The Soil Microbiome Influences Grapevine-Associated Microbiota
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Thymelaeaceae)
Origin and diversification of the Australasian genera Pimelea and Thecanthes (Thymelaeaceae) by MOLEBOHENG CYNTHIA MOTS! Thesis submitted in fulfilment of the requirements for the degree PHILOSOPHIAE DOCTOR in BOTANY in the FACULTY OF SCIENCE at the UNIVERSITY OF JOHANNESBURG Supervisor: Dr Michelle van der Bank Co-supervisors: Dr Barbara L. Rye Dr Vincent Savolainen JUNE 2009 AFFIDAVIT: MASTER'S AND DOCTORAL STUDENTS TO WHOM IT MAY CONCERN This serves to confirm that I Moleboheng_Cynthia Motsi Full Name(s) and Surname ID Number 7808020422084 Student number 920108362 enrolled for the Qualification PhD Faculty _Science Herewith declare that my academic work is in line with the Plagiarism Policy of the University of Johannesburg which I am familiar. I further declare that the work presented in the thesis (minor dissertation/dissertation/thesis) is authentic and original unless clearly indicated otherwise and in such instances full reference to the source is acknowledged and I do not pretend to receive any credit for such acknowledged quotations, and that there is no copyright infringement in my work. I declare that no unethical research practices were used or material gained through dishonesty. I understand that plagiarism is a serious offence and that should I contravene the Plagiarism Policy notwithstanding signing this affidavit, I may be found guilty of a serious criminal offence (perjury) that would amongst other consequences compel the UJ to inform all other tertiary institutions of the offence and to issue a corresponding certificate of reprehensible academic conduct to whomever request such a certificate from the institution. Signed at _Johannesburg on this 31 of _July 2009 Signature Print name Moleboheng_Cynthia Motsi STAMP COMMISSIONER OF OATHS Affidavit certified by a Commissioner of Oaths This affidavit cordons with the requirements of the JUSTICES OF THE PEACE AND COMMISSIONERS OF OATHS ACT 16 OF 1963 and the applicable Regulations published in the GG GNR 1258 of 21 July 1972; GN 903 of 10 July 1998; GN 109 of 2 February 2001 as amended. -
Charles Darwin Reserve
CHARLES DARWIN RESERVE (WHITE WELLS STATION) WESTERN AUSTRALIA FIELD HERBARIUM Volunteers of the Bushland Plant Survey Project Wildflower Society of Western Australia (Inc.) PO Box 519 Floreat WA 6014 for Bush Heritage Australia July 2010 This project was supported by the Wildflower Society of Western Australia Support was also provided by the WA Department of Environment and Conservation NOTE: This Field Herbarium is to remain the property of Bush Heritage, in so long as the Reserve is managed sympathetically with the bushland, and the owners are able to care for the Herbarium so it does not deteriorate. In the event these criteria cannot be met the Field Herbarium is to be handed over to the Geraldton Regional Herbarium. For further information contact the WA Herbarium, Department of Environment and Conservation, Locked Bag 104, Bentley Delivery Centre, WA 6983 Phone (08) 9334 0500. Charles Darwin Reserve (White Wells Station), Western Australia – Field Herbarium CONTENTS 1 BACKGROUND AND ACKNOWLEDGEMENTS..................................................................................... 1 Map 1 Wildflower Society of WA survey sites at Charles Darwin Reserve - August 2008 .......................... 2 Map 2 Wildflower Society of WA survey sites at Charles Darwin Reserve – October 2008 ........................ 3 2 FLORA ........................................................................................................................................................... 4 3 THE FIELD HERBARIUM .......................................................................................................................... -
