 0.017610 0.001521 0.001521 0.000000 0.003046 0.001521 0.001521 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.001521 0.001521 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.003046 0.000000 0.000000 0.003308 0.003308 0.003308 0.003308 0.003330 0.003313 0.000000 0.000000 0.000000 0.092254 0.102135 0.108127 e4 Lamellate 0.001775 0.001625 0.000000 0.10.001747 0.001706 0.004800 0.001625 0.001617 0.003486 0.001688 0.001683 0.001788 0.005119 0.001794 0.001801 0.003580 0.001753 0.001562 0.001625 0.001882 0.001641 0.007853 0.001625 0.001657 0.001641 0.001641 0.001594 0.001641 0.001625 0.001697 0.001688 0.000000 0.001628 0.004902 0.003264 0.003197 0.001521 0.001521 0.000000 0.004573 0.001521 0.001521 0.003050 0.001521 0.001524 0.001644 0.001521 0.007638 0.000000 0.001521 0.001521 0.019232 0.000000 0.003046 0.001521 0.004573 0.003046 0.003046 0.001521 0.001584 0.001524 0.001521 0.001521 0.001521 0.001521 0.001521 0.000000 0.003046 0.001521 0.001521 0.001521 0.001521 0.001521 0.001521 0.001521 0.000000 0.000000 0.001521 0.001524 0.001521 0.001521 0.001521 0.001574 0.001521 0.001569 0.001584 0.001567 0.001584 0.001579 0.001987 0.004573 0.003181 0.002187 0.004967 0.004967 0.004967 0.004967 0.005000 0.004975 0.001697 0.001697 0.001685 0.092254 0.100149 0.108127 e6 Lamellate 0.003555 0.003254 0.004601 0.007013 O.i 0.003415 0.006407 0.003254 0.003238 0.005236 0.003380 0.003369 0.003580 0.000000 0.003593 0.003606 0.001788 0.003511 0.003127 0.003254 0.003769 0.003286 0.009434 0.003254 0.003319 0.003286 0.003286 0.003191 0.003286 0.003254 0.003398 0.003380 0.003599 0.003259 0.000000 0.004902 0.004800 0.003046 0.003046 0.004573 0.000000 0.003046 0.003046 0.004580 0.003046 0.003050 0.003291 0.003046 0.009174 0.004573

70 0.003046 0.003046 0.020857 0.004573 0.001521 0.003046 0.000000 0.001521 0.001521 0.003046 0.003171 0.003050 0.003046 0.003046 0.003046 0.003046 0.003046 0.004573 0.001521 0.003046 0.003046 0.003046 0.003046 0.003046 0.003046 0.003046 0.003480 0.004019 0.003046 0.003050 0.003046 0.003046 0.003046 0.003151 0.003046 0.003141 0.003171 0.003136 0.003171 0.003161 0.003979 0.003046 0.003181 0.004380 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.003398 0.003398 0.003375 0.092254 0.106125 0.108127 e7 Lamellate 0.000000 0.000000 0.001531 0.1 0.000000 0.003197 0.000000 0.000000 0.001741 0.000000 0.000000 0.000000 0.003409 0.000000 0.000000 0.001788 0.000000 0.000000 0.000000 0.000000 0.000000 0.006276 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.003264 0.001630 0.001597 0.000000 0.000000 0.001521 0.003046 0.000000 0.000000 0.001524 0.000000 0.000000 0.000000 0.000000 0.006104 0.001521 0.000000 0.000000 0.017610 0.001521 0.001521 0.000000 0.003046 0.001521 0.001521 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.001521 0.001521 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.003046 0.000000 0.000000 0.003308 0.003308 0.003308 0.003308 0.003330 0.003313 0.000000 0.000000 0.000000 0.092254 0.102135 0.108127 e8 Lamellate 0.000000 0.000000 0.001531 0.1 0.000000 0.003197 0.000000 0.000000 0.001741 0.000000 0.000000 0.000000 0.003409 0.000000 0.000000 0.001788 0.000000 0.000000 0.000000 0.000000 0.000000 0.006276 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.003264 0.001630 0.001597 0.000000 0.000000 0.001521 0.003046 0.000000 0.000000 0.001524 0.000000 0.000000 0.000000 0.000000 0.006104 0.001521 0.000000 0.000000 0.017610 0.001521 0.001521 0.000000 0.003046 0.001521 0.001521 0.000000

71 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.001521 0.001521 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.003046 0.000000 0.000000 0.003308 0.003308 0.003308 0.003308 0.003330 0.003313 0.000000 0.000000 0.000000 0.092254 0.102135 0.108127 Beatrice 10 Lamellate 0.000000 0.000000 0.003069 0J 0.000000 0.003197 0.000000 0.000000 0.001741 0.000000 0.000000 0.000000 0.003409 0.000000 0.000000 0.001788 0.000000 0.001562 0.000000 0.000000 0.000000 0.007853 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.003264 0.003270 0.001597 0.001524 0.001524 0.003050 0.004580 0.001524 0.001524 0.000000 0.001524 0.001526 0.001647 0.001524 0.007649 0.003050 0.001524 0.001524 0.019263 0.003050 0.003050 0.001524 0.004580 0.003050 0.003050 0.001524 0.001586 0.001526 0.001524 0.001524 0.001524 0.001524 0.001524 0.003050 0.003050 0.001524 0.001524 0.001524 0.001524 0.001524 0.001524 0.001524 0.001741 0 .0 0 2 0 11 0.001524 0.001526 0.001524 0.001524 0.001524 0.001576 0.001524 0.001572 0.001586 0.001569 0.001586 0.001581 0.000000 0.004580 0.000000 0.000000 0.004975 0.004975 0.004975 0.004975 0.005008 0.004983 0.000000 0.000000 0.001685 0.092254 0.102135 0.108127 Bethlehem2 Lamellate 0.000000 0.000000 0.001531 0.003499 0.000000 0.003197 0.000000 0.000000 0.001741 0.000000 0.000000 0.000000 0.003409 0.000000 0.000000 0.001788 0.000000 0.000000 0.000000 0.000000 0.000000 0.006276 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.003264 0.001630 0.001597 0.000000 0.000000 0.001521 0.003046 0.000000 0.000000 0.001524 0.000000 0.000000 0.000000 0.000000 0.006104 0.001521 0.000000 0.000000 0.017610 0.001521 0.001521 0.000000 0.003046 0.001521 0.001521 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.001521 0.001521

72 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.003046 0.000000 0.000000 0.003308 0.003308 0.003308 0.003308 0.003330 0.003313 0.000000 0.000000 0.000000 0.092254 0.102135 0.108127 iem3 Digitate 0.000000 0.000000 0.001533 00.003505j 0.000000 0.003202 0.000000 0.000000 0.001744 0.000000 0.000000 0.000000 0.003415 0.000000 0.000000 0.001791 0.000000 0.000000 0.000000 0.000000 0.000000 0.006286 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.003270 0.001633 0.001599 0.000000 0.000000 0.001524 0.003050 0.000000 0.000000 0.001526 0.000000 0.000000 0.000000 0.000000 0.006113 0.001524 0.000000 0.000000 0.017638 0.001524 0.001524 0.000000 0.003050 0.001524 0.001524 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.001524 0.001524 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.003050 0.000000 0.000000 0.003313 0.003313 0.003313 0.003313 0.003335 0.003319 0.000000 0.000000 0.000000 0.092424 0.100335 0.106323 iem4 D gitate 0.000000 0.000000 0.001655 0.003517 O.l 0.000000 0.003468 0.000000 0.000000 0.001775 0.000000 0.000000 0.000000 0.003517 0.000000 0.000000 0.001788 0.000000 0.000000 0.000000 0.000000 0.000000 0.006799 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.003548 0.001694 0.001732 0.000000 0.000000 0.001644 0.003291 0.000000 0.000000 0.001647 0.000000 0.000000 0.000000 0.000000 0.006597 0.001644 0.000000 0.000000 0.018283 0.001644 0.001644 0.000000 0.003291 0.001644 0.001644 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.001644 0.001644 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000

