Control of FMD and other exotic diseases in

Damyan Iliev, Georgi Chobanov & Tsviatko Alexandrov

FAO-EuFMD/EC/OIE Tripartite Group Meeting on control of FMD and other exotic diseases in the Southern

Alexandropoulos, 31.08 – 01.09.2015

31/08 – 01/09 2015

Lumpy skin disease

LSD outbreaks in 2013 – Aug 2015

31/08 – 01/09 2015 • Feb 2015: Mission to Adana and Hatay for 3 experts from BG to learn more about LSD; • Acknowledgements to the Turkish colleagues, EuFMD, Eeva Tuppurainene.

31/08 – 01/09 2015 Trainings and awareness campaigns

• Trainings to all official vets; • cascade training to private vets and farmers; • Access of all stakeholders to all materials and presentations • Instruction for farmers for disinsection; • Participation of Bulgarian vets to the workshop on LSD in Alexandropoulos (acknowledgments to the Drs. Spiros Doudounakis and Dimitrios Dilaveris and all the other Greeck colleagues).

31/08 – 01/09 2015 Bulgaria flooded with leaflets for LSD

31/08 – 01/09 2015 Measures under Order РД 11-1054/22.06.2015  Enforced epidemiological preparedness in the regions of , , and Kardjali;  Weekly clinical examinations in the municipalities bordering and ;  Ban for animal markets in border municipalities;  Enforced controls on the identification of all cattle and update in National electron database;  Regional and municipal epizootic commissions;  Information for mares, farmers and all stakeholders about clinical signs, biosecurity measures and their obligations in case of suspicions;  Enforced controls on animal movements and biosecurity measures;  Weekly reports to be provided to BFSA HQ .

31/08 – 01/09 2015 Measures under Order РД 11-1388/20.08.2015 • and regions included in the enforced control under Order РД 11-1054/22.06.2015; • Ban on movements of large and small ruminants in the regions of Burgs, Yambol, Haskovo, Kardjali, Smolyan and Blagoevgrad; • Reports about the clinical examinations, identification and all other controls to be provided to BFSA HQ on daily basis.

31/08 – 01/09 2015 Clinical surveillance for LSD • Till end of June 2015 clinical examinations of all cattle in the villages bordering Turkey once a month under the programe; • 88.6% of the cattle population checked during the vaccination campaign against BTV 4 in spring – summer of 2015 • Since 22.06 clinical examinations for LSD on weekly basis in the south border areas:

Number of herds Number of animals clinically Municipality examined examined 61 1821 M. Tarnovo 36 856 114 7287 85 2416 204 3604 54 2638 41 2406 All 595 21028 31/08 – 01/09 2015 Three suspicions for LSD reported, all with negative results

31/08 – 01/09 2015 31/08 – 01/09 2015 PPR, SGP and other exotic diseases

31/08 – 01/09 2015 4 1 2 SGP outbreaks in 2013 – May 2015 3

Surveillance for SGP and PPR in 2014 and 2015 is in the framework of the THRACE programme

31/08 – 01/09 2015 Surveillance for SGP and PPR in 2015 (by mid Aug)

Number of sheep and 88.64% of the sheep Municipality goats clinically population checked examined Tsarevo 3291 during the M. Tarnovo 1561 vaccination campaign Sredets 610 against BTV 4 in Bolyarovo 610 spring – summer of Elhovo 3291 2015 Topolovgrad 1319 Svilengrad 1852 All 12543

31/08 – 01/09 2015 Suspicion for SGP, Ravadinovo, Burgas region with negative result

31/08 – 01/09 2015 Posters and leaflets for SGP and PPR

31/08 – 01/09 2015 Bluetongue

31/08 – 01/09 2015

BT outbreaks31/08 – 01/09 2015 in 2014 – May 2015 First outbreak confirmed on 04 July 2014 on the southeastern border In 2014 BTV-4 confirmed in all 28 administrative districts

31/08No – 01/09 new 2015 outbreaks in 2015 Data on infected herds and animals for the period 04 Jul – 31 Dec 2014

Bovines Goats Sheep Herds, n 83 747 49 816 86 266 Animals, n 632 553 383 755 1 558 222 Infected herds, n 808 391 6 061 Animals in the 26 036 / 4.17 % 12 173 / 3.17% 533 733 / 34.25% infected herds, n Fallen stock/ 410 / 1.5% 258 / 2.11% 27 040 / 5.06% animals culled

