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SYNJ2 rabbit pAb Catalog No : YT7593 Reactivity : Human, Mouse,Rat Applications : WB Gene Name : SYNJ2 KIAA0348 Protein Name : SYNJ2 Human Gene Id : 8871 Human Swiss Prot O15056 No : Mouse Gene Id : 20975 Mouse Swiss Prot Q9D2G5 No : Rat Gene Id : 84018 Rat Swiss Prot No : O55207 Immunogen : Synthesized peptide derived from human SYNJ2 Specificity : This antibody detects endogenous levels of SYNJ2 at Human/Mouse/Rat Formulation : Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. Source : Rabbit Dilution : WB 1：500-2000 Purification : The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. Concentration : 1 mg/ml 1 / 2 Storage Stability : -20°C/1 year Molecularweight : 164560 Background : The gene is a member of the inositol-polyphosphate 5-phosphatase family. The encoded protein interacts with the ras-related C3 botulinum toxin substrate 1, which causes translocation of the encoded protein to the plasma membrane where it inhibits clathrin-mediated endocytosis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2010], Function : alternative products:Additional isoforms seem to exist,catalytic activity:1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H(2)O = 1-phosphatidyl-1D-myo-inositol 4-phosphate + phosphate.,domain:The C- terminal proline-rich region of isoform 2B2 mediates binding to a variety of SH3 domain-containing proteins including SH3GL1, SH3GL2, SH3GL3 and GRB2.,function:Inositol 5-phosphatase which may be involved in distinct membrane trafficking and signal transduction pathways. May mediate the inhibitory effect of Rac1 on endocytosis.,similarity:Belongs to the synaptojanin family.,similarity:Contains 1 RRM (RNA recognition motif) domain.,similarity:Contains 1 SAC domain.,similarity:In the central section; belongs to the inositol-1,4,5-trisphosphate 5-phosphatase family.,subcellular location:Predominantly associated with the particulate fractions.,subunit:Binds to GRB2. Isoform 2A binds to SYNJ2BP/ Subcellular cytosol,cytoskeleton,plasma membrane,axon,membrane raft, Location : Products Images Western blot analysis of lysates from 293T cells, primary antibody was diluted at 1:1000, 4°over night 2 / 2 Powered by TCPDF (www.tcpdf.org).

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Phosphorylation of Synaptojanin Differentially Regulates Endocytosis of Functionally Distinct Synaptic Vesicle Pools
8882 • The Journal of Neuroscience, August 24, 2016 • 36(34):8882–8894 Cellular/Molecular Phosphorylation of Synaptojanin Differentially Regulates Endocytosis of Functionally Distinct Synaptic Vesicle Pools X Junhua Geng,1* Liping Wang,1,2* Joo Yeun Lee,1,4 XChun-Kan Chen,1 and Karen T. Chang1,3,4 1Zilkha Neurogenetic Institute, 2Department of Biochemistry and Molecular Biology, and 3Department of Cell and Neurobiology, Keck School of Medicine, University of Southern California, Los Angeles, California 90089, and 4Neuroscience Graduate Program, University of Southern California, Los Angeles, California 90089 The rapid replenishment of synaptic vesicles through endocytosis is crucial for sustaining synaptic transmission during intense neuronal activity. Synaptojanin (Synj), a phosphoinositide phosphatase, is known to play an important role in vesicle recycling by promoting the uncoating of clathrin following synaptic vesicle uptake. Synj has been shown to be a substrate of the minibrain (Mnb) kinase, a fly homolog of the dual-specificity tyrosine phosphorylation-regulated kinase 1A (DYRK1A); however, the functional impacts of Synj phosphorylation by Mnb are not well understood. Here we identify that Mnb phosphorylates Synj at S1029 in Drosophila. We find that phosphorylation of Synj at S1029 enhances Synj phosphatase activity, alters interaction between Synj and endophilin, and promotes efficient endocytosis of the active cycling vesicle pool (also referred to as exo-endo cycling pool) at the expense of reserve pool vesicle endocytosis. Dephosphorylated Synj, on the other hand, is deficient in the endocytosis of the active recycling pool vesicles but maintains reserve pool vesicle endocytosis to restore total vesicle pool size and sustain synaptic transmission. Together, our findings reveal a novel role for Synj in modulating reserve pool vesicle endocytosis and further indicate that dynamic phosphorylation and dephosphorylation of Synj differentially maintain endocytosis of distinct functional synaptic vesicle pools.
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SYNJ2 Antibody (Monoclonal) (M02) Mouse Monoclonal Antibody Raised Against a Partial Recombinant SYNJ2
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