PPARGC1A antibody (pAb) Rabbit Anti-Human/Mouse/Rat Peroxisome proliferator-activated receptor gamma 1-alpha (PGC1A, LEM6)

Instruction Manual

Catalog Number PK-AB718-7793 Synonyms PPARGC1A Antibody: LEM6, PGC1, PGC1A, PGC-1v, PPARGC1, PGC-1(alpha), LEM6, Peroxisome proliferator-activated receptor gamma coactivator 1-alpha, Ligand effect modulator 6, PGC-1- alpha Description The peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PPARGC1A), also known as LEM-6, is a transcriptional coactivator that regulates the involved in energy (1). PPARGC1A interacts with PPARgamma, which permits the interaction of PPARGC1A with multiple transcription factors. PPARGC1A can interact with, and regulate the activities of, cAMP response element binding (CREB) and nuclear respiratory factors (NRFs). It provides a direct link between external physiological stimuli and the regulation of , and is a major factor that regulates muscle fiber type determination (2). PPARGC1A may be also involved in the development of obesity (3). Quantity 100 µg Source / Host Rabbit Subclass Rabbit IgG Immunogen PPARGC1A antibody was raised against a 17 peptide near the amino terminus of human PPARGC1A. Specific Species React. Human, Mouse, Rat Cross-Reactivity PPARGC1A antibody is human, mouse and rat reactive. At least three isoforms of PPARGC1A are known to exist; this antibody will detect all three isoforms. Formulation Antibody is supplied in PBS containing 0.02% sodium azide. Stabilizer sodium azide Concentration 1 mg/ml Reconstitution During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 ʅl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container͛s cap. Purification Method Affinity chromatography purified via peptide column. Applications PPARGC1A antibody can be used for detection of PPARGC1A by Western blot at 1 - 2 µg/ml. Antibody can also be used for Immunohistochemistry at 5 µg/mL. For Immunoflorescence start at 20 µg/mL. E, WB, IHC, IF |Note: Antibody might be suitable for other applications not tested so far. Optimal concentrations for each application have to be determined individually. Storage Antibody can be stored at 4°C for three months and at -20°C for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. References Tsuemi T and La Spada AR. PGC-1a at the intersection of regulation and neuron function: from Huntington͛s disease to Parkinson͛s disease and beyond. Prog. Neurobiol. 2012; 97:142-51. Kang C and Li Ji L. Role of PGC-1a signaling in health and disease. Ann. NY Acad. Sci. 2012; 1271:110-7.

02/2020 PPARGC1A antibody (pAb) Rabbit Anti-Human/Mouse/Rat Peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC1A, LEM6)

Liu C and Lin JD. PGC-1 coactivators in the control of energy metabolism. Acta Biochim. Biophys. Sin. 2011; 43:248-57. Related Products Cat. No. PK-AB718-7793P - PPARGC1A Peptide Cat. No. PK-AB718-1301 - Human Heart Tissue Lysate

02/2020