73 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.001644 0.000000 0.000000 0.003308 0.003308 0.003308 0.003308 0.003330 0.003313 0.000000 0.000000 0.000000 0.092941 0.102901 0.108941 Bethlehem5 Digitate 0.000000 0.000000 0.001531 0.1 0.000000 0.003197 0.000000 0.000000 0.001741 0.000000 0.000000 0.000000 0.003409 0.000000 0.000000 0.001788 0.000000 0.000000 0.000000 0.000000 0.000000 0.006276 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.003264 0.001630 0.001597 0.000000 0.000000 0.001521 0.003046 0.000000 0.000000 0.001524 0.000000 0.000000 0.000000 0.000000 0.006104 0.001521 0.000000 0.000000 0.017610 0.001521 0.001521 0.000000 0.003046 0.001521 0.001521 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.001521 0.001521 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.003046 0.000000 0.000000 0.003308 0.003308 0.003308 0.003308 0.003330 0.003313 0.000000 0.000000 0.000000 0.092254 0.102135 0.108127 Bethlehem6 Lamellate 0.000000 0.000000 0.007684 0.003499 0.000000 0.003197 0.000000 0.000000 0.001741 0.000000 0.000000 0.000000 0.003409 0.000000 0.000000 0.001788 0.000000 0.006266 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.003264 0.008188 0.001597 0.006104 0.006104 0.007638 0.009174 0.006104 0.006104 0.007649 0.006104 0.006113 0.006597 0.006104 0.000000 0.007638 0.006104 0.006104 0.011159 0.007638 0.007638 0.006104 0.009174 0.007638 0.007638 0.006104 0.006356 0.006113 0.006104 0.006104 0.006104 0.006104 0.006104 0.007638 0.007638 0.006104 0.006104 0.006104 0.006104 0.006104 0.006104 0.006104 0.006977 0.008059 0.006104 0.006113 0.006104 0.006104 0.006104 0.004732 0.006104 0.006296 0.006356 0.006286 0.006356

74 0.006336 0.000000 0.009174 0.000000 0.000000 0.009967 0.009967 0.009967 0.009967 0.010034 0.009984 0.003398 0.003398 0.005068 0.092254 0.102135 0.108127 iem7 Digitate 0.001775 0.001625 0.000000 0J0.001747 0.001706 0.004800 0.001625 0.001617 0.003486 0.001688 0.001683 0.001788 0.005119 0.001794 0.001801 0.003580 0.001753 0.001562 0.001625 0.001882 0.001641 0.007853 0.001625 0.001657 0.001641 0.001641 0.001594 0.001641 0.001625 0.001697 0.001688 0.000000 0.001628 0.004902 0.003264 0.003197 0.001521 0.001521 0.000000 0.004573 0.001521 0.001521 0.003050 0.001521 0.001524 0.001644 0.001521 0.007638 0.000000 0.001521 0.001521 0.019232 0.000000 0.003046 0.001521 0.004573 0.003046 0.003046 0.001521 0.001584 0.001524 0.001521 0.001521 0.001521 0.001521 0.001521 0.000000 0.003046 0.001521 0.001521 0.001521 0.001521 0.001521 0.001521 0.001521 0.000000 0.000000 0.001521 0.001524 0.001521 0.001521 0.001521 0.001574 0.001521 0.001569 0.001584 0.001567 0.001584 0.001579 0.001987 0.004573 0.003181 0.002187 0.004967 0.004967 0.004967 0.004967 0.005000 0.004975 0.001697 0.001697 0.001685 0.092254 0.100149 0.108127 iem8 Digitate 0.000000 0.000000 0.001531 0.1 0.000000 0.003197 0.000000 0.000000 0.001741 0.000000 0.000000 0.000000 0.003409 0.000000 0.000000 0.001788 0.000000 0.000000 0.000000 0.000000 0.000000 0.006276 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.003264 0.001630 0.001597 0.000000 0.000000 0.001521 0.003046 0.000000 0.000000 0.001524 0.000000 0.000000 0.000000 0.000000 0.006104 0.001521 0.000000 0.000000 0.017610 0.001521 0.001521 0.000000 0.003046 0.001521 0.001521 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.001521 0.001521 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.003046 0.000000 0.000000 0.003308 0.003308 0.003308 0.003308 0.003330 0.003313

75 9 L

z z i s o r o S £ I 3 0 r 0 ^ 5 3 3 6 0 * 0 890500*0 86££00’0 8 6 e e o o o 1081300 316130*0 59Z. 1 3 0 * 0 5 9 Z .1 3 0 ’0 59Z. 1 3 0 * 0 S 9 Z .1 3 0 ’0 0 0 0 0 0 0 * 0 OOOOOO’O l s s o z o 'o OOOOOO’O 0 1 9 Z . I 0 0 0 I 9 Z . I 0 0 019Z.I00 0I9Z.I00 0 19Z -10*0 0 I9 Z . 10*0 e w ) 9 i o * o 0I9Z.I00 019Z.100 0 19Z, 10*0 8 £ 9 Z . I 0 0 0 I 9 Z . I 0 0 SL£ZZ O’O 5K610’0 0 I 9 Z - 1 0 0 0 I 9 Z . I 0 0 0 1 9 Z .1 0 0 019Z.I00 0I9Z.I0’0 0 I 9 Z . 1 0 0 0 I 9 Z . I 0 0 3 £ 3 6 1 0 * 0 3£3610*0 0I9Z.I00 0 I9 Z . 10*0 0 I9 Z . lO 'O 0 I 9 Z . 1 0 0 019Z.100 8£9Z.10’0 019Z. 10*0 019Z.100 3£3610*0 3£3610*0 Z.58030’0 0 1 9 Z -10*0 Z£Z610'0 3£3610*0 OOOOOO’O 0 I 9 Z . 1 0 0 0 19Z. 10*0 Z£Z6\0'0 651110*0 0T9Z.I00 £83810*0 8e9Z-I0*0 0 I 9 Z - I 0 0 £ 9 3 6 1 0 * 0 0I9Z-I00 0I9Z-10*0 £58030*0 Z£Z6\0'0 0 19Z, 10*0 0 I 9 Z . I 0 0 9991000 9£8600*0 60K 000 0000000 OOOOOO’O OOOOOO’O OOOOOO’O 0 0 0 0 0 0 0 0000000 OOOOOO’O OOOOOO’O OOOOOO’O 0000000 000000*0 OOOOOO’O OOOOOO’O OOOOOO’O 0000000 W. 1800*0 OOOOOO’O 88Z.I00'0 OOOOOO’O 0000000 60fc£00'0 OOOOOO’O OOOOOO’O OOOOOO’O m i o o o 0000000 OOOOOO’O seeeoo’o OOOOOO’O 10 0Z>W0’0 0000000 0000000 3 1 tn !§ !(3 Z .3 1 8 0 T 0 5 £ i 3 o r o V9ZZ60'0 OOOOOO’O OOOOOO’O OOOOOO’O e i e e o o ’o oeeeoo’o soeeoo’o soeeoo’o soeeooo soeeoo’o OOOOOO’O 0000000 9W)£00*0 OOOOOO’O OOOOOO’O OOOOOO’O 000000*0 OOOOOO’O OOOOOO’O OOOOOO’O OOOOOO’O 0000000 OOOOOO’O OOOOOO’OOOOOOO’O 000000*0 0000000 OOOOOO'O OOOOOO’O OOOOOO’O 0 0 0 0 0 0 * 0 OOOOOO'OOOOOOO’OOOOOOO’OOOOOOO’O 135100*0 135100*0 OOOOOO’O OOOOOO’OOOOOOO’O 000000*0 0000000 OOOOOO’O OOOOOO’OOOOOOO’O 1 3 5 1 0 0 * 0 135100*0 9W)£00*0 OOOOOO’O 135100*0 1 3 5 1 0 0 * 0 0I9Z-10*0 OOOOOO’O OOOOOO’O 135100*0 M ) 1 9 0 0 * 0 0 0 0 0 0 0 0 OOOOOO’OOOOOOO’OOOOOOO’O ^35100*0 OOOOOO’O OOOOOO’O 9W)£00*0 135100*0 0000000 OOOOOO’O Z.65100‘0 0 £ 9 1 0 0 ’0 b9Z£00'0 0000000 OOOOOO’O OOOOOO’O OOOOOO’O OOOOOO’O 0000000 OOOOOO’O OOOOOO’O OOOOOO’O OOOOOO’O OOOOOO’O 9Z.3900*0 OOOOOO’O OOOOOO’O OOOOOO’O 0000000 OOOOOO’O 88AI00’0 OOOOOO’O OOOOOO’O 60t7£00’0 OOOOOO’O OOOOOO’O OOOOOO’OIKIOO’O 0 0 0 0 0 0 0 OOOOOO’O £61 £00*0 OOOOOO’O 66t7£00’0 lesioo'o OOOOOO’O OOOOOO’O 3}T?||3U*PrJ 0 z . z i 8 o r o 5£i3oro *53360*0 OOOOOO’O OOOOOO’O OOOOOO’O DeadPalml_ ,amellate 0.001775 0.001625 0.000000 0.001747 0.001706 0.004800 0.001625 0.001617 0.003486 0.001688 0.001683 0.001788 0.005119 0.001794 0.001801 0.003580 0.001753 0.001562 0.001625 0.001882 0.001641 0.007853 0.001625 0.001657 0.001641 0.001641 0.001594 0.001641 0.001625 0.001697 0.001688 0.000000 0.001628 0.004902 0.003264 0.003197 0.001521 0.001521 0.000000 0.004573 0.001521 0.001521 0.003050 0.001521 0.001524 0.001644 0.001521 0.007638 0.000000 0.001521 0.001521 0.019232 0.000000 0.003046 0.001521 0.004573 0.003046 0.003046 0.001521 0.001584 0.001524 0.001521 0.001521 0.001521 0.001521 0.001521 0.000000 0.003046 0.001521 0.001521 0.001521 0.001521 0.001521 0.001521 0.001521 0.000000 0.000000 0.001521 0.001524 0.001521 0.001521 0.001521 0.001574 0.001521 0.001569 0.001584 0.001567 0.001584 0.001579 0.001987 0.004573 0.003181 0.002187 0.004967 0.004967 0.004967 0.004967 0.005000 0.004975 0.001697 0.001697 0.001685 0.092254 0.100149 0.108127 DeadPalm: Lamellate 0.001775 0.001625 0.003064 0.005254 0.001706 0.004800 0.001625 0.001617 0.003486 0.001688 0.001683 0.001788 0.001703 0.001794 0.001801 0.000000 0.001753 0.001562 0.001625 0.001882 0.001641 0.007853 0.001625 0.001657 0.001641 0.001641 0.001594 0.001641 0.001625 0.001697 0.001688 0.001797 0.001628 0.001630 0.003264 0.003197 0.001521 0.001521 0.003046 0.001521 0.001521 0.001521 0.003050 0.001521 0.001524 0.001644 0.001521 0.007638 0.003046 0.001521 0.001521 0.019232 0.003046 0.000000 0.001521 0.001521 0.000000 0.000000 0.001521 0.001584 0.001524 0.001521 0.001521 0.001521 0.001521 0.001521 0.003046 0.000000 0.001521 0.001521 0.001521 0.001521 0.001521 0.001521 0.001521 0.001738 0.002007 0.001521 0.001524 0.001521 0.001521 0.001521 0.001574 0.001521 0.001569 0.001584 0.001567 0.001584 0.001579 0.001987 0.004573 0.003181 0.002187 0.001652 0.001652 0.001652 0.001652 0.001663 0.001655 0.001697 0.001697 0.001685 0.094220 0.104127 0.110135 DeadPalm4 Lamellate 0.000000 0.000000 0.001531 0.003499 0.000000 0.003197 0.000000 0.000000 0.001741 0.000000 0.000000 0.000000 0.003409 0.000000 0.000000 0.001788 0.000000 0.000000 0.000000 0.000000 0.000000 0.006276 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.003264 0.001630 0.001597 0.000000 0.000000 0.001521 0.003046 0.000000 0.000000 0.001524 0.000000 0.000000 0.000000 0.000000 0.006104 0.001521 0.000000 0.000000 0.017610 0.001521 0.001521 0.000000 0.003046 0.001521 0.001521 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.001521 0.001521 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.003046 0.000000 0.000000 0.003308 0.003308 0.003308 0.003308 0.003330 0.003313 0.000000 0.000000 0.000000 0.092254 0.102135 0.108127 DeadPalm5_Intermediate 0.003555 0.003254 0.004601 0.007013 0.003415 0.006407 0.003254 0.003238 0.005236 0.003380 0.003369 0.003580 0.000000 0.003593 0.003606 0.001788 0.003511 0.003127 0.003254 0.003769 0.003286 0.009434 0.003254 0.003319 0.003286 0.003286 0.003191 0.003286 0.003254 0.003398 0.003380 0.003599 0.003259 0.000000 0.004902 0.004800 0.003046 0.003046 0.004573 0.000000 0.003046 0.003046 0.004580 0.003046 0.003050 0.003291 0.003046 0.009174 0.004573 0.003046 0.003046 0.020857 0.004573 0.001521 0.003046 0.000000 0.001521 0.001521 0.003046 0.003171 0.003050 0.003046 0.003046 0.003046 0.003046 0.003046 0.004573 0.001521 0.003046 0.003046 0.003046 0.003046 0.003046 0.003046 0.003046 0.003480 0.004019 0.003046 0.003050 0.003046 0.003046 0.003046 0.003151 0.003046 0.003141 0.003171 0.003136 0.003171 0.003161 0.003979 0.003046 0.003181 0.004380 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.003398 0.003398 0.003375 0.092254 0.106125 0.108127 DeadPalm6 Digitate 0.001775 0.001625 0.003064 0.1 0.001706 0.004800 0.001625 0.001617 0.003486 0.001688 0.001683 0.001788 0.001703 0.001794 0.001801 0.000000 0.001753 0.001562 0.001625

