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Standards in Genomic Sciences (2012) 6:366-385 DOI:10.4056/sigs.2605792 Complete Genome Sequence of Paenibacillus strain Y4.12MC10, a Novel Paenibacillus lautus strain Isolated from Obsidian Hot Spring in Yellowstone National Park David A. Mead1,2, Susan Lucas3, Alex Copeland3, Alla Lapidus3, Jan-Feng Cheng3, David C. Bruce3,4, Lynne A. Goodwin3,4, Sam Pitluck2, Olga Chertkov3, Xiaojing Zhang3,4, John C. Detter3,4, Cliff S. Han3,4, Roxanne Tapia3,4, Miriam Land3,5, Loren J. Hauser3,5, Yun-juan Chang3, Nikos C. Kyrpides3, Natalia N. Ivanova3, Galina Ovchinnikova3, Tanja Woyke3, Catherine Brumm3, Rebecca Hochstein1, Thomas Schoenfeld1, and Phillip Brumm2* 1 Lucigen Corporation, Middleton, Wisconsin 2DOE Great Lakes Bioenergy Research Center, University of Wisconsin, Madison, Wisconsin 3DOE Joint Genome Institute, Walnut Creek, California 4Los Alamos National Laboratory, Bioscience Division, Los Alamos, New Mexico 5Oak Ridge National Laboratory, Oak Ridge, Tennessee *Corresponding author: Phillip Brumm ([email protected]) Keywords: Geobacillus sp. Y412MC10, Paenibacillus sp. Y412MC10, Obsidian Hot Spring Paenibacillus sp.Y412MC10 was one of a number of organisms isolated from Obsidian Hot Spring, Yellowstone National Park, Montana, USA under permit from the National Park Ser- vice. The isolate was initially classified as a Geobacillus sp. Y412MC10 based on its isolation conditions and similarity to other organisms isolated from hot springs at Yellowstone National Park. Comparison of 16 S rRNA sequences within the Bacillales indicated that Geobacillus sp.Y412MC10 clustered with Paenibacillus species, and the organism was most closely relat- ed to Paenibacillus lautus. Lucigen Corp. prepared genomic DNA and the genome was se- quenced, assembled, and annotated by the DOE Joint Genome Institute. The genome se- quence was deposited at the NCBI in October 2009 (NC_013406). The genome of Paenibacillus sp. Y412MC10 consists of one circular chromosome of 7,121,665 bp with an average G+C content of 51.2%. Comparison to other Paenibacillus species shows the organ- ism lacks nitrogen fixation, antibiotic production and social interaction genes reported in other paenibacilli. The Y412MC10 genome shows a high level of synteny and homology to the draft sequence of Paenibacillus sp. HGF5, an organism from the Human Microbiome Pro- ject (HMP) Reference Genomes. This, combined with genomic CAZyme analysis, suggests an intestinal, rather than environmental origin for Y412MC10. Abbreviations: YT, yeast extract tryptone broth, LB, Luria broth, TB, Terrific Broth Introduction Numerous novel microorganisms have been iso- Genome Institute, Lucigen Corp. isolated, charac- lated from hot springs in Yellowstone National terized, and sequenced a number of new isolates Park. Many of these organisms have been shown from Yellowstone hot springs. The bacterial iso- to possess enzymes with significant potential in late Y412MC10 was one of four microorganisms biotechnological applications [1]. Among the or- isolated from Obsidian Hot Spring, Yellowstone ganisms first isolated from Yellowstone hot National Park, Montana, USA and submitted for springs are Thermus aquaticus [2,3], Thermus whole genome sequencing. Y412MC10 was initial- brockianus [4], Acidothermus cellulollyticus [5], and ly classified as a Geobacillus sp. based on its isola- Synechococcus species [6]. As part of a project in tion conditions and morphological similarity to conjunction with the Department of Energy Joint other organisms such as Geobacillus species The Genomic Standards Consortium Mead et al. Y412MC61 (GenBank 544556), Y412MC52 most closely related to Paenibacillus lautus DSM (GenBank 550542), and Geobacillus 3055T (AB073188). The classification of the isolate thermoglucosidasius C56-YS93 (GenBank 634956). was confirmed using the EzTaxon-e server [24], The Geobacillus