The Virus Diagnostic Laboratory: Functions and Problems Based on Three Years’ Experience 339
Total Page:16
File Type:pdf, Size:1020Kb
Load more
Recommended publications
-
Nasoswab ® Brochure
® NasoSwab One Vial... Multiple Pathogens Simple & Convenient Nasal Specimen Collection Medical Diagnostic Laboratories, L.L.C. 2439 Kuser Road • Hamilton, NJ 08690-3303 www.mdlab.com • Toll Free 877 269 0090 ® NasoSwab MULTIPLE PATHOGENS The introduction of molecular techniques, such as the Polymerase Chain Reaction (PCR) method, in combination with flocked swab technology, offers a superior route of pathogen detection with a high diagnostic specificity and sensitivity. MDL offers a number of assays for the detection of multiple pathogens associated with respiratory tract infections. The unrivaled sensitivity and specificity of the Real-Time PCR method in detecting infectious agents provides the clinician with an accurate and rapid means of diagnosis. This valuable diagnostic tool will assist the clinician with diagnosis, early detection, patient stratification, drug prescription, and prognosis. Tests currently available utilizing ® the NasoSwab specimen collection platform are listed below. • One vial, multiple pathogens Acinetobacter baumanii • DNA amplification via PCR technology Adenovirus • Microbial drug resistance profiling Bordetella parapertussis • High precision robotic accuracy • High diagnostic sensitivity & specificity Bordetella pertussis (Reflex to Bordetella • Specimen viability up to 5 days after holmesii by Real-Time PCR) collection Chlamydophila pneumoniae • Test additions available up to 30 days Coxsackie virus A & B after collection • No refrigeration or freezing required Enterovirus D68 before or after collection -
Follow-Up of COVID-19 Recovered Patients with Mild Disease
www.nature.com/scientificreports OPEN Follow‑up of COVID‑19 recovered patients with mild disease Alina Kashif1, Manahil Chaudhry2, Tehreem Fayyaz1, Mohammad Abdullah1*, Ayesha Malik2, Javairia Manal Akmal Anwer1, Syed Hashim Ali Inam1, Tehreem Fatima2, Noreena Iqbal3 & Khadija Shoaib1 COVID‑19 may manifest as mild, moderate or severe disease with each grade of severity having its own features and post‑viral implications. With the rising burden of the pandemic, it is vital to identify not only active disease but any post‑recovery complications as well. This study was conducted with the aim of identifying the presence of post‑viral symptomatology in patients recovered from mild COVID‑19 disease. Presence or absence of 11 post‑viral symptoms was recorded and we found that 8 of the 11 studied symptoms were notably more prevalent amongst the female sample population. Our results validate the presence of prolonged symptoms months after recovery from mild COVID‑19 disease, particularly in association with the female gender. Hence, proving the post‑COVID syndrome is a recognizable diagnosis in the bigger context of the post‑viral fatigue syndrome. Te SARS-CoV-2 virus has led to a global health crisis ever since the frst case of COVID-19 was reported in November 2012 in Wuhan, China1. COVID-19 primarily targets the respiratory system with variable initial symptoms including fever, sore throat, fu-like illness, and diarrhea 2. Tere is a chance that some symptoms may linger even afer the convalescence phase has subsided. Te presence of symptoms afer recovery from a viral disease is broadly recognized as a post-viral syndrome3. -
Coxsackie B Virus Infection As a Rare Cause of Acute Renal Failure and Hepatitis Thapa J, Koirala P, Gupta TN
Case Note VOL. 16|NO. 1|ISSUE 61|JAN.-MARCH. 2018 Coxsackie B Virus Infection as a Rare Cause of Acute Renal Failure and Hepatitis Thapa J, Koirala P, Gupta TN Department of Nephrology National Academy of Medical Sciences, Bir Hospital, Kathmandu, Nepal. ABSTRACT We report a 37 year female patient, admitted with complains of fever, jaundice and myalgia of seven days. There was no history of trauma, drug abuse, seizure or Corresponding Author vigorous exercise nor history of renal and musculoskeletal disease. Here we have Jiwan Thapa discussed the clinical features, biochemical derangements, diagnosis of coxsackie B virus, multi organ involvement and need of urgent hemodialysis for appropriate Department of Nephrology management of the case. National Academy of Medical Sciences, Bir Hospital, Kathmandu, Nepal. KEY WORDS E-mail: [email protected] Acute renal failure, Coxsackie B, Hemodialysis, Hepatitis Citation Thapa T, Koirala P, Gupta TN. Coxsackie B Virus Infection as a rare cause of Acute Renal Failure and Hepatitis. Kathmandu Univ Med J. 2018;61(1):95-7. INTRODUCTION CASE REPORT The Coxsackie viruses are Ribonucleic acid viruses of A previously healthy 37-year-old female was admitted to the Picornaviridae family, Enterovirus genus which also our hospital with complains of fever of 7 days, jaundice includes echoviruses and polioviruses. Infections are of 4 days, severe muscle pain, especially the lower limbs, mostly asymptomatic. They are divided into groups A and discoloured urine. She also had history of malaise, and B. Coxsackie virus A virus usually affects skin and headache, sore throat and fever recorded up to 103.2°F. -
