SRBR 2006 Program Book
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Chronic Hyperaldosteronism in Cryptochrome-Null Mice Induces High-Salt- and Blood Pressure- Independent Kidney Damage in Mice
Hypertension Research (2014) 37, 202–209 & 2014 The Japanese Society of Hypertension All rights reserved 0916-9636/14 www.nature.com/hr ORIGINAL ARTICLE Chronic hyperaldosteronism in Cryptochrome-null mice induces high-salt- and blood pressure- independent kidney damage in mice Dwi Aris Agung Nugrahaningsih1, Noriaki Emoto1,2, Nicolas Vignon-Zellweger2, Eko Purnomo1, Keiko Yagi2, Kazuhiko Nakayama1,2, Masao Doi3, Hitoshi Okamura3 and Ken-ichi Hirata1 Although aldosterone has an essential role in controlling electrolyte and body fluid homeostasis, aldosterone also exerts certain pathological effects on the kidney. Several previous studies have attempted to examine these deleterious effects. However, the majority of these studies were performed using various injury models, including high-salt treatment and/or mineralocorticoid administration, by which the kidney changes observed were not only due to aldosterone but also due to prior injury caused by salt and hypertension. In the present study, we investigated aldosterone’s pathological effect on the kidney using a mouse model with a high level of endogenous aldosterone. We used cryptochrome-null (Cry 1, 2 DKO) mice characterized by high aldosterone levels and low plasma renin activity and observed that even under normal salt exposure conditions, these mice showed increased albumin excretion and kidney tubular injury, decreased nephrin expression and increased reactive oxygen species production in the absence of hypertension. Exposure to high salt levels exacerbated the kidney damage observed in these mice. Moreover, we noted that decreasing blood pressure without blocking aldosterone action did not provide beneficial effects to the kidney in high-salt-treated Cry 1, 2 DKO mice. Thus, our findings support the hypothesis that aldosterone has deleterious effects on the kidney independent of high-salt exposure and high blood pressure. -
SSHM Proceedings 1948-49
tcbe ~cotti~b ~ocietJ2 of tbe l)i~tor)2 of ~ebicine (Founded April, 1948.) REPORT OF PROCEEDINGS SESSION 1948.. 49. (!Cue .$cotttsb ,$ocictp of tbe JlistOiP of jRel:1icine. President Dr. DOUGLAS GUTHRIE. V iee-P l'esiden ts Mr W. 1. STUART. Profe3sor G. B. FLEMING (Glasgow) Hon. Secretary Dr. H. P. TAIT. Hon. TreaSUI'er Dr. W. A. ALEXANDER. Council Sir HENRY WADE. Dr. W. D. D. SMALL. Brig.-Gen. SUTTON. Professor CAMPBELL (Aberdeen). Dr. JOHN RITCHIE. Dr. HENRY GIBSON (Dundee). Dr. WILKIE MILLAR. Professor CHAS. M'NEIL. Mr A. L. GOODALL (Glasgow). The Senior President, Royal Medical Society. E ~bt 8tottisb ~otietp of tbe j!)istorp of ~ebicine. For many years it had been felt that there was a need for a Society in Scotland primarily devoted to the study of the History of Medicine and its allied Sciences. Such a Society came into being on 23rd April 1948, when a well attended and representative gathering of medical men and other interested persons from all over Scotland met in the Hall of the Royal College of Surgeons of Edinburgh. It was then agreed to constitute the Society and to call it "The Scottish Society of the History of Medicine." A Constitution was drawn up and Office-Bearers for the ensuing year were elected. From this beginning the Society has grown steadily and now has a membership of some hundred persons. After the business of this Preliminary 1\1 eeting had been carried through, the Medical Superintendent of the Royal Infirmary of Dundee, Dr. Henry J. C. -
Faculty Mentor List Basic Science Faculty Mentors 1. Joseph
