Protein Power
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PROGRAMME AND ABSTRACT BOOK FROM PROTEOME TO PHENOTYPE: ROLE OF POST-TRANSLATIONAL MODIFICATIONS 11 – 13 DECEMBER 2017 UNIVERSITY OF EDINBURGH, UK PROTEIN POWER SOCIETY FOR EXPERIMENTAL BIOLOGY CELL SECTION SYMPOSIUM CONTENTS 01 FROM PROTEOME TO PHENOTYPE: ROLE OF POST-TRANSLATIONAL MODIFICATIONS 1. DELEGATE INFORMATION 02 2. PROGRAMME 03 3. POSTER SESSION 07 4. ABSTRACTS 14 5. POSTER ABSTRACTS 30 6. AUTHOR INDEX 63 ORGANISED BY: GERAINT PARRY GARNET STEVEN SPOEL UNIVERSITY OF EDINBURGH, UK CYRIL ZIPFEL THE SAINSBURY LABORATORY, UK IN COLLABORATION WITH: CELL SECTION SYMPOSIUM DELEGATE INFORMATION 02 CELL SECTION SYMPOSIUM PROGRAMME 03 DELEGATE PROGRAMME INFORMATION BADGES POSTER SESSION MONDAY 11 DECEMBER 11:45 John Christie Participants are required to wear name badges The poster session will be taking place in the University of Glasgow, United Kingdom at all times for proof of registration, security breakout area between 18:30 – 20:00 on Monday 09:00 REGISTRATION Engineering the function of Phototropin purposes and catering identification. 11 December 2017. Poster presenters are invited to receptor kinases hang their poster on their arrival (Velcro will be 09:25 CS17.4 provided) and are asked to remove their posters by Welcome CATERING 12:15 16:00 on Tuesday 12 December. Any posters left Cyril Zipfel Kris Vissenberg behind will be disposed of. Lunch and refreshments during the symposium Steven Spoel University of Antwerp, Belgium are included in your registration fee and will be Cell symposium co-organisers The auxin-regulated kinase ERULUS controls cell served in the breakout area. LIABILITY wall composition during root hair tip growth in 09:30 Arabidopsis thaliana PLENARY LECTURE Neither the Society for Experimental Biology nor the CS17.5 CERTIFICATE OF ATTENDANCE Jesper Olsen University of Edinburgh will accept responsibility University of Copenhagen, Denmark for damage or injury to persons or property during 12:30 Delegates requiring a certificate of attendance Dissecting cell signalling networks the symposium. Participants are advised to arrange Cornelia Klose should visit the SEB registration desk on by quantitative phosphoproteomics their own personal health and travel insurance. Institute of Biology 2, University of Freiburg, their departure. CS17.1 Germany Dynamic regulation of phytochrome B 10:15 REFRESHMENT BREAK/POSTERS VENUE PHOTOGRAPHY activity by phosphorylation SESSION 1 DYNAMIC PHOSPHORYLATION CS17.6 John McIntyre Conference Centre No photographs are to be taken of the speakers Edinburgh First, Pollock Halls and their slides during the symposium. CHAIR: CYRIL ZIPFEL 12:45 LUNCH/POSTERS 18 Holyrood Park Road, Edinburgh EH16 5AY *Please note: The SEB will be taking photos during the event for promotional purposes. If you have any 10.45 SESSION 2 MECHANISMS AND ACTION OF PROTEIN The scientific sessions will be taking place in concerns, please visit the SEB registration desk. Cyril Zipfel SUMOYLATION the Pentland room and the catering and posters The Sainsbury Laboratory, United Kingdom CHAIR: ARI SADANANDOM will be in the breakout area. REGISTRATION Phosphocode-dependent functional dichotomy of the common co-receptor BAK1 in plant signalling 14:00 CS17.2 GARNET WORKSHOP ON PLANT PROTEOMICS The registration desk will be open during the hours Ari Sadanandom of the symposium and a SEB staff member will be Durham University, United Kingdom If you have been selected to attend the plant on hand during the refreshment and lunch breaks 11:15 Rice SUMO protease Overly Tolerant to Salt 1 proteomics workshop on 13 December, you will should you require any assistance. Gitta Coaker targets the transcription factor, OsbZIP23 to have received further information by email. University of California Davis, United States promote drought tolerance in rice The PBL13 kinase negatively regulates the ROS CS17.7 Please visit the SEB registration desk if you TWITTER require information or have any queries. burst in the absence of pathogen perception CS17.3 14:30 We’re looking to increase the conversation at the Andreas Bachmair WI-FI INTERNET ACCESS meeting using Twitter so please get tweeting! Max F. Perutz Laboratories, University of Vienna, Follow the conversation #SEBPTM17 Dept. Biochemistry and Cell Biology, Austria Internet access is available during the symposium SEB – @SEBiology SUMO conjugation - chain formation and links and will be complimentary. Log in details will be GARNet – @GARNetweets to phosphorylation available at the registration desk. CS17.8 CELL SECTION SYMPOSIUM PROGRAMME 04 CELL SECTION SYMPOSIUM PROGRAMME 05 PROGRAMME PROGRAMME 15:00 17:30 TUESDAY 12 DECEMBER 11:45 L Maria Lois Stéphane Ravanel Barbara Korbei CRAG, Spain CEA, Plant Cell Physiology laboratory - University of Natural Resources and Life Sciences Deciphering SUMO conjugation in vivo: multiple Grenoble, France 08:45 REGISTRATION Vienna, Austria regulatory layers controlling plant growth Protein lysine methylation in plants: from proteome Ubiquitin binding and conjugation is important CS17.9 to phenotype…the quest for the Holy Grail 09:00 for the function and localisation of TOL proteins CS17.14 PLENARY LECTURE CS17.21 15:30 Ron Hay Mark Kwaaitaal 18:00 University of Dundee, United Kingdom 12:00 Molecular Plant Pathology, Swammerdam Doris Lucyshyn SUMO-SIM networks regulate the assembly Marco Trujillo Institute for Life Sciences, University of University of Natural Resources and Life of dynamic protein complexes Leibniz Institute for Plant Biochemistry, Germany Amsterdam, Netherlands Sciences Vienna, Austria CS17.17 Phosphorylation and ubiquitination regulate the SUMOylation orchestrates formation of nuclear O-Glycosylation of nuclear and cytosolic proteins Exo70B2 subunit of the exocyst – a convergence bodies that overlap with COP1 photobodies in plants 09:45 REFRESHMENT BREAK/POSTERS point of immune signalling and autophagy CS17.10 CS17.15 CS17.22 SESSION 4 UBIQUITIN-MEDIATED PROTEASOME SYSTEM 15:45 18:15 12:15 LUNCH/POSTERS Justyna Labuz Amanda Chaplin CHAIR: STEVEN SPOEL Jagiellonian University, Poland University of Essex, United Kingdom SESSION 5 REDOX-BASED PTMs IN STRESS AND DEVELOPMENT Sumoylation of phototropins in Glycated peptides triggers H2O2 signalling 10:15 Arabidopsis thaliana in Arabidopsis thaliana Steven Spoel CHAIR: GARY LOAKE CS17.11 CS17.16 University of Edinburgh, United Kingdom Transcriptional regulation by dynamic 13:30 16:00 REFRESHMENT BREAK/POSTERS 18:30 POSTER SESSION ubiquitination Gary Loake CS17.18 University of Edinburgh, United Kingdom SESSION 3 EMERGING PTMS SUMOYLATION 20:00 DINNER Control of plant immune function through LOCATION: SOUTH HALL COMPLEX 10:45 S-nitrosylation CHAIR: PIERS HEMSLEY Luz Irina Calderon-Villalobos CS17.23 Leibniz Institute of Plant Biochemistry (IPB), 16:30 Germany 14:00 Piers Hemsley Auxin-programmed ubiquitylation as a master Frank van Breusegem University of Dundee, United Kingdom signal integration system VIB Ghent, Belgium S-acylation in plants - greasing membrane CS17.19 Oxidative stress signalling in plants. protein function? Towards the proteome and beyond CS17.12 11:15 CS17.24 Emmanuelle Graciet 17:00 Maynooth University, Ireland 14:30 Markus Wirtz Regulation of plant/pathogen interactions Daniel Gibbs Heidelberg University, Germany by the N-end rule pathway University of Birmingham, United Kingdom N-terminal acetylation - a prevalent protein CS17.20 The N-end rule pathway couples PRC2 activity modification that likes to surprise to oxygen availability in flowering plants CS17.13 CS17.25 CELL SECTION SYMPOSIUM PROGRAMME 06 CELL SECTION SYMPOSIUM POSTER SESSION 07 PROGRAMME POSTER SESSION MONDAY 11 DECEMBER 15:00 17:00 Gregory MacNeill Marita Anggarani Eva-Esther Rudolf Katja Baerenfaller University of Guelph, Canada Institute of Plant Molecular Biology, Academia Helmholtz Zentrum München, Germany SIAF, Switzerland Post-translational modifications regulate Sinica, Taiwan Post-translational modification of histones: On-site inspection of ubiquitylation starch branching enzyme 2.2 in Arabidopsis Impaired proteasome function in Arabidopsis Nitric oxide modulates chromatin structure CS17.30 CS17.33 rad23abcd mutant alters auxin homeostasis CS17.26 CS17.39 17:30 Yang Do Choi 15:15 Greg Vert Seoul National University, Korea (South) Ram Nivas Ahirwar Capilla Mata-Perez I2BC CNRS, France OsSGT1 regulates root development and Institute of Plant Molecular Biology, Academia University of Edinburgh, United Kingdom Proteasome-independent roles of growth in rice Sinica, Taiwan Nucleoredoxin 1 (NRX1) selectively rescues lysine63-linked polyubiquitin chains: CS17.34 Arabidopsis deubiquitinase OTU5 is involved plant immunity Insight from sensor-based proteomics in flowering by regulating major repressors CS17.27 CS17.31 Mansoor Azeem Siddiqui FLC/MAF4/MAF5 International Centre for Genetic Engineering CS17.40 15:30 REFRESHMENT BREAK/POSTERS 17:45 and Bio-technology, India Ive De Smet Protein S-Palmitoylation regulates invasion Maura Di Martino TECHNOLOGICAL ADVANCES SESSION 6 VIB, Belgium and apical organelle secretion in human malaria University of Warwick, United Kingdom IN PTM DETECTION The dynamic phosphoproteome parasite, Plasmodium falciparum Combinatorial transcriptional regulation allows identifying novel abiotic stress CS17.35 of the plant defence response CHAIR: ALEX JONES signalling components CS17.41 CS17.32 Arsheed Sheikh 16:00 School of Life Sciences, University Jasmine Pham Alex Jones 18:00 END OF SYMPOSIUM of Warwick, United Kingdom The James Hutton Institute, United Kingdom