International Respiratory Infections Society COVID Research Conversations: Podcast 3 with Dr
University of Louisville Journal of Respiratory Infections MULTIMEDIA International Respiratory Infections Society COVID Research Conversations: Podcast 3 with Dr. Antoni Torres Julio A. Ramirez1*, MD, FACP; Antoni Torres, MD, PhD, FERS 1Center of Excellence for Research in Infectious Diseases, Division of Infectious Diseases, University of Louisville, Louisville, KY; 2Department of Pulmonology and Critical Care, University of Barcelona *[email protected] Recommended Citation: Ramirez JA, Torres A. International Respiratory Infections Society COVID Research Conversations: Podcast 3 with Dr. Antoni Torres. Univ Louisville J Respir Infect 2021; 5(1): Article 11. Outline of Discussion Topics Section(s) Topics 1–4 Introductions 5 “Spanish” influenza 6–9 Dr. Torres’ personal thoughts and experiences 10 COVID-19 hospitalizations in Barcelona 11 A threatening phone call 12–13 Origin of the CIBERESUCICOVID project 14 Baseline characteristics 15 Bloodwork at hospital admission; ICU admission vs. day 3 16 Treatments 17 Complications 18 Outcomes related to interventions 19 Viral RNA load in plasma associated with critical illness and dysregulated response 20 Follow-up with health care workers 21 Medical education 22 Conclusions 23–26 Interleukin 6 27–29 Ventilatory approach 30–33 Post-COVID syndrome 34–38 Impact on health care workers 39–41 Holidays and COVID-19 infection 42–43 New paradigm for medical education 44–45 Reducing travel for medical conferences 46–47 Improving treatment for COVID-19 48–53 Vaccination in Spain 54–57 Prioritizing clinical trials 58–65 COVID-19 as viral pneumonia 64–69 Thanks and sign-off All figures kindly provided by Dr. Torres. ULJRI j https://ir.library.louisville.edu/jri/vol5/iss1/11 1 ULJRI International Respiratory Infections Society COVID Research Conversations: Podcast 3 with Dr. -
Coxsackie B Infection and Arthritis Arsenical Treatment for Multiple
Br Med J (Clin Res Ed): first published as 10.1136/bmj.286.6365.605 on 19 February 1983. Downloaded from BRITISH MEDICAL JOURNAL VOLUME 286 19 FEBRUARY 1983 605 patients with febrile arthritis developing in association with Coxsackie Coxsackie B infection and arthritis infection has been reported previously4; two of those patients were clinically similar to two of ours (cases 2 and 3). The patient in case 1, The clinical manifestations of acute infection with Coxsackie B virus however, who was positive for HLA-B27 and had a history suggestive are varied and include epidemic pleurodynia, myopericarditis, of pre-existing mild sacroiliitis, did not resemble the previously meningoencephalitis, and pancreatitis. In most cases the infection is reported cases. Although he may represent an example of reactive self limiting and does not result in chronic tissue damage, but it has arthritis in response to Coxsackie infection in a patient with HLA-B27, also been associated with the development of polymyositis,l cardio- we cannot exclude the possibility that direct infection of joints by myopathy,' and diabetes mellitus.3 Arthritis is not widely recognised virus occurred. as either an acute or a chronic manifestation of infection with Coxsackie infection should be considered in the differential diagnosis Coxsackie virus, and only one series, of six patients, has been reported in patients presenting with febrile systemic illness in association with previously.4 We report on three further patients, who developed seronegative arthritis of either symmetrical or asymmetrical patterns, febrile seronegative arthritis in association with clinical and serological with or without spondarthritis. evidence of Coxsackie infection; one subsequently developed a We thank the Edinburgh and South-east Scotland Blood Transfusion progressive erosive polyarthritis. -
