Studies on the Immature Stages of Oxya Velox (Fabricius) a Rice Grasshopper from District Jamshoro, Sindh, Pakistan
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SULTANA ET AL (2012), FUUAST J. BIOL., 2(1): 57-62 STUDIES ON THE IMMATURE STAGES OF OXYA VELOX (FABRICIUS) A RICE GRASSHOPPER FROM DISTRICT JAMSHORO, SINDH, PAKISTAN RIFFAT SULTANA*, BARKAT ALI BUGHIO, WAHEED ALI AND HAJI KHAN Department of Zoology, University of Sindh, Jamshoro *Corresponding author e-mail: [email protected] Abstract Grasshoppers are polyphagous insect pest. They cause considerable damage to many precious crops. Amongst these pest species Oxya is reported as severe pest to rice in many countries of world including Pakistan. The immature stages of Oxya velox (F.) has been studied in this paper. Oxya comprises on sixth nymphal instars. These hoppers appear to be more epidemic than the adults, because they have no functional wings and are unable to fly and all the time they eat; hence cause more damage than the adult. In addition to this, morphological description of various nymphal instars, identification key and measurements of different body parts are also provided. This will, hopefully be useful in plants protection. Introduction Rice is the world most important food crop second to wheat, feeding over 2 billion people in Asia alone (Karim and Riazuddin 1999). The rice plant is vulnerable to many insects from its sowing to harvest. Amongst the pests Oxya species (mostly called small grasshopper) are reported as sporadic pest of rice in all developmental stages i.e. seeding, tillering, stem elongation etc. Grasshopper at times, occurs in very large numbers. They have great mobility so they migrate from one place to another. Many grasshoppers’ species are occasionally found in shady fields but rarely cause significant damage other than along field borders (Irshad, 1977; Zafar; 1986). They chew angular holes in leaves causing an injury similar to that caused by leaf-fodder or Armyworm. Grasshoppers are polyphagous insect pest. The hoppers are seen to be more epidemic than the adults, because they have no functional wings, so are enable to fly and all the times they eat, hence cause more damage than the adults. Although, there is bulk of publications available on the food preference, plant selection, distribution pattern, status of this pest and other grasshoppers (Srodgram 1935; Dempster 1963; Uvarov 1966, 1977; Thakur 1984; Aziz and Aziz, 1985; Inayatullah et al., 1986; Mohan and Manoharan 1987; Shen et al., 1988; Premchand 1995, Lanjar et al., 2002 and Riffat and Wagan 2010) but there are several aspects regarding the biology of Oxya species are less known. It is, therefore, felt necessary to examine the immature stages of this pest. Actually the basic knowledge about the biology of pest species provides authentic information for its control. The results of such study will be instrumental in understanding and devising the population management strategies to adopt control measures at appropriate time. Materials and Methods Collection of samples: The nymphs of O.velox were collected from the agriculture fields of rice, fodder crops and their surrounding vegetation of grasses with the help of traditional insect hand-net (8.89 cms in diameter and 50.8 cms in length) as well as by hand picking. The collection was made during the year 2011 in the months (May-October) from various localities of Jamshoro (Map. I). The collected material was transferred into polythene bags and taken to the laboratory for further study. Killing and preservation of samples: The method was adopted from Vickery and Kevan (1983). The collected material brought in to the laboratory and was killed by means of potassium cyanide in standard entomological killing bottles. The specimens were not left too longer in this medium because the color changed particularly that of green specimens. Pinning of the specimens was made within few hours. As the specimens were flexible there was a little danger of losing any part through the necessary manipulation, further the parts could be stretched as desired. Mounting was done according to the following standard entomological. The insect pins were inserted on the pronotum posterior to transverse sulcus slightly to the right of median dorsal carina. The posterior legs were bent beneath the body to minimize the possibility of breaking and to occupy the least amount of storage space. The abdomen was so set that it dropped below the wings and not obscured by the hind legs as several taxonomic characters are found on the terminal end and these were not to be hidden till the specimens were dried thoroughly. The body parts had to be supported with extra pins so that it can dry in the desired position and also special attention was paid to the antennae, wings and legs in order to display important taxonomic SULTANA