Faculty Mentor List Basic Science faculty mentors 1. Joseph Takahashi, Ph.D. Professor and Chair, Department of Neuroscience [email protected] Research Statement: The long-term goals of the Takahashi laboratory are to understand the molecular and genetic basis of circadian rhythms in mammals and to utilize forward genetic approaches in the mouse as a tool for gene discovery for complex behavior. We have focused our attention in three areas: 1) identification of circadian clock genes and assignment of their function in the molecular mechanism of the circadian pacemaker; 2) analysis of central and peripheral circadian oscillators using real-time circadian reporters; and 3) identification of genes defined by mutations isolated in the large-scale mutagenesis screens we have conducted on neural and behavioral phenotypes including psychostimulant responses and contextual and cue dependent fear conditioning. Recently, we have focused on the structural biology of circadian clock proteins and on genomewide analysis of transcription factor binding and gene expression using next generation sequencing methods. A comprehensive global analysis of circadian transcription factor binding in the mouse liver has been completed in which all core components of the clock gene pathway have been interrogated. In addition, we have also analyzed the genome-wide regulation of nascent transcription, RNA polymerase II occupancy and epigenomic regulation of chromatin by the circadian clock. We have recently identified genes involved in cocaine responsiveness using forward genetic approaches. My laboratory has demonstrated a successful record of research productivity and training in circadian biology, mammalian genetics, genomics and molecular biology. 1. Huang, N, Y Chelliah, Y Shan, CA Taylor, S-H Yoo, C Partch, CB Green, H Zhang, JS Takahashi 2012 Crystal structure of the heterodimeric CLOCK:BMAL1 transcriptional activator complex. -
Paul Hardin, Ph.D. John W
Department of Biology The College of Arts + Sciences | Indiana University Bloomington About Paul Hardin Distinguished Alumni Award Lecture Thu., Oct. 18, 2018 • 4 to 5 pm • Myers Hall 130 Paul Hardin, Ph.D. John W. Lyons Jr. ’59 Chair in Biology, Texas A&M University Genetic architecture underlying circadian clock initiation, maintenance, and output in Drosophila Circadian clocks drive daily rhythms in metabolism, physiology, and behavior in organisms ranging from cyanobacteria to humans. The identification and analysis of “clock genes” in Drosophila revealed that circadian timekeeping is based on a transcriptional feedback loop Paul Hardin studied the development of the sea in which CLOCK-CYCLE (CLK-CYC) heterodimers activate transcription of their feedback urchin embryo in William Klein’s lab at Indiana repressors PERIOD (PER) and TIMELESS (TIM). Subsequent studies revealed that similar University, from where he received his Ph.D. in transcriptional feedback loops keep circadian time in all eukaryotes and, in the case of 1987. He did his postdoctoral fellowship with animals, that these feedback loops are comprised of conserved components. The “core” Michael Rosbash at Brandeis University, working feedback loop described above operates in conjunction with an “interlocked” feedback on the circadian rhythms of the fruit fly, Drosophila loop in animals to drive rhythmic transcription of hundreds of genes that are maximally melanogaster. His work with Michael Rosbash and expressed at different phases of the circadian cycle. These feedback loops operate in many, Jeff Hall has been instrumental to our understanding but not all, tissues in flies including the brain pacemaker neurons that control rest:activity of how circadian rhythms affect a myriad of rhythms. -
CURRICULUM VITAE Joseph S. Takahashi Howard Hughes Medical
CURRICULUM VITAE Joseph S. Takahashi Howard Hughes Medical Institute Department of Neuroscience University of Texas Southwestern Medical Center 5323 Harry Hines Blvd., NA4.118 Dallas, Texas 75390-9111 (214) 648-1876, FAX (214) 648-1801 Email: [email protected] DATE OF BIRTH: December 16, 1951 NATIONALITY: U.S. Citizen by birth EDUCATION: 1981-1983 Pharmacology Research Associate Training Program, National Institute of General Medical Sciences, Laboratory of Clinical Sciences and Laboratory of Cell Biology, National Institutes of Health, Bethesda, MD 1979-1981 Ph.D., Institute of Neuroscience, Department of Biology, University of Oregon, Eugene, Oregon, Dr. Michael Menaker, Advisor. Summer 1977 Hopkins Marine Station, Stanford University, Pacific Grove, California 1975-1979 Department of Zoology, University of Texas, Austin, Texas 1970-1974 B.A. in Biology, Swarthmore College, Swarthmore, Pennsylvania PROFESSIONAL EXPERIENCE: 2013-present Principal Investigator, Satellite, International Institute for Integrative Sleep Medicine, World Premier International Research Center Initiative, University of Tsukuba, Japan 2009-present Professor and Chair, Department of Neuroscience, UT Southwestern Medical Center 2009-present Loyd B. Sands Distinguished Chair in Neuroscience, UT Southwestern 2009-present Investigator, Howard Hughes Medical Institute, UT Southwestern 2009-present Professor Emeritus of Neurobiology and Physiology, and Walter and Mary Elizabeth Glass Professor Emeritus in the Life Sciences, Northwestern University -