11 Microbial Communities in the Phyllosphere Johan H.J
Biology of the Plant Cuticle Edited by Markus Riederer, Caroline Müller Copyright © 2006 by Blackwell Publishing Ltd Biology of the Plant Cuticle Edited by Markus Riederer, Caroline Müller Copyright © 2006 by Blackwell Publishing Ltd 11 Microbial communities in the phyllosphere Johan H.J. Leveau 11.1 Introduction The term phyllosphere was coined by Last (1955) and Ruinen (1956) to describe the plant leaf surface as an environment that is physically, chemically and biologically distinct from the plant leaf itself or the air surrounding it. The term phylloplane has been used also, either instead of or in addition to the term phyllosphere. Its two- dimensional connotation, however, does not do justice to the three dimensions that characterise the phyllosphere from the perspective of many of its microscopic inhab- itants. On a global scale, the phyllosphere is arguably one of the largest biological surfaces colonised by microorganisms. Satellite images have allowed a conservative approximation of 4 × 108 km2 for the earth’s terrestrial surface area covered with foliage (Morris and Kinkel, 2002). It has been estimated that this leaf surface area is home to an astonishing 1026 bacteria (Morris and Kinkel, 2002), which are the most abundant colonisers of cuticular surfaces. Thus, the phyllosphere represents a significant refuge and resource of microorganisms on this planet. Often-used terms in phyllosphere microbiology are epiphyte and epiphytic (Leben, 1965; Hirano and Upper, 1983): here, microbial epiphytes or epiphytic microorganisms, which include bacteria, fungi and yeasts, are defined as being cap- able of surviving and thriving on plant leaf and fruit surfaces. Several excellent reviews on phyllosphere microbiology have appeared so far (Beattie and Lindow, 1995, 1999; Andrews and Harris, 2000; Hirano and Upper, 2000; Lindow and Leveau, 2002; Lindow and Brandl, 2003). -
Distribution Pattern Analysis of Epiphytic Bacteria On
Journal of Microscopy and Ultrastructure 2 (2014) 34–40 Contents lists available at ScienceDirect Journal of Microscopy and Ultrastructure jou rnal homepage: www.elsevier.com/locate/jmau Original Article Distribution pattern analysis of epiphytic bacteria on ethnomedicinal plant surfaces: A micrographical and molecular approach ∗ Fenella Mary War Nongkhlaw, S.R. Joshi Microbiology Laboratory, Department of Biotechnology and Bioinformatics, North-Eastern Hill University, Shillong 793022, India a r a t i c l e i n f o b s t r a c t Article history: The epiphytic bacterial community prevalent on ethnomedicinal plant surfaces were stud- Received 29 November 2013 ied for their diversity, niche localization and colonization using the micrographical and Received in revised form 4 February 2014 molecular approaches. Scanning electron microscopy (SEM) revealed the presence of large Accepted 10 February 2014 aggregates of bacterial communities. The bacterial localization was observed in the grooves Available online 5 March 2014 along the veins, stomata and near the trichomes of leaves and along the root hairs. A total of 20 cultivable epiphytes were characterized which were analyzed for richness, evenness and Keywords: diversity indices. Species belonging to the genera Bacillus and Pseudomonas were the most Plant surfaces abundant. Bacillus thuringiensis was the most prevalent epiphyte with the ability to form Epiphytic bacteria biofilm, as a mode of adaptation to environmental stresses. Biofilm formation explains the Microscopy potential importance of cooperative interactions of epiphytes among both homogeneous Biofilm and heterogeneous populations observed under SEM and influencing the development of microbial communities. The study has revealed a definite pattern in the diversity of cultur- able epiphytic bacteria, host-dependent colonization, microhabitat localization and biofilm formation which play a significant role in plant–microbe interaction. -