Measures enforced: In accordance with Regulation1266/2007 Movement restrictions Enforced surveillance Disinsection of animals, holdings, pastures, water reservoirs Compensations to owners for culled/dead animals 31/08 – 01/09 2015 Vaccination against BTV 4 in 2015

Blanket vaccination for sheep and bovines with monovalent inactivated serotype 4 vaccine BTVPUR AlSap 4, Merial in accordance with the 2015 BT programme approved by EC;

Number Number Number of Number of Number of Number of of of vaccinated re- ovines vaccinated villages bovines bovines / % vaccinated ovines /% bovines / % 545 575/ 327 909/ 1 347 420/ Total 5216 615 791 1 520 036 88,6 % 60,1 % 88,64 %

31/08 – 01/09 2015 Surveillance for BTV in Culicoides Methods: • BTV NS3 PCR (serotype 1-26) including an Internal positive control (Teramo protocol) • Serotype specific PCR (LSI) BTV NS3 positive samples are further tested with serotype specific PCR. Samples with inhibition are diluted and retested with the same protocol. Results: Sample Province Location Date Species Number Age grading BTV PCR Serotype ID of insects (serotype 1-26) specific BTV PCR 1 Kyosevo 4/16/2015 C. punctatus 8 parous inhibition* - 2 Kardzhali Kyosevo 4/16/2015 C. pulicaris 6 parous inhibition* - 3 Kardzhali Kyosevo 4/16/2015 Obsoletus complex 7 engourged Negative - 4 Kardzhali Kyosevo 4/16/2015 Obsoletus complex 2 parous inhibition* - 5 Blagoevgrad 4/16/2015 C. punctatus 2 engourged Negative - 6 Blagoevgrad Hadzhidimovo 4/16/2015 C. punctatus 3 parous inhibition* - 7 Blagoevgrad Hadzhidimovo 4/16/2015 C. pulicaris 8 parous inhibition* - 8 Blagoevgrad Hadzhidimovo 4/16/2015 Obsoletus complex 4 engourged inhibition* - 9 Blagoevgrad Hadzhidimovo 4/16/2015 Obsoletus complex 8 parous Negative - 10 Blagoevgrad Hadzhidimovo 4/16/2015 C. newsteadi 8 parous Negative - 11 Kardzhali Kobilyane 5/14/2015 C. pulicaris 21 engourged Negative - 12 Kardzhali Kobilyane 5/14/2015 C. pulicaris 50 parous Negative - 13 Kardzhali Kobilyane 5/14/2015 C. pulicaris 32 parous Negative - 14 Kardzhali Kobilyane 5/14/2015 C. punctatus 9 engourged Negative - 15 Kardzhali Kobilyane 5/14/2015 C. punctatus 19 parous Negative - 16 Kardzhali Kobilyane 5/14/2015 Obsoletus complex 23 engourged Negative - 17 Kardzhali Kobilyane 5/14/2015 Obsoletus complex 50 parous Negative - 18 Kardzhali Kobilyane 5/14/2015 Obsoletus complex 5 parous Negative - 31/08 – 01/09 2015 * lab investigation continuing

HPAI H5N1 Black Burgas sea 3 2 1

Location date Species affected 1 Poda, Burgas 22.01.2015 Dalmatian Pelican 2 Konstantinovo, Burgas 30.01.2015 Domestic poultry 3 of Burgas 03.02.2015 Rock dove, Black headed gull Srebarna, Silsitra 24.03.2015 Dalmatian pelicans 31/08 – 01/09 2015 HPAI H5N1 in Burgas region 22 Jan – 03 Feb 2015

Burgas, 3 Feb • Rock dove • Black headed gull

Konstantinovo, 30 Jan Backyard poultry Poda, 22 Jan 2015 Dalmatian Pelican

31/08 – 01/09 2015 Measures taken All measures pursuant Council Directive 2005/94/EC on Community measures for the control of avian influenza, Commission Decisions 2006/563/EC; 2006/415/EC and 2006/416/EC and the National contingency plan for AI were immediately put in force; Movement of birds and products thereof was immediately imposed in the protection and surveillance zones. Clinical examinations in the zones are to be performed on daily basis; Stamping out of all birds in Konstantinovo; Cleaning and disinfection; The surveillance for AI, as well as bio-security measures in all kind of bird farms and highly concentrated with wild birds water pools are enforced all over Bulgaria; Ban of markets and exhibitions for birds was imposed on the whole territory of the country; Hunting for birds was banned on the territory of the high risk areas for AI, namely Burgas, Varna, , Silistra, , , Yambol, , , Ruse and regions; Fishing banned in the highly concentrated with wild birds water pools.