Required Chlorination Doses to Fulfill the Credit Value for Disinfection of Enteric Viruses in Water: a Critical Review
Required chlorination doses to fulfill the credit value for disinfection of enteric viruses in water: A critical review Item Type Article Authors Rachmadi, Andri Taruna; Kitajima, Masaaki; Kato, Tsuyoshi; Kato, Hiroyuki; Okabe, Satoshi; Sano, Daisuke Citation Rachmadi, A. T., Kitajima, M., Kato, T., Kato, H., Okabe, S., & Sano, D. (2020). Required chlorination doses to fulfill the credit value for disinfection of enteric viruses in water: A critical review. Environmental Science & Technology. doi:10.1021/ acs.est.9b01685 Eprint version Publisher's Version/PDF DOI 10.1021/acs.est.9b01685 Publisher American Chemical Society (ACS) Journal Environmental Science & Technology Rights This is an open access article published under an ACS AuthorChoice License, which permits copying and redistribution of the article or any adaptations for non-commercial purposes. Download date 02/10/2021 21:39:20 Item License http://pubs.acs.org/page/policy/authorchoice_termsofuse.html Link to Item http://hdl.handle.net/10754/661375 This is an open access article published under an ACS AuthorChoice License, which permits copying and redistribution of the article or any adaptations for non-commercial purposes. pubs.acs.org/est Critical Review Required Chlorination Doses to Fulfill the Credit Value for Disinfection of Enteric Viruses in Water: A Critical Review Andri Taruna Rachmadi, Masaaki Kitajima, Tsuyoshi Kato, Hiroyuki Kato, Satoshi Okabe, and Daisuke Sano* Cite This: https://dx.doi.org/10.1021/acs.est.9b01685 Read Online ACCESS Metrics & More Article Recommendations *sı Supporting Information ABSTRACT: A credit value of virus inactivation has been assigned to the disinfection step in international and domestic guidelines for wastewater reclamation and reuse. -
The Medical and Public Health Importance of the Coxsackie Viruses
806 S.A. MEDICAL JOURNAL 25 August 1956 it seems that this mother's claim cannot be upheld, Dit lyk dus of hierdie moeder se eis nie gestaaf kan word and we must still look for further evidence of human nie en ons moet nog steeds soek na verdere bewyse van parthenogenesis. menslike partenogenese. 1. Editorial (1955): Lancet, 2, 967. 1. Van die Redaksie (1955): Lancet, 2, 967. 2. Pincus, G. and Shapiro, H. (1939): Proc. Nat. Acad. Sci., 2. Pincus, G. en Shapiro, H. (1939): Proc. Nat. Acad. Sci., 26,163. 26, 163. 3. Balfour-Lynn, S. (1956): Lancet, 1, 1072. 3. Balfour-Lynn, S. (1956): Lancet, 1, 1072. THE MEDICAL AND PUBLIC HEALTH IMPORTANCE OF THE COXSACKIE VIRUSES JA.\1ES GEAR, V.MasRoCH M'D F. R. PRINSLOO Poliomyelitis Research Foundation, South African Institute for Medical Research The Coxsackie group of viruses derived their name paralytic poliomyelitis infected also with poliovirus. from the Hudson River Town, Coxsackie, in New York As a result of more recent studies their pathogenicity State, where the first two members of this group were has now been more clearly defined. 2 The group-A identified by Dalldorf and Sickies in 1947.1 Both these viruses have been incriminated as the cause of herp viruses were isolated in suckling mice from the faeces angina and, as the present studies show, are possibly of children acutely ill with paralytic poliomyelitis. The related to a number of other illnesses. Coxsackie-B pathogenicity for suckling mice is one of the distinguish viruses have been incriminated as the cause of Bornholm ing features of this group of viruses, and their relative disease and, as the present studies show, in parts of lack of pathogenicity for adult mice and other experi Southern Africa are important causes ofaseptic meningo mental animals accounts for their escape from recogni encephalitis and of myocarditis neonatorum. -
Assessment of Enteroviruses from Sewage Water and Clinical Samples During Eradication Phase of Polio in North India Sarika Tiwari1,2* and Tapan N