78 0.001882 0.001641 0.007853 0.001625 0.001657 0.001641 0.001641 0.001594 0.001641 0.001625 0.001697 0.001688 0.001797 0.001628 0.001630 0.003264 0.003197 0.001521 0.001521 0.003046 0.001521 0.001521 0.001521 0.003050 0.001521 0.001524 0.001644 0.001521 0.007638 0.003046 0.001521 0.001521 0.019232 0.003046 0.000000 0.001521 0.001521 0.000000 0.000000 0.001521 0.001584 0.001524 0.001521 0.001521 0.001521 0.001521 0.001521 0.003046 0.000000 0.001521 0.001521 0.001521 0.001521 0.001521 0.001521 0.001521 0.001738 0.002007 0.001521 0.001524 0.001521 0.001521 0.001521 0.001574 0.001521 0.001569 0.001584 0.001567 0.001584 0.001579 0.001987 0.004573 0.003181 0.002187 0.001652 0.001652 0.001652 0.001652 0.001663 0.001655 0.001697 0.001697 0.001685 0.094220 0.104127 0.110135 DeadPalm7_Digitate 0.001775 0.001625 0.003064 0.005254 0.001706 0.004800 0.001625 0.001617 0.003486 0.001688 0.001683 0.001788 0.001703 0.001794 0.001801 0.000000 0.001753 0.001562 0.001625 0.001882 0.00 641 0.007853 0.001625 0.001657 0.001641 0.001641 0.001594 0.001641 0.001625 0.001697 0.001688 0.001797 0.001628 0.001630 0.003264 0.003197 0.001521 0.001521 0.003046 0.001521 0.001521 0.001521 0.003050 0.001521 0.001524 0.001644 0.001521 0.007638 0.003046 0.001521 0.001521 0.019232 0.003046 0.000000 0.001521 0.001521 0.000000 0.000000 0.001521 0.001584 0.001524 0.001521 0.001521 0.001521 0.001521 0.001521 0.003046 0.000000 0.001521 0.001521 0.001521 0.001521 0.001521 0.001521 0.001521 0.001738 0.002007 0.001521 0.001524 0.001521 0.001521 0.001521 0.001574 0.001521 0.001569 0.001584 0.001567 0.001584 0.001579 0.001987 0.004573 0.003181 0.002187 0.001652 0.001652 0.001652 0.001652 0.001663 0.001655 0.001697 0.001697 0.001685 0.094220 0.104127 0.110135 DeadPalm8_Digitate 0.000000 0.000000 0.001531 0.003499 0.000000 0.003197 0.000000 0.000000 0.001741 * 0.000000 0.000000 0.000000 0.003409 0.000000 0.000000 0.001788 0.000000 0.000000 0.000000 0.000000 0.000000 0.006276 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000

79 0.000000 0.000000 0.000000 0.000000 0.003264 0.001630 0.001597 0.000000 0.000000 0.001521 0.003046 0.000000 0.000000 0.001524 0.000000 0.000000 0.000000 0.000000 0.006104 0.001521 0.000000 0.000000 0.017610 0.001521 0.001521 0.000000 0.003046 0.001521 0.001521 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.001521 0.001521 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.003046 0.000000 0.000000 0.003308 0.003308 0.003308 0.003308 0.003330 0.003313 0.000000 0.000000 0.000000 0.092254 0.102135 0.108127 DeadPalm 1 ODigitate 0.000000 0.000000 0.001594 0.1 0.000000 0.003335 0.000000 0.000000 0.001741 0.000000 0.000000 0.000000 0.003409 0.000000 0.000000 0.001788 0.000000 0.000000 0.000000 0.000000 0.000000 0.006543 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.003409 0.001630 0.001666 0.000000 0.000000 0.001584 0.003171 0.000000 0.000000 0.001586 0.000000 0.000000 0.000000 0.000000 0.006356 0.001584 0.000000 0.000000 0.017610 0.001584 0.001584 0.000000 0.003171 0.001584 0.001584 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.001584 0.001584 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.003171 0.000000 0.000000 0.003308 0.003308 0.003308 0.003308 0.003330 0.003313 0.000000 0.000000 0.000000 0.092254 0.102135 0.108127 DevilsPointl Lamellate 0.000000 0.000000 0.001533 0.003499 0.000000 0.001599 0.000000 0.000000 0.001741 0.000000 0.000000 0.000000 0.003409 0.000000 0.000000 0.001788 0.000000 0.000000 0.000000 0.000000 0.000000 0.006286 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.003264 0.001633 0.001599 0.000000 0.000000

