bioRxiv preprint doi: https://doi.org/10.1101/2021.06.17.448907; this version posted June 18, 2021. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY 4.0 International license. 1 Whole-genome sequencing and analysis of two azaleas, Rhododendron ripense and Rhododendron 2 kiyosumense 3 4 Kenta Shirasawa1*, Nobuo Kobayashi2, Akira Nakatsuka2, Hideya Ohta2, Sachiko Isobe1 5 6 1Kazusa DNA Research Institute, 2-6-7 Kazusa-Kamatari, Kisarazu, Chiba 292-0818, Japan 7 2Faculty of Life and Environmental Science, Shimane University, 1060 Nishikawatsu, Matsue, Shimane 8 690-8504, Japan 9 10 *To whom correspondence should be addressed: 11 Kenta Shirasawa 12 2-6-7 Kazusa-Kamatari, Kisarazu, Chiba 292-0818, Japan 13 Tel.: +81 438 52 3935 14 Fax: +81 438 52 3934 15 E-mail:
[email protected] 16 17 Running title: Whole-genome sequencing of two azalea genomes 18 1 bioRxiv preprint doi: https://doi.org/10.1101/2021.06.17.448907; this version posted June 18, 2021. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY 4.0 International license. 19 ABSTRACT 20 To enhance the genomics and genetics of azalea, the whole-genome sequences of two species of 21 Rhododendron were determined and analyzed in this study: Rhododendron ripense, the cytoplasmic donor 22 and ancestral species of large-flowered and evergreen azalea cultivars, respectively; and Rhododendron 23 kiyosumense, a native of Chiba prefecture (Japan) seldomly bred and cultivated.