Usbiological Datasheet

PAPOLG, CT (PAPOLG, PAP2, PAPG, Poly(A) polymerase gamma, Neo-poly(A) polymerase, Polynucleotide adenylyltransferase gamma, SRP RNA 3'-adenylating enzyme) (AP) Catalog number 039644-AP Supplier United States Biological This gene encodes a member of the poly(A) polymerase family which catalyzes template- independent extension of the 3' end of a DNA/RNA strand. This enzyme shares 60% identity to the well characterized poly(A) polymerase II (PAPII) at the amino acid level. These two enzymes have similar organization of structural and functional domains. This enzyme is exclusively localized in the nucleus and exhibits both nonspecific and CPSF (cleavage and polyadenylation specificity factor)/AAUAAA-dependent polyadenylation activity. This gene is located on chromosome 2 in contrast to the PAPII gene, which is located on chromosome 14. Applications Suitable for use in Western Blot, ELISA Recommended Dilution ELISA: 1:1,000 Western Blot: 1:100-500 Storage and Stability Store product at 4°C. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note Applications are based on unconjugated antibody. Immunogen PAPOLG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 684-714 amino acids from the C-terminal region of human PAPOLG. Formulation Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phophatase (AP). Purity Purified by Protein A affinity chromatography. Specificity Human Product Type Pab Source human Isotype IgG Grade Affinity Purified Applications E WB Crossreactivity Hu Storage 4°C Do Not Freeze Detection Method AP Reference Lee, Y.S., et al. J. Biol. Chem. 285(36):28105-28116(2010) Beausoleil, S.A., et al. Proc. Natl. Acad. Sci. U.S.A. 101(33):12130-12135(2004) Kyriakopoulou, C.B., et al. J. Biol. Chem. 276(36):33504-33511(2001) Topalian, S.L., et al. Mol. Cell. Biol. 21(16):5614-5623(2001) Perumal, K., et al. J. Biol. Chem. 276(24):21791-21796(2001) Powered by TCPDF (www.tcpdf.org).

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A Computational Approach for Defining a Signature of Β-Cell Golgi Stress in Diabetes Mellitus
Page 1 of 781 Diabetes A Computational Approach for Defining a Signature of β-Cell Golgi Stress in Diabetes Mellitus Robert N. Bone1,6,7, Olufunmilola Oyebamiji2, Sayali Talware2, Sharmila Selvaraj2, Preethi Krishnan3,6, Farooq Syed1,6,7, Huanmei Wu2, Carmella Evans-Molina 1,3,4,5,6,7,8* Departments of 1Pediatrics, 3Medicine, 4Anatomy, Cell Biology & Physiology, 5Biochemistry & Molecular Biology, the 6Center for Diabetes & Metabolic Diseases, and the 7Herman B. Wells Center for Pediatric Research, Indiana University School of Medicine, Indianapolis, IN 46202; 2Department of BioHealth Informatics, Indiana University-Purdue University Indianapolis, Indianapolis, IN, 46202; 8Roudebush VA Medical Center, Indianapolis, IN 46202. *Corresponding Author(s): Carmella Evans-Molina, MD, PhD ([email protected]) Indiana University School of Medicine, 635 Barnhill Drive, MS 2031A, Indianapolis, IN 46202, Telephone: (317) 274-4145, Fax (317) 274-4107 Running Title: Golgi Stress Response in Diabetes Word Count: 4358 Number of Figures: 6 Keywords: Golgi apparatus stress, Islets, β cell, Type 1 diabetes, Type 2 diabetes 1 Diabetes Publish Ahead of Print, published online August 20, 2020 Diabetes Page 2 of 781 ABSTRACT The Golgi apparatus (GA) is an important site of insulin processing and granule maturation, but whether GA organelle dysfunction and GA stress are present in the diabetic β-cell has not been tested. We utilized an informatics-based approach to develop a transcriptional signature of β-cell GA stress using existing RNA sequencing and microarray datasets generated using human islets from donors with diabetes and islets where type 1(T1D) and type 2 diabetes (T2D) had been modeled ex vivo. To narrow our results to GA-specific genes, we applied a filter set of 1,030 genes accepted as GA associated.
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Tommune Touttmann
TOMMUNEUS009952216B2TOUTTMANN (12 ) United States Patent ( 10 ) Patent No. : US 9 , 952 ,216 B2 Visel et al. (45 ) Date of Patent: Apr. 24 , 2018 ( 54 ) BRAIN - SPECIFIC ENHANCERS FOR C12N 15 / 86 (2006 .01 ) CELL -BASED THERAPY A61K 48 /00 (2006 .01 ) (52 ) U .S . CI. (71 ) Applicants :Axel Visel, El Cerrito , CA (US ) ; John CPC . .. .. GOIN 33 / 56966 (2013 .01 ) ; A61K 35 / 545 L . R . Rubenstein , San Francisco , CA ( 2013 .01 ) ; C12N 15 /85 ( 2013 .01 ) ; C12N (US ) ; Ying- Jiun ( Jasmine) Chen , 15 / 86 (2013 .01 ) ; GOIN 33/ 5014 ( 2013 .01 ) ; South San Francisco , CA ( US) ; Len A . A61K 48 / 00 ( 2013 .01 ) ; C12N 2830 /008 Pennacchio , Sebastopol, CA (US ) ; ( 2013 .01 ) Daniel Vogt, Burlingame, CA (US ) ; ( 58 ) Field of Classification Search Cory Nicholas, San Francisco , CA None (US ) ; Arnold Kriegstein , Mill Valley, See application file for complete search history . CA (US ) (56 ) References Cited ( 72 ) Inventors : Axel Visel , El Cerrito , CA ( US ) ; John L . R . Rubenstein , San Francisco , CA U . S . PATENT DOCUMENTS (US ) ; Ying - Jiun ( Jasmine ) Chen , 5 ,436 , 128 A * 7 /1995 Harpold . .. GOIN 33/ 6872 South San Francisco , CA (US ) ; Len A . 435 / 365 Pennacchio , Sebastopol, CA (US ) ; Daniel Vogt, Burlingame, CA (US ) ; OTHER PUBLICATIONS Cory Nicholas, San Francisco , CA (US ) ; Arnold Kriegstein , Mill Valley , Ferguson et al . The human synaptotagmin IV gene defines an CA (US ) evolutionary break point between syntenic mouse and human chro mosome regions but retains ligand inducibility and tissue specific ( 73 ) Assignee: The Regents of the University of ity . The Journal of Biological Chemistry , vol . 275 , No . 47 , pp . 36920 - 36926 , 2000 .
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