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Redalyc.ETHANOL PRODUCTION by Zymomonas Mobilis NRRL B-806 from ENZYMATIC HYDROLYSATES of Eucalyptus Globulus

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Redalyc.ETHANOL PRODUCTION by Zymomonas Mobilis NRRL B-806 from ENZYMATIC HYDROLYSATES of Eucalyptus Globulus Revista Mexicana de Ingeniería Química ISSN: 1665-2738 [email protected] Universidad Autónoma Metropolitana Unidad Iztapalapa México Morales-Martínez, T.K.; Rios-Gonzalez, L.J.; Aroca-Arcaya, G.; Rodríguez-de la Garza, J.A. ETHANOL PRODUCTION BY Zymomonas mobilis NRRL B-806 FROM ENZYMATIC HYDROLYSATES OF Eucalyptus globulus Revista Mexicana de Ingeniería Química, vol. 13, núm. 3, 2014, pp. 779-785 Universidad Autónoma Metropolitana Unidad Iztapalapa Distrito Federal, México Available in: http://www.redalyc.org/articulo.oa?id=62035738011 How to cite Complete issue Scientific Information System More information about this article Network of Scientific Journals from Latin America, the Caribbean, Spain and Portugal Journal's homepage in redalyc.org Non-profit academic project, developed under the open access initiative Revista Mexicana de Ingeniería Química Vol. 13, No.CONTENIDO 3 (2014) 779-785 Volumen 8, número 3, 2009 / Volume 8, number 3, 2009 ETHANOL PRODUCTION BY Zymomonas mobilis NRRL B-806 FROM ENZYMATIC HYDROLYSATES OF Eucalyptus globulus 213 Derivation and application of the Stefan-Maxwell equations PRODUCCION´ DE ETANOL A PARTIR DE HIDROLIZADOS ENZIMATICOS´ DE Eucalyptus (Desarrollo globulus y aplicaciónUSANDO de las ecuacionesZymomonas de Stefan-Maxwell) mobilis NRRL B-806 Stephen Whitaker 1 1∗ 2 1 T.K. Morales-Mart ´ınez , L.J. Rios-Gonzalez´ , G. Aroca-Arcaya and J.A. Rodr´ıguez-de la Garza 1Departamento de Biotecnolog´ıa,Facultad de Ciencias Qu´ımicas,Universidad Aut´onomade Coahuila, Blvd. V. Biotecnología / Biotechnology Carranza, Z.C. 25280, Saltillo, Coahuila, M´exico 2Escuela de Ingenier´ıaBioqu´ımica,Pontificia245 Modelado de la biodegradación Universidad en biorreactores Cat´olicade de lodos de hidrocarburos Valpara´ıso,Gral. totales del Cruzpetróleo 34, Valpara´ıso, intemperizados en suelos y sedimentosChile. (BiodegradationReceived Marchmodeling 3,of sludge 2014; bioreactors Accepted of total September petroleum hydrocarbons 26, 2014 weathering in soil and sediments) Abstract S.A. Medina-Moreno, S. Huerta-Ochoa, C.A. Lucho-Constantino, L. Aguilera-Vázquez, A. Jiménez- González y M. Gutiérrez-Rojas Ethanol production by Zymomonas mobilis NRRL-806 was assessed using different enzymatic hydrolysates from pretreated 259 Crecimiento, sobrevivencia y adaptación de Bifidobacterium infantis a condiciones ácidas Eucalyptus globulus saw dust pulp. Results showed that when enzymatic hydrolysate containing 79.5 g L−1 of glucose and 8.81 g L−1 of acetic acid, a(Growth, maximum survival ethanol and adaptation yield of Bifidobacterium 92% and a productivity infantis to acidic of conditions) 1.16 g L−1 h−1 were obtained, with a total of 37 g L−1 of ethanolL. after Mayorga-Reyes, 27 h. Acetic P. Bustamante-Camilo, acid concentration A. Gutiérrez-Nava, present in enzymaticE. Barranco-Florido hydrolysates y A. Azaola- was the main factor that contributed to decreaseEspinosa ethanol yield and productivity, in the case of hydrolysate E, where acetic acid concentration was higher (12.8 g L−1),265 ethanol Statistical yield approach and productivityto optimization were of ethanol 80% fermentation and 0.99 g by L −Saccharomyces1 h−1 respectively. cerevisiae in the Keywords: Eucalyptus globuluspresence, enzymatic of Valfor® hydrolysates,zeolite NaA Zymomonas mobilis NRRL-806, ethanol yield, acetic acid. Resumen (Optimización estadística de la fermentación etanólica de Saccharomyces cerevisiae en presencia de Se evaluo´ la produccion´ dezeolita etanol Valfor® por Zymomonas zeolite NaA) mobilis NRRL-806 a partir de diferentes hidrolizados enzimaticos´ de pulpa de aserr´ın de EucalyptusG. Inei-Shizukawa, globulus pretratada. H. A. Velasco-Bedrán, Los resultados G. F. Gutiérrez-López mostraron and un H. m Hernández-Sánchezaximo´ rendimiento de etanol de 92% y una productividad de 1.16 g L−1 h−1, con una concentracion´ total de etanol de 37 g L−1 despues´ de 27 h, cuando el hidrolizado enzimatico´ Ingeniería conten de´ıa procesos 79.5 g / Process L−1 de engineering glucosa y 8.81 g L−1 de acido´ acetico.´ La concentracion´ de acido´ acetico´ presente en los hidrolizados271 Localización enzim de unaaticos´ plantafue industrial: el principal Revisión factor crítica quey adecuación contribuy de olos´ a criterios la disminuci empleadoson´ en del rendimiento y productividad de etanol, enesta el caso decisión del hidrolizado E, en donde la concentracion´ de acido´ acetico´ fue mayor (12.8 g L−1), el rendimiento y la productividad (Plant site de selection: etanol fueronCritical review de 80% and yadequation 0.99 g Lcriteria−1 h −used1 respectivamente. in this decision) J.R. Medina, R.L. Romero y G.A. Pérez Palabras clave: Eucalyptus globulus, hidrolizados enzimaticos,´ Zymomonas mobilis NRRL-806, rendimiento de etanol, acido´ acetico.