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AMC 2019 Poster Presentation 1 P1-25 Behavior of Hymenoscyphus fraxineus in the leaves of four foreign ash species (Fraxinus spp.) in Hokkaido, Japan Saori Kato1), Izumi Okane2), Yutaka Tamai3), Takehiro Yamaguchi4), Yuichi Yamaoka2), Yasuhiro Ishiga2) 1)Graduate School of Life and Environmental Sciences, University of Tsukuba, Japan 2)Faculty of Life and Environmental Sciences, University of Tsukuba, Japan 3)Research Faculty of Agriculture, Hokkaido University, Japan 4)Hokkaido Research Center, Forestry and Forest Products Research Institute, Japan Purpose: Hymenoscyphus fraxineus causes a lethal disease known as “ash dieback" in European ash Fraxinus excelsior, in Europe. However, this fungus endophytically inhabits the living leaves of Manchurian ash, F. mandshurica, in Japan. Hymenoscyphus fraxineus can infect ashes including East Asian and Euramerican species. While F. excelsior is susceptible in general, this species are healthily growing in the Sapporo campus of Hokkaido University. Fraxinus pennsylvanica and F. americana are economically important in North America, concerned attacks by H. fraxineus possibly introduced. In this study, we compared the behavior of H. fraxineus in the leaves of foreign ash species. Methods: Four ash trees, F. excelsior, F. pennsylvanica, F. americana (Euramerican species) and F. chinensis subsp. rhynchophylla (East Asian species), growing in Sapporo were investigated. Fraxinus pennsylvanica and F. americana are weakening in the sites. Symptomless leaves of the trees were continuously collected. Whole DNA were extracted from the leaf samples, and then, the relative amount of fungal DNA of H. fraxineus to plant DNA was calculated by using real-time quantitative polymerase chain reaction. Results and conclusions: The fungal DNA was apparently lower in F. excelsior healthily growing in the site. In F. pennsylvanica, the fungal DNA began to increase at the onset of the defoliation period. That in the leaflets of F. americana reached the peak even in late August. In F. chinensis subsp. rhynchophylla, the fungal DNA was slightly detected and retained low. These results suggested that change of relative amount of fungal DNA in the leaves was different between four ashes. Asian Mycological Congress 2019.

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