Tiwari and Dhole Virology Journal (2018) 15:157 https://doi.org/10.1186/s12985-018-1075-7 RESEARCH Open Access Assessment of enteroviruses from sewage water and clinical samples during eradication phase of polio in North India Sarika Tiwari1,2* and Tapan N. Dhole1 Abstract Background: The Enterovirus (EV) surveillance system is inadequate in densely populated cities in India. EV can be shed in feces for several weeks; these viruses are not easily inactivated and may persist in sewage for long periods. Surveillance and epidemiological study of EV-related disease is necessary. Methods: In this study, we compare the EV found in sewage with clinically isolated samples. Tissue culture was used for isolation of the virus and serotype confirmed by enterovirus neutralization tests. Results: We found positive cases for enterovirus from clinical and sewage samples and identified additional isolates as echovirus 9, 11, 25 & 30 by sequencing. Conclusion: There is a close relation among the serotypes of enterovirus shed in stools and isolated from the environment but few serotypes which were detected in sewage samples were not found clinically and the few which were detected clinically not found in sewage because some viruses are difficult to detect by the cell culture method.This study will be helpful for the researchers who are working on polio and nonpolio enterovirus especially in the countries which are struggling for polio eradication. Keywords: Acute flaccid paralysis, Environmental surveillance, Phylogenetic analysis, Sewage water Background than 100 serotypes have been identified [3] including more Enterovirus can be transported in the environment than 70 enterovirus serotypes have been identified in through groundwater, estuarine water, seawater, rivers, humans [4]. -
Trajectory of the COVID-19 Pandemic: Chasing a Moving Target
1 Review Article Page 1 of 13 Trajectory of the COVID-19 pandemic: chasing a moving target Kamal Kant Sahu1, Ajay Kumar Mishra1, Amos Lal2^ 1Department of Internal Medicine, Saint Vincent Hospital, Worcester, Massachusetts, 01608, USA; 2Division of Pulmonary and Critical Care Medicine, Mayo Clinic, Rochester, Minnesota 55902, USA Contributions: (I) Conception and design: All authors; (II) Administrative support: None; (III) Provision of study materials or patients: All authors; (IV) Collection and assembly of data: All authors; (V) Data analysis and interpretation: All authors; (VI) Manuscript writing: All authors; (VII) Final approval of manuscript: All authors. Correspondence to: Amos Lal, MBBS, MD. Division of Pulmonary and Critical Care Medicine, Mayo Clinic, Rochester, Minnesota 55902, USA. Email: [email protected]; [email protected]. Abstract: The spread of COVID-19 has already taken a pandemic form, affecting over 180 countries in a matter of three months. The full continuum of disease ranges from mild, self-limiting illness to severe progressive COVID-19 pneumonia, multiorgan failure, cytokine storm and death. Younger and healthy population is now getting affected than before. Possibilities of airborne and fecal oral routes of transmission has increased the concern. In the absence of any specific therapeutic agent for coronavirus infections, the most effective manner to contain this pandemic is probably the non-pharmacological interventions (NPIs). The damage due to the pandemic disease is multifaceted and crippling to economy, trade, and health of the citizens of the countries. The extent of damage in such scenarios is something that is beyond calculation by Gross Domestic Product rate or currency value of the country. -
Epidemic Coxsackie B Virus Infection in Johannesburg, South Africa
J. Ilyg., Camb. (1985), 95, 447-455 447 Printed in Great Britain Epidemic Coxsackie B virus infection in Johannesburg, South Africa BY BARRY D. SCHOUB, SYLVIA JOHNSON, JO M. McANERNEY, ISABEL L. DOS SANTOS AND KATALIN I. M. KLAASSEN National Institute for Virology and Department of Virology, University of the Witivalersrand, South Africa (Received 4 April 1985; accepted 31 May 1985) SUMMARY A particularly extensive epidemic of Coxsackie B3 virus infection occurred in Johannesburg in the spring and summer of 1984. A total of 142 positive cases were diagnosed by isolation of the virus from stools and other specimens (60) or by serology (82). Coxsackie B3 accounted for 87 % of the isolations and was also the dominant serotypc on serology. The outbreak involved predominantly children and young adults, with no apparent sex differences being noted. The majority of specimens came from the white population and no significant difference in age or sex distribution could be observed between the two race groups. The major clinical presentation in the white group was Bornholm disease followed by cardiac involvement and then meningo- encephalitis. In the black group, however, myocarditis was the major clinical presentation, which is of particular interest taking into account the extremely high incidence of acute rheumatic carditis in this population and the prevalence of chronic cardiomyopathy. INTRODUCTION Epidemics of Coxsackie B virus infection have occurred at regular intervals in Johannesburg since the first documented outbreak of neonatal disease in a niaternity homo in October and November 1952 (Javctt el al. 195G; Gear, 19G7). In temperate countries, as well as in South Africa, these outbreaks occur characteristically in the warmer spring to autumn months in cycles of some 3- to 0-year intervals (Gear, 1967; Melnick, 1982; Banatvala, 1983). -
