DOSSIER FMD Epidemiological Situation in Bulgaria

MINISTRY OF AGRICULTURE AND FOOD BULGARIAN FOOD SAFETY AGENCY * Sofia, 1606, “Pencho Slaveikov” blvd. 15 ( +359 (0) 2 915 98 20, +359 (0) 2 954 95 93, www.bfsa.government.bg

DOSSIER

FMD epidemiological situation in Bulgaria

Application by the Bulgarian Food Safety Agency for recovery of the former status of whole theritory of Bulgaria as a “COUNTRY FREE FROM FMD WITHOUT VACCINATION”.

Sofia, Bulgaria 14/11/2011

1 Content

1. FMD eradication ………………………………………………………………………………………………… 3 2. FMD surveillance. Surveillance strategy and measures applied in relation with all FMD outbreaks…………………………………………………………………………………………………………… 18 3. Epidemiological findings and considerationsin relation with all outbreaks ……………………………………………………………………………………………………………………… …20 4. Next steps……………………………………………………………………………………… … 21 5. Annexes: - Annex I - surveillance in 3km protective and 10 km surveillance zones of the outbreak Kost (IP-1)i, Tsarevo municipality,Burgas region………………………………………………… 25 - Annex II - surveillance in 3km protective and 10 km surveillance zones of the outbreak Rezovo (IP- 2), Tsarevo municipality, Burgas region……………………………………… ……………29 - Annex III - surveillance in 3km protective and 10 km surveillance zones of the outbreak Gramatikovo(IP-3),Malko Turnovo municipality, Burgas region…………………… ..32 - Annex IV - surveillnace in the settlements of Burgas region outside the protection surveillance zones around the outbreaks - time period – 05.01-27.02.2011………… …………35 - Annex V - surveillnace in 3km protective and 10 km surveillance zones of the outbreak Kirovo(IP-4), Sredetc municipality, Burgas region - second outbreak wave……………… ….52 - Annex VI - surveillnace in 3km protective and 10 km surveillance zones of the outbreak Granichar(IP-5), Sredetc municipality, Burgas region - second outbreak wave……… 54 - Annex VII - surveillnace in 3km protective and 10 km surveillance zones of the outbreaks Golyamo Bukovo(IP-6), Fakiya(IP-7) and Momina Tsurkva(IP-8), Sredets municipality, Burgas region - second outbreak wave…………………………………………………………… 56 - Annex VIII - surveillnace in 3km protective and 10 km surveillance zones of the outbreaks Bliznak village (IP-9) and Bliznak buffalo farm (IP-10), Malko Turnovo municipality, Burgas region - second outbreak wave…………………………………………………………… 59 - Annex IX - surveillnace in 3km protective and 10 km surveillance zones of the outbreaks Dolno Yabalkovo(IP-11),Sredets municipality Burgas region - second outbreak wave… 61 - Annex X - surveillance in the framework of disease surveillance after the last outbreaks– time period 18.04-19.04.2011……………………………………………………………………… .. 66 - Annex XI - surveillance in the framework of disease surveillance after the last outbreaks– time period 28 - 29 .04.2011…………………………………………………………… 75 - Annex XII - surveillance in the framework of disease surveillance after the last outbreaks– time period 18 -19.05.2011…………………………………………………………… 79 - Annex XIII - surveillance in the framework of disease surveillance after the last outbreaks– time period 16 - 25.08.2011…………………………………………………………… 85 - Annex XIV - surveillance in wild animals - time period January – August 2011……… 97 - Annex XV - time of FMD events in Bulgaria 2010 – 2011……………………………… 99

2 1. FMD eradication. a) History. Chronology of the FMD cases and outbreaks in the country.

Ø On 5th of January 2011 -date of first case of FMD in one wild boar shot on 30 December 2010 about 2 km north from the Turkish border in the vicinity of the village of Kosti (Tsarevo municipality, Burgas region).

Ø On 9th of January in the village of Kosti (IP-1), Tsarevo municipality, in the vicinity of the place were the wild boar was shot;

Ø On 15th of January in the village of Rezovo (IP-2), Tsarevo municipality, located southeast of Kosti at the border with Turkey;

Ø On 31st of January in the village Gramatikovo (IP-3), Malko Tarnovo municipality, situated 10 km west of Kosti within the surveillance zone around IP-1 in Kosti;

Ø On 19th of March in a bovine farm nearby the village of Kirovo (IP-4), Sredets municipality, 7 km north of the border with Turkey;

Ø On 24th of March in the village of Granichar (IP-5),Sredets municipality, 3 km north of the border with Turkey;

Ø On 25th of March o in a mixed bovine and pig farm nearby the village of Goliamo Bukovo (IP-6), Sredets municipality, 5 km northeast of IP-4, within the surveillance zone around the Kirovo outbreak;

o in a bovine farm nearby the village of Fakia (IP-7), Sredets municipality, west of the Kirovo outbreak, 10 km north of the border with Turkey;

o in a bovine farm nearby the village of Momina Tsarkva (IP-8), Sredets municipality west of IP-7, 6 km north of the border with Turkey;

Ø On 3rd of April o in the village of Bliznak (IP-9), Malko Tarnovo municipality 7 km north of the border with Turkey;

o in a grazing buffalo herd 2 km south of Bliznak village (IP-10), Malko Tarnovo municipality in about 5 km north of the border with Turkey;

Ø On 7th of April in Dolno Yabalkovo (IP-11), Sredets municipality in village herd of sheep, goats and a bovine.

3 Bulgaria Black sea

7 4 6 9 8 11 5 10 3 1 2 WB

Turkey Greece

Figure 1. (a) WB - wild boar shot 30.12.2010 (b) Location of the FMD outbreaks in Burgas region : 1. Kosti; 2. Rezovo; 3. Gramatikovo, 4. Kirovo; 5. Granichar; 6. Goliamo Bukovo; 7. Fakia; 8. Momina Tsarkva; 9. Bliznak; 10. Herd 2 km from Bliznak; 11. Dolno Yabalkovo b) Chronology of the events, strategy and applied measures related to the control and eradication of FMD in all outbreaks

05.01.2011 On 30.12.2010 three wild boars were shot 2 km north from the Turkish border in the area of Makevtci, on the land of Kosti village (Tsarevo municipality, Burgas region) (see Figure 1). Acute clinical signs for FMD in the feet (coronary band of claws) only in one of the 3 wild boar have been observed. Samples ( tissue samples) from the 3 wild boar were tested on 4.01.2011 by lateral flow device for FMDV-antigen detection, Ag ELISA- type O and RT PCR-real time

Ø The samples only from one of tested wild boar showed positive results, as follow:Lateral flow device for FMDV-antigen detection- positive; Ø Ag ELISA- type O - positive; Ø RT PCR-real time- protocols by Scott Read and Kalahan- positive for presence of the FMD viral genome.

On 5 January 2011 the first case of FMD in one wild boar was declared.

On 07.01.2011 the samples were sent to of the EU Reference Laboratory (EU-RL), Institute for Animal Health, Pirbright, United Kingdom, for confirmation and isolation virus. The analysis made from the samples (WB)indicate this FMD virus is a serotype O, Topotype ME- SA, Strain PanAsia-2ANT-10 which has more than 99.5% affinity with isolates collected in 2010 in Iran and Turkey.

4 05.01.2011 Start the implementation of the measures for control and eradication of FMD according to Council Directive 2003/85/EC.

Portection (3km) and surveillance (10 km) zones were established around Kosti and clinical and epidemiological examinations as well as blood sampling of susceptible animals in all villages located in the municipalities of Tsarevo, Malko Tarnovo and Sredets. (samples in tables in Annex II).

08.01.2011 By COMMISSION DECISION 2011/8/EU of 6 January 2011 concerning certain interim protection measures against foot-and-mouth disease in Bulgaria, some regions of the country were separated as a zone with high risk Annex I (the entire region of Burgas) and zones with low risk Annex II (the regions of Yambol, Sliven, Shumen and Varna). No susceptible animals were allowed to move from these zones to other territories of Bulgaria. No FMD susceptible animals and products thereof could leave the territories of Annex I.

Decision 2011/8/EU

Figure 2 Annex I and Annex II areas accroding to Decision 2011/8/EU

09.01.2011

On 09.01.2011 the National Reference laboratory (NRL) for FMD confirmed positive for NSP ELISA and antibodies FMD type O ELISA blood samples taken on 07-08.01.2011 during surveillance activities (Figure 4 (b) in animals from Kosti (Tsarevo municipality, Burgas region). The results are as follow: Cattle - 1 positive for NSP ELISA and antibodies FMD type O ELISA Sheep – 14 positives for NSP ELISA and antibodies FMD type O ELISA Goats – 12 positives for NSP ELISA tests and antibodies FMD type O ELISA Pigs – 8 positives for NSP ELISA

The Veterinary service (National Veterinary Service (NVS) started the implementation of the measures for the control and eradication of FMD according to Council Directive 2003/85/EC, namely:

5 - On 10.01.2011 by Order of the Director General of NVS, establishment of the 3 km protective and 10 km surveillance zones around Kosti village and the relevant restrictive measures were imposed. By Order of the Executive Director of the Bulgarian Food Safety Agency (BFSA) (BFSA is a newly established agency, in operation since March 2011, wich included all structures of the NVS) on 04.07.2011 the 3 km protective and 10 km surveillance zones were removed. - On 10.01.2011 by Order of the Director General of NVS, NVS started killing of all susceptible animals in the village of Kosti. The killing of animals took 3 days, because of difficulties to round up 2 free range cattle herds. - Cleaning and disinfection on the spot. After killing of animals. The empty farms, animal holdings, backyards, roads, pastures were disinfected several times. - On 10.01.2011 start of the clinical examination and blood sampling in the village in the 3 km protective and 10 km surveillance zones around Kosti.

Map of the outbreak, the villages in 3 km protective and 10 km surveillance zones, the number of animals present in the villages, and the serological surveillance are listed in Annex I

15.01.2011 Since the confirmation of FMD in wild boar on 05.01.11 NVS started clinical and epidemiological examinations as well as blood sampling of susceptible animals in all villages located in the municipalities of Tsarevo, Malko Tarnovo and Sredets in Burgas region. During the clinical examinations under the surveillance a suspicion of FMD in Rezovo(Tsarevo municipality, Burgas region) (Figure 4 (2)) was detected on 15.01.2011.

NVS started the measures in relation with the suspicion according the Directive 2003/85 on 15.01.2011.Clinical sings for FMD were observed in cattle and the laboratory results confirmed the disease on 17.01.2011, as follow: Cattle - 19 samples positive for RT-PCR, 12 positive for Ag ELISA FMD Pigs - 3 positive Ag ELISA FMD

After the confirmation of FMD in Rezovo on 17.01.2011 NVS started the implementation of the measures for control and eradication of FMD according to Council Directive 2003/85/EC, namely: - On 17.01.2011 by Order of the Director General of NVS 3 km protective and 10 km surveillance zones were defined around Rezovo village and the relevant restrictive measures were imposed. By Order of the Executive Director of BFSA on 04.07.2011 the 3 km protective and 10 km surveillance zones were removed. - On 17.01.2011 by Order of the Director General of NVS, NVS started killing of all susceptible animals in the village of Rezovo. The killing of animals took 2 days because of difficulties to round up free range cattle . - Furthermore, on the grazing grounds of Rezovo a herd of 21 Turkish buffalos were found. The buffalos entered into Bulgaria in the beginning of January (4.01.2011) and were grazing together with the cattle from Rezovo. All 21 buffaloes were killed on 20.01.2011 and lesions of FMD were observed during the killing in one of the animal (yonger than 12 months of age, without eartag). - Cleaning and disinfection on the spot. After killing of the animals the empty farms, animal holdings, backyards, roads, pastures were disinfected several times. - On 17.01.2011 start of the clinical examination and blood sampling in the village located in 10 km surveillance zones around Rezovo ( no villages in 3 km protective )

6 Map of the outbreak, the villages in 3 km protective and 10 km surveillance zones, the number of animals present in the villages the blood samples taken from these animals are listed in Annex II

19.01.2011 By COMMISSION DECISION 2011/44/EU of 19 January 2011 concerning certain protection measures against foot-and-mouth disease in Bulgaria, two more regions (Kardjali and Haskovo) were included in the zones with low risk (Annex II). Pursuant to the Decision no live FMD susceptible animals and products thereof could live the territories of Annex I and no live FMD susceptible animals were allowed to leave the territories of Annex II.

Fig 3 Annex I and Annex II areas accroding to Decision 2011/44/EU

31.01.2011 21 days after the confirmed outbreak in Kosti (IP-1) and during the second round of clinical and serological testing in the establiched 10 km surveillance zone around the outbreak (IP-1), new suspicion of FMD in Gramatikovo (Malko Tarnovo municipality, Burgas region) (Figure 4 (d) was detected on 30.01.2011.

NVS started the measures in relation with the suspicion according to the Directive 2003/85 on 30.01.2011.

On 31.01.2011 the FMD NRL confirmed the disease: Cattle - 1 positive for NSP ELISA and FMDAb O type ELISA Sheep – 13 positive for NSP ELISA and Moab O type ELISA. Goats – 10 positive for NSP ELISA and Moab O type ELISA.

After the confirmation of FMD in the village on 31.01.2011 NVS started the implementation of the measures for control and eradication of FMD according to Council Directive 2003/85/EC, namely: - On 31.01.2011 by Order of the Director General of NVS 3 km protective and 10 km surveillance zones were defined around Gramatikovo village and the relevant restrictive measures were imposed. By Order of the Executive Director of BFSA on 04.07.2011 the 3 km protective and 10 km surveillance zones were removed.

7 - On 31.01.2011 by Order of the Director General of NVS, NVS started killing of all susceptible animals in the village of Gramatikovo. On 01.02.2011 all susceptible animals were killed. - Cleaning and disinfection were carried out on the spot. Several disinfection was carried out in the empty farms, animal holdings, backyards, roads, and pastures after killing of the animals. - On 31.01.2011 start of the clinical examination and blood sampling in the village in 3 km protective and 10 km surveillance zones around Gramatikovo.

Map of the outbreak, the villages in 3 km protective and 10 km surveillance zones, the number of animals present in the villages and the surveillance are listed in Annex III

Fig. 4. Location of the FMD outbreaks in Burgas region; a) WB wild boar shot; b) 1. Kosti; c) 2. Rezovo; d) 3. Gramatikovo

31.01.2011 – 28.02.2011 Clinical examinations and blood sampling of susceptible animals in all villages in the risk municipalities of Burgas region. In the villages (nearby the Turkish border and in the villages within the 3 km protection zones and 10 km surveillance zones around the 3 outbreaks all susceptible animals were blood sampled. Susceptible animals in all other villages outside the 3 km protection zones and 10 km surveillance zones around the 3 outbreaks in Burgas region were sampled according to an estimated 5 % sero-prevalence and 95% confidence. (Please see Annex IV)

01.03.2011 – 18.03.2011

8 Clinical examinations of susceptible animals in all the villages in the risk municipalities of Burgas region, on the base of the requirements of Annex III of the Directive 2003/85/EC

18.03.2011 On 18.03.2011 infected animals were detected in a bovine farm of 143 cattle, during the clinical surveillance (time period; 01.03.2011 to 18.03.2011) of susceptible animals in the villages in the risk municipalities of Burgas region. The bovine farms is located at about 3 km south of the village of Kirovo, Sredets municipality, Burgas region, 7 km north of the border with Turkey and 42 km from the last outbreak in Gramatikovo (IP-3) ( fig 5 and 5a). The bovines were bred extensively in fenced forest area.

About 28 of 60 inspected that day bovines in the farm showed clinical signs of FMD (salivation and mouth lesions 4-5 days old ).

The owner of the affected farm observed changes in the health status in several cattle 2 days ago. He presumed that the reason of abnormal behaviour of animals is because of feeding with non quality hay which was the reason for the delayed notification to the veterinary service.

Since January 2011 the aforementioned bovine herd and the animals in Kirovo village were clinical examinated and blood sampled 5 rounds with negative results (07-14.01.2011; 28.01- 03.02.2011; 07-10.02.2011, 16-20.02.2011 – NSP negative and 24-28.02.2011 negative for antibody to SP of the FMDV (Mono ELISA O-type Prionics)-see Annex IV).

Fig. 5. Location of the infected farm (IP-4 outbreak-Kirovo) and the distance from IP-3 –Gramatikovo)

9 Fig. 5a. Location of the infected farm (IP-4 outbreak-Kirovo) and with 3 km protective and 10 km surveillance zones around the outbreak

NVS started the measures in relation with the suspicion according to the Directive 2003/85 on 18.03.2011.

On 19th March the NRL confirmed the diagnosis (15 samples RT PCR positive)

BSFA started the measures for control and eradication of FMD pursuant to Directive 2003/85/EC on 19.01.2011: - On 19.03.2011 by Order of the Executive Director of BFSA 3 km protective and 10 km surveillance zones were defined around Kirovo village and the relevant restrictive measures were imposed. By Order of the Executive Director of BFSA on 04.07.2011 the 3 km protective and 10 km surveillance zones were removed. - Killing of animals was ordered on 19.03.2011 by Order of the Executive Director of BFSA. On 19.03.2011 BFSA started killing of all susceptible animals in the village of Kirovo. By reason of the type of animal husbandry in the area (free range) the time of killing took 3 days. The farms occupy an area of 400dka size and the persons responsible for the killing of animals had some difficulties to perform theirs duties. - Cleaning and disinfection were carried out on the spot. Several disinfection was carried out in the empty farms, animal holdings, backyards, roads, and pastures after killing of the animals. - On 19.03.2011 start of the clinical examination and blood sampling in the village in 3 km protective and 10 km surveillance zones around Kirovo.

Map of the outbreak, the villages in 3 km protective and 10 km surveillance zones, the number of animals present in the villages the surveillance carried out are listed in Annex V

23.03.2011 Infected bovine herd detected in Granichar village (Sredets municipality, Burgas region) (IP- 5) (Figure 6) in the framework of routine clinical examinations carried out in the established

10 10 km surveillance zones around Kirovo outbreak ( IP-4). The village is located at a distance of 3km from the border with Turkey. In the village there was only one herd with 133 bovines. No other susceptible animal species were kept there.

15 bovines from the herd disiplayed clinical signs of FMD (salivation and about 4-5 days old mouth lesions). The owner of the affected farm did not observed any changes in the health status of the cattle. The animals were kept in free range area and the owner could observe these animals only in case of additional feeding.

Since January 2011 the bovine herd was clinical examinated and blood sampled in 5 rounds with negative results (08Jan, 28Jan, 07Feb, 16Feb - negative for NSP antibodies to FMD and 25Feb - negative for antibody to SP of the FMDV (Mono ELISA O-type Prionics)-see Annex IV. All clinical examination in the herd carried out during the period 28.02-18.03.2011 were negative for FMD clinical signs.

On 23.03.2011 BFSA started the measures in relation with the suspicion according to the Directive 2003/85

On 24th March the NRL in Sofia confirmed the diagnosis: 3 positives under RT PCR and 7 positives under FMDAb O type ELISA

Fig6. Location of the outbreaks Granitchar and Kirovo (infected farm) in Sredets municipality, Burgas region with the 3 km protection and 10 km surveillance zones around the two outbreaks

BSFA started the measures for control and eradication of FMD pursuant to Directive 2003/85/EC on 24.03.2011: - On 24.03.2011 by Order of the Executive Director of BFSA 3 km protective and 10 km surveillance zones were defined around Granitchar. village and the relevant restrictive measures were imposed. By Order of the Executive Director of BFSA on 04.07.2011 the 3 km protective and 10 km surveillance zones were removed.

11 - On 24.03.2011 by Order of the Executive Director of BFSA, BFSA started killing of all susceptible animals in the village of Granitchar. The killing of animals took 1day. - Cleaning and disinfection were carried out on the spot. Several disinfection was carried out in the empty farms, animal holdings, backyards, roads, and pastures after killing of the animals. - On 24.03.2011 start of the clinical examination and blood sampling in the village in 3 km protective and 10 km surveillance zones around Granichar.

Map of the outbreak, the villages in 3 km protective and 10 km surveillance zones, the number of animals present in the villages the blood samples taken from these animals are listed in Annex VI

24.03.2011 On 24th March three new suspicions detected in the municipality of Sredets by FMD clinical signs in the framework of clinical examinations in villages Goliamo Bukovo, Fakia, Momina Tsurkva (fig.7). Suspisions in Goliamo Bukovo and Fakia were raised in the framework of clinical examinations carried out in the established 10 km surveillance zone around Kirovo outbreak ( IP-4). The suspicion in Momina Tsurkva arised from additional and extended clinical surveillance carried out in Sredets municipality.

On 24.03.2011 BFSA started the measures in relation with the suspicion according to the Directive 2003/85 in three villages in Sredets municipality. The herds in these 3 villages were blood sampled 5 rounds with negative results (07-14.01.2011; 28.01-03.02.2011; 07- 10.02.2011, 16-20.02.2011 - negative for NSP antibodies to FMD and 24-28.02.2011 - negative for antibody to SP of the FMDV (Mono ELISA O-type Prionics)-see Annex IV) since January.

The FMD diagnosis was confirmed by NRL /PCR and Ag ELISA positive, Ab negative) on 25th March as follow:

IP-6 Outbreak in mixed cattle and pigs farm (49 cattle and 11 pigs) located nearby Goliamo Bukovo village, the village is located in the 10km surveillance zone around IP-4 the outbreak in Kirovo: 4 positive cattle under RT-PCR, 3 of them FMD Ag ELISA positive, all 4 tested animals negative for antibody against SP of the FMDV (Mono - O type ELISA Prionics)

IP-7 Bovine farm (81 cattle) nearby the village of Fakia. One animal was confirmed positive under RT-PCR and FMD Ag ELISA. The village is located in the 10km surveillance zone around the outbreak Kirovo (IP 4)

IP-8 Bovine farm (209 cattle) nearby Momina Tsarkva village. Two amiamls were confirmed positive under RT-PCR and FMD Ag ELISA. The village is located in the 10km surveillance zone around the outbreak Fakia (VII outbreak).