Antioxidant and Antimicrobial Potential of the Bifurcaria Bifurcata Epiphytic Bacteria
Mar. Drugs 2014, 12, 1676-1689; doi:10.3390/md12031676 OPEN ACCESS marine drugs ISSN 1660-3397 www.mdpi.com/journal/marinedrugs Article Antioxidant and Antimicrobial Potential of the Bifurcaria bifurcata Epiphytic Bacteria André Horta 1,†, Susete Pinteus 1,†, Celso Alves 1, Nádia Fino 1, Joana Silva 1, Sara Fernandez 2, Américo Rodrigues 1,3 and Rui Pedrosa 1,4,* 1 Marine Resources Research Group (GIRM), School of Tourism and Maritime Technology (ESTM), Polytechnic Institute of Leiria, 2520-641 Peniche, Portugal; E-Mails: [email protected] (A.H.); [email protected] (S.P.); [email protected] (C.A.); [email protected] (N.F.); [email protected] (J.S.); [email protected] (A.R.) 2 Higher School of Agricultural Engineering (ETSEA), University of Lleida, E-25003 Lleida, Spain; E-Mail: [email protected] 3 Gulbenkian Institute of Science, 2780-156 Oeira, Portugal 4 Center of Pharmacology and Chemical Biopathology, Faculty of Medicine, University of Porto, 4200-319 Porto, Portugal † These authors contributed equally to this work. * Author to whom correspondence should be addressed; E-Mail: [email protected]; Tel.: +35-1-262-783-325; Fax: +35-1-262-783-088. Received: 31 December 2013; in revised form: 14 January 2014 / Accepted: 4 March 2014 / Published: 24 March 2014 Abstract: Surface-associated marine bacteria are an interesting source of new secondary metabolites. The aim of this study was the isolation and identification of epiphytic bacteria from the marine brown alga, Bifurcaria bifurcata, and the evaluation of the antioxidant and antimicrobial activity of bacteria extracts. The identification of epiphytic bacteria was determined by 16S rRNA gene sequencing. -
Community Structure and Functional Diversity of Epiphytic Bacteria and Planktonic Bacteria on Submerged Macrophytes in Caohai Lake, Southwest of China
Annals of Microbiology (2019) 69:933–944 https://doi.org/10.1007/s13213-019-01485-4 ORIGINAL ARTICLE Community structure and functional diversity of epiphytic bacteria and planktonic bacteria on submerged macrophytes in Caohai Lake, southwest of China Dingbo Yan1,2 & Pinhua Xia1,2 & Xu Song1,2 & Tao Lin1,2 & Haipeng Cao3 Received: 12 February 2019 /Accepted: 22 May 2019 /Published online: 31 May 2019 # Università degli studi di Milano 2019 Abstract Purpose Epiphytic bacteria on the surfaces of submerged macrophytes play an important role in lake biodiversity and ecological processes. However, compared with planktonic bacteria, there is poor understanding of the community structure and function of epiphytic bacteria. Methods Here, we used 16S rRNA gene high-throughput sequencing and functional prediction analysis to explore the structural and functional diversity of epiphytic bacteria and planktonic bacteria of a typical submerged macrophyte (Potamogeton lucens) in Caohai Lake. Results The results showed that the species composition of epiphytic and planktonic bacteria was highly similar as 88.89% phyla, 77.21% genera and 65.78% OTUs were shared by the two kinds of samples. Proteobacteria and Bacteroidetes were dominant phyla shared by the two kinds of communities. However, there are also some special taxa. Furthermore, the epiphytic bacterial communities exhibited significantly different structures from those in water, and the abundant OTUs had opposite constituents. The explained proportion of the planktonic bacterial community by aquatic environmental parameters is significantly higher than that of epiphytic bacteria, implying that the habitat microenvironment of epiphytic biofilms may be a strong driving force of the epiphytic bacterial community. -