Department of Systems Biology
DIVISION OF BIOINFORMATICS AND CHEMICAL GENOMICS Research Profile Department of Systems Biology Professor: Hitoshi Okamura, Associate Professor: Masao Doi, Assistant Professor: Yoshiaki Yamaguchi, Senior Lecturer: Jean-Michel Fustin Research Projects: How TIME is generated and tuned? We will clarify feedback loop of clock genes. the secret of generation and tuning of TIME in 1.3 Clock genes and cell metabolism, birth, and mammalian circadian system by multi-layered view death at intracellular, intercellular and individual levels. Why virtually all cells in the body have the clock Through clarifying the integration network mecha- inside the cell? We will identify how clock genes nism of TIME, we will develop new drugs for tuning work on the energy metabolism, cell cycles, and TIME. cell death. The subject of our study is circadian timing system 2. Intercellular system for synchronizing TIME in mammals. In this system, the circadian TIME gen- 2.1 Region-specific knockdown of SCN erated at molecular clock in the suprachiasmatic SCN biological clock is composed of thousands nucleus (SCN) evokes the synchronized oscillation of clock cells which are subdivided into several of molecular clocks in the whole body. Between groups. We will perform region-specific knockdown them, TIME is transmitted in multilayer systems: 1) of these subdivisions to address the functional sub- intracellular system of generation of cyclic TIME, 2) division of SCN. Intercellular system for synchronizing TIME, and 3) 2.3 Geography of SCN Symphony of TIME in individuals. SCN clock cells are highly organized in time and 1. Clarification of clock machinery to generate space. For example, in our real-time luciferase- TIME imaging system at cell level, time is generated and 1.1 Identification of all components of CLOCK synchronized in a very highly organized system. -
Antibodies Against the Clock Proteins Period and Cryptochrome Reveal the Neuronal Organization of the Circadian Clock in the Pea Aphid
ORIGINAL RESEARCH published: 02 July 2021 doi: 10.3389/fphys.2021.705048 Antibodies Against the Clock Proteins Period and Cryptochrome Reveal the Neuronal Organization of the Circadian Clock in the Pea Aphid Francesca Sara Colizzi 1, Katharina Beer 1, Paolo Cuti 2, Peter Deppisch 1, David Martínez Torres 2, Taishi Yoshii 3 and Charlotte Helfrich-Förster 1* 1Neurobiology and Genetics, Theodor-Boveri-Institute, Biocenter, University of Würzburg, Würzburg, Germany, 2Institute for Integrative Systems Biology (I2SysBio), University of Valencia and CSIC, Valencia, Spain, 3Graduate School of Natural Science and Technology, Okayama University, Okayama, Japan Circadian clocks prepare the organism to cyclic environmental changes in light, temperature, Edited by: or food availability. Here, we characterized the master clock in the brain of a strongly Joanna C. Chiu, photoperiodic insect, the aphid Acyrthosiphon pisum, immunohistochemically with antibodies University of California, Davis, United States against A. pisum Period (PER), Drosophila melanogaster Cryptochrome (CRY1), and crab Reviewed by: Pigment-Dispersing Hormone (PDH). The latter antibody detects all so far known PDHs and Hideharu Numata, PDFs (Pigment-Dispersing Factors), which play a dominant role in the circadian system of Kyoto University, Japan many arthropods. We found that, under long days, PER and CRY are expressed in a rhythmic Annika Fitzpatrick Barber, Rutgers, The State University of manner in three regions of the brain: the dorsal and lateral protocerebrum and the lamina. No New Jersey, United States staining was detected with anti-PDH, suggesting that aphids lack PDF. All the CRY1-positive *Correspondence: cells co-expressed PER and showed daily PER/CRY1 oscillations of high amplitude, while Charlotte Helfrich-Förster charlotte.foerster@biozentrum. -
