bioRxiv preprint doi: https://doi.org/10.1101/175380; this version posted August 11, 2017. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY 4.0 International license. 1 Increased anxiety and decreased sociability in adulthood following 2 paternal deprivation involve oxytocin in the mPFC 3 4 Zhixiong He a, Limin Wang a, Luo luo a, Rui Jia ab, Wei Yuan a, Wenjuan Hou a, Jinfeng Yang a, Yang 5 Yang a, Fadao Tai* ab 6 7 a Institute of Brain and Behavioral Sciences, College of Life Sciences, Shaanxi Normal University, Xi’an, 710062, China 8 b Cognition Neuroscience and Learning Division, Key Laboratory of Modern Teaching Technology, Ministry of Education, Shaanxi 9 Normal University, Xi’an, 710062, China 10 11 12 Correspondence to Fadao Tai, Institute of Brain and Behavioral Sciences, College of Life Sciences, 13 Shaanxi Normal University, Xi’an, Shaanxi 710062, China. fax: +86-29-85308436. E-mail: 14
[email protected] 15 16 Abstract Early adverse experiences often have devastating consequences on adult emotional and 17 social behavior. However, whether paternal deprivation (PD) during the pre-weaning period 18 affects brain and behavioral development remains unexplored in socially mandarin vole (Microtus 19 mandarinus). We found that PD increased anxiety-like behavior and attenuated social preference 20 in adult males and females; decreased prelimbic cortex OT-immunoreactive fibers and 21 paraventricular nucleus OT positive neurons; reduced levels of medial prefrontal cortex (mPFC) 22 OT receptor protein in females and OT receptor and V1a receptor protein in males.