ET AL (2012), FUUAST J. BIOL., 2(1): 57-62 58 characters. Dust and other extraneous matter were removed with the help of a dry camel hairbrush. The fully dried specimens were removed from stretching boards and were stored in standard entomological boxes with labels showing locality, date of collection and collector name. Naphthalene balls were placed in boxes to prevent the attack of ants and other insect. Identification & measurements of hopper: Identification of hopper was carried out under the stereoscopic dissecting binocular microscope. The body parts of the hopers were measured with vernier caliper except first and second instars and antennae of other instars .The first and second instar hopper and antennae were measured by ocular squire graph and segment of an antenna were counted under microscopic. All the measurements are given in millimeter (mm). Results Key to the nymphal instars of Oxya velox (F.) 1. Elytron and wing rudiments, when present directed downward ……..…………..2 --Elytron and wing-rudiments turned upward…………….….. …….………..…..5 2. Elytron and wing rudiments not developed, ♂antennal length 2.25±0.10mm with 2.25±2.47 segments (Fig.1 a)…………..……………………First instar --Elytron and wing rudiments evident, ♂antennal length2.46±0.01 mm with usually 2.60± 0.14 segments…………....................................................................3 3. ♂Antennal length usually 2.36±0.014 mm with 2.65 segments, elytron and wing rudiments faintly marked directed downwards. (Fig.1 b) …………………………………………………………………….....Second instar --♂Antennal length 2.46±0.14 mm with 2.72 segments ……….…..….......................4 4. Elytron-and-wing-rudiments directed downward, slightly back, smooth to weak rugose (Fig.1 c)…………...........................................................................................Third instar --♂Antennal length 2.66mm with 2.8±0.14 segments, elytron-and-wing-rudiments directed downward and noticeably back, conspicuously rugose (Fig1.d) ……………….………………………………………………….……Fourth instar 5. Elytron and wing-rudiments not extending beyond first abdominal Segment (Fig1.e)……….…………………………………….…….Fifth instar 6. Elytron and wing-rudiments extending well beyond first abdominal Segment (Fig1.f)……………..……………… ……………….……Sixth instar a b c Ist instar 2nd Instar 3rd Instar d e f 4th Instar 5th Instar 6th Instar Fig. 1 (a-f) showing the different nymphal stages of Oxya velox SULTANA ET AL (2012), FUUAST J. BIOL., 2(1): 57-62 59 B S A M T K Map of district Jamshoro Showing Surveyed areas during present study B=Bhan Saeedabad; S=Sehwan; A=Amri; M=Manjhand; T=Thano Bula Khan; K=Kotri Morphological description of hopper stages First stage nymph: Body coloration light green pale, pronotum dark at posterior end. Head triangular, a little raised above pronotum, upper margin appears slightly curved in profile, vertex slightly produced and angular terminally. Compound eyes grayish in colour and speckled with brown pigments, elongate, oval, large sized, and prominently developed. Two small pits, one on either side of median carinae, Frontal carinae widely apart and somewhat divergent posterior, lateral and sub–colour carinae indistinctly developed. Pronotum broad and highly developed and overlies mesonotum dorsally and laterally; posterior–lateral areas of mesonotum exposed as small triangles. Median carinae conspicuous on pronotum faint on meso-and meta-nota. Pronotum transversally sulcated by four furrows, equally developed not reaching lateral margin; second sulcus shortest and fourth indistinct on dorsal aspect but developed on lateral sides. Tegminal and wing rudiments produced as margins of meso-and meta-nota. Pro-and mesolegs small having characteristic blackish spot frequently distributed on femora, tibia and tarsi bearing bluish appearance. Abdominal short, light greenish on dorsal side and polish on ventral aspect. Cerci well developed and bearing numerous setae on its surface Second stage nymph: Coloration as in first instar. Head deflected down words when seen in profile rather cute, vertex produced interiorly. Two white spots, one on each side, at junction of edges of vertex with compounds eyes. Compound eyes of bigger size appear somewhat separated from above. Two greenish lateral ocelli, a whitish median ocellus. Frontal carina bearing row dark pigmented spots along. Frons and clypeus bear numerous minute spots. Tips of maxillary palpi and antennae greenish, antennal tips light brown. A white dorsal median band running from the vertex to tip of abdomen. Pronotum well developed and having three distinct transverse sulci, anterior margin convex and posterior concave. Hind margins of all thoracic segments bear dark spots. Terminal and wing rudiments pushed postero-laterally acquire folded appearance. Femora robust; tarsi and tibia spines of all legs bear a light green pigmentation, tibiae black tipped.