80 0.001524 0.003050 0.000000 0.000000 0.001526 0.000000 0.000000 0.000000 0.000000 0.006113 0.001524 0.000000 0.000000 0.017638 0.001524 0.001524 0.000000 0.003050 0.001524 0.001524 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.001524 0.001524 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.003050 0.000000 0.000000 0.003313 0.003313 0.003313 0.003313 0.003335 0.003319 0.000000 0.000000 0.000000 0.092254 0.102135 0.108127 DevilsPoint2_Lamellate 0.000000 0.000000 0.001531 0.003499 0.000000 0.003197 0.000000 0.000000 0.001741 0.000000 0.000000 0.000000 0.003409 0.000000 0.000000 0.001788 0.000000 0.000000 0.000000 0.000000 0.000000 0.006276 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.003264 0.001630 0.001597 0.000000 0.000000 0.001521 0.003046 0.000000 0.000000 0.001524 0.000000 0.000000 0.000000 0.000000 0.006104 0.001521 0.000000 0.000000 0.017610 0.001521 0.001521 0.000000 0.003046 0.001521 0.001521 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.001521 0.001521 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.003046 0.000000 0.000000 0.003308 0.003308 0.003308 0.003308 0.003330 0.003313 0.000000 0.000000 0.000000 0.092254 0.102135 0.108127 DevilsPoint3 Smooth1 0.000000 0.000000 0.001531 0.1 0.000000 0.003197 0.000000 0.000000 0.001741 0.000000 0.000000 0.000000 0.003409 0.000000 0.000000 0.001788 0.000000 0.000000 0.000000 0.000000 0.000000 0.006276 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.003264 0.001630 0.001597 0.000000 0.000000 0.001521 0.003046 0.000000 0.000000 0.001524 0.000000 0.000000 0.000000 0.000000 0.006104 0.001521

81 0.000000 0.000000 0.017610 0.001521 0.001521 0.000000 0.003046 0.001521 0.001521 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.001521 0.001521 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.003046 0.000000 0.000000 0.003308 0.003308 0.003308 0.003308 0.003330 0.003313 0.000000 0.000000 0.000000 0.092254 0.102135 0.108127 DevilsPoint6_Lamellate 0.000000 0.000000 0.001531 0.003499 0.000000 0.003197 0.000000 0.000000 0.001741 0.000000 0.000000 0.000000 0.003409 0.000000 0.000000 0.001788 0.000000 0.000000 0.000000 0.000000 0.000000 0.006276 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.003264 0.001630 0.001597 0.000000 0.000000 0.001521 0.003046 0.000000 0.000000 0.001524 0.000000 0.000000 0.000000 0.000000 0.006104 0.001521 0.000000 0.000000 0.017610 0.001521 0.001521 0.000000 0.003046 0.001521 0.001521 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.001521 0.001521 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.003046 0.000000 0.000000 0.003308 0.003308 0.003308 0.003308 0.003330 0.003313 0.000000 0.000000 0.000000 0.092254 0.102135 0.108127 DevilsPoint7 Intermediate 0.000000 0.000000 0.001531 0.003499 0.000000 0.003197 0.000000 0.000000 0.001741 0.000000 0.000000 0.000000 0.003409 0.000000 0.000000 0.001788 0.000000 0.000000 0.000000 0.000000 0.000000 0.006276 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.003264 0.001630 0.001597 0.000000 0.000000 0.001521 0.003046 0.000000 0.000000 0.001524 0.000000 0.000000 0.000000 0.000000 0.006104 0.001521 0.000000 0.000000 0.017610 0.001521 0.001521 0.000000 0.003046 0.001521 0.001521 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.001521 0.001521 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.003046 0.000000 0.000000 0.003308 0.003308 0.003308 0.003308 0.003330 0.003313 0.000000 0.000000 0.000000 0.092254 0.102135 0.108127 DevilsPoint8_Lamellate 0.000000 0.000000 0.001531 0.003499 0.000000 0.003197 0.000000 0.000000 0.001741 0.000000 0.000000 0.000000 0.003409 0.000000 0.000000 0.001788 0.000000 0.000000 0.000000 0.000000 0.000000 0.006276 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.003264 0.001630 0.001597 0.000000 0.000000 0.001521 0.003046 0.000000 0.000000 0.001524 0.000000 0.000000 0.000000 0.000000 0.006104 0.001521 0.000000 0.000000 0.017610 0.001521 0.001521 0.000000 0.003046 0.001521 0.001521 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.001521 0.001521 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.003046 0.000000 0.000000 0.003308 0.003308 0.003308 0.003308 0.003330 0.003313 0.000000 0.000000 0.000000 0.092254 0.102135 0.108127 ElPinoy2 Lamellate 0.001775 0.001625 0.000000 0.1 0.001706 0.004800 0.001625 0.001617 0.003486 0.001688 0.001683 0.001788 0.005119 0.001794 0.001801 0.003580 0.001753 0.001562 0.001625 0.001882 0.001641 0.007853 0.001625 0.001657 0.001641 0.001641 0.001594 0.001641 0.001625 0.001697 0.001688 0.000000 0.001628 0.004902 0.003264 0.003197 0.001521 0.001521 0.000000 0.004573 0.001521 0.001521 0.003050 0.001521 0.001524 0.001644 0.001521 0.007638 0.000000 0.001521 0.001521 0.019232 0.000000 0.003046 0.001521 0.004573 0.003046 0.003046 0.001521 0.001584 0.001524 0.001521 0.001521 0.001521 0.001521 0.001521 0.000000 0.003046 0.001521

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J 4^ t—* t—* o H-* o Os 00 o 00 H-* L/l to u> o 4^ -£>- H-* H-* o o o o h—» U) LTi o U) O s o o o o o o o o o o o © o o o p o o o o o o o o o o o o o o o o o o o p o o o o o o © © © © © © © © © o o © © © © I—* o o o o o o o o o o o o o o o o o o o H-* o o o o o o o o o o o o o o o o o o o o o o o o o o ooooooooooo oooo ooooooo o o o o o o u> o o o o 4^ H-* H-* o u> H-* u> H-* H-* H-* H-* u> o -£>- 4^ H-* H-* H-* H-* o o o o o L/l L/l o L/l to o o o o - J Os Os L/l L/l L/l L/l L/l L/l L/l o o L/l o Os Os Os Os H-* SO SO L/l L/l L/l L/l o o o o o to to o to Os o o o o 4^ to L/l L/l —j to to to to to to o to u> to L/l to SO 00 /—+s Os —j to to to o o o o o 1—* o 4^ o o o o -J L/l to SO t—* H-* H-* H-* o Os H-* Os o L/l —j L/l Os SO L/l -J SO H-* 4^ u>O L/lo 4^ to SO L/l Os as Os as c*~> © o o o 00 m o o o o o o o o o o o o 00 m o o o o o o o o o o o o o o o m o o o o SO CNo CNCNo o o o o o o o 1— 1 m o o o o so CN o CN CN o o o o o o o o o m m y—1 o o o o CN m o m m o o o o o o o c*~> m *—1 o o o o CN m o m m o o o o o o © o o C*~) c*~> CN 1 o o o o c*~> y—1 o y—1 *—1 o o o o o o o c*~> c*~> CN *—1 o o o o m y—1 o J—1 y—1 o o o o o o © o o © © O o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o p o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o © © o o © o ©©©©©©©©©©©©©©©©©©©© © © © © o o o © © © o © © © o © © min min © o o o o o o o ■"d" (—\ o o o o o o o ■"d" ■"d" ^-1 1 o 1 o o o o o o o o o o o o o ,—1 ^-1 o ^-1 o o o o o o o m m o o o o o o o CN o tN tN o tN o o o o o m m o o o o o o o CN o tN tN o tN o o o o o o o m CN o o o o o o m y—1m m o m o o o o o c*~> tN o o o o o o m y—1 m m o m o o o o o o o c*~> CN o m o o o o o T—l so »—1 »—1 o »—1 o o o o o c*~> tN o c*~> o o o o o T—l so y—( y—( o y—( o o o o o o o © as o ooooooooooooooooooo o o o o o o o o o o o o o o o o o o o o o o o o o o o ooooooooooooooo o o o o o o o o o o o o o o o o o o o o o o o o o oooooooo'oooooooooooo o o o o o o o o o o o o o o o o o o o o o o o o o o o o 00 o ooosooooooo o oo o o 00 o o o o so o o o o o o o o o o o o »—1 in o o o o o oo o t"-o o o o o f—< CN o CN o o o o o o o o o o t" - o o o o o tN o tN o o o o 00 o o o m o o o o CN o o o o o so m o m o o o o o m o o o o CN o o o o o so m o m o o o o o o o c*~> o ooosoooooof" y—( o ^ —1 o o o o o c*~> o o o o so o o o o o f " y— i o y—( o o o o o o o © o o o o o o o o o o T—< o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o oooooooooooo o o o o oooo ooooooo o o o o o o o o o o o o © © © © © o o o o ooooooooooooooooo o o o o o o o o o o o o o o o o o o o o o o o o o o o o so 00 o C-3 ^ o 00 o o o t"-o o o soo o o o o o so 00 c o 00 o o o t"- o o o so o o o o o o o o ■"d" o o ° as o 00 o o o as o o o ■"d" o o o o o o ■"d" o o as o 00 o o o as o o o o o o o o o o o o m o w—( o o o o m o o o o o o o o o o o m o o r^ o o o m o o o o o o o o o o o o m c*~> o m o »—1 o o o ^—1 o o o c*~> o o o o o o m c*~> o m o o o o y—( o o o m o o o o o o ooooo oooooooo oooooooooooo o o o o o o o o o o o o o o o o o o o o o o o o o o oooooooooooooooo o o o o o o o o o o o o o o o o o ooooo ooooooooooooooo o o o o o o o o o o o o o o o o o o o o o o