12 Figure 7 Location of the three outbreaks ( IP-6, IP-7, IP-8) in Sredets municipality, Burgas region with the 3 km protection and 10 km surveillance zones .

On 25.03.2011, BSFA started the measures for control and eradication of FMD pursuant to Directive 2003/85/EC:

- On 25.03.2011 by Order of the Executive Director of BFSA 3 km protective and 10 km surveillance zones were defined around the villages and the relevant restrictive measures were imposed. The 3 km protective and 10 km surveillance zones were removed by Order of the Executive Director of BFSA on 04.07.2011. - The killing of susceptible animals in the infected farms was ordered on 25.03.2011 by Order of the Executive Director of BFSA. The killing of animals took 1 day per outbreak. - Cleaning and disinfection were carried out on the spot. Several disinfection was carried out in the empty farms, animal holdings, backyards, roads, and pastures after killing of the animals. - On 25.03.2011 start of the clinical examination and blood sampling in the village in 3 km protective and 10 km surveillance zones around the outbreaks.

Map of the outbreak, the villages in 3 km protective and 10 km surveillance zones, the number of animals present in the villages the blood samples taken from these animals are listed in Annex VIII

26.03.2011 Implementation of measures for control and eradication of FMD according to Council Directive 2003/85/EC; clinical and epidemiological examinations as well as blood sampling

13 of susceptible animals in the risk villages located in Burgas and the neighboring to the west Yambol region.

03.04.2011 Two new outbreaks (fig 8) detected in the municipality of Malko Tarnovo in the framework of serological surveillance carried out in the established 10km surveillance zones around Granichar outbreak (IP-5):

IP-9 Bliznak village – located in the 10km surveillance zones around Granichar (IP-5) and the farm near Goliamo Bukovo (IP-6). The animals kept in the village were clinical examinated and blood sampled 5 rounds with negative results (07-12.01.2011; 28.01- 09.02.2011;15-20.02.11 negative for NSP antibodies to FMD; 25-28.02.2011;19.03- 30.03.2011 negative for antibody to SP of the FMDV) since January. By phone conversation the NRL notified BFSA about Ab FMD positive results on 03.04.2011, as follow:

Cattle - 21 samples FMD ELISA Type O Priocheck and FMD NSP ELISA positive Sheep - 42 samples FMD ELISA Type O Priocheck and FMD NSP ELISA positive Goat’s - 36 samples FMD ELISA Type O Priocheck and FMD NSP ELISA positive

IP-10 Mixed buffalo,, sheep and goats farm 2 km south of Bliznak (Malko Tarnovo municipality) in about 3 km north of the border with Turkey. On 03.04.2011 NRL informed by phone BFSA about Ab FMD positive results obtained in the framework of serological surveillance, as follow:

Buffalos – 56 samples positive under ELISA Type O Priocheck , 43 positive for FMD NSP ELISA Sheep - 20 samples positive under ELISA Type O Priocheck and FMD NSP ELISA Goats - 36 positive under ELISA Type O Priocheck and FMD NSP ELISA.

14 1 2

Fig 8. Location of the two outbreaks ( IP-9 and IP-10) in Malko Turnovo municipality, Burgas region with the 3 km protection and 10 km surveillance zones . On 03.04.2011 BFSA started the implementation of measures for control and eradication of FMD in the outbreaks according to Directive 2003/85/EC

- 3 km protective and 10 km surveillance zones were defined around the both outbreaks and the relevant restrictive measures were imposed by Order of the Executive Director of BFSA on 04.04.2011 (because the official letters from the FMD NRL concerning the positive results are from 04.04.2011). By Order of the Executive Director of BFSA on 04.07.2011 the 3 km protective and 10 km surveillance zones were removed. - The killing of susceptible animals in the infected farms was ordered on 04.04.2011 by Order Executive Director of BFSA. The killing of animals took 2 days - Cleaning and disinfection were carried out on the spot. Several disinfection was carried out in the empty farms, animal holdings, backyards, roads, and pastures after killing of the animals. - On 04.04.2011 start of the clinical examination and blood sampling in the village in 3 km protective and 10 km surveillance zones around the outbreaks.

Map of the outbreaks, the villages in 3 km protective and 10 km surveillance zones, the number of animals present in the villages the blood samples taken from these animals are listed in Annex IX

07.04.2011 During the serological surveillance carried out in the established 3 km protective zones around Kirovo (IP-4) i Granichar outbreak (IP-5) new outbreak was detected on 7th of April in

15 herd of sheep, goats and a bovine in Dolno Yabalkovo village (IP-11), Sredets municipality (fig 9).

Since January this herd has been clinical examinated and blood sampled for evidence of sero- conversion with negative results 6 times (07-14.01.2011; 28.01-03.02.2011; 07.02- 10.02.2011, 16-20.02.2011 negative for NSP antibodies to FMD, 24-28.02.11, 19.03- 30.03.2011 negative for antibody to SP of the FMDV – see Annex IV), but was now tested sero-positive when re-sampled on the 4th of April.

On 07.04.2011 the NRL notified BFSA for the Ab FMD positive samples, as follow: Cattle - 1 SP of the FMDV (Mono ELISA O-type Prionics) positive animal. Goats/sheep - 10 SP of the FMDV ( Mono ELISA O-type Prionics) positives

Fig 9. Location of the outbreaks (IP-10) in Sredets municipality, Burgas region with the 3 km protection and 10 km surveillance zone

On 07.04.2011 BFSA started the implementation of measures for control and eradication of FMD according to Directive 2003/85/EC: - 3 km protective and 10 km surveillance zones were defined around the outbreak and the relevant restrictive measures were imposed by Order of the Executive Director of BFSA on 07.04.2011. By Order of the Executive Director of BFSA on 04.07.2011 the 3 km protective and 10 km surveillance zones were removed. - On 07.04.2011 according to the Order issued by the Executive Director of BFSA the killing of the susceptible animals started. The killing of animals took 3 days.

16 - Cleaning and disinfection were carried out on the spot. Several disinfection was carried out in the empty farms, animal holdings, backyards, roads, and pastures after killing of the animals. - On 07.04.2011 start of the clinical examination and blood sampling in the village in 3 km protective and 10 km surveillance zones around the outbreak.

Map of the outbreak, the villages in 3 km protective and 10 km surveillance zones, the number of animals present in the villages the blood samples taken from these animals are listed in Annex X.

18.04.11-19.04.2011 Clinical examinations and blood sampling of susceptible animals in the villages in the risk municipalities of Burgas and Yambol regions as well as in other villages in Burgas, Varna, Shumen, Sliven, Yambol and Haskovo regions for proving absence of FMD virus circulation in domestic livestock in the areas north and west of the detected outbreaks.(Please see Annex XI) 28.04.2011 – 29.04.2011 Clinical examinations and blood sampling of susceptible animals in the villages in the risk municipalities of Burgas and Yambol regions. . (Please see Annex XII)

18.05.2011 – 19.05.2011 Clinical examinations and blood sampling of susceptible animals in the villages in the risk municipalities of Burgas and Yambol regions as well as in other villages in Burgas, Varna, Shumen, Sliven, Yambol and Haskovo regions for proving absence of FMD virus circulation in domestic livestock in the areas north and west of the detected outbreaks. . (Please see Annex XIII)

01.06.2011 – 25.08.2011 Clinical examinations in susceptible animals in the villages in the risk municipalities of Burgas and Yambol regions as well as in other villages in Burgas, Varna, Shumen, Sliven, Yambol and Haskovo regions.

Further measures taken:

1. Commission Iimplementing Ddecision 2011/493/EU of 5th August 2011 approves plan submitted by Bulgaria to the Commission on 4th April 2011 for the eradication of foot- and mouth disease in wild animals in South-East Bulgaria. The plan has to be implemented within one year period since submission to Commission including certain passive and active surveillance activities in wild life and domestic livestock in an 20 km area along the border with Turkey in South-East Bulgaria defined as “Cordon Sanitaire”. (Please see Annex XIV)

2. Commission Decision 2011/388/EC amended Annex I and II of the Commission Decision 2011/44/EC and reduced regions under restrictions. The restrictive measures laid down with the Decision 2011/44/EC were implemented in the reduced regions listed in Decision 2011/388/EC.The restrictions laid down in Commission Decision 2011/44/EC amended by Commission Decision 2011/388/EC were applied in these areas until 30.09.2011.

3. On 04.07.2011 3 km protective and 10 km surveillance zones imposed according to the Directive 2003/85 were removed the for all outbreaks. It was considered the protective

17 and surveillance zones to be removed later, because of the epidemiological situation of Burgas region, namely: - the fact that the primary source of the FMD outbreaks in Bulgaria remains unclear and the virus could have entered Bulgaria through infected wild boar (or roe deer, der deer) from Turkey - the fact that wild animals could be FMD infected - the type of animal husbandry in the area (extremely extensive, free range), impossible stabling of the animals because of lack of stables in several settlements.

The BFSA took the decision to remove 3 km protective and 10 km surveillance zones around all outbreaks in July on the basis of: - the negative results from the serological examinations and the clinical examinations in domestic animals carried out on 18-19.05.2011 and the end of June showed absence of FMDV in these animals; - the serological examinations in wildlife carried out by the end of June showed absence of FMDV and presence of Ab FMD in small number of wild animals ( 71 wild boar tested – 2 serological positive, 23 roe deer tested – 3 serological positive, 2 red deer – no serological positive by the end of June). c) Vaccines and vaccination. Emergency vaccination as a control measure for FMD has not been implemented on the territory of Bulgaria the country in the context of the outbreak in 2011.

2. FMD surveillance. Surveillance strategy and measures applied in relation with all FMD outbreaks.

Since the confirmtation of the first case of FMD in wild boar in Burgas region the measures for the control and eradication of FMD have been implemented immediately:

2.1 culling of all infected and - where necessary - susceptible animals in the affected villages,

2.2 designation of the risk areas ( 3km protective and and 10km surveillance) and imposing of restrictions in these areas.

A ban of transport and moving animals of susceptible species and products thereof from the restricted areas for the purposes of national consumption and of intra-Community trade were enforced.

According to the Decision 2011/44/EU live animals could only leave the territory of Annex I following a clinical examination direct to the predetermined slaughterhouse in the regions listed in Annex II. They were marked accordingly and their movement recorded in the central database. The animals were handled separately and not come into contact with other animals and products thereof. The meat from animals originating from these animals was marked according to Annex IV to Commission Decision 2011/44/EU and not sent to other Member States but only used for regional consumption.

Since the confirmation of the first FMD outbreak NVS (BFSA) has worked in collaboration with the Police Services in order to ensure avoiding of non legal animal movement on the territory of the regions from Annex I and II to the Decision 2011/44/EU.

18 2.3 clinical examinations and sampling of animals in all villages of the restricted areas.

Daily clinical examinations of the susceptible animals were performed in the villages of the municipalities Tsarevo, Malko Tarnovo, Sredets, Primorsko and Sozopol.

In the villages (nearby the Turkish border and in the villages within the 3 km protection zones and 10 km surveillance zones around the outbreaks all susceptible animals were blood sampled while in all other villages samples were taken according to an estimated 5 % sero- prevalence and 95% confidence. ( please see the Annexes I-XV). Repeated clinical inspections were carried out and sampling has taken place in the course of the epidemic. However, it has also been the case that holdings were inspected and blood sampled twice of even more times negative, before they were found clinically positive for an outbreak of FMD.

During the period 09.01 - 28.02.2011 were carried out serological examinations in the villages located in 3 km protective and 10 km surveillance zones around the outbreaks from the first outbreak wave: IP-1 - in four sampling rounds – per 14 and 7 days. (See Annex I) IP-2 - in four sampling rounds – per 14 and 7 days. (See Annex II) IP-3 - in three sampling rounds – per 7 days. (See Annex III) The next sampling round in these villages was carried out during the time period 16.08- 25.08.2011(see Annex VIII). (All laboratory results are serologically negative)

Serological examinations in the villages located in 3 km protective and 10 km surveillance zones around the outbreaks from the second outbreak wave (IP-4 to IP-11) were carried out in the time period 09.01-25.08.2011 (5 sampling rounds before notification of the outbreak – in 14 and 7 days, (see Annex IV) and 5 sampling rounds after the notification of the outbreak – per 21 and 9 days (see Annexes IX -XIII), as all laboratory results are serological negative.

2.4 Surveillance in East-Balkan pigs 434 blood samples were taken from East-Balkan pigs from 11 herds reared in snoopily municipality which is located noth of the risk area. All tested animals were negative for FMD. The details are shown in annex IV.

2.5 Surveillance in wild animals

Since February till end of August 2011 hunting and trapping of wild susceptible animals was carried out for surveillance purposes along the border with Turkey. During this period in total 127 wild animals were sampled and tested both virologically and serologically. From 89 two wild boar over 1 year of age shot in the area of the first wave of outbreaks in domestic livestock in January, two were serologically positive under Type O ELISA. At the same time 36 roe deer and two red deer were shot and sampled of which 3 roe deer (2 shot near the area of the second wave in March-April and one near the area of the first wave) were Ab positive. No FMDV has been detected.(See annex XIV)

2.6 epidemiological investigations (tracing foreword/tracing backwards)

2.7 public information campaigns targeting mainly animal holders and hunters

19 Special leaflet was distributed among hunters about FMD clinical signs in wild life and the4 sampling procedure.

3.Epidemiological findings and considerations in relation with all FMD outbreaks

So far, the primary source of the FMD outbreaks in Bulgaria remains unclear; the virus could have entered Bulgaria through infected wild boar from Turkey. Alternatively, wild boar or domestic pigs in Bulgaria could have had access to waste food or other means contaminated with FMDV.

The current extent of the infection in the wild boar and wild ruminant population and the duration of a sylvatic FMD epidemic are unknown. Should the entry mechanism be an undetected epidemic in wild boar in Turkish Thrace then introductions may have occurred at any other point along the border or spread silently in the susceptible wildlife population in this region of Bulgaria as described above.

The length of the Bulgarian Turkish border is about 240 km; about half of it being a land and the other half being a river border.

According to the results of the epidemiological investigations it is assumed that Kosti was the first village affected by FMD after the infected wild boar was shot and processed in the village by a farmer during the last two days of 2010 or 1st Jan 2011.

Concerning Rezovo the epidemiology remains unclear how and when the virus reached this village. Full genome analysis indicates that the outbreak in Rezovo is not directly related to the outbreak in Kosti, but may be traced, as the Kosti isolates and three isolate from the wild boar, to a common putative ancestor. There are frequent records of uncontrolled movement of buffalo from Turkey into Bulgaria, including those in October and November 2010. Uncontrolled twenty one buffaloes from Turkish territory came to the village in the beginning of January (4th January) and grazed together with the cattle from the village. The first clinical signs of FMD on the domestic animals originating from the Rezovo village were detected on the 15th January. However, in a young buffalo ( yonger than 12 months age) FMD lesions were observed on the date of killing of the buffalo herd (on 20.01.2011).The FMD NRL confirmed positive for RT-PCR, NSP ELISA and O-type ELISA titre1:256 samples ( on 25.01.2011) from this buffalo taken during the killing . The positive result for RT-PCR was confirmed by the EU Reference Laboratory for FMD in Pirbright on 23.02.2011.

During the killing samples were taken from more 7 buffaloes with positive results for NSP ELISA and ELISA MONO-O-TYPE PRIONICS, confirmed by FMD NRL on 25.01.2011.Data regarding the vaccination against FMD of eight of the 21 buffaloes were obtained from the Turkish side; according to those data those 8 buffaloes were vaccinated in autumn 2010.

The outbreak in Gramatikovo most probably is a result of indirect contact through common grazing ground used previously by infected cattle from Kosti. Only 1 cattle was kept in Gramatikovo village which was grazed in common pasture with infected cattle herds from villige of Kosti. Several small ruminant herds were grazed in the same pasture as well.

So far, the source of the second wave of FMD outbreaks in Sredets municipality (Kirovo, Granichar, Goliamo Bukovo, Fakia, Momina Tsarkva, Dolno Yabalkovo) and Malko

20 Turnovo municipality(Bliznak), remains unclear; one of the hypotheses is that the virus have entered through infected wild boar. Full genome analysis indicates that the outbreaks in that area are not directly related to the outbreak in Kosti or Rezovo, but may be traced, to a common putative ancestor.

However, the full genome sequencing of isolates from the Kirovo outbreak conducted at the EU Reference Laboratory for FMD in Pirbright does not rule out the possibility that there were four incursions of FMD in Bulgaria based on one common ancestor so far.

In conclusion and based on the current epidemiological results available, an EU expert group1 elaborated a timeline (Annex XV attached) leading to the working hypothesis that FMD occurred in wildlife before infection of domestic animals in Kosti. Based on the clinical signs recorded and the laboratory results (antibody and virus tests) it was assumed that the initial infection happened between the 13th and 27th December 2010, although the 1st date could even be earlier than the 13th of December.

The tracebility of the animal movement in Kosti (IP-1) and Rezovo (IP-2) villages, Tsarevo municipality showed that no animals left villages in the time period Nov 2010-09th of Jan 2011. Bovines were kept in free range system without milking, small ruminants were pregnant and in dry milk period in these villages.The animals in the villages in Burgas region were reared and slaughtered for own consumption, so there is no movement of milk or meat in the villages. Furthermore there are no slaughterhouses in Tsarevo municipality which precludes the movement of animals for slaughtering.

There are two slaughterhouses in Burgas region and the made inspections showed that there were no slaughtered animals from Kosti (IP-1), Rezovo (IP-2) and Gramatikovo (IP-3) in these slaughterhouses.

4.Next steps

Clinical examinations and serosurveillance carried out from the last FMD outbreak ( on 07.04.2011) to August 2011 showed lack of FMDV and FMD Ab in domestic animals, lack of FMDV in the wildlife and presence of FMD Ab in small percentage of wildlife.

Commission Iimplementing Ddecision 2011/493/EU of 5th August 2011 approves the plan submitted by Bulgaria to the Commission on 4th April 2011 for the eradication of foot- and- mouth disease in wild animals in South-East Bulgaria. The plan has to be implemented within one year period since submission to Commission including certain passive and active surveillance activities in wild life and domestic livestock in an area in South-East Bulgaria defined as “Cordon Sanitaire” area in South-East Bulgaria

“Cordon Sanitaire” is formed by a defined infected and a neighboring to north and west risk area based on the results of the epidemiological considerations, the geographical distribution of the disease and the unknown FMD situation in the wildlife in Turkish Thrace.

The defined infected area (Area “A”) is a 20 km-wide region in south-east of Bulgaria along the Turkish border with a surface of about 1240 km2. In this area the outbreaks in domestic

1 Between 17 and 21 January 2011 an expert group of the Commission visited Bulgaria (Burgas region) to evaluate the FMD control and eradication measures on the spot. (LINK SCOFCAH)

21 livestock occurred in January and March-April 2011. The area comprises: part of Tsarevo, Malko Tarnovo and south part of Sredets municipalities (Region of Burgas). Within the defined infected area 32 villages are located. The risk area with a surface of about 2160 km2 is divided in two parts (Areas “B” and “C”) and is neighbouring the infected area in the north and west along the Turkish border. These areas have been defined taking into consideration the unclear epidemiological situation of FMD more generally.

The risk area comprises the following municipalities or parts of them: North Tsarevo, Primorsko, Sozopol and North Sredets (Region of Burgas) which form area B and Svilengrad, Topolovgrad (Region of Haskovo), Elhovo, Boliarovo (Region of Yambol) which form area C. Within the defined risk area 74 villages are located.(See Annex XIII )

Control in wildlife

The estimated wild boar population in the defined infected and risk areas together is about 4500 animals. It is assumed that about 1500 wild boar are living in each area (A, B, C). In relation to wild ruminants (Capreolus capreolus and Cervus elaphus spp) the estimated number in the “Cordon Sanitaire” is about 5400. The existing data on population density and size is based on spring counting system.

Serological and virological monitoring of wild life is performed in the defined areas. The sampling strategy in wild boar has been chosen in analogy to the recommendations given for CSF wild boar monitoring, being the only practical guide so far for such purposes. Pursuant to the plan the minimum number of animals to be sampled within a defined sampling area (A, B and C) has to be at least 59 animals to allow detection of 5% sero-prevalence with 95 % confidence. In addition to have a view on the situation in other susceptible wild animals samples for virological and serological testing have to be taken from at least 35 heads of roe deer (Capreolus capreolus), red deer (Cervus elaphus) and other susceptible wild ruminants within a defined sampling area, to allow detection of 10% sero-prevalence with 95 % confidence.

Passive surveillance system is strengthened with the view of an early detecting the viral infection. There is no farmed game on the territory of the defined area “Cordon Sanitare”. Hunters and gamekeepers are instructed to report findings of all dead wild boar or other susceptible wild game (red deer, roe deer) to the veterinary service. Any carcass that is found has to be sampled and laboratory tested.

The animals to be sampled are either be hunted or trapped in wild boar traps (seven large wild boar traps installed along the border with Turkey in the municipalities of Tsarevo and Malko Tarnovo. The location of the traps is shown in Annex XIV).

Control in domestic livestock

Pursuant to the Plan for the eradication of foot and mouth disease in wild animals all 106 villages in the Cordon Sanitaire are visited every 21 days by a team of veterinary experts and all susceptible animals are clinically examined in accordance with Annex III 1 of Directive 2003/85/EC. In case of suspicion samples for laboratory testing (blood and tissue samples) are taken immediately and measures laid down in Directive 2003/85/EC, Articles 4 and 5 applied.