The Circadian Clock Modulates Core Steps in Long-Term Memory Formation in Aplysia
8662 • The Journal of Neuroscience, August 23, 2006 • 26(34):8662–8671 Cellular/Molecular The Circadian Clock Modulates Core Steps in Long-Term Memory Formation in Aplysia Lisa C. Lyons, Maria Sol Collado, Omar Khabour, Charity L. Green, and Arnold Eskin Department of Biology and Biochemistry, University of Houston, Houston, Texas 77204-5001 The circadian clock modulates the induction of long-term sensitization (LTS) in Aplysia such that long-term memory formation is significantly suppressed when animals are trained at night. We investigated whether the circadian clock modulated core molecular processes necessary for memory formation in vivo by analyzing circadian regulation of basal and LTS-induced levels of phosphorylated mitogen-activated protein kinase (P-MAPK) and Aplysia CCAAT/enhancer binding protein (ApC/EBP). No basal circadian regulation occurred for P-MAPK or total MAPK in pleural ganglia. In contrast, the circadian clock regulated basal levels of ApC/EBP protein with peak levels at night, antiphase to the rhythm in LTS. Importantly, LTS training during the (subjective) day produced greater increases in P-MAPK and ApC/EBP than training at night. Thus, circadian modulation of LTS occurs, at least in part, by suppressing changes in key proteins at night. Rescue of long-term memory formation at night required both facilitation of MAPK and transcription in conjunction with LTS training, confirming that the circadian clock at night actively suppresses MAPK activation and transcription involved in memory formation. The circadian clock appears to modulate LTS at multiple levels. 5-HT levels are increased more when animals receive LTS training during the (subjective) day compared with the night, suggesting circadian modulation of 5-HT release. -
Wednesday 12Th December 2018 Retiral of Norma Watson from FDCA Committee Purchase of Photograph of Dundee Polic
Friends of Dundee City Archives newsletter Winter 2018 17 Cake and Chat: Wednesday The Great War: Dundee & The 12th December 2018 Home Front We have arranged a Christmas Cake and Chat to be held in Committee Room 1 between 2pm and 4 pm. This room is accessed from 14 City Square and is in the corridor leading to the Archives office. We look forward to seeing you. Linda Nicoll’s book was launched at the Wighton Centre on 8 November. The book, Retiral of Norma Watson which is only available from FDCA Committee from the City Archives, is selling It was with regret that the members of the well. If you or any of FDCA Committee received Norma’s decision your family or friends to retire. Norma served as Hon. Treasurer would like a copy, from 2011 until 2016 and remained on the please either call at the Committee for a further two years. archives during office hours or apply by post, The Volunteers miss her cheery banter on email ([email protected]) or Wednesday mornings. We all wish her well in telephone (01382 434494) to Dundee City her many interests. Archives. The book costs £9.99 plus post and Purchase of Photograph of packing. Dundee Police Pipe Band This Poppy has all the names on the Dundee FDCA purchased a black and white Roll of Honour on its petals. It was part of a photograph of Dundee Police Pipe Band in the display at Fintry Primary School in 1930s for the archives. The photograph was commemoration of the 100th anniversary of taken on the steps leading to what was then the the Armistice. -
Photoperiodic Responses on Expression of Clock Genes, Synaptic Plasticity Markers, and Protein Translation Initiators the Impact of Blue-Enriched Light
Photoperiodic Responses on Expression of Clock Genes, Synaptic Plasticity Markers, and Protein Translation Initiators The Impact Of Blue-Enriched Light Master report Jorrit Waslander, s2401878 Behavioral Cognitive Neuroscience research master, N-track University of Groningen, the Netherlands Internship at: Bergen Stress and Sleep Group, University of Bergen, Norway Date: 13-7-2018 Internal supervisor, University of Groningen: P. (Peter) Meerlo External supervisor, University of Bergen: J. (Janne) Grønli Daily supervisor, University of Bergen: A. (Andrea) R. Marti Photoperiodic Responses in the PFC Table of Contents Summary ................................................................................................................................................. 