u. o o o 00 o t"- J 3 o oooooosooooooooooo 00 o t"- J 3 o o o o o o o so o o o o o o o o o oooooCN ooooo m ■"d" ooooooooooooCN ^ ° o o o o o c*~> o o o o o o o o o o o o m o »—1 ooooosoooooooooooo m o f—t 8 o o o o o o so o o o o o o o o o o o o o m o 00 E o o o o o o »—1 c*~> o o o o o o o o o o c*~> o oo E o o o o o o 1—1 m o o o o o o o o o oooooo S oooooooooooooooooo o o o S o o o o o o o o o o o o o o o o o o o o o o >—1 p o o o o o o o o o o o o o o o o o o _) pooooooo o o o o o o p o o o o o o o o o o o o o o o ° oooooooooooooooooooo v c o o o o o o o o o .13 "3 '3 0.000000 0.003046 0.000000 0.000000 0.003308 0.003308 0.003308 0.003308 0.003330 0.003313 0.000000 0.000000 0.000000 0.092254 0.102135 0.108127 Mainit4\ Lamellate 0.000000 0.000000 0.001531 0.1 0.000000 0.003197 0.000000 0.000000 0.001741 0.000000 0.000000 0.000000 0.003409 0.000000 0.000000 0.001788 0.000000 0.000000 0.000000 0.000000 0.000000 0.006276 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.003264 0.001630 0.001597 0.000000 0.000000 0.001521 0.003046 0.000000 0.000000 0.001524 0.000000 0.000000 0.000000 0.000000 0.006104 0.001521 0.000000 0.000000 0.017610 0.001521 0.001521 0.000000 0.003046 0.001521 0.001521 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.001521 0.001521 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.003046 0.000000 0.000000 0.003308 0.003308 0.003308 0.003308 0.003330 0.003313 0.000000 0.000000 0.000000 0.092254 0.102135 0.108127 Mainit5> Intermediate 0.000000 0.000000 0.001531 0.1 0.000000 0.003197 0.000000 0.000000 0.001741 0.000000 0.000000 0.000000 0.003409 0.000000 0.000000 0.001788 0.000000 0.000000 0.000000 0.000000 0.000000 0.006276 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.003264 0.001630 0.001597 0.000000 0.000000 0.001521 0.003046 0.000000 0.000000 0.001524 0.000000 0.000000 0.000000 0.000000 0.006104 0.001521 0.000000 0.000000 0.017610 0.001521 0.001521 0.000000 0.003046 0.001521 0.001521 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.001521 0.001521 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.003046 0.000000 0.000000 0.003308 0.003308 0.003308 0.003308 0.003330 0.003313

86 0.000000 0.000000 0.000000 0.092254 0.102135 0.108127 Mainit63 Lamellate 0.000000 0.000000 0.001531 0.1 0.000000 0.003197 0.000000 0.000000 0.001741 0.000000 0.000000 0.000000 0.003409 0.000000 0.000000 0.001788 0.000000 0.000000 0.000000 0.000000 0.000000 0.006276 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.003264 0.001630 0.001597 0.000000 0.000000 0.001521 0.003046 0.000000 0.000000 0.001524 0.000000 0.000000 0.000000 0.000000 0.006104 0.001521 0.000000 0.000000 0.017610 0.001521 0.001521 0.000000 0.003046 0.001521 0.001521 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.001521 0.001521 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.003046 0.000000 0.000000 0.003308 0.003308 0.003308 0.003308 0.003330 0.003313 0.000000 0.000000 0.000000 0.092254 0.102135 0.108127 Mainit71 Lamellate 0.000000 0.000000 0.001531 O.i oioooooo 0.003197 0.000000 0.000000 0.001741 0.000000 0.000000 0.000000 0.003409 0.000000 0.000000 0.001788 0.000000 0.000000 0.000000 0.000000 0.000000 0.006276 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.003264 0.001630 0.001597 0.000000 0.000000 0.001521 0.003046 0.000000 0.000000 0.001524 0.000000 0.000000 0.000000 0.000000 0.006104 0.001521 0.000000 0.000000 0.017610 0.001521 0.001521 0.000000 0.003046 0.001521 0.001521 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.001521 0.001521 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.003046 0.000000 0.000000 0.003308 0.003308 0.003308 0.003308 0.003330 0.003313 0.000000 0.000000 0.000000 0.092254 0.102135 0.108127

87 Mainit8 Lamellate 0.000000 0.000000 0.000000 0.000000 0.000000 0.003679 0.000000 0.000000 0.001882 0.000000 0.000000 0.000000 0.003734 0.000000 0.000000 0.001865 0.000000 0.000000 0.000000 0.000000 0.000000 0.007203 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.003769 0.001794 0.001837 0.000000 0.000000 0.000000 0.003480 0.000000 0.000000 0.001741 0.000000 0.000000 0.000000 0.000000 0.006977 0.000000 0.000000 0.000000 0.019345 0.000000 0.001738 0.000000 0.003480 0.001738 0.001738 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.001738 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.001738 0.000000 0.000000 0.003480 0.003480 0.003480 0.003480 0.003480 0.003480 0.000000 0.000000 0.000000 0.094801 0.099023 0.113991 ) Intermediate 0.000000 0.000000 0.000000 0.10.000000 0.000000 0.004286 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.004360 0.000000 0.000000 0.002177 0.000000 0.000000 0.000000 0.000000 0.000000 0.008362 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.004409 0.002082 0.002140 0.000000 0.000000 0.000000 0.004019 0.000000 0.000000 0.002011 0.000000 0.000000 0.000000 0.000000 0.008059 0.000000 0.000000 0.000000 0.022375 0.000000 0.002007 0.000000 0.004019 0.002007 0.002007 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.002007 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.002007 0.000000 0.000000 0.004019 0.004019 0.004019 0.004019 0.004019 0.004019 0.000000 0.000000 0.000000 0.099376 0.106826 0.114351 Le4 Lamellate 0.000000 0.000000 0.001531 0j 0.000000 0.003197 0.000000 0.000000 0.001741

88 0.000000 0.000000 0.000000 0.003409 0.000000 0.000000 0.001788 0.000000 0.000000 0.000000 0.000000 0.000000 0.006276 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.003264 0.001630 0.001597 0.000000 0.000000 0.001521 0.003046 0.000000 0.000000 0.001524 0.000000 0.000000 0.000000 0.000000 0.006104 0.001521 0.000000 0.000000 0.017610 0.001521 0.001521 0.000000 0.003046 0.001521 0.001521 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.001521 0.001521 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.003046 0.000000 0.000000 0.003308 0.003308 0.003308 0.003308 0.003330 0.003313 0.000000 0.000000 0.000000 0.092254 0.102135 0.108127 PinnacleS Digitate 0.000000 0.000000 0.001533 0.1 0.000000 0.003202 0.000000 0.000000 0.001744 0.000000 0.000000 0.000000 0.003415 0.000000 0.000000 0.001788 0.000000 0.000000 0.000000 0.000000 0.000000 0.006286 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.003270 0.001633 0.001599 0.000000 0.000000 0.001524 0.003050 0.000000 0.000000 0.001526 0.000000 0.000000 0.000000 0.000000 0.006113 0.001524 0.000000 0.000000 0.017638 0.001524 0.001524 0.000000 0.003050 0.001524 0.001524 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.001524 0.001524. 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.003050 0.000000 0.000000 0.003308 0.003308 0.003308 0.003308 0.003330 0.003313 0.000000 0.000000 0.000000 0.092254 0.102135 0.108127 San Agapito 1 Lamellate 0.000000 0.000000 0.001531 0.003499 0.000000 0.003197 0.000000 0.000000 0.001741 0.000000 0.000000 0.000000 0.003409 0.000000 0.000000 0.001788 0.000000 0.000000