H1N1 Influenza Causing Fulminant Myocarditis Requiring Extracorporeal Membrane Oxygenation
Open Access Case Report DOI: 10.7759/cureus.4665 H1N1 Influenza Causing Fulminant Myocarditis Requiring Extracorporeal Membrane Oxygenation Ali Hamoudi 1 , Dana Vais 2 , Vian Taqi 3 1. Internal Medicine, Chicago Medical School / Rosalind Franklin University of Medicine and Science, Chicago, USA 2. Infectious Disease, AMITA Saints Mary and Elizabeth Medical Center / Rosalind Franklin University of Medicine and Science, Chicago, USA 3. Internal Medicine, University of Baghdad, Baghdad, IRQ Corresponding author: Ali Hamoudi, [email protected] Abstract Influenza infection is a known cause of global morbidity and mortality. Most cases of influenza A (H1N1) influenza infection are mild and do not require hospitalization. Although the most common presentation is with upper respiratory tract symptoms, hemodynamic instability requiring vasoactive drugs and ventilatory support use is unusual. We present a case of acute fulminant myocarditis that presented with dyspnea, which was confirmed with laboratory tests, chest X-ray, and echocardiogram. The test for H1N1 in nasopharyngeal secretions was positive. The patient evolved to refractory cardiogenic shock despite the clinical measures applied. Categories: Cardiology, Internal Medicine, Infectious Disease Keywords: h1n1, myocarditis, ecmo, vasopressors Introduction Acute myocarditis is defined as an inflammation of the myocardial muscle, causing myocytes necrosis and inflammatory infiltrate of the heart muscle. The most common etiology is attributed to viral infections like Coxsackie B virus and adenovirus. Many people are affected by seasonal influenza every year, causing symptoms that are limited to the respiratory system. Myocardial involvement by seasonal influenza virus seems to be low [1-2]. The frequency of myocarditis secondary to seasonal influenza virus infection ranges from 0%-11%, depending on the criteria used to diagnose myocarditis, however, the prevalence of influenza A (H1N1) myocarditis remains unclear [3]. -
HUMAN ADENOVIRUS Credibility of Association with Recreational Water: Strongly Associated
6 Viruses This chapter summarises the evidence for viral illnesses acquired through ingestion or inhalation of water or contact with water during water-based recreation. The organisms that will be described are: adenovirus; coxsackievirus; echovirus; hepatitis A virus; and hepatitis E virus. The following information for each organism is presented: general description, health aspects, evidence for association with recreational waters and a conclusion summarising the weight of evidence. © World Health Organization (WHO). Water Recreation and Disease. Plausibility of Associated Infections: Acute Effects, Sequelae and Mortality by Kathy Pond. Published by IWA Publishing, London, UK. ISBN: 1843390663 192 Water Recreation and Disease HUMAN ADENOVIRUS Credibility of association with recreational water: Strongly associated I Organism Pathogen Human adenovirus Taxonomy Adenoviruses belong to the family Adenoviridae. There are four genera: Mastadenovirus, Aviadenovirus, Atadenovirus and Siadenovirus. At present 51 antigenic types of human adenoviruses have been described. Human adenoviruses have been classified into six groups (A–F) on the basis of their physical, chemical and biological properties (WHO 2004). Reservoir Humans. Adenoviruses are ubiquitous in the environment where contamination by human faeces or sewage has occurred. Distribution Adenoviruses have worldwide distribution. Characteristics An important feature of the adenovirus is that it has a DNA rather than an RNA genome. Portions of this viral DNA persist in host cells after viral replication has stopped as either a circular extra chromosome or by integration into the host DNA (Hogg 2000). This persistence may be important in the pathogenesis of the known sequelae of adenoviral infection that include Swyer-James syndrome, permanent airways obstruction, bronchiectasis, bronchiolitis obliterans, and steroid-resistant asthma (Becroft 1971; Tan et al.