Identification Et Profil De Résistance Des Bactéries Autre Que Les Enterobacteriaceae Isolées Des Effluents Hospitaliers
République Algérienne Démocratique et Populaire Ministère de l’Enseignement Supérieur et de la Recherche scientifique Université Blida 1 Faculté des Sciences de la Nature et de la Vie Filière Sciences Biologiques Département de Biologie et Physiologie Cellulaire Mémoire de fin d’étude en vue de l’obtention du diplôme de Master en Biologie Option : Microbiologie-Bactériologie Thème : Identification et profil de résistance des bactéries autre que les Enterobacteriaceae isolées des effluents hospitaliers Présenté par : Soutenu le : 29 /06/2016 Melle HAMMADI Khedidja Mr SEBSI Abderrahmane Devant le jury : Mme. BOKRETA S. MAB , Université Blida 1 Présidente Mme. HAMAIDI F. MCA , Université Blida 1 Promotrice Mme. MEKLAT A. MCA , Université Blida 1 Examinatrice Mr. KAIS H. MA , Université Blida 1 Co-promoteur Année Universitaire : 2015-2016 Remerciements Nous aimerions en premier lieu remercier notre dieu Allah qui nous a donné la volonté et le courage pour la réalisation de ce travail. À notre promotrice, Mme Hamaidi Fella Nous avons eu l’honneur d’être parmi vos étudiants et de bénéficier de votre riche enseignement. Vos qualités pédagogiques et humaines sont pour nous un modèle. Votre gentillesse, et votre disponibilité permanente ont toujours suscité notre admiration. Veuillez bien Madame recevoir nos remerciements pour le grand honneur que vous nous avez fait d’accepter l’encadrement de ce travail. À notre co-promoteur Mr Kais Hichem Votre compétence, a toujours suscité notre profond respect. Nous vous remercions pour votre accueille et vos conseils. Veuillez trouver ici, l’expression de mes gratitudes et de notre grande estime. Nous adressons nos sincères remerciements à Mme Bokrita de nous avoir fait l’honneur de présider le jury examinant ce travail. -
Morphological and Serological Characterization of Bacterial Isolates from Greening/Dieback Diseased Citrus Daniel Carlos Bock University of Wollongong
University of Wollongong Research Online University of Wollongong Thesis Collection University of Wollongong Thesis Collections 1991 Morphological and serological characterization of bacterial isolates from greening/dieback diseased citrus Daniel Carlos Bock University of Wollongong Recommended Citation Bock, Daniel Carlos, Morphological and serological characterization of bacterial isolates from greening/dieback diseased citrus, Doctor of Philosophy thesis, Department of Biology, University of Wollongong, 1991. http://ro.uow.edu.au/theses/1068 Research Online is the open access institutional repository for the University of Wollongong. For further information contact the UOW Library: [email protected] MORPHOLOGICAL AND SEROLOGICAL CHARACTERIZATION OF BACTERIAL ISOLATES FROM GREENING/DIEBACK DISEASED CITRUS A thesis submitted in fulfilment of the requirements for the award of the degree of Doctor of Philosophy from THE UNIVERSITY OF WOLLONGONG by Daniel Carlos Bock B.Sc.C Honours) University of the Witwatersrand, Johannesburg, R.S.A. Department of Biology 1991 ii ABSTRACT The morphological and serological characteristics of bacterial isolates from plants infected with African greening, Reunion greening and Taiwan likubin were investigated. Isolates from Australian citrus dieback affected trees and resembling the putative greening isolates, were also included. Although predominantly long thin rods, the bacterial cells were morphologically variabile in both liquid and plate cultures at 25°C and 35°C. "Round forms" developed with age and nutrient limitations. Based on colony morphology and pigmentation, the isolates were categorized into two groups - Groups 1 and 2. Whole cell protein patterns were obtained by SDS-PAGE. Patterns of the Group 1 isolates, which were conserved with growth, were similar to one another and different from the patterns of the Group 2 isolates. -
