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Rapport D'activités Type Rapport annuel d’activité 2018 Centre de national de Année d’exercice référence Mycoses Invasives et Antifongiques 2017 Responsables Pr. Françoise Dromer Pr. Stéphane Bretagne, adjoint Pr. Olivier Lortholary, adjoint Collaborateurs M. Alexandre Alanio, médecin mycologue Mme Anne Boullié, technicienne Mme Reine Bouyssié, assistante Mme Marie Desnos-Ollivier, ingénieur Mme Dea Garcia-Hermoso, ingénieur Mme Cécile Gautier, technicienne Mme Fanny Lanternier, médecin clinicien Mme Karine Sitbon, médecin d'études cliniques RESUME ANALYTIQUE ....................................................................................................................... 1 SUMMARY ........................................................................................................................................ 2 1 MISSIONS ET ORGANISATION DU CNR ....................................................................................... 3 2 ACTIVITES D’EXPERTISE ............................................................................................................. 3 2.1 ÉVOLUTIONS DES TECHNIQUES ........................................................................................................... 3 2.2 TRAVAUX D’EVALUATION DES TECHNIQUES, REACTIFS ET TROUSSES .......................................................... 4 2.3 TECHNIQUES TRANSFEREES VERS D'AUTRES LABORATOIRES ..................................................................... 4 2.4 COLLECTIONS DE MATERIEL BIOLOGIQUE .............................................................................................. 4 2.5 ACTIVITES D’EXPERTISE ..................................................................................................................... 5 2.5.1 Expertise 2017 sur les levures ................................................................................................. 6 2.5.2 Expertise 2017 sur les champignons filamenteux ................................................................... 9 2.5.3 Expertise 2017 sur la sensibilité aux antifongiques .............................................................. 13 2.5.4 Expertise 2017 sur les mycoses endémiques ........................................................................ 14 2.5.5 Expertise 2017 en anatomopathologie ................................................................................. 15 2.5.6 Expertise 2017 pour les diagnostics moléculaires ................................................................ 15 2.6 ACTIVITES DE SEQUENÇAGE ............................................................................................................. 16 3 ACTIVITES DE SURVEILLANCE ................................................................................................... 16 3.1 DESCRIPTION DU RESEAU DE PARTENAIRES ......................................................................................... 17 3.2 SURVEILLANCE DE L’EVOLUTION ET DES CARACTERISTIQUES DES INFECTIONS ............................................ 19 3.2.1 Cryptococcoses ..................................................................................................................... 20 3.2.2 Fongémies ............................................................................................................................. 21 3.2.3 Pneumocystoses ................................................................................................................... 22 3.2.4 Mucormycoses ...................................................................................................................... 23 3.2.5 Aspergilloses ......................................................................................................................... 24 3.2.6 Fusarioses ............................................................................................................................. 26 3.3 SURVEILLANCE DE LA RESISTANCE DES AGENTS PATHOGENES AUX ANTI-INFECTIEUX ................................... 26 3.3.1 Définitions utilisées pour exprimer la résistance .................................................................. 27 3.3.2 Résultats de la surveillance des résistances aux antifongiques pour les levures .................. 28 3.3.3 Résultats de la surveillance des résistances aux antifongiques pour les filamenteux .......... 30 3.3.4 Analyse des tendances .......................................................................................................... 33 3.4 INTERFACES AVEC LES RESEAUX DE SURVEILLANCE NATIONAUX OU INTERNATIONAUX................................. 34 3.5 ENQUETES OU ETUDES PONCTUELLES CONCOURANT A LA SURVEILLANCE ................................................. 35 Mise en place de technique de génotypage de Trichosporon asahii ...............................................................................35 4 ALERTE .................................................................................................................................... 37 Cas groupés d’infections à Magnusiomyces capitatus ....................................................................................................37 5 ACTIVITES DE RETRO-INFORMATION, DE FORMATION ET DE CONSEIL....................................... 38 5.1 CONSEIL ET EXPERTISE AUX PROFESSIONNELS DE SANTE ........................................................................ 38 5.2 CONSEIL ET EXPERTISE AUX AUTORITES SANITAIRES .............................................................................. 39 6 TRAVAUX DE RECHERCHE ET PUBLICATIONS EN LIEN DIRECT AVEC L’ACTIVITE DU CNR ............. 40 6.1 ACTIVITES DE RECHERCHE EN 2017 EN LIEN DIRECT AVEC LES MISSIONS ET ACTIVITES DU CNR .................... 40 6.1.1 Études concernant la cryptococcose ..................................................................................... 40 Etude PreCASA.................................................................................................................................................................40 Etude ACTA ......................................................................................................................................................................40 6.1.2 Études concernant la pneumocystose .................................................................................. 41 6.1.3 Études concernant les candidoses et autres fongémies à levures ........................................ 42 Fongémies dues aux espèces fréquentes de Candida spp. ..............................................................................................42 Fongémies dues aux levures rares ...................................................................................................................................42 Endocardites à Candida spp. ...........................................................................................................................................43 Traitement des candidémies sévères. .............................................................................................................................43 Traitement des péritonites à Candida spp. .....................................................................................................................43 6.1.4 Aspergilloses et autres infections à champignons filamenteux ............................................ 43 6.1.5 Études concernant les techniques d’identification et la phylogénie ..................................... 44 Rapport d’activité – CNRMA2017 avril 2018 Page 2 sur 64 Amélioration des techniques d’identification des champignons « barcoding » ..............................................................44 Analyse des souches du genre Fusarium .........................................................................................................................44 Étude Européen sur la diversité de coelomycètes responsables des infections chez l’homme ......................................44 6.2 LISTE DES PUBLICATIONS ET COMMUNICATIONS EN LIEN DIRECT AVEC LES MISSIONS ET ACTIVITES DU CNR ... 45 6.2.1 Publications nationales ......................................................................................................... 45 6.2.2 Publications internationales ................................................................................................. 45 6.2.3 Communications nationales ................................................................................................. 47 6.2.4 Communications internationales .......................................................................................... 47 6.2.5 Conférences sur invitations ................................................................................................... 48 7 COOPERATION AVEC LES LABORATOIRES DE SANTE ANIMALE, D’HYGIENE ALIMENTAIRE, ENVIRONNEMENTAUX..................................................................................................................... 48 8 PROGRAMME D’ACTIVITE POUR LES ANNEES SUIVANTES ......................................................... 48 9 LISTE DES COLLABORATEURS DU CNRMA ................................................................................. 49 9.1 CC-CNRMA FORMANT RESSIF....................................................................................................... 49 9.2 CENTRES PARTICIPANTS A L’ODL ...................................................................................................... 49 9.3 PARTICIPANTS A LA SURVEILLANCE DES MYCOSES INVASIVES EN 2017 ..................................................... 49 ANNEXE
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