31/08 – 01/09 2015 Areas A and B established in accordance with Commission implementing Decision (EU) 2015/205 up to 5 March 2015

31/08 – 01/09 2015 HPAI H5N1 in Silsitra region

24 Mar – signal for 21 Dalmatian Pelicans found dead in Srebarna Natural Reserve 2 pelicans sampled 26 Mar - H5N1 confirmed Measures provided for in Commission Decision 2006/563/EC

31/08 – 01/09 2015 Romania Protection and Danube surveillance zones

Bulgaria

zone village Backyards, n Poultry, n Waterfowls, n All birds, n 3km zone Srebarna 200 3520 33 3553 Vetren 67 1431 16 1447 Aidemir 202 2944 35 2979 10 km Lambrinovo 27 362 7 369 surveillance zone Sitovo 134 1720 60 1780 Popina 63 903 17 920 Polyana 45 314 18 332 Total 7 738 11194 186 11380 31/08 – 01/09 2015 Measures in place 3 km protection zone and 10 km surveillance zone immediately established. Movement of birds and products thereof was immediately imposed in the 3 km protection and the 10 km surveillance zones. Clinical examinations in the zones are to be performed on daily basis. The surveillance for AI, as well as bio-security measures in all kind of bird farms and highly concentrated with wild birds water pools are enforced all over Bulgaria. Ban of markets and exhibitions for birds was imposed on the whole the territories of Silistra, Ruse, Shumen, Razgrad, Dobrich and Varna regions. Up to now no morbidity and/or mortality rates have been detected in domestic and wild birds in the zones and in any other settlements on the territory of Silistra region and the whole country.

31/08 – 01/09 2015 Oral vaccination of foxes against rabies in 2015

31/08 – 01/09 2015 Foot-and-mouth disease

FMD outbreaks, 2010 - May 2015

31/08 – 01/09 2015 FMD surveillance in domestic livestock in 2014

 Sero-surveillance in sheep and goats and  clinical examinations of all susceptible livestock on monthly basis

All Settlements, Municipality samples n for 2014

Tsarevo 4 723 M. Tarnovo 3 480 Sredets 3 720 Bolyarovo 2 480 Elhovo 3 720 Topolovgrad 3 701 Svilengrad 3 720 All 21 4544 31/08 – 01/09 2015 Surveillance for FMD in wild boar, Hunting season 2014 – 2015

3711 wild boar shot all over Bulgaria examined. No lesions suggested FMD were detected

Number of wild boar tested serologically and virologically for FMD Region Burgas Yambol Haskovo Total Municipality Tsarevo Sredets Boliarovo Elhovo Topolovgrad Svilengrad Wild boar tested, n 108 23 69 79 120 5 11 415

31/08 – 01/09 2015 FMD surveillance in domestic livestock in 2015  Sero-surveillance in sheep and goats and  clinical examinations of all susceptible livestock on monthly basis till end of June; Since July – twice a month;

All Settlements, Municipality samples by n Aug 2015

Tsarevo 4 480 M. Tarnovo 3 320 Sredets 3 480 Bolyarovo 2 360 Elhovo 3 540 88.6% of the cattle population and 88.64% Topolovgrad 3 420 of the sheep checked during the vaccination Svilengrad 3 420 campaign against BTV 4 in spring – summer of 2015 All 21 3020 31/08 – 01/09 2015 Inspections at abattoirs

cattle small ruminants pigs 2014 22565 239620 877891 2015 (by Jul) 11720 147442 853954 all 34285 387062 1331845

31/08 – 01/09 2015 Emergency preparedness trainings for FMD Balkan workshops • Outbreak management, field investigation and contingency planning – Dec 2013, Skopje • Crisis management and crisis communication – February 2014, • FMD contingency planning for NRL – April 2014, Kraljevo • Surveillance – November 2014, Sofia Lab trainings • FMD laboratory training • Biosafety officer training

Simulation exercises • SimEx 1 – January 2015, Desktop exercise carried out simultaneously in Serbia, FYROM and Bulgaria • SimEx 2 – June 2015, Desktop and basement exercise, filed visit 31/08 – 01/09 2015 Thank you!

31/08 – 01/09 2015