22 In addition to clinical surveillance, routine blood sampling for serological tests is conducted every third month. The minimum number of animals to be sampled in an epidemiological unit will allow detection of 5% sero-prevalence with 95 % confidence. The epidemiological unit is the village. In case of a pure sheep/goat population in the epidemiological unit the blood sampling is carried within the rhythm of the clinical surveillance.

Blood sampling of animals for slaughter is also carried out before the leave the Cordon Sanitaire if they animals are not tested within 21 days period before with a negative result. At the slaughter house a thorough ante and post mortem inspection is be performed for detecting FMD at this stage.

A ban of transport and moving animals of susceptible species and products thereof from the “Cordon Sanitaire” for the purposes of national consumption and of intra-Community trade will be enforced.

Ø Live animals will only leave the “Cordon Sanitaire” following a clinical examination direct to the predetermined slaughterhouse in the region of Burgas, Shuman, Sliven, Yambol and Haskovo (regional consumption). They will be marked accordingly and their movement recorded in the central database. There they will be handled separately and will not come into contact with other animals and products thereof. The meat from animals originating from the Cordon Sanitaire will be marked according to Annex IV to Commission Decision 2011/44/EU and will not be sent to other Member States but only used for regional consumption.

Ø Meats of the bovine, ovine, caprine and porcine species and other bi-ungulates produced from animals of those species in the Cordon Sanitaire will be marked in accordance with the health mark laid down in Annex IV to Decision 2011/44/EU before such meats are used for the regional consumption. No meat products will leave the Cordon Sanitaire unless in compliance with Directive 2002/99/EC.

Ø Milk collected inside the Cordon Sanitaire and dairy products derived from such milk will be treated as laid down in Articles 4 and 5 respectively of Decision 2011/44/EU before they leave this area for regional consumption only. Particular attention at the road posts at the exists of the Cordon Sanitaire will be paid to milk collectors’ means of transport to avoid spill over of FMD from infected to unaffected areas.

Ø Other products of animal origin collected from FMD susceptible domestic species will not be permitted to leave the “Cordon Sanitaire”. In particular, animal by-products will be collected through the Bulgarian rendering system and disposed at the rendering plants at Varna and Shumen or be buried on the spot after treatment with Virkon S or equivalent under the supervision of the representatives of the BGFSA. Hides and skins shall be treated as laid down in Article 8 of Decision 2011/44/EU .

*Please see below the relevant Annexes

23 24 Annex I

SURVEILLANCE IN THE 3km PROTECTION AND 10km SURVEILLANCE ZONES ARROUND THE OUTBREAK IN THE VILLAGE OF KOSTI, TSAREVO MUNICIPALITY (Disease confirmed on 9th Jan) (Outbreak-IP-1)

Fig 10. Location of the settlements in the 10 km surveillance zone around Kosti village, Tsarevo municipality

There are no villages located in the 3 km protection zone. There are 5 villages located in the 10 km surveillance zone of which 4 (Brodilovo, Izgrev, Balgari and Kondolovo) belong to Tsarevo municipality and 1 (Gramatikovo) belongs to Malko Tarnovo municipality.

25 Table 1. First blood sampling of FMD susceptible animals in the settlements in the 10 km surveillance zone around the outbreak in the village Kosti, Tsarevo Municipality (end of sampling 11.01.2011)

Large ruminants sheep goats pigs present Samples taken present Samples taken present Samples taken present Samples taken Settlements № № № № № № № № № № № № № № № № herds animals herds animals herds animals herds animals herds animals herds animals herds animals herds animals 1. Kosti outbreak 4 196 4 132 21 121 18 117 44 205 29 149 8 136 3 21 Settlements in the 10km surveillance zone (Tsarevo Municipality) 4. Brodilovo 3 8 3 5 26 117 26 93 47 164 6 83 3 44 1 18 5. Balgari 0 0 0 0 5 39 5 40 6 12 6 12 0 0 0 0 6. Kondolovo 0 0 0 0 3 48 3 47 1 39 1 37 0 0 0 0 7. Izgrev 0 0 0 0 1 4 0 0 0 0 0 0 1 13 0 0 Settlements in the 10km surveillance zone (Malko Tarnovo municipality) 3. Gramatikovo 1 1 1 1 5 192 2 64 24 111 2 71 0 0 0 0 Total 8 205 8 138 61 521 54 361 122 531 44 352 12 193 4 39 Results: Kosti village: Cattle- 1 positive for NSP ELISA and antibodies FMD type O ELISA Sheep – 14 positive for NSP ELISA and antibodies FMD type O ELISA Goats – 12 positive for NSP ELISA tests and antibodies FMD type O ELISA. Pigs – 8 positive for NSP ELISA.

All other samples are NSP negative .

Table 2. Second blood sampling of FMD susceptible animals in the settlements in the 10 km surveillance zone around the outbreak in the village of Kosti, Tsarevo Municipality (28.01-02.02.2011)

Settlements Large ruminants sheep goats pigs present Samples taken present Samples taken present Samples taken present Samples taken

26 № № № № № № № № № № № № № № № № herds animals herds animals herds animals herds animals herds animals herds animals herds animals herds animals 1. Kosti outbreak 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Settlements in the 10km surveillance zone in Tsarevo Municipality 4. Brodilovo 3 8 3 8 26 117 26 117 47 164 47 164 3 44 3 44 5. Balgari 0 0 0 0 5 39 5 39 6 12 6 12 0 0 0 0 6. Kondolovo 0 0 0 0 3 48 3 48 1 39 1 39 0 0 0 0 7. Izgrev 0 0 0 0 1 4 1 4 0 0 0 0 1 13 1 13 Settlements in the 10km surveillance zone in Malko Tarnovo municipality 3. Gramatikovo 1 1 1 1 5 192 5 192 24 111 24 111 0 0 0 0 Total 4 9 4 9 40 400 40 400 78 326 78 326 4 57 4 57 Note: * Kosti – no samples taken as all susceptible animals in the village were killed and buried by 11.01.11., because detection of FMD outbreak in the village. **The FMD detected in 1 bovine and 13 small ruminant in Gramatikovo village, Malko Tarnovo municipality on 31.01.2011.. Killing and burial of all susceptible animals in the village was carried out on 01.02.2011 All other samples are NSP negative

Table 3. Third blood sampling of FMD susceptible animals in the settlements in the 10 km surveillance zone around the outbreak in the village of Kosti, Tsarevo Municipality (08-09.02.2011)

Large ruminants sheep goats pigs present present present present Settlements Samples taken Samples taken Samples taken Samples taken № № № № № № № № № № № № № № № № herds animals herds animals herds animals herds animals herds animals herds animals herds animals herds animals 1. Kosti outbreak 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Settlements in the 10km surveillance zone in Tsarevo Municipality 4. Brodilovo 3 8 0 0 26 117 2 27 47 164 2 9 3 44 2 23 5. Balgari 0 0 0 0 5 39 1 10 6 12 1 2 0 0 0 0 6. Kondolovo 0 0 0 0 3 48 1 7 1 39 1 28 0 0 0 0 7. Izgrev 0 0 0 0 1 4 0 0 0 0 0 0 1 13 1 9 Settlements in the 10km surveillance zone in Malko Tarnovo municipality 3. Gramatikovo 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

27 Total 3 8 0 0 35 208 4 44 54 215 4 39 4 57 2 32 * Kosti and Gramatikovo – no samples taken as all susceptible animals in the village were killed and buried by 11.01.11 ( Kosti village) and on 01.02.2011 ( Gramatikovo village), because detection of FMD outbreak in the village. All other samples are NSP negative

Table 4. Fourth blood sampling of FMD susceptible animals in the settlements in the 10 km surveillance zone around the outbreak in the village of Kosti, Tsarevo Municipality (23.02-28.02.2011)

Large ruminants sheep goats pigs Samples taken present Samples taken present Samples taken present Samples taken present Settlements № № № № № № № № № № № № № № № № herds animals herds animals herds animals herds animals herds animals herds animals herds animals herds animals 1. Kosti outbreak 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Settlements in the 10km surveillance zone in Tsarevo Municipality 4. Brodilovo 3 8 3 8 1 109 1 109 47 164 47 164 3 44 3 44 5. Balgari 0 0 0 0 5 39 5 39 6 12 6 12 0 0 0 0 6. Kondolovo 0 0 0 0 3 48 3 48 1 39 1 39 0 0 0 0 7. Izgrev 0 0 0 0 1 4 1 4 0 0 0 0 1 13 1 13 Settlements in the 10km surveillance zone in Malko Turnovo Municipality 3.Gramatikovo 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Total 3 8 3 8 10 200 10 200 54 215 54 215 4 57 4 57 All samples are negative for antibody to SP of the FMDV (Mono ELISA O-type Prionics).

28 Annex II SURVEILLANCE IN THE 3km PROTECTION AND 10km SURVEILLANCE ZONES ARROUND THE OUTBREAK IN THE VILLAGE OF REZOVO, TSAREVO MUNICIPALITY (Disease detected clinically on 15th Jan) (Outbreak IP-2)

Fig 11. Location of the settlements in the 10 km surveillance zone around Rezovo village, Tsarevo municipality

In the 3km protection zone there are no settlements. In the 10 km surveillance zone is located only one village – Sinemorets.

29 Table 1. Blood sampling (before the detection of the outbreak) of FMD susceptible animals in the villages of Rezovo and Sinemorets, Tsarevo Municipality (8-11.01.2011)

Large ruminants sheep goats pigs present present present present Settlements Samples taken Samples taken Samples taken Samples taken № № № № № № № № № № № № № № № № herds animals herds animals herds animals herds animals herds animals herds animals herds animals herds animals 2. Rezovo 6 87 6 26 2 68 2 33 2 8 1 3 1 29 0 0 8. Sinemorets 3 14 3 13 9 220 5 120 10 79 3 63 6 9 6 9 Total: 9 101 9 39 11 288 7 153 12 87 4 66 7 38 6 9 The laboratory results: Rezovo: Cattle - 19 samples positive for RT-PCR, 12 positive for Ag ELISA FMD Pigs - 3 positive Ag ELISA FMD All other samples (in Rezovo and Sinemorets) are negative for NSP antibodies to FMD.

Table 2. First blood sampling of FMD susceptible animals in the settlements in the 10 km surveillance zone around the outbreak in the village of Rezovo, Tsarevo Municipality (28.01-02.02.2011)

30 Table 3. Second blood sampling of FMD susceptible animals in the settlements in the 10 km surveillance zone around the outbreak in the village of Rezovo, Tsarevo Municipality (08-09.02.2011)

Large ruminants sheep goats pigs present present present present Settlements Samples taken Samples taken Samples taken Samples taken № № № № № № № № № № № № № № № № herds animals herds animals herds animals herds animals herds animals herds animals herds animals herds animals 2. Rezovo outbreak 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Settlements in the 10km surveillance zone in Tsarevo Municipality 8. Sinemorets 3 14 2 8 9 220 2 71 10 79 1 13 6 9 1 2 Total 3 14 2 8 9 220 2 71 20 79 1 13 6 9 1 2 All samples from Sinemorets: NSP negative.

Table 4. Fourth blood sampling of FMD susceptible animals in the settlements in the 10 km surveillance zone around the outbreak in the village of Rezovo, Tsarevo Municipality ( 23.02.2011-28.02.2011)

31 Annex III

SURVEILLANCE IN THE 3km PROTECTION AND 10km SURVEILLANCE ZONES ARROUND THE OUTBREAK IN THE VILLAGE OF GRAMATIKOVO, MALKO TARNOVO MUNICIPALITY (Disease confirmed on 31st Jan) ( Outbreak IP-3)

Fig 12. Location of the settlements in the 10 km surveillance zone around Gramatikovo, Malko Tarnovo municipality

In the 3 km protection zone is located only one village (Kondolovo, Tsarevo municipality). There are 5 villages located in the 10 km surveillance zone of which 2 (Balgari and Kosti) belong to Tsarevo municipality and 3 (Slivarovo, Zabrenovo and Vizitsa) belong to Malko Tarnovo municipality.

32 Table. 1. First blood sampling of FMD susceptible animals in the settlements in the 3 km protection and 10 km surveillance zones around the outbreak in Gramatikovo, Malko Tarnovo Municipality. (end of sampling 08.02.2011)

Large ruminants sheep goats pigs present Samples taken present Samples taken present Samples taken present Samples taken Settlements № № № № № № № № № № № № № № № № herds animals herds animals herds animals herds animals herds animals herds animals herds animals herds animals 3. Gramatikovo outbreak* 1 1 1 1 5 192 5 192 24 111 24 111 0 0 0 0 Settlements in the 3 km protection zone 4. Kondolovo 0 0 0 0 3 48 3 48 1 39 1 39 0 0 0 0 Settlements in the 10km surveillance zone 1. Slivarovo 0 0 0 0 3 38 3 38 3 48 3 36 3 12 3 12 2. Zabernovo 3 22 2 6 3 9 3 9 12 112 11 73 8 61 3 40 3. Vizitsa 4 57 4 24 5 84 5 55 2 21 2 21 0 0 0 0 5. Balgari 0 0 0 0 5 39 5 39 6 12 6 12 0 0 0 0 6. Kosti** 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Total 8 80 7 31 24 410 24 381 48 343 47 292 11 73 6 52 Note: *Gramatikovo – samples taken during detection of the outbreak on 30.01.2011 and during killing of the animals on 01.02.2011 ** Kosti - all susceptible animals in the village were killed and buried , because detection of FMD outbreak in the village.

The laboratory results Gramatikovo: cattle - 1 samples positive for NS ELISA and FMDAb O type ELISA sheep – 13 positive for NS ELISA and FMDAb O type ELISA. goats – 10 positive for NS ELISA and FMDAb O type ELISA

All samples from the other villages were NSP negative .

Table. 2. Second blood sampling of FMD susceptible animals in the settlements in the 3 km protection and 10 km surveillance zones around the outbreak in Gramatikovo, Malko Tarnovo Municipality (13-16.02.2011)

Settlements Large ruminants sheep goats pigs

33 present Samples taken present Samples taken present Samples taken present Samples taken № № № № № № № № № № № № № № № № herds animals herds animals herds animals herds animals herds animals herds animals herds animals herds animals 3. Gramatikovo outbreak 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Settlements in the 3 km protection zone 4. Kondolovo 0 0 0 0 3 48 1 7 1 39 1 28 0 0 0 0 Settlements in the 10km surveillance zone 1. Slivarovo 0 0 0 0 3 38 3 38 3 48 3 36 3 12 1 7 2. Zabernovo 3 22 3 19 3 9 0 0 12 112 5 67 8 61 2 25 3. Vizitsa 4 57 4 33 5 84 2 81 2 21 1 4 0 0 0 0 5. Balgari 0 0 0 0 5 39 1 10 6 12 1 2 0 0 0 0 6. Kosti 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Total 7 79 7 52 29 218 7 136 24 232 11 137 11 73 3 32 All samples NSP negative.

Table. 3.Third blood sampling of FMD susceptible animals in the settlements in the 3 km protection and 10 km surveillance zones around the outbreak in Gramatikovo, Malko Tarnovo Municipality (23.02.11-28.02.2011) Large ruminants sheep goats pigs present Samples taken present Samples taken present Samples taken present Samples taken Settlements № № № № № № № № № № № № № № № № herds animals herds animals herds animals herds animals herds animals herds animals herds animals herds animals 3. Gramatikovo outbreak 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Settlements in the 3 km protection zone 4. Kondolovo 0 0 0 0 3 43 3 43 1 39 1 39 0 0 0 0 Settlements in the 10km surveillance zone 1. Slivarovo 0 0 0 0 3 38 3 38 3 36 3 36 1 7 1 7 2. Zabernovo 3 22 3 22 3 9 1 4 12 112 12 112 8 61 4 33 3. Vizitsa 4 57 4 10 5 84 4 80 2 21 2 20 0 0 0 0 5. Balgari 0 0 0 0 5 39 5 36 6 12 6 11 0 0 0 0 6. Kosti 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Total 7 77 7 32 29 213 16 201 24 220 24 218 9 68 5 40 All samples negative for antibody to SP of the FMDV (Mono ELISA O-type Prionics).

34 Annex IV SURVEILLANCE IN THE SETTLEMENTS OF BURGAS REGION WHICH ARE LOCATED OUTSIDE THE PROTECTION AND SURVEILLANCE ZONES AROUND THE OUTBREAKS IN JANUARY 2011

(All animals in the villages outside the protection and surveillance zones that are not sampled are clinically checked)

I. SURVEILLANCE IN TSAREVO MUNICIPALITY

Fig13. Location of the settlements in Tsarevo municipality

35 Table 1. First blood sampling of FMD susceptible animals in the settlements of Tsarevo municipality outside of protection and surveillance zones (07- 11.01.2011) Large ruminants sheep goats pigs present Samples taken present Samples taken present Samples taken present Samples taken settlement № № № № № № № № № № № № № № № № herds animals herds animals herds animals herds animals herds animals herds animals herds animals herds animals 3. Ahtopol 11 107 3 53 18 580 3 253 14 116 8 23 3 123 1 2 8. Varvara 4 26 6 27 11 166 8 118 11 52 3 36 0 0 0 0 12. Velika 2 57 3 44 2 28 2 21 4 57 3 35 0 0 0 0 11. Lozenets 0 0 2 22 11 302 8 175 6 181 5 112 1 14 2 6 2. Sinemorets 3 14 4 13 9 220 5 120 10 79 3 63 6 9 6 9 13. Fazanovo 4 174 4 68 1 290 1 54 0 0 0 0 1 25 0 0 10. Tsarevo 8 39 8 25 18 763 15 186 13 31 4 78 2 17 0 0 1. Rezovo 6 87 6 26 2 68 2 33 2 8 1 3 1 29 0 0 Total 38 504 36 278 72 2417 44 960 60 524 27 350 14 217 9 17 All samples NSP negative.

Table 2. Second blood sampling of FMD susceptible animals in the settlements of Tsarevo municipality outside of protection and surveillance zones (28.01-02.02.2011) Large ruminants sheep goats pigs settlement present Samples taken present Samples taken present Samples taken present Samples taken № № № № № № № № № № № № № № № № herds animals herds animals herds animals herds animals herds animals herds animals herds animals herds animals 3. Ahtopol 11 107 11 107 18 580 18 580 14 116 14 116 3 123 3 39 8. Varvara 4 26 4 26 11 166 11 166 11 52 11 52 0 0 0 0 12. Velika 2 57 2 32 2 28 2 21 4 57 1 24 0 0 0 0 11. Lozenets 0 0 0 0 11 302 11 87 6 181 5 56 1 14 1 14 13. Fazanovo 4 174 4 101 1 290 1 56 0 0 0 0 1 25 1 13 10. Tsarevo 8 39 8 39 18 763 18 246 13 31 13 31 2 17 0 0 1. Rezovo* 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Total 29 403 29 305 61 2129 61 1156 48 437 44 279 7 179 5 66 Note: FMD outbreak confirmed in Rezovo village on 15.01.2011 and all susceptible animals in the village were killed and buried by 20.01.2011. All samples NSP negative.

36 Table 3. Third blood sampling of FMD susceptible animals in the settlements of Tsarevo municipality outside protection and surveillance zones (08-09.02.2011) Large ruminants sheep goats pigs present Samples taken present Samples taken present Samples taken present Samples taken settlement № № № № № № № № № № № № № № № № herds animals herds animals herds animals herds animals herds animals herds animals herds animals herds animals 3. Ahtopol 11 107 0 0 18 580 1 50 14 116 3 123 2 30 8. Varvara 4 26 0 0 11 166 3 77 11 52 2 15 0 0 0 0 12. Velika 2 57 1 31 2 28 1 16 4 57 1 16 0 0 0 0 11. Lozenets 0 0 1 7 11 302 1 46 6 181 0 0 1 14 1 14 13. Fazanovo 4 174 1 49 1 290 1 54 0 0 0 0 1 25 1 20 10. Tsarevo 8 39 1 8 18 763 3 124 13 31 3 21 2 17 0 0 1. Rezovo* 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Total 29 403 4 95 61 2129 10 367 48 437 6 52 7 179 4 64 All samples NSP negative.

Table 4. Fourth blood sampling of FMD susceptible animals in the settlements of Tsarevo municipality outside protection and surveillance zones (15-20.02.2011)

Large ruminants sheep goats pigs present Samples taken present Samples taken present present Samples taken settlement № № № № № № № № № № № № № herds animals herds animals herds animals herds animals herds animals herds animals herds 3. Ahtopol 11 107 2 3 18 580 2 80 14 116 1 10 3 123 3 55 8. Varvara 4 26 0 0 11 166 3 71 11 52 2 37 0 0 0 0 12. Velika 2 57 1 25 2 28 1 25 4 57 1 20 0 0 0 0 11. Lozenets 0 0 1 7 11 302 2 67 6 181 1 5 1 14 1 11 13. Fazanovo 4 174 2 52 1 290 1 54 0 0 0 0 1 25 2 32 10. Tsarevo 8 39 1 5 18 763 3 110 13 31 3 25 2 17 1 5 1. Rezovo* 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Total 29 403 7 92 61 2129 12 407 48 437 8 97 7 179 7 103 All samples negative for antibody to SP of the FMDV (Mono ELISA O-type Prionics).

37 Table 5. Fifth blood sampling of FMD susceptible animals in the settlements of Tsarevo municipality outside protection and surveillance zones (26-28.02.2011)

Large ruminants sheep goats pigs present Samples taken present Samples taken present Samples taken present settlement № № № № № № № № № № № № № herds animals herds animals herds animals herds animals herds animals herds animals herds 3. Ahtopol 11 107 0 0 18 580 0 0 14 116 0 0 3 123 0 0 8. Varvara 4 26 0 0 11 166 0 0 11 52 0 0 0 0 0 0 12. Velika 2 57 2 35 2 28 1 20 4 57 1 25 0 0 0 0 11. Lozenets 0 0 0 0 11 302 4 40 6 181 3 60 1 14 0 0 13. Fazanovo 4 174 3 55 1 290 1 54 0 0 0 0 1 25 0 0 10. Tsarevo 8 39 0 0 18 763 0 0 13 31 0 0 2 17 0 0 1. Rezovo* 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Total 29 403 5 90 61 2129 6 114 48 437 4 85 7 179 0 0 All samples negative for antibody to SP of the FMDV (Mono ELISA O-type Prionics).

