(12) Patent Application Publication (10) Pub. No.: US 2008/0175934 A1 Mitra Et Al

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(12) Patent Application Publication (10) Pub. No.: US 2008/0175934 A1 Mitra Et Al US 2008O175934A1 (19) United States (12) Patent Application Publication (10) Pub. No.: US 2008/0175934 A1 Mitra et al. (43) Pub. Date: Jul. 24, 2008 (54) NOVEL HERBAL COMPOSITION OF (21) Appl. No.: 11/965,239 EXTRACTS OF QUERCUS INFECTORIA, PROCESS FOR PREPARING THE SAME AND (22) Filed: Dec. 27, 2007 USE THEREOF (30) Foreign Application Priority Data (75) Inventors: Shankar Kumar Mitra, Bangalore Dec. 29, 2006 (IN) ........................... 2831 (DELA2006 (IN); Ekta Saxena, Bangalore (IN); s Rangesh Paramesh, Bangalore Publication Classification (IN); Uddagiri Venkanna Babu, (51) Int. Cl Bangalore (IN); Sundaram we Ramachandran, Bangalore (IN); A6IR 36/49 (2006.01) Marikunte Venkata Ranganna, (52) U.S. Cl. ........................................................ 424/771 Bangalore (IN) (57) ABSTRACT Correspondence Address: Disclosed is a novel herbal composition comprising extract of DARBY & DARBY P.C. Quercus infectoria having rich and effective concentration of P.O. BOX 770, Church Street Station as antioxidants and phenolic compounds. Further the present New York, NY 10008-0770 invention provides a process for preparing said composition and use thereof in cosmeceuticals, pharmaceuticals and (73) Assignee: Himalaya Global Holdings health drinks for treating oxidative stress and other life style Limited, Grand Cayman, KY (US) diseases. Patent Application Publication Jul. 24, 2008 Sheet 1 of 10 US 2008/O175934 A1 40 33 19 20 1 8 STD AXT-01 AXT-O2 AXT-16 AXT-17 AXT-18 STD = BHT Fig.1. US 2008/O175934 A1 AXT-15 ÅXT-34 ÅXT-24 STD = BHT Fig.2. Patent Application Publication Jul. 24, 2008 Sheet 3 of 10 US 2008/O175934 A1 33.) STD AXT. AXT-3 STDBHT Fig.3. Patent Application Publication Jul. 24, 2008 Sheet 4 of 10 US 2008/O175934 A1 40 33. STD AXT-9 AXT. STEBHT Fig.4. Patent Application Publication Jul. 24, 2008 Sheet 5 of 10 US 2008/O175934 A1 s . O.5 0.4 STD AXT-1 AXT AXT-6 AXT-1, AXT-8 STD Wit Fig.5. Patent Application Publication Jul. 24, 2008 Sheet 7 of 10 US 2008/0175934 A1 2. 5 E 1.5 C 5 1 0.5 STD AXT-22 AXT-23 STD = Wit-C Fig.7. Patent Application Publication Jul. 24, 2008 Sheet 8 of 10 US 2008/O175934 A1 STD AXT-9 AXT-2) STD = Wit-C. Fig.8. Patent Application Publication Jul. 24, 2008 Sheet 9 of 10 US 2008/O175934 A1 &O) 3.456 3O) E S. E 2000 e 1000 73 73 f 152 Wit-C. AXT-01 AXT-3 ATX-10 AXT-31 AXT-33 Fig.9. Patent Application Publication Jul. 24, 2008 Sheet 10 of 10 US 2008/O175934 A1 r l 1.6 6 lik 15 ld O I {Quercetin Fig.10. US 2008/01 75934 A1 Jul. 24, 2008 NOVEL, HERBAL COMPOSITION OF radicals and also the overall composition should improve the EXTRACTS OF QUERCUS INFECTORIA, bioavailability of the antioxidant active fraction. PROCESS FOR PREPARING THE SAME AND USE THEREOF PRIOR ART 0009 U.S. Pat. No. 6,534,046 to Golz-Bemer et al. dis FIELD OF THE INVENTION closed the preparation of plant derived anti-perspiration cos metics comprising the extracts of Equisetum arvense, Salvia 0001. This invention, in general relates to a novel herbal officinalis, Hamamelis Virginia and Quercus infectoria. composition comprising extracts of Quercus infectoria. More (0010 U.S. Pat. No. 6,264,926 to Farooqi et al. disclose a particularly, the present invention provides a novel herbal formulation useful as a natural herbal tooth powder compris composition comprising extract of Quercus infectoria having ing the extracts of Quercus infectoria, Zanthoxylum amatum high and effective concentration of antioxidants and phenolic and Zingiber officinalis etc. (0011 Kaur G. et al. (J. Ethnopharmacol. 2004, 90(2-3). compounds. The invention further provides a process for 285-92) have reported the anti-inflammatory activities of preparing said composition and use thereof. alcoholic extracts of galls of Quercus infectoria. 0012 Sawangiaroen N et al (J Ethnopharmacol 2005 BACKGROUND OF THE INVENTION 98(1-2) 67-72) have reported the antidiarrhoel effect of methanol extract of galls of Ouercus infectoria. 0002 Reactive Oxygen species and Reactive Nitrogen (0013 United States Patent application No. 20030138509 species (ROS/RNS) arise in the normal course of oxidative to Pushpangadan et al. teaches the preparation of fermented metabolism. These reactive species (ROS/RNS) influence herbal health drink from the plant Andrographis paniculata. multiple metabolic, signaling and transcriptional processes, 0014 U.S. Pat. No. 4,741,915 to Farret al. disclosed the which are involved in the normal function of cell. use of purified and hydrolyzed gallotannins derived from 0003 Oxidative stress is a condition that occurs when plant materials for inhibition of oxidation in foodstuffs. there is excess and prolonged imbalance between the genera 0015 U.S. Pat. No. 7,041,332 to Gaudout et al. teaches the tion of reactive oxygen species and reactive nitrogen species preparation of a phenolic rich fraction obtained from the fruit and control by antioxidants. Reducing reactive oxygen spe of family rosaceae for use