´ 1 Introduction The imminent ending of the non-renewable natural biomass is the most abundant renewable biological energetic resources brings the necessity to research resource and, since is outside the human food chain for new renewable natural energetic resources, as well is also an attractive and relatively inexpensive raw as the maximization of productivity and lowering the material (Pereira et al., 2012). Lignocellulose costs of already known processes. Biomass-based is an abundant natural carbohydrate formed by energy sources constitute an important alternative to biopolymers such as cellulose, hemicellulose and help solving this problem (Letti and Karp, 2012; lignin, which can be transformed into substitute Dom´ınguez-Maldonado et al., 2014). Lignocellulosic renewable energy resource by microbial conversion ∗Corresponding author. E-mail: [email protected] Tel.: +52 (844) 4155752, Fax: +52 (844) 4159534 Publicado por la Academia Mexicana de Investigacion´ y Docencia en Ingenier´ıa Qu´ımica A.C. 779 Morales-Mart´ınezet al./ Revista Mexicana deIngenier ´ıaQu ´ımica Vol. 13, No. 3 (2014) 779-785 (Roberto et al., 2003). The advantages of using 2 Materials and methods them as raw materials for ethanol production are their low cost, easy availability, avoidance of 2.1 Microorganism and media conflict with their use for food, and the potential production of fuel from lignin (Liu et al., 2012). Zymomonas mobilis NRRL B-806 strain was However, the heterogeneous and complex structure of provided by the Environmental Biotechnology Lab. lignocellulosic makes their fractionation and further of Biochemistry Engineering Faculty, Pontificia benefit difficult (Roman´ı et al., 2012). The main Universidad Catolica de Valparaiso, Chile (PUCV). stages of the cellulosic ethanol production process are Zymomonas mobilis NRRL B-806 was cultivated on pre-treatment, hydrolysis, fermentation, distillation, M2 solid medium containing per liter: 100 g glucose, and further fuel upgrading (Starfelt et al., 2012). 5 g yeast extract, 2 g KH2PO4, 1 g MgSO4 and 1.5 There are at least three methods of hydrolysis, g (NH4)2SO4 at 30 ºC. Inoculum was prepared from including dilute acid hydrolysis, concentrated acid the stock culture, using the same culture medium. The hydrolysis, and enzymatic hydrolysis. The enzyme- growth was carried out at 30 ºC for 48 hours. based process is more environmental-friendly and gives higher hydrolysis yield than acid hydrolysis 2.2 Eucalyptus globulus pretreatment (Zheng et al., 2009). Therefore enzymatic hydrolysis Eucalyptus globulus saw dust pulp pretreatment was is advantageous because of its low toxicity, low utility carried out in a stainless steel reactor under the cost and low corrosion compared to acid or alkaline following conditions: H SO 1% at 176 °C for hydrolysis (Sun and Cheng, 2002; Taherzadeh and 2 4 10 minutes followed by a quick decompression to Karimi, 2007). In enzymatic hydrolysis the enzymes atmospheric pressure. The solids were separated by catalyze only specific reactions, and as result no other filtering and washed with tap water to neutrality. The side reactions occur or byproducts are formed and solid residue was dried in a forced-air oven at 105 °C. hydrolysis has the potential to achieve higher yield of reducing sugars (Mukhopadhyay et al., 2011). Here cellulase and hemicellulase enzymes cleave the 2.3 Enzymatic hydrolysates bonds of cellulose and hemicellulose respectively. The study was carried out using three different Cellulase enzymes involve endo and exoglucanase enzymatic hydrolysates from pretreated Eucalyptus and β-glucosidases. Hemicellulolytic enzymes are globulus saw dust pulp under the following conditions: more complex and are a mixture of at least eight (FPU:UI pNPG ratio and enzyme/sustrate relation). enzymes. Cellulose is hydrolysed to glucose whereas Enzymes used were of the series NS500 Novozymes hemicellulose gives rise to several pentoses and (Bagsvaerd Denmark), cellulase (NS50013) and β- hexoses (Sarkar et al., 2012). glucosidase (NS50010). Enzymatic hydrolysates compositions are shown in Table 1 obtained under the following conditions; 50 °C, with a stirring rate of 200 Zymomonas mobilis has been intensively studied rpm during 72 h. in ethanol fermentation. Zymomonas mobilis metabolizes sugar via the Entner-Doudoroff (ED) 2.4 Hydrolysates fermentation process pathway, which produces less ATP and less biomass. More carbon sources are thus channeled to ethanol, Enzymatic hydrolysates were supplemented with resulting in an even higher ethanol yield than that nutrients of M2 medium at a pH 5.5 (without glucose). found with the native ethanol fermenting yeast Experiments were carried out in 250 mL Erlenmeyer Saccharomyces cerevisiae. In addition, because of
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