38 II. SURVEILLANCE IN MALKO TARNOVO MUNICIPALITY

Fig 14. Location of the settlements in Malko Tarnovo municipality

Table 1. First blood sampling of FMD susceptible animals in the settlements of Malko Tarnovo municipality outside of protection and surveillance zones (07-12.01.2011) Large ruminants sheep goats pigs present Samples taken present Samples taken present Samples taken present Samples taken settlement № № № № № № № № № № № № № № № № herds animals herds animals herds animals herds animals herds animals herds animals herds animals herds animals

39 2. Malko Tarnovo 10 102 7 71 37 681 7 309 47 312 4 99 12 118 3 100 13. Bliznak 6 79 3 49 10 82 4 75 7 52 2 35 1 2 1 2 5. Brashlian 3 4 3 4 0 0 0 0 0 0 0 0 0 0 0 0 10. Biala Voda 1 9 1 8 4 32 4 23 2 38 2 43 2 29 1 13 7. Vizitsa 4 57 2 30 5 84 4 83 2 21 2 21 0 0 0 0 12. Evrinozovo 1 10 1 6 1 18 1 14 2 21 2 21 1 27 0 0 6. Zabernovo 3 22 3 24 3 9 1 4 12 112 7 108 8 61 8 58 9. Zvezdets 7 218 3 117 8 116 1 38 6 73 4 62 5 66 1 15 8. Kalovo 0 0 0 0 3 59 2 36 2 51 2 51 3 33 1 8 11. Mladejko 0 0 0 0 0 0 0 0 9 41 9 41 0 0 0 0 1. Slivarovo 0 0 0 0 3 38 3 32 3 48 3 48 3 12 1 9 4. Stoilovo 3 4 3 4 5 76 2 13 14 65 13 48 0 0 0 0 Total 38 505 26 313 79 1195 29 627 106 834 50 577 35 348 16 205 All samples NSP negative.

Table 2. Second blood sampling of FMD susceptible animals in the settlements of Malko Tarnovo municipality outside of protection and surveillance zones (28.01-09.02.2011) Large ruminants sheep goats pigs present Samples taken present Samples taken present Samples taken Samples taken present settlement № № № № № № № № № № № № № № № № herds animals herds animals herds animals herds animals herds animals herds animals herds animals herds animals 2. Malko Tarnovo 10 102 10 81 37 681 23 324 47 312 23 154 12 118 7 105 13. Bliznak 6 79 6 32 10 82 10 59 7 52 7 28 1 2 1 2 5. Brashlian 3 4 2 2 0 0 0 0 0 0 0 0 0 0 0 0 10. Biala Voda 1 9 1 9 4 32 2 20 2 38 2 32 2 29 2 29 12. Evrinozovo 1 10 1 10 1 18 1 14 2 21 1 10 1 27 1 19 9. Zvezdets 7 218 7 119 8 116 2 30 6 73 1 40 5 66 3 43 8. Kalovo 0 0 0 0 3 59 2 59 2 51 1 5 3 33 1 6 11. Mladejko 0 0 0 0 0 0 0 0 9 41 9 41 0 0 0 0 4. Stoilovo 3 4 0 0 5 76 5 61 14 65 6 23 0 0 0 0 Total 31 426 27 253 68 1064 45 567 89 653 50 333 24 275 15 203 All samples NSP negative.

40 Table 3. Third blood sampling of FMD susceptible animals in the settlements of Tsarevo municipality outside protection and surveillance zones (15-20.02.2011) Large ruminants sheep goats pigs present Samples taken present Samples taken present Samples taken present Samples taken settlement № № № № № № № № № № № № № № № № herds animals herds animals herds animals herds animals herds animals herds animals herds animals herds animals 2. Malko Tarnovo 10 102 1 19 37 681 2 55 47 312 2 14 12 118 1 12 13. Bliznak 6 79 0 0 10 82 0 0 7 52 0 0 1 2 0 0 5. Brashlian 3 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 10. Biala Voda 1 9 0 0 4 32 0 0 2 38 0 0 2 29 0 0 12. Evrinozovo 1 10 0 0 1 18 0 0 2 21 0 0 1 27 0 0 9. Zvezdets 7 218 0 0 8 116 1 20 6 73 1 20 5 66 1 15 8. Kalovo 0 0 0 0 3 59 1 17 2 51 1 18 3 33 1 17 11. Mladejko 0 0 0 0 0 0 0 0 9 41 0 0 0 0 0 0 4. Stoilovo 3 4 0 0 5 76 2 32 14 65 1 6 0 0 0 0 Total 31 426 1 19 68 1064 6 131 89 653 5 52 24 275 3 44 All samples are negative for antibody to SP of the FMDV (Mono ELISA O-type Prionics).

Table 4. Fourth blood sampling of FMD susceptible animals in the settlements of Tsarevo municipality outside protection and surveillance zones (26-28.02.2011) settlement Large ruminants sheep goats present Samples taken present Samples taken present Samples taken present Samples taken settlement № № № № № № № № № № № № № № № № herds animals herds animals herds animals herds animals herds animals herds animals herds animals herds animals 2. Malko Tarnovo 10 102 1 7 37 681 9 90 47 312 17 93 12 118 0 0 13. Bliznak 6 79 0 0 10 82 0 0 7 52 0 0 1 2 0 0 5. Brashlian 3 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 10. Biala Voda 1 9 1 9 4 32 2 29 2 38 1 20 2 29 1 13 12. Evrinozovo 1 10 0 0 1 18 0 0 2 21 0 0 1 27 0 0 9. Zvezdets 7 218 2 48 8 116 0 0 6 73 0 0 5 66 0 0 8. Kalovo 0 0 0 0 3 59 2 27 2 51 3 26 3 33 2 10

41 11. Mladejko 0 0 0 0 0 0 0 0 9 41 9 32 0 0 0 0 4. Stoilovo 3 4 0 0 5 76 4 52 14 65 9 46 0 0 0 0 Total 31 426 68 1064 89 653 24 275 All samples are negative for antibody to SP of the FMDV (Mono ELISA O-type Prionics).

III. SURVEILLANCE IN SREDETS MUNICIPALITY

Fig 15. Location of the settlements under surveillance in Sredets municipality

Table 1. First blood sampling of FMD susceptible animals in the settlements of Sredets municipality (07-14.01.2011) settlement Large ruminants sheep goats pigs present Samples taken present Samples taken present Samples taken present Samples taken

42 № № № № № № № № № № № № № № № № herds animals herds animals herds animals herds animals herds animals herds animals herds animals herds animals 1. Belevren 6 136 4 48 0 0 0 0 3 7 3 7 0 0 0 0 2. Bogdanovo 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3. Varovnik 2 204 2 92 0 0 0 0 0 0 0 0 0 0 0 0 4. Valchanovo 2 89 2 33 7 341 7 142 3 158 3 64 0 0 0 0 5. Goliamo Bukovo 7 476 5 90 2 100 2 47 13 57 13 57 1 3 0 0 6. Gorno Yabalkovo 2 80 2 54 6 348 3 216 6 56 3 20 0 0 0 0 7. Granichar 2 229 1 50 0 0 0 0 0 0 0 0 0 0 0 0 8. Dolno Yabalkovo 1 37 1 31 1 26 1 17 3 157 3 115 0 0 0 0 9. Kamene 1 194 1 27 4 313 4 68 1 86 1 16 0 0 0 0 10. Kirovo 2 183 1 37 1 7 1 7 3 30 2 17 0 0 0 0 11. Momina Tsarkva 3 266 3 82 31 2324 31 366 30 142 30 133 0 0 0 0 12. Panchevo 0 0 0 0 2 580 2 56 4 70 4 30 1 14 1 6 13. Slivovo 1 34 1 22 2 154 2 68 8 70 8 67 0 0 0 0 14. Trakiitsi 0 0 0 0 1 9 1 7 2 178 1 47 2 93 1 20 15. Fakia 5 288 4 121 14 421 10 202 40 252 42 239 0 0 0 0 Total 34 2216 27 687 71 4623 64 1196 116 1263 113 812 4 110 2 26 All samples NSP negative.

Table 2. Second blood sampling of FMD susceptible animals in the settlements of Sredets municipality (28.01-03.02.2011) Large ruminants sheep goats pigs present Samples taken present Samples taken present Samples taken present Samples taken settlement № № № № № № № № № № № № № № № № herds animals herds animals herds animals herds animals herds animals herds animals herds animals herds animals 1. Belevren 6 136 6 125 0 0 0 0 3 7 3 7 0 0 0 0 2. Bogdanovo 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3. Varovnik 2 204 0 0 0 0 0 0 0 0 0 0 0 0 0 0 4. Valchanovo 2 89 0 0 7 341 0 0 3 158 0 0 0 0 0 0

43 5. Goliamo Bukovo 7 476 4 188 2 100 2 55 13 57 12 52 1 3 0 0 6. Gorno Yabalkovo 2 80 2 73 6 348 6 348 6 56 6 56 0 0 0 0 7. Granichar 2 229 2 154 0 0 0 0 0 0 0 0 0 0 0 0 8. Dolno Yabalkovo 1 37 1 37 1 26 1 22 3 157 3 108 0 0 0 0 9. Kamene 1 194 0 0 4 313 0 0 1 86 0 0 0 0 0 0 10. Kirovo 2 183 2 95 1 7 1 7 3 30 2 17 0 0 0 0 11. Momina Tsarkva 3 266 3 95 31 2324 31 387 30 142 30 139 0 0 0 0 12. Panchevo 0 0 0 0 2 580 0 0 4 70 0 0 1 14 0 0 13. Slivovo 1 34 0 0 2 154 0 0 8 70 0 0 0 0 0 0 14. Trakiitsi 0 0 0 0 1 9 1 9 2 178 1 47 2 93 1 23 15. Fakia 5 288 0 168 14 421 0 212 40 252 0 243 0 0 0 0 Total 34 2216 20 767 71 4623 42 828 116 1263 57 426 4 110 1 23 All samples NSP negative.

Table 3. Third blood sampling of FMD susceptible animals in the settlements of Sredets municipality (07-10.02.2011). Large ruminants sheep goats pigs present Samples taken present Samples taken present Samples taken present Samples taken settlement № № № № № № № № № № № № № № № № herds animals herds animals herds animals herds animals herds animals herds animals herds animals herds animals 1. Belevren 6 136 2 54 0 0 0 0 3 7 0 0 0 0 0 0 2. Bogdanovo 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3. Varovnik 2 204 0 0 0 0 0 0 0 0 0 0 0 0 0 0 4. Valchanovo 2 89 0 0 7 341 0 0 3 158 0 0 0 0 0 0 5. Goliamo Bukovo 7 476 3 121 2 100 0 0 13 57 0 0 1 3 1 3 6. Gorno Yabalkovo 2 80 1 38 6 348 2 93 6 56 1 3 0 0 0 0 7. Granichar 2 229 1 48 0 0 0 0 0 0 0 0 0 0 0 0 8. Dolno Yabalkovo 1 37 1 31 1 26 1 25 3 157 1 20 0 0 0 0 9. Kamene 1 194 0 0 4 313 0 0 1 86 0 0 0 0 0 0 10. Kirovo 2 183 1 47 1 7 0 0 3 30 1 13 0 0 0 0 11. Momina Tsarkva 3 266 1 45 31 2324 1 57 30 142 0 0 0 0 0 0 12. Panchevo 0 0 0 0 2 580 0 0 4 70 0 0 1 14 0 0

44 13. Slivovo 1 34 0 0 2 154 0 0 8 70 0 0 0 0 14. Trakiitsi 0 0 0 0 1 9 0 0 2 178 1 43 2 93 1 24 15. Fakia 5 288 0 0 14 421 1 53 40 252 0 0 0 0 0 0 Total 34 2216 10 384 71 4623 5 228 116 1263 4 79 4 110 2 27 All samples NSP negative.

Table 4. Fourth blood sampling of FMD susceptible animals in the settlements of Sredets municipality (16-20.02.2011). Large ruminants sheep goats pigs present Samples taken present Samples taken present Samples taken present Samples taken settlement № № № № № № № № № № № № № № № № herds animals herds animals herds animals herds animals herds animals herds animals herds animals herds animals 1. Belevren 6 136 6 70 0 0 0 0 3 7 3 7 0 0 0 0 2. Bogdanovo 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3. Varovnik 2 204 0 0 0 0 0 0 0 0 0 0 0 0 0 0 4. Valchanovo 2 89 2 33 7 341 7 142 3 158 3 64 0 0 0 0 5. Goliamo Bukovo 7 476 3 121 2 100 0 0 13 57 0 0 1 3 1 3 6. Gorno Yabalkovo 2 80 1 38 6 348 0 90 6 56 0 0 0 0 0 0 7. Granichar 2 229 1 48 0 0 0 0 0 0 0 0 0 0 0 0 8. Dolno Yabalkovo 1 37 1 31 1 26 1 24 3 157 1 19 0 0 0 0 9. Kamene 1 194 0 0 4 313 0 0 1 86 0 0 0 0 0 0 10. Kirovo 2 183 1 48 1 7 0 0 3 30 1 13 0 0 0 0 11. Momina Tsarkva 3 266 0 51 31 2324 0 57 30 142 0 0 0 0 0 0 12. Panchevo 0 0 0 0 2 580 0 0 4 70 0 0 1 14 0 0 13. Slivovo 1 34 1 22 2 154 2 68 8 70 65 0 0 0 14. Trakiitsi 0 0 0 0 1 9 0 0 2 178 1 43 2 93 1 24 15. Fakia 5 288 0 0 14 421 1 53 40 252 0 0 0 0 0 0 Total 34 2216 16 462 71 4623 11 366 116 1263 68 139 4 110 2 27 All samples are negative for antibody to SP of the FMDV (Mono ELISA O-type Prionics).

Table 5. Fifth blood sampling of FMD susceptible animals in the settlements of Sredets municipality (25-28.02.2011).

settlement Large ruminants sheep goats pigs present Samples taken present Samples taken present Samples taken present Samples taken

45 № № № № № № № № № № № № № № № № herds animals herds animals herds animals herds animals herds animals herds animals herds animals herds animals 1. Belevren 6 136 2 71 0 0 0 0 3 7 1 7 0 0 0 0 2. Bogdanovo 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3. Varovnik 2 204 0 90 0 0 0 0 0 0 0 0 0 0 0 0 4. Valchanovo 2 89 0 0 7 341 0 0 3 158 0 0 0 0 0 0 5. Goliamo Bukovo 7 476 3 142 2 100 2 55 13 57 13 47 1 3 1 3 6. Gorno Yabalkovo 2 80 1 38 6 348 2 93 6 56 1 3 0 0 0 0 7. Granichar 2 229 1 48 0 0 0 0 0 0 0 0 0 0 0 0 8. Dolno Yabalkovo 1 37 1 31 1 26 1 25 3 157 1 111 0 0 0 0 9. Kamene 1 194 0 0 4 313 0 0 1 86 0 0 0 0 0 0 10. Kirovo 2 183 1 48 1 7 0 7 3 30 1 17 0 0 0 0 11. Momina Tsarkva 3 266 0 90 31 2324 0 383 30 142 0 124 0 0 0 0 12. Panchevo 0 0 0 0 2 580 0 0 4 70 0 0 1 14 0 0 13. Slivovo 1 34 0 0 2 154 0 0 8 70 0 0 0 0 14. Trakiitsi 0 0 0 0 1 9 0 8 2 178 1 29 2 93 1 24 15. Fakia 5 288 5 164 14 421 1 216 40 252 40 237 0 0 0 0 Total 34 2216 9 722 71 4623 6 787 116 1263 45 451 4 110 2 27 All samples are negative for antibody to SP of the FMDV (Mono ELISA O-type Prionics).

46 IV. SURVEILLANCE IN PRIMORSKO MUNICIPALITY

Fig 16. Location of the settlements under surveillance in Primorsko municipality

Table 1. First blood sampling of FMD susceptible animals in the settlements of Primorsko municipality (12-14.01.2011) Large ruminants sheep goats pigs present Samples taken present Samples taken present Samples taken present Samples taken settlement № № № № № № № № № № № № № № № № animal herds animals herds animals herds herds animals herds animals herds animals herds animals herds animals s 1. Kiten 6 121 3 21 19 298 11 45 17 93 10 21 2 6 0 0 2. Pismenovo 1 14 2 6 5 206 5 91 4 17 2 13 2 13 2 9 3. Primorsko 11 112 0 0 12 472 11 63 17 96 8 9 8 128 0 0 4. Yasna Poliana 4 57 0 0 21 2074 5 124 29 187 0 0 19 160 0 0 5. Veselie 8 174 0 0 36 4702 0 0 12 78 0 0 9 43 0 0

47 6. Novo Panicharevo 6 97 0 0 22 2647 0 0 43 242 0 0 22 129 0 0 Total 36 575 5 27 115 10399 32 323 122 713 20 43 62 479 2 9 All samples NSP negative.

Table 2. Second blood sampling of FMD susceptible animals in the settlements of Primorsko municipality (03.02-05.02.2011) Large ruminants sheep goats pigs present Samples taken present Samples taken present Samples taken present Samples taken settlement № № № № № № № № № № № № № № № № herds animals herds animals herds animals herds animals herds animals herds animals herds animals herds animals 1. Kiten 6 121 5 66 19 298 19 104 17 93 14 42 2 6 2 4 2. Pismenovo 1 14 1 14 5 206 5 206 4 17 4 17 2 13 2 9 3. Primorsko 11 112 11 92 12 472 12 171 17 96 15 71 8 128 1 3 4. Yasna Poliana 4 57 0 0 21 2074 0 0 29 187 0 0 19 160 0 0 5. Veselie 8 174 0 0 36 4702 0 0 12 78 0 0 9 43 0 0 6. Novo Panicharevo 6 97 0 0 22 2647 0 0 43 242 0 0 22 129 0 0 Total 36 575 17 172 115 10399 36 481 122 713 33 130 62 479 5 16

All samples NSP negative.

V. SURVEILLANCE IN SOZOPOL MUNICIPALITY

48 Fig.17. Location of the settlements under surveillance in Sozopol municipality Table 1. First blood sampling of FMD susceptible animals in the settlements of Sozopol municipality (14.01.2011) Large ruminants sheep goats pigs present Samples taken present Samples taken present Samples taken present Samples taken Settlement № № № № № № № № № № № № № № № № herds animals herds animals herds animals herds animals herds animals herds animals herds animals herds animals 1. Indje Voyvoda 0 0 0 0 0 0 0 0 0 0 0 0 1 72 1 22 2. Varshilo 0 0 0 0 0 0 0 0 0 0 0 0 5 777 0 0 3. Krushovets 0 0 0 0 0 0 0 0 0 0 0 0 8 811 2 53 Total 0 0 0 0 0 0 0 0 0 0 0 0 14 1660 3 75 All samples NSP negative.

Table 2. Second blood sampling of FMD susceptible animals in the settlements of Sozopol municipality (07.02.2011) Large ruminants sheep goats pigs present Samples taken present Samples taken present Samples taken present Samples taken Settlement № № № № № № № № № № № № № № № № herds animals herds animals herds animals herds animals herds animals herds animals herds animals herds animals 1. Indje 0 0 0 0 0 0 0 0 0 0 0 0 1 72 1 40

49 Voyvoda 2. Varshilo 0 0 0 0 0 0 0 0 0 0 0 0 5 777 5 194 3. Krushovets 0 0 0 0 0 0 0 0 0 0 0 0 8 811 8 200 Total 0 0 0 0 0 0 0 0 0 0 0 0 14 1660 14 434 All samples NSP negative.

FMD surveillance in East-Balkan pig herds

Table: East-Balkan pigs herds and samples taken on 07.02.2011 № Holding Number Settlement Owners blood identification of pigs samples Indze Voyvoda S A D 81490097 72 40 G P S 81480001 120 48 V Z Y 81480185 250 48 Krushevets G K 81480012 16 16 P H D 81480217 60 38 I H D 81480218 75 50 Varshilo K D Y 81570100 300 54 D G F 81570058 25 20 H D A 81570023 30 16

50 № Holding Number Settlement Owners blood identification of pigs samples Indze Voyvoda S A D 81490097 72 40 G P S 81480001 120 48 V Z Y 81480185 250 48 Krushevets G K 81480012 16 16 P H D 81480217 60 38 I H D 81480218 75 50 K D Y 81570100 300 54 D G F 81570058 25 20 Varshilo H D A 81570023 30 16 R S Z 81570078 180 52 D I D 81570080 220 52

Total 1348 434

Fig.18 Location of the 11 East-Balkan pigs herds in Sozopol municipality

All samples NSP negative

51 Annex V

SURVEILLANCE IN THE 3km PROTECTION AND 10km SURVEILLANCE ZONES ARROUND THE OUTBREAK IN THE VILLAGE OF KIROVO, SREDETS MUNICIPALITY (Disease confirmed on19th of March) ( Outbreak IP-4)

Fig 20. Location of the infected farm (№IV outbreak-Kirovo) and the distance from the last outbreak (№ 3 outbreak –Gramatikovo)

In the 3 km protection zone are located two villages (Kirovo and Dolno Yabalkovo, Tsarevo municipality). There are 7 villages located in the 10 km surveillance zone Belevren, Granichar, Golyamo Bukovo, Trakiitsi, Varovnik, Gorno Yabalkovo and Fakia, Sredets municipality).