3 Introduction ............................................................................................................................................. 5 Research Objective .............................................................................................................................. 8 Hypotheses .......................................................................................................................................... 8 Methods ................................................................................................................................................ 10 Experimental procedure .................................................................................................................... 10 Ethics ............................................................................................................................................ -
Single-Cell Analysis on the Biological Clock Using Microfluidic
SINGLE-CELL ANALYSIS ON THE BIOLOGICAL CLOCK USING MICROFLUIDIC DROPLETS by ZHAOJIE DENG (Under the Direction of Leidong Mao and Jonathan Arnold) ABSTRACT Single-cell analysis has become crucial for uncovering the underlying mechanism for cell heterogeneity. Different biological questions pose different challenges for single-cell analysis. In order to answer questions like, whether a single-cell has a biological clock and how the clocks synchronize among cells to overcome the heterogeneity, continuous long-term measurement on large numbers of single-cells is required. However traditional measurement techniques usually involve measurement on millions of cells. My dissertation addresses these challenges by developing a microfluidic droplet platform capable of measuring the biological clock on >1000 Neurospora crassa single-cells for up to 10 days. The results show that in Neurospora crassa a single cell has the three major properties of a biological clock: a circadian oscillator, light entertainment, and temperature compensation and that single-cells synchronize their biological clock with each other possibly through quorum sensing. INDEX WORDS: Single-cell analysis, Microfluidic droplets, Neurospora crassa, Circadian rhythm, Biological clock, Stochastic noise, Synchronization SINGLE-CELL ANALYSIS ON THE BIOLOGICAL CLOCK USING MICROFLUIDIC DROPLETS by ZHAOJIE DENG B.A., Huazhong University of Science and Technology, China, 2008 M.S., Huazhong University of Science and Technology, China, 2011 A Dissertation Submitted to the Graduate Faculty -
Challenging the Human Circadian Clock by Daylight Saving Time and Shift-Work
Challenging the human circadian clock by Daylight Saving Time and Shift-Work Academic Dissertation (Doctor rerum naturalium) At the Centre for Chronobiology Institute for Medical Psychology Ludwig-Maximilians-University Munich Written by Thomas Kantermann Born 26th of February, 1979 in Gütersloh Arbeit eingereicht am: 17.07.2008 1. Gutachter / Prüfer: Prof. Gisela Grupe 2. Gutachter / Prüfer: Prof. Benedikt Grothe 3. Prüfer: Prof. Susanne Foitzik 4. Prüfer: Prof. Gerhard Haszprunar Sondergutachter: Prof. Till Roenneberg Tag der mündlichen Prüfung: 15.12.2008 Für meine Schwester Stefanie „Probleme kann man niemals mit derselben Denkweise lösen, durch die sie entstanden sind.“ Albert Einstein dt.-amerikan. Physiker, 1921 Nobelpreis für Physik 1879 – 1955 Contents 1. INTRODUCTION ..................................................................................... 1 1.1. Biological (circa-) Rhythms..........................................................................................2 1.2. Sleep.............................................................................................................................4 1.2.1. Two Process Model of Sleep..................................................................................6 1.2.2. Circadian Rhythm Sleep Disorders, Sleepiness and Fatigue....................................7 1.3. The Internal Clock ........................................................................................................8 1.3.1. Phase of Entrainment – Chronotype .....................................................................11