Diplomová Práce
Univerzita Palackého v Olomouci Diplomová práce Olomouc 2018 Bc. Lucie Bílková Univerzita Palackého v Olomouci Přírodovědecká fakulta Katedra buněčné biologie a genetiky Rekonstrukce kompletní chloroplastové DNA u vybraných rostlinných druhů a jejich komparativní analýza Diplomová práce Bc. Lucie Bílková Studijní program: Biologie Studijní obor: Molekulární a buněčná biologie Forma studia: Prezenční Olomouc 2018 Vedoucí práce: Mgr. Eva Hřibová, Ph.D. Prohlašuji, ţe jsem diplomovou práci vypracovala samostatně pod vedením Mgr. Evy Hřibové, Ph.D. a za pouţití uvedených literárních zdrojů. V Olomouci Lucie Bílková i Souhrn Předloţená diplomová práce se zabývá rekonstrukcí kompletní chloroplastové DNA u vybraných taxonů rodu Dactylorhiza a jejich anotací a následnou komparativní analýzou. Předmětem teoretické části diplomové práce bylo vypracovat literární rešerši, která se zaměřuje na strukturu a organizaci genomu vyšších rostlin, zejména na mimojadernou DNA, konkrétně chloroplastovou DNA, její vyuţití a sekvenační přístupy vyuţívané pro sestavení celogenomové sekvence chloroplastové DNA. V praktické části byla analyzována Next-Gen data (Illumina sekvence), provedena de-novo rekonstrukce chloroplastové DNA s následným in silico i experimentálním ověřením. Byla rovněţ provedena anotace kompletní chloroplastové DNA a fylogenetická analýza. Podařilo se zrekonstruovat celkový genom chloroplastové DNA u osmi studovaných taxonů rodu Dactylorhiza. U druhu Dactylorhiza fuchsii subsp. soóana se povedlo sloţit celkový clDNA genom v jednom dlouhém scaffoldu. Pouze u jediného druhu, Dactylorhiza bohemica, se nepodařilo sloţit celkový genom clDNA. Na základě provedených analýz bylo zjištěno, ţe velikost chloroplastové DNA studovaných taxonů rodu Dactylorhiza je 154 113-156 724 kb. Proteiny kódující geny, tRNA a rRNA tvořily zhruba 70 % celého genomu chloroplastu. Z toho připadalo zhruba 48 % na proteiny kódující geny, 19 % na geny pro tRNA a 3 % na geny pro rRNA. -
UJI AKTIVITAS ANTIBAKTERI ISOLAT DAN HIDROLISAT PROTEIN KERANG DARAH (Anadara Granosa L)
UJI AKTIVITAS ANTIBAKTERI ISOLAT DAN HIDROLISAT PROTEIN KERANG DARAH (Anadara granosa L) SKRIPSI HALAMAN JUDUL OLEH : VIVIENNE WIJAYA NIM 171501050 PROGRAM SARJANA FARMASI FAKULTAS FARMASI UNIVERSITAS SUMATERA UTARA MEDAN 2021 i UJI AKTIVITAS ANTIBAKTERI ISOLAT DAN HIDROLISAT PROTEIN KERANG DARAH (Anadara granosa L) SKRIPSI Diajukan sebagai salah satu syarat untuk memperoleh gelar Sarjana Farmasi pada Fakultas Farmasi Universitas Sumatera Utara OLEH : VIVIENNE WIJAYA NIM 171501050 PROGRAM SARJANA FARMASI FAKULTAS FARMASI UNIVERSITAS SUMATERA UTARA MEDAN 2021 ii PENGESABAN SKRIPSI un AK11VIT AS ANTIBAKTERI ISOLAT DAN BIDROLISAT PROTEIN KERANG DARAH (A • JNII.. L) OLI.II: VIVIENNE WUA YA NIM 171!11158 Dipertahanbn di Hadapln Penguji Skripli Fakultas Fanwi Uui\'asibd Sumlten Utara pada TIIIIPI: OI Juni 2021 Disetujui oleh: b"ig) Panitia "'-JL :· ~ ~Sri Yul" i. S.Fann.,M.Si., Apt Prof.Dr.rer.nat.Effi:ady De Lux Pun, SU., Apt NIP. 198207032001122002 NIP. 195306191913031001 Pembimbing II. Sri ~.Fam.,M.Si, Apt NIP. l 98207032008122002 YodeW:.::.-:.Farm, M.Si, Apt. NIP. 191704212017042001 Dr. SitOnls, M.Si., Apt. Ketua Pqnm Studi Sarjana Farmasi, NIP. 195310301980031002 Dr. Sumaiyah,- .Si., Apt. ftnlna1a, S.f11111., M.Si .. Apt. NIP. l 977 l 2262008122002 7042120 17042001 ., ~ Pa.I)., Apt. l S2008122001 iii KATA PENGANTAR Puji syukur kehadirat Tuhan Yang Maha Esa yang telah memberikan rahmat dan karunia-Nya sehingga penulis dapat menjalani penelitian hingga akhirnya menyelesaikan penyusunan skripsi yang berjudul “Uji Aktivitas Antibakteri Isolat dan Hidrolisat Protein Kerang Darah (Anadara granosa L.)”. Skripsi ini diajukan sebagai salah satu syarat untuk memperoleh gelar Sarjana Farmasi pada Fakultas Farmasi Universitas Sumatera Utara. Penulis menyampaikan ucapan terimakasih kepada Ibu Sri Yuliasmi, S.Farm., M.Si., Apt. -