Table 1. Blood sampling of FMD susceptible animals in the settlements in the 3 km protection and 10 km surveillance zones around the outbreak in Kirovo, Sredets Municipality (18.03.11-30.03.2011)

52 Large ruminants sheep goats pigs

present present present Samples taken Samples taken settlement Samples taken Samples taken present № № № № № № № № № № № № № № № № animal anima anima herds animals herds herds animals herds herds herds animals herds herds herds animals s ls ls Kirovo- 1 143 1 42 0 0 0 0 0 0 0 0 0 0 0 0 bovine farm

Settlements in the 3 km protection zone

1Kirovo 1 135 1 52 1 7 1 7 2 17 2 4 0 0 0 0 2.Dolno Yabalkovo 1 45 1 31 1 69 1 3 1 287 3 45 1 2 1 6 Settlements in the 10 km surveillance zone 1. Belevren 1 65 1 17 0 0 0 0 3 7 0 0 0 0 0 0 2. Granichar 1 133 1 15 0 0 0 0 0 0 0 0 0 0 0 0 3. Golyamo Bukovo 5 319 5 119 2 97 2 97 1 57 1 51 1 11 1 3 4. Trakiici 0 0 0 1 8 1 5 1 94 1 35 2 88 2 10 5. Varovnik 1 216 1 50 0 0 0 0 0 0 0 0 0 0 0 0 6.Gorno Yabalkovo 2 77 0 o 6 348 0 0 6 56 0 0 0 0 0 0 7.Fakia 5 278 5 220 4 469 4 132 2 288 2 59 0 0 0 0 Total 18 1411 16 546 15 998 9 244 16 806 9 194 4 101 4 19 Laboratory results: Kirovo bovine farm – 15 samples from cattle positive for RT PCR, 28 samples positive for Ab ELISA All samples are negative for antibody to SP of the FMDV (Mono ELISA O-type Prionics).

53 Annex VI

SURVEILLANCE IN THE 3km PROTECTION AND 10km SURVEILLANCE ZONES ARROUND THE OUTBREAK IN THE VILLAGE OF GRANICHAR , SREDETS MUNICIPALITY (Disease confirmed on 24th of March) ( Outbreak IP-5)

Fig 21. Location of the settlements in the 3km protective and 10 km surveillance zones around Granitchar village, Sredets municipality

In the 3 km protection zone are located two villages (Bogdanovo and Dolno Yabalkovo, Tsarevo municipality). There are 8 villages located in the 10 km surveillance zone Belevren, Kirovo, Golyamo Bukovo, Trakiitsi, Varovnik, Sredets municipality , Evrenozovo, Bliznak and Madezhko, Malko Turnovo municipality).

Table 1. Blood sampling of FMD susceptible animals in the settlements in the 3 km protection and 10 km surveillance zones around the outbreak in Granitchar, Sredets Municipality (18.03.11-30.03.2011)

54 Large ruminants sheep goats pigs present Samples taken present Samples taken present Samples taken present Samples taken Settlements № № № № № № № № № № № № № № № № anima animal anima herds animals herds herds animals herds animals herds herds herds animals herds animals ls s ls 1 Granichar – farm (IP 5) 1 133 1 15 0 0 0 0 0 0 0 0 0 0 0 0 Settlements in the 3 km protection zone Bogdanovo 2 374 0 0 3 175 0 0 2 121 0 0 1 41 0 0 Dolno Yabalkovo 1 45 1 31 1 356 1 5 3 45 1 2 1 6 Settlements in the 10 km surveillance zone 1. belevren 1 65 1 17 0 0 0 0 3 7 0 0 0 0 0 0 2.Kirovo 1 135 1 52 1 7 1 7 2 17 2 4 0 0 0 0 3. Golyamo Bukovo 5 319 5 119 2 97 2 97 1 57 1 51 1 11 1 4 4. Trakiici 0 0 0 0 1 8 1 5 1 94 1 35 2 88 2 10 5. Varovnik 1 216 1 50 0 0 0 0 0 0 0 0 0 0 0 0 6.evrenezovo 1 10 1 18 1 14 1 14 1 10 1 10 1 23 1 10 1. Bliznak 5 137 5 96 6 158 6 121 6 111 6 91 2 9 2 6 8.Mladejko 0 0 0 0 0 0 0 0 9 33 9 33 0 0 0 0 Total 18 1434 16 398 15 815 12 249 25 450 23 269 8 174 7 36 Laboratory results: Granichar farm – 11 samples from cattle positive for RT PCR, 15 samples positive for ELISA O type All samples are negative for antibody to SP of the FMDV (Mono ELISA O-type Prionics).

55 Annex VII

SURVEILLANCE IN THE 3km PROTECTION AND 10km SURVEILLANCE ZONES ARROUND THE OUTBREAKS IN THE VILLAGE OF GOLIAMO BUKOVO, FAKIA, MOMINA TSURKVA, SREDETS MUNICIPALITY (Disease confirmed on 25th of March in outbreaks IP-6, IP-7, IP-8,)

Fig 22. Location of the three outbreaks ( IP-6, IP-7, IP-8) in Sredets municipality, Burgas region with the 3 km protection and 10 km surveillance zones around the last outbreaks.

GOLIAMO BUKOVO outbreak IP-6 - In the 3 km protection zone are located three villages (Golyamo Bukovo, Kirovo and Trakiitsi, Tsarevo municipality). There are 6 villages located in the 10 km surveillance zone (Bliznak, Granitchar, Fakia, Varovnik, Dolno Yabulkovo, Bogdanovo, Sredets municipality).

56 FAKIA outbreak IP-7 - In the 3 km protection zone is located one village (Fakia, Tsarevo municipality). There are 6 villages located in the 10 km surveillance zone (Gorno Yabalkovo, Dolno Ybalkovo, Kirovo, Golyamo Bukovo, Slivovo, Momina Tsurkva, Sredets municipality)

MOMINA TSURKVA outbreak IP-8 -in the 3 km protection zone is located one village (Momina Tsurkva, Tsarevo municipality). There are 7 villages located in the 10 km surveillance zone (Gorno Yabalkovo, Dolno Ybalkovo, Fakia, Sredets municipality, Burgas region, Golyamo Krushevo, Vulchi izvor, Gorska Polyana, Strandzha, Bolyarovo municipality, Yambol region)

Table 1. Blood sampling of FMD susceptible animals in the settlements in the 3 km protection and 10 km surveillance zones around the outbreak in Goliamo Bukovo, Fakia, Momina Tsurkva, Sredets Municipality (18.03.11-30.03.2011)

Large ruminants sheep goats pigs

Samples taken Samples taken Samples taken Samples taken Settlements Present Present Present Present № № № № № № № № № № № № № № № № anim anim herds animals herds animals herds animals herds animals herds herds herds animals herds animals als als 6 farm- Golyamo Bukovo (IP 6) 2 188 2 57 1 11 1 3 Fakia- farm (IP 7) 1 81 1 81 0 0 0 0 0 0 0 0 0 0 0 0 Momina Tsurkva outbreak (IP 8) 1 209 1 11 Settlements in 3km protective zones around the three outbreaks Golyamo Bukovo 3 131 3 62 2 97 2 97 1 57 1 51 Kirovo 1 135 1 52 1 7 1 7 2 17 2 4 0 0 0 0 Trakiici 0 0 0 0 1 8 1 5 1 94 1 35 2 88 2 10 Fakia 4 197 4 122 4 469 4 132 2 288 2 59 0 0 0 0 7.Momina carkva 3 133 2 53 5 2194 5 287 3 161 3 60 1 57 1 10 Settlements in 10km surveillance zones around the three outbreaks 1. Bliznak 5 93 5 71 6 89 6 62 6 92 6 36 2 9 2 6 2. Granichar 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 4. Varovnik 1 216 1 50 0 0 0 0 0 0 0 0 0 0 0 0

57 5.Dolno Yabalkovo 1 45 1 31 1 85 1 26 4 279 4 80 1 6 1 4 6.Bogdanovo 2 374 2 299 3 254 3 175 2 121 2 121 1 41 1 41 1. Gorno Yabalkovo 0 0 2 77 6 348 2 56 0 0 0 0 3. Kirovo 1 135 1 52 1 7 1 7 2 17 2 4 0 0 0 0 6.Slivovo 1 31 1 29 2 154 1 70 0 0 0 0 0 0 4. Golyamo Krushevo 0 0 0 0 10 504 1 28 20 305 15 28 0 0 0 0 5. valchi izvor 0 0 0 0 1 45 1 20 6 80 1 23 0 0 0 0 6.Gorska Polyana 2 109 2 25 6 60 2 21 10 80 1 23 0 0 0 0 7.Strandzha 4 407 2 28 5 80 2 27 4 50 1 6 0 0 0 0 Total 32 2072 31 1512 54 4398 30 897 66 1695 41 602 8 126 8 160 Note: * Granichar - all susceptible animals in the village were killed and buried by 26.03.2011, because detection of FMD outbreak in the village on 24.03.2011. The laboratory results are:

Goliamo Bukovo – Cattle:11 samples positive for RT-PCR , 3 of them positive for FMD Ag ELISA, all of them negative for Mono - O type ELISA Prionics

Fakia Cattle- 6 positive for RT-PCR and 1 sample positive for FMD Ag ELISA

Momina Tsarkva cattle – 6 positive for RT-PCR, 2 positive for FMD Ag ELISA. All other samples are negative for antibody to SP of the FMDV (Mono ELISA O-type Prionics).

58 Annex VIII SURVEILLANCE IN THE 3km PROTECTION AND 10km SURVEILLANCE ZONES ARROUND THE OUTBREAKS IN THE VILLAGE OF BLIZNAK , MALKO TURNOVO MUNICIPALITY (Disease confirmed on 3rd of April) ( Outbreaks IP-9 and IP-10)

1 2

)Fig 23. Location of the settlements in the 3km protective and 10 km surveillance zones around the outbreaks ( №IX and X) in Bliznk village, Malko Turnovo municipality

In the 3 km protection zone there are no villages.There are 5 villages located in the 10 km surveillance zone (Evrenozovo, Mladezhko and Zvezdets, Malko Turnovo municipality, Bogdanovo, Varovnik, Trakiitsi and Granitchar, Sredets municipality, Burgas region

Table 1. Blood sampling of FMD susceptible animals in the settlements in the 3 km protection and 10 km surveillance zones around the outbreaks in Bliznak, Sredets Municipality (18.03.11-30.03.2011). Settlements Large ruminants sheep goats pigs Samples Samples taken Samples taken Samples taken Present Present Present Present taken

59 № № № № № № № № № № № № № № № № ani animal anima herds animals herds animals herds animals herds animals herds animals herds herds herds mal s ls s Bliznak village (IP 9) 4 21 5 21 5 69 5 42 5 42 5 36 2 9 2 6

Bliznak buffalo herd (IP 10 1 72 1 57 1 20 1 20 1 52 1 36 0 0 0 0 Settlements in 10km surveillance zones around the three outbreaks Evrenozovo 1 24 1 12 1 51 1 14 1 39 1 10 1 39 1 23 Mladezhko 0 0 0 0 0 0 0 0 9 97 9 33 0 0 0 0 Zvezdets 6 297 6 0 9 187 9 106 3 94 3 63 3 44 3 44 Bogdanovo 2 374 0 0 3 175 3 175 2 121 2 121 1 41 0 0 Varovnik 1 233 1 0 0 0 0 0 0 0 0 0 0 0 Granichar 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Trakiitsi 0 0 0 0 1 8 1 5 1 94 1 35 2 88 2 10 Total 15 1021 14 90 20 589 20 362 22 550 22 334 9 221 8 83

The laboratory results are as follow: Bliznak village Cattle - 21 samples positive for FMD ELISA Type O Priocheck and FMD NS ELISA Sheep - 42 samples positive for FMD ELISA Type O Priocheck and FMD NS ELISA Goats 36 samples positive for FMD ELISA Type O Priocheck and FMD NS ELISA Buffalo Farm Buffaloes – 56 samples positive for FMD ELISA Type O Priocheck , 43 positive for FMD NS ELISA Sheep - 20 samples positive for FMD ELISA Type O Priocheck and FMD NS ELISA Goats - 36 positive for FMD ELISA Type O Priocheck and FMD NS ELISA. All other samples are negative for antibody to SP of the FMDV (Mono ELISA O-type Prionics) and FMD NS ELISA

60 Annex IX SURVEILLANCE IN THE 3km PROTECTION AND 10km SURVEILLANCE ZONES ARROUND THE OUTBREAK IN THE VILLAGE OF DOLNO YABULKOVO, SREDETS MUNICIPALITY (Disease confirmed on 7th of April) (Outbreak IP-11)

Fig24. Location of the settlements in the 3km protective and 10 km surveillance zones around Dolno Yabalkovo village, Sredets municipality

In the 3 km protection zone is located one village (Gorno Yabalkovo, Tsarevo municipality). There are 6 villages located in the 10 km surveillance zone (Momina Tsurkva, Fakia, Golyamo Bukovo, Kirovo, Belevren, Granitchar, Sredets municipality, Burgas region)

61 Table 1. Blood sampling of FMD susceptible animals in the settlements in the 3 km protection and 10 km surveillance zones around the outbreaks in Bliznak, Sredets Municipality (07.04.11-15.04.2011)

Large ruminants sheep goats pigs Present Samples taken Present Samples taken Present Samples taken Present Samples taken Settlement № № № s № № № № № № № № № № № № № ani herd anima herds animals herds animals herds animals herds animals herds animals animals herds herds mal s ls s Dolno Yabulkov 1 45 1 35 1 85 1 26 4 279 4 80 1 6 1 4 o Settlements in 3km protective zones around the outbreak Gorno Yabalkov o 2 129 2 77 6 546 6 348 2 87 2 52 0 0 0 0 Settlements in 10km surveillance zones around the outbreak 2. Momina Tsurkva 2 85 2 57 5 2194 5 752 3 232 3 161 1 57 0 0 3. Fakia 4 197 4 118 4 132 4 83 2 59 2 32 0 0 0 0 4. Golyamo Bukovo 3 131 3 102 2 97 2 90 1 57 1 51 0 0 0 0 5. Kirovo 1 135 1 135 1 7 1 7 2 17 2 10 0 0 0 0 6.Belevre n 1 65 1 65 0 0 0 0 3 7 3 7 0 0 0 0 7.Granich ar 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Total 14 787 14 532 21 3061 19 1306 17 738 17 393 2 63 1 4

The laboratory results are as follow: Dolno Yabulkovo: Cattle -2 positive for Mono ELISA O-type Prionics. Goats/sheep -22 positive for Mono ELISA O-type Prionics All samples are negative for antibody to SP of the FMDV (Mono ELISA O-type Prionics)

62 Annex X Surveillance in domestic livestock (18.04-19.04.2011)

I. Clinical examinations and serological surveillance of susceptible animals in the settlements located in 3 km protective and 10 km surveillance zones around the outbreaks of the second wav e(March –April)

Fig 25. Settlements located in 3 km protective and 10 km surveillance zones around the second outbreak wave.

Table 1. Blood sampling in the susceptible animals in the settlements located in 3 km protective and 10 km surveillance zones around the outbreaks ( second outbreak wave)

63 Animal № of Animals in the settlements holdings blood samples № of taken to № of Total blood

) detect 5 % blood Municipalities Settlements number of samples № prevalence samples animals taken per with 95% taken

Pigs animals goats cattle Sheep

Buffalo confidence

number ( number within village

Settlements in 3 km protective zones around the outbreaks IT- 4 to IT-11 bovine-48 Kirovo 4 135 7 17 0 0 159 49 72 sheep -7 bovine-54goats-17 Golyamo 14 134 94 51 0 0 319 54 103 sheep-42 Bukovo goats-7 bovine- 128 Fakiya 64 180 469 288 38 0 975 57 302 sheep-67 goats-107 sheep-7 Trakiitsi 4 8 94 0 88 180 50 67 goats-46 Sredets pigs-14 municipality, Burgas region Granichar* 0 0 0 0 0 0 0 0 0 0

bovine-35 Gorno 10 77 348 52 0 0 477 55 225 sheep-173 Ybulkovo goats-17 Dolno 0 0 0 0 0 0 0 0 0 0 Yabalkovo*

bovine-11 Momina 66 57 2194 161 0 57 2412 58 274 sheep-237 Tsurkva goats-26

Malko Bliznak* 0 0 0 0 0 0 0 0 0 0 Turnovo

64 goats- 10,sheep- Evrenozovo 2 12 14 10 0 23 59 38 44 11,bovine- 4,pigs-19

settlements in 10 km surveillance zones the outbreaks IT- 4 to IT-11 bovine- 102 Bogdanovo 22 375 175 121 0 41 671 57 307 sheep-132 goats-73 bovine-90 Varovnik 2 216 0 0 0 0 216 51 90 bovine-53 goats-6 Sredets bovine-90 municipality, Belevren 4 74 0 7 0 0 81 42 59 bovine-53 Burgas region goats-6 bovine-12 Vulchanovo 12 118 199 157 0 0 474 55 60 sheep-33 goats-15

bovine-26 Slivovo 8 31 158 70 0 0 259 53 132 sheep-87 goats-19

Zvezdets 15 146 106 63 79 44 453 56 72 bovine-72 Malko Turnovo municipality, Burgas region Mladezhko 9 0 0 40 0 0 40 31 32 goats-32

Golyamo sheep-28 30 0 504 305 0 0 809 57 58 krushevo goats-30

bovine--28 Bolyarovo Gorska 18 109 60 80 0 0 249 53 72 sheep-21 municipality, polyana Burgas region goats-23

bovine--28 Strandzha 13 407 80 50 0 0 537 56 61 sheep-27 goats-6

65 20 Total 297 2071 4416 1566 117 253 8370 872 2030 villages

Note – no susceptible animals in Granichar, Bliznak, Dolno Yabalkovo because detection of FMD outbreak in these villages ( outbreaks IP-5, IP-10 and IP 11) All samples are negative for antibody to SP of the FMDV (Mono ELISA O-type Prionics)

II. Serological surveillance in the villages in the risk municipalities of Burgas and Yambol regions as well as in other villages in Burgas, Varna, Shumen, Sliven, Yambol and Haskovo regions for proving absence of FMD virus circulation in domestic livestock in the areas north and west of the detected outbreaks

66 FMD case in wild boar First FMD outbreak wave Second FMD outbreak wave Settlements where the blood sampling in the susceptible animals were carried out Fig 26 -Blood sampling in the susceptible animals in the settlements located in the regions outside and around the 3 km protective and 10 km surveillance zones around the second outbreak wave included in Annexes I of Decision2011/44/ЕС.

Table 2 – Blood sampling in the susceptible animals in the settlements located in the regions outside and around the 3 km protective and 10 km surveillance zones around the second outbreak wave included in Annexes I of Decision2011/44/ЕС.

Total № of № of East Balkan Animal № of blood samples Municipalities Settlements Animals in the settlements number blood blood pigs holdings taken per animals of samples samples

67 animals taken to taken Total number of animals detect 5 % prevalence with 95%

) confidence ) of ) of (№ Pigs within goats cattle Sheep (№ (№ number number number Buffalo animals holdings village

Burgas

Sredec Debelt-1 0 0 57 1 984 219 0 59 1263 57 60 sheep-60

bovine-15;sheep- Sredec Dyulevo-2 0 0 58 287 1592 91 0 8 1978 58 54 29;goat-10

bovine-16;sheep- Sredec Zagorci- 3 0 0 31 319 532 36 0 0 887 57 60 43;goat-1

bovine-16;sheep- Sredec Zornitsa- 4 0 0 66 102 150 303 0 0 555 56 60 43;goat-1

Sredec Granitec- 5 0 0 8 16 164 37 0 0 217 51 60 bovine-5;sheep-55

Sinyo bovine-25;sheep- Sredec 0 0 6 260 294 158 0 0 712 57 60 Kamenene-6 33;goat-2

Valchanovo- bovine-12;sheep- Sredec 0 0 12 118 199 157 0 0 474 55 60 7 33;goats-15

bovine-12,sheep- Sozopol Dimchevo- 8 0 0 20 25 27 86 0 0 118 45 48 10,goats-26

bovine8,sheep152,goats- Sozopol Marinka- 9 0 0 97 12 610 198 0 0 820 57 194 34

68 bovine31, sheep-55 Sozopol Rosen-10 0 0 57 38 392 79 0 60 569 56 123 ,goats-19, pigs-18 Ravdinovo- bovine-41,sheep- Sozopol 0 0 32 102 792 184 4 26 1108 57 134 11 59,pigs-9,goats-25 bovine, Karnobat Iskra- 12 0 0 32 32 1054 17 0 0 1103 57 156 sheep, goats bovine, Karnobat Klikach- 13 0 0 84 75 928 10 0 0 1013 57 164 sheep, goats bovine-60 sheep-60 Aytos Pirne-14 0 0 138 432 1710 85 - 174 2401 58 208 goats-50 pigs-38 bovine-50 Aytos Sadievo- 15 0 0 68 71 890 45 - - 1006 57 115 sheep-50 goats-15 bovine-50 sheep-60 Aytos Chukarka- 16 0 0 146 95 1000 80 - 15 1190 57 146 goats-30 pigs-6 bovine-29, sheep-43, Kameno Vinarsko-17 0 0 31 60 98 22 0 6 186 50 100 goats-22, pigs-6

Balgarovo- bovine-45,sheep- Burgas 0 0 104 113 1087 219 0 0 1419 58 128 18 52,goats-31

Kableshkovo- bovine-2; sheep-60; Pomorie 0 0 22 2 1600 36 0 18 1656 58 90 19 goats-10; pigs-18

bovine-47; sheep-63; Nesebar Tankovo- 20 21 20 16 125 3000 24 0 0 3149 58 137 goats-7; pigs-20

69 Total 20 villages 21 20 1085 2285 17103 2086 4 366 21824 1116 2157

All samples are negative for antibody to SP of the FMDV (Mono ELISA O-type Prionics)

FMD case in wild boar First FMD outbreak wave Second FMD outbreak wave Settlements where the blood sampling in the susceptible animals were carried out Fig 27 – location of the settlements in the regions outside the 3 km protective and 10 km surveillance zones zones around the second outbreak wave and included in Annex II of Decision2011/44/ЕС. (Varna, Shumen, Sliven, Yambol and Haskovo)

70 Table 3 – Blood sampling in the susceptible animals in the settlements located in the regions outside the 3 km protective and 10 km surveillance zones zones around the second outbreak wave and included in Annex II of Decision2011/44/ЕС.