89 o o o o , — 1 , — 1 o o o o o o o , — 1 o OS o o o o o , — 1 o , — 1 o o o o o o »—H o OS o o o o o o o CN o

VO o o o o o o o o o o o o 00 o o o o o VO o o o o o o ,—1 o ,—1 o o o o o 00 o o o o o VO o r - o o o o o y—t CN o CN o o o o o o o o o o o r - o o o o o y—t CN o CN o o o o o o o o o o o r - o CN o o o o o VO m o m o o o o o CO o o o o o CN o o o o o VO m o m o o o o o CO o o o o o CN o VO o o o o o r - ,—1 o ,—1 o o o o o CO o o o o o VO o o o o o r - 1 o ^—1 o o o o o CO o o o o o VO o o o o o o o o o o o o o o o o o o o o o o o o o o o T—( o o o o o o o o o o o o o o o o o o o o o o © o o o o o o o o o o o o o o o o o ooooopooooo o o p o o o o o o © © © © © o ©©©©©©©©©©© ©©©©©©©©©©© © © o o o o o o o o o o o o o o

o o o r - o o o VO o o o o o o VO 00 o o r - o 00 o o o r - o o o VO o o o o o o VO 00 o o r - o 00 o o o o o o OS ooo oooooo oo o OS o 00 o o o OS o o o o o o o o o o o o OS o 00 o o OS o o o m ooooooooooo CO o o 1 o r - ooomoooooooo o o o CO o o T—' o r - o o o o o T—1 o o o CO o o o o o o COCO o o CO o 1 o o o 1 o o o CO o o o o o o COCO o o CO o T—' o o ooooooooooooooooo oooooooooooooooo o o o o o o o o o o o ooooooooooooooooo ooopooooopoooooo o o o o p p o o o o o o o d d o o o o o dddddddd dddddddddd ddddddddddd d d d d d d ooooVOoooooooooo 00 o r- Ooooooovoooooooooo o 00 o r- o o o o o o o o CO ooooooooooo o CN o o o o o o CO o o o o o o o o o o o o CN o o o o o o o o VO ooooooooooo CO o o o o o o o VO o o o o o o o o o o o CO o T—' o o o o o o o o T—1 CO o o o o o o o o o o CO o 00 o o o o o o 1 CO o o o o o o o o o o CO o 00 o o o o o oooooooooooooooooo oooooooooooooo o o o o o o o o o o o o oooooooooooooooop <—1 u oooopooopoooo o o o o p p p »—< u o o o o o d d d d o o o d d d d d o d o d d d cd d d o d d d d d d dddddddddddd cd d d d d d

o o o o o o o o o o o 00 CO m _ OS o o o o o o o o o o o o 00 CO m I_) os o o o o o o VO —i QhQ oooooopoopooooooo o o >—J 0 ,0 o o o o CO dddddddddddd o o o d d d tin o d d d d d d d d d d d d d d d d d d d d S j O o o o o <

C/3 C/3 0.000000 0.000000 0.000000 0.000000 0.000000 0.003264 0.001630 0.001597 0.000000 0.000000 0.001521 0.003046 0.000000 0.000000 0.001524 0.000000 0.000000 0.000000 0.000000 0.006104 0.001521 0.000000 0.000000 0.017610 0.001521 0.001521 0.000000 0.003046 0.001521 0.001521 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.001521 0.001521 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.003046 0.000000 0.000000 0.003308 0.003308 0.003308 0.003308 0.003330 0.003313 0.000000 0.000000 0.000000 0.092254 0.102135 0.108127 ero2_Lamellate 0.000000 0.000000 0.001584 0.10.003499 0.000000 0.003302 0.000000 0.000000 0.001741 0.000000 0.000000 0.000000 0.003409 0.000000 0.000000 0.001788 0.000000 0.000000 0.000000 0.000000 0.000000 0.004862 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.003375 0.001633 0.001649 0.000000 0.000000 0.001574 0.003151 0.000000 0.000000 0.001576 0.000000 0.000000 0.000000 0.000000 0.004732 0.001574 0.000000 0.000000 0.016043 0.001574 0.001574 0.000000 0.003151 0.001574 0.001574 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.001574 0.001574 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.003151 0.000000 0.000000 0.003319 0.003319 0.003319 0.003319 0.003341 0.003324 0.000000 0.000000 0.000000 0.092254 0.102135 0.108127 ero3 .amellate 0.000000 0.000000 0.001531 0.10.003499 0.000000 0.003197 0.000000 0.000000 0.001741 0.000000 0.000000 0.000000 0.003409 0.000000 0.000000 0.001788 0.000000 0.000000 0.000000 0.000000 0.000000 0.006276 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.003264 0.001630 0.001597 0.000000 0.000000 0.001521

91 0.003046 0.000000 0.000000 0.001524 0.000000 0.000000 0.000000 0.000000 0.006104 0.001521 0.000000 0.000000 0.017610 0.001521 0.001521 0.000000 0.003046 0.001521 0.001521 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.001521 0.001521 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.003046 0.000000 0.000000 0.003308 0.003308 0.003308 0.003308 0.003330 0.003313 0.000000 0.000000 0.000000 0.092254 0.102135 0.108127 ero4 ^amellate 0.000000 0.000000 0.001579 0.10.003499 0.000000 0.003302 0.000000 0.000000 0.001741 0.000000 0.000000 0.000000 0.003409 0.000000 0.000000 0.001788 0.000000 0.000000 0.000000 0.000000 0.000000 0.006480 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.003375 0.001630 0.001649 0.000000 0.000000 0.001569 0.003141 0.000000 0.000000 0.001572 0.000000 0.000000 0.000000 0.000000 0.006296 0.001569 0.000000 0.000000 0.017610 0.001569 0.001569 0.000000 0.003141 0.001569 0.001569 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.001569 0.001569 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.003141 0.000000 0.000000 0.003308 0.003308 0.003308 0.003308 0.003330 0.003313 0.000000 0.000000 0.000000 0.092254 0.102135 0.108127 ero5 Lamellate 0.000000 0.000000 0.001594 0.10.003499 0.000000 0.003335 0.000000 0.000000 0.001741 0.000000 0.000000 0.000000 0.003409 0.000000 0.000000 0.001788 0.000000 0.000000 0.000000 0.000000 0.000000 0.006543 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.003409 0.001630 0.001666 0.000000 0.000000 0.001584 0.003171 0.000000 0.000000 0.001586 0.000000 0.000000 0.000000 0.000000 0.006356 0.001584

92 0.000000 0.000000 0.017610 0.001584 0.001584 0.000000 0.003171 0.001584 0.001584 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.001584 0.001584 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.003171 0.000000 0.000000 0.003308 0.003308 0.003308 0.003308 0.003330 0.003313 0.000000 0.000000 0.000000 0.092254 0.102135 0.108127 ero6_ ,amellate 0.000000 0.000000 0.001576 0.003499 0.1 0.000000 0.003297 0.000000 0.000000 0.001741 0.000000 0.000000 0.000000 0.003409 0.000000 0.000000 0.001788 0.000000 0.000000 0.000000 0.000000 0.000000 0.006469 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.003369 0.001630 0.001647 0.000000 0.000000 0.001567 0.003136 0.000000 0.000000 0.001569 0.000000 0.000000 0.000000 0.000000 0.006286 0.001567 0.000000 0.000000 0.017610 0.001567 0.001567 0.000000 0.003136 0.001567 0.001567 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.001567 0.001567 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.003136 0.000000 0.000000 0.003308 0.003308 0.003308 0.003308 0.003330 0.003313 0.000000 0.000000 0.000000 0.092254 0.102135 0.108127 ero7 E .gitate 0.000000 0.000000 0.001594 0.003499 0.1 0.000000 0.003335 0.000000 0.000000 0.001741 0.000000 0.000000 0.000000 0.003409 0.000000 0.000000 0.001788 0.000000 0.000000 0.000000 0.000000 0.000000 0.006543 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.003409 0.001630 0.001666 0.000000 0.000000 0.001584 0.003171 0.000000 0.000000 0.001586 0.000000 0.000000 0.000000 0.000000 0.006356 0.001584 0.000000 0.000000 0.017610 0.001584 0.001584 0.000000 0.003171 0.001584 0.001584 0.000000