sp.Y412MC10 draft genome se- again on the basis of 16S rRNA sequence data. quence was deposited at the NCBI in October 2009 When compared to their entire r16S database, (NC_013406) with the lineage entry indicating Y412MC10 was identified as being a strain of that it is a Geobacillus. Following assembly of the Paenibacillus lautus with 99.09% identity and 100% complete genome of Y412MC10, the 16S rRNA se- completeness to the r16S of the type strain, quence and genome properties properly assigned Paenibacillus lautus NRRL NRS-666 GenBank the organism as a Paenibacillus sp. Y412MC10 D78472. represents the first Paenibacillus sp. isolated from a hot spring to have its genome completely se- Paenibacillus sp. Y412MC10 was one of a number quenced. of organisms isolated from Obsidian Hot Spring, Yellowstone National Park, Montana, USA Paenibacillus sp. were originally grouped in the (44.6100594° latitude and -110.4388217° longi- genus Bacillus until 1993, when Ash et al. [7] pro- tude) under a sampling permit from the National posed that members of "group 3" should be trans- Park Service. The hot spring possesses a pH of ferred to the genus Paenibacillus, and proposed 6.37 and a temperature range of 42-90°C. The or- Paenibacillus polymyxa as the type species. ganism was isolated from a sample of hot spring Paenibacillus sp. have been isolated from a wide water by enrichment and plating on YTP-2 medi- range of environments including soil [8], the Ant- um [25] (YTP-2 media contains (per liter) 2.0 g arctic [9], and the oral cavity of a dog [10]. yeast extract, 2.0 g tryptone, 2.0 g sodium py- Paenibacillus sp. are of interest for a number of ruvate, 1.0 g KCl, 2.0 g KNO3, 2.0 g Na2HPO4.7H2O, reasons, including production of antibiotics [11- 0.1 g MgSO4, 0.03 g CaCl2, and 2.0 ml clarified to- 13], biopolymer-degrading enzymes [14-16], and mato juice) at 50°C. Culture stocks were routinely their ability to fix nitrogen [17,18]. One species, P. maintained on YT (containing (per liter) 5.0 g vortex shows highly unusual organized growth yeast extract, 8.0 g tryptone, and 2.5 g NaCl) agar morphologies on solid surfaces [19,20]; another plates at 37°C. As part of the sequencing agree- species, P. dendritiformis, also shows unusual ment with the Joint Genome Institute, the culture growth morphologies on solid surfaces [19,21,22]. is available without restrictions from the authors. Comparison of the genetic content of Y412MC10 Lucigen, the National Park Service, and the Joint with genomes of Paenibacillus sp. from other envi- Genome Institute have placed no restrictions on ronments will give insights into the evolutionary the use of the culture or sequence data. adaptations that have occurred in the Y412MC10 is a Gram-positive facultative anaerobe Paenibacillus. The organism also may be a source (Table 1) that grows well on a wide variety of of novel polysaccharide degrading enzymes for standard lab media (YT, TB, LB). On plates, the use in biomass degradation. organism grows as rods or chains of rods (Figure 2A). After growth for 6 days on plates, the cells Classification and features still appear rod-shaped, but an extracellular ma- A phylogenetic tree was constructed to identify the trix appears to surround and bind the individual family relationship of strain Y412MC10 (Figure 1). cells together (light green background, Figure 2B). The tree was created using BLAST2Tree software In liquid culture, the organism appears to also [23]. The analysis was carried out using only type grow as a mixture of single cells and large clumps strains of validly-named organisms, and the analy- of cells surrounded by an extracellular matrix sis shows that Y412MC10 does not clade with (Figure 2C). Prolonged growth on plates or in liq- known Geobacillus species. Rather, Y412MC10 uid culture results in sporulation of the culture; clades in the Paenibacillus genus. Based on r16S spores are subterminal