№ of Animals in the settlements blood Animal samples East Balkan pigs № of Total taken to № of holdings blood number detect 5 % blood Municipalities Settlements Total number of animals samples of prevalence samples taken per animals taken with 95% animals

) confidence ) of ) of (№ Pigs within goats cattle Sheep (№ (№ number number number Buffalo animals holdings village

Shumen region

bovine 14 Varbitsa Varbitsa- 21 0 0 244 152 508 105 0 0 765 57 57 sheep35 goats 10 bovine 16 Byala reka- Varbitsa 0 0 287 258 1009 30 0 0 1297 58 58 sheep 35 22 goats 12 bovine 10 Smyadovo Rish-23 97 53 197 61 1590 205 0 0 1856 58 58 sheep 25 goats 23 bovine 8 Veselinovo- Smyadovo 842 229 151 146 187 71 0 0 404 55 55 sheep 30 24 goats 17

Varna region

71 Bovine 45, sheep Dalgopol Partizani-25 0 0 262 100 600 200 0 27 1527 121 122 43 , goats-15, pigs 19 Sheep Asparuhovo- Dalgopol 0 0 123 0 140 55 0 0 195 50 50 44,goats 26 6 Bovine 26,sheep Dolni Chiflik Golitsa-27 150 50 217 30 600 100 0 0 880 134 135 52, goats 7,pigs 50

Dolni Chiflik Yunety- 28 0 0 0 0 0 0 0 0 0 0 0 0

Sliven region

54- bovine Kotel Kotel- 29 0 0 22 369 986 170 0 0 1525 112 112 57 sheep 1 goats 31- bovine Sliven Ichera- 30 0 0 9 40 326 217 0 60 643 127 127 54 Sheep 4 goats 38Pigs 58- Samuilovo- Sliven 0 0 46 1242 2800 0 0 0 4042 116 116 bovine 31 58-Sheep 57- bovine Sliven Kermen- 32 0 0 80 736 3720 0 0 28 4484 139 139 58 Sheep 24Pigs

Yambol region

72 bovine - 44 Vodenichene- Straldja 0 0 45 114 211 63 0 920 1308 58 154 Sheep-29 33 Goats-22 Pigs-59 bovine - 34 Straldja Tamarino- 34 0 0 51 34 1220 60 0 0 1365 58 99 Sheep-27 Goats-38 Kamen vrah- Sheep-18 Straldja 0 0 6 0 21 19 0 0 40 31 37 35 Goats-19 bovine - Mamarchevo- 18 Bolyarovo 0 0 40 18 53 31 0 0 597 56 102 36 Sheep-53 Goats-31 bovine - Golyam 1,Sheep- Elhovo 0 0 11 1 38 19 0 0 130 48 58 dervent- 37 38,Goats- 19 bovine - 56 Tundja Roza-38 0 0 81 291 600 180 0 30 1182 57 188 Sheep-56 Goats-50 Pigs-26

Haskovo region

goats-52 sheep-59 Topolovgrad Knyajevo- 39 0 0 0 22 401 132 0 0 555 133 133 bovine- 22 bovine- Svilengrad Varnik-40 0 0 4 56 67 18 0 1 142 48 80 38 sheep-42 bovine- Svilengrad Matochina-41 0 0 21 37 240 43 0 3 323 54 84 31 sheep-53

73 Total 20 villages 1089 332 1897 3707 15317 1718 0 1069 23260 1570 1964

All samples are negative for antibody to SP of the FMDV (Mono ELISA O-type Prionics)

74 Annex XI Clinical examinations and blood sampling of susceptible animals in the villages in the risk municipalities of Burgas and Yambol regions for proving absence of FMD virus circulation in domestic livestock in the areas north and west of the detected outbreaks. (28 - 29 .04.2011)

Fig 28-settlements located in 3 km protective and 10 km surveillance zones around the second outbreak wave.

Table – Blood sampling in the susceptible animals in the settlements located in 3 km protective and 10 km surveillance zones around the outbreaks ( second outbreak wave) Total № of № of № of Municipalities Settlements Animal Animals in the settlements number of blood blood blood holdings animals samples samples samples taken to taken taken per

75 ) № Pigs goats cattle Sheep Buffalo number ( number

Settlements in 3 km protective zones around the outbreaks IT- 4 to IT-11

cattle-45 Kirovo 4 150 7 17 0 0 174 50 65 sheep-7 goats-13

bovine-58 Golyamo 14 145 209 62 0 0 416 55 118 sheep-46 Bukovo goats-14 bovine- 113 Fakiya 64 180 469 288 38 0 975 57 467 sheep-176 goats-178 Sredets sheep-8 municipality, Trakiitsi 4 8 115 0 88 211 51 68 goats-45 Burgas region pigs-15 Granichar* 0 0 0 0 0 0 0 0 0 0

bovine-30 Gorno 10 84 370 52 0 0 506 56 147 sheep-116 Ybulkovo goats-1 Dolno 0 0 0 0 0 0 0 0 0 0 Yabalkovo*

bovine-11 Momina 66 57 2194 161 0 57 2412 58 141 sheep-128 Tsurkva goats-2

Malko Bliznak* 0 0 0 0 0 0 0 0 0 0

76 Turnovo municipality, Burgas region Evrenozovo 2 12 14 10 0 23 59 38 0 0

settlements in 10 km surveillance zones the outbreaks IT- 4 to IT-11 bovine- Bogdanovo 22 375 175 121 0 41 712 57 258 101 sheep-109 goats-48 bovine- Varovnik 2 216 0 0 0 0 216 51 86 86

Sredets bovine-53 Belevren 4 77 0 7 0 0 84 42 59 municipality, goats-6 Burgas region

Vulchanovo 0 0 0 0 0 0 0 0 0 0

bovine-26 Slivovo 8 31 161 60 0 0 252 53 128 sheep-77 goats-25

Zvezdets bovine-56 Malko 15 146 106 63 79 44 453 56 56 Turnovo municipality, Burgas region Mladezhko 9 0 0 40 0 0 40 31 31 goats-31

Golyamo 30 0 504 305 0 0 809 57 56 sheep28 krushevo goats28

bovine 28 Bolyarovo Gorska 18 109 60 80 0 0 249 53 72 sheep21 municipality, polyana Burgas region goats23 bovine -28 Strandzha 13 407 80 50 0 0 537 56 61 sheep-27 goats- 6

77 20 Total 285 1989 4357 1431 117 253 8105 821 1813 villages

All samples are negative for antibody to SP of the FMDV (Mono ELISA O-type Prionics)

78 Annex XII Clinical examinations and blood sampling of susceptible animals in the villages in the risk municipalities of Burgas and Yambol regions as well as in other villages in Burgas, Varna, Shumen, Sliven, Yambol and Haskovo regions for proving absence of FMD virus circulation in domestic livestock in the areas north and west of the detected outbreaks (18 - 19.05.2011)

Fig 29-settlements located in 3 km protective and 10 km surveillance zones around the second outbreak wave.

Table 1. Blood sampling in the susceptible animals in the settlements located in 3 km protective and 10 km surveillance zones around the outbreaks ( second outbreak wave)

79 Animal № of Animals in the settlements holdings blood samples № of taken to № of Total blood

) detect 5 % blood Municipalities Settlements number of samples № prevalence samples animals taken per with 95% taken

Pigs animals goats cattle Sheep

Buffalo confidence

number ( number within village

Settlements in 3 km protective zones around the outbreaks IT- 4 to IT-11

bovine-30 Kirovo 4 150 7 17 0 0 174 50 47 sheep-4 goats-13

bovine-62 Golyamo 14 145 209 62 0 0 416 55 121 sheep-44 Bukovo goats-15 bovine-83 Fakiya 64 180 469 288 38 0 975 57 213 sheep-71 goats-59 sheep-6 Sredets Trakiitsi 4 8 115 0 88 211 51 42 goats-24 municipality, pigs-12 Burgas region Granichar* 0 0 0 0 0 0 0 0 0 0

bovine-35 Gorno 10 84 370 52 0 0 506 56 155 sheep-115 Ybulkovo goats-5 Dolno 0 0 0 0 0 0 0 0 0 0 Yabalkovo*

bovine-24 Momina 66 57 2194 161 0 57 2412 58 203 sheep-165 Tsurkva goats-14

Malko Bliznak* 0 0 0 0 0 0 0 0 0 0 Turnovo

80 bovine- 4,goats- Evrenozovo 2 12 14 10 0 23 59 38 26 10.sheep- 12 settlements in 10 km surveillance zones the outbreaks IT- 4 to IT-11

bovine-60 sheep-64 Bogdanovo 22 375 175 121 0 41 712 57 165 goats-29 pigs-12

Varovnik 2 216 0 0 0 0 216 51 55 bovine-55

Sredets municipality, bovine-36 Belevren 4 77 0 7 0 0 84 42 42 Burgas region goats-6

Vulchanovo

bovine-19 Slivovo 8 31 161 60 0 0 252 53 92 sheep-50 goats-23

bovine-88, bufolloes- Zvezdets 15 146 106 63 79 44 453 56 297 40,goats- Malko 46, sheep- Turnovo 87,pigs-36 municipality, Burgas region

Mladezhko 9 0 0 40 0 0 40 31 31 goats-31

Bolyarovo Golyamo sheep28 municipality, 21 0 504 305 0 0 809 57 56 krushevo goats28 Burgas region

81 bovine -25 Gorska 18 109 60 80 0 0 249 53 68 sheep-20 polyana goats-23

bovine -36 Strandzha 13 418 80 50 0 0 548 56 82 sheep-27 goats-19

20 Total 276 2000 4357 1431 117 253 8116 821 1695 villages

82 FMD case in wild boar First FMD outbreak wave Second FMD outbreak wave Settlements where the blood sampling in the susceptible animals were carried out Fig 30 –– Blood sampling in the susceptible animals in the settlements located in the regions outside the 3 km protective and 10 km surveillance zones around the second outbreak wave included in Annexes II of Decision2011/44/ЕС (regions of Varna, Shumen, Sliven, Yambol and Haskovo)

Table 2 – Blood sampling in the susceptible animals in the settlements located in the regions around the 3 km protective and 10 km surveillance zones around the second outbreak wave and included inAnnex II of Decision2011/44/ЕС

№ of blood Animals in the settlements Animal samples East Balkan pigs holdings Total taken to № of № of blood number detect 5 % blood samples Municipalities Settlements of prevalence samples taken per Total number of animals animals with 95% taken animals confidence

) within ) of ) of pigs (№ goats cattle village Sheep (№ (№ number number number Buffalo animals holdings Burgas region Nesebar Priseltsi- 1 0 0 8 0 211 200 0 0 411 55 100 sheep-45, bovine-50;goats-55 Ruen Rojden- 2 0 0 0 120 419 0 0 0 539 56 100 sheep-50 Sungurlare Kamensko- 3 0 0 9 127 114 0 0 0 241 51 97 bovine;sheep Varna region EBP 30- Byala Dulino- 4 77 0 80 30 0 0 0 0 140 72 77 sheep 23, goats 24

83 47 Dolni chiflik Bulair- 5 0 49 119 334 20 0 0 49 473 101 101 bovine,sheep 48 ,goats 6

Shumen region bovine 17 Aleksandrovo- sheep 13 Smyadovo 0 0 39 89 30 30 0 221 350 54 54 6 goats 10 pigs 14 bovine 22 Varbitsa Kraigortsi- 7 0 0 67 50 286 7 0 0 343 54 54 sheep 25. goats 7 Sliven region 40-bovine Kotel Gradtes- 8 0 0 5 64 0 183 0 0 247 90 90 50 goats Yambol region bovine -55 Straldja Malenovo- 9 0 0 33 378 420 42 0 0 840 57 135 sheep-60 goats-20 bovine -40 Lyuilin- 10 0 0 40 77 110 32 0 0 219 51 84 sheep-33 goats-11 Valchi izvor- sheep-20 Bolyarovo 0 0 7 0 45 80 0 0 125 47 43 11 goats-23 Haskovo region sheep-60 Topolovgrad Radovets- 12 0 0 0 91 488 290 0 0 869 150 150 goats-50 bovine-40 bovine-3 Svilengrad Shtih -13 0 0 41 4 220 60- 0 11 295 54 54 sheep-36 goats-15 Chernodab- Svilengrad 0 0 6 26- 0 0 0 0 26 23 23 bovine-23 14

Total 14-villages 77 49 454 1390 2363 924 0 281 5118 915 1162 All samples are negative for antibody to SP of the FMDV (Mono ELISA O-type Prionics)

84 Annex XIII Clinical surveillance of susceptible livestock in the”Cordon Sanitaire” (16 - 25.08.2011)

Fig.31 Map with the location of the three sampling areas within “Cordon Sanitaire”: Sampling area A - Infected area (Tsarevo-south part (1) and Malko Tarnovo (2) and Sredets - south part (5’) municipalities) and Sampling area B - risk area (Tsarevo-north part (1’) Primorsko (3), Sozopol (4), Sredets -central part of (5’), and Sampling area C - risk area (parts of Bolyarovo (6), Elhovo (7), Topolovgrad (8), Svilengrad (9) municipalities)

Table – Blod sampling in the susceptible animals in the settlements located in the area”Cordon Sanitaire”.

85 № of Animals in the settlements blood samples № of taken to № of blood blood Village detect 5 % Municipalities Settlements samples samples Status prevalence taken taken per holdings with 95% animals pigs goats cattle Sheep confidence Buffalo Number of animal Number within

Total number of animals of number Total village

Burgas region

10-bovine, Tsarevo Ahtopol A 28 127 1 752 230 0 128 2237 58 58 20-sheep, 20-goats, 8-pigs 5-bovine, 24-sheep, Brodilovo A 56 9 392 672 0 43 1116 57 57 20-goats, 8-pigs

35-sheep, Balgari A 7 0 146 34 0 0 180 50 43 8-goats

14-bovine, Varvara A 15 33 308 186 0 0 527 56 56 26-sheep, 16-goats

Kosti* A 0 0 0 0 0 0 0 0 0 0

Rezovo* A 0 0 0 0 0 0 0 0 0 0

86 13-bovine, Velika Б 5 61 100 104 0 0 265 53 53 15-sheep, 25-goats

Izgrev A 3 0 8 0 0 12 20 19 6 6-pigs

25-sheep, Kondolovo A 3 0 144 98 0 0 242 52 52 27-goats

20-sheep, Lozenec Б 16 7 483 516 0 15 1021 57 57 37-goats

8-bovine, Sinemorets A 12 17 376 95 0 9 497 56 56 24-sheep, 24-goats

20-bovine, Fazanovo Б 7 162 762 14 0 46 984 57 57 37-sheep

10-bovine, Tsarevo A 38 74 3 232 319 0 0 3625 58 57 30-sheep, 17-goats 12-bovine, 3-бв, 11- Sozopol Varshilo B 26 260 218 192 10 697 1377 58 58 sheep, 7- goats, 25- pigs

10-goats, Gabar B 6 1 660 110 0 0 771 57 57 47-sheep

5-bovine, 24-sheep, Zidarovo B 182 243 884 714 0 263 2104 58 58 18-goats, 11-pigs

87 46-sheep, Izvor B 185 4 3 605 559 0 241 4409 59 59 10-goats, 3-pigs 2-bovine, Indzhe Voyvoda B 43 16 424 481 0 88 1009 57 54 23-sheep, 29-goats 12-bovine, 30-sheep, Krushevets B 81 309 1 518 460 0 354 2641 58 58 10-goats, 6-pigs

36-bovine, Sredets Belevren A 5 76 0 18 0 0 94 43 43 7-goats

20-bovine, 20-sheep, Bogdanovo B 22 171 347 221 0 41 780 57 57 9-goats, 8- pigs

52-bovine, Varovnik B 3 219 0 0 0 11 230 53 53 1-pigs

15-bovine, Valchanovo B 39 113 334 272 0 0 719 57 57 25-sheep, 17-goats 16-bovine, Golyamo A 15 131 137 96 0 3 367 54 54 17-sheep, Bukovo 21-goats 16-bovine, Gorno A 10 77 612 108 0 0 797 57 57 37-sheep, Yabalkovo 7-goats 7-bovine, Granitez B 13 16 275 72 0 0 363 55 56 37-sheep, 12-goats

88 Granichar* A 0 0 0 0 0 0 0 0 0 0

Dolno A 6 0 0 0 0 0 0 0 0 0 Yabalkovo*

27-bovine, 13-goats, Drachevo B 48 385 251 263 0 143 1042 57 57 11-sheep, 6-pigs 40-bovine, Kirovo A 4 147 11 28 0 186 51 51 5-sheep, 6-goats 12-bovine, Momina Carkva A 66 57 2 925 283 0 57 3322 58 58 36-sheep, 10-goats

23-bovine, 7-goats, Panchevo B 12 159 882 117 0 12 1170 57 57 23-sheep, 4-pigs

20-bovine, Rosenovo B 14 137 169 142 0 0 448 55 55 19-goats, 16-sheep 14-bovine, Sinyo Kamenovo B 9 260 507 160 0 0 927 57 57 19-goats, 24-sheep 10-bovine, Slivovo B 12 36 292 159 0 0 487 56 56 20-sheep, 26-goats 5-sheep, Trakiici A 4 0 14 113 0 89 216 51 51 39-goats, 7-pigs

89 24-bovine, Fakia A 64 198 722 550 38 0 1508 58 58 10-sheep, 24-goats

Novo 18-bovine, Б 61 148 2 163 141 0 59 2511 58 58 Panicharovo 40-sheep

10-bovine, Primorsko Pismenovo Б 20 14 104 23 0 9 150 49 50 33-sheep, 7-goats

18-bovine, Yasna Polyana Б 61 58 1 315 123 20 43 1559 58 58 40-sheep

Malko Bliznak* А 0 0 0 0 0 0 0 0 0 0 Tarnovo

Brachlyan А 3 6 0 0 0 0 6 6 6 6-bovine

6-bovine, 24-sheep, Byala voda А 4 6 46 82 0 44 178 51 51 15-goats, 6-pigs 15-bovine, Vizica А 6 44 148 4 0 0 196 51 51 1-goats, 35-sheep

Gramatikovo* А 0 0 0 0 0 0 0 0 0 0

5-bovine, 13-sheep, Evronozowo А 2 11 14 22 0 28 75 34 34 9-goats, 7- pigs

90 11-sheep, Zabernovo А 9 0 11 175 0 73 259 54 54 39-goats, 4-pigs 17-bovine, 10-sheep, Zvezdec А 15 226 89 102 79 32 528 56 56 24-goats, 5-pigs 25-sheep, Kalovo А 5 0 57 59 0 44 160 49 49 20-goats, 4-pigs 25-sheep, Malko Tarnovo А 75 132 1 654 479 0 165 2430 58 58 15-goats, 8-pigs

Mladezko А 9 0 0 80 0 0 80 31 31 31-goats

20-goats, Slivarovo А 4 0 71 65 0 7 143 45 45 25-sheep

35-goats, Stoilovo А 23 0 106 149 0 0 255 55 55 20-sheep

Yambol Region

bovine-17 Bolyarovo Bolyarovo C 69 220 843 213 0 20 1296 57 57 goats-10 sheep-30 Small Voden C 20 160 1 220 173 0 10 1563 58 58 ruminants- 58

sheep-24 Valchi izvor C 10 0 45 80 0 0 125 47 47 goats-23

91 Glyamo C 20 0 504 350 0 60 914 57 57 sheep-57 Krushevo

bovine-35 Gorska polyana C 10 38 60 80 0 0 178 50 50 sheep-11 goats-4

sheep-27 Denitsa C 16 0 55 50 0 3 108 45 45 goats-18

bovine-35 Dabovo C 20 116 107 95 0 0 318 54 54 goats-10 sheep-9

sheep-55 Zlatinitsa C 30 96 296 15 0 2 409 55 55 bovine -49

Iglika C 5 122 40 0 0 0 162 49 50 bovine -49

bovine -5 small Kraynovo C 10 62 476 35 0 0 573 56 56 ruminants- 51 small Malko Sharkovo C 9 457 100 80 0 5 642 56 56 ruminants- 56

Mamarchevo C 25 60 425 95 0 10 590 56 56 goats-56

bovine-38 Oman C 15 122 100 20 0 3 245 53 53 sheep-8 goats-7

Popovo C 130 459 270 166 0 5 900 57 57 bovine-57

92 44/the total number v Ruzhitsa C 12 71 0 120 0 0 191 51 animals goats-44 within the village/

Sitovo C 14 422 37 71 0 0 530 56 56 bovine -56

bovine -10 Stefan C 80 95 800 357 0 12 1264 57 57 sheep-32 Karadzhovo goats-15

bovine -15 Strandzha C 9 350 85 62 0 0 497 56 55 sheep-40

Sharkovo C 15 67 1 220 123 1 10 1421 58 58 sheep-58

Bovine - Elhovo Valcha polyana C 31 78 297 0 0 0 375 55 55 11 Sheep -44 Small C 13 1 61 68 0 0 130 48 48 ruminants- Golyam dervent 48 Bovine-4 Granitovo C 43 70 1 556 93 0 15 1734 58 58 Goats-4 Sheep-50 bovine-21 Lakovo C 25 317 411 106 0 0 834 57 57 Goats-6 Sheep-30