93 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.001584 0.001584 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.003171 0.000000 0.000000 0.003308 0.003308 0.003308 0.003308 0.003330 0.003313 0.000000 0.000000 0.000000 0.092254 0.102135 0.108127 Sombrero8_Lamellate 0.000000 0.000000 0.001589 0.1 0.000000 0.003324 0.000000 0.000000 0.001741 0.000000 0.000000 0.000000 0.003409 0.000000 0.000000 0.001788 0.000000 0.000000 0.000000 0.000000 0.000000 0.006522 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.003398 0.001630 0.001660 0.000000 0.000000 0.001579 0.003161 0.000000 0.000000 0.001581 0.000000 0.000000 0.000000 0.000000 0.006336 0.001579 0.000000 0.000000 0.017610 0.001579 0.001579 0.000000 0.003161 0.001579 0.001579 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.001579 0.001579 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.003161 0.000000 0.000000 0.003308 0.003308 0.003308 0.003308 0.003330 0.003313 0.000000 0.000000 0.000000 0.092254 0.102135 0.108127 Xestospongia testudinaria Red Sea 1 0.000000 0.000000 0.001987 0.003979 0.000000 0.000000 0.000000 0.000000 0.001999 0.000000 0.000000 0.000000 0.003979 0.000000 0.000000 0.001987 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.003979 0.001995 0.000000 0.000000 0.000000 0.001987 0.003979 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.001987 0.000000 0.000000 0.000000 0.001987 0.001987 0.000000 0.003979 0.001987 0.001987 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.001987

94 0.001987 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.001987 0.000000 0.000000 0.003979 0.003979 0.003979 0.003979 0.003979 0.003979 0.000000 0.000000 0.000000 0.097319 0.101851 0.113302 Dongiatestudinaria__Myanmar_ 0.001775 0.003254 0.004601 0.005254 0.003415 0.006407 0.003254 0.003238 0.003486 0.003380 0.003369 0.001788 0.001703 0.001794 0.001801 0.003580 0.001753 0.003127 0.003254 0.001882 0.003286 0.009434 0.003254 0.003319 0.003286 0.003286 0.003191 0.003286 0.003254 0.003398 0.003380 0.001797 0.003259 0.003264 0.004902 0.004800 0.003046 0.003046 0.004573 0.003046 0.003046 0.003046 0.004580 0.003046 0.003050 0.001644 0.003046 0.009174 0.004573 0.003046 0.003046 0.020857 0.004573 0.004573 0.003046 0.003046 0.004573 0.004573 0.003046 0.003171 0.003050 0.003046 0.003046 0.003046 0.003046 0.003046 0.004573 0.004573 0.003046 0.003046 0.003046 0.003046 0.003046 0.003046 0.003046 0.001738 0.002007 0.003046 0.003050 0.003046 0.003046 0.003046 0.003151 0.003046 0.003141 0.003171 0.003136 0.003171 0.003161 0.001987 0.000000 0.000000 0.002187 0.001652 0.001652 0.001652 0.001652 0.001663 0.001655 0.001697 0.001697 0.001685 0.090293 0.104127 0.106125 3ongia_testudinaria_ Red Sea 2 0.000000 0.000000 0.003181 0.006376 0.000000 0.000000 0.000000 0.000000 0.003212 0.000000 0.000000 0.000000 0.003181 0.000000 0.000000 0.003181 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.003181 0.000000 0.000000 0.000000 0.000000 0.003181 0.003181 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.003181 0.000000 0.000000 0.000000 0.003181 0.003181 0.000000 0.003181 0.003181 0.003181 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.003181 0.003181 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000

95 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.003181 0.003181 0.003181 0.003181 0.003181 0.003181 0.000000 0.000000 0.000000 0.076819 0.116540 0.120257 3ongia_testudinaria_ Red Sea 3 0.000000 0.000000 0.002187 0.004380 0.000000 0.000000 0.000000 0.000000 0.002201 0.000000 0.000000 0.000000 0.004380 0.000000 0.000000 0.002187 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.004380 0.002191 0.000000 0.000000 0.000000 0.002187 0.004380 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.002187 0.000000 0.000000 0.000000 0.002187 0.002187 0.000000 0.004380 0.002187 0.002187 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.002187 0.002187 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.002187 0.000000 0.000000 0.004380 0.004380 0.004380 0.004380 0.004380 0.004380 0.000000 0.000000 0.000000 0.087928 0.110355 0.110355 Dongia testudinaria Viet Nam l 0.003555 0.003555 0.005000 0.007088 0.003555 0.006989 0.003555 0.003536 0.005367 0.003536 0.003536 0.003580 0.000000 0.003593 0.003606 0.001788 0.003548 0.003403 0.003555 0.003769 0.003593 0.010274 0.003555 0.003632 0.003593 0.003593 0.003480 0.003593 0.003555 0.003542 0.003536 0.003599 0.003561 0.000000 0.005119 0.005236 0.003308 0.003308 0.004967 0.000000 0.003308 0.003308 0.004975 0.003308 0.003313 0.003308 0.003308 0.009967 0.004967 0.003308 0.003308 0.021765 0.004967 0.001652 0.003308 0.000000 0.001652 0.001652 0.003308 0.003308 0.003313 0.003308 0.003308 0.003308 0.003308 0.003308 0.004967 0.001652 0.003308 0.003308 0.003308 0.003308 0.003308 0.003308 0.003308 0.003480 0.004019 0.003308 0.003308 0.003308 0.003308 0.003308 0.003319 0.003308 0.003308 0.003308 0.003308

96 0.003308 0.003308 0.003979 0.001652 0.003181 0.004380 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.003438 0.003438 0.003415 0.091474 0.105503 0.109560 Dongiatestudinarl _Viet_Nam_2 0.003555 0.003555 0.005000 0.007088 0.003555 0.006989 0.003555 0.003536 0.005367 0.003536 0.003536 0.003580 0.000000 0.003593 0.003606 0.001788 0.003548 0.003403 0.003555 0.003769 0.003593 0.010274 0.003555 0.003632 0.003593 0.003593 0.003480 0.003593 0.003555 0.003542 0.003536 0.003599 0.003561 0.000000 0.005119 0.005236 0.003308 0.003308 0.004967 0.000000 0.003308 0.003308 0.004975 0.003308 0.003313 0.003308 0.003308 0.009967 0.004967 0.003308 0.003308 0.021765 0.004967 0.001652 0.003308 0.000000 0.001652 0.001652 0.003308 0.003308 0.003313 0.003308 0.003308 0.003308 0.003308 0.003308 0.004967 0.001652 0.003308 0.003308 0.003308 0.003308 0.003308 0.003308 0.003308 0.003480 0.004019 0.003308 0.003308 0.003308 0.003308 0.003308 0.003319 0.003308 0.003308 0.003308 0.003308 0.003308 0.003308 0.003979 0.001652 0.003181 0.004380 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.003438 0.003438 0.003415 0.091474 0.105503 0.109560 Dongiate studinari a__Viet Nan: 3 0.003555 0.003555 0.005000 0.007088 0.003555 0.006989 0.003555 0.003536 0.005367 0.003536 0.003536 0.003580 0.000000 0.003593 0.003606 0.001788 0.003548 0.003403 0.003555 0.003769 0.003593 0.010274 0.003555 0.003632 0.003593 0.003593 0.003480 0.003593 0.003555 0.003542 0.003536 0.003599 0.003561 0.000000 0.005119 0.005236 0.003308 0.003308 0.004967 0.000000 0.003308 0.003308 0.004975 0.003308 0.003313 0.003308 0.003308 0.009967 0.004967 0.003308 0.003308 0.021765 0.004967 0.001652 0.003308 0.000000 0.001652 0.001652 0.003308 0.003308 0.003313 0.003308 0.003308 0.003308 0.003308 0.003308 0.004967 0.001652 0.003308 0.003308 0.003308 0.003308 0.003308 0.003308 0.003308 0.003480 0.004019 0.003308 0.003308 0.003308 0.003308 0.003308 0.003319 0.003308 0.003308 0.003308 0.003308 0.003308 0.003308 0.003979 0.001652 0.003181 0.004380 0.000000 0.000000 0.000000 0.000000

97 0.000000 0.000000 0.003438 0.003438 0.003415 0.091474 0.105503 0.109560 pongia_testudinaria__V iet_N am_4 0.003555 0.003555 0.005000 0.007088 0.003555 0.006989 0.003555 0.003536 0.005367 0.003536 0.003536 0.003580 0.000000 0.003593 0.003606 0.001788 0.003548 0.003403 0.003555 0.003769 0.003593 0.010274 0.003555 0.003632 0.003593 0.003593 0.003480 0.003593 0.003555 0.003542 0.003536 0.003599 0.003561 0.000000 0.005119 0.005236 0.003308 0.003308 0.004967 0.000000 0.003308 0.003308 0.004975 0.003308 0.003313 0.003308 0.003308 0.009967 0.004967 0.003308 0.003308 0.021765 0.004967 0.001652 0.003308 0.000000 0.001652 0.001652 0.003308 0.003308 0.003313 0.003308 0.003308 0.003308 0.003308 0.003308 0.004967 0.001652 0.003308 0.003308 0.003308 0.003308 0.003308 0.003308 0.003308 0.003480 0.004019 0.003308 0.003308 0.003308 0.003308 0.003308 0.003319 0.003308 0.003308 0.003308 0.003308 0.003308 0.003308 0.003979 0.001652 0.003181 0.004380 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.003438 0.003438 0.003415 0.091474 0.105503 0.109560 Dongiatestudinaria__V iet_ Nam 5 0.003561 0.003580 0.005034 0.007139 0.003580 0.007038 0.003580 0.003561 0.005405 0.003561 0.003561 0.003580 0.000000 0.003593 0.003619 0.001788 0.003574 0.003427 0.003580 0.003769 0.003619 0.010345 0.003580 0.003659 0.003619 0.003619 0.003505 0.003619 0.003580 0.003567 0.003561 0.003599 0.003586 0.000000 0.005155 0.005272 0.003330 0.003330 0.005000 0.000000 0.003330 0.003330 0.005008 0.003330 0.003335 0.003330 0.003330 0.010034 0.005000 0.003330 0.003330 0.021912 0.005000 0.001663 0.003330 0.000000 0.001663 0.001663 0.003330 0.003330 0.003335 0.003330 0.003330 0.003330 0.003330 0.003330 0.005000 0.001663 0.003330 0.003330 0.003330 0.003330 0.003330 0.003330 0.003330 0.003480 0.004019 0.003330 0.003330 0.003330 0.003330 0.003330 0.003341 0.003330 0.003330 0.003330 0.003330 0.003330 0.003330 0.003979 0.001663 0.003181 0.004380 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.003462 0.003462 0.003438 0.092163 0.106305 0.110395