in cosmetic, dietary and nutraceu cies and reactive nitrogen species in the body is one of the tical preparation. primary target towards the protection of various diseases such 10016 U.S. Pat. No. 6,989,161 to Roufetal discloses a as aging process, cancer, diabetes and neurodegenerative dis composition comprising phytonutrients viz. lutein, lycopene, CaSCS. epigallocatechingallate (EGCG), ellagic acid, hesperidin and 0004 Dietary compounds such as vitamins C and E may quercetin. limit oxidative damage directly but along these other dietary constituents viz. carotenoids and polyphenols may also act SUMMARY OF THE INVENTION via indirect mechanisms such as endogenous antioxidant 0017. It is a principal object of the present invention to enzyme activity and thereby reduce the risk of a number of the provide a herbal composition comprising extract of Quercus age related disorders. infectoria having high and effective concentration of antioxi 0005 Polyphenols occur most commonly in foods of plant dant and phenolic compounds. origin have attracted much attention as potent antioxidants 0018. Another aspect of the present invention is to obtain due to their ability to scavenge free radical and form inert the extract using all parts of the plant Quercus Infectoria, phenoxy radical intermediates. A number of epidemiological preferably from the galls. studies have Suggested that consumption of polyphenol-rich 0019. Yet another aspect of the present invention is to foods reduces the risk of developing chronic diseases. obtain a herbal composition comprising antioxidant rich 0006 Although, these polyphenol antioxidants reported extract of Quercus Infectoria, wherein the antioxidant is pref for the prevention of various lifestyle diseases but only a few erably methyl gallate. findings show that they are active and majority of the results 0020 Still another aspect of the present invention is to show that they have no clinical efficacy. The vast literature on obtain the extract of Ouercus Infectoria employing solvents antioxidants Suggests that the failure of polyphenol antioxi selected from non-polar hydrocarbon, chlorinated Solvents, dant in clinical cases because it is almost impossible to elimi an ester and ketones, an alcohol and water, preferably, nate many active oxygen species produced in the body by selected from hexane, chloroform, ethyl acetate, acetone, single antioxidant molecule. methanol or ethyl alcohol. 0007 Polyphenol antioxidants derived from the plant ori 0021. Yet another aspect of the present invention is to gin have structurally phenol group as backbone and make it obtain the extract of the said herbal composition employing more lipid soluble. The bioavailability of these plant polyphe methanol with hexane and acetone preferably in 1:1 ratio, nols is very low in GI tract and therefore most of these wherein the said extract is characterized by having antioxi antioxidants have not sufficiently displayed efficacy in the dant concentration of more than 80%. body even though they have been reported as potent antioxi 0022. Still another aspect of the present invention is to dants in vitro. obtain the extract of the said herbal composition employing 0008 To overcome the above said difficulties in the prepa methanol with chloroform and methanol preferably in 1:1 ration of antioxidant composition that is effective both in ratio, wherein said extract is characterized by having phenolic vitro and clinical, it is necessary to select a group of polyphe compounds concentration of more than about 108 w/w nolic compounds rich composition that is selectively address 0023. In accordance with further aspect of the present the inhibition of many enzymatic pathways that produce free invention, there is provided an antioxidant and phenolic com US 2008/01 75934 A1 Jul. 24, 2008 pounds rich herbal composition, which is obtained by a pro yeast Malassezia furfur (MTCC-1374) causing dandruff and cess, comprising the steps of (a) pulverizing the shade dried Malassezia pachydermatis causing ear infection. galls of Quercus Infectoria to coarse powder, (b) Subjecting 0033. In one another preferred aspect of the present inven the resultant coarse powder of said dried galls of Quercus tion is to evaluate the antioxidant and free radical scavenging Infectoria into an extraction vessel in presence of a solvent activity of the said herbal composition by employing DPPH either alone or in combination thereof, (c) filtering the result method, Ferric reducing Power Assay Method, and determin ant plant extracts employing Suitable filter, (d) drying the ing Nitric oxide scavenging activity. resultant solvent extracts to form a concentrate. 0034. Yet another aspect of the present invention is to 0024. Further aspect of the present invention is the prepare the extracts of Quercus Infectoria by employing per obtained extract is characterized by having antioxidant prin colation method and hot soxhlet method. ciple concentration of more than 80% and phenolic principle 0035 Another aspect of the present invention is to prepare concentration of more than about 108% w/w.
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