with swollen sporangia. analysis of validly-named organisms, Y412MC10 is http://standardsingenomics.org 367 Paenibacillus sp.Y412MC10 Figure 1. Phylogenetic tree highlighting the position of Paenibacillus lautus strain Y412MC10 and relative strains within the Bacillales. Strains used for the comparison (16S rRNA accession number) were (Paenibacillus Y412MC10); Paenibacillus lautus DSM 3035T (AB073188); Paenibacillus glucanolyticus DSM 5162T (AB073189); Paenibacillus illinoisensis DSM 11733T (AB073192); Paenibacillus chibensis DSM 11731T (AB073194); Paenibacillus ginsengisoli LMG 23406 T (AB245382); Paenibacillus macquariensis subsp defensor NCIB 14397 T (AB360546); Paenibacillus kribbensis KCTC 0766BP T (AF391123); Paenibacillus daejeonensis KCCM 41557 T (AF391124); Paenibacillus borealis DSM 13188 T (AJ011322); Paenibacillus odorifer DSM 15391 T (AJ223990); Paenibacillus cookie LMG 18419 T (AJ250317); Paenibacillus jamilae DSM 13815 T (AJ271157); Paenibacillus azoreducens DSM 13822 T (AJ272249); Paenibacillus stellifer DSM 14472 T (AJ316013); Paenibacillus peoriae DSM 8320 T (AJ320494); Paenibacillus cineris LMG 18349 T (AJ575658); Paenibacillus antarcticus LMG 22078 T (AJ605292); Paenibacillus favisporus LMG 20987 T (AY208751); Paenibacillus lactis DSM 15596 T (AY257868); Paenibacillus provencensis CIP 109358T (EF212893); Paenibacillus konsidanse KCTC 13165T (EU081509). 368 Standards in Genomic Sciences Mead et al. Figure 2A. Micrograph of Paenibacillus strain Y412MC10 cells showing individ- ual cells and chains of cells. Cells were streaked on YT agar and incubated 18 hr. at 37°C. A colony was removed, re-suspended in sterile water and stained using a 5 μM solution of SYTO® 9 fluorescent stain in sterile water (Molecular Probes). Dark field fluorescence
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  • Peptide-Based Quorum Sensing Systems in Paenibacillus Polymyxa

    Peptide-Based Quorum Sensing Systems in Paenibacillus Polymyxa

    Published Online: 6 August, 2020 | Supp Info: http://doi.org/10.26508/lsa.202000847 Downloaded from life-science-alliance.org on 1 October, 2021 Research Article Peptide-based quorum sensing systems in Paenibacillus polymyxa Maya Voichek1 , Sandra Maaß2 , Tobias Kroniger2,Dorte¨ Becher2, Rotem Sorek1 Paenibacillus polymyxa is an agriculturally important plant Many of the characteristic features associated with the above- growth–promoting rhizobacterium. Many Paenibacillus species mentioned behaviors—production and secretion of antimicrobials, are known to be engaged in complex bacteria–bacteria and expression of virulence factors, and forming complex colony bacteria–host interactions, which in other species were shown to structures—often require some form of intercellular communica- necessitate quorum sensing communication. However, to date, no tion such as quorum sensing (12). Bacteria use quorum sensing to quorum sensing systems have been described in Paenibacillus. coordinate gene expression patterns on a population level. For Here, we show that the type strain P. polymyxa ATCC 842 encodes example, the quorum sensing network of Pseudomonas aeruginosa at least 16 peptide-based communication systems. Each of these controls the production of secreted toxins as well as the production systems is comprised of a pro-peptide that is secreted to the of rhamnolipids, which are important for its biofilm architecture (13, growth medium and processed to generate a mature short peptide. 14). Quorum sensing regulates the production of bacteriocin an- Each peptide has a cognate intracellular receptor of the RRNPP timicrobials in Lactobacilli (15) and Streptococci (16, 17), and in P. polymyxa family, and we show that external addition of com- Bacillus subtilis, quorum sensing systems play a major role in munication peptides leads to reprogramming of the transcriptional sporulation, biofilm formation, and genetic competence (18).