Bovine-7 Lesovo C 130 192 383 941 0 5 1521 58 58 Goats-51

93 Bovine-25 Malko Kirilovo C 8 93 87 11 0 0 191 51 51 sheep-26

Bovine - 22 Malomirovo C 36 336 162 267 0 14 779 57 57 Sheep-33 Pigs-2 Bovine - 19 Melnitsa C 52 360 310 253 0 28 951 57 57 Goats-17 Sheep-19 Pigs-2 Bovine - 13 Razdel C 63 211 185 457 0 2 855 57 57 Sheep-42 Pigs-2 Bovine - 32 Slaveikovo C 10 226 0 26 0 4 256 53 53 Goats-20 Pigs-1 Bovine - Chernozem C 19 235 338 195 5 22 795 57 57 12 Sheep-45

Haskovo region

Sheep-41 Topolovgrad Knyazhevo C 41 19 401 132 0 0 552 56 56 Bovine-3 Goats-12 Sheep 12 Planinovo C 15 31 60 120 0 0 211 51 51 Bovine-10 Goats-29

Prisadetz C 2 53 0 5 0 0 58 35 35 Bovine-35

94 Sheep-27 radovets C 152 91 488 290 0 0 869 57 57 Bovine-6 Goats-24 Sheep-36 Srem C 41 65 708 219 0 0 992 57 57 Bovine-5 Goats-16 Sheep-28 Ustrem C 152 492 949 636 0 0 2077 58 58 Bovine-16 Goats-14

Filipovo C 3 83 5 0 0 0 88 42 42 Bovine-42

Sheep-22; Bovine- Svilengrad Varnik C 4 56 67 18 18 0 142 48 48 17, Goats-9

Sheep-20 Generalovo C 13 12 16 2 0 0 30 26 26 Bovine-6

sheep-15 Dervishka C 14 5 67 72 0 9 153 48 48 Bovine-3 mogila Goats-30 Sheep-10 Dimitrovche C 82 5 46 233 0 48 332 54 54 Bovine-3 Goats-41 Sheep-29 Kapitan C 107 46 245 62 0 40 393 55 55 Bovine-10 Andreevo Goats-16 Sheep-30 Levka C 135 399 343 201 0 32 945 57 57 Bovine-17 Goats-10 Sheep-36 Matochina C 21 37 240 43 0 3 323 54 54 Bovine-15 Goats-3

95 Sheep-31 Mihalich C 42 2 121 94 0 10 227 51 51 Goats-20

Bovine-6 Mustrak C 91 16 0 279 0 5 300 54 54 Goats-48

Sheep-30 Pashov C 9 81 74 0 2 157 49 49 Bovine-19

Sheep-37 Ravna Gora C 6 3 67 16 0 0 86 42 42 Goats-5

Sheep-23 Raikova mogila C 110 0 189 158 0 15 362 54 54 goats-31

Sheep-20 Sladun C 52 0 47 157 0 11 215 51 51 Goats-31

Sheep-35 Studena C 252 137 1 022 743 23 99 2024 58 58 Bovine-8 Goats-15

Bovine-10 Shernodab C 6 11 40 0 0 3 54 35 35 Sheep-25

Sheep-30 Shtit C 41 4 220 60 0 11 295 53 53 Bovine-2 Goats-21

Total 106 villages 3696 10834 44156 17032 194 3274 75443 5232 5203

Note – no susceptible animals in Kost, Rezovo, Gramatikovo, Granichar, Bliznak, Dolno Yabalkovo because detection of FMD outbreak in these villages ( outbreaks IP-a, IP-2, IP-3, IP-5, IP-10 and IP 11)

All samples are negative for antibody to SP of the FMDV (Mono ELISA O-type Prionics)

96 Annex XIV Surveillance in wildlife; January – August 2011

Fig 32 -the location of the traps for wild boar in Tsarevo and Malko Tarnovo municipalities

97 Fig 33 -surveillance in wildlife – January –August 2011

Table. Serological surveillance in wildlife in the period January –August 2011) Number of sampled Ab positive FMDV positive wild boar (Sus scrofa) 89 2 0 roe deer (Capreolus capreolus) 36 3 0 red deer (Cervus elaphus) 2 0 0 Total 127 5 0

98 Annex XV Time of FMD events in Bulgaria 2010 - 2011

FMD Timeline - Kosti and Resovo, Bulgaria.

Green boxes are Observed Events (Surveillance findings) 7th Jan: Kosti Healing Lesion seen in domestic pig (=IP1). 5 +ve pigs and 1 +ve cow in 27th December: infection in IP1 must group (FMD-NSP antibody). Entry of infection to IP1 (Kosti) in period 13- have occurred on or before this date to Blood sampling Kosti Hereford herd (IP3): Kosti - Surveillance finds FMD signs 4 days age 27th based on lesion (assumed 10 days) seen give rise to clinical signs/NSP+ves at NSP-ve. Incubating? in Hereford herd (=IP3) in domestic pig on 7th Jan sampling on 7th Jan.

ENTRY TO IP3 1ST LESIONS-IP3 EU team visit Resovo. Oldest SPREAD WITHIN KOSTI VILLAGE (=IP 2).LEADS TO 27 +VE AT SAMPLING ON 7TH lesion observed 7-10 days IP1 infectious - spread age. 1st lesions estimated to occur Resovo 8- 10th

26-Nov 28 N 30thN 2 De 4 De 6 De 8 De 10 De 12 De 14 De 16De 18De 20De 22De 24De 26De 28De 30De 2Ja 4Ja 6Ja 8Ja 10Ja 12Ja 14Ja 18Ja 20Ja 22Ja

4th : Turkish buffalo reported in Resovo Earliest infection date (14 day incub.) 1st day Wild boar (B)observed 1st Day est CS 3 Wildboar shot, 1 for Wild boar B estimated lame (Slivarovo Hunting in wildboar lame.FMD confirmed lesions for area). case (A). 4th Jan Wild Boar B

MINIMUM PERIOD OF INFECTIOUS WILD BOAR (SUSPECTED AND CONFIRMED CASES ONLY)

99 MINISTRY OF AGRICULTURE AND FOOD BULGARIAN FOOD SAFETY AGENCY * Sofia, 1606, “Pencho Slaveikov” blvd. 15A ( +359 (0) 2 915 98 20, +359 (0) 2 954 95 93, www.babh.government.bg

SAFETY EVERY DAY

ADDENDUM

to the dossier submitted to OIE on 5th June 2012 on the Foot-and-Mouth Disease epidemiological situation in Bulgaria and the application by the Bulgarian Food Safety Agency for recovery of the former status of the whole territory of Bulgaria as a

“FMD FREE COUNTRY WHERE VACCINATION IS NOT PRACTICED”

20/07/2012

1. Introduction On 5th January 2011, Bulgaria notified a case of FMD in a wild boar shot 2 km from the Turkish border in the Region of Burgas. This was the first detection of FMD virus in Bulgaria since 1996. Until 7th April, the date of the last outbreak, 11 type O outbreaks were confirmed in livestock in 10-12 km border area which stretches into Turkish Thrace.

Despite of the fact that Bulgaria was FMD free country where vaccination is not practiced for 16 years and all outbreaks in 2011 were located at a distance of no more than 12 km from the Turkish Border the status of the whole territory of the country (110 994 km2) was suspended and Bulgaria is not currently included among those countries with officially recognized FMD-free status according to OIE Resolution No 14 (adopted during the 80th General Session of the OIE International Committee, May 2012).

On 5th June 2012 Bulgaria submitted to OIE dossier, applying for the recovery of 'FMD free country where vaccination is not practiced' status.

A preliminary screening of the dossier performed at OIE Headquarters revealed that the data provided in the application is from the restricted zone only and did not cover the whole country for which the recovery of status was requested. (Letter by Dr Bernard Vallat of 21st June 2012, Ref.: KHO/KM/SB 30.695).

However the FMD incursion in 2011 was only limited to a small area in the South-East Bulgaria which was clearly proved by the surveillance program implemented successfully by the Bulgarian Food Safety Agency. The dossier provided by Bulgaria clearly shows that the spread of FMD was stopped at the border area. All outbreaks were located in the 12 km zone along the border with Turkey. The surveillance carried out in the protection and surveillance zones as well as in the so called restricted area (Cordon Sanitaire) and in certain villages to the north of this area did not detect any evidence of FMDV circulation for more than 14 months after the last outbreak. The surveillance in domestic livestock has clearly shown disease absence. The relatively low sero-prevalence and clustered spatial distribution of sero-positive wild boar and roe deer suggest that the FMD infection failed to develop into a large scale epidemic wave in wildlife and faded out within less than a year. The FMD incursion was contained by establishing the “Cordon sanitaire” supported by the fact that neither clinical signs nor sero-conversion has been observed in herds of East-Balkan pigs kept on pastures all year just north of this or in other parts of Bulgaria. No clinical signs suggesting FMD have been observed during the regular veterinary activities and clinical surveillance in animal holdings and slaughterhouses on the whole territory of Bulgaria during April 2011 – July 2012.

As an OIE Member country Bulgaria has always implemented all its obligations to the organization and the other Member country. Annual reports indicating the FMD status have been provided regularly. Immediate notifications and follow up reports in case of outbreaks of infectious diseases as well as the regular six months and annual reports for the animal health status of the country have always been regularly submitted. Bulgaria was fare enough to notify to OIE/WAHIS and the world even the only detected FMDV positive wild boar despite of the very high price that the country had to pay. Since April 2011 no FMD outbreaks have been notified through WAHIS because no such outbreaks have been observed on the whole territory of Bulgaria.

Bulgaria considers that fulfills the requirements of articles 8.5.2, 8.5.9 point 1(a), 8.5.45, 8.5.47 and 8.5.49 of the OIE Terrestrial Animal Health Code.

In this addendum Bulgaria provides additional information related not only to the restricted zone but to the whole territory of the country for proving its FMD freedom. Short information is provided on the history of the FMD outbreaks in the country in the 90’s; summary of the FMD situation in 2011 and 2012; some of the conclusions of the EFSA’s Scientific Opinion on Foot-and-mouth disease in Thrace; the control system in place for animal health (particularly FMD) and the clinical surveillance of the animal health status of FMD susceptible livestock on the whole territory of Bulgaria during 2011 - 2012.

2. History of FMD outbreaks in Bulgaria In the 1990’s Bulgaria experienced three FMD type O1 outbreaks, all in the Thrace region (Figure 1).

The first outbreak (O/BUL/1/91) was reported in July 1991. During that period Bulgaria had already stopped prophylactic vaccination against FMD in the established 30 km buffer zone bordering Turkey. The outbreak was restricted to Stefan Karadjovo village (1), Yambol region. Stamping out and ring vaccination was successfully applied as the control policy.

In May 1993, a second FMD outbreak was notified. The disease was detected in a dairy cattle holding near the town of Simeonovgrad (2), Haskovo region, located very close to

2 an international transit road. For the second time, stamping out with ring vaccination was successfully applied. In total, 304 infected and contact cattle were killed and buried.

The third outbreak was notified in October 1996 in the village of Malko Sharkovo (3), Yambol region, and stamping out was the control policy used to eradicate the outbreak. No vaccination policy was applied. The hypothesis for the origin of the disease was that the virus was introduced with illegally moved susceptible animal(s) from Turkey.

1= Stefan Karadjovo 1991; 2= Simeonovgrad 1993; 3= Malko Sharkovo 1996

Figure 1: Location of FMD outbreaks in Bulgaria in the 90’s

3. Summary of the FMD situation in Bulgaria in 2011 and 2012

On 5 January 2011 the Bulgarian competent authorities reported a confirmed case of FMD in a wild boar (with lesions indicative of FMD) which had been shot in the Burgas region about two kilometers north of the Turkish border on 30 December 2011. Isolation and typing of the virus were made by the EU Reference Laboratory (EU-RL).

Measures in accordance with Council Directive 2003/85/EC of 29 September 2003 on Community measures for the control of foot-and-mouth disease repealing Directive 85/511/EEC and Decisions 89/531/EEC and 91/665/EEC and amending Directive 92/46/EEC were implemented from 5 January 2011. Clinical and serological surveillance was initiated and 11 outbreaks in domestic animals were detected in south-east Bulgaria between 9 January and 7 April. These were the first outbreaks of FMD in Bulgaria since 1996.

All the FMD viruses sampled in Bulgarian Thrace were the serotype O PanAsiaII-Ant10 lineage.

3 Certain protection measures against FMD in Bulgaria were introduced on 6 January 2011 through Commission Decision 2011/8/EU of 6 January 2011 concerning certain interim protection measures against foot-and-mouth disease in Bulgaria, which was repealed by Commission Decision 2011/44/EU of 19 January 2011. Protection measures applied until 30 September 2011.

Stamping-out and strict surveillance (Cordon sanitaire), but no vaccination was applied to eradicate FMD in domestic animals;

Protection zones and surveillance zones in line with the EU legislation had been implemented around each of the 11 FMD outbreaks. The protection and surveillance zones around the second wave of outbreaks (outbreaks 4-11) detected 19 March – 7 April 2011 were merged forming a common inner protection zone and outer surveillance zone until all measures in each zone were finalized for all eight outbreaks;

On 18-19 April 2011 clinical examinations were carried out and 2030 samples were collected from susceptible animal species in the villages inside the protection or surveillance zones where there were still animals. All samples were negative for antibodies to FMD serotype O.

Sampling and clinical examination were carried out in the villages inside the protection and surveillance zones on 28-29 April and on 18-19 May 2011. All samples were negative for antibodies to FMD serotype O.

On 18-19 April and on 18-19 May 2011 clinical examinations and sampling were carried out in randomly selected villages (40 and 14, respectively) around the surveillance zone, i.e. more than 10 km from an FMD outbreak. Sample numbers for each village were in excess of the minimum numbers calculated for a 5% detection rate with 95% confidence. All samples were negative for antibodies to FMD serotype O;

On 4 July Order 11-713 lifted the protection and surveillance zones around outbreaks 4- 11, implemented the amended restricted zones in Annexes I and II to Commission Implementing Decision 2011/388/EU of 29 June 2011 amending Decision 2011/44/EU concerning certain protection measures against foot-and-mouth disease in Bulgaria (valid until 30 September 2011), and reiterated the applicable restrictions.

Surveillance in susceptible wildlife, particularly wild boar was initiated in January 2011. A plan for the eradication of FMD in wildlife, taking into account Part B of Annex XVIII of Council Directive 2003/85/EC, was submitted to the Commission on 4 April 2011 and approved by Commission Implementing Decision 2011/493/EU of 5 August 2011 approving the plan for the eradication of foot-and-mouth disease in wild animals in Bulgaria.

The main components of the Eradication Plan in the Cordon Sanitaire (see the dossier submitted on 5th June 2012) were: - movement restrictions on animals and products;

4 - active surveillance (shooting/trapping, sampling and laboratory analysis) of susceptible wild animals; - documented clinical examination of domestic animal herds in all 106 villages in the Cordon Sanitaire at least every 21 days; - at least every 90 days, blood sampling from susceptible animals for serological examination, aimed at detecting a 5% sero-prevalence with 95% confidence in each epidemiological unit (=village).

Under the Eradication Plan sampling of domestic animals in 106 villages in the Cordon Sanitaire was carried out for serological investigation on 16-25 August 2011 (5203 samples), 21-25 November 2011 (5295 samples) and 20-25 February 2012 (5334 samples).

A total of 812 wild boar, 68 roe deer, 7 red deer and 2 mouflons were tested for the presence of FMD virus genome and FMD antibodies between February 2011 and January 2012 in the same region. No FMD virus genome could be detected. However, sero- positive animals were found amongst wild boar and roe deer. The sero-prevalence in the wild boar was 6.9 % with a statistically significant difference between adults (9.5%) and juveniles born in 2011, (1.6 %). Three (4.4%) roe deer were found to be sero-positive. Most of the sero-positive animals were spatially clustered around the FMD outbreaks in livestock, limited within a radius of 20 km. The relatively low sero-prevalence and clustered spatial distribution of sero-positive wild boar and roe deer suggest that the FMD infection in wildlife was mainly a short timing disease event, which failed to develop into a large scale epidemic wave in wildlife and had fade out.

East-Balkan pigs were blood sampled between February 2011 and May 2012 in the regions of Burgas (553 samples), Shumen (558 samples) and Varna (80 samples). All samples were negative for antibodies to FMD virus serotype O.

Although the Eradication Plan ended 3 April 2012 all restrictions in the Cordon Sanitaire imposed were still in force till the end of June 2012; The intensive controls on movements of animals and products, which were implemented during 2011, were facilitated by a well functioning cooperation between the competent authorities involved.

After the Eradication Plan ended 3 April 2012, surveillance had continued in 37 (located in 10 km zone along the border with Turkey) of the 106 villages under a national surveillance plan. Sampling had been carried out in these 37 villages during 28-31 May 2012 when 1769 blood samples were collected from susceptible livestock. Sample numbers for each village were in excess of the minimum numbers calculated for a 5% detection rate with 95% confidence. All samples were negative for antibodies to FMD serotype O;

5 BULGARIA

Sredets

Malko Tarnovo Boliarovo Tsarevo Topolovgrad Elhovo

Svilengrad TURKEY

GREECE Village under surveillance Figure 2: Villages (n = 37) along the border where clinical and serological surveillance continues during April – December 2012.

Intensive monitoring of domestic animals during 14 months after the last outbreak has shown that the stamping out of the 2011 outbreaks was successful. The results from wildlife sampled until January 2012 indicate that the spread of FMD virus serotype O, which caused the 2011 outbreak, to wild animals had taken place only in the vicinity of the outbreaks in domestic animals and that the spread of the virus to juvenile wild boar (born in 2011) had been very limited and died out before mid summer of 2011.

4. Scientific Opinion on foot-and-mouth disease in Thrace. EFSA Journal 2012;10(4): 2635. 91 pp. doi:10.2903/j.efsa.2012.2635;

Following a request from the European Commission and the Risk Assessment Centre within the structure of the BFSA, the Panel on Animal health and Welfare at the European Food Safety Authority (EFSA) was asked to deliver a scientific opinion on FMD in Thrace.

A Scientific Opinion by EFSA on Foot-and-mouth disease in this geographical region (Thrace) was published on the EFSA website on 4 April 2012 together with an External Scientific Report on the epidemiology of FMD virus in wildlife in Thrace (http://www.efsa.europa.eu/en/publications/efsajournal.htm).

EFSA concluded that: - Most likely the disease died out in mid-summer 2011, and the areas subject to intensive surveillance both in Bulgaria and Turkey are now free from FMD in wildlife (and domestic animals). This is based on the observations with

6 epidemiological considerations (hot summer in 2011, which was detrimental for environmental survival of FMDV, a relatively low density of wild boar of ~2-3 heads km, absence of clinical signs or virus detections in a considerably large proportion of animals inspected and tested in Bulgaria in October- December 2011 exactly in the former area of the infection, as well as further away in Turkey). - The epidemiological model indicates that the presence of deer in the populated area does not alter the spatio-temporal dynamics of the infection in the model and that deer alone are not able to facilitate spread of the infection through the whole landscape. - The epidemiological model indicates that the strong temperature dependence of FMDV survival in the environment explains the seasonal increased chance of virus fade-out. - The epidemiological model indicates that continued maintenance (e.g. with moderately virulent CSFV in wild boar) cannot be expected from a wild boar + deer host system alone for FMDV. There is need for cross-transmission between wildlife sub-populations due to human movement or cross- transmission to the domestic sector for virus circulation to be maintained.

5. Control system in place for animal health (particularly FMD) in Bulgaria

Bulgaria is divided into 28 regions. The regions are administrative units executing the regional policy of central government bodies. The regional governors are appointed by the Council of Ministers. Local government is organised through 264 municipalities. At regional level BFSA has 28 Regional Food Safety Directorates (RFSD) responsible for the implementation of the official controls (RFSA in each administrative unit of the country). All FMD outbreaks in 2011 were detected only in the region of Burgas (02).

Competent authorities

7 The central competent authority for animal health is the BFSA. The BFSA is subordinated to the Ministry of Agriculture and Food (MAF). The Animal Health and Welfare Directorate (AHWD) of the BFSA takes part in the harmonization and drafting of legal implementing documents relevant to its competencies. It also arranges for veterinary preventive measures against infectious and parasitic diseases including FMD and prepares the annual prophylactic, monitoring and eradication programmes. It is also responsible for the preparation of contingency plans, guidelines and instructions for the RFSD on the implementing of animal health measures. The total number of staff involved in the animal health sector in the official services in Bulgaria is: BFSA, 28 RFSD, 160 veterinary auxiliaries, 265 OMV (official municipal veterinarians).

Furthermore, 1,025 authorised private veterinarians (APV) perform official tasks under the animal health programmes.

Holding registration, animal identification and movement controls

8 Animal holdings keeping cattle, pigs, sheep and goats are registered on the Central Database (CDB) named VetIS and a unique holding number is allocated to each holding. The CDB is an Internet based system designed for multi-species functionality. The AHWD is responsible for the management of the information in the CDB. In the CDB data is entered by 1,400 users, including private veterinarians, veterinarians performing official controls on holdings, slaughterhouses, collection centers and others. The national system of identification and registration of animals requires identification of all species by an individual number. The APV are responsible for tagging and registering the animals and providing monthly data to the OMVs who enter it into the CDB. A new module of the CDB was developed and entered into force.

The system for animal movement control is based on animal health movement certificates issued by the APV responsible for the farm. Passports are issued for large ruminants and equines. All holdings are required to keep holding registers and each change to the herd is entered in a record book.

Animal health controls Control measures (sampling, testing, and vaccination) prepared by the AHWD and approved by the MAF, are implemented by the RFSD, OMV and APV. There is an animal health department in each of the 28 RFSD. At local/district level, there are 265 municipalities each supported by an OMV who supervises the performance of the APV. A total of 1,025 APV carry out prophylactic and monitoring of the animal health programmes.

The APV report the results of their work on a monthly basis to the relevant OMV who summarises the input from all APVs and report to the RFSD. The RFSD reports to the AHWD on the implementation of the animal health programmes in their region on a monthly basis.