98 Xestospongia testudinaria__Viet_Nam 6 0.003555 0.003561 0.005008 0.007101 0.003561 0.007001 0.003561 0.003542 0.005376 0.003542 0.003542 0.003580 0.000000 0.003593 0.003606 0.001788 0.003555 0.003409 0.003561 0.003769 0.003599 0.010292 0.003561 0.003639 0.003599 0.003599 0.003486 0.003599 0.003561 0.003548 0.003542 0.003599 0.003567 0.000000 0.005128 0.005245 0.003313 0.003313 0.004975 0.000000 0.003313 0.003313 0.004983 0.003313 0.003319 0.003313 0.003313 0.009984 0.004975 0.003313 0.003313 0.021801 0.004975 0.001655 0.003313 0.000000 0.001655 0.001655 0.003313 0.003313 0.003319 0.003313 0.003313 0.003313 0.003313 0.003313 0.004975 0.001655 0.003313 0.003313 0.003313 0.003313 0.003313 0.003313 0.003313 0.003480 0.004019 0.003313 0.003313 0.003313 0.003313 0.003313 0.003324 0.003313 0.003313 0.003313 0.003313 0.003313 0.003313 0.003979 0.001655 0.003181 0.004380 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.003444 0.003444 0.003421 0.091646 0.105702 0.109767 Xestospongia_mutal 0.000000 0.000000 0.001697 0.003499 0.000000 0.003444 0.000000 0.000000 0.001766 0.000000 0.000000 0.000000 0.003492 0.000000 0.000000 0.001788 0.000000 0.000000 0.000000 0.000000 0.000000 0.003398 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.003523 0.001703 0.001720 0.000000 0.000000 0.001697 0.003398 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.003398 0.001697 0.000000 0.000000 0.003398 0.001697 0.001697 0.000000 0.003398 0.001697 0.001697 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.001697 0.001697 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.001697 0.000000 0.000000 0.003438 0.003438 0.003438 0.003438 0.003462 0.003444 0.000000 0.000000 0.000000 0.092254 0.102135 0.108127 Xestospongia_muta2 0.000000 0.000000 0.001697 0.003499 0.000000 0.003444 0.000000 0.000000 0.001766

99 0.000000 0.000000 0.000000 0.003492 0.000000 0.000000 0.001788 0.000000 0.000000 0.000000 0.000000 0.000000 0.003398 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.003523 0.001703 0.001720 0.000000 0.000000 0.001697 0.003398 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.003398 0.001697 0.000000 0.000000 0.003398 0.001697 0.001697 0.000000 0.003398 0.001697 0.001697 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.001697 0.001697 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.001697 0.000000 0.000000 0.003438 0.003438 0.003438 0.003438 0.003462 0.003444 0.000000 0.000000 0.000000 0.092254 0.102135 0.108127 Xestospongia muta3 0.000000 0.000000 0.001685 O.i 0.000000 0.003444 0.000000 0.000000 0.001766 0.000000 0.000000 0.000000 0.003492 0.000000 0.000000 0.001788 0.000000 0.000000 0.000000 0.000000 0.000000 0.005068 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.003523 0.001691 0.001720 0.000000 0.000000 0.001685 0.003375 0.000000 0.000000 0.001685 0.000000 0.000000 0.000000 0.000000 0.005068 0.001685 0.000000 0.000000 0.005068 0.001685 0.001685 0.000000 0.003375 0.001685 0.001685 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.001685 0.001685 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.000000 0.001685 0.000000 0.000000 0.003415 0.003415 0.003415 0.003415 0.003438 0.003421 0.000000 0.000000 0.000000 0.092254 0.102135 0.108127 Petrosia sp.l JN242216 0.092337 0.091134 0.092254 0.094745 0.091134 0.092254 0.091134 0.092596 0.093814 0.092596 0.092596 0.093039 0.092596 0.091368 0.089687 0.095064 0.091134 0.092254

100 0.091134 0.091870 0.090165 0.092254 0.091134 0.091194 0.090165 0.090165 0.092254 0.090165 0.091134 0.092941 0.092596 0.093573 0.091304 0.089492 0.094570 0.092254 0.092254 0.092254 0.092254 0.092254 0.092254 0.092254 0.092254 0.092254 0.092424 0.092941 0.092254 0.092254 0.092254 0.092254 0.092254 0.092254 0.092254 0.094220 0.092254 0.092254 0.094220 0.094220 0.092254 0.092254 0.092254 0.092254 0.092254 0.092254 0.092254 0.092254 0.092254 0.094220 0.092254 0.092254 0.092254 0.092254 0.092254 0.092254 0.092254 0.094801 0.099376 0.092254 0.092254 0.092254 0.092254 0.092254 0.092254 0.092254 0.092254 0.092254 0.092254 0.092254 0.092254 0.097319 0.090293 0.076819 0.087928 0.091474 0.091474 0.091474 0.091474 0.092163 0.091646 0.092254 0.092254 0.092254 0.000000 0.110135 0.130517 a_sp.2_JN242220_ 0.102432 0.101087 0.100149 0.098716 0.101087 0.102135 0.101087 0.102517 0.103874 0.102517 0.102517 0.103216 0.104517 0.101558 0.097840 0.105268 0.101087 0.102135 0.101087 0.098271 0.098192 0.102135 0.101087 0.099318 0.098192 0.098192 0.102135 0.098192 0.101087 0.102901 0.102517 0.101753 0.099272 0.103483 0.104517 0.102135 0.102135 0.102135 0.100149 0.106125 0.102135 0.102135 0.102135 0.102135 0.100335 0.102901 0.102135 0.102135 0.100149 0.102135 0.102135 0.102135 0.100149 0.104127 0.102135 0.106125 0.104127 0.104127 0.102135 0.102135 0.102135 0.102135 0.102135 0.102135 0.102135 0.102135 0.100149 0.104127 0.102135 0.102135 0.102135 0.102135 0.102135 0.102135 0.102135 0.099023 0.106826 0.102135 0.102135 0.102135 0.102135 0.102135 0.102135 0.102135 0.102135 0.102135 0.102135 0.102135 0.102135 0.101851 0.104127 0.116540 0.110355 0.105503 0.105503 0.105503 0.105503 0.106305 0.105702 0.102135 0.102135 0.102135 0.110135 0.000000 0.114168 a sp.3 JN242215 0.110606 0.107124 0.108127 0.110757 0.107124 0.108127 0.107124 0.108533 0.109976 0.108533 0.108533 0.111458 0.106522 0.109811 0.108156 0.113533 0.107124 0.108127 0.107124 0.104727 0.106305 0.108127 0.107124 0.107532 0.106305 0.106305 0.108127 0.106305

101 0.107124 0.108941 0.108533 0.112105 0.107326 0.105503 0.110549 0.108127 0.108127 0.108127 0.108127 0.108127 0.108127 0.108127 0.108127 0.108127 0.106323 0.108941 0.108127 0.108127 0.108127 0.108127 0.108127 0.108127 0.108127 0.110135 0.108127 0.108127 0.110135 0.110135 0.108127 0.108127 0.108127 0.108127 0.108127 0.108127 0.108127 0.108127 0.108127 0.110135 0.108127 0.108127 0.108127 0.108127 0.108127 0.108127 0.108127 0.113991 0.114351 0.108127 0.108127 0.108127 0.108127 0.108127 0.108127 0.108127 0.108127 0.108127 0.108127 0.108127 0.108127 0.113302 0.106125 0.120257 0.110355 0.109560 0.109560 0.109560 0.109560 0.110395 0.109767 0.108127 0.108127 0.108127 0.130517 0.114168 0.000000

102