Diplomová Práce Vytrvalé Slunečnice (Helianthus
Mendelova univerzita v Brně Zahradnická fakulta v Lednici Diplomová práce Vytrvalé slunečnice ( Helianthus L.): historie pěstování a hodnocení sortimentů Vedoucí práce Vypracovala doc. Dr. Ing Jiří Uher Bc. Kateřina Tejkalová Čestné prohlášení Prohlašuji, že jsem práci: Vytrvalé slunečnice (Helianthus L.): historie pěstování a hodnocení sortimentu vypracoval/a samostatně a veškeré použité prameny a informace uvádím v seznamu použité literatury. Souhlasím, aby moje práce byla zveřejněna v souladu s § 47b zákona. 111/1998 Sb., o vysokých školách ve znění pozdějších předpisů a v souladu s platnou Směrnicí o zveřejňování vysokoškolských závěrečných prací. Jsem si vědom/a, že se na moji práci vztahuje zákon. 121/2000 Sb., autorský zákon, a že Mendelova univerzita v Brně má právo na uzavření licenční smlouvy a užití této práce jako školního díla podle § 60 odst. 1 autorského zákona. Dále se zavazuji, že před sepsáním licenční smlouvy o využití díla jinou osobou (subjektem) si vyžádám písemné stanovisko univerzity, že předmětná licenční smlouva není v rozporu s oprávněnými zájmy univerzity, a zavazuji se uhradit případný příspěvek na úhradu nákladů spojených se vznikem díla, a to až do jejich skutečné výše. V Brně dne: 7. 5. 2015 .………………………….. podpis Poděkování Děkuji za odborné rady a pomoc vedoucímu diplomové práce, panu doc. Dr. Ing. Jiřímu Uhrovi. Dále děkuji konzultantovi panu Petru Hanzelkovi za poskytnutí rostlinného materiálu, stejně tak i Botanické zahradě Praha Troja, Olomouc a školkám Litomyšl. Děkuji také své rodině za podporu a -
Rangelands, Western Australia
Biodiversity Summary for NRM Regions Species List What is the summary for and where does it come from? This list has been produced by the Department of Sustainability, Environment, Water, Population and Communities (SEWPC) for the Natural Resource Management Spatial Information System. The list was produced using the AustralianAustralian Natural Natural Heritage Heritage Assessment Assessment Tool Tool (ANHAT), which analyses data from a range of plant and animal surveys and collections from across Australia to automatically generate a report for each NRM region. Data sources (Appendix 2) include national and state herbaria, museums, state governments, CSIRO, Birds Australia and a range of surveys conducted by or for DEWHA. For each family of plant and animal covered by ANHAT (Appendix 1), this document gives the number of species in the country and how many of them are found in the region. It also identifies species listed as Vulnerable, Critically Endangered, Endangered or Conservation Dependent under the EPBC Act. A biodiversity summary for this region is also available. For more information please see: www.environment.gov.au/heritage/anhat/index.html Limitations • ANHAT currently contains information on the distribution of over 30,000 Australian taxa. This includes all mammals, birds, reptiles, frogs and fish, 137 families of vascular plants (over 15,000 species) and a range of invertebrate groups. Groups notnot yet yet covered covered in inANHAT ANHAT are notnot included included in in the the list. list. • The data used come from authoritative sources, but they are not perfect. All species names have been confirmed as valid species names, but it is not possible to confirm all species locations.