Specific eradication and monitoring programmes are approved for different animal diseases including FMD.

(The Control and coordination Directorate-CCD performs audits at all administrative levels (AHWD, RFSD, OMV and APV).

Control in slaughterhouses The total number of RFSD inspectors carrying out the veterinary public health controls (including inspection in slaughterhouses) is 617, of whom 495 are official veterinarians.

Contingency plans Updated of contingency plans for emergency diseases in Republic of Bulgaria are available on: http://babh.government.bg/bg/actualno-department-health&article_id=108.html

For practical purposes the BFSA has based its overall contingency plan on three major types: · A Strategy and Resource plan,

9 · Operational manuals – FMD + others · Instructions for practical matters during outbreak management.

The contingency plan for emergency diseases in Bulgaria (the strategy and resource plan, the operation manuals of the animal diseases and the practical instructions) is prepared in accordance with the requirements of national and European legislation, the OIE standards and characteristics of different diseases.

National reference laboratory (NRL) for FMD The National Diagnostic and Research Veterinary Institute (NDRVI) is a specialized unit within the the BFSA on diagnostics and research activities in the field of animal health. It includes the national reference laboratory (NRL) on FMD which operates under the coordination of the Laboratory Activities Directorate of the BFSA.

The NDRVI is accredited to the standards of International Organisation for Standardisation (ISO) 17025:2005 by the Bulgarian Accreditation Service. The separate location of the FMD NRL is specified in the current accreditation certificate, which is valid until 30 June 2013.

The NRL for FMD has a laboratory contingency plan for FMD comprising those methods which would be available for detection of FMD antibodies or antigen and comply with the requirements of the OIE diagnostic manual.

During the FMD epidemics the following tests were used: - serological tests used for surveillance are FMD ELISA Type O and/or FMD NSP ELISA and LPB ELISA serotype O - Virological tests used for FMD detection and surveillance are RT PCR and/or Ag ELISA

Staff responsible for FMD testing is well trained for the range of methods applicable and is well aware of the advantages and limitations of each method;

Samples are under the official control during the transportation to the FMD laboratory. Maximum sample turnaround times had been satisfactorily short (1-3 days) also during the peak of the 2011 outbreaks;

Whilst in the beginning of the 2011 FMD outbreaks serological samples were analysed using NSP-ELISA and LPBE (albeit using only reagents for serotype O), towards the end of the outbreaks and for the subsequent serological surveys under the Eradication Plan the vast majority of serum samples were screened only with the type O ELISA. Screening positive samples were re-tested with the same method or/and tested with NSP-ELISA combined with appropriate clinical and epidemiological investigations of the reactor animal at hand and appropriate clinical, epidemiological and serological, investigations of the of susceptible animals of the same epidemiological unit and of susceptible animal that have been in contact or otherwise epidemiologically associated with the reactor animal. If

10 the follow-up investigations provide no evidence for FMDV infection the reactor animal is only then classified as FMD negative.

6. Clinical surveillance of the animal health status of FMD susceptible animals on the whole territory of Bulgaria during 2011 -2012

The clinical and serological surveillance for FMD carried out in the area bordering Turkey as well as in certain villages to the north and west of this area is described in the dossier submitted to OIE on 5th July 2012

In addition clinical examinations of the FMD susceptible livestock have been carried out in 2011 and 2012 on the whole territory of Bulgaria in the framework of different animal health programs without detecting any evidence for FMD: a) Clinical examinations of the health status of bovines during the implementation of the control programs for bovine tuberculosis, bovine brucellosis & enzootic bovine leucosis in 2011 and 2012 (till end of June)

Out of the FMD outbreaks in Jan – Apr 2011 in Burgas region, no evidence of FMD have been detected in bovines during clinical examinations of their health status in 2011 and 2012 (as by end of June) on the whole territory of Bulgaria in the framework of the control programs for bovine tuberculosis, bovine brucellosis & enzootic bovine leucosis (EBL) (tables 1 and 2)

Table 1: Clinical examinations of bovines in 2011 without detecting any evidence for FMD

11 16 Plovdiv 6 321 63 498 6 321 57 387 6 321 36 578 6 321 18 268 17 Razgrad 2 820 18 435 2 820 19 433 2 820 13 766 2 820 3 538 18 Russe 1 528 14 876 1 528 12 111 1 528 9 088 1 528 3 325 19 Silistra 3 072 12 508 3 072 11 879 3 072 5 224 142 1 884 20 Sliven 381 27 616 381 24 335 381 16 792 173 8 969 21 Smolyan 8 165 15 222 8 165 12 585 8 165 8 572 204 694 22 Sofia town 376 4 919 376 4 662 376 2 849 376 1 823 23 Sofia-district 5 600 13 533 5 600 13 533 5 600 9 918 55 2 812 24 Stara Zagora 2 950 29 272 2 950 28 553 2 950 20 638 195 4 855 25 Targovishte 6 366 25 135 6 366 24 433 6 366 15 677 85 3 870 26 Haskovo 7 230 35 924 7 230 33 482 7 230 20 702 125 2 747 27 Shumen 4 544 24 225 4 544 24 225 4 544 16 108 176 6 428 28 Yambol 1 263 23 130 1 263 16 985 1 263 10 091 130 3 412 Total 103 383 586 434 103 382 537 426 103 383 351 506 15 894 108 672

Table 2: Clinical examinations of bovines in 2012 (by end of June) without detecting any evidence for FMD

Number of clinical Number of clinical Number of clinical examinations in the examinations in the examinations in the Total number of framework of control framework of the framework of the Region existing bovines program for bovine control program for control program for tuberculosis bovine brucellosis EBL herds animals herds animals herds animals herds animals 1 Blagoevgrad 6 540 29 133 4 905 21 850 4 905 20 393 39 928 2 Burgas 4 854 27 087 3 641 20 315 3 641 18 961 105 3 850 3 Varna 1 162 10 989 872 8 242 872 7 692 172 1 725 4 Veliko Tarnovo 2 440 21 281 1 830 15 961 1 830 14 897 2 440 3 750 5 Vidin 782 7 088 587 5 316 587 4 962 50 2 034 6 Vratsa 5 218 17 672 3 914 13 254 3 914 12 370 65 1 183 7 Gabrovo 956 7 700 717 5 775 717 5 390 41 1 264 8 Dobrich 1 460 22 903 1 095 17 177 1 095 16 032 171 5 492 9 Kardjali 13 849 48 915 10 387 36 686 10 387 34 241 38 1 162 10 Kjustendil 1 539 6 296 1 154 4 722 1 154 4 407 18 234 11 Lovech 1 425 10 582 1 069 7 937 1 069 7 407 39 1 934 12 Montana 1 558 15 691 1 169 11 768 1 169 10 984 92 2 454 13 Pazardjik 6 413 19 602 4 810 14 702 4 810 13 721 172 3 395 14 Pernik 1 399 5 876 1 049 4 407 1 049 4 113 11 761 15 Pleven 4 068 24 443 3 051 18 332 3 051 17 110 111 4 797 16 Plovdiv 6 321 63 498 4 741 47 624 4 741 44 449 6 321 17 268 17 Razgrad 2 820 18 435 2 115 13 826 2 115 12 905 2 820 2 838 18 Russe 1 528 14 876 1 146 11 157 1 146 10 413 1 528 1 523 19 Silistra 3 072 12 508 2 304 9 381 2 304 8 756 142 1 488 20 Sliven 1 981 30 326 1 486 22 745 1 486 21 228 173 7 699 21 Smolyan 8 874 15 254 6 656 11 441 6 656 10 678 204 604 22 Sofia town 395 4 121 296 3 091 296 2 885 376 1 383 23 Sofia-district 5 963 16 822 4 472 12 617 4 472 11 775 55 2 075 24 Stara Zagora 2 950 29 272 2 213 21 954 2 213 20 490 195 2 655 25 Targovishte 6 366 25 135 4 775 18 851 4 775 17 595 85 1 570

12 26 Haskovo 7 230 35 924 5 423 26 943 5 423 25 147 125 2 047 27 Shumen 4 544 24 225 3 408 18 169 3 408 16 958 176 5 928 28 Yambol 1 263 23 130 947 17 348 947 16 191 130 2 712 Total 106 970 588 784 80 232 441 591 80 232 412 150 15 894 84 753 b) Clinical examinations for the health status of sheep and goat in the framework of the program for control of Brucellosis.

Out of the FMD outbreaks in Jan – Apr 2011 in Burgas region, no evidence of FMD have been detected in sheep and goats during clinical examinations of their health status in 2011 and 2012 (as by end of June) on the whole territory of Bulgaria in the framework of the control program Brucellosis (tables 3 and 4).

Table 3: Clinical examinations of sheep and goats in 2011 without detecting any evidence for FMD

Number of clinical examinations in the Total number of existing framework of the control Region sheep and goats program for brucellosis in sheep and goats herds animals herds animals 1 Blagoevgrad 11 399 147 012 11399 90 738 2 Burgas 9 503 170 509 9503 22 266 3 Varna 11 397 78 908 11397 15 866 4 Veliko Tarnovo 11 414 88 745 11414 15 301 5 Vidin 9 361 43 398 9361 14 130 6 Vratsa 11 339 88 100 11339 14 559 7 Gabrovo 3 936 25 625 3936 4 867 8 Dobrich 8 478 98 622 8478 16 956 9 Kardjali 1 757 85 150 1757 28 959 10 Kjustendil 5 406 35 179 5406 12 901 11 Lovech 6 177 58 578 6177 7 703 12 Montana 14 551 79 511 14551 23 456 13 Pazardjik 5 565 62 469 5565 10 336 14 Pernik 3 224 27 609 3224 4 368 15 Pleven 13 179 85 669 13179 10 457 16 Plovdiv 5 021 140 683 5021 23 383 17 Razgrad 6 199 47 788 6199 11 396 18 Russe 6 502 51 724 6502 12 818 19 Silistra 8 464 65 066 8464 13 944 20 Sliven 5 518 90 847 5518 28 811 21 Smolyan 3 430 31 260 3430 14 041 22 Sofia town 350 13 432 350 1 395 23 Sofia-district 7 931 84 680 7931 22 882 24 Stara Zagora 8 990 61 218 8990 42 671 25 Targovishte 10 632 95 178 10632 19 804 26 Haskovo 5 699 111 544 5699 38 856

13 27 Shumen 10 496 98 673 10496 28 289 28 Yambol 7 626 80 081 7626 26 495 Total 213 544 2 147 258 213 544 577 648

Table 4: Clinical examinations of sheep and goats in 2012 (by end of June) without detecting any evidence for FMD

Number of clinical examinations in the Total number of existing framework of the control sheep and goats Region program for brucellosis in sheep and goats herds animals herds animals 1 Blagoevgrad 10 952 143 699 7 666 58 980 2 Burgas 8 857 202 110 6 200 14 473 3 Varna 10 869 71 650 7 608 10 313 4 Veliko Tarnovo 10 461 82 087 7 323 9 946 5 Vidin 9 217 36 522 6 452 9 185 6 Vratsa 10 951 85 466 7 666 9 463 7 Gabrovo 3 625 25 100 2 538 3 164 8 Dobrich 7 584 94 825 5 309 11 021 9 Kardjali 1 731 86 654 1 212 18 823 10 Kjustendil 5 206 32 132 3 644 8 386 11 Lovech 5 753 49 504 4 027 5 007 12 Montana 14 243 69 543 9 970 15 246 13 Pazardjik 5 524 62 351 3 867 6 718 14 Pernik 2 595 25 884 1 817 2 839 15 Pleven 12 076 80 957 8 453 6 797 16 Plovdiv 3 952 110 298 2 766 15 199 17 Razgrad 6 314 48 481 4 420 7 407 18 Russe 6 367 50 474 4 457 8 332 19 Silistra 7 881 65 206 5 517 9 064 20 Sliven 4 928 86 137 3 450 18 727 21 Smolyan 3 501 33 355 2 451 9 127 22 Sofia town 326 9 696 228 907 23 Sofia-district 7 989 74 658 5 592 14 873 24 Stara Zagora 8 567 59 885 5 997 27 736 25 Targovishte 10 632 95 178 7 442 12 873 26 Haskovo 5 875 114 106 4 113 25 256 27 Shumen 10 423 101 442 7 296 18 388 28 Yambol 7 264 107 307 5 085 17 222 Total 203 663 2 104 707 142 566 375 472 c) Clinical examinations of the health status of pigs in the framework of the Classical swine fever (CSF) control program

Out of the FMD outbreaks in Jan – Apr 2011 in Burgas region, no evidence of FMD have been detected in pigs during clinical examinations of their health status in 2011 and 2012

14 (as by end of June) on the whole territory of Bulgaria in the framework of the control program CSF (tables 5 and 6).

Table 5: Clinical examinations of pig herds in 2011 without detecting any evidence for FMD

Total number of Number of clinically Region (1) existing pigs examined herds animals herds 1 Blagoevgrad 3 213 7 480 3 213 2 Burgas 835 36 130 129 3 Varna 381 23 385 75 4 Veliko Tarnovo 1 843 31 420 656 5 Vidin 3 901 6 727 3 896 6 Vratsa 2 801 4 322 579 7 Gabrovo 811 4 913 758 8 Dobrich 1 650 16 997 1 144 9 Kardjali 11 378 4 10 Kjustendil 3 014 5 175 2 996 11 Lovech 618 5 805 486 12 Montana 1 684 9 035 1 366 13 Pazardjik 979 24 029 979 14 Pernik 911 1 440 1 15 Pleven 1 350 2 839 2 976 16 Plovdiv 596 5 124 556 17 Razgrad 36 37 117 21 18 Russe 166 93 085 118 19 Silistra 1 102 24 530 961 20 Sliven 1 791 12 468 40 21 Smolyan 72 469 18 22 Sofia town 297 1 370 229 23 Sofia-district 2 273 9 263 2 273 24 Stara Zagora 5 069 83 863 5 069 25 Targovishte 235 14 981 71 26 Haskovo 2 944 6 382 14 27 Shumen 904 57 601 904 28 Yambol 2 757 30 425 7 Total 42 244 556 753 29 539

Table 6: Clinical examinations of pig herds in 2012 (by end of June) without detecting any evidence for FMD

Total number of Number of clinically Region (1) existing pigs examined herds animals herds

15 1 Blagoevgrad 3 100 7 217 2 114 2 Burgas 835 36 130 194 3 Varna 381 23 385 238 4 Veliko Tarnovo 1 843 31 420 218 5 Vidin 3 901 6 727 2 720 6 Vratsa 2 801 4 322 339 7 Gabrovo 728 3 696 462 8 Dobrich 991 4 640 412 9 Kardjali 11 378 9 10 Kjustendil 3 014 5 175 3 014 11 Lovech 618 5 805 385 12 Montana 1 684 9 035 1 684 13 Pazardjik 160 19 879 8 14 Pernik 911 1 440 345 15 Pleven 1 350 2 839 849 16 Plovdiv 596 5 124 250 17 Razgrad 36 37 117 10 18 Russe 31 86 644 18 19 Silistra 852 12 332 16 20 Sliven 1 791 12 468 25 21 Smolyan 16 131 9 22 Sofia town 143 1 074 165 23 Sofia-district 2 110 10 702 938 24 Stara Zagora 3 613 92 853 525 25 Targovishte 235 14 981 235 26 Haskovo 2 755 6 820 116 27 Shumen 770 58 947 770 28 Yambol 2 757 30 425 2 111 Total 38 033 531 706 18 179 d) Clinical examinations of FMD susceptible livestock before issuing veterinary certificate in case of animal movements

No evidence of FMD have been detected in susceptible livestock during clinical examinations of the health status of the animals before issuing veterinary health certificate in case of animal movements on the whole territory of Bulgaria in 2011 and 2012 (as by end of June) (tables 7 and 8).

Table 7: Number (n) of FMD susceptible livestock inspected before issuing veterinary health certificate in case of animal movements in 2011

Region Cattle (n) Sheep & goats (n) Swine (n) 1 Blagoevgrad 9 624 17 884 659 2 Burgas 2 134 10 747 98 669 3 Varna 1 539 4 888 108 572 4 Veliko Tarnovo 5 482 20 872 41 120 5 Vidin 1 738 2 735 5 471 6 Vratsa 2 576 5 604 437 7 Gabrovo 1 995 7 172 4 648

16 8 Dobrich 5 561 13 515 27 168 9 Kardjali 1 115 2 190 0 10 Kjustendil 727 10 154 53 11 Lovech 5 473 16 649 10 376 12 Montana 3 898 5 226 3 956 13 Pazardjik 1 866 6 732 39 436 14 Pernik 597 742 8 15 Pleven 4 182 10 013 4 260 16 Plovdiv 16 906 46 867 6 121 17 Razgrad 3 105 12 187 53 109 18 Ruse 2 820 4 968 210 396 19 Silistra 1 689 21 179 11 576 20 Sliven 5 486 12 697 13 604 21 Smolyan 871 7 203 29 22 Sofia town 2 299 8 205 8 232 23 Sofia District 873 5 967 348 24 Stara Zagora 7 135 12 509 144 164 25 Targovishte 6 212 30 789 26 955 26 Haskovo 3 462 12 492 3 269 27 Shumen 3 245 9 363 115 898 28 Yambol 2 935 16 519 39 850 Total 104 430 336 068 978 384

Table 8: Number (n) of FMD susceptible livestock inspected before issuing veterinary health certificate in case of animal movements during the period 1st Jan – 30th June 2012

Region Cattle (n) Sheep & goats (n) Swine (n) 1 Blagoevgrad 4 470 15 029 351 2 Burgas 1 772 20 914 51 690 3 Varna 1 164 4 187 58 400 4 Veliko Tarnovo 2 285 7 768 18 996 5 Vidin 965 1 367 2 308 6 Vratsa 1 732 5 335 14 7 Gabrovo 933 3 268 2 110 8 Dobrich 2 967 8 971 12 615 9 Kardjali 1 897 8 198 0 10 Kjustendil 913 2 263 15 11 Lovech 2 239 7 016 5 549 12 Montana 1 948 2 399 898 13 Pazardjik 1 493 3 211 23 409 14 Pernik 415 645 0 15 Pleven 2 810 7 602 844 16 Plovdiv 10 704 22 940 3 436 17 Razgrad 2 227 13 810 20 407 18 Ruse 2 098 4 372 83 799 19 Silistra 1 132 17 819 6 480 20 Sliven 4 949 15 838 5 116 21 Smolyan 383 3 544 0 22 Sofia town 1 062 3 477 6 255 23 Sofia District 890 2 254 140 24 Stara Zagora 3 668 7 179 55 400 25 Targovishte 3 282 29 647 12 115

17 26 Haskovo 2 942 10 379 1 772 27 Shumen 2 981 13 789 60 641 28 Yambol 2 122 7 985 16 424 Total 66 443 248 943 449 184 e) Clinical examinations of FMD susceptible livestock at slaughterhouses in 2011 and 2012

No clinical signs or lesions suggesting FMD have been observed at slaughterhouses on the whole territory of Bulgaria during 2011 and 2012 (tables 9 and 10).

Table 9: Number (n) of FMD susceptible livestock inspected in slaughterhouses in Bulgaria in 2011

Region Cattle (n) Sheep & goats (n) Swine (n) 1 Blagoevgrad 575 567 6953 2 Burgas 0 2497 65 3 Varna 970 2980 24309 4 Veliko Tarnovo 84 277228 39082 5 Vidin 0 132 2664 6 Vratsa 1216 1083 1353 7 Gabrovo 3980 1063 21190 8 Dobrich 512 393 15577 9 Kardjali 3143 10069 13252 10 Kjustendil 164 717 4928 11 Lovech 866 954 9537 12 Montana 492 372 937 13 Pazardjik 247 1289 47051 14 Pernik 0 0 0 15 Pleven 219 416 65879 16 Plovdiv 6736 9806 140328 17 Razgrad 0 0 20 18 Ruse 153 0 39112 19 Silistra 750 9043 32311 20 Sliven 1921 3375 35755 21 Smolyan 0 0 0 22 Sofia town 0 6814 2286 23 Sofia District 0 8 19244 24 Stara Zagora 53 299 11377 25 Targovishte 1620 3634 115526 26 Haskovo 60 1279 24350 27 Shumen 325 2358 20557 28 Yambol 0 4255 13086 Total 24086 340631 706729

Note: The table includes data for inspected and slaughtered in slaughterhouses large and small ruminants and swine originating form all 28 regions of Bulgaria, as well as of animals imported for slaughter from EU countries. For each region is indicated the number of animals slaughtered in slaughterhouse located in the region – the inspected animals can originate from other regions of the country or EU.

18 Table 10: Number (n) of FMD susceptible livestock inspected in slaughterhouses in Bulgaria during 1st Jan – 30th June 2012

Region Cattle (n) Sheep & goats (n) Swine (n) 1 Blagoevgrad 478 3509 4066 2 Burgas 0 0 0 3 Varna 410 1759 13802 4 Veliko Tarnovo 0 69599 11298 5 Vidin 0 0 1788 6 Vratsa 671 652 227 7 Gabrovo 2134 2321 9838 8 Dobrich 333 3776 1776 9 Kardjali 1791 3662 8389 10 Kjustendil 121 1153 3788 11 Lovech 373 944 5878 12 Montana 236 229 265 13 Pazardjik 146 1772 29848 14 Pernik 0 0 0 15 Pleven 143 457 42101 16 Plovdiv 3044 6255 60256 17 Razgrad 0 0 0 18 Ruse 101 35 24071 19 Silistra 254 2311 18628 20 Sliven 929 2797 23454 21 Smolyan 0 0 0 22 Sofia town 0 15443 633 23 Sofia District 0 42 8948 24 Stara Zagora 34 70 5632 25 Targovishte 711 3047 62964 26 Haskovo 45 2014 11614 27 Shumen 120 135 5528 28 Yambol 0